PRIMER_SEQUENCE_ID=NM_000061
SEQUENCE=CTCAGACTGTCCTTCCTCTCTGGACTGTAAGAATATGTCTCCAGGGCCAGTGTCTGCTGCGATCGAGTCCCACCTTCCAAGTCCTGGCATCTCAATGCATCTGGGAAGCTACCTGCATTAAGTCAGGACTGAGCACACAGGTGAACTCCAGAAAGAAGAAGCTATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGAGAAGAGGCAGTAAGAAGGGTTCAATAGATGTTGAGAAGATCACTTGTGTTGAAACAGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCGAGAAGAGGTGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCTTATCCCTTCCAGGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCCAACTGAAGAACTAAGGAAGCGGTGGATTCACCAGCTCAAAAACGTAATCCGGTACAACAGTGATCTGGTTCAGAAATATCACCCTTGCTTCTGGATCGATGGGCAGTATCTCTGCTGCTCTCAGACAGCCAAAAATGCTATGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCTCACCGGAAGACAAAAAAGCCTCTTCCCCCAACGCCTGAGGAGGACCAGATCTTGAAAAAGCCACTACCGCCTGAGCCAGCAGCAGCACCAGTCTCCACAAGTGAGCTGAAAAAGGTTGTGGCCCTTTATGATTACATGCCAATGAATGCAAATGATCTACAGCTGCGGAAGGGTGATGAATATTTTATCTTGGAGGAAAGCAACTTACCATGGTGGAGAGCACGAGATAAAAATGGGCAGGAAGGCTACATTCCTAGTAACTATGTCACTGAAGCAGAAGACTCCATAGAAATGTATGAGTGGTATTCCAAACACATGACTCGGAGTCAGGCTGAGCAACTGCTAAAGCAAGAGGGGAAAGAAGGAGGTTTCATTGTCAGAGACTCCAGCAAAGCTGGCAAATATACAGTGTCTGTGTTTGCTAAATCCACAGGGGACCCTCAAGGGGTGATACGTCATTATGTTGTGTGTTCCACACCTCAGAGCCAGTATTACCTGGCTGAGAAGCACCTTTTCAGCACCATCCCTGAGCTCATTAACTACCATCAGCACAACTCTGCAGGACTCATATCCAGGCTCAAATATCCAGTGTCTCAACAAAACAAGAATGCACCTTCCACTGCAGGCCTGGGATACGGATCATGGGAAATTGATCCAAAGGACCTGACCTTCTTGAAGGAGCTGGGGACTGGACAATTTGGGGTAGTGAAGTATGGGAAATGGAGAGGCCAGTACGACGTGGCCATCAAGATGATCAAAGAAGGCTCCATGTCTGAAGATGAATTCATTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTGCAGTTGTATGGCGTCTGCACCAAGCAGCGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGCTGCCTCCTGAACTACCTGAGGGAGATGCGCCACCGCTTCCAGACTCAGCAGCTGCTAGAGATGTGCAAGGATGTCTGTGAAGCCATGGAATACCTGGAGTCAAAGCAGTTCCTTCACCGAGACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAGGAGTTGTTAAAGTATCTGATTTCGGCCTGTCCAGGTATGTCCTGGATGATGAATACACAAGCTCAGTAGGCTCCAAATTTCCAGTCCGGTGGTCCCCACCGGAAGTCCTGATGTATAGCAAGTTCAGCAGCAAATCTGACATTTGGGCTTTTGGGGTTTTGATGTGGGAAATTTACTCCCTGGGGAAGATGCCATATGAGAGATTTACTAACAGTGAGACTGCTGAACACATTGCCCAAGGCCTACGTCTCTACAGGCCTCATCTGGCTTCAGAGAAGGTATATACCATCATGTACAGTTGTTGGCATGAGAAAGCAGATGAGCGTCCCACTTTCAAAATTCTTCTGAGCAATATTCTAGATGTCATGGATGAAGAATCCTGAGCTCGCCAATAAGCTTCTTGGTTCTACTTCTCTTCTCCACAAGCCCCAATTTCACTTTCTCAGAGGAAATCCCAAGCTTAGGAGCCCTGGAGCCTTTGTGCTCCCACTCAATACAAAAAGGCCCCTCTCTACATCTGGGGATGCACCTCTTCTTTGATTCCCTGGGATAGTGGCTTCTGAGCAAAGGCCAAAAAATTATTGTGCCTGAAATTTCCCGAGAGAATTAAGACAGACTGAATTTGCGATGAAAATATTTTTTAGGAGGGAGGATGTAAATAGCCGCACAAAGGGGTCCAACAGCTCTTTGAGTAGGCATTTGGTAGAGCTTGGGGGTGTGTGTGTGGGGGTGGACCGAATTTGGCAAGAATGAAATGGTGTCATAAAGATGGGAGGGGAGGGTGTTTTGATAAAATAAATTCTAGAAAGCTTAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 29253, GC content failed 48, low tm 13226, high tm 8698, high any compl 3, high end compl 14, high repeat similarity 29, long poly-x seq 85, ok 7150
PRIMER_RIGHT_EXPLAIN=considered 29209, GC content failed 303, low tm 13178, high tm 8512, high any compl 1, high end compl 7, high repeat similarity 7, long poly-x seq 91, ok 7110
PRIMER_PAIR_EXPLAIN=considered 294, unacceptable product size 249, high end compl 5, ok 40
PRIMER_PAIR_PENALTY=0.0612
PRIMER_LEFT_PENALTY=0.046971
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=GAGTATGACTTTGAACGTGGGAG
PRIMER_RIGHT_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT=283,23
PRIMER_RIGHT=1166,23
