PRIMER_SEQUENCE_ID=NM_002011
SEQUENCE=CCGAGGAGCGCTCGGGCTGTCTGCGGACCCTGCCGCGTGCAGGGGTCGCGGCCGGCTGGAGCTGGGAGTGAGGCGGCGGAGGAGCCAGGTGAGGAGGAGCCAGGAAGGCAGTTGGTGGGAAGTCCAGCTTGGGTCCCTGAGAGCTGTGAGAAGGAGATGCGGCTGCTGCTGGCCCTGTTGGGGGTCCTGCTGAGTGTGCCTGGGCCTCCAGTCTTGTCCCTGGAGGCCTCTGAGGAAGTGGAGCTTGAGCCCTGCCTGGCTCCCAGCCTGGAGCAGCAAGAGCAGGAGCTGACAGTAGCCCTTGGGCAGCCTGTGCGGCTGTGCTGTGGGCGGGCTGAGCGTGGTGGCCACTGGTACAAGGAGGGCAGTCGCCTGGCACCTGCTGGCCGTGTACGGGGCTGGAGGGGCCGCCTAGAGATTGCCAGCTTCCTACCTGAGGATGCTGGCCGCTACCTCTGCCTGGCACGAGGCTCCATGATCGTCCTGCAGAATCTCACCTTGATTACAGGTGACTCCTTGACCTCCAGCAACGATGATGAGGACCCCAAGTCCCATAGGGACCTCTCGAATAGGCACAGTTACCCCCAGCAAGCACCCTACTGGACACACCCCCAGCGCATGGAGAAGAAACTGCATGCAGTACCTGCGGGGAACACCGTCAAGTTCCGCTGTCCAGCTGCAGGCAACCCCACGCCCACCATCCGCTGGCTTAAGGATGGACAGGCCTTTCATGGGGAGAACCGCATTGGAGGCATTCGGCTGCGCCATCAGCACTGGAGTCTCGTGATGGAGAGCGTGGTGCCCTCGGACCGCGGCACATACACCTGCCTGGTAGAGAACGCTGTGGGCAGCATCCGCTATAACTACCTGCTAGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCCGGGCTCCCGGCCAACACCACAGCCGTGGTGGGCAGCGACGTGGAGCTGCTGTGCAAGGTGTACAGCGATGCCCAGCCCCACATCCAGTGGCTGAAGCACATCGTCATCAACGGCAGCAGCTTCGGAGCCGACGGTTTCCCCTATGTGCAAGTCCTAAAGACTGCAGACATCAATAGCTCAGAGGTGGAGGTCCTGTACCTGCGGAACGTGTCAGCCGAGGACGCAGGCGAGTACACCTGCCTCGCAGGCAATTCCATCGGCCTCTCCTACCAGTCTGCCTGGCTCACGGTGCTGCCAGAGGAGGACCCCACATGGACCGCAGCAGCGCCCGAGGCCAGGTATACGGACATCATCCTGTACGCGTCGGGCTCCCTGGCCTTGGCTGTGCTCCTGCTGCTGGCCGGGCTGTATCGAGGGCAGGCGCTCCACGGCCGGCACCCCCGCCCGCCCGCCACTGTGCAGAAGCTCTCCCGCTTCCCTCTGGCCCGACAGTTCTCCCTGGAGTCAGGCTCTTCCGGCAAGTCAAGCTCATCCCTGGTACGAGGCGTGCGTCTCTCCTCCAGCGGCCCCGCCTTGCTCGCCGGCCTCGTGAGTCTAGATCTACCTCTCGACCCACTATGGGAGTTCCCCCGGGACAGGCTGGTGCTTGGGAAGCCCCTAGGCGAGGGCTGCTTTGGCCAGGTAGTACGTGCAGAGGCCTTTGGCATGGACCCTGCCCGGCCTGACCAAGCCAGCACTGTGGCCGTCAAGATGCTCAAAGACAACGCCTCTGACAAGGACCTGGCCGACCTGGTCTCGGAGATGGAGGTGATGAAGCTGATCGGCCGACACAAGAACATCATCAACCTGCTTGGTGTCTGCACCCAGGAAGGGCCCCTGTACGTGATCGTGGAGTGCGCCGCCAAGGGAAACCTGCGGGAGTTCCTGCGGGCCCGGCGCCCCCCAGGCCCCGACCTCAGCCCCGACGGTCCTCGGAGCAGTGAGGGGCCGCTCTCCTTCCCAGTCCTGGTCTCCTGCGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCCCGGAAGTGTATCCACCGGGACCTGGCTGCCCGCAATGTGCTGGTGACTGAGGACAATGTGATGAAGATTGCTGACTTTGGGCTGGCCCGCGGCGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCCTGTGAAGTGGATGGCGCCCGAGGCCTTGTTTGACCGGGTGTACACACACCAGAGTGACGTGTGGTCTTTTGGGATCCTGCTATGGGAGATCTTCACCCTCGGGGGCTCCCCGTATCCTGGCATCCCGGTGGAGGAGCTGTTCTCGCTGCTGCGGGAGGGACATCGGATGGACCGACCCCCACACTGCCCCCCAGAGCTGTACGGGCTGATGCGTGAGTGCTGGCACGCAGCGCCCTCCCAGAGGCCTACCTTCAAGCAGCTGGTGGAGGCGCTGGACAAGGTCCTGCTGGCCGTCTCTGAGGAGTACCTCGACCTCCGCCTGACCTTCGGACCCTATTCCCCCTCTGGTGGGGACGCCAGCAGCACCTGCTCCTCCAGCGATTCTGTCTTCAGCCACGACCCCCTGCCATTGGGATCCAGCTCCTTCCCCTTCGGGTCTGGGGTGCAGACATGAGCAAGGCTCAAGGCTGTGCAGGCACATAGGCTGGTGGCCTTGGGCCTTGGGGCTCAGCCACAGCCTGACACAGTGCTCGACCTTGATAGCATGGGGCCCCTGGCCCAGAGTTGCTGTGCCGTGTCCAAGGGCCGTGCCCTTGCCCTTGGAGCTGCCGTGCCTGTGTCCTGATGGCCCAAATGTCAGGGTTCTGCTCGGCTTCTTGGACCATGGCGCTTAGTCCCCATCCCGGGTTTGGCTGAGCCTGGCTGGAGAGCTGCTATGCTAAACCTCCTGCCTCCCAATACCAGCAGGAGGTTCTGGGCCTCTGAACCCCCTTTCCCCACACCTCCCCCTGCTGCTGCTGCCCCAGCGTCTTGACGGGAGCATTGGCCCCTGAGCCCAGAGAAGCTGGAAGCCTGCCGAAAACAGGAGCAAATGGCGTTTTATAAATTATTTTTTTGAAATAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 34821, GC content failed 1405, low tm 6033, high tm 21511, high any compl 12, high end compl 39, high repeat similarity 31, long poly-x seq 22, ok 5768
PRIMER_RIGHT_EXPLAIN=considered 34821, GC content failed 1336, low tm 6166, high tm 21349, high any compl 3, high end compl 12, high repeat similarity 12, long poly-x seq 24, ok 5919
PRIMER_PAIR_EXPLAIN=considered 614, unacceptable product size 560, high any compl 3, ok 51
PRIMER_PAIR_PENALTY=0.1130
PRIMER_LEFT_PENALTY=0.040223
PRIMER_RIGHT_PENALTY=0.072785
PRIMER_LEFT_SEQUENCE=CCTTGATTACAGGTGACTCCTTG
PRIMER_RIGHT_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT=496,23
PRIMER_RIGHT=1099,23
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.927
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=604
PRIMER_PAIR_PENALTY_1=0.2199
PRIMER_LEFT_1_PENALTY=0.040223
PRIMER_RIGHT_1_PENALTY=0.179693
PRIMER_LEFT_1_SEQUENCE=CCTTGATTACAGGTGACTCCTTG
PRIMER_RIGHT_1_SEQUENCE=TAGCAGGTAGTTATAGCGGATGC
PRIMER_LEFT_1=496,23
PRIMER_RIGHT_1=872,23
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=377
PRIMER_PAIR_PENALTY_2=0.2760
PRIMER_LEFT_2_PENALTY=0.203207
PRIMER_RIGHT_2_PENALTY=0.072785
PRIMER_LEFT_2_SEQUENCE=GACCTCTCGAATAGGCACAGTTA
PRIMER_RIGHT_2_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT_2=558,23
PRIMER_RIGHT_2=1099,23
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.927
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=542
PRIMER_PAIR_PENALTY_3=0.3829
PRIMER_LEFT_3_PENALTY=0.203207
PRIMER_RIGHT_3_PENALTY=0.179693
PRIMER_LEFT_3_SEQUENCE=GACCTCTCGAATAGGCACAGTTA
PRIMER_RIGHT_3_SEQUENCE=TAGCAGGTAGTTATAGCGGATGC
PRIMER_LEFT_3=558,23
PRIMER_RIGHT_3=872,23
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=59.820
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=315
PRIMER_PAIR_PENALTY_4=0.4819
PRIMER_LEFT_4_PENALTY=0.409123
PRIMER_RIGHT_4_PENALTY=0.072785
PRIMER_LEFT_4_SEQUENCE=TGATTACAGGTGACTCCTTGACC
PRIMER_RIGHT_4_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT_4=499,23
PRIMER_RIGHT_4=1099,23
PRIMER_LEFT_4_TM=60.409
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=601
PRIMER_PAIR_PENALTY_5=0.5497
PRIMER_LEFT_5_PENALTY=0.018344
PRIMER_RIGHT_5_PENALTY=0.531395
