PRIMER_SEQUENCE_ID=NM_001721
SEQUENCE=CGGACGCGTGGGCGGACGCGTGGGCGACCCACGCGTCCGGTGATGGTGCCTCAAAGCAGTAACTTTTTGCTTAGAGCTTGAGAGTCAAAGTTAAGGACCCACATGTATACTTCGGCTCTAGCGAGTCTAAGGATGATAATATGGATACAAAATCTATTCTAGAAGAACTTCTTCTCAAAAGATCACAGCAAAAGAAGAAAATGTCACCAAATAATTACAAAGAACGGCTTTTTGTTTTGACCAAAACAAACCTTTCCTACTATGAATATGACAAAATGAAAAGGGGCAGCAGAAAAGGATCCATTGAAATTAAGAAAATCAGATGTGTGGAGAAAGTAAATCTCGAGGAGCAGACGCCTGTAGAGAGACAGTACCCATTTCAGATTGTCTATAAAGATGGGCTTCTCTATGTCTATGCATCAAATGAAGAGAGCCGAAGTCAGTGGTTGAAAGCATTACAAAAAGAGATAAGGGGTAACCCCCACCTGCTGGTCAAGTACCATAGTGGGTTCTTCGTGGACGGGAAGTTCCTGTGTTGCCAGCAGAGCTGTAAAGCAGCCCCAGGATGTACCCTCTGGGAAGCATATGCTAATCTGCATACTGCAGTCAATGAAGAGAAACACAGAGTTCCCACCTTCCCAGACAGAGTGCTGAAGATACCTCGGGCAGTTCCTGTTCTCAAAATGGATGCACCATCTTCAAGTACCACTCTAGCCCAATATGACAACGAATCAAAGAAAAACTATGGCTCCCAGCCACCATCTTCAAGTACCAGTCTAGCGCAATATGACAGCAACTCAAAGAAAATCTATGGCTCCCAGCCAAACTTCAACATGCAGTATATTCCAAGGGAAGACTTCCCTGACTGGTGGCAAGTAAGAAAACTGAAAAGTAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAAATGTGAATCACACCACCTCAAAGATTTCATGGGAATTCCCTGAGTCAAGTTCATCTGAAGAAGAGGAAAACCTGGATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGTTACTCAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTAGAAATTCGAGCCAAGTGGGAATGTACACAGTGTCCTTATTTAGTAAGGCTGTGAATGATAAAAAAGGAACTGTCAAACATTACCACGTGCATACAAATGCTGAGAACAAATTATACCTGGCAGAAAACTACTGTTTTGATTCCATTCCAAAGCTTATTCATTATCATCAACACAATTCAGCAGGCATGATCACACGGCTCCGCCACCCTGTGTCAACAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAATCTGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCAGTTTGGAGTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCAAGGAGGGCTCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACTATGATGAAACTCAGCCATCCCAAGCTGGTTAAATTCTATGGAGTGTGTTCAAAGGAATACCCCATATACATAGTGACTGAATATATAAGCAATGGCTGCTTGCTGAATTACCTGAGGAGTCACGGAAAAGGACTTGAACCTTCCCAGCTCTTAGAAATGTGCTACGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGTCACCAATTCATACACCGGGACTTGGCTGCTCGTAACTGCTTGGTGGACAGAGATCTCTGTGTGAAAGTATCTGACTTTGGAATGACAAGGTATGTTCTTGATGACCAGTATGTCAGTTCAGTCGGAACAAAGTTTCCAGTCAAGTGGTCAGCTCCAGAGGTGTTTCATTACTTCAAATACAGCAGCAAGTCAGACGTATGGGCATTTGGGATCCTGATGTGGGAGGTGTTCAGCCTGGGGAAGCAGCCCTATGACTTGTATGACAACTCCCAGGTGGTTCTGAAGGTCTCCCAGGGCCACAGGCTTTACCGGCCCCACCTGGCATCGGACACCATCTACCAGATCATGTACAGCTGCTGGCACGAGCTTCCAGAAAAGCGTCCCACATTTCAGCAACTCCTGTCTTCCATTGAACCACTTCGGGAAAAAGACAAGCATTGAAGAAGAAATTAGGAGTGCTGATAAGAATGAATATAGATGCTGGCCAGCATTTTCATTCATTTTAAGGAAAGTAGCAAGGCATAATGTAATTTAGCTAGTTTTTAATAGTGTTCTCTGTATTGTCTATTATTTAGAAATGAACAAGGCAGGAAACAAAAGATTCCCTTGAAATTTAGATCAAATTAGTAATTTTGTTTATGCTGCTCCTGATATAACACTTTCCAGCCTATAGCAGAAGCACATTTTCAGACTGCAATATAGAGACTGTGTTCATGTGTAAAGACTGAGCAGAACTGAAAAATTACTTATTGGATATTCATTCTTTTCTTTATATTGTCATTGTCACAACAATTAAATATACTACCAAGTAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 29283, GC content failed 598, low tm 15417, high tm 6422, high any compl 22, high end compl 66, high repeat similarity 42, long poly-x seq 21, ok 6695
