PRIMER_SEQUENCE_ID=NM_001715
SEQUENCE=CACACAGATGGCACATGGCAGAGCCCAAGCCCTCTCCATGAGATCTGCAGCGTTTGTCCACACGGCAGGGCAAATTACCTTGTAGAGTCAGCATGGAGGTTTGCTAACTCTGCCGGACTTGGTGCAGAAAAAATACCAGCCGTGCTGTTAAACCAAAACCCCAGGAGAAAACTTTCTGGTGACAGAAGGGCATTGTGACCCACGTTGCCCAGGGCCAGGGACAGGGCAGCTGCTCATGGTCCTTCCTCACGAGTTTTCCTGGAGCAGAGGGATACCCCCAAACCCAGGGAGCCCACAGGAGCTTCAGAAGGGCTCGGCTGGAGGGCGGGGCCCCTGTTGCACTTGCTCACTGGGGACTGGGGAGGCTCTGATCGCAGACCGGGGGTGCTGCCACCTCTGTCTGCTGCCGGCAGAAAGCCACAAGCCATGAAAACTGATTGAGATGAGAAGAATTCATCTGGGACTGGCTTTTGCTTTAGGATGGTGTTGGAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCAGGATCTGAAGAGCTATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGCAAAAAGCCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTGAAGGTCAGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCTTACTCCTCCACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGACTACACCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTGAAGGGAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTGCCCAGTAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCACAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGCTCCTTTCTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTCTCCCTGTCTGTGAAGGATGTCACCACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGCTGCCTGGATGAAGGGGGCTACTACATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCCCTGGTGCAGCACTATTCTAAGAAGGGGGATGGTCTATGCCAGAGGCTGACCCTGCCCTGTGTGCGCCCGGCCCCGCAGAATCCCTGGGCCCAGGATGAATGGGAGATCCCCCGGCAGTCTCTCAGGCTGGTCAGGAAACTCGGGTCTGGACAATTCGGCGAAGTCTGGATGGGTTACTACAAAAACAACATGAAGGTGGCCATTAAGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTTCTGGGTGAGGCCAACGTGATGAAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAGCCCATCTACATTGTCACCGAGTACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGATGAAGGGAGCAGATTGTCACTCCCAAGGCTGATTGACATGTCGGCGCAGATTGCTGAAGGGATGGCATACATTGAGCGCATGAATTCCATCCACCGCGACCTGCGGGCGGCCAACATCCTGGTGTCTGAGGCCTTGTGCTGCAAAATTGCTGATTTTGGCTTGGCTCGAATCATCGACAGTGAATACACGGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACAGCCCCGGAAGCCATCCACTTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCGTTTGGAGTCCTCCTGATGGAAGTTGTCACTTATGGGCGGGTGCCATACCCAGGGATGAGCAACCCCGAGGTCATCCGCAACCTGGAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGCCCGCCCGAGCTGTACCGCGGCGTCATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGGCCCACCTTCGAGTTCCTGCAGTCGGTGCTGGAGGACTTCTACACGGCCACCGAGCGGCAGTACGAGCTGCAGCCCTAGCCGGCCGCGCCCGCCTGCGCCCCGTGCCCACCTCTGCGCGGACGACCCCGACTTCCGTGCCATCCCAGACGGGCCGCGAAGGCGGGGTGTCGCCTGTGCCCTTTTCTCAGACCCGGAATCCAGTGGGCAGAGGCAGCTTCGCAGGGGGTCCCCGGACGGACTCCTTCACCGACTGCACCCCCGGGCGAGTTACGCGGCCTCTCTGTGCCGCTTCATTTGTAGAGGGCTGTAACAGTGACCTCGCACGGTCATCCGGAGTACTAAGCCCCAGTAAGGTGTTCAGGACTGGTAAGCGACTGTCATCAAGTAAGGCCCCCGTGCTGGGCACCCCCCGTGCTGGCCGCGTCCCCGCCTCTGCGCCCTGCGTGGACCCCGCCCTGCCCCGCTACAGAAGCCAGACTGGGTCCCGCGGACGCCAGCAGGGGCAGCCCCAGCCTAGGCTGCGCTCCAGCACTGCGGGGCTTTTCTGCAATAAAGTCACGAGCGTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 30093, GC content failed 1757, low tm 7075, high tm 15567, high any compl 6, high end compl 18, high repeat similarity 17, long poly-x seq 53, ok 5600
