PRIMER_SEQUENCE_ID=NM_002227
SEQUENCE=TCCAGTTTGCTTCTTGGAGAACACTGGACAGCTGAATAAATGCAGTATCTAAATATAAAAGAGGACTGCAATGCCATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGAGGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAATCGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGATGAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCTGTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATGGCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGACAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGGAAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTGATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAACGTTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGAGGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAAACAAGATCCGGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACTCACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATGGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGTAGATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCGACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGTCATGGTCCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAGCGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACATCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAGAAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCTGCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACCTCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTACTAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTTTCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGCACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGAAGGAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACCCCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGCATGATGAGACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGTCCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTCTGGATCTCTTCATGCACCGGAAAAGTGATGTCCTTACCACACCATGGAAATTCAAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGAATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCCGGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATCCCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCCGCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCCATCATGAGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCCAGAAAAAAAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAGAAGCGCTTCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTGCAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTGTTAAATCTCTGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAATCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTACAAAGGAATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATTTCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAATAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGACTATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGTCCTTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCAAAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATTTTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGCTGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATAGGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAAAGAAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAGCTTATGAGAAAATGCTGGGAATTCCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGAAGCATGAATAACATTTAAATTCCACAGATTATCAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 40447, GC content failed 301, low tm 17100, high tm 13427, high any compl 3, high end compl 19, high repeat similarity 33, long poly-x seq 110, ok 9454
PRIMER_RIGHT_EXPLAIN=considered 40439, GC content failed 315, low tm 17231, high tm 13286, high any compl 2, high end compl 1, high repeat similarity 10, long poly-x seq 110, ok 9484
