PRIMER_SEQUENCE_ID=NM_002944
SEQUENCE=CAAGCTTTCAAGCATTCAAAGGTCTAAATGAAAAAGGCTAAGTATTATTTCAAAAGGCAAGTATATCCTAATATAGCAAAACAAACAAAGCAAAATCCATCAGCTACTCCTCCAATTGAAGTGATGAAGCCCAAATAATTCATATAGCAAAATGGAGAAAATTAGACCGGCCATCTAAAAATCTGCCATTGGTGAAGTGATGAAGAACATTTACTGTCTTATTCCGAAGCTTGTCAATTTTGCAACTCTTGGCTGCCTATGGATTTCTGTGGTGCAGTGTACAGTTTTAAATAGCTGCCTAAAGTCGTGTGTAACTAATCTGGGCCAGCAGCTTGACCTTGGCACACCACATAATCTGAGTGAACCGTGTATCCAAGGATGTCACTTTTGGAACTCTGTAGATCAGAAAAACTGTGCTTTAAAGTGTCGGGAGTCGTGTGAGGTTGGCTGTAGCAGCGCGGAAGGTGCATATGAAGAGGAAGTACTGGAAAATGCAGACCTACCAACTGCTCCCTTTGCTTCTTCCATTGGAAGCCACAATATGACATTACGATGGAAATCTGCAAACTTCTCTGGAGTAAAATACATCATTCAGTGGAAATATGCACAACTTCTGGGAAGCTGGACTTATACTAAGACTGTGTCCAGACCGTCCTATGTGGTCAAGCCCCTGCACCCCTTCACTGAGTACATTTTCCGAGTGGTTTGGATCTTCACAGCGCAGCTGCAGCTCTACTCCCCTCCAAGTCCCAGTTACAGGACTCATCCTCATGGAGTTCCTGAAACTGCACCTTTGATTAGGAATATTGAGAGCTCAAGTCCCGACACTGTGGAAGTCAGCTGGGATCCACCTCAATTCCCAGGTGGACCTATTTTGGGTTATAACTTAAGGCTGATCAGCAAAAATCAAAAATTAGATGCAGGGACACAGAGAACCAGTTTCCAGTTTTACTCCACTTTACCAAATACTATCTACAGGTTTTCTATTGCAGCAGTAAATGAAGTTGGTGAGGGTCCAGAAGCAGAATCTAGTATTACCACTTCATCTTCAGCAGTTCAACAAGAGGAACAGTGGCTCTTTTTATCCAGAAAAACTTCTCTAAGAAAGAGATCTTTAAAACATTTAGTAGATGAAGCACATTGCCTTCGGTTGGATGCTATATACCATAATATTACAGGAATATCTGTTGATGTCCACCAGCAAATTGTTTATTTCTCTGAAGGAACTCTCATATGGGCGAAGAAGGCTGCCAACATGTCTGATGTATCTGACCTGAGAATTTTTTACAGAGGTTCAGGATTAATTTCTTCTATCTCCATAGATTGGCTTTATCAAAGAATGTATTTCATCATGGATGAACTGGTATGTGTCTGTGATTTAGAGAACTGCTCAAACATCGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAATGGGTATGTCTTTTACCTCCTGAGAGATGGCATTTATAGAGCAGACCTTCCTGTACCATCTGGCCGGTGTGCAGAAGCTGTGCGTATTGTGGAGAGTTGCACGTTAAAGGACTTTGCAATCAAGCCACAAGCCAAGCGAATCATTTACTTCAATGACACTGCCCAAGTCTTCATGTCAACATTTCTGGATGGCTCTGCTTCCCATCTCATCCTACCTCGCATCCCCTTTGCTGATGTGAAAAGTTTTGCTTGTGAAAACAATGACTTTCTTGTCACAGATGGCAAGGTCATTTTCCAACAGGATGCTTTGTCTTTTAATGAATTCATCGTGGGATGTGACCTGAGTCACATAGAAGAATTTGGGTTTGGTAACTTGGTCATCTTTGGCTCATCCTCCCAGCTGCACCCTCTGCCAGGCCGCCCGCAGGAGCTTTCGGTGCTGTTTGGCTCTCACCAGGCTCTTGTTCAATGGAAGCCTCCTGCCCTTGCCATAGGAGCCAATGTCATCCTGATCAGTGATATTATTGAACTCTTTGAATTAGGCCCTTCTGCCTGGCAGAACTGGACCTATGAGGTGAAAGTATCCACCCAAGACCCTCCTGAAGTCACTCATATTTTCTTGAACATAAGTGGAACCATGCTGAATGTACCTGAGCTGCAGAGTGCTATGAAATACAAGGTTTCTGTGAGAGCAAGTTCTCCAAAGAGGCCAGGCCCCTGGTCAGAGCCCTCAGTGGGTACTACCCTGGTGCCAGCTAGTGAACCACCATTTATCATGGCTGTGAAAGAAGATGGGCTTTGGAGTAAACCATTAAATAGCTTTGGCCCAGGAGAGTTCTTATCCTCTGATATAGGAAATGTGTCAGACATGGATTGGTATAACAACAGCCTCTACTACAGTGACACGAAAGGCGACGTTTTTGTGTGGCTGCTGAATGGGACGGATATCTCAGAGAATTATCACCTACCCAGCATTGCAGGAGCAGGGGCTTTAGCTTTTGAