PRIMER_SEQUENCE_ID=NM_001982
SEQUENCE=CTCTCACACACACACACCCCTCCCCTGCCATCCCTCCCCGGACTCCGGCTCCGGCTCCGATTGCAATTTGCAACCTCCGCTGCCGTCGCCGCAGCAGCCACCAATTCGCCAGCGGTTCAGGTGGCTCTTGCCTCGATGTCCTAGCCTAGGGGCCCCCGGGCCGGACTTGGCTGGGCTCCCTTCACCCTCTGCGGAGTCATGAGGGCGAACGACGCTCTGCAGGTGCTGGGCTTGCTTTTCAGCCTGGCCCGGGGCTCCGAGGTGGGCAACTCTCAGGCAGTGTGTCCTGGGACTCTGAATGGCCTGAGTGTGACCGGCGATGCTGAGAACCAATACCAGACACTGTACAAGCTCTACGAGAGGTGTGAGGTGGTGATGGGGAACCTTGAGATTGTGCTCACGGGACACAATGCCGACCTCTCCTTCCTGCAGTGGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCTACTCTACCATTGCCCAACCTCCGCGTGGTGCGAGGGACCCAGGTCTACGATGGGAAGTTTGCCATCTTCGTCATGTTGAACTATAACACCAACTCCAGCCACGCTCTGCGCCAGCTCCGCTTGACTCAGCTCACCGAGATTCTGTCAGGGGGTGTTTATATTGAGAAGAACGATAAGCTTTGTCACATGGACACAATTGACTGGAGGGACATCGTGAGGGACCGAGATGCTGAGATAGTGGTGAAGGACAATGGCAGAAGCTGTCCCCCCTGTCATGAGGTTTGCAAGGGGCGATGCTGGGGTCCTGGATCAGAAGACTGCCAGACATTGACCAAGACCATCTGTGCTCCTCAGTGTAATGGTCACTGCTTTGGGCCCAACCCCAACCAGTGCTGCCATGATGAGTGTGCCGGGGGCTGCTCAGGCCCTCAGGACACAGACTGCTTTGCCTGCCGGCACTTCAATGACAGTGGAGCCTGTGTACCTCGCTGTCCACAGCCTCTTGTCTACAACAAGCTAACTTTCCAGCTGGAACCCAATCCCCACACCAAGTATCAGTATGGAGGAGTTTGTGTAGCCAGCTGTCCCCATAACTTTGTGGTGGATCAAACATCCTGTGTCAGGGCCTGTCCTCCTGACAAGATGGAAGTAGATAAAAATGGGCTCAAGATGTGTGAGCCTTGTGGGGGACTATGTCCCAAAGCCTGTGAGGGAACAGGCTCTGGGAGCCGCTTCCAGACTGTGGACTCGAGCAACATTGATGGATTTGTGAACTGCACCAAGATCCTGGGCAACCTGGACTTTCTGATCACCGGCCTCAATGGAGACCCCTGGCACAAGATCCCTGCCCTGGACCCAGAGAAGCTCAATGTCTTCCGGACAGTACGGGAGATCACAGGTTACCTGAACATCCAGTCCTGGCCGCCCCACATGCACAACTTCAGTGTTTTTTCCAATTTGACAACCATTGGAGGCAGAAGCCTCTACAACCGGGGCTTCTCATTGTTGATCATGAAGAACTTGAATGTCACATCTCTGGGCTTCCGATCCCTGAAGGAAATTAGTGCTGGGCGTATCTATATAAGTGCCAATAGGCAGCTCTGCTACCACCACTCTTTGAACTGGACCAAGGTGCTTCGGGGGCCTACGGAAGAGCGACTAGACATCAAGCATAATCGGCCGCGCAGAGACTGCGTGGCAGAGGGCAAAGTGTGTGACCCACTGTGCTCCTCTGGGGGATGCTGGGGCCCAGGCCCTGGTCAGTGCTTGTCCTGTCGAAATTATAGCCGAGGAGGTGTCTGTGTGACCCACTGCAACTTTCTGAATGGGGAGCCTCGAGAATTTGCCCATGAGGCCGAATGCTTCTCCTGCCACCCGGAATGCCAACCCATGGGGGGCACTGCCACATGCAATGGCTCGGGCTCTGATACTTGTGCTCAATGTGCCCATTTTCGAGATGGGCCCCACTGTGTGAGCAGCTGCCCCCATGGAGTCCTAGGTGCCAAGGGCCCAATCTACAAGTACCCAGATGTTCAGAATGAATGTCGGCCCTGCCATGAGAACTGCACCCAGGGGTGTAAAGGACCAGAGCTTCAAGACTGTTTAGGACAAACACTGGTGCTGATCGGCAAAACCCATCTGACAATGGCTTTGACAGTGATAGCAGGATTGGTAGTGATTTTCATGATGCTGGGCGGCACTTTTCTCTACTGGCGTGGGCGCCGGATTCAGAATAAAAGGGCTATGAGGCGATACTTGGAACGGGGTGAGAGCATAGAGCCTCTGGACCCCAGTGAGAAGGCTAACAAAGTCTTGGCCAGAATCTTCAAAGAGACAGAGCTAAGGAAGCTTAAAGTGCTTGGCTCGGGTGTCTTTGGAACTGTGCACAAAGGAGTGTGGATCCCTGAGGGTGAATCAATCAAGATTCCAGTCTGCATTAAAGTCATTGAGGACAAGAGTGGACGGCAGAGTTTTCAAGCTGTGACAGATCATATGCTGGCCATTGGCAGCCTGGACCATGCCCACATTGTAAGGCTGCTGGGACTATGCCCAGGGTCATCTCTGCAGCTTGTCACTCAATATTTGCCTCTGGGTTCTCTGCTGGATCATGTGAGACAACACCGGGGGGCACTGGGGCCACAGCTGCTGCTCAACTGGGGAGTACAAATTGCCAAGGGAATGTACTACCTTGAGGAACATGGTATGGTGCATAGAAACCTGGCTGCCCGAAACGTGCTACTCAAGTCACCCAGTCAGGTTCAGGTGGCAGATTTTGGTGTGGCTGACCTGCTGCCTCCTGATGATAAGCAGCTGCTATACAGTGAGGCCAAGACTCCAATTAAGTGGATGGCCCTTGAGAGTATCCACTTTGGGAAATACACACACCAGAGTGATGTCTGGAGCTATGGTGTGACAGTTTGGGAGTTGATGACCTTCGGGGCAGAGCCCTATGCAGGGCTACGATTGGCTGAAGTACCAGACCTGCTAGAGAAGGGGGAGCGGTTGGCACAGCCCCAGATCTGCACAATTGATGTCTACATGGTGATGGTCAAGTGTTGGATGATTGATGAGAACATTCGCCCAACCTTTAAAGAACTAGCCAATGAGTTCACCAGGATGGCCCGAGACCCACCACGGTATCTGGTCATAAAGAGAGAGAGTGGGCCTGGAATAGCCCCTGGGCCAGAGCCCCATGGTCTGACAAACAAGAAGCTAGAGGAAGTAGAGCTGGAGCCAGAACTAGACCTAGACCTAGACTTGGAAGCAGAGGAGGACAACCTGGCAACCACCACACTGGGCTCCGCCCTCAGCCTACCAGTTGGAACACTTAATCGGCCACGTGGGAGCCAGAGCCTTTTAAGTCCATCATCTGGATACATGCCCATGAACCAGGGTAATCTTGGGGGGTCTTGCCAGGAGTCTGCAGTTTCTGGGAGCAGTGAACGGTGCCCCCGTCCAGTCTCTCTACACCCAATGCCACGGGGATGCCTGGCATCAGAGTCATCAGAGGGGCATGTAACAGGCTCTGAGGCTGAGCTCCAGGAGAAAGTGTCAATGTGTAGAAGCCGGAGCAGGAGCCGGAGCCCACGGCCACGCGGAGATAGCGCCTACCATTCCCAGCGCCACAGTCTGCTGACTCCTGTTACCCCACTCTCCCCACCCGGGTTAGAGGAAGAGGATGTCAACGGTTATGTCATGCCAGATACACACCTCAAAGGTACTCCCTCCTCCCGGGAAGGCACCCTTTCTTCAGTGGGTCTCAGTTCTGTCCTGGGTACTGAAGAAGAAGATGAAGATGAGGAGTATGAATACATGAACCGGAGGAGAAGGCACAGTCCACCTCATCCCCCTAGGCCAAGTTCCCTTGAGGAGCTGGGTTATGAGTACATGGATGTGGGGTCAGACCTCAGTGCCTCTCTGGGCAGCACACAGAGTTGCCCACTCCACCCTGTACCCATCATGCCCACTGCAGGCACAACTCCAGATGAAGACTATGAATATATGAATCGGCAACGAGATGGAGGTGGTCCTGGGGGTGATTATGCAGCCATGGGGGCCTGCCCAGCATCTGAGCAAGGGTATGAAGAGATGAGAGCTTTTCAGGGGCCTGGACATCAGGCCCCCCATGTCCATTATGCCCGCCTAAAAACTCTACGTAGCTTAGAGGCTACAGACTCTGCCTTTGATAACCCTGATTACTGGCATAGCAGGCTTTTCCCCAAGGCTAATGCCCAGAGAACGTAACTCCTGCTCCCTGTGGCACTCAGGGAGCATTTAATGGCAGCTAGTGCCTTTAGAGGGTACCGTCTTCTCCCTATTCCCTCTCTCTCCCAGGTCCCAGCCCCTTTTCCCCAGTCCCAGACAATTCCATTCAATCTTTGGAGGCTTTTAAACATTTTGACACAAAATTCTTATGGTATGTAGCCAGCTGTGCACTTTCTTCTCTTTCCCAACCCCAGGAAAGGTTTTCCTTATTTTGTGTGCTTTCCCAGTCCCATTCCTCAGCTTCTTCACAGGCACTCCTGGAGATATGAAGGATTACTCTCCATATCCCTTCCTCTCAGGCTCTTGACTACTTGGAACTAGGCTCTTATGTGTGCCTTTGTTTCCCATCAGACTGTCAAGAAGAGGAAAGGGAGGAAACCTAGCAGAGGAAAGTGTAATTTTGGTTTATGACTCTTAACCCCCTAGAAAGACAGAAGCTTAAAATCTGTGAAGAAAGAGGTTAGGAGTAGATATTGATTACTATCATAATTCAGCACTTAACTATGAGCCAGGCATCATACTAAACTTCACCTACATTATCTCACTTAGTCCTTTATCATCCTTAAAACAATTCTGTGACATACATATTATCTCATTTTACACAAAGGGAAGTCGGGCATGGTGGCTCATGCCTGTAATCTCAGCACTTTGGGAGGCTGAGGCAGAAGGATTACCTGAGGCAAGGAGTTTGAGACCAGCTTAGCCAACATAGTAAGACCCCCATCTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 57551, GC content failed 645, low tm 21180, high tm 23196, high any compl 1, high end compl 17, high repeat similarity 23, long poly-x seq 126, ok 12363