PRIMER_RIGHT_EXPLAIN=considered 29210, GC content failed 690, low tm 15680, high tm 6128, high any compl 5, high end compl 10, high repeat similarity 13, long poly-x seq 30, ok 6654
PRIMER_PAIR_EXPLAIN=considered 665, unacceptable product size 590, high any compl 4, high end compl 7, ok 64
PRIMER_PAIR_PENALTY=0.1581
PRIMER_LEFT_PENALTY=0.100269
PRIMER_RIGHT_PENALTY=0.057845
PRIMER_LEFT_SEQUENCE=TTGACCAAAACAAACCTTTCCTA
PRIMER_RIGHT_SEQUENCE=AGTGGTACTTGAAGATGGTGCAT
PRIMER_LEFT=236,23
PRIMER_RIGHT=709,23
PRIMER_LEFT_TM=59.900
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=474
PRIMER_PAIR_PENALTY_1=0.1694
PRIMER_LEFT_1_PENALTY=0.100269
PRIMER_RIGHT_1_PENALTY=0.069179
PRIMER_LEFT_1_SEQUENCE=TTGACCAAAACAAACCTTTCCTA
PRIMER_RIGHT_1_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_1=236,23
PRIMER_RIGHT_1=975,23
PRIMER_LEFT_1_TM=59.900
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=740
PRIMER_PAIR_PENALTY_2=0.1759
PRIMER_LEFT_2_PENALTY=0.061028
PRIMER_RIGHT_2_PENALTY=0.114860
PRIMER_LEFT_2_SEQUENCE=GGAACAAAGTTTCCAGTCAAGTG
PRIMER_RIGHT_2_SEQUENCE=TTTCCTGCCTTGTTCATTTCTAA
PRIMER_LEFT_2=1850,23
PRIMER_RIGHT_2=2320,23
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=471
PRIMER_PAIR_PENALTY_3=0.1763
PRIMER_LEFT_3_PENALTY=0.115306
PRIMER_RIGHT_3_PENALTY=0.061028
PRIMER_LEFT_3_SEQUENCE=CTGAAGAAGAGGAAAACCTGGAT
PRIMER_RIGHT_3_SEQUENCE=CCACTTGACTGGAAACTTTGTTC
PRIMER_LEFT_3=993,23
PRIMER_RIGHT_3=1873,23
PRIMER_LEFT_3_TM=60.115
PRIMER_RIGHT_3_TM=60.061
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=881
PRIMER_PAIR_PENALTY_4=0.1922
PRIMER_LEFT_4_PENALTY=0.122991
PRIMER_RIGHT_4_PENALTY=0.069179
PRIMER_LEFT_4_SEQUENCE=CTAGCCCAATATGACAACGAATC
PRIMER_RIGHT_4_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_4=710,23
PRIMER_RIGHT_4=975,23
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=60.069
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=266
PRIMER_PAIR_PENALTY_5=0.1922
PRIMER_LEFT_5_PENALTY=0.122991
PRIMER_RIGHT_5_PENALTY=0.069179
PRIMER_LEFT_5_SEQUENCE=TCTAGCCCAATATGACAACGAAT
PRIMER_RIGHT_5_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_5=709,23
PRIMER_RIGHT_5=975,23
PRIMER_LEFT_5_TM=59.877
PRIMER_RIGHT_5_TM=60.069
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.6000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=267
PRIMER_PAIR_PENALTY_6=0.2025
PRIMER_LEFT_6_PENALTY=0.133335
PRIMER_RIGHT_6_PENALTY=0.069179
PRIMER_LEFT_6_SEQUENCE=ATCAAAGAAAAACTATGGCTCCC