PRIMER_LEFT_5_SEQUENCE=AGGACAATGTGATGAAGATTGCT
PRIMER_RIGHT_5_SEQUENCE=ATTTATAAAACGCCATTTGCTCC
PRIMER_LEFT_5=2020,23
PRIMER_RIGHT_5=2997,23
PRIMER_LEFT_5_TM=60.018
PRIMER_RIGHT_5_TM=60.531
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=978
PRIMER_PAIR_PENALTY_6=0.5590
PRIMER_LEFT_6_PENALTY=0.027592
PRIMER_RIGHT_6_PENALTY=0.531395
PRIMER_LEFT_6_SEQUENCE=GTGACTGAGGACAATGTGATGAA
PRIMER_RIGHT_6_SEQUENCE=ATTTATAAAACGCCATTTGCTCC
PRIMER_LEFT_6=2013,23
PRIMER_RIGHT_6=2997,23
PRIMER_LEFT_6_TM=60.028
PRIMER_RIGHT_6_TM=60.531
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=985
PRIMER_PAIR_PENALTY_7=0.5888
PRIMER_LEFT_7_PENALTY=0.409123
PRIMER_RIGHT_7_PENALTY=0.179693
PRIMER_LEFT_7_SEQUENCE=TGATTACAGGTGACTCCTTGACC
PRIMER_RIGHT_7_SEQUENCE=TAGCAGGTAGTTATAGCGGATGC
PRIMER_LEFT_7=499,23
PRIMER_RIGHT_7=872,23
PRIMER_LEFT_7_TM=60.409
PRIMER_RIGHT_7_TM=59.820
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=374
PRIMER_PAIR_PENALTY_8=0.6810
PRIMER_LEFT_8_PENALTY=0.040223
PRIMER_RIGHT_8_PENALTY=0.640800
PRIMER_LEFT_8_SEQUENCE=CCTTGATTACAGGTGACTCCTTG
PRIMER_RIGHT_8_SEQUENCE=AGCAGGTAGTTATAGCGGATGCT
PRIMER_LEFT_8=496,23
PRIMER_RIGHT_8=871,23
PRIMER_LEFT_8_TM=60.040
PRIMER_RIGHT_8_TM=60.641
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=376
PRIMER_PAIR_PENALTY_9=0.7136
PRIMER_LEFT_9_PENALTY=0.640800
PRIMER_RIGHT_9_PENALTY=0.072785
PRIMER_LEFT_9_SEQUENCE=AGCATCCGCTATAACTACCTGCT
PRIMER_RIGHT_9_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT_9=849,23
PRIMER_RIGHT_9=1099,23
PRIMER_LEFT_9_TM=60.641
PRIMER_RIGHT_9_TM=59.927
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=251
PRIMER_PAIR_PENALTY_10=0.8077
PRIMER_LEFT_10_PENALTY=0.734897
PRIMER_RIGHT_10_PENALTY=0.072785
PRIMER_LEFT_10_SEQUENCE=CCTAGAGATTGCCAGCTTCCTAC
PRIMER_RIGHT_10_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT_10=410,23
PRIMER_RIGHT_10=1099,23
PRIMER_LEFT_10_TM=60.735
PRIMER_RIGHT_10_TM=59.927
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=690
PRIMER_PAIR_PENALTY_11=0.8077
PRIMER_LEFT_11_PENALTY=0.734897
PRIMER_RIGHT_11_PENALTY=0.072785
PRIMER_LEFT_11_SEQUENCE=CTAGAGATTGCCAGCTTCCTACC
PRIMER_RIGHT_11_SEQUENCE=ACCTCTGAGCTATTGATGTCTGC
PRIMER_LEFT_11=411,23
PRIMER_RIGHT_11=1099,23
PRIMER_LEFT_11_TM=60.735
PRIMER_RIGHT_11_TM=59.927
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=689
PRIMER_PAIR_PENALTY_12=0.8314
PRIMER_LEFT_12_PENALTY=0.040223
PRIMER_RIGHT_12_PENALTY=0.791133
PRIMER_LEFT_12_SEQUENCE=CCTTGATTACAGGTGACTCCTTG
PRIMER_RIGHT_12_SEQUENCE=GTCTTTAGGACTTGCACATAGGG
PRIMER_LEFT_12=496,23
PRIMER_RIGHT_12=1075,23
PRIMER_LEFT_12_TM=60.040
PRIMER_RIGHT_12_TM=59.209
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.7000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=580