PRIMER_RIGHT_EXPLAIN=considered 29967, GC content failed 2187, low tm 6921, high tm 15256, high end compl 7, high repeat similarity 6, long poly-x seq 73, ok 5517
PRIMER_PAIR_EXPLAIN=considered 442, unacceptable product size 397, high end compl 2, ok 43
PRIMER_PAIR_PENALTY=0.0622
PRIMER_LEFT_PENALTY=0.049500
PRIMER_RIGHT_PENALTY=0.012719
PRIMER_LEFT_SEQUENCE=CTGGTTGTCTTCAACCACCTTAC
PRIMER_RIGHT_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT=701,23
PRIMER_RIGHT=964,23
PRIMER_LEFT_TM=59.950
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.0622
PRIMER_LEFT_1_PENALTY=0.049500
PRIMER_RIGHT_1_PENALTY=0.012719
PRIMER_LEFT_1_SEQUENCE=TGGTTGTCTTCAACCACCTTACT
PRIMER_RIGHT_1_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT_1=702,23
PRIMER_RIGHT_1=964,23
PRIMER_LEFT_1_TM=59.950
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.1885
PRIMER_LEFT_2_PENALTY=0.175797
PRIMER_RIGHT_2_PENALTY=0.012719
PRIMER_LEFT_2_SEQUENCE=CTTGTAGAGTCAGCATGGAGGTT
PRIMER_RIGHT_2_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT_2=78,23
PRIMER_RIGHT_2=964,23
PRIMER_LEFT_2_TM=59.824
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=887
PRIMER_PAIR_PENALTY_3=0.1911
PRIMER_LEFT_3_PENALTY=0.178426
PRIMER_RIGHT_3_PENALTY=0.012719
PRIMER_LEFT_3_SEQUENCE=TTGTAGAGTCAGCATGGAGGTTT
PRIMER_RIGHT_3_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT_3=79,23
PRIMER_RIGHT_3=964,23
PRIMER_LEFT_3_TM=60.178
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=886
PRIMER_PAIR_PENALTY_4=0.2212
PRIMER_LEFT_4_PENALTY=0.175797
PRIMER_RIGHT_4_PENALTY=0.045432
PRIMER_LEFT_4_SEQUENCE=CTTGTAGAGTCAGCATGGAGGTT
PRIMER_RIGHT_4_SEQUENCE=TCAGATCCTGACACCCTTACAGT
PRIMER_LEFT_4=78,23
PRIMER_RIGHT_4=543,23
PRIMER_LEFT_4_TM=59.824
PRIMER_RIGHT_4_TM=60.045
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=466
PRIMER_PAIR_PENALTY_5=0.2239
PRIMER_LEFT_5_PENALTY=0.178426
PRIMER_RIGHT_5_PENALTY=0.045432
PRIMER_LEFT_5_SEQUENCE=TTGTAGAGTCAGCATGGAGGTTT
PRIMER_RIGHT_5_SEQUENCE=TCAGATCCTGACACCCTTACAGT
PRIMER_LEFT_5=79,23
PRIMER_RIGHT_5=543,23
PRIMER_LEFT_5_TM=60.178
PRIMER_RIGHT_5_TM=60.045
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=465
PRIMER_PAIR_PENALTY_6=0.2296
PRIMER_LEFT_6_PENALTY=0.175797
PRIMER_RIGHT_6_PENALTY=0.053769
PRIMER_LEFT_6_SEQUENCE=CTTGTAGAGTCAGCATGGAGGTT