PRIMER_RIGHT_6_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_6=730,23
PRIMER_RIGHT_6=975,23
PRIMER_LEFT_6_TM=59.867
PRIMER_RIGHT_6_TM=60.069
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MER11A a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=7.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=246
PRIMER_PAIR_PENALTY_7=0.2053
PRIMER_LEFT_7_PENALTY=0.144318
PRIMER_RIGHT_7_PENALTY=0.061028
PRIMER_LEFT_7_SEQUENCE=CCAGACTATGATGAAACTCAGCC
PRIMER_RIGHT_7_SEQUENCE=CCACTTGACTGGAAACTTTGTTC
PRIMER_LEFT_7=1522,23
PRIMER_RIGHT_7=1873,23
PRIMER_LEFT_7_TM=60.144
PRIMER_RIGHT_7_TM=60.061
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=352
PRIMER_PAIR_PENALTY_8=0.2086
PRIMER_LEFT_8_PENALTY=0.150712
PRIMER_RIGHT_8_PENALTY=0.057845
PRIMER_LEFT_8_SEQUENCE=TGAGAGTCAAAGTTAAGGACCCA
PRIMER_RIGHT_8_SEQUENCE=AGTGGTACTTGAAGATGGTGCAT
PRIMER_LEFT_8=78,23
PRIMER_RIGHT_8=709,23
PRIMER_LEFT_8_TM=60.151
PRIMER_RIGHT_8_TM=59.942
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=632
PRIMER_PAIR_PENALTY_9=0.2109
PRIMER_LEFT_9_PENALTY=0.115306
PRIMER_RIGHT_9_PENALTY=0.095601
PRIMER_LEFT_9_SEQUENCE=CTGAAGAAGAGGAAAACCTGGAT
PRIMER_RIGHT_9_SEQUENCE=GAATTCATCTTCTGACATGGAGC
PRIMER_LEFT_9=993,23
PRIMER_RIGHT_9=1510,23
PRIMER_LEFT_9_TM=60.115
PRIMER_RIGHT_9_TM=60.096
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=8.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=518
PRIMER_PAIR_PENALTY_10=0.2156
PRIMER_LEFT_10_PENALTY=0.100269
PRIMER_RIGHT_10_PENALTY=0.115306
PRIMER_LEFT_10_SEQUENCE=TTGACCAAAACAAACCTTTCCTA
PRIMER_RIGHT_10_SEQUENCE=CCAGGTTTTCCTCTTCTTCAGAT
PRIMER_LEFT_10=236,23
PRIMER_RIGHT_10=1013,23
PRIMER_LEFT_10_TM=59.900
PRIMER_RIGHT_10_TM=60.115
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=7.2000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=778
PRIMER_PAIR_PENALTY_11=0.2186
PRIMER_LEFT_11_PENALTY=0.122991
PRIMER_RIGHT_11_PENALTY=0.095601
PRIMER_LEFT_11_SEQUENCE=CTAGCCCAATATGACAACGAATC
PRIMER_RIGHT_11_SEQUENCE=GAATTCATCTTCTGACATGGAGC
PRIMER_LEFT_11=710,23
PRIMER_RIGHT_11=1510,23
PRIMER_LEFT_11_TM=59.877
PRIMER_RIGHT_11_TM=60.096
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.6000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=801
PRIMER_PAIR_PENALTY_12=0.2186
PRIMER_LEFT_12_PENALTY=0.122991
PRIMER_RIGHT_12_PENALTY=0.095601
PRIMER_LEFT_12_SEQUENCE=TCTAGCCCAATATGACAACGAAT
PRIMER_RIGHT_12_SEQUENCE=GAATTCATCTTCTGACATGGAGC
PRIMER_LEFT_12=709,23
PRIMER_RIGHT_12=1510,23
PRIMER_LEFT_12_TM=59.877
PRIMER_RIGHT_12_TM=60.096
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.6000
PRIMER_RIGHT_12_END_STABILITY=9.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=802
PRIMER_PAIR_PENALTY_13=0.2199
