PRIMER_SEQUENCE_ID=NM_002447
SEQUENCE=GGATCCTCTAGGGTCCCAGCTCGCCTCGATGGAGCTCCTCCCGCCGCTGCCTCAGTCCTTCCTGTTGCTGCTGCTGTTGCCTGCCAAGCCCGCGGCGGGCGAGGACTGGCAGTGCCCGCGCACCCCCTACGCGGCCTCTCGCGACTTTGACGTGAAGTACGTGGTGCCCAGCTTCTCCGCCGGAGGCCTGGTACAGGCCATGGTGACCTACGAGGGCGACAGAAATGAGAGTGCTGTGTTTGTAGCCATACGCAATCGCCTGCATGTGCTTGGGCCTGACCTGAAGTCTGTCCAGAGCCTGGCCACGGGCCCTGCTGGAGACCCTGGCTGCCAGACGTGTGCAGCCTGTGGCCCAGGACCCCACGGCCCTCCCGGTGACACAGACACAAAGGTGCTGGTGCTGGATCCCGCGCTGCCTGCGCTGGTCAGTTGTGGCTCCAGCCTGCAGGGCCGCTGCTTCCTGCATGACCTAGAGCCCCAAGGGACAGCCGTGCATCTGGCAGCGCCAGCCTGCCTCTTCTCAGCCCACCATAACCGGCCCGATGACTGCCCCGACTGTGTGGCCAGCCCATTGGGCACCCGTGTAACTGTGGTTGAGCAAGGCCAGGCCTCCTATTTCTACGTGGCATCCTCACTGGACGCAGCCGTGGCTGGCAGCTTCAGCCCACGCTCAGTGTCTATCAGGCGTCTCAAGGCTGACGCCTCGGGATTCGCACCGGGCTTTGTGGCGTTGTCAGTGCTGCCCAAGCATCTTGTCTCCTACAGTATTGAATACGTGCACAGCTTCCACACGGGAGCCTTCGTATACTTCCTGACTGTACAGCCGGCCAGCGTGACAGATGATCCTAGTGCCCTGCACACACGCCTGGCACGGCTTAGCGCCACTGAGCCAGAGTTGGGTGACTATCGGGAGCTGGTCCTCGACTGCAGATTTGCTCCAAAACGCAGGCGCCGGGGGGCCCCAGAAGGCGGACAGCCCTACCCTGTGCTGCAGGTGGCCCACTCCGCTCCAGTGGGTGCCCAACTTGCCACTGAGCTGAGCATCGCCGAGGGCCAGGAAGTACTATTTGGGGTCTTTGTGACTGGCAAGGATGGTGGTCCTGGCGTGGGCCCCAACTCTGTCGTCTGTGCCTTCCCCATTGACCTGCTGGACACACTAATTGATGAGGGTGTGGAGCGCTGTTGTGAATCCCCAGTCCATCCAGGCCTCCGGCGAGGCCTCGACTTCTTCCAGTCGCCCAGTTTTTGCCCCAACCCGCCTGGCCTGGAAGCCCTCAGCCCCAACACCAGCTGCCGCCACTTCCCTCTGCTGGTCAGTAGCAGCTTCTCACGTGTGGACCTATTCAATGGGCTGTTGGGACCAGTACAGGTCACTGCATTGTATGTGACACGCCTTGACAACGTCACAGTGGCACACATGGGCACAATGGATGGGCGTATCCTGCAGGTGGAGCTGGTCAGGTCACTAAACTACTTGCTGTATGTGTCCAACTTCTCACTGGGTGACAGTGGGCAGCCCGTGCAGCGGGATGTCAGTCGTCTTGGGGACCACCTACTCTTTGCCTCTGGGGACCAGGTTTTCCAGGTACCTATCCGAGGCCCTGGCTGCCGCCACTTCCTGACCTGTGGGCGTTGCCTAAGGGCATGGCATTTCATGGGCTGTGGCTGGTGTGGGAACATGTGCGGCCAGCAGAAGGAGTGTCCTGGCTCCTGGCAACAGGACCACTGCCCACCTAAGCTTACTGAGTTCCACCCCCACAGTGGACCTCTAAGGGGCAGTACAAGGCTGACCCTGTGTGGCTCCAACTTCTACCTTCACCCTTCTGGTCTGGTGCCTGAGGGAACCCATCAGGTCACTGTGGGCCAAAGTCCCTGCCGGCCACTGCCCAAGGACAGCTCAAAACTCAGACCAGTGCCCCGGAAAGACTTTGTAGAGGAGTTTGAGTGTGAACTGGAGCCCTTGGGCACCCAGGCAGTGGGGCCTACCAACGTCAGCCTCACCGTGACTAACATGCCACCGGGCAAGCACTTCCGGGTAGACGGCACCTCCGTGCTGAGAGGCTTCTCTTTCATGGAGCCAGTGCTGATAGCAGTGCAACCCCTCTTTGGCCCACGGGCAGGAGGCACCTGTCTCACTCTTGAAGGCCAGAGTCTGTCTGTAGGCACCAGCCGGGCTGTGCTGGTCAATGGGACTGAGTGTCTGCTAGCACGGGTCAGTGAGGGGCAGCTTTTATGTGCCACACCCCCTGGGGCCACGGTGGCCAGTGTCCCCCTTAGCCTGCAGGTGGGGGGTGCCCAGGTACCTGGTTCCTGGACCTTCCAGTACAGAGAAGACCCTGTCGTGCTAAGCATCAGCCCCAACTGTGGCTACATCAACTCCCACATCACCATCTGTGGCCAGCATCTAACTTCAGCATGGCACTTAGTGCTGTCATTCCATGACGGGCTTAGGGCAGTGGAAAGCAGGTGTGAGAGGCAGCTTCCAGAGCAGCAGCTGTGCCGCCTTCCTGAATATGTGGTCCGAGACCCCCAGGGATGGGTGGCAGGGAATCTGAGTGCCCGAGGGGATGGAGCTGCTGGCTTTACACTGCCTGGCTTTCGCTTCCTACCCCCACCCCATCCACCCAGTGCCAACCTAGTTCCACTGAAGCCTGAGGAGCATGCCATTAAGTTTGAGTATATTGGGCTGGGCGCTGTGGCTGACTGTGTGGGTATCAACGTGACCGTGGGTGGTGAGAGCTGCCAGCACGAGTTCCGGGGGGACATGGTTGTCTGCCCCCTGCCCCCATCCCTGCAGCTTGGCCAGGATGGTGCCCCATTGCAGGTCTGCGTAGATGGTGAATGTCATATCCTGGGTAGAGTGGTGCGGCCAGGGCCAGATGGGGTCCCACAGAGCACGCTCCTTGGTATCCTGCTGCCTTTGCTGCTGCTTGTGGCTGCACTGGCGACTGCACTGGTCTTCAGCTACTGGTGGCGGAGGAAGCAGCTAGTTCTTCCTCCCAACCTGAATGACCTGGCATCCCTGGACCAGACTGCTGGAGCCACACCCCTGCCTATTCTGTACTCGGGCTCTGACTACAGAAGTGGCCTTGCACTCCCTGCCATTGATGGTCTGGATTCCACCACTTGTGTCCATGGAGCATCCTTCTCCGATAGTGAAGATGAATCCTGTGTGCCACTGCTGCGGAAAGAGTCCATCCAGCTAAGGGACCTGGACTCTGCGCTCTTGGCTGAGGTCAAGGATGTGCTGATTCCCCATGAGCGGGTGGTCACCCACAGTGACCGAGTCATTGGCAAAGGCCACTTTGGAGTTGTCTACCACGGAGAATACATAGACCAGGCCCAGAATCGAATCCAATGTGCCATCAAGTCACTAAGTCGCATCACAGAGATGCAGCAGGTGGAGGCCTTCCTGCGAGAGGGGCTGCTCATGCGTGGCCTGAACCACCCGAATGTGCTGGCTCTCATTGGTATCATGTTGCCACCTGAGGGCCTGCCCCATGTGCTGCTGCCCTATATGTGCCACGGTGACCTGCTCCAGTTCATCCGCTCACCTCAGCGGAACCCCACCGTGAAGGACCTCATCAGCTTTGGCCTGCAGGTAGCCCGCGGCATGGAGTACCTGGCAGAGCAGAAGTTTGTGCACAGGGACCTGGCTGCGCGGAACTGCATGCTGGACGAGTCATTCACAGTCAAGGTGGCTGACTTTGGTTTGGCCCGCGACATCCTGGACAGGGAGTACTATAGTGTTCAACAGCATCGCCACGCTCGCCTACCTGTGAAGTGGATGGCGCTGGAGAGCCTGCAGACCTATAGATTTACCACCAAGTCTGATGTGTGGTCATTTGGTGTGCTGCTGTGGGAACTGCTGACACGGGGTGCCCCACCATACCGCCACATTGACCCTTTTGACCTTACCCACTTCCTGGCCCAGGGTCGGCGCCTGCCCCAGCCTGAGTATTGCCCTGATTCTCTGTACCAAGTGATGCAGCAATGCTGGGAGGCAGACCCAGCAGTGCGACCCACCTTCAGAGTACTAGTGGGGGAGGTGGAGCAGATAGTGTCTGCACTGCTTGGGGACCATTATGTGCAGCTGCCAGCAACCTACATGAACTTGGGCCCCAGCACCTCGCATGAGATGAATGTGCGTCCAGAACAGCCGCAGTTCTCACCCATGCCAGGGAATGTACGCCGGCCCCGGCCACTCTCAGAGCCTCCTCGGCCCACTTGACTTAGTTCTTGGGCTGGACCTGCTTAGCTGCCTTGAGCTAACCCCAAGGCTGCCTCTGGGCCATGCCAGGCCAGAGCAGTGGCCCTCCACCTTGTTCCTGCCCTTTAACTTTCAGAGGCAATAGGTAAATGGGCCCATTAGGTCCCTCACTCCACAGAGTGAGCCAGTGAGGGCAGTCCTGCAACATGTATTTATGGAGTGCCTGCTGTGGACCCTGTCTTCTGGGCACAGTGGACTCAGCAGTGACCACACCAACACTGACCCTTGAACCAATAAAGGAACAAATGACTATTAAAGCACAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 52910, GC content failed 869, low tm 12601, high tm 29315, high any compl 11, high end compl 35, high repeat similarity 53, long poly-x seq 57, ok 9969
