PRIMER_SEQUENCE_ID=NM_000459
SEQUENCE=CTTCTGTGCTGTTCCTTCTTGCCTCTAACTTGTAAACAAGACGTACTAGGACGATGCTAATGGAAAGTCACAAACCGCTGGGTTTTTGAAAGGATCCTTGGGACCTCATGCACATTTGTGGAAACTGGATGGAGAGATTTGGGGAAGCATGGACTCTTTAGCCAGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTCTGGAACTGTGGAAGGTGCCATGGACTTGATCTTGATCAATTCCCTACCTCTTGTATCTGATGCTGAAACATCTCTCACCTGCATTGCCTCTGGGTGGCGCCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAGGATCCGCTGGAAGTTACTCAAGATGTGACCAGAGAATGGGCTAAAAAAGTTGTTTGGAAGAGAGAAAAGGCTAGTAAGATCAATGGTGCTTATTTCTGTGAAGGGCGAGTTCGAGGAGAGGCAATCAGGATACGAACCATGAAGATGCGTCAACAAGCTTCCTTCCTACCAGCTACTTTAACTATGACTGTGGACAAGGGAGATAACGTGAACATATCTTTCAAAAAGGTATTGATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGTTCCTTCATCCATTCAGTGCCCCGGCATGAAGTACCTGATATTCTAGAAGTACACCTGCCTCATGCTCAGCCCCAGGATGCTGGAGTGTACTCGGCCAGGTATATAGGAGGAAACCTCTTCACCTCGGCCTTCACCAGGCTGATAGTCCGGAGATGTGAAGCCCAGAAGTGGGGACCTGAATGCAACCATCTCTGTACTGCTTGTATGAACAATGGTGTCTGCCATGAAGATACTGGAGAATGCATTTGCCCTCCTGGGTTTATGGGAAGGACGTGTGAGAAGGCTTGTGAACTGCACACGTTTGGCAGAACTTGTAAAGAAAGGTGCAGTGGACAAGAGGGATGCAAGTCTTATGTGTTCTGTCTCCCTGACCCCTATGGGTGTTCCTGTGCCACAGGCTGGAAGGGTCTGCAGTGCAATGAAGCATGCCACCCTGGTTTTTACGGGCCAGATTGTAAGCTTAGGTGCAGCTGCAACAATGGGGAGATGTGTGATCGCTTCCAAGGATGTCTCTGCTCTCCAGGATGGCAGGGGCTCCAGTGTGAGAGAGAAGGCATACCGAGGATGACCCCAAAGATAGTGGATTTGCCAGATCATATAGAAGTAAACAGTGGTAAATTTAATCCCATTTGCAAAGCTTCTGGCTGGCCGCTACCTACTAATGAAGAAATGACCCTGGTGAAGCCGGATGGGACAGTGCTCCATCCAAAAGACTTTAACCATACGGATCATTTCTCAGTAGCCATATTCACCATCCACCGGATCCTCCCCCCTGACTCAGGAGTTTGGGTCTGCAGTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCCTTCAACATTTCTGTTAAAGTTCTTCCAAAGCCCCTGAATGCCCCAAACGTGATTGACACTGGACATAACTTTGCTGTCATCAACATCAGCTCTGAGCCTTACTTTGGGGATGGACCAATCAAATCCAAGAAGCTTCTATACAAACCCGTTAATCACTATGAGGCTTGGCAACATATTCAAGTGACAAATGAGATTGTTACACTCAACTATTTGGAACCTCGGACAGAATATGAACTCTGTGTGCAACTGGTCCGTCGTGGAGAGGGTGGGGAAGGGCATCCTGGACCTGTGAGACGCTTCACAACAGCTTCTATCGGACTCCCTCCTCCAAGAGGTCTAAATCTCCTGCCTAAAAGTCAGACCACTCTAAATTTGACCTGGCAACCAATATTTCCAAGCTCGGAAGATGACTTTTATGTTGAAGTGGAGAGAAGGTCTGTGCAAAAAAGTGATCAGCAGAATATTAAAGTTCCAGGCAACTTGACTTCGGTGCTACTTAACAACTTACATCCCAGGGAGCAGTACGTGGTCCGAGCTAGAGTCAACACCAAGGCCCAGGGGGAATGGAGTGAAGATCTCACTGCTTGGACCCTTAGTGACATTCTTCCTCCTCAACCAGAAAACATCAAGATTTCCAACATTACACACTCCTCGGCTGTGATTTCTTGGACAATATTGGATGGCTATTCTATTTCTTCTATTACTATCCGTTACAAGGTTCAAGGCAAGAATGAAGACCAGCACGTTGATGTGAAGATAAAGAATGCCACCATCATTCAGTATCAGCTCAAGGGCCTAGAGCCTGAAACAGCATACCAGGTGGACATTTTTGCAGAGAACAACATAGGGTCAAGCAACCCAGCCTTTTCTCATGAACTGGTGACCCTCCCAGAATCTCAAGCACCAGCGGACCTCGGAGGGGGGAAGATGCTGCTTATAGCCATCCTTGGCTCTGCTGGAATGACCTGCCTGACTGTGCTGTTGGCCTTTCTGATCATATTGCAATTGAAGAGGGCAAATGTGCAAAGGAGAATGGCCCAAGCCTTCCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAACTCAGGGACTCTGGCCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTACAATTTATCCAGTGCTTGACTGGAATGACATCAAATTTCAAGATGTGATTGGGGAGGGCAATTTTGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGGTTACGGATGGATGCTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGATGATCACAGGGACTTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGACACCATCCAAACATCATCAATCTCTTAGGAGCATGTGAACATCGAGGCTACTTGTACCTGGCCATTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCTTCGCAAGAGCCGTGTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATAGCACCGCGTCCACACTGTCCTCCCAGCAGCTCCTTCACTTCGCTGCCGACGTGGCCCGGGGCATGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGATCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAGCAGATTTTGGATTGTCCCGAGGTCAAGAGGTGTACGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGTGTACACAACCAACAGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGATTGTTAGCTTAGGAGGCACACCCTACTGCGGGATGACTTGTGCAGAACTCTACGAGAAGCTGCCCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATGAGAGGCCATCATTTGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAGGAGCGAAAGACCTACGTGAATACCACGCTTTATGAGAAGTTTACTTATGCAGGAATTGACTGTTCTGCTGAAGAAGCGGCCTAGGACAGAACATCTGTATACCCTCTGTTTCCCTTTCACTGGCATGGGAGACCCTTGACAACTGCTGAGAAAACATGCCTCTGCCAAAGGATGTGATATATAAGTGTACATATGTGCTGGAATTCTAACAAGTCATAGGTTAATATTTAAGACACTGAAAAATCTAAGTGATATAAATCAGATTCTTCTCTCTCATTTTATCCCTCACCTGTAGCATGCCAGTCCCGTTTCATTTAGTCATGTGACCACTCTGTCTTGTGTTTCCACAGCCTGCAAGTTCAGTCCAGGATGCTAACATCTAAAAATAGACTTAAATCTCATTGCTTACAAGCCTAAGAATCTTTAGAGAAGTATACATAAGTTTAGGATAAAATAATGGGATTTTCTTTTCTTTTCTCTGGTAATATTGACTTGTATATTTTAAGAAATAACAGAAAGCCTGGGTGACATTTGGGAGACATGTGACATTTATATATTGAATTAATATCCCTACATGTATTGCACATTGTAAAAAGTTTTAGTTTTGATGAGTTGTGAGTTTACCTTGTATACTGTAGGCACACTTTGCACTGATATATCATGAGTGAATAAATGTCTTGCCTACTCAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47808, GC content failed 700, low tm 22586, high tm 13637, high any compl 5, high end compl 28, high repeat similarity 23, long poly-x seq 84, ok 10745
PRIMER_RIGHT_EXPLAIN=considered 47749, GC content failed 711, low tm 22771, high tm 13465, high any compl 2, high end compl 6, high repeat similarity 14, long poly-x seq 99, ok 10681
PRIMER_PAIR_EXPLAIN=considered 589, unacceptable product size 528, high end compl 5, ok 56
PRIMER_PAIR_PENALTY=0.0230
PRIMER_LEFT_PENALTY=0.008825
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=CCCTTAGTGACATTCTTCCTCCT
PRIMER_RIGHT_SEQUENCE=TTCCAGTCAAGCACTGGATAAAT
PRIMER_LEFT=2045,23
PRIMER_RIGHT=2612,23
PRIMER_LEFT_TM=60.009
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=568
PRIMER_PAIR_PENALTY_1=0.0230
PRIMER_LEFT_1_PENALTY=0.008825
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CCCTTAGTGACATTCTTCCTCCT
PRIMER_RIGHT_1_SEQUENCE=ATTCCAGTCAAGCACTGGATAAA
PRIMER_LEFT_1=2045,23
PRIMER_RIGHT_1=2613,23
PRIMER_LEFT_1_TM=60.009
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER7A Nonautonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=569
PRIMER_PAIR_PENALTY_2=0.0443
PRIMER_LEFT_2_PENALTY=0.035468
PRIMER_RIGHT_2_PENALTY=0.008825
PRIMER_LEFT_2_SEQUENCE=ATGACCCCAAAGATAGTGGATTT
PRIMER_RIGHT_2_SEQUENCE=AGGAGGAAGAATGTCACTAAGGG
PRIMER_LEFT_2=1189,23
PRIMER_RIGHT_2=2067,23
PRIMER_LEFT_2_TM=59.965
PRIMER_RIGHT_2_TM=60.009
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=19.00, MER21 a consensus
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=879
PRIMER_PAIR_PENALTY_3=0.0572
PRIMER_LEFT_3_PENALTY=0.008825
PRIMER_RIGHT_3_PENALTY=0.048411
PRIMER_LEFT_3_SEQUENCE=CCCTTAGTGACATTCTTCCTCCT
PRIMER_RIGHT_3_SEQUENCE=ACCCTATGTTGTTCTCTGCAAAA
PRIMER_LEFT_3=2045,23
PRIMER_RIGHT_3=2305,23
PRIMER_LEFT_3_TM=60.009
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, PAB Human pseudoautosomal boundary-like sequence.
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.0643
PRIMER_LEFT_4_PENALTY=0.008825
PRIMER_RIGHT_4_PENALTY=0.055462
PRIMER_LEFT_4_SEQUENCE=CCCTTAGTGACATTCTTCCTCCT
PRIMER_RIGHT_4_SEQUENCE=GAATAGCCATCCAATATTGTCCA
PRIMER_LEFT_4=2045,23
PRIMER_RIGHT_4=2144,23
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=60.055
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=100
PRIMER_PAIR_PENALTY_5=0.0909
PRIMER_LEFT_5_PENALTY=0.035468
PRIMER_RIGHT_5_PENALTY=0.055462
PRIMER_LEFT_5_SEQUENCE=ATGACCCCAAAGATAGTGGATTT
PRIMER_RIGHT_5_SEQUENCE=GAATAGCCATCCAATATTGTCCA
PRIMER_LEFT_5=1189,23
PRIMER_RIGHT_5=2144,23
PRIMER_LEFT_5_TM=59.965
PRIMER_RIGHT_5_TM=60.055
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=956
PRIMER_PAIR_PENALTY_6=0.1028
PRIMER_LEFT_6_PENALTY=0.074695
PRIMER_RIGHT_6_PENALTY=0.028128
PRIMER_LEFT_6_SEQUENCE=ATGTGAACATCGAGGCTACTTGT
PRIMER_RIGHT_6_SEQUENCE=TCTTTCGCTCCTCTAACATTCTG
PRIMER_LEFT_6=2820,23
PRIMER_RIGHT_6=3448,23
PRIMER_LEFT_6_TM=60.075
PRIMER_RIGHT_6_TM=60.028
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=629
PRIMER_PAIR_PENALTY_7=0.1226
PRIMER_LEFT_7_PENALTY=0.034674
PRIMER_RIGHT_7_PENALTY=0.087907
PRIMER_LEFT_7_SEQUENCE=ACCTACGTGAATACCACGCTTTA
PRIMER_RIGHT_7_SEQUENCE=TGGTCACATGACTAAATGAAACG
PRIMER_LEFT_7=3448,23
PRIMER_RIGHT_7=3767,23
PRIMER_LEFT_7_TM=59.965
PRIMER_RIGHT_7_TM=59.912
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=10.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=320
PRIMER_PAIR_PENALTY_8=0.1267
PRIMER_LEFT_8_PENALTY=0.112479
PRIMER_RIGHT_8_PENALTY=0.014220
PRIMER_LEFT_8_SEQUENCE=ACCCTTAGTGACATTCTTCCTCC
PRIMER_RIGHT_8_SEQUENCE=TTCCAGTCAAGCACTGGATAAAT
PRIMER_LEFT_8=2044,23
PRIMER_RIGHT_8=2612,23
PRIMER_LEFT_8_TM=59.888
PRIMER_RIGHT_8_TM=60.014
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=6.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=569
PRIMER_PAIR_PENALTY_9=0.1267
PRIMER_LEFT_9_PENALTY=0.112479
PRIMER_RIGHT_9_PENALTY=0.014220
PRIMER_LEFT_9_SEQUENCE=ACCCTTAGTGACATTCTTCCTCC
PRIMER_RIGHT_9_SEQUENCE=ATTCCAGTCAAGCACTGGATAAA
PRIMER_LEFT_9=2044,23
PRIMER_RIGHT_9=2613,23
PRIMER_LEFT_9_TM=59.888
PRIMER_RIGHT_9_TM=60.014
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER7A Nonautonomous DNA transposon
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=6.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=570
PRIMER_PAIR_PENALTY_10=0.1267
PRIMER_LEFT_10_PENALTY=0.112479
PRIMER_RIGHT_10_PENALTY=0.014220
PRIMER_LEFT_10_SEQUENCE=GGACCCTTAGTGACATTCTTCCT
PRIMER_RIGHT_10_SEQUENCE=TTCCAGTCAAGCACTGGATAAAT
PRIMER_LEFT_10=2042,23
PRIMER_RIGHT_10=2612,23
PRIMER_LEFT_10_TM=59.888
PRIMER_RIGHT_10_TM=60.014
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=6.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=571
PRIMER_PAIR_PENALTY_11=0.1267
PRIMER_LEFT_11_PENALTY=0.112479
PRIMER_RIGHT_11_PENALTY=0.014220
PRIMER_LEFT_11_SEQUENCE=GGACCCTTAGTGACATTCTTCCT
PRIMER_RIGHT_11_SEQUENCE=ATTCCAGTCAAGCACTGGATAAA
PRIMER_LEFT_11=2042,23
PRIMER_RIGHT_11=2613,23
PRIMER_LEFT_11_TM=59.888
PRIMER_RIGHT_11_TM=60.014
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=8.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MER7A Nonautonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=6.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=572
PRIMER_PAIR_PENALTY_12=0.1370
PRIMER_LEFT_12_PENALTY=0.088604
PRIMER_RIGHT_12_PENALTY=0.048411
PRIMER_LEFT_12_SEQUENCE=CAACATCAGCTCTGAGCCTTACT
PRIMER_RIGHT_12_SEQUENCE=ACCCTATGTTGTTCTCTGCAAAA
PRIMER_LEFT_12=1536,23
PRIMER_RIGHT_12=2305,23
PRIMER_LEFT_12_TM=60.089
PRIMER_RIGHT_12_TM=60.048
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=770
PRIMER_PAIR_PENALTY_13=0.1441
PRIMER_LEFT_13_PENALTY=0.088604