PRIMER_RIGHT_EXPLAIN=considered 57540, GC content failed 548, low tm 21528, high tm 22808, high any compl 3, high end compl 8, high repeat similarity 12, long poly-x seq 126, ok 12507
PRIMER_PAIR_EXPLAIN=considered 746, unacceptable product size 703, high end compl 2, ok 41
PRIMER_PAIR_PENALTY=0.0250
PRIMER_LEFT_PENALTY=0.020535
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=TGGCCATGAATGAATTCTCTACT
PRIMER_RIGHT_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT=463,23
PRIMER_RIGHT=1013,23
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=551
PRIMER_PAIR_PENALTY_1=0.0269
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.012719
PRIMER_LEFT_1_SEQUENCE=AGCAGGATTGGTAGTGATTTTCA
PRIMER_RIGHT_1_SEQUENCE=TTTCCCAAAGTGGATACTCTCAA
PRIMER_LEFT_1=2144,23
PRIMER_RIGHT_1=2858,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=715
PRIMER_PAIR_PENALTY_2=0.0298
PRIMER_LEFT_2_PENALTY=0.015613
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=GATCCCTGAAGGAAATTAGTGCT
PRIMER_RIGHT_2_SEQUENCE=TGAAAATCACTACCAATCCTGCT
PRIMER_LEFT_2=1528,23
PRIMER_RIGHT_2=2166,23
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=639
PRIMER_PAIR_PENALTY_3=0.0636
PRIMER_LEFT_3_PENALTY=0.059210
PRIMER_RIGHT_3_PENALTY=0.004430
PRIMER_LEFT_3_SEQUENCE=AACTATAACACCAACTCCAGCCA
PRIMER_RIGHT_3_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_3=561,23
PRIMER_RIGHT_3=1013,23
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=453
PRIMER_PAIR_PENALTY_4=0.0785
PRIMER_LEFT_4_PENALTY=0.064245
PRIMER_RIGHT_4_PENALTY=0.014220
PRIMER_LEFT_4_SEQUENCE=ACAGTACGGGAGATCACAGGTTA
PRIMER_RIGHT_4_SEQUENCE=TGAAAATCACTACCAATCCTGCT
PRIMER_LEFT_4=1362,23
PRIMER_RIGHT_4=2166,23
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=805
PRIMER_PAIR_PENALTY_5=0.0848
PRIMER_LEFT_5_PENALTY=0.020535
PRIMER_RIGHT_5_PENALTY=0.064245
PRIMER_LEFT_5_SEQUENCE=TGGCCATGAATGAATTCTCTACT
PRIMER_RIGHT_5_SEQUENCE=TAACCTGTGATCTCCCGTACTGT
PRIMER_LEFT_5=463,23
PRIMER_RIGHT_5=1384,23
PRIMER_LEFT_5_TM=59.979
PRIMER_RIGHT_5_TM=59.936
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.4000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=922
PRIMER_PAIR_PENALTY_6=0.0895
PRIMER_LEFT_6_PENALTY=0.085102
PRIMER_RIGHT_6_PENALTY=0.004430
PRIMER_LEFT_6_SEQUENCE=AGACATTGACCAAGACCATCTGT
PRIMER_RIGHT_6_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_6=805,23