PRIMER_RIGHT_EXPLAIN=considered 52884, GC content failed 853, low tm 12942, high tm 28902, high any compl 1, high end compl 11, high repeat similarity 10, long poly-x seq 70, ok 10095
PRIMER_PAIR_EXPLAIN=considered 984, unacceptable product size 922, high end compl 3, ok 59
PRIMER_PAIR_PENALTY=0.0684
PRIMER_LEFT_PENALTY=0.042506
PRIMER_RIGHT_PENALTY=0.025930
PRIMER_LEFT_SEQUENCE=CTCCAACTTCTACCTTCACCCTT
PRIMER_RIGHT_SEQUENCE=ACTGGTCTGAGTTTTGAGCTGTC
PRIMER_LEFT=1800,23
PRIMER_RIGHT=1913,23
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MER4B a consensus
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.0816
PRIMER_LEFT_1_PENALTY=0.031944
PRIMER_RIGHT_1_PENALTY=0.049672
PRIMER_LEFT_1_SEQUENCE=CTGCGTAGATGGTGAATGTCATA
PRIMER_RIGHT_1_SEQUENCE=CATCTCTGTGATGCGACTTAGTG
PRIMER_LEFT_1=2826,23
PRIMER_RIGHT_1=3393,23
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=568
PRIMER_PAIR_PENALTY_2=0.0838
PRIMER_LEFT_2_PENALTY=0.057845
PRIMER_RIGHT_2_PENALTY=0.025930
PRIMER_LEFT_2_SEQUENCE=ACCTGCTGGACACACTAATTGAT
PRIMER_RIGHT_2_SEQUENCE=ACTGGTCTGAGTTTTGAGCTGTC
PRIMER_LEFT_2=1142,23
PRIMER_RIGHT_2=1913,23
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.974
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=772
PRIMER_PAIR_PENALTY_3=0.0922
PRIMER_LEFT_3_PENALTY=0.034339
PRIMER_RIGHT_3_PENALTY=0.057845
PRIMER_LEFT_3_SEQUENCE=CAGAAATGAGAGTGCTGTGTTTG
PRIMER_RIGHT_3_SEQUENCE=ATTAGTGTGTCCAGCAGGTCAAT
PRIMER_LEFT_3=219,23
PRIMER_RIGHT_3=1160,23
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.942
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=942
PRIMER_PAIR_PENALTY_4=0.0928
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.049672
PRIMER_LEFT_4_SEQUENCE=GTACTCGGGCTCTGACTACAGAA
PRIMER_RIGHT_4_SEQUENCE=CATCTCTGTGATGCGACTTAGTG
PRIMER_LEFT_4=3060,23
PRIMER_RIGHT_4=3393,23
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=334
PRIMER_PAIR_PENALTY_5=0.0995
PRIMER_LEFT_5_PENALTY=0.043085
PRIMER_RIGHT_5_PENALTY=0.056374
PRIMER_LEFT_5_SEQUENCE=GTACTCGGGCTCTGACTACAGAA
PRIMER_RIGHT_5_SEQUENCE=CACACATCAGACTTGGTGGTAAA
PRIMER_LEFT_5=3060,23
PRIMER_RIGHT_5=3839,23
PRIMER_LEFT_5_TM=59.957
PRIMER_RIGHT_5_TM=59.944
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=780
PRIMER_PAIR_PENALTY_6=0.1004
PRIMER_LEFT_6_PENALTY=0.057845
PRIMER_RIGHT_6_PENALTY=0.042506
PRIMER_LEFT_6_SEQUENCE=ACCTGCTGGACACACTAATTGAT
PRIMER_RIGHT_6_SEQUENCE=AAGGGTGAAGGTAGAAGTTGGAG
PRIMER_LEFT_6=1142,23
PRIMER_RIGHT_6=1822,23
PRIMER_LEFT_6_TM=59.942
PRIMER_RIGHT_6_TM=60.043