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=884
PRIMER_PAIR_PENALTY_1=0.0646
PRIMER_LEFT_1_PENALTY=0.046971
PRIMER_RIGHT_1_PENALTY=0.017590
PRIMER_LEFT_1_SEQUENCE=GAGTATGACTTTGAACGTGGGAG
PRIMER_RIGHT_1_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_1=283,23
PRIMER_RIGHT_1=911,23
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=629
PRIMER_PAIR_PENALTY_2=0.0762
PRIMER_LEFT_2_PENALTY=0.062009
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=TCACCTCTAAACTTCAAGAAGCG
PRIMER_RIGHT_2_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT_2=223,23
PRIMER_RIGHT_2=1166,23
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=944
PRIMER_PAIR_PENALTY_3=0.0772
PRIMER_LEFT_3_PENALTY=0.063026
PRIMER_RIGHT_3_PENALTY=0.014220
PRIMER_LEFT_3_SEQUENCE=GTCACTGAAGCAGAAGACTCCAT
PRIMER_RIGHT_3_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT_3=967,23
PRIMER_RIGHT_3=1166,23
PRIMER_LEFT_3_TM=59.937
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.0772
PRIMER_LEFT_4_PENALTY=0.063026
PRIMER_RIGHT_4_PENALTY=0.014220
PRIMER_LEFT_4_SEQUENCE=ATGTCACTGAAGCAGAAGACTCC
PRIMER_RIGHT_4_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT_4=965,23
PRIMER_RIGHT_4=1166,23
PRIMER_LEFT_4_TM=59.937
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=202
PRIMER_PAIR_PENALTY_5=0.0777
PRIMER_LEFT_5_PENALTY=0.063026
PRIMER_RIGHT_5_PENALTY=0.014712
PRIMER_LEFT_5_SEQUENCE=ATGTCACTGAAGCAGAAGACTCC
PRIMER_RIGHT_5_SEQUENCE=GCCATGTACTCAGTGATGATGAA
PRIMER_LEFT_5=965,23
PRIMER_RIGHT_5=1595,23
PRIMER_LEFT_5_TM=59.937
PRIMER_RIGHT_5_TM=60.015
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=631
PRIMER_PAIR_PENALTY_6=0.0777
PRIMER_LEFT_6_PENALTY=0.063026
PRIMER_RIGHT_6_PENALTY=0.014712
PRIMER_LEFT_6_SEQUENCE=GTCACTGAAGCAGAAGACTCCAT
PRIMER_RIGHT_6_SEQUENCE=GCCATGTACTCAGTGATGATGAA
PRIMER_LEFT_6=967,23
PRIMER_RIGHT_6=1595,23
PRIMER_LEFT_6_TM=59.937
PRIMER_RIGHT_6_TM=60.015
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=629
PRIMER_PAIR_PENALTY_7=0.0796
PRIMER_LEFT_7_PENALTY=0.062009
PRIMER_RIGHT_7_PENALTY=0.017590
PRIMER_LEFT_7_SEQUENCE=TCACCTCTAAACTTCAAGAAGCG
PRIMER_RIGHT_7_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_7=223,23
PRIMER_RIGHT_7=911,23
PRIMER_LEFT_7_TM=60.062
PRIMER_RIGHT_7_TM=60.018
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=10.2000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=689
PRIMER_PAIR_PENALTY_8=0.0829
PRIMER_LEFT_8_PENALTY=0.014712
PRIMER_RIGHT_8_PENALTY=0.068149
PRIMER_LEFT_8_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_8_SEQUENCE=TCCCATCTTTATGACACCATTTC
PRIMER_LEFT_8=1573,23
PRIMER_RIGHT_8=2533,23
PRIMER_LEFT_8_TM=60.015
PRIMER_RIGHT_8_TM=60.068
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=961
PRIMER_PAIR_PENALTY_9=0.0850
PRIMER_LEFT_9_PENALTY=0.067430
PRIMER_RIGHT_9_PENALTY=0.017590
PRIMER_LEFT_9_SEQUENCE=AGACTGTCCTTCCTCTCTGGACT
PRIMER_RIGHT_9_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_9=3,23
PRIMER_RIGHT_9=911,23
PRIMER_LEFT_9_TM=59.933
PRIMER_RIGHT_9_TM=60.018
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=909
PRIMER_PAIR_PENALTY_10=0.0875
PRIMER_LEFT_10_PENALTY=0.014712
PRIMER_RIGHT_10_PENALTY=0.072785
PRIMER_LEFT_10_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_10_SEQUENCE=AGACATCCTTGCACATCTCTAGC
PRIMER_LEFT_10=1573,23
PRIMER_RIGHT_10=1678,23
PRIMER_LEFT_10_TM=60.015
