PRIMER_SEQUENCE_ID=NM_002530
SEQUENCE=GATGCGAGCCGGCCACCAGTCCCGGCAGAGCCACTAGGGCCTCTCGCGGCTCCCACCCGGCGGCGGCGGCGGCGGCGGCGGCGTCCGCGATGGTTTCAGACGCTGAAGGATTTTGCATCTGATCGCTCGGCGTTTCAAAGAAGCAGCGATCGGAGATGGATGTCTCTCTTTGCCCAGCCAAGTGTAGTTTCTGGCGGATTTTCTTGCTGGGAAGCGTCTGGCTGGACTATGTGGGCTCCGTGCTGGCTTGCCCTGCAAATTGTGTCTGCAGCAAGACTGAGATCAATTGCCGGCGGCCGGACGATGGGAACCTCTTCCCCCTCCTGGAAGGGCAGGATTCAGGGAACAGCAATGGGAACGCCAGTATCAACATCACGGACATCTCAAGGAATATCACTTCCATACACATAGAGAACTGGCGCAGTCTTCACACGCTCAACGCCGTGGACATGGAGCTCTACACCGGACTTCAAAAGCTGACCATCAAGAACTCAGGACTTCGGAGCATTCAGCCCAGAGCCTTTGCCAAGAACCCCCATTTGCGTTATATAAACCTGTCAAGTAACCGGCTCACCACACTCTCGTGGCAGCTCTTCCAGACGCTGAGTCTTCGGGAATTGCAGTTGGAGCAGAACTTTTTCAACTGCAGCTGTGACATCCGCTGGATGCAGCTCTGGCAGGAGCAGGGGGAGGCCAAGCTCAACAGCCAGAACCTCTACTGCATCAACGCTGATGGCTCCCAGCTTCCTCTCTTCCGCATGAACATCAGTCAGTGTGACCTTCCTGAGATCAGCGTGAGCCACGTCAACCTGACCGTACGAGAGGGTGACAATGCTGTTATCACTTGCAATGGCTCTGGATCACCCCTTCCTGATGTGGACTGGATAGTCACTGGGCTGCAGTCCATCAACACTCACCAGACCAATCTGAACTGGACCAATGTTCATGCCATCAACTTGACGCTGGTGAATGTGACGAGTGAGGACAATGGCTTCACCCTGACGTGCATTGCAGAGAACGTGGTGGGCATGAGCAATGCCAGTGTTGCCCTCACTGTCTACTATCCCCCACGTGTGGTGAGCCTGGAGGAGCCTGAGCTGCGCCTGGAGCACTGCATCGAGTTTGTGGTGCGTGGCAACCCCCCACCAACGCTGCACTGGCTGCACAATGGGCAGCCTCTGCGGGAGTCCAAGATCATCCATGTGGAATACTACCAAGAGGGAGAGATTTCCGAGGGCTGCCTGCTCTTCAACAAGCCCACCCACTACAACAATGGCAACTATACCCTCATTGCCAAAAACCCACTGGGCACAGCCAACCAGACCATCAATGGCCACTTCCTCAAGGAGCCCTTTCCAGAGAGCACGGATAACTTTATCTTGTTTGACGAAGTGAGTCCCACACCTCCTATCACTGTGACCCACAAACCAGAAGAAGACACTTTTGGGGTATCCATAGCAGTTGGACTTGCTGCTTTTGCCTGTGTCCTGTTGGTGGTTCTCTTCGTCATGATCAACAAATATGGTCGACGGTCCAAATTTGGAATGAAGGGTCCCGTGGCTGTCATCAGTGGTGAGGAGGACTCAGCCAGCCCACTGCACCACATCAACCACGGCATCACCACGCCCTCGTCACTGGATGCGGGGCCCGACACTGTGGTCATTGGCATGACTCGCATCCCTGTCATTGAGAACCCCCAGTACTTCCGTCAGGGACACAACTGCCACAAGCCGGACACGTATGTGCAGCACATTAAGAGGAGAGACATCGTGCTGAAGCGAGAACTGGGTGAGGGAGCCTTTGGAAAGGTCTTCCTGGCCGAGTGCTACAACCTCAGCCCGACCAAGGACAAGATGCTTGTGGCTGTGAAGGCCCTGAAGGATCCCACCCTGGCTGCCCGGAAGGATTTCCAGAGGGAGGCCGAGCTGCTCACCAACCTGCAGCATGAGCACATTGTCAAGTTCTATGGAGTGTGCGGCGATGGGGACCCCCTCATCATGGTCTTTGAATACATGAAGCATGGAGACCTGAATAAGTTCCTCAGGGCCCATGGGCCAAATGCAATGATCCTTGTGGATGGACAGCCACGCCAGGCCAAGGGTGAGCTGGGGCTCTCCCAAATGCTCCACATTGCCAGTCAGATCGCCTCGGGTATGGTGTACCTGGCCTCCCAGCACTTTGTGCACCGAGACCTGGCCACCAGGAACTGCCTGGTTGGAGCGAATCTGCTAGTGAAGATTGGGGACTTCGGCATGTCCAGAGATGTCTACAGCACGGATTATTACAGGGTGGGAGGACACACCATGCTCCCCATTCGCTGGATGCCTCCTGAAAGCATCATGTACCGGAAGTTCACTACAGAGAGTGATGTATGGAGCTTCGGGGTGATCCTCTGGGAGATCTTCACCTATGGAAAGCAGCCATGGTTCCAACTCTCAAACACGGAGGTCATTGAGTGCATTACCCAAGGTCGTGTTTTGGAGCGGCCCCGAGTCTGCCCCAAAGAGGTGTACGATGTCATGCTGGGGTGCTGGCAGAGGGAACCACAGCAGCGGTTGAACATCAAGGAGATCTACAAAATCCTCCATGCTTTGGGGAAGGCCACCCCAATCTACCTGGACATTCTTGGCTAGTGGTGGCTGGTGGTCATGAATTCATACTCTGTTGCCTCCTCTCTCCCTGCCTCACATCTCCCTTCCACCTCACAACTCCTTCCAGCCTTGACTGAAGCGAACATCTTCATATAAACT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31647, GC content failed 500, low tm 9310, high tm 15080, high any compl 2, high end compl 45, high repeat similarity 25, long poly-x seq 21, ok 6664
PRIMER_RIGHT_EXPLAIN=considered 31647, GC content failed 60, low tm 9661, high tm 15014, high any compl 3, high end compl 12, high repeat similarity 13, long poly-x seq 21, ok 6863
PRIMER_PAIR_EXPLAIN=considered 740, unacceptable product size 675, high end compl 4, ok 61
PRIMER_PAIR_PENALTY=0.1156
PRIMER_LEFT_PENALTY=0.041227
PRIMER_RIGHT_PENALTY=0.074396
PRIMER_LEFT_SEQUENCE=GTATCCATAGCAGTTGGACTTGC
PRIMER_RIGHT_SEQUENCE=CCCTGTAATAATCCGTGCTGTAG
PRIMER_LEFT=1448,23
PRIMER_RIGHT=2288,23
PRIMER_LEFT_TM=60.041
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=841
PRIMER_PAIR_PENALTY_1=0.1315
PRIMER_LEFT_1_PENALTY=0.088390
PRIMER_RIGHT_1_PENALTY=0.043090
PRIMER_LEFT_1_SEQUENCE=ACATCAGTCAGTGTGACCTTCCT
PRIMER_RIGHT_1_SEQUENCE=CAGTGACTATCCAGTCCACATCA
PRIMER_LEFT_1=762,23
PRIMER_RIGHT_1=893,23
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.043
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.1491
PRIMER_LEFT_2_PENALTY=0.074396
PRIMER_RIGHT_2_PENALTY=0.074695
PRIMER_LEFT_2_SEQUENCE=CTACAGCACGGATTATTACAGGG
PRIMER_RIGHT_2_SEQUENCE=AGCATGACATCGTACACCTCTTT
PRIMER_LEFT_2=2266,23
PRIMER_RIGHT_2=2523,23
PRIMER_LEFT_2_TM=59.926
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=10.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.1687
PRIMER_LEFT_3_PENALTY=0.094334
PRIMER_RIGHT_3_PENALTY=0.074396
PRIMER_LEFT_3_SEQUENCE=GGTATCCATAGCAGTTGGACTTG
PRIMER_RIGHT_3_SEQUENCE=CCCTGTAATAATCCGTGCTGTAG
PRIMER_LEFT_3=1447,23
PRIMER_RIGHT_3=2288,23
PRIMER_LEFT_3_TM=59.906
PRIMER_RIGHT_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MARINER1 Human mariner
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=842
PRIMER_PAIR_PENALTY_4=0.2257
PRIMER_LEFT_4_PENALTY=0.207373
PRIMER_RIGHT_4_PENALTY=0.018344
PRIMER_LEFT_4_SEQUENCE=CTGACCATCAAGAACTCAGGACT
PRIMER_RIGHT_4_SEQUENCE=GATAACAGCATTGTCACCCTCTC
PRIMER_LEFT_4=476,23
PRIMER_RIGHT_4=841,23
PRIMER_LEFT_4_TM=59.793
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=366
PRIMER_PAIR_PENALTY_5=0.2351
PRIMER_LEFT_5_PENALTY=0.041227
PRIMER_RIGHT_5_PENALTY=0.193896
PRIMER_LEFT_5_SEQUENCE=GTATCCATAGCAGTTGGACTTGC
PRIMER_RIGHT_5_SEQUENCE=ACCCTGTAATAATCCGTGCTGTA
PRIMER_LEFT_5=1448,23
PRIMER_RIGHT_5=2289,23
PRIMER_LEFT_5_TM=60.041
PRIMER_RIGHT_5_TM=59.806
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=842
PRIMER_PAIR_PENALTY_6=0.2491
