PRIMER_SEQUENCE_ID=NM_002350
SEQUENCE=TCGGCCGAGCCCAGAGACAGCCAGTTCCTCTCCCGCCGCGCCGGGCCGCGTGCCGCTCGCTCCCCGGCCGTGGCGCCTCCGGGCCAGACGCGCTGCAGCCTCCAGCCCGCGGCAAGCGGGCGGGGCGGCCGCGCCACCCCCGGCCCCGCGCCAGCAGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAGCCTCCCCATACGCAGTCCTGCTGGACCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGTCACCGTGGAGCTCCGCCGCCCCGAAACTTTCACGCGAGCGGGAAATATGGGATGTATAAAATCAAAAGGGAAAGACAGCTTGAGTGACGATGGAGTAGATTTGAAGACTCAACCAGTACGTAATACTGAAAGAACTATTTATGTGAGAGATCCAACGTCCAATAAACAGCAAAGGCCAGTTCCAGAATCTCAGCTTTTACCTGGACAGAGGTTTCAAACTAAAGATCCAGAGGAACAAGGAGACATTGTGGTAGCCTTGTACCCCTATGATGGCATCCACCCGGACGACTTGTCTTTCAAGAAAGGAGAGAAGATGAAAGTCCTGGAGGAGCATGGAGAATGGTGGAAAGCAAAGTCCCTTTTAACAAAAAAAGAAGGCTTCATCCCCAGCAACTATGTGGCCAAACTCAACACCTTAGAAACAGAAGAGTGGTTTTTCAAGGATATAACCAGGAAGGACGCAGAAAGGCAGCTTTTGGCACCAGGAAATAGCGCTGGAGCTTTCCTTATTAGAGAAAGTGAAACATTAAAAGGAAGCTTCTCTCTGTCTGTCAGAGACTTTGACCCTGTGCATGGTGATGTTATTAAGCACTACAAAATTAGAAGTCTGGATAATGGGGGCTATTACATCTCTCCACGAATCACTTTTCCCTGTATCAGCGACATGATTAAACATTACCAAAAGCAGGCAGATGGCTTGTGCAGAAGATTGGAGAAGGCTTGTATTAGTCCCAAGCCACAGAAGCCATGGGATAAAGATGCCTGGGAGATCCCCCGGGAGTCCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGGGAAGTCTGGATGGGTTACTATAACAACAGTACCAAGGTGGCTGTGAAAACCCTGAAGCCAGGAACTATGTCTGTGCAAGCCTTCCTGGAAGAAGCCAACCTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGCTCTACGCTGTGGTCACCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGGCCAAGGGCAGTTTGCTGGATTTCCTGAAGAGCGATGAAGGTGGCAAAGTGCTGCTTCCAAAGCTCATTGACTTTTCTGCTCAGATTGCAGAGGGAATGGCATACATCGAGCGGAAGAACTACATTCACCGGGACCTGCGAGCAGCTAATGTTCTGGTCTCCGAGTCACTAATGTGCAAAATTGCAGATTTTGGCCTTGCTAGAGTAATTGAAGATAATGAGTACACAGCAAGGGAAGGTGCTAAGTTCCCTATTAAGTGGACGGCTCCAGAAGCAATCAACTTTGGATGTTTCACTATTAAGTCTGATGTGTGGTCCTTTGGAATCCTCCTATACGAAATTGTCACCTATGGGAAAATTCCCTACCCAGGGAGAACTAATGCCGACGTGATGACCGCCCTGTCCCAGGGCTACAGGATGCCCCGTGTGGAGAACTGCCCAGATGAGCTCTATGACATTATGAAAATGTGCTGGAAAGAAAAGGCAGAAGAGAGACCAACGTTTGACTACTTACAGAGCGTCCTGGATGATTTCTACACAGCCACGGAAGGGCAATACCAGCAGCAGCCTTAGAGCACAGGGAGACCCGTCCATTTGGCAGGGGTGGCTGCCTCATTTAGAGAGGAAAAGTAACCATCACTGGTTGCACTTATGATTTCATGTGCGGGGATCATCTGCCGTGCCTGGATCCTGAAATAGAGGCTAAATTACTCAGGAAGAACACCCTCTAAATGGGAAAGTATTCTGTACTCTTAGATGGATTCTCCACTCAGTTGCAACTTGGACTTGTCCTCAGCAGCTGGTAATCTTGCTCTGCTTGACAACATCTGAGTGCAGCCGTTTGAGAAGAAAACATCTATTCTCTCCAAAAATGCACCCAACTAGCTCTATGTTTACAAATGGACATAGGACTCAAAGTTTCAGAGACCATTGCAATGAATCCCCAATAATTGCAGAACTAAACTCATTTATAAAGCTAAAATAACCGGATATATACATAGCATGACATTTCTTTGTGCTTTGGCTTACTTGTTT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 26091, GC content failed 1566, low tm 11083, high tm 7738, high any compl 7, high end compl 25, high repeat similarity 17, long poly-x seq 40, ok 5615
