PRIMER_SEQUENCE_ID=NM_002609
SEQUENCE=GGCCCCTCAGCCCTGCTGCCCAGCACGAGCCTGTGCTCGCCCTGCCCAACGCAGACAGCCAGACCCAGGGCGGCCCCTCTGGCGGCTCTGCTCCTCCCGAAGGATGCTTGGGGAGTGAGGCGAAGCTGGGCGCTCCTCTCCCCTACAGCAGCCCCCTTCCTCCATCCCTCTGTTCTCCTGAGCCTTCAGGAGCCTGCACCAGTCCTGCCTGTCCTTCTACTCAGCTGTTACCCACTCTGGGACCAGCAGTCTTTCTGATAACTGGGAGAGGGCAGTAAGGAGGACTTCCTGGAGGGGGTGACTGTCCAGAGCCTGGAACTGTGCCCACACCAGAAGCCATCAGCAGCAAGGACACCATGCGGCTTCCGGGTGCGATGCCAGCTCTGGCCCTCAAAGGCGAGCTGCTGTTGCTGTCTCTCCTGTTACTTCTGGAACCACAGATCTCTCAGGGCCTGGTCGTCACACCCCCGGGGCCAGAGCTTGTCCTCAATGTCTCCAGCACCTTCGTTCTGACCTGCTCGGGTTCAGCTCCGGTGGTGTGGGAACGGATGTCCCAGGAGCCCCCACAGGAAATGGCCAAGGCCCAGGATGGCACCTTCTCCAGCGTGCTCACACTGACCAACCTCACTGGGCTAGACACGGGAGAATACTTTTGCACCCACAATGACTCCCGTGGACTGGAGACCGATGAGCGGAAACGGCTCTACATCTTTGTGCCAGATCCCACCGTGGGCTTCCTCCCTAATGATGCCGAGGAACTATTCATCTTTCTCACGGAAATAACTGAGATCACCATTCCATGCCGAGTAACAGACCCACAGCTGGTGGTGACACTGCACGAGAAGAAAGGGGACGTTGCACTGCCTGTCCCCTATGATCACCAACGTGGCTTTTCTGGTATCTTTGAGGACAGAAGCTACATCTGCAAAACCACCATTGGGGACAGGGAGGTGGATTCTGATGCCTACTATGTCTACAGACTCCAGGTGTCATCCATCAACGTCTCTGTGAACGCAGTGCAGACTGTGGTCCGCCAGGGTGAGAACATCACCCTCATGTGCATTGTGATCGGGAATGAGGTGGTCAACTTCGAGTGGACATACCCCCGCAAAGAAAGTGGGCGGCTGGTGGAGCCGGTGACTGACTTCCTCTTGGATATGCCTTACCACATCCGCTCCATCCTGCACATCCCCAGTGCCGAGTTAGAAGACTCGGGGACCTACACCTGCAATGTGACGGAGAGTGTGAATGACCATCAGGATGAAAAGGCCATCAACATCACCGTGGTTGAGAGCGGCTACGTGCGGCTCCTGGGAGAGGTGGGCACACTACAATTTGCTGAGCTGCATCGGAGCCGGACACTGCAGGTAGTGTTCGAGGCCTACCCACCGCCCACTGTCCTGTGGTTCAAAGACAACCGCACCCTGGGCGACTCCAGCGCTGGCGAAATCGCCCTGTCCACGCGCAACGTGTCGGAGACCCGGTATGTGTCAGAGCTGACACTGGTTCGCGTGAAGGTGGCAGAGGCTGGCCACTACACCATGCGGGCCTTCCATGAGGATGCTGAGGTCCAGCTCTCCTTCCAGCTACAGATCAATGTCCCTGTCCGAGTGCTGGAGCTAAGTGAGAGCCACCCTGACAGTGGGGAACAGACAGTCCGCTGTCGTGGCCGGGGCATGCCCCAGCCGAACATCATCTGGTCTGCCTGCAGAGACCTCAAAAGGTGTCCACGTGAGCTGCCGCCCACGCTGCTGGGGAACAGTTCCGAAGAGGAGAGCCAGCTGGAGACTAACGTGACGTACTGGGAGGAGGAGCAGGAGTTTGAGGTGGTGAGCACACTGCGTCTGCAGCACGTGGATCGGCCACTGTCGGTGCGCTGCACGCTGCGCAACGCTGTGGGCCAGGACACGCAGGAGGTCATCGTGGTGCCACACTCCTTGCCCTTTAAGGTGGTGGTGATCTCAGCCATCCTGGCCCTGGTGGTGCTCACCATCATCTCCCTTATCATCCTCATCATGCTTTGGCAGAAGAAGCCACGTTACGAGATCCGATGGAAGGTGATTGAGTCTGTGAGCTCTGACGGCCATGAGTACATCTACGTGGACCCCATGCAGCTGCCCTATGACTCCACGTGGGAGCTGCCGCGGGACCAGCTTGTGCTGGGACGCACCCTCGGCTCTGGGGCCTTTGGGCAGGTGGTGGAGGCCACGGCTCATGGCCTGAGCCATTCTCAGGCCACGATGAAAGTGGCCGTCAAGATGCTTAAATCCACAGCCCGCAGCAGTGAGAAGCAAGCCCTTATGTCGGAGCTGAAGATCATGAGTCACCTTGGGCCCCACCTGAACGTGGTCAACCTGTTGGGGGCCTGCACCAAAGGAGGACCCATCTATATCATCACTGAGTACTGCCGCTACGGAGACCTGGTGGACTACCTGCACCGCAACAAACACACCTTCCTGCAGCACCACTCCGACAAGCGCCGCCCGCCCAGCGCGGAGCTCTACAGCAATGCTCTGCCCGTTGGGCTCCCCCTGCCCAGCCATGTGTCCTTGACCGGGGAGAGCGACGGTGGCTACATGGACATGAGCAAGGACGAGTCGGTGGACTATGTGCCCATGCTGGACATGAAAGGAGACGTCAAATATGCAGACATCGAGTCCTCCAACTACATGGCCCCTTACGATAACTACGTTCCCTCTGCCCCTGAGAGGACCTGCCGAGCAACTTTGATCAACGAGTCTCCAGTGCTAAGCTACATGGACCTCGTGGGCTTCAGCTACCAGGTGGCCAATGGCATGGAGTTTCTGGCCTCCAAGAACTGCGTCCACAGAGACCTGGCGGCTAGGAACGTGCTCATCTGTGAAGGCAAGCTGGTCAAGATCTGTGACTTTGGCCTGGCTCGAGACATCATGCGGGACTCGAATTACATCTCCAAAGGCAGCACCTTTTTGCCTTTAAAGTGGATGGCTCCGGAGAGCATCTTCAACAGCCTCTACACCACCCTGAGCGACGTGTGGTCCTTCGGGATCCTGCTCTGGGAGATCTTCACCTTGGGTGGCACCCCTTACCCAGAGCTGCCCATGAACGAGCAGTTCTACAATGCCATCAAACGGGGTTACCGCATGGCCCAGCCTGCCCATGCCTCCGACGAGATCTATGAGATCATGCAGAAGTGCTGGGAAGAGAAGTTTGAGATTCGGCCCCCCTTCTCCCAGCTGGTGCTGCTTCTCGAGAGACTGTTGGGCGAAGGTTACAAAAAGAAGTACCAGCAGGTGGATGAGGAGTTTCTGAGGAGTGACCACCCAGCCATCCTTCGGTCCCAGGCCCGCTTGCCTGGGTTCCATGGCCTCCGATCTCCCCTGGACACCAGCTCCGTCCTCTATACTGCCGTGCAGCCCAATGAGGGTGACAACGACTATATCATCCCCCTGCCTGACCCCAAACCCGAGGTTGCTGACGAGGGCCCACTGGAGGGTTCCCCCAGCCTAGCCAGCTCCACCCTGAATGAAGTCAACACCTCCTCAACCATCTCCTGTGACAGCCCCCTGGAGCCCCAGGACGAACCAGAGCCAGAGCCCCAGCTTGAGCTCCAGGTGGAGCCGGAGCCAGAGCTGGAACAGTTGCCGGATTCGGGGTGCCCTGCGCCTCGGGCGGAAGCAGAGGATAGCTTCCTGTAGGGGGCTGGCCCCTACCCTGCCCTGCCTGAAGCTCCCCCCCTGCCAGCACCCAGCATCTCCTGGCCTGGCCTGACCGGGCTTCCTGTCAGCCAGGCTGCCCTTATCAGCTGTCCCCTTCTGGAAGCTTTCTGCTCCTGACGTGTTGTGCCCCAAACCCTGGGGCTGGCTTAGGAGGCAAGAAAACTGCAGGGGCCGTGACCAGCCCTCTGCCTCCAGGGAGGCCAACTGACTCTGAGCCAGGGTTCCCCCAGGGAACTCAGTTTTCCCATATGTAAGATGGGAAAGTTAGGCTTGATGACCCAGAATCTAGGATTCTCTCCCTGGCTGACAGGTGGGGAGACCGAATCCCTCCCTGGGAAGATTCTTGGAGTTACTGAGGTGGTAAATTAACTTTTTTCTGTTCAGCCAGCTACCCCTCAAGGAATCATAGCTCTCTCCTCGCACTTTTTATCCACCCAGGAGCTAGGGAAGAGACCCTAGCCTCCCTGGCTGCTGGCTGAGCTAGGGCCTAGCCTTGAGCAGTGTTGCCTCATCCAGAAGAAAGCCAGTCTCCTCCCTATGATGCCAGTCCCTGCGTTCCCTGGCCCGAGCTGGTCTGGGGCCATTAGGCAGCCTAATTAATGCTGGAGGCTGAGCCAAGTACAGGACACCCCCAGCCTGCAGCCCTTGCCCAGGGCACTTGGAGCACACGCAGCCATAGCAAGTGCCTGTGTCCCTGTCCTTCAGGCCCATCAGTCCTGGGGCTTTTTCTTTATCACCCTCAGTCTTAATCCATCCACCAGAGTCTAGAAGGCCAGACGGGCCCCGCATCTGTGATGAGAATGTAAATGTGCCAGTGTGGAGTGGCCACGTGTGTGTGCCAGTATATGGCCCTGGCTCTGCATTGGACCTGCTATGAGGCTTTGGAGGAATCCCTCACCCTCTCTGGGCCTCAGTTTCCCCTTCAAAAAATGAATAAGTCGGACTTATTAACTCTGAGTGCCTTGCCAGCACTAACATTCTAGAGTATTCCAGGTGGTTGCACATTTGTCCAGATGAAGCAAGGCCATATACCCTAAACTTCCATCCTGGGGGTCAGCTGGGCTCCTGGGAGATTCCAGATCACACATCACACTCTGGGGACTCAGGAACCATGCCCCTTCCCCAGGCCCCCAGCAAGTCTCAAGAACACAGCTGCACAGGCCTTGACTTAGAGTGACAGCCGGTGTCCTGGAAAGCCCCAAGCAGCTGCCCCAGGGACATGGGAAGACCACGGGACCTCTTTCACTACCCACGATGACCTCCGGGGGTATCCTGGGCAAAAGGGACAAAGAGGGCAAATGAGATCACCTCCTGCAGCCCACCACTCCAGCACCTGTGCCGAGGTCTGCGTCGAAGACAGAATGGACAGTGAGGACAGTTATGTCTTGTAAAAGACAAGAAGCTTCAGATGGTACCCCAAGAAGGATGTGAGAGGTGGCCGCTTGGAGTTTGCCCCTCACCCACCAGCTGCCCCATCCCTGAGGCAGCGCTCCATGGGGGTATGGTTTTGTCACTGCCCAGACCTAGCAGTGACATCTCATTGTCCCCAGCCCAGTGGGCATTGGAGGTGCCAGGGGAGTCAGGGTTGTAGCCAAGACGCCCCCGCACGGGGAGGGTTGGGAAGGGGGTGCAGGAAGCTCAACCCCTCTGGGCACCAACCCTGCATTGCAGGTTGGCACCTTACTTCCCTGGGATCCCCAGAGTTGGTCCAAGGAGGGAGAGTGGGTTCTCAATACGGTACCAAAGATATAATCACCTAGGTTTACAAATATTTTTAGGACTCACGTTAACTCACATTTATACAGCAGAAATGCTATTTTGTATGCTGTTAAGTTTTTCTATCTGTGTACTTTTTTTTAAGGGAAAGATTTTAATATTAAACCTGGTGCTTCTCACTCAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 65342, GC content failed 705, low tm 17645, high tm 33862, high any compl 18, high end compl 36, high repeat similarity 49, long poly-x seq 85, ok 12942
PRIMER_RIGHT_EXPLAIN=considered 65163, GC content failed 677, low tm 18211, high tm 33174, high end compl 10, high repeat similarity 13, long poly-x seq 108, ok 12970
PRIMER_PAIR_EXPLAIN=considered 952, unacceptable product size 889, high end compl 4, ok 59
PRIMER_PAIR_PENALTY=0.0615
PRIMER_LEFT_PENALTY=0.056582
PRIMER_RIGHT_PENALTY=0.004943
PRIMER_LEFT_SEQUENCE=ATAGCTCTCTCCTCGCACTTTTT
PRIMER_RIGHT_SEQUENCE=CTGGTGGATGGATTAAGACTGAG
PRIMER_LEFT=4103,23
PRIMER_RIGHT=4458,23
PRIMER_LEFT_TM=60.057
PRIMER_RIGHT_TM=60.005
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=356
PRIMER_PAIR_PENALTY_1=0.0631
PRIMER_LEFT_1_PENALTY=0.037129
PRIMER_RIGHT_1_PENALTY=0.026007
PRIMER_LEFT_1_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_1_SEQUENCE=TTGATCTGTAGCTGGAAGGAGAG
PRIMER_LEFT_1=630,23
PRIMER_RIGHT_1=1596,23