PRIMER_RIGHT_6_SEQUENCE=GTGGTGTTTCACCATAGCTCTTC
PRIMER_LEFT_6=78,23
PRIMER_RIGHT_6=564,23
PRIMER_LEFT_6_TM=59.824
PRIMER_RIGHT_6_TM=60.054
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER35 a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=487
PRIMER_PAIR_PENALTY_7=0.2322
PRIMER_LEFT_7_PENALTY=0.178426
PRIMER_RIGHT_7_PENALTY=0.053769
PRIMER_LEFT_7_SEQUENCE=TTGTAGAGTCAGCATGGAGGTTT
PRIMER_RIGHT_7_SEQUENCE=GTGGTGTTTCACCATAGCTCTTC
PRIMER_LEFT_7=79,23
PRIMER_RIGHT_7=564,23
PRIMER_LEFT_7_TM=60.178
PRIMER_RIGHT_7_TM=60.054
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER35 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=486
PRIMER_PAIR_PENALTY_8=0.2583
PRIMER_LEFT_8_PENALTY=0.049500
PRIMER_RIGHT_8_PENALTY=0.208756
PRIMER_LEFT_8_SEQUENCE=TGGTTGTCTTCAACCACCTTACT
PRIMER_RIGHT_8_SEQUENCE=CAGCGGATCTTATAGTGCTTGAT
PRIMER_LEFT_8=702,23
PRIMER_RIGHT_8=1122,23
PRIMER_LEFT_8_TM=59.950
PRIMER_RIGHT_8_TM=59.791
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.7000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=421
PRIMER_PAIR_PENALTY_9=0.3060
PRIMER_LEFT_9_PENALTY=0.012719
PRIMER_RIGHT_9_PENALTY=0.293240
PRIMER_LEFT_9_SEQUENCE=TGGAAAGGTGGTTCTTTAGATCA
PRIMER_RIGHT_9_SEQUENCE=CACCTTTGTTGGTTTCACTCTCT
PRIMER_LEFT_9=942,23
PRIMER_RIGHT_9=1055,23
PRIMER_LEFT_9_TM=59.987
PRIMER_RIGHT_9_TM=59.707
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=114
PRIMER_PAIR_PENALTY_10=0.3427
PRIMER_LEFT_10_PENALTY=0.049500
PRIMER_RIGHT_10_PENALTY=0.293240
PRIMER_LEFT_10_SEQUENCE=CTGGTTGTCTTCAACCACCTTAC
PRIMER_RIGHT_10_SEQUENCE=CACCTTTGTTGGTTTCACTCTCT
PRIMER_LEFT_10=701,23
PRIMER_RIGHT_10=1055,23
PRIMER_LEFT_10_TM=59.950
PRIMER_RIGHT_10_TM=59.707
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.7000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=355
PRIMER_PAIR_PENALTY_11=0.3427
PRIMER_LEFT_11_PENALTY=0.049500
PRIMER_RIGHT_11_PENALTY=0.293240
PRIMER_LEFT_11_SEQUENCE=TGGTTGTCTTCAACCACCTTACT
PRIMER_RIGHT_11_SEQUENCE=CACCTTTGTTGGTTTCACTCTCT
PRIMER_LEFT_11=702,23
PRIMER_RIGHT_11=1055,23
PRIMER_LEFT_11_TM=59.950
PRIMER_RIGHT_11_TM=59.707
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=354
PRIMER_PAIR_PENALTY_12=0.3442
PRIMER_LEFT_12_PENALTY=0.331503
PRIMER_RIGHT_12_PENALTY=0.012719
PRIMER_LEFT_12_SEQUENCE=TGTCTTCAACCACCTTACTCCTC
PRIMER_RIGHT_12_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT_12=706,23
PRIMER_RIGHT_12=964,23
PRIMER_LEFT_12_TM=59.668
PRIMER_RIGHT_12_TM=59.987
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=259
PRIMER_PAIR_PENALTY_13=0.4073
PRIMER_LEFT_13_PENALTY=0.394601
PRIMER_RIGHT_13_PENALTY=0.012719
PRIMER_LEFT_13_SEQUENCE=GTCTTCAACCACCTTACTCCTCC