PRIMER_PAIR_EXPLAIN=considered 649, unacceptable product size 587, high end compl 10, ok 52
PRIMER_PAIR_PENALTY=0.0238
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=TTTGCTCAGGGACAGTATGATTT
PRIMER_RIGHT_SEQUENCE=AATGTGAGTGATTTCAGGGAAGA
PRIMER_LEFT=513,23
PRIMER_RIGHT=1163,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=651
PRIMER_PAIR_PENALTY_1=0.0246
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.010384
PRIMER_LEFT_1_SEQUENCE=TTTGCTCAGGGACAGTATGATTT
PRIMER_RIGHT_1_SEQUENCE=TTCCAAGGTAGCCAAGTATTTCA
PRIMER_LEFT_1=513,23
PRIMER_RIGHT_1=863,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=351
PRIMER_PAIR_PENALTY_2=0.0435
PRIMER_LEFT_2_PENALTY=0.008825
PRIMER_RIGHT_2_PENALTY=0.034654
PRIMER_LEFT_2_SEQUENCE=ATCCTCAAAGTCTTAGACCCCAG
PRIMER_RIGHT_2_SEQUENCE=TCAGGCTTCAGAGATTTAACAGC
PRIMER_LEFT_2=1896,23
PRIMER_RIGHT_2=2776,23
PRIMER_LEFT_2_TM=60.009
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=881
PRIMER_PAIR_PENALTY_3=0.0441
PRIMER_LEFT_3_PENALTY=0.034654
PRIMER_RIGHT_3_PENALTY=0.009437
PRIMER_LEFT_3_SEQUENCE=GCTGTTAAATCTCTGAAGCCTGA
PRIMER_RIGHT_3_SEQUENCE=TAAACCGAAGTCTCCAATTTTCA
PRIMER_LEFT_3=2754,23
PRIMER_RIGHT_3=3110,23
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.991
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=357
PRIMER_PAIR_PENALTY_4=0.0443
PRIMER_LEFT_4_PENALTY=0.008825
PRIMER_RIGHT_4_PENALTY=0.035468
PRIMER_LEFT_4_SEQUENCE=ATCCTCAAAGTCTTAGACCCCAG
PRIMER_RIGHT_4_SEQUENCE=ATTTCCATGGACCAGGTCTTTAT
PRIMER_LEFT_4=1896,23
PRIMER_RIGHT_4=2180,23
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=59.965
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=285
PRIMER_PAIR_PENALTY_5=0.0701
PRIMER_LEFT_5_PENALTY=0.035468
PRIMER_RIGHT_5_PENALTY=0.034654
PRIMER_LEFT_5_SEQUENCE=ATAAAGACCTGGTCCATGGAAAT
PRIMER_RIGHT_5_SEQUENCE=TCAGGCTTCAGAGATTTAACAGC
PRIMER_LEFT_5=2158,23
PRIMER_RIGHT_5=2776,23
PRIMER_LEFT_5_TM=59.965
PRIMER_RIGHT_5_TM=60.035
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=619
PRIMER_PAIR_PENALTY_6=0.0738
PRIMER_LEFT_6_PENALTY=0.010384
PRIMER_RIGHT_6_PENALTY=0.063381
PRIMER_LEFT_6_SEQUENCE=TGAAATACTTGGCTACCTTGGAA
PRIMER_RIGHT_6_SEQUENCE=TGATTTCAGGGAAGAATGAAAAA
PRIMER_LEFT_6=841,23
PRIMER_RIGHT_6=1155,23
PRIMER_LEFT_6_TM=60.010
PRIMER_RIGHT_6_TM=59.937
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=30.435
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=7.6000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=315
PRIMER_PAIR_PENALTY_7=0.0767
PRIMER_LEFT_7_PENALTY=0.008825
PRIMER_RIGHT_7_PENALTY=0.067837
PRIMER_LEFT_7_SEQUENCE=ATCCTCAAAGTCTTAGACCCCAG
PRIMER_RIGHT_7_SEQUENCE=CTCTTTCTCAATCAGCGTCTTGT
PRIMER_LEFT_7=1896,23
PRIMER_RIGHT_7=2447,23
PRIMER_LEFT_7_TM=60.009
PRIMER_RIGHT_7_TM=60.068
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=7.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=552
PRIMER_PAIR_PENALTY_8=0.0767
PRIMER_LEFT_8_PENALTY=0.008825
PRIMER_RIGHT_8_PENALTY=0.067837
PRIMER_LEFT_8_SEQUENCE=ATCCTCAAAGTCTTAGACCCCAG
PRIMER_RIGHT_8_SEQUENCE=CAGCTATGTGGTTACCTCCACTC
PRIMER_LEFT_8=1896,23
PRIMER_RIGHT_8=2799,23
PRIMER_LEFT_8_TM=60.009
PRIMER_RIGHT_8_TM=60.068
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=904
PRIMER_PAIR_PENALTY_9=0.0767
PRIMER_LEFT_9_PENALTY=0.008825
PRIMER_RIGHT_9_PENALTY=0.067837
PRIMER_LEFT_9_SEQUENCE=ATCCTCAAAGTCTTAGACCCCAG
PRIMER_RIGHT_9_SEQUENCE=TCAGCTATGTGGTTACCTCCACT
PRIMER_LEFT_9=1896,23
PRIMER_RIGHT_9=2800,23
PRIMER_LEFT_9_TM=60.009
PRIMER_RIGHT_9_TM=60.068
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=905
PRIMER_PAIR_PENALTY_10=0.0773
PRIMER_LEFT_10_PENALTY=0.067837
PRIMER_RIGHT_10_PENALTY=0.009437