PRIMER_RIGHT_10_TM=59.927
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=106
PRIMER_PAIR_PENALTY_11=0.0938
PRIMER_LEFT_11_PENALTY=0.076178
PRIMER_RIGHT_11_PENALTY=0.017590
PRIMER_LEFT_11_SEQUENCE=CTGTCCTTCCTCTCTGGACTGTA
PRIMER_RIGHT_11_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_11=6,23
PRIMER_RIGHT_11=911,23
PRIMER_LEFT_11_TM=59.924
PRIMER_RIGHT_11_TM=60.018
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=906
PRIMER_PAIR_PENALTY_12=0.0945
PRIMER_LEFT_12_PENALTY=0.014712
PRIMER_RIGHT_12_PENALTY=0.079770
PRIMER_LEFT_12_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_12_SEQUENCE=ACAACTCCTTGATCGTTTACCAA
PRIMER_LEFT_12=1573,23
PRIMER_RIGHT_12=1766,23
PRIMER_LEFT_12_TM=60.015
PRIMER_RIGHT_12_TM=59.920
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=194
PRIMER_PAIR_PENALTY_13=0.0945
PRIMER_LEFT_13_PENALTY=0.014712
PRIMER_RIGHT_13_PENALTY=0.079770
PRIMER_LEFT_13_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_13_SEQUENCE=TCCTTGATCGTTTACCAAACAGT
PRIMER_LEFT_13=1573,23
PRIMER_RIGHT_13=1761,23
PRIMER_LEFT_13_TM=60.015
PRIMER_RIGHT_13_TM=59.920
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=189
PRIMER_PAIR_PENALTY_14=0.0945
PRIMER_LEFT_14_PENALTY=0.014712
PRIMER_RIGHT_14_PENALTY=0.079770
PRIMER_LEFT_14_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_14_SEQUENCE=ACTCCTTGATCGTTTACCAAACA
PRIMER_LEFT_14=1573,23
PRIMER_RIGHT_14=1763,23
PRIMER_LEFT_14_TM=60.015
PRIMER_RIGHT_14_TM=59.920
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.6000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=191
PRIMER_PAIR_PENALTY_15=0.0945
PRIMER_LEFT_15_PENALTY=0.014712
PRIMER_RIGHT_15_PENALTY=0.079770
PRIMER_LEFT_15_SEQUENCE=TTCATCATCACTGAGTACATGGC
PRIMER_RIGHT_15_SEQUENCE=AACAACTCCTTGATCGTTTACCA
PRIMER_LEFT_15=1573,23
PRIMER_RIGHT_15=1767,23
PRIMER_LEFT_15_TM=60.015
PRIMER_RIGHT_15_TM=59.920
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=195
PRIMER_PAIR_PENALTY_16=0.0961
PRIMER_LEFT_16_PENALTY=0.081865
PRIMER_RIGHT_16_PENALTY=0.014220
PRIMER_LEFT_16_SEQUENCE=TCCCTTCCAGGTTGTATATGATG
PRIMER_RIGHT_16_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT_16=462,23
PRIMER_RIGHT_16=1166,23
PRIMER_LEFT_16_TM=60.082
PRIMER_RIGHT_16_TM=60.014
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=705
PRIMER_PAIR_PENALTY_17=0.0961
PRIMER_LEFT_17_PENALTY=0.081865
PRIMER_RIGHT_17_PENALTY=0.014220
PRIMER_LEFT_17_SEQUENCE=CCCTTCCAGGTTGTATATGATGA
PRIMER_RIGHT_17_SEQUENCE=ACATAATGACGTATCACCCCTTG
PRIMER_LEFT_17=463,23
PRIMER_RIGHT_17=1166,23
PRIMER_LEFT_17_TM=60.082
PRIMER_RIGHT_17_TM=60.014
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=704
PRIMER_PAIR_PENALTY_18=0.0995
PRIMER_LEFT_18_PENALTY=0.081865
PRIMER_RIGHT_18_PENALTY=0.017590
PRIMER_LEFT_18_SEQUENCE=CCCTTCCAGGTTGTATATGATGA
PRIMER_RIGHT_18_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_18=463,23
PRIMER_RIGHT_18=911,23
PRIMER_LEFT_18_TM=60.082
PRIMER_RIGHT_18_TM=60.018
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=449
PRIMER_PAIR_PENALTY_19=0.0995
PRIMER_LEFT_19_PENALTY=0.081865
PRIMER_RIGHT_19_PENALTY=0.017590
PRIMER_LEFT_19_SEQUENCE=TCCCTTCCAGGTTGTATATGATG
PRIMER_RIGHT_19_SEQUENCE=GGTAAGTTGCTTTCCTCCAAGAT
PRIMER_LEFT_19=462,23
PRIMER_RIGHT_19=911,23
PRIMER_LEFT_19_TM=60.082
PRIMER_RIGHT_19_TM=60.018
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=450
=