PRIMER_RIGHT_6=1013,23
PRIMER_LEFT_6_TM=59.915
PRIMER_RIGHT_6_TM=59.996
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=209
PRIMER_PAIR_PENALTY_7=0.0941
PRIMER_LEFT_7_PENALTY=0.081398
PRIMER_RIGHT_7_PENALTY=0.012719
PRIMER_LEFT_7_SEQUENCE=TACTCAAGTCACCCAGTCAGGTT
PRIMER_RIGHT_7_SEQUENCE=TTTCCCAAAGTGGATACTCTCAA
PRIMER_LEFT_7=2719,23
PRIMER_RIGHT_7=2858,23
PRIMER_LEFT_7_TM=60.081
PRIMER_RIGHT_7_TM=59.987
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=140
PRIMER_PAIR_PENALTY_8=0.0956
PRIMER_LEFT_8_PENALTY=0.014220
PRIMER_RIGHT_8_PENALTY=0.081398
PRIMER_LEFT_8_SEQUENCE=AGCAGGATTGGTAGTGATTTTCA
PRIMER_RIGHT_8_SEQUENCE=AACCTGACTGGGTGACTTGAGTA
PRIMER_LEFT_8=2144,23
PRIMER_RIGHT_8=2741,23
PRIMER_LEFT_8_TM=60.014
PRIMER_RIGHT_8_TM=60.081
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER8 Nonautonomous DNA transposon
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=5.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=598
PRIMER_PAIR_PENALTY_9=0.1028
PRIMER_LEFT_9_PENALTY=0.014220
PRIMER_RIGHT_9_PENALTY=0.088604
PRIMER_LEFT_9_SEQUENCE=AGCAGGATTGGTAGTGATTTTCA
PRIMER_RIGHT_9_SEQUENCE=TCTAGCAGGTCTGGTACTTCAGC
PRIMER_LEFT_9=2144,23
PRIMER_RIGHT_9=2971,23
PRIMER_LEFT_9_TM=60.014
PRIMER_RIGHT_9_TM=60.089
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=52.174
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=828
PRIMER_PAIR_PENALTY_10=0.1056
PRIMER_LEFT_10_PENALTY=0.020535
PRIMER_RIGHT_10_PENALTY=0.085102
PRIMER_LEFT_10_SEQUENCE=TGGCCATGAATGAATTCTCTACT
PRIMER_RIGHT_10_SEQUENCE=ACAGATGGTCTTGGTCAATGTCT
PRIMER_LEFT_10=463,23
PRIMER_RIGHT_10=827,23
PRIMER_LEFT_10_TM=59.979
PRIMER_RIGHT_10_TM=59.915
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=365
PRIMER_PAIR_PENALTY_11=0.1200
PRIMER_LEFT_11_PENALTY=0.115612
PRIMER_RIGHT_11_PENALTY=0.004430
PRIMER_LEFT_11_SEQUENCE=TGTCAGGGGGTGTTTATATTGAG
PRIMER_RIGHT_11_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_11=625,23
PRIMER_RIGHT_11=1013,23
PRIMER_LEFT_11_TM=60.116
PRIMER_RIGHT_11_TM=59.996
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=389
PRIMER_PAIR_PENALTY_12=0.1200
PRIMER_LEFT_12_PENALTY=0.115612
PRIMER_RIGHT_12_PENALTY=0.004430
PRIMER_LEFT_12_SEQUENCE=TCTGTCAGGGGGTGTTTATATTG
PRIMER_RIGHT_12_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_12=623,23
PRIMER_RIGHT_12=1013,23
PRIMER_LEFT_12_TM=60.116
PRIMER_RIGHT_12_TM=59.996
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=391
PRIMER_PAIR_PENALTY_13=0.1200
PRIMER_LEFT_13_PENALTY=0.115612
PRIMER_RIGHT_13_PENALTY=0.004430