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=967
PRIMER_PAIR_PENALTY_2=0.0755
PRIMER_LEFT_2_PENALTY=0.026007
PRIMER_RIGHT_2_PENALTY=0.049498
PRIMER_LEFT_2_SEQUENCE=CTCTCCTTCCAGCTACAGATCAA
PRIMER_RIGHT_2_SEQUENCE=GATATAGATGGGTCCTCCTTTGG
PRIMER_LEFT_2=1574,23
PRIMER_RIGHT_2=2392,23
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.049
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_2_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=819
PRIMER_PAIR_PENALTY_3=0.0797
PRIMER_LEFT_3_PENALTY=0.043085
PRIMER_RIGHT_3_PENALTY=0.036623
PRIMER_LEFT_3_SEQUENCE=ATCAACGAGTCTCCAGTGCTAAG
PRIMER_RIGHT_3_SEQUENCE=GTGTAGAGGCTGTTGAAGATGCT
PRIMER_LEFT_3=2729,23
PRIMER_RIGHT_3=2997,23
PRIMER_LEFT_3_TM=59.957
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.0808
PRIMER_LEFT_4_PENALTY=0.026007
PRIMER_RIGHT_4_PENALTY=0.054756
PRIMER_LEFT_4_SEQUENCE=CTCTCCTTCCAGCTACAGATCAA
PRIMER_RIGHT_4_SEQUENCE=AGGATGATAAGGGAGATGATGGT
PRIMER_LEFT_4=1574,23
PRIMER_RIGHT_4=2010,23
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.055
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=437
PRIMER_PAIR_PENALTY_5=0.0861
PRIMER_LEFT_5_PENALTY=0.049498
PRIMER_RIGHT_5_PENALTY=0.036623
PRIMER_LEFT_5_SEQUENCE=CCAAAGGAGGACCCATCTATATC
PRIMER_RIGHT_5_SEQUENCE=GTGTAGAGGCTGTTGAAGATGCT
PRIMER_LEFT_5=2370,23
PRIMER_RIGHT_5=2997,23
PRIMER_LEFT_5_TM=60.049
PRIMER_RIGHT_5_TM=59.963
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.5000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=628
PRIMER_PAIR_PENALTY_6=0.0926
PRIMER_LEFT_6_PENALTY=0.049498
PRIMER_RIGHT_6_PENALTY=0.043085
PRIMER_LEFT_6_SEQUENCE=CCAAAGGAGGACCCATCTATATC
PRIMER_RIGHT_6_SEQUENCE=CTTAGCACTGGAGACTCGTTGAT
PRIMER_LEFT_6=2370,23
PRIMER_RIGHT_6=2751,23
PRIMER_LEFT_6_TM=60.049
PRIMER_RIGHT_6_TM=59.957
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.5000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=382
PRIMER_PAIR_PENALTY_7=0.0933
PRIMER_LEFT_7_PENALTY=0.004943
PRIMER_RIGHT_7_PENALTY=0.088390
PRIMER_LEFT_7_SEQUENCE=CTCAGTCTTAATCCATCCACCAG
PRIMER_RIGHT_7_SEQUENCE=ACTGTCCTCACTGTCCATTCTGT
PRIMER_LEFT_7=4436,23
PRIMER_RIGHT_7=5076,23
PRIMER_LEFT_7_TM=60.005
PRIMER_RIGHT_7_TM=60.088
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER4B a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=641
PRIMER_PAIR_PENALTY_8=0.1072
PRIMER_LEFT_8_PENALTY=0.102207
PRIMER_RIGHT_8_PENALTY=0.004943
PRIMER_LEFT_8_SEQUENCE=TAGGCTTGATGACCCAGAATCTA
PRIMER_RIGHT_8_SEQUENCE=CTGGTGGATGGATTAAGACTGAG
PRIMER_LEFT_8=3963,23
PRIMER_RIGHT_8=4458,23
PRIMER_LEFT_8_TM=60.102
PRIMER_RIGHT_8_TM=60.005
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=496
PRIMER_PAIR_PENALTY_9=0.1343
PRIMER_LEFT_9_PENALTY=0.108280
PRIMER_RIGHT_9_PENALTY=0.026007
PRIMER_LEFT_9_SEQUENCE=GCCGAGGAACTATTCATCTTTCT
PRIMER_RIGHT_9_SEQUENCE=TTGATCTGTAGCTGGAAGGAGAG
PRIMER_LEFT_9=749,23
PRIMER_RIGHT_9=1596,23
PRIMER_LEFT_9_TM=60.108
PRIMER_RIGHT_9_TM=60.026
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=848
PRIMER_PAIR_PENALTY_10=0.1450
PRIMER_LEFT_10_PENALTY=0.056582
PRIMER_RIGHT_10_PENALTY=0.088390
PRIMER_LEFT_10_SEQUENCE=ATAGCTCTCTCCTCGCACTTTTT
PRIMER_RIGHT_10_SEQUENCE=ACTGTCCTCACTGTCCATTCTGT
PRIMER_LEFT_10=4103,23
PRIMER_RIGHT_10=5076,23
PRIMER_LEFT_10_TM=60.057
PRIMER_RIGHT_10_TM=60.088
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=2.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=974
PRIMER_PAIR_PENALTY_11=0.1454
PRIMER_LEFT_11_PENALTY=0.037129
PRIMER_RIGHT_11_PENALTY=0.108280
PRIMER_LEFT_11_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_11_SEQUENCE=AGAAAGATGAATAGTTCCTCGGC
PRIMER_LEFT_11=630,23
PRIMER_RIGHT_11=771,23
PRIMER_LEFT_11_TM=60.037
PRIMER_RIGHT_11_TM=60.108
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=11.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=142
PRIMER_PAIR_PENALTY_12=0.1560
PRIMER_LEFT_12_PENALTY=0.130037
PRIMER_RIGHT_12_PENALTY=0.026007
PRIMER_LEFT_12_SEQUENCE=GGTGGATTCTGATGCCTACTATG
PRIMER_RIGHT_12_SEQUENCE=TTGATCTGTAGCTGGAAGGAGAG
PRIMER_LEFT_12=949,23
PRIMER_RIGHT_12=1596,23
PRIMER_LEFT_12_TM=59.870
PRIMER_RIGHT_12_TM=60.026
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MER5a a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=648
PRIMER_PAIR_PENALTY_13=0.1603
PRIMER_LEFT_13_PENALTY=0.037129
PRIMER_RIGHT_13_PENALTY=0.123139
PRIMER_LEFT_13_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_13_SEQUENCE=ATCCTGATGGTCATTCACACTCT
PRIMER_LEFT_13=630,23
PRIMER_RIGHT_13=1261,23