PRIMER_RIGHT_EXPLAIN=considered 26120, GC content failed 1067, low tm 11744, high tm 7520, high any compl 1, high end compl 2, high repeat similarity 1, long poly-x seq 36, ok 5749
PRIMER_PAIR_EXPLAIN=considered 268, unacceptable product size 210, high any compl 2, high end compl 3, ok 53
PRIMER_PAIR_PENALTY=0.0294
PRIMER_LEFT_PENALTY=0.021648
PRIMER_RIGHT_PENALTY=0.007752
PRIMER_LEFT_SEQUENCE=TTTTACCTGGACAGAGGTTTCAA
PRIMER_RIGHT_SEQUENCE=CTGATACAGGGAAAAGTGATTCG
PRIMER_LEFT=445,23
PRIMER_RIGHT=910,23
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=466
PRIMER_PAIR_PENALTY_1=0.0404
PRIMER_LEFT_1_PENALTY=0.018344
PRIMER_RIGHT_1_PENALTY=0.022025
PRIMER_LEFT_1_SEQUENCE=GTCCTGGATGATTTCTACACAGC
PRIMER_RIGHT_1_SEQUENCE=AGTGCAACCAGTGATGGTTACTT
PRIMER_LEFT_1=1782,23
PRIMER_RIGHT_1=1912,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.0444
PRIMER_LEFT_2_PENALTY=0.026007
PRIMER_RIGHT_2_PENALTY=0.018344
PRIMER_LEFT_2_SEQUENCE=GAGTCCATCAAGTTGGTGAAAAG
PRIMER_RIGHT_2_SEQUENCE=GCTGTGTAGAAATCATCCAGGAC
PRIMER_LEFT_2=1029,23
PRIMER_RIGHT_2=1804,23
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=776
PRIMER_PAIR_PENALTY_3=0.0477
PRIMER_LEFT_3_PENALTY=0.021648
PRIMER_RIGHT_3_PENALTY=0.026007
PRIMER_LEFT_3_SEQUENCE=TTTTACCTGGACAGAGGTTTCAA
PRIMER_RIGHT_3_SEQUENCE=CTTTTCACCAACTTGATGGACTC
PRIMER_LEFT_3=445,23
PRIMER_RIGHT_3=1051,23
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=607
PRIMER_PAIR_PENALTY_4=0.0480
PRIMER_LEFT_4_PENALTY=0.026007
PRIMER_RIGHT_4_PENALTY=0.022025
PRIMER_LEFT_4_SEQUENCE=GAGTCCATCAAGTTGGTGAAAAG
PRIMER_RIGHT_4_SEQUENCE=AGTGCAACCAGTGATGGTTACTT
PRIMER_LEFT_4=1029,23
PRIMER_RIGHT_4=1912,23
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=884
PRIMER_PAIR_PENALTY_5=0.0482
PRIMER_LEFT_5_PENALTY=0.045027
PRIMER_RIGHT_5_PENALTY=0.003218
PRIMER_LEFT_5_SEQUENCE=GTCTGGATAATGGGGGCTATTAC
PRIMER_RIGHT_5_SEQUENCE=TTTTCTTTCCAGCACATTTTCAT
PRIMER_LEFT_5=856,23
PRIMER_RIGHT_5=1744,23
PRIMER_LEFT_5_TM=59.955
PRIMER_RIGHT_5_TM=59.997
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=30.435
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=6.9000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=889
PRIMER_PAIR_PENALTY_6=0.0615
PRIMER_LEFT_6_PENALTY=0.053769
PRIMER_RIGHT_6_PENALTY=0.007752
PRIMER_LEFT_6_SEQUENCE=GAACAAGGAGACATTGTGGTAGC
PRIMER_RIGHT_6_SEQUENCE=CTGATACAGGGAAAAGTGATTCG
PRIMER_LEFT_6=483,23
PRIMER_RIGHT_6=910,23
PRIMER_LEFT_6_TM=60.054
PRIMER_RIGHT_6_TM=60.008