PRIMER_LEFT_10_SEQUENCE=AGACAAGACGCTGATTGAGAAAG
PRIMER_RIGHT_10_SEQUENCE=TAAACCGAAGTCTCCAATTTTCA
PRIMER_LEFT_10=2423,23
PRIMER_RIGHT_10=3110,23
PRIMER_LEFT_10_TM=60.068
PRIMER_RIGHT_10_TM=59.991
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=34.783
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=688
PRIMER_PAIR_PENALTY_11=0.0773
PRIMER_LEFT_11_PENALTY=0.067837
PRIMER_RIGHT_11_PENALTY=0.009437
PRIMER_LEFT_11_SEQUENCE=ACAAGACGCTGATTGAGAAAGAG
PRIMER_RIGHT_11_SEQUENCE=TAAACCGAAGTCTCCAATTTTCA
PRIMER_LEFT_11=2425,23
PRIMER_RIGHT_11=3110,23
PRIMER_LEFT_11_TM=60.068
PRIMER_RIGHT_11_TM=59.991
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=7.3000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=686
PRIMER_PAIR_PENALTY_12=0.0773
PRIMER_LEFT_12_PENALTY=0.067837
PRIMER_RIGHT_12_PENALTY=0.009437
PRIMER_LEFT_12_SEQUENCE=GAGTGGAGGTAACCACATAGCTG
PRIMER_RIGHT_12_SEQUENCE=TAAACCGAAGTCTCCAATTTTCA
PRIMER_LEFT_12=2777,23
PRIMER_RIGHT_12=3110,23
PRIMER_LEFT_12_TM=60.068
PRIMER_RIGHT_12_TM=59.991
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=334
PRIMER_PAIR_PENALTY_13=0.0773
PRIMER_LEFT_13_PENALTY=0.067837
PRIMER_RIGHT_13_PENALTY=0.009437
PRIMER_LEFT_13_SEQUENCE=AGTGGAGGTAACCACATAGCTGA
PRIMER_RIGHT_13_SEQUENCE=TAAACCGAAGTCTCCAATTTTCA
PRIMER_LEFT_13=2778,23
PRIMER_RIGHT_13=3110,23
PRIMER_LEFT_13_TM=60.068
PRIMER_RIGHT_13_TM=59.991
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=333
PRIMER_PAIR_PENALTY_14=0.0776
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.063381
PRIMER_LEFT_14_SEQUENCE=TTTGCTCAGGGACAGTATGATTT
PRIMER_RIGHT_14_SEQUENCE=TGATTTCAGGGAAGAATGAAAAA
PRIMER_LEFT_14=513,23
PRIMER_RIGHT_14=1155,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=59.937
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=30.435
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=643
PRIMER_PAIR_PENALTY_15=0.1025
PRIMER_LEFT_15_PENALTY=0.067837
PRIMER_RIGHT_15_PENALTY=0.034654
PRIMER_LEFT_15_SEQUENCE=AGACAAGACGCTGATTGAGAAAG
PRIMER_RIGHT_15_SEQUENCE=TCAGGCTTCAGAGATTTAACAGC
PRIMER_LEFT_15=2423,23
PRIMER_RIGHT_15=2776,23
PRIMER_LEFT_15_TM=60.068
PRIMER_RIGHT_15_TM=60.035
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=354
PRIMER_PAIR_PENALTY_16=0.1025
PRIMER_LEFT_16_PENALTY=0.067837
PRIMER_RIGHT_16_PENALTY=0.034654
PRIMER_LEFT_16_SEQUENCE=ACAAGACGCTGATTGAGAAAGAG
PRIMER_RIGHT_16_SEQUENCE=TCAGGCTTCAGAGATTTAACAGC
PRIMER_LEFT_16=2425,23
PRIMER_RIGHT_16=2776,23
PRIMER_LEFT_16_TM=60.068
PRIMER_RIGHT_16_TM=60.035
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=352
PRIMER_PAIR_PENALTY_17=0.1033
PRIMER_LEFT_17_PENALTY=0.035468
PRIMER_RIGHT_17_PENALTY=0.067837
PRIMER_LEFT_17_SEQUENCE=ATAAAGACCTGGTCCATGGAAAT
PRIMER_RIGHT_17_SEQUENCE=CAGCTATGTGGTTACCTCCACTC
PRIMER_LEFT_17=2158,23
PRIMER_RIGHT_17=2799,23
PRIMER_LEFT_17_TM=59.965
PRIMER_RIGHT_17_TM=60.068
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=642
PRIMER_PAIR_PENALTY_18=0.1033
PRIMER_LEFT_18_PENALTY=0.035468
PRIMER_RIGHT_18_PENALTY=0.067837
PRIMER_LEFT_18_SEQUENCE=ATAAAGACCTGGTCCATGGAAAT
PRIMER_RIGHT_18_SEQUENCE=CTCTTTCTCAATCAGCGTCTTGT
PRIMER_LEFT_18=2158,23
PRIMER_RIGHT_18=2447,23
PRIMER_LEFT_18_TM=59.965
PRIMER_RIGHT_18_TM=60.068
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=8.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=290
PRIMER_PAIR_PENALTY_19=0.1033
PRIMER_LEFT_19_PENALTY=0.035468
PRIMER_RIGHT_19_PENALTY=0.067837
PRIMER_LEFT_19_SEQUENCE=ATAAAGACCTGGTCCATGGAAAT
PRIMER_RIGHT_19_SEQUENCE=TCAGCTATGTGGTTACCTCCACT
PRIMER_LEFT_19=2158,23
PRIMER_RIGHT_19=2800,23
PRIMER_LEFT_19_TM=59.965
PRIMER_RIGHT_19_TM=60.068
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=643
=