PRIMER_LEFT_6_PENALTY=0.018344
PRIMER_RIGHT_6_PENALTY=0.230707
PRIMER_LEFT_6_SEQUENCE=GAGAGGGTGACAATGCTGTTATC
PRIMER_RIGHT_6_SEQUENCE=GGATACCCCAAAAGTGTCTTCTT
PRIMER_LEFT_6=819,23
PRIMER_RIGHT_6=1453,23
PRIMER_LEFT_6_TM=60.018
PRIMER_RIGHT_6_TM=59.769
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.9000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=635
PRIMER_PAIR_PENALTY_7=0.2505
PRIMER_LEFT_7_PENALTY=0.207373
PRIMER_RIGHT_7_PENALTY=0.043090
PRIMER_LEFT_7_SEQUENCE=CTGACCATCAAGAACTCAGGACT
PRIMER_RIGHT_7_SEQUENCE=CAGTGACTATCCAGTCCACATCA
PRIMER_LEFT_7=476,23
PRIMER_RIGHT_7=893,23
PRIMER_LEFT_7_TM=59.793
PRIMER_RIGHT_7_TM=60.043
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=418
PRIMER_PAIR_PENALTY_8=0.2566
PRIMER_LEFT_8_PENALTY=0.018344
PRIMER_RIGHT_8_PENALTY=0.238256
PRIMER_LEFT_8_SEQUENCE=GAGAGGGTGACAATGCTGTTATC
PRIMER_RIGHT_8_SEQUENCE=TTTTGGCAATGAGGGTATAGTTG
PRIMER_LEFT_8=819,23
PRIMER_RIGHT_8=1298,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=60.238
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_8_END_STABILITY=5.9000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=480
PRIMER_PAIR_PENALTY_9=0.2652
PRIMER_LEFT_9_PENALTY=0.207373
PRIMER_RIGHT_9_PENALTY=0.057845
PRIMER_LEFT_9_SEQUENCE=CTGACCATCAAGAACTCAGGACT
PRIMER_RIGHT_9_SEQUENCE=AGTGATAACAGCATTGTCACCCT
PRIMER_LEFT_9=476,23
PRIMER_RIGHT_9=844,23
PRIMER_LEFT_9_TM=59.793
PRIMER_RIGHT_9_TM=59.942
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=9.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=369
PRIMER_PAIR_PENALTY_10=0.2686
PRIMER_LEFT_10_PENALTY=0.193896
PRIMER_RIGHT_10_PENALTY=0.074695
PRIMER_LEFT_10_SEQUENCE=TACAGCACGGATTATTACAGGGT
PRIMER_RIGHT_10_SEQUENCE=AGCATGACATCGTACACCTCTTT
PRIMER_LEFT_10=2267,23
PRIMER_RIGHT_10=2523,23
PRIMER_LEFT_10_TM=59.806
PRIMER_RIGHT_10_TM=60.075
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_10_MISPRIMING_SCORE=10.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=257
PRIMER_PAIR_PENALTY_11=0.2695
PRIMER_LEFT_11_PENALTY=0.018344
PRIMER_RIGHT_11_PENALTY=0.251135
PRIMER_LEFT_11_SEQUENCE=GAGAGGGTGACAATGCTGTTATC
PRIMER_RIGHT_11_SEQUENCE=ATGAACATTGGTCCAGTTCAGAT
PRIMER_LEFT_11=819,23
PRIMER_RIGHT_11=946,23
PRIMER_LEFT_11_TM=60.018
PRIMER_RIGHT_11_TM=59.749
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, MER4B a consensus
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=128
PRIMER_PAIR_PENALTY_12=0.2738
PRIMER_LEFT_12_PENALTY=0.043090
PRIMER_RIGHT_12_PENALTY=0.230707
PRIMER_LEFT_12_SEQUENCE=TGATGTGGACTGGATAGTCACTG
PRIMER_RIGHT_12_SEQUENCE=GGATACCCCAAAAGTGTCTTCTT
PRIMER_LEFT_12=871,23
PRIMER_RIGHT_12=1453,23
PRIMER_LEFT_12_TM=60.043
PRIMER_RIGHT_12_TM=59.769