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MER21 a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=8.6000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=428
PRIMER_PAIR_PENALTY_7=0.0634
PRIMER_LEFT_7_PENALTY=0.045027
PRIMER_RIGHT_7_PENALTY=0.018344
PRIMER_LEFT_7_SEQUENCE=GTCTGGATAATGGGGGCTATTAC
PRIMER_RIGHT_7_SEQUENCE=GCTGTGTAGAAATCATCCAGGAC
PRIMER_LEFT_7=856,23
PRIMER_RIGHT_7=1804,23
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=60.018
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=949
PRIMER_PAIR_PENALTY_8=0.0647
PRIMER_LEFT_8_PENALTY=0.018344
PRIMER_RIGHT_8_PENALTY=0.046368
PRIMER_LEFT_8_SEQUENCE=GTCCTGGATGATTTCTACACAGC
PRIMER_RIGHT_8_SEQUENCE=TGAAATCATAAGTGCAACCAGTG
PRIMER_LEFT_8=1782,23
PRIMER_RIGHT_8=1922,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=60.046
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=8.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=141
PRIMER_PAIR_PENALTY_9=0.0667
PRIMER_LEFT_9_PENALTY=0.021648
PRIMER_RIGHT_9_PENALTY=0.045027
PRIMER_LEFT_9_SEQUENCE=TTTTACCTGGACAGAGGTTTCAA
PRIMER_RIGHT_9_SEQUENCE=GTAATAGCCCCCATTATCCAGAC
PRIMER_LEFT_9=445,23
PRIMER_RIGHT_9=878,23
PRIMER_LEFT_9_TM=60.022
PRIMER_RIGHT_9_TM=59.955
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MSTa consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.3000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=434
PRIMER_PAIR_PENALTY_10=0.0710
PRIMER_LEFT_10_PENALTY=0.045027
PRIMER_RIGHT_10_PENALTY=0.026007
PRIMER_LEFT_10_SEQUENCE=GTCTGGATAATGGGGGCTATTAC
PRIMER_RIGHT_10_SEQUENCE=CTTTTCACCAACTTGATGGACTC
PRIMER_LEFT_10=856,23
PRIMER_RIGHT_10=1051,23
PRIMER_LEFT_10_TM=59.955
PRIMER_RIGHT_10_TM=60.026
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=196
PRIMER_PAIR_PENALTY_11=0.0724
PRIMER_LEFT_11_PENALTY=0.026007
PRIMER_RIGHT_11_PENALTY=0.046368
PRIMER_LEFT_11_SEQUENCE=GAGTCCATCAAGTTGGTGAAAAG
PRIMER_RIGHT_11_SEQUENCE=TGAAATCATAAGTGCAACCAGTG
PRIMER_LEFT_11=1029,23
PRIMER_RIGHT_11=1922,23
PRIMER_LEFT_11_TM=60.026
PRIMER_RIGHT_11_TM=60.046
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=894
PRIMER_PAIR_PENALTY_12=0.0776
PRIMER_LEFT_12_PENALTY=0.074396
PRIMER_RIGHT_12_PENALTY=0.003218
PRIMER_LEFT_12_SEQUENCE=TGGCCTTGCTAGAGTAATTGAAG
PRIMER_RIGHT_12_SEQUENCE=TTTTCTTTCCAGCACATTTTCAT
PRIMER_LEFT_12=1454,23
PRIMER_RIGHT_12=1744,23
PRIMER_LEFT_12_TM=59.926
PRIMER_RIGHT_12_TM=59.997
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=30.435
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=291
PRIMER_PAIR_PENALTY_13=0.0776
PRIMER_LEFT_13_PENALTY=0.074396
PRIMER_RIGHT_13_PENALTY=0.003218
PRIMER_LEFT_13_SEQUENCE=CAGATTTTGGCCTTGCTAGAGTA