PRIMER_LEFT_13_PENALTY=0.150712
PRIMER_RIGHT_13_PENALTY=0.069179
PRIMER_LEFT_13_SEQUENCE=TGAGAGTCAAAGTTAAGGACCCA
PRIMER_RIGHT_13_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_13=78,23
PRIMER_RIGHT_13=975,23
PRIMER_LEFT_13_TM=60.151
PRIMER_RIGHT_13_TM=60.069
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=898
PRIMER_PAIR_PENALTY_14=0.2206
PRIMER_LEFT_14_PENALTY=0.100269
PRIMER_RIGHT_14_PENALTY=0.120296
PRIMER_LEFT_14_SEQUENCE=TTGACCAAAACAAACCTTTCCTA
PRIMER_RIGHT_14_SEQUENCE=GCTCCTCGAGATTTACTTTCTCC
PRIMER_LEFT_14=236,23
PRIMER_RIGHT_14=350,23
PRIMER_LEFT_14_TM=59.900
PRIMER_RIGHT_14_TM=59.880
PRIMER_LEFT_14_GC_PERCENT=34.783
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER4A a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MER4A a consensus
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=115
PRIMER_PAIR_PENALTY_15=0.2233
PRIMER_LEFT_15_PENALTY=0.100269
PRIMER_RIGHT_15_PENALTY=0.122991
PRIMER_LEFT_15_SEQUENCE=TTGACCAAAACAAACCTTTCCTA
PRIMER_RIGHT_15_SEQUENCE=ATTCGTTGTCATATTGGGCTAGA
PRIMER_LEFT_15=236,23
PRIMER_RIGHT_15=731,23
PRIMER_LEFT_15_TM=59.900
PRIMER_RIGHT_15_TM=59.877
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.2000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=496
PRIMER_PAIR_PENALTY_16=0.2237
PRIMER_LEFT_16_PENALTY=0.061028
PRIMER_RIGHT_16_PENALTY=0.162674
PRIMER_LEFT_16_SEQUENCE=GGAACAAAGTTTCCAGTCAAGTG
PRIMER_RIGHT_16_SEQUENCE=CATACAAGTCATAGGGCTGCTTC
PRIMER_LEFT_16=1850,23
PRIMER_RIGHT_16=1988,23
PRIMER_LEFT_16_TM=60.061
PRIMER_RIGHT_16_TM=60.163
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=139
PRIMER_PAIR_PENALTY_17=0.2237
PRIMER_LEFT_17_PENALTY=0.061028
PRIMER_RIGHT_17_PENALTY=0.162674
PRIMER_LEFT_17_SEQUENCE=GGAACAAAGTTTCCAGTCAAGTG
PRIMER_RIGHT_17_SEQUENCE=TCATACAAGTCATAGGGCTGCTT
PRIMER_LEFT_17=1850,23
PRIMER_RIGHT_17=1989,23
PRIMER_LEFT_17_TM=60.061
PRIMER_RIGHT_17_TM=60.163
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=140
PRIMER_PAIR_PENALTY_18=0.2244
PRIMER_LEFT_18_PENALTY=0.128838
PRIMER_RIGHT_18_PENALTY=0.095601
PRIMER_LEFT_18_SEQUENCE=CATGCAGTATATTCCAAGGGAAG
PRIMER_RIGHT_18_SEQUENCE=GAATTCATCTTCTGACATGGAGC
PRIMER_LEFT_18=832,23
PRIMER_RIGHT_18=1510,23
PRIMER_LEFT_18_TM=59.871
PRIMER_RIGHT_18_TM=60.096
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=9.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=679
PRIMER_PAIR_PENALTY_19=0.2270
PRIMER_LEFT_19_PENALTY=0.157853
PRIMER_RIGHT_19_PENALTY=0.069179
PRIMER_LEFT_19_SEQUENCE=TGGTCAAGTACCATAGTGGGTTC
PRIMER_RIGHT_19_SEQUENCE=AATTCCCATGAAATCTTTGAGGT
PRIMER_LEFT_19=489,23
PRIMER_RIGHT_19=975,23
PRIMER_LEFT_19_TM=60.158
PRIMER_RIGHT_19_TM=60.069
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=487
=