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=681
PRIMER_PAIR_PENALTY_7=0.1145
PRIMER_LEFT_7_PENALTY=0.025930
PRIMER_RIGHT_7_PENALTY=0.088543
PRIMER_LEFT_7_SEQUENCE=GACAGCTCAAAACTCAGACCAGT
PRIMER_RIGHT_7_SEQUENCE=CCAGGATATGACATTCACCATCT
PRIMER_LEFT_7=1891,23
PRIMER_RIGHT_7=2854,23
PRIMER_LEFT_7_TM=59.974
PRIMER_RIGHT_7_TM=60.089
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER22 Human SSTI moderately repetitive DNA sequence family
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=964
PRIMER_PAIR_PENALTY_8=0.1314
PRIMER_LEFT_8_PENALTY=0.081736
PRIMER_RIGHT_8_PENALTY=0.049672
PRIMER_LEFT_8_SEQUENCE=TACTCGGGCTCTGACTACAGAAG
PRIMER_RIGHT_8_SEQUENCE=CATCTCTGTGATGCGACTTAGTG
PRIMER_LEFT_8=3061,23
PRIMER_RIGHT_8=3393,23
PRIMER_LEFT_8_TM=60.082
PRIMER_RIGHT_8_TM=59.950
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=333
PRIMER_PAIR_PENALTY_9=0.1361
PRIMER_LEFT_9_PENALTY=0.047559
PRIMER_RIGHT_9_PENALTY=0.088543
PRIMER_LEFT_9_SEQUENCE=ACCTTCCAGTACAGAGAAGACCC
PRIMER_RIGHT_9_SEQUENCE=CCAGGATATGACATTCACCATCT
PRIMER_LEFT_9=2314,23
PRIMER_RIGHT_9=2854,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=60.089
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.1000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=541
PRIMER_PAIR_PENALTY_10=0.1381
PRIMER_LEFT_10_PENALTY=0.081736
PRIMER_RIGHT_10_PENALTY=0.056374
PRIMER_LEFT_10_SEQUENCE=TACTCGGGCTCTGACTACAGAAG
PRIMER_RIGHT_10_SEQUENCE=CACACATCAGACTTGGTGGTAAA
PRIMER_LEFT_10=3061,23
PRIMER_RIGHT_10=3839,23
PRIMER_LEFT_10_TM=60.082
PRIMER_RIGHT_10_TM=59.944
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=779
PRIMER_PAIR_PENALTY_11=0.1423
PRIMER_LEFT_11_PENALTY=0.056374
PRIMER_RIGHT_11_PENALTY=0.085878
PRIMER_LEFT_11_SEQUENCE=TTTACCACCAAGTCTGATGTGTG
PRIMER_RIGHT_11_SEQUENCE=CTTTATTGGTTCAAGGGTCAGTG
PRIMER_LEFT_11=3817,23
PRIMER_RIGHT_11=4508,23
PRIMER_LEFT_11_TM=59.944
PRIMER_RIGHT_11_TM=59.914
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=692
PRIMER_PAIR_PENALTY_12=0.1514
PRIMER_LEFT_12_PENALTY=0.043085
PRIMER_RIGHT_12_PENALTY=0.108280
PRIMER_LEFT_12_SEQUENCE=GTACTCGGGCTCTGACTACAGAA
PRIMER_RIGHT_12_SEQUENCE=ATTCTGGGCCTGGTCTATGTATT
PRIMER_LEFT_12=3060,23
PRIMER_RIGHT_12=3348,23
PRIMER_LEFT_12_TM=59.957
PRIMER_RIGHT_12_TM=60.108
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=289
PRIMER_PAIR_PENALTY_13=0.1581
PRIMER_LEFT_13_PENALTY=0.056374
PRIMER_RIGHT_13_PENALTY=0.101745
PRIMER_LEFT_13_SEQUENCE=TTTACCACCAAGTCTGATGTGTG