PRIMER_RIGHT_13_PENALTY=0.055462
PRIMER_LEFT_13_SEQUENCE=CAACATCAGCTCTGAGCCTTACT
PRIMER_RIGHT_13_SEQUENCE=GAATAGCCATCCAATATTGTCCA
PRIMER_LEFT_13=1536,23
PRIMER_RIGHT_13=2144,23
PRIMER_LEFT_13_TM=60.089
PRIMER_RIGHT_13_TM=60.055
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=609
PRIMER_PAIR_PENALTY_14=0.1479
PRIMER_LEFT_14_PENALTY=0.035468
PRIMER_RIGHT_14_PENALTY=0.112479
PRIMER_LEFT_14_SEQUENCE=ATGACCCCAAAGATAGTGGATTT
PRIMER_RIGHT_14_SEQUENCE=GGAGGAAGAATGTCACTAAGGGT
PRIMER_LEFT_14=1189,23
PRIMER_RIGHT_14=2066,23
PRIMER_LEFT_14_TM=59.965
PRIMER_RIGHT_14_TM=59.888
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER21 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=878
PRIMER_PAIR_PENALTY_15=0.1479
PRIMER_LEFT_15_PENALTY=0.035468
PRIMER_RIGHT_15_PENALTY=0.112479
PRIMER_LEFT_15_SEQUENCE=ATGACCCCAAAGATAGTGGATTT
PRIMER_RIGHT_15_SEQUENCE=AGGAAGAATGTCACTAAGGGTCC
PRIMER_LEFT_15=1189,23
PRIMER_RIGHT_15=2064,23
PRIMER_LEFT_15_TM=59.965
PRIMER_RIGHT_15_TM=59.888
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=9.1000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=876
PRIMER_PAIR_PENALTY_16=0.1494
PRIMER_LEFT_16_PENALTY=0.074695
PRIMER_RIGHT_16_PENALTY=0.074695
PRIMER_LEFT_16_SEQUENCE=ATGTGAACATCGAGGCTACTTGT
PRIMER_RIGHT_16_SEQUENCE=GCTTCTTCAGCAGAACAGTCAAT
PRIMER_LEFT_16=2820,23
PRIMER_RIGHT_16=3515,23
PRIMER_LEFT_16_TM=60.075
PRIMER_RIGHT_16_TM=60.075
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=696
PRIMER_PAIR_PENALTY_17=0.1557
PRIMER_LEFT_17_PENALTY=0.008825
PRIMER_RIGHT_17_PENALTY=0.146910
PRIMER_LEFT_17_SEQUENCE=CCCTTAGTGACATTCTTCCTCCT
PRIMER_RIGHT_17_SEQUENCE=TGAGCTGATACTGAATGATGGTG
PRIMER_LEFT_17=2045,23
PRIMER_RIGHT_17=2245,23
PRIMER_LEFT_17_TM=60.009
PRIMER_RIGHT_17_TM=60.147
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=201
PRIMER_PAIR_PENALTY_18=0.1566
PRIMER_LEFT_18_PENALTY=0.035468
PRIMER_RIGHT_18_PENALTY=0.121175
PRIMER_LEFT_18_SEQUENCE=ATGACCCCAAAGATAGTGGATTT
PRIMER_RIGHT_18_SEQUENCE=CACCAGGGTCATTTCTTCATTAG
PRIMER_LEFT_18=1189,23
PRIMER_RIGHT_18=1305,23
PRIMER_LEFT_18_TM=59.965
PRIMER_RIGHT_18_TM=59.879
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=117
PRIMER_PAIR_PENALTY_19=0.1580
PRIMER_LEFT_19_PENALTY=0.149159
PRIMER_RIGHT_19_PENALTY=0.008825
PRIMER_LEFT_19_SEQUENCE=TATTTGGAACCTCGGACAGAATA
PRIMER_RIGHT_19_SEQUENCE=AGGAGGAAGAATGTCACTAAGGG
PRIMER_LEFT_19=1663,23
PRIMER_RIGHT_19=2067,23
PRIMER_LEFT_19_TM=59.851
PRIMER_RIGHT_19_TM=60.009
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=405
=