PRIMER_LEFT_13_SEQUENCE=CTGTCAGGGGGTGTTTATATTGA
PRIMER_RIGHT_13_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_13=624,23
PRIMER_RIGHT_13=1013,23
PRIMER_LEFT_13_TM=60.116
PRIMER_RIGHT_13_TM=59.996
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=390
PRIMER_PAIR_PENALTY_14=0.1235
PRIMER_LEFT_14_PENALTY=0.059210
PRIMER_RIGHT_14_PENALTY=0.064245
PRIMER_LEFT_14_SEQUENCE=AACTATAACACCAACTCCAGCCA
PRIMER_RIGHT_14_SEQUENCE=TAACCTGTGATCTCCCGTACTGT
PRIMER_LEFT_14=561,23
PRIMER_RIGHT_14=1384,23
PRIMER_LEFT_14_TM=59.941
PRIMER_RIGHT_14_TM=59.936
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=824
PRIMER_PAIR_PENALTY_15=0.1265
PRIMER_LEFT_15_PENALTY=0.122060
PRIMER_RIGHT_15_PENALTY=0.004430
PRIMER_LEFT_15_SEQUENCE=AGATTCTGTCAGGGGGTGTTTAT
PRIMER_RIGHT_15_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_15=619,23
PRIMER_RIGHT_15=1013,23
PRIMER_LEFT_15_TM=60.122
PRIMER_RIGHT_15_TM=59.996
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.2000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=395
PRIMER_PAIR_PENALTY_16=0.1266
PRIMER_LEFT_16_PENALTY=0.122120
PRIMER_RIGHT_16_PENALTY=0.004430
PRIMER_LEFT_16_SEQUENCE=GAGATAGTGGTGAAGGACAATGG
PRIMER_RIGHT_16_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_16=714,23
PRIMER_RIGHT_16=1013,23
PRIMER_LEFT_16_TM=59.878
PRIMER_RIGHT_16_TM=59.996
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=300
PRIMER_PAIR_PENALTY_17=0.1284
PRIMER_LEFT_17_PENALTY=0.123924
PRIMER_RIGHT_17_PENALTY=0.004430
PRIMER_LEFT_17_SEQUENCE=ACAAGCTCTACGAGAGGTGTGAG
PRIMER_RIGHT_17_SEQUENCE=CAGCTGGAAAGTTAGCTTGTTGT
PRIMER_LEFT_17=346,23
PRIMER_RIGHT_17=1013,23
PRIMER_LEFT_17_TM=60.124
PRIMER_RIGHT_17_TM=59.996
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=668
PRIMER_PAIR_PENALTY_18=0.1339
PRIMER_LEFT_18_PENALTY=0.121208
PRIMER_RIGHT_18_PENALTY=0.012719
PRIMER_LEFT_18_SEQUENCE=ATAAAAGGGCTATGAGGCGATAC
PRIMER_RIGHT_18_SEQUENCE=TTTCCCAAAGTGGATACTCTCAA
PRIMER_LEFT_18=2215,23
PRIMER_RIGHT_18=2858,23
PRIMER_LEFT_18_TM=59.879
PRIMER_RIGHT_18_TM=59.987
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.3000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=644
PRIMER_PAIR_PENALTY_19=0.1361
PRIMER_LEFT_19_PENALTY=0.020535
PRIMER_RIGHT_19_PENALTY=0.115612
PRIMER_LEFT_19_SEQUENCE=TGGCCATGAATGAATTCTCTACT
PRIMER_RIGHT_19_SEQUENCE=CTCAATATAAACACCCCCTGACA
PRIMER_LEFT_19=463,23
PRIMER_RIGHT_19=647,23
PRIMER_LEFT_19_TM=59.979
PRIMER_RIGHT_19_TM=60.116
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.4000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=185
=