PRIMER_LEFT_13_TM=60.037
PRIMER_RIGHT_13_TM=59.877
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=632
PRIMER_PAIR_PENALTY_14=0.1612
PRIMER_LEFT_14_PENALTY=0.135150
PRIMER_RIGHT_14_PENALTY=0.026007
PRIMER_LEFT_14_SEQUENCE=GAGTTAGAAGACTCGGGGACCTA
PRIMER_RIGHT_14_SEQUENCE=TTGATCTGTAGCTGGAAGGAGAG
PRIMER_LEFT_14=1199,23
PRIMER_RIGHT_14=1596,23
PRIMER_LEFT_14_TM=60.135
PRIMER_RIGHT_14_TM=60.026
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=398
PRIMER_PAIR_PENALTY_15=0.1651
PRIMER_LEFT_15_PENALTY=0.160134
PRIMER_RIGHT_15_PENALTY=0.004943
PRIMER_LEFT_15_SEQUENCE=CTGAATGAAGTCAACACCTCCTC
PRIMER_RIGHT_15_SEQUENCE=CTGGTGGATGGATTAAGACTGAG
PRIMER_LEFT_15=3500,23
PRIMER_RIGHT_15=4458,23
PRIMER_LEFT_15_TM=60.160
PRIMER_RIGHT_15_TM=60.005
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=959
PRIMER_PAIR_PENALTY_16=0.1723
PRIMER_LEFT_16_PENALTY=0.037129
PRIMER_RIGHT_16_PENALTY=0.135150
PRIMER_LEFT_16_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_16_SEQUENCE=TAGGTCCCCGAGTCTTCTAACTC
PRIMER_LEFT_16=630,23
PRIMER_RIGHT_16=1221,23
PRIMER_LEFT_16_TM=60.037
PRIMER_RIGHT_16_TM=60.135
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=592
PRIMER_PAIR_PENALTY_17=0.1814
PRIMER_LEFT_17_PENALTY=0.037129
PRIMER_RIGHT_17_PENALTY=0.144318
PRIMER_LEFT_17_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_17_SEQUENCE=ACATTGATCTGTAGCTGGAAGGA
PRIMER_LEFT_17=630,23
PRIMER_RIGHT_17=1599,23
PRIMER_LEFT_17_TM=60.037
PRIMER_RIGHT_17_TM=60.144
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=970
PRIMER_PAIR_PENALTY_18=0.1814
PRIMER_LEFT_18_PENALTY=0.037129
PRIMER_RIGHT_18_PENALTY=0.144318
PRIMER_LEFT_18_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_18_SEQUENCE=GACATTGATCTGTAGCTGGAAGG
PRIMER_LEFT_18=630,23
PRIMER_RIGHT_18=1600,23
PRIMER_LEFT_18_TM=60.037
PRIMER_RIGHT_18_TM=60.144
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=971
PRIMER_PAIR_PENALTY_19=0.1814
PRIMER_LEFT_19_PENALTY=0.037129
PRIMER_RIGHT_19_PENALTY=0.144318
PRIMER_LEFT_19_SEQUENCE=GGCTAGACACGGGAGAATACTTT
PRIMER_RIGHT_19_SEQUENCE=GGACATTGATCTGTAGCTGGAAG
PRIMER_LEFT_19=630,23
PRIMER_RIGHT_19=1601,23
PRIMER_LEFT_19_TM=60.037
PRIMER_RIGHT_19_TM=60.144
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=972
=