PRIMER_RIGHT_13_SEQUENCE=TGATCTAAAGAACCACCTTTCCA
PRIMER_LEFT_13=707,23
PRIMER_RIGHT_13=964,23
PRIMER_LEFT_13_TM=60.395
PRIMER_RIGHT_13_TM=59.987
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=258
PRIMER_PAIR_PENALTY_14=0.4166
PRIMER_LEFT_14_PENALTY=0.175797
PRIMER_RIGHT_14_PENALTY=0.240778
PRIMER_LEFT_14_SEQUENCE=CTTGTAGAGTCAGCATGGAGGTT
PRIMER_RIGHT_14_SEQUENCE=CTCTTCAGATCCTGACACCCTTA
PRIMER_LEFT_14=78,23
PRIMER_RIGHT_14=547,23
PRIMER_LEFT_14_TM=59.824
PRIMER_RIGHT_14_TM=59.759
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.5000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=470
PRIMER_PAIR_PENALTY_15=0.4192
PRIMER_LEFT_15_PENALTY=0.178426
PRIMER_RIGHT_15_PENALTY=0.240778
PRIMER_LEFT_15_SEQUENCE=TTGTAGAGTCAGCATGGAGGTTT
PRIMER_RIGHT_15_SEQUENCE=CTCTTCAGATCCTGACACCCTTA
PRIMER_LEFT_15=79,23
PRIMER_RIGHT_15=547,23
PRIMER_LEFT_15_TM=60.178
PRIMER_RIGHT_15_TM=59.759
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.5000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=469
PRIMER_PAIR_PENALTY_16=0.4232
PRIMER_LEFT_16_PENALTY=0.012719
PRIMER_RIGHT_16_PENALTY=0.410484
PRIMER_LEFT_16_SEQUENCE=TGGAAAGGTGGTTCTTTAGATCA
PRIMER_RIGHT_16_SEQUENCE=TGTAGTAACCCATCCAGACTTCG
PRIMER_LEFT_16=942,23
PRIMER_RIGHT_16=1361,23
PRIMER_LEFT_16_TM=59.987
PRIMER_RIGHT_16_TM=60.410
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=8.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=420
PRIMER_PAIR_PENALTY_17=0.4600
PRIMER_LEFT_17_PENALTY=0.049500
PRIMER_RIGHT_17_PENALTY=0.410484
PRIMER_LEFT_17_SEQUENCE=TGGTTGTCTTCAACCACCTTACT
PRIMER_RIGHT_17_SEQUENCE=TGTAGTAACCCATCCAGACTTCG
PRIMER_LEFT_17=702,23
PRIMER_RIGHT_17=1361,23
PRIMER_LEFT_17_TM=59.950
PRIMER_RIGHT_17_TM=60.410
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=5.7000
PRIMER_RIGHT_17_END_STABILITY=8.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=660
PRIMER_PAIR_PENALTY_18=0.4600
PRIMER_LEFT_18_PENALTY=0.049500
PRIMER_RIGHT_18_PENALTY=0.410484
PRIMER_LEFT_18_SEQUENCE=CTGGTTGTCTTCAACCACCTTAC
PRIMER_RIGHT_18_SEQUENCE=TGTAGTAACCCATCCAGACTTCG
PRIMER_LEFT_18=701,23
PRIMER_RIGHT_18=1361,23
PRIMER_LEFT_18_TM=59.950
PRIMER_RIGHT_18_TM=60.410
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=8.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=8.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=661
PRIMER_PAIR_PENALTY_19=0.4690
PRIMER_LEFT_19_PENALTY=0.175797
PRIMER_RIGHT_19_PENALTY=0.293240
PRIMER_LEFT_19_SEQUENCE=CTTGTAGAGTCAGCATGGAGGTT
PRIMER_RIGHT_19_SEQUENCE=CACCTTTGTTGGTTTCACTCTCT
PRIMER_LEFT_19=78,23
PRIMER_RIGHT_19=1055,23
PRIMER_LEFT_19_TM=59.824
PRIMER_RIGHT_19_TM=59.707
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=978
=