PRIMER_SEQUENCE_ID=NM_000215
SEQUENCE=CCCTCTGACCAGGACTGAGGGGCTTTTTCTCTCTGTGCCCCAGGCAAGTTGCACTCATTATGGAATTCCGGCGGCCCGCTAGGCAAGTTGCACTCATGGCACCTCCAAGTGAAGAGACGCCCCTGATCCCTCAGCGTTCATGCAGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCGGGCCCCGGGGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCACTTGGCTGAGGACCTGTGCGTGCAGGCTGCCAAGGCCAGCGGCATCCTGCCTGTGTACCACTCCCTCTTTGCTCTGGCCACGGAGGACCTGTCCTGCTGGTTCCCCCCGAGCCACATCTTCTCCGTGGAGGATGCCAGCACCCAAGTCCTGCTGTACAGGATTCGCTTTTACTTCCCCAATTGGTTTGGGCTGGAGAAGTGCCACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGGGCGCCTCCCCGTGGGCCTCAGTCTCAAGGAGCAGGGTGAGTGTCTCAGCCTGGCCGTGTTGGACCTGGCCCGGATGGCGCGAGAGCAGGCCCAGCGGCCGGGAGAGCTGCTGAAGACTGTCAGCTACAAGGCCTGCCTACCCCCAAGCCTGCGCGACCTGATCCAGGGCCTGAGCTTCGTGACGCGGAGGGCTATTCGGAGGACGGTGCGCAGAGCCCTGCCGCGCGTGGCCGCCTGCCAGGCAGACCGGCACTCGCTCATGGCCAAGTACATCATGGACCTGGAGCGGCTGGATCCAGCCGGGGCCGCCGAGACCTTCCACGTGGGCCTCCCTGGGGCCCTTGGTGGCCACGACGGGCTGGGGCTGCTCCGCGTGGCTGGTGACGGCGGCATCGCCTGGACCCAGGGAGAACAGGAGGTCCTCCAGCCCTTCTGCGACTTTCCAGAAATCGTAGACATTAGCATCAAGCAGGCCCCGCGCGTTGGCCCGGCCGGAGAGCACCGCCTGGTCACTGTTACCAGGACAGACAACCAGATTTTAGAGGCCGAGTTCCCAGGGCTGCCCGAGGCTCTGTCGTTCGTGGCGCTCGTGGACGGCTACTTCCGGCTGACCACGGACTCCCAGCACTTCTTCTGCAAGGAGGTGGCACCGCCGAGGCTGCTGGAGGAAGTGGCCGAGCAGTGCCACGGCCCCATCACTCTGGACTTTGCCATCAACAAGCTCAAGACTGGGGGCTCACGTCCTGGCTCCTATGTTCTCCGCCGCAGCCCCCAGGACTTTGACAGCTTCCTCCTCACTGTCTGTGTCCAGAACCCCCTTGGTCCTGATTATAAGGGCTGCCTCATCCGGCGCAGCCCCACAGGAACCTTCCTTCTGGTTGGCCTCAGCCGACCCCACAGCAGTCTTCGAGAGCTCCTGGCAACCTGCTGGGATGGGGGGCTGCACGTAGATGGGGTGGCAGTGACCCTCACTTCCTGCTGTATCCCCAGACCCAAAGAAAAGTCCAACCTGATCGTGGTCCAGAGAGGTCACAGCCCACCCACATCATCCTTGGTTCAGCCCCAATCCCAATACCAGCTGAGTCAGATGACATTTCACAAGATCCCTGCTGACAGCCTGGAGTGGCATGAGAACCTGGGCCATGGGTCCTTCACCAAGATTTACCGGGGCTGTCGCCATGAGGTGGTGGATGGGGAGGCCCGAAAGACAGAGGTGCTGCTGAAGGTCATGGATGCCAAGCACAAGAACTGCATGGAGTCATTCCTGGAAGCAGCGAGCTTGATGAGCCAAGTGTCGTACCGGCATCTCGTGCTGCTCCACGGCGTGTGCATGGCTGGAGACAGCACCATGGTGCAGGAATTTGTACACCTGGGGGCCATAGACATGTATCTGCGAAAACGTGGCCACCTGGTGCCAGCCAGCTGGAAGCTGCAGGTGGTCAAACAGCTGGCCTACGCCCTCAACTATCTGGAGGACAAAGGCCTGCCCCATGGCAATGTCTCTGCCCGGAAGGTGCTCCTGGCTCGGGAGGGGGCTGATGGGAGCCCGCCCTTCATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGATGCTCACCGACAGGATCCCCTGGGTGGCCCCCGAGTGTCTCCGGGAGGCGCAGACACTTAGCTTGGAAGCTGACAAGTGGGGCTTCGGCGCCACGGTCTGGGAAGTGTTTAGTGGCGTCACCATGCCCATCAGTGCCCTGGATCCTGCTAAGAAACTCCAATTTTATGAGGACCGGCAGCAGCTGCCGGCCCCCAAGTGGACAGAGCTGGCCCTGCTGATTCAACAGTGCATGGCCTATGAGCCGGTCCAGAGGCCCTCCTTCCGAGCCGTCATTCGTGACCTCAATAGCCTCATCTCTTCAGACTATGAGCTCCTCTCAGACCCCACACCTGGTGCCCTGGCACCTCGTGATGGGCTGTGGAATGGTGCCCAGCTCTATGCCTGCCAAGACCCCACGATCTTCGAGGAGAGACACCTCAAGTACATCTCACAGCTGGGCAAGGGCAACTTTGGCAGCGTGGAGCTGTGCCGCTATGACCCGCTAGCCCACAATACAGGTGCCCTGGTGGCCGTGAAACAGCTGCAGCACAGCGGGCCAGACCAGCAGAGGGACTTTCAGCGGGAGATTCAGATCCTCAAAGCACTGCACAGTGATTTCATTGTCAAGTATCGTGGTGTCAGCTATGGCCCGGGCCGGCCAGAGCTGCGGCTGGTCATGGAGTACCTGCCCAGCGGCTGCTTGCGCGACTTCCTGCAGCGGCACCGCGCGCGCCTCGATGCCAGCCGCCTCCTTCTCTATTCCTCGCAGATCTGCAAGGGCATGGAGTACCTGGGCTCCCGCCGCTGCGTGCACCGCGACCTGGCCGCCCGAAACATCCTCGTGGAGAGCGAGGCACACGTCAAGATCGCTGACTTCGGCCTAGCTAAGCTGCTGCCGCTTGACAAAGACTACTACGTGGTCCGCGAGCCAGGCCAGAGCCCCATTTTCTGGTATGCCCCCGAATCCCTCTCGGACAACATCTTCTCTCGCCAGTCAGACGTCTGGAGCTTCGGGGTCGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTGCAGCCCCTCGGCCGAGTTCCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTGCCGCCTCTTGGAACTGCTGGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGCCTGCCCTGCTGAGGTTCACGAGCTCATGAAGCTGTGCTGGGCCCCTAGCCCACAGGACCGGCCATCATTCAGCGCCCTGGGCCCCCAGCTGGACATGCTGTGGAGCGGAAGCCGGGGGTGTGAGACTCATGCCTTCACTGCTCACCCAGAGGGCAAACACCACTCCCTGTCCTTTTCATAGCTCCTGCCCGCAGACCTCTGGATTAGGTCTCTGTTGACTGGCTGTGTGACCTTAGGCCCGGAGCTGCCCCTCTCTGGGCCTCAGAGGCCTTATGAGGGTCCTCTACTTCAGGAACACCCCCATGACATTGCATTTGGGGGGGCTCCCGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGTTAAGGTTCAAGACAGATGGGCATATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCAAAGAAGCAAGGAACCAAATTTAAGACTCTCGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTTTCCTTTTCTCGTCTCTCTCTTTTTATTTTTTTTATTTTTATTTTTATTTTTGAGACAGAGCCTCGCTCGTTACCCAGGGTGGAGTGCAGTGGTAGCGATCTCGGCTCACAGTGCAACCTCTGCTTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGTGCACCACCACACCCGGCTAATTTTTTTTATTTTTAATAGAGATGAGGTTTCACCATGATGGCCAGGCTGATCTCGAACTCCTAACCTCAAGTGATCCTCCCACC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 46984, GC content failed 2171, low tm 9773, high tm 26596, high any compl 9, high end compl 30, high repeat similarity 27, long poly-x seq 87, ok 8291
PRIMER_RIGHT_EXPLAIN=considered 46900, GC content failed 2299, low tm 9880, high tm 26272, high any compl 5, high end compl 7, high repeat similarity 20, long poly-x seq 97, ok 8320
PRIMER_PAIR_EXPLAIN=considered 823, unacceptable product size 769, high end compl 6, ok 48
PRIMER_PAIR_PENALTY=0.1307
