PRIMER_SEQUENCE_ID=NM_002037
SEQUENCE=GCCGCGCTGGTGGCGGCGGCGCGTCGTTGCAGTTGCGCCATCTGTCAGGAGCGGAGCCGGCGAGGAGGGGGCTGCCGCGGGCGAGGAGGAGGGGTCGCCGCGAGCCGAAGGCCTTCGAGACCCGCCCGCCGCCCGGCGGCGAGAGTAGAGGCGAGGTTGTTGTGCGAGCGGCGCGTCCTCTCCCGCCCGGGCGCGCCGCGCTTCTCCCAGCGCACCGAGGACCGCCCGGGCGCACACAAAGCCGCCGCCCGCGCCGCACCGCCCGGCGGCCGCCGCCCGCGCCAGGGAGGGATTCGGCCGCCGGGCCGGGGACACCCCGGCGCCGCCCCCTCGGTGCTCTCGGAAGGCCCACCGGCTCCCGGGCCCGCCGGGGACCCCCCGGAGCCGCCTCGGCCGCGCCGGAGGAGGGCGGGGAGAGGACCATGTGAGTGGGCTCCGGAGCCTCAGCGCCGCGCAGTTTTTTTGAAGAAGCAGGATGCTGATCTAAACGTGGAAAAAGACCAGTCCTGCCTCTGTTGTAGAAGACATGTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAACAAAACTGACGGAGGAGAGGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAGCTTCGGTGTGACCTCCATCCCCAACTACAACAACTTCCACGCAGCCGGGGGCCAAGGACTCACCGTCTTTGGAGGTGTGAACTCTTCGTCTCATACGGGGACCTTGCGTACGAGAGGAGGAACAGGAGTGACACTCTTTGTGGCCCTTTATGACTATGAAGCACGGACAGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAATATTGAACAGCTCGGAAGGAGATTGGTGGGAAGCCCGCTCCTTGACAACTGGAGAGACAGGTTACATTCCCAGCAATTATGTGGCTCCAGTTGACTCTATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGAAAAGATGCTGAGCGACAGCTATTGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGTGAAACCACCAAAGGTGCCTATTCACTTTCTATCCGTGATTGGGATGATATGAAAGGAGACCATGTCAAACATTATAAAATTCGCAAACTTGACAATGGTGGATACTACATTACCACCCGGGCCCAGTTTGAAACACTTCAGCAGCTTGTACAACATTACTCAGAGAGAGCTGCAGGTCTCTGCTGCCGCCTAGTAGTTCCCTGTCACAAAGGGATGCCAAGGCTTACCGATCTGTCTGTCAAAACCAAAGATGTCTGGGAAATCCCTCGAGAATCCCTGCAGTTGATCAAGAGACTGGGAAATGGGCAGTTTGGGGAAGTATGGATGGGTACCTGGAATGGAAACACAAAAGTAGCCATAAAGACTCTTAAACCAGGCACAATGTCCCCCGAATCATTCCTTGAGGAAGCGCAGATCATGAAGAAGCTGAAGCACGACAAGCTGGTCCAGCTCTATGCAGTGGTGTCTGAGGAGCCCATCTACATCGTCACCGAGTATATGAACAAAGGAAGTTTACTGGATTTCTTAAAAGATGGAGAAGGAAGAGCTCTGAAATTACCAAATCTTGTGGACATGGCAGCACAGGTGGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCATAGAGATCTGCGATCAGCAAACATTCTAGTGGGGAATGGACTCATATGCAAGATTGCTGACTTCGGATTGGCCCGATTGATAGAAGACAATGAGTACACAGCAAGACAAGGTGCAAAGTTCCCCATCAAGTGGACGGCCCCCGAGGCAGCCCTGTACGGGAGGTTCACAATCAAGTCTGACGTGTGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAGAGTGCCATACCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGCGAGGCTACAGGATGCCCTGCCCGCAGGACTGCCCCATCTCTCTGCATGAGCTCATGATCCACTGCTGGAAAAAGGACCCTGAAGAACGCCCCACTTTTGAGTACTTGCAGAGCTTCCTGGAAGACTACTTTACCGCGACAGAGCCCCAGTACCAACCTGGTGAAAACCTGTAAGGCCCGGGTCTGCGGAGAGAGGCCTTGTCCCAGAGGCTGCCCCACCCCTCCCCATTAGCTTTCAATTCCGTAGCCAGCTGCTCCCCAGCAGCGGAACCGCCCAGGATCAGATTGCATGTGACTCTGAAGCTGACGAACTTCCATGGCCCTCATTAATGACACTTGTCCCCAAATCCGAACCTCCTCTGTGAAGCATTCGAGACAGAACCTTGTTATTTCTCAGACTTTGGAAAATGCATTGTATCGATGTTATGTAAAAGGCCAAACCTCTGTTCAGTGTAAATAGTTACTCCAGTGCCAACAATCCTAGTGCTTTCCTTTTTTAAAAATGCAAATCCTATGTGATTTTAACTCTGTCTTCACCTGATTCAACTAAAAAAAAAAAAGTATTATTTTCCAAAAGTGGCCTCTTTGTCTAAAACAATAAAATTTTTTTTCATGTTTTAACAAAAACCAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 