PRIMER_PAIR_PENALTY_13=0.8440
PRIMER_LEFT_13_PENALTY=0.203207
PRIMER_RIGHT_13_PENALTY=0.640800
PRIMER_LEFT_13_SEQUENCE=GACCTCTCGAATAGGCACAGTTA
PRIMER_RIGHT_13_SEQUENCE=AGCAGGTAGTTATAGCGGATGCT
PRIMER_LEFT_13=558,23
PRIMER_RIGHT_13=871,23
PRIMER_LEFT_13_TM=59.797
PRIMER_RIGHT_13_TM=60.641
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=314
PRIMER_PAIR_PENALTY_14=0.8447
PRIMER_LEFT_14_PENALTY=0.313269
PRIMER_RIGHT_14_PENALTY=0.531395
PRIMER_LEFT_14_SEQUENCE=GTCTCTGAGGAGTACCTCGACCT
PRIMER_RIGHT_14_SEQUENCE=ATTTATAAAACGCCATTTGCTCC
PRIMER_LEFT_14=2403,23
PRIMER_RIGHT_14=2997,23
PRIMER_LEFT_14_TM=60.313
PRIMER_RIGHT_14_TM=60.531
PRIMER_LEFT_14_GC_PERCENT=56.522
PRIMER_RIGHT_14_GC_PERCENT=34.783
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=9.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=595
PRIMER_PAIR_PENALTY_15=0.8480
PRIMER_LEFT_15_PENALTY=0.316570
PRIMER_RIGHT_15_PENALTY=0.531395
PRIMER_LEFT_15_SEQUENCE=GCGTCCACCACATTGACTACTAT
PRIMER_RIGHT_15_SEQUENCE=ATTTATAAAACGCCATTTGCTCC
PRIMER_LEFT_15=2062,23
PRIMER_RIGHT_15=2997,23
PRIMER_LEFT_15_TM=60.317
PRIMER_RIGHT_15_TM=60.531
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=5.3000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=936
PRIMER_PAIR_PENALTY_16=0.8516
PRIMER_LEFT_16_PENALTY=0.837002
PRIMER_RIGHT_16_PENALTY=0.014618
PRIMER_LEFT_16_SEQUENCE=ACTGCAGACATCAATAGCTCAGA
PRIMER_RIGHT_16_SEQUENCE=CAATCTTCATCACATTGTCCTCA
PRIMER_LEFT_16=1074,23
PRIMER_RIGHT_16=2040,23
PRIMER_LEFT_16_TM=59.163
PRIMER_RIGHT_16_TM=59.985
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=967
PRIMER_PAIR_PENALTY_17=0.8553
PRIMER_LEFT_17_PENALTY=0.837002
PRIMER_RIGHT_17_PENALTY=0.018344
PRIMER_LEFT_17_SEQUENCE=ACTGCAGACATCAATAGCTCAGA
PRIMER_RIGHT_17_SEQUENCE=AGCAATCTTCATCACATTGTCCT
PRIMER_LEFT_17=1074,23
PRIMER_RIGHT_17=2042,23
PRIMER_LEFT_17_TM=59.163
PRIMER_RIGHT_17_TM=60.018
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=969
PRIMER_PAIR_PENALTY_18=0.8773
PRIMER_LEFT_18_PENALTY=0.862716
PRIMER_RIGHT_18_PENALTY=0.014618
PRIMER_LEFT_18_SEQUENCE=GATCTACCTCTCGACCCACTATG
PRIMER_RIGHT_18_SEQUENCE=CAATCTTCATCACATTGTCCTCA
PRIMER_LEFT_18=1512,23
PRIMER_RIGHT_18=2040,23
PRIMER_LEFT_18_TM=59.137
PRIMER_RIGHT_18_TM=59.985
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=529
PRIMER_PAIR_PENALTY_19=0.8811
PRIMER_LEFT_19_PENALTY=0.862716
PRIMER_RIGHT_19_PENALTY=0.018344
PRIMER_LEFT_19_SEQUENCE=GATCTACCTCTCGACCCACTATG
PRIMER_RIGHT_19_SEQUENCE=AGCAATCTTCATCACATTGTCCT
PRIMER_LEFT_19=1512,23
PRIMER_RIGHT_19=2042,23
PRIMER_LEFT_19_TM=59.137
PRIMER_RIGHT_19_TM=60.018
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=531
=