PRIMER_LEFT_PENALTY=0.087593
PRIMER_RIGHT_PENALTY=0.043085
PRIMER_LEFT_SEQUENCE=TCATTGTCAAGTATCGTGGTGTC
PRIMER_RIGHT_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT=2739,23
PRIMER_RIGHT=3162,23
PRIMER_LEFT_TM=59.912
PRIMER_RIGHT_TM=59.957
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=424
PRIMER_PAIR_PENALTY_1=0.1310
PRIMER_LEFT_1_PENALTY=0.087907
PRIMER_RIGHT_1_PENALTY=0.043085
PRIMER_LEFT_1_SEQUENCE=TTTCATTGTCAAGTATCGTGGTG
PRIMER_RIGHT_1_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT_1=2737,23
PRIMER_RIGHT_1=3162,23
PRIMER_LEFT_1_TM=59.912
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=426
PRIMER_PAIR_PENALTY_2=0.1454
PRIMER_LEFT_2_PENALTY=0.102347
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=GACAAAGACTACTACGTGGTCCG
PRIMER_RIGHT_2_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT_2=3023,23
PRIMER_RIGHT_2=3162,23
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.1988
PRIMER_LEFT_3_PENALTY=0.087593
PRIMER_RIGHT_3_PENALTY=0.111172
PRIMER_LEFT_3_SEQUENCE=TCATTGTCAAGTATCGTGGTGTC
PRIMER_RIGHT_3_SEQUENCE=ACGTAGTAGTCTTTGTCAAGCGG
PRIMER_LEFT_3=2739,23
PRIMER_RIGHT_3=3039,23
PRIMER_LEFT_3_TM=59.912
PRIMER_RIGHT_3_TM=59.889
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=301
PRIMER_PAIR_PENALTY_4=0.1991
PRIMER_LEFT_4_PENALTY=0.087907
PRIMER_RIGHT_4_PENALTY=0.111172
PRIMER_LEFT_4_SEQUENCE=TTTCATTGTCAAGTATCGTGGTG
PRIMER_RIGHT_4_SEQUENCE=ACGTAGTAGTCTTTGTCAAGCGG
PRIMER_LEFT_4=2737,23
PRIMER_RIGHT_4=3039,23
PRIMER_LEFT_4_TM=59.912
PRIMER_RIGHT_4_TM=59.889
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=303
PRIMER_PAIR_PENALTY_5=0.3062
PRIMER_LEFT_5_PENALTY=0.043085
PRIMER_RIGHT_5_PENALTY=0.263142
PRIMER_LEFT_5_SEQUENCE=TCCTGTACGAGCTCTTCACCTAC
PRIMER_RIGHT_5_SEQUENCE=ACATATGCCCATCTGTCTTGAAC
PRIMER_LEFT_5=3141,23
PRIMER_RIGHT_5=3680,23
PRIMER_LEFT_5_TM=59.957
PRIMER_RIGHT_5_TM=60.263
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=540
PRIMER_PAIR_PENALTY_6=0.3062
PRIMER_LEFT_6_PENALTY=0.043085
PRIMER_RIGHT_6_PENALTY=0.263142
PRIMER_LEFT_6_SEQUENCE=GTCCTGTACGAGCTCTTCACCTA
PRIMER_RIGHT_6_SEQUENCE=ACATATGCCCATCTGTCTTGAAC
PRIMER_LEFT_6=3140,23
PRIMER_RIGHT_6=3680,23
PRIMER_LEFT_6_TM=59.957
PRIMER_RIGHT_6_TM=60.263
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=541
PRIMER_PAIR_PENALTY_7=0.3359
PRIMER_LEFT_7_PENALTY=0.292834
PRIMER_RIGHT_7_PENALTY=0.043085