PRIMER_RIGHT_13_SEQUENCE=GTTCCTTTATTGGTTCAAGGGTC
PRIMER_LEFT_13=3817,23
PRIMER_RIGHT_13=4512,23
PRIMER_LEFT_13_TM=59.944
PRIMER_RIGHT_13_TM=60.102
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=9.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=696
PRIMER_PAIR_PENALTY_14=0.1631
PRIMER_LEFT_14_PENALTY=0.031944
PRIMER_RIGHT_14_PENALTY=0.131151
PRIMER_LEFT_14_SEQUENCE=CTGCGTAGATGGTGAATGTCATA
PRIMER_RIGHT_14_SEQUENCE=AGTAGCTGAAGACCAGTGCAGTC
PRIMER_LEFT_14=2826,23
PRIMER_RIGHT_14=2968,23
PRIMER_LEFT_14_TM=60.032
PRIMER_RIGHT_14_TM=60.131
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=143
PRIMER_PAIR_PENALTY_15=0.1787
PRIMER_LEFT_15_PENALTY=0.047559
PRIMER_RIGHT_15_PENALTY=0.131151
PRIMER_LEFT_15_SEQUENCE=ACCTTCCAGTACAGAGAAGACCC
PRIMER_RIGHT_15_SEQUENCE=AGTAGCTGAAGACCAGTGCAGTC
PRIMER_LEFT_15=2314,23
PRIMER_RIGHT_15=2968,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=60.131
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.1000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=655
PRIMER_PAIR_PENALTY_16=0.1900
PRIMER_LEFT_16_PENALTY=0.081736
PRIMER_RIGHT_16_PENALTY=0.108280
PRIMER_LEFT_16_SEQUENCE=TACTCGGGCTCTGACTACAGAAG
PRIMER_RIGHT_16_SEQUENCE=ATTCTGGGCCTGGTCTATGTATT
PRIMER_LEFT_16=3061,23
PRIMER_RIGHT_16=3348,23
PRIMER_LEFT_16_TM=60.082
PRIMER_RIGHT_16_TM=60.108
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=288
PRIMER_PAIR_PENALTY_17=0.1989
PRIMER_LEFT_17_PENALTY=0.172940
PRIMER_RIGHT_17_PENALTY=0.025930
PRIMER_LEFT_17_SEQUENCE=CCACCTAAGCTTACTGAGTTCCA
PRIMER_RIGHT_17_SEQUENCE=ACTGGTCTGAGTTTTGAGCTGTC
PRIMER_LEFT_17=1729,23
PRIMER_RIGHT_17=1913,23
PRIMER_LEFT_17_TM=59.827
PRIMER_RIGHT_17_TM=59.974
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=185
PRIMER_PAIR_PENALTY_18=0.2086
PRIMER_LEFT_18_PENALTY=0.182658
PRIMER_RIGHT_18_PENALTY=0.025930
PRIMER_LEFT_18_SEQUENCE=CTGCTGGACACACTAATTGATGA
PRIMER_RIGHT_18_SEQUENCE=ACTGGTCTGAGTTTTGAGCTGTC
PRIMER_LEFT_18=1144,23
PRIMER_RIGHT_18=1913,23
PRIMER_LEFT_18_TM=60.183
PRIMER_RIGHT_18_TM=59.974
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=770
PRIMER_PAIR_PENALTY_19=0.2115
PRIMER_LEFT_19_PENALTY=0.042506
PRIMER_RIGHT_19_PENALTY=0.169021
PRIMER_LEFT_19_SEQUENCE=CTCCAACTTCTACCTTCACCCTT
PRIMER_RIGHT_19_SEQUENCE=CCAGTTCACACTCAAACTCCTCT
PRIMER_LEFT_19=1800,23
PRIMER_RIGHT_19=1954,23
PRIMER_LEFT_19_TM=60.043
PRIMER_RIGHT_19_TM=59.831
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=155
=