PRIMER_RIGHT_13_SEQUENCE=TTTTCTTTCCAGCACATTTTCAT
PRIMER_LEFT_13=1447,23
PRIMER_RIGHT_13=1744,23
PRIMER_LEFT_13_TM=59.926
PRIMER_RIGHT_13_TM=59.997
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=30.435
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.4000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=298
PRIMER_PAIR_PENALTY_14=0.0798
PRIMER_LEFT_14_PENALTY=0.053769
PRIMER_RIGHT_14_PENALTY=0.026007
PRIMER_LEFT_14_SEQUENCE=GAACAAGGAGACATTGTGGTAGC
PRIMER_RIGHT_14_SEQUENCE=CTTTTCACCAACTTGATGGACTC
PRIMER_LEFT_14=483,23
PRIMER_RIGHT_14=1051,23
PRIMER_LEFT_14_TM=60.054
PRIMER_RIGHT_14_TM=60.026
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=569
PRIMER_PAIR_PENALTY_15=0.0859
PRIMER_LEFT_15_PENALTY=0.018344
PRIMER_RIGHT_15_PENALTY=0.067516
PRIMER_LEFT_15_SEQUENCE=GTCCTGGATGATTTCTACACAGC
PRIMER_RIGHT_15_SEQUENCE=TGTTGTCAAGCAGAGCAAGATTA
PRIMER_LEFT_15=1782,23
PRIMER_RIGHT_15=2088,23
PRIMER_LEFT_15_TM=60.018
PRIMER_RIGHT_15_TM=60.068
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=5.9000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=307
PRIMER_PAIR_PENALTY_16=0.0915
PRIMER_LEFT_16_PENALTY=0.021648
PRIMER_RIGHT_16_PENALTY=0.069862
PRIMER_LEFT_16_SEQUENCE=TTTTACCTGGACAGAGGTTTCAA
PRIMER_RIGHT_16_SEQUENCE=TAATAGCCCCCATTATCCAGACT
PRIMER_LEFT_16=445,23
PRIMER_RIGHT_16=877,23
PRIMER_LEFT_16_TM=60.022
PRIMER_RIGHT_16_TM=60.070
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=433
PRIMER_PAIR_PENALTY_17=0.0920
PRIMER_LEFT_17_PENALTY=0.018344
PRIMER_RIGHT_17_PENALTY=0.073619
PRIMER_LEFT_17_SEQUENCE=GTCCTGGATGATTTCTACACAGC
PRIMER_RIGHT_17_SEQUENCE=GCAACCAGTGATGGTTACTTTTC
PRIMER_LEFT_17=1782,23
PRIMER_RIGHT_17=1909,23
PRIMER_LEFT_17_TM=60.018
PRIMER_RIGHT_17_TM=59.926
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=7.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=128
PRIMER_PAIR_PENALTY_18=0.0927
PRIMER_LEFT_18_PENALTY=0.074396
PRIMER_RIGHT_18_PENALTY=0.018344
PRIMER_LEFT_18_SEQUENCE=TGGCCTTGCTAGAGTAATTGAAG
PRIMER_RIGHT_18_SEQUENCE=GCTGTGTAGAAATCATCCAGGAC
PRIMER_LEFT_18=1454,23
PRIMER_RIGHT_18=1804,23
PRIMER_LEFT_18_TM=59.926
PRIMER_RIGHT_18_TM=60.018
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=351
PRIMER_PAIR_PENALTY_19=0.0927
PRIMER_LEFT_19_PENALTY=0.074396
PRIMER_RIGHT_19_PENALTY=0.018344
PRIMER_LEFT_19_SEQUENCE=CAGATTTTGGCCTTGCTAGAGTA
PRIMER_RIGHT_19_SEQUENCE=GCTGTGTAGAAATCATCCAGGAC
PRIMER_LEFT_19=1447,23
PRIMER_RIGHT_19=1804,23
PRIMER_LEFT_19_TM=59.926
PRIMER_RIGHT_19_TM=60.018
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.4000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=358
=