PRIMER_LEFT_7_SEQUENCE=CAAGTATCGTGGTGTCAGCTATG
PRIMER_RIGHT_7_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT_7=2746,23
PRIMER_RIGHT_7=3162,23
PRIMER_LEFT_7_TM=59.707
PRIMER_RIGHT_7_TM=59.957
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.9000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=417
PRIMER_PAIR_PENALTY_8=0.3507
PRIMER_LEFT_8_PENALTY=0.087593
PRIMER_RIGHT_8_PENALTY=0.263142
PRIMER_LEFT_8_SEQUENCE=TCATTGTCAAGTATCGTGGTGTC
PRIMER_RIGHT_8_SEQUENCE=ACATATGCCCATCTGTCTTGAAC
PRIMER_LEFT_8=2739,23
PRIMER_RIGHT_8=3680,23
PRIMER_LEFT_8_TM=59.912
PRIMER_RIGHT_8_TM=60.263
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=942
PRIMER_PAIR_PENALTY_9=0.3510
PRIMER_LEFT_9_PENALTY=0.087907
PRIMER_RIGHT_9_PENALTY=0.263142
PRIMER_LEFT_9_SEQUENCE=TTTCATTGTCAAGTATCGTGGTG
PRIMER_RIGHT_9_SEQUENCE=ACATATGCCCATCTGTCTTGAAC
PRIMER_LEFT_9=2737,23
PRIMER_RIGHT_9=3680,23
PRIMER_LEFT_9_TM=59.912
PRIMER_RIGHT_9_TM=60.263
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=944
PRIMER_PAIR_PENALTY_10=0.3655
PRIMER_LEFT_10_PENALTY=0.102347
PRIMER_RIGHT_10_PENALTY=0.263142
PRIMER_LEFT_10_SEQUENCE=GACAAAGACTACTACGTGGTCCG
PRIMER_RIGHT_10_SEQUENCE=ACATATGCCCATCTGTCTTGAAC
PRIMER_LEFT_10=3023,23
PRIMER_RIGHT_10=3680,23
PRIMER_LEFT_10_TM=60.102
PRIMER_RIGHT_10_TM=60.263
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.6000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=658
PRIMER_PAIR_PENALTY_11=0.3973
PRIMER_LEFT_11_PENALTY=0.354187
PRIMER_RIGHT_11_PENALTY=0.043085
PRIMER_LEFT_11_SEQUENCE=CTCCTTCTCTATTCCTCGCAGAT
PRIMER_RIGHT_11_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT_11=2870,23
PRIMER_RIGHT_11=3162,23
PRIMER_LEFT_11_TM=60.354
PRIMER_RIGHT_11_TM=59.957
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=293
PRIMER_PAIR_PENALTY_12=0.4040
PRIMER_LEFT_12_PENALTY=0.292834
PRIMER_RIGHT_12_PENALTY=0.111172
PRIMER_LEFT_12_SEQUENCE=CAAGTATCGTGGTGTCAGCTATG
PRIMER_RIGHT_12_SEQUENCE=ACGTAGTAGTCTTTGTCAAGCGG
PRIMER_LEFT_12=2746,23
PRIMER_RIGHT_12=3039,23
PRIMER_LEFT_12_TM=59.707
PRIMER_RIGHT_12_TM=59.889
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=11.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=294
PRIMER_PAIR_PENALTY_13=0.4429
PRIMER_LEFT_13_PENALTY=0.087593
PRIMER_RIGHT_13_PENALTY=0.355258
PRIMER_LEFT_13_SEQUENCE=TCATTGTCAAGTATCGTGGTGTC
PRIMER_RIGHT_13_SEQUENCE=CAGATCTGCGAGGAATAGAGAAG