30286, GC content failed 2964, low tm 10652, high tm 10446, high any compl 5, high end compl 18, high repeat similarity 33, long poly-x seq 47, ok 6121
PRIMER_RIGHT_EXPLAIN=considered 30225, GC content failed 3049, low tm 10858, high tm 10052, high any compl 1, high end compl 7, high repeat similarity 14, long poly-x seq 57, ok 6187
PRIMER_PAIR_EXPLAIN=considered 1011, unacceptable product size 942, high end compl 9, ok 60
PRIMER_PAIR_PENALTY=0.0262
PRIMER_LEFT_PENALTY=0.000148
PRIMER_RIGHT_PENALTY=0.026007
PRIMER_LEFT_SEQUENCE=GAAGAAGCAGGATGCTGATCTAA
PRIMER_RIGHT_SEQUENCE=CTCTTCTGCCTGGATAGAGTCAA
PRIMER_LEFT=464,23
PRIMER_RIGHT=1022,23
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=559
PRIMER_PAIR_PENALTY_1=0.0332
PRIMER_LEFT_1_PENALTY=0.006308
PRIMER_RIGHT_1_PENALTY=0.026932
PRIMER_LEFT_1_SEQUENCE=ATTACCAAATCTTGTGGACATGG
PRIMER_RIGHT_1_SEQUENCE=CTCATTGTCTTCTATCAATCGGG
PRIMER_LEFT_1=1667,23
PRIMER_RIGHT_1=1835,23
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.0370
PRIMER_LEFT_2_PENALTY=0.006308
PRIMER_RIGHT_2_PENALTY=0.030702
PRIMER_LEFT_2_SEQUENCE=ATTACCAAATCTTGTGGACATGG
PRIMER_RIGHT_2_SEQUENCE=GTCAGACTTGATTGTGAACCTCC
PRIMER_LEFT_2=1667,23
PRIMER_RIGHT_2=1922,23
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.0451
PRIMER_LEFT_3_PENALTY=0.006308
PRIMER_RIGHT_3_PENALTY=0.038744
PRIMER_LEFT_3_SEQUENCE=ATTACCAAATCTTGTGGACATGG
PRIMER_RIGHT_3_SEQUENCE=TCTGTCGCGGTAAAGTAGTCTTC
PRIMER_LEFT_3=1667,23
PRIMER_RIGHT_3=2161,23
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=59.961
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=495
PRIMER_PAIR_PENALTY_4=0.0529
PRIMER_LEFT_4_PENALTY=0.026007
PRIMER_RIGHT_4_PENALTY=0.026932
PRIMER_LEFT_4_SEQUENCE=TTGACTCTATCCAGGCAGAAGAG
PRIMER_RIGHT_4_SEQUENCE=CTCATTGTCTTCTATCAATCGGG
PRIMER_LEFT_4=1000,23
PRIMER_RIGHT_4=1835,23
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=59.973
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=836
PRIMER_PAIR_PENALTY_5=0.0567
PRIMER_LEFT_5_PENALTY=0.026007
PRIMER_RIGHT_5_PENALTY=0.030702
PRIMER_LEFT_5_SEQUENCE=TTGACTCTATCCAGGCAGAAGAG
PRIMER_RIGHT_5_SEQUENCE=GTCAGACTTGATTGTGAACCTCC
PRIMER_LEFT_5=1000,23
PRIMER_RIGHT_5=1922,23
PRIMER_LEFT_5_TM=60.026
PRIMER_RIGHT_5_TM=60.031
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=923
PRIMER_PAIR_PENALTY_6=0.0576
PRIMER_LEFT_6_PENALTY=0.026932
PRIMER_RIGHT_6_PENALTY=0.030702
PRIMER_LEFT_6_SEQUENCE=CCCGATTGATAGAAGACAATGAG
PRIMER_RIGHT_6_SEQUENCE=GTCAGACTTGATTGTGAACCTCC
PRIMER_LEFT_6=1813,23
PRIMER_RIGHT_6=1922,23
PRIMER_LEFT_6_TM=59.973
PRIMER_RIGHT_6_TM=60.031
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=110
PRIMER_PAIR_PENALTY_7=0.0657
PRIMER_LEFT_7_PENALTY=0.026932
PRIMER_RIGHT_7_PENALTY=0.038744
PRIMER_LEFT_7_SEQUENCE=CCCGATTGATAGAAGACAATGAG
PRIMER_RIGHT_7_SEQUENCE=TCTGTCGCGGTAAAGTAGTCTTC
PRIMER_LEFT_7=1813,23
PRIMER_RIGHT_7=2161,23
PRIMER_LEFT_7_TM=59.973
PRIMER_RIGHT_7_TM=59.961
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=349
PRIMER_PAIR_PENALTY_8=0.0861
PRIMER_LEFT_8_PENALTY=0.006308
PRIMER_RIGHT_8_PENALTY=0.079770
PRIMER_LEFT_8_SEQUENCE=ATTACCAAATCTTGTGGACATGG
PRIMER_RIGHT_8_SEQUENCE=GTAAGATTCCAAAAGACCACACG
PRIMER_LEFT_8=1667,23
PRIMER_RIGHT_8=1944,23
PRIMER_LEFT_8_TM=59.994
PRIMER_RIGHT_8_TM=59.920
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=8.1000
PRIMER_PAIR_8_COMPL_ANY=7.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=278
PRIMER_PAIR_PENALTY_9=0.0948
PRIMER_LEFT_9_PENALTY=0.006308
PRIMER_RIGHT_9_PENALTY=0.088501
PRIMER_LEFT_9_SEQUENCE=ATTACCAAATCTTGTGGACATGG
PRIMER_RIGHT_9_SEQUENCE=ACGTCAGACTTGATTGTGAACCT
PRIMER_LEFT_9=1667,23
PRIMER_RIGHT_9=1924,23
PRIMER_LEFT_9_TM=59.994
PRIMER_RIGHT_9_TM=60.089
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=258
PRIMER_PAIR_PENALTY_10=0.1026
PRIMER_LEFT_10_PENALTY=0.000148
PRIMER_RIGHT_10_PENALTY=0.102490
PRIMER_LEFT_10_SEQUENCE=GAAGAAGCAGGATGCTGATCTAA
PRIMER_RIGHT_10_SEQUENCE=TACAAGCTGCTGAAGTGTTTCAA
PRIMER_LEFT_10=464,23
PRIMER_RIGHT_10=1262,23
PRIMER_LEFT_10_TM=60.000
PRIMER_RIGHT_10_TM=60.102
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=6.0000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=799
PRIMER_PAIR_PENALTY_11=0.1058
PRIMER_LEFT_11_PENALTY=0.026007
PRIMER_RIGHT_11_PENALTY=0.079770
PRIMER_LEFT_11_SEQUENCE=TTGACTCTATCCAGGCAGAAGAG
PRIMER_RIGHT_11_SEQUENCE=GTAAGATTCCAAAAGACCACACG
PRIMER_LEFT_11=1000,23
PRIMER_RIGHT_11=1944,23
PRIMER_LEFT_11_TM=60.026
PRIMER_RIGHT_11_TM=59.920
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=945
PRIMER_PAIR_PENALTY_12=0.1067
PRIMER_LEFT_12_PENALTY=0.026932
PRIMER_RIGHT_12_PENALTY=0.079770
PRIMER_LEFT_12_SEQUENCE=CCCGATTGATAGAAGACAATGAG
PRIMER_RIGHT_12_SEQUENCE=GTAAGATTCCAAAAGACCACACG
PRIMER_LEFT_12=1813,23
PRIMER_RIGHT_12=1944,23
PRIMER_LEFT_12_TM=59.973
PRIMER_RIGHT_12_TM=59.920
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=132
PRIMER_PAIR_PENALTY_13=0.1145