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=583
PRIMER_PAIR_PENALTY_13=0.2813
PRIMER_LEFT_13_PENALTY=0.043090
PRIMER_RIGHT_13_PENALTY=0.238256
PRIMER_LEFT_13_SEQUENCE=TGATGTGGACTGGATAGTCACTG
PRIMER_RIGHT_13_SEQUENCE=TTTTGGCAATGAGGGTATAGTTG
PRIMER_LEFT_13=871,23
PRIMER_RIGHT_13=1298,23
PRIMER_LEFT_13_TM=60.043
PRIMER_RIGHT_13_TM=60.238
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=428
PRIMER_PAIR_PENALTY_14=0.2833
PRIMER_LEFT_14_PENALTY=0.074396
PRIMER_RIGHT_14_PENALTY=0.208920
PRIMER_LEFT_14_SEQUENCE=CTACAGCACGGATTATTACAGGG
PRIMER_RIGHT_14_SEQUENCE=CTAGCCAAGAATGTCCAGGTAGA
PRIMER_LEFT_14=2266,23
PRIMER_RIGHT_14=2632,23
PRIMER_LEFT_14_TM=59.926
PRIMER_RIGHT_14_TM=59.791
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=5.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=367
PRIMER_PAIR_PENALTY_15=0.2882
PRIMER_LEFT_15_PENALTY=0.094334
PRIMER_RIGHT_15_PENALTY=0.193896
PRIMER_LEFT_15_SEQUENCE=GGTATCCATAGCAGTTGGACTTG
PRIMER_RIGHT_15_SEQUENCE=ACCCTGTAATAATCCGTGCTGTA
PRIMER_LEFT_15=1447,23
PRIMER_RIGHT_15=2289,23
PRIMER_LEFT_15_TM=59.906
PRIMER_RIGHT_15_TM=59.806
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=5.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=843
PRIMER_PAIR_PENALTY_16=0.2958
PRIMER_LEFT_16_PENALTY=0.207373
PRIMER_RIGHT_16_PENALTY=0.088390
PRIMER_LEFT_16_SEQUENCE=CTGACCATCAAGAACTCAGGACT
PRIMER_RIGHT_16_SEQUENCE=AGGAAGGTCACACTGACTGATGT
PRIMER_LEFT_16=476,23
PRIMER_RIGHT_16=784,23
PRIMER_LEFT_16_TM=59.793
PRIMER_RIGHT_16_TM=60.088
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.6000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=309
PRIMER_PAIR_PENALTY_17=0.3191
PRIMER_LEFT_17_PENALTY=0.088390
PRIMER_RIGHT_17_PENALTY=0.230707
PRIMER_LEFT_17_SEQUENCE=ACATCAGTCAGTGTGACCTTCCT
PRIMER_RIGHT_17_SEQUENCE=GGATACCCCAAAAGTGTCTTCTT
PRIMER_LEFT_17=762,23
PRIMER_RIGHT_17=1453,23
PRIMER_LEFT_17_TM=60.088
PRIMER_RIGHT_17_TM=59.769
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=692
PRIMER_PAIR_PENALTY_18=0.3266
PRIMER_LEFT_18_PENALTY=0.088390
PRIMER_RIGHT_18_PENALTY=0.238256
PRIMER_LEFT_18_SEQUENCE=ACATCAGTCAGTGTGACCTTCCT
PRIMER_RIGHT_18_SEQUENCE=TTTTGGCAATGAGGGTATAGTTG
PRIMER_LEFT_18=762,23
PRIMER_RIGHT_18=1298,23
PRIMER_LEFT_18_TM=60.088
PRIMER_RIGHT_18_TM=60.238
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=537
PRIMER_PAIR_PENALTY_19=0.3395
PRIMER_LEFT_19_PENALTY=0.088390
PRIMER_RIGHT_19_PENALTY=0.251135
PRIMER_LEFT_19_SEQUENCE=ACATCAGTCAGTGTGACCTTCCT
PRIMER_RIGHT_19_SEQUENCE=ATGAACATTGGTCCAGTTCAGAT
PRIMER_LEFT_19=762,23
PRIMER_RIGHT_19=946,23
PRIMER_LEFT_19_TM=60.088
PRIMER_RIGHT_19_TM=59.749
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=185
=