PRIMER_LEFT_13=2739,23
PRIMER_RIGHT_13=2895,23
PRIMER_LEFT_13_TM=59.912
PRIMER_RIGHT_13_TM=59.645
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=157
PRIMER_PAIR_PENALTY_14=0.4432
PRIMER_LEFT_14_PENALTY=0.087907
PRIMER_RIGHT_14_PENALTY=0.355258
PRIMER_LEFT_14_SEQUENCE=TTTCATTGTCAAGTATCGTGGTG
PRIMER_RIGHT_14_SEQUENCE=CAGATCTGCGAGGAATAGAGAAG
PRIMER_LEFT_14=2737,23
PRIMER_RIGHT_14=2895,23
PRIMER_LEFT_14_TM=59.912
PRIMER_RIGHT_14_TM=59.645
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=159
PRIMER_PAIR_PENALTY_15=0.4654
PRIMER_LEFT_15_PENALTY=0.354187
PRIMER_RIGHT_15_PENALTY=0.111172
PRIMER_LEFT_15_SEQUENCE=CTCCTTCTCTATTCCTCGCAGAT
PRIMER_RIGHT_15_SEQUENCE=ACGTAGTAGTCTTTGTCAAGCGG
PRIMER_LEFT_15=2870,23
PRIMER_RIGHT_15=3039,23
PRIMER_LEFT_15_TM=60.354
PRIMER_RIGHT_15_TM=59.889
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=11.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=170
PRIMER_PAIR_PENALTY_16=0.4705
PRIMER_LEFT_16_PENALTY=0.085505
PRIMER_RIGHT_16_PENALTY=0.385016
PRIMER_LEFT_16_SEQUENCE=TACCAGGACAGACAACCAGATTT
PRIMER_RIGHT_16_SEQUENCE=ACGATCAGGTTGGACTTTTCTTT
PRIMER_LEFT_16=1054,23
PRIMER_RIGHT_16=1554,23
PRIMER_LEFT_16_TM=59.914
PRIMER_RIGHT_16_TM=60.385
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=501
PRIMER_PAIR_PENALTY_17=0.4814
PRIMER_LEFT_17_PENALTY=0.438317
PRIMER_RIGHT_17_PENALTY=0.043085
PRIMER_LEFT_17_SEQUENCE=AAGTATCGTGGTGTCAGCTATGG
PRIMER_RIGHT_17_SEQUENCE=TAGGTGAAGAGCTCGTACAGGAC
PRIMER_LEFT_17=2747,23
PRIMER_RIGHT_17=3162,23
PRIMER_LEFT_17_TM=60.438
PRIMER_RIGHT_17_TM=59.957
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.4000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=416
PRIMER_PAIR_PENALTY_18=0.5100
PRIMER_LEFT_18_PENALTY=0.043085
PRIMER_RIGHT_18_PENALTY=0.466925
PRIMER_LEFT_18_SEQUENCE=TCCTGTACGAGCTCTTCACCTAC
PRIMER_RIGHT_18_SEQUENCE=GCGAGAGTCTTAAATTTGGTTCC
PRIMER_LEFT_18=3141,23
PRIMER_RIGHT_18=3741,23
PRIMER_LEFT_18_TM=59.957
PRIMER_RIGHT_18_TM=60.467
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=601
PRIMER_PAIR_PENALTY_19=0.5100
PRIMER_LEFT_19_PENALTY=0.043085
PRIMER_RIGHT_19_PENALTY=0.466925
PRIMER_LEFT_19_SEQUENCE=GTCCTGTACGAGCTCTTCACCTA
PRIMER_RIGHT_19_SEQUENCE=GCGAGAGTCTTAAATTTGGTTCC
PRIMER_LEFT_19=3140,23
PRIMER_RIGHT_19=3741,23
PRIMER_LEFT_19_TM=59.957
PRIMER_RIGHT_19_TM=60.467
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=602
=