PRIMER_LEFT_13_PENALTY=0.026007
PRIMER_RIGHT_13_PENALTY=0.088501
PRIMER_LEFT_13_SEQUENCE=TTGACTCTATCCAGGCAGAAGAG
PRIMER_RIGHT_13_SEQUENCE=ACGTCAGACTTGATTGTGAACCT
PRIMER_LEFT_13=1000,23
PRIMER_RIGHT_13=1924,23
PRIMER_LEFT_13_TM=60.026
PRIMER_RIGHT_13_TM=60.089
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=925
PRIMER_PAIR_PENALTY_14=0.1154
PRIMER_LEFT_14_PENALTY=0.026932
PRIMER_RIGHT_14_PENALTY=0.088501
PRIMER_LEFT_14_SEQUENCE=CCCGATTGATAGAAGACAATGAG
PRIMER_RIGHT_14_SEQUENCE=ACGTCAGACTTGATTGTGAACCT
PRIMER_LEFT_14=1813,23
PRIMER_RIGHT_14=1924,23
PRIMER_LEFT_14_TM=59.973
PRIMER_RIGHT_14_TM=60.089
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.6000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=112
PRIMER_PAIR_PENALTY_15=0.1154
PRIMER_LEFT_15_PENALTY=0.026932
PRIMER_RIGHT_15_PENALTY=0.088501
PRIMER_LEFT_15_SEQUENCE=CCCGATTGATAGAAGACAATGAG
PRIMER_RIGHT_15_SEQUENCE=AAGACCACACGTCAGACTTGATT
PRIMER_LEFT_15=1813,23
PRIMER_RIGHT_15=1932,23
PRIMER_LEFT_15_TM=59.973
PRIMER_RIGHT_15_TM=60.089
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=120
PRIMER_PAIR_PENALTY_16=0.1272
PRIMER_LEFT_16_PENALTY=0.088501
PRIMER_RIGHT_16_PENALTY=0.038744
PRIMER_LEFT_16_SEQUENCE=ATCAAGTCTGACGTGTGGTCTTT
PRIMER_RIGHT_16_SEQUENCE=TCTGTCGCGGTAAAGTAGTCTTC
PRIMER_LEFT_16=1911,23
PRIMER_RIGHT_16=2161,23
PRIMER_LEFT_16_TM=60.089
PRIMER_RIGHT_16_TM=59.961
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=251
PRIMER_PAIR_PENALTY_17=0.1272
PRIMER_LEFT_17_PENALTY=0.088501
PRIMER_RIGHT_17_PENALTY=0.038744
PRIMER_LEFT_17_SEQUENCE=AATCAAGTCTGACGTGTGGTCTT
PRIMER_RIGHT_17_SEQUENCE=TCTGTCGCGGTAAAGTAGTCTTC
PRIMER_LEFT_17=1910,23
PRIMER_RIGHT_17=2161,23
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=59.961
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=252
PRIMER_PAIR_PENALTY_18=0.1275
PRIMER_LEFT_18_PENALTY=0.100593
PRIMER_RIGHT_18_PENALTY=0.026932
PRIMER_LEFT_18_SEQUENCE=GTTGACTCTATCCAGGCAGAAGA
PRIMER_RIGHT_18_SEQUENCE=CTCATTGTCTTCTATCAATCGGG
PRIMER_LEFT_18=999,23
PRIMER_RIGHT_18=1835,23
PRIMER_LEFT_18_TM=59.899
PRIMER_RIGHT_18_TM=59.973
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=11.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=837
PRIMER_PAIR_PENALTY_19=0.1285
PRIMER_LEFT_19_PENALTY=0.026007
PRIMER_RIGHT_19_PENALTY=0.102490
PRIMER_LEFT_19_SEQUENCE=TTGACTCTATCCAGGCAGAAGAG
PRIMER_RIGHT_19_SEQUENCE=TACAAGCTGCTGAAGTGTTTCAA
PRIMER_LEFT_19=1000,23
PRIMER_RIGHT_19=1262,23
PRIMER_LEFT_19_TM=60.026
PRIMER_RIGHT_19_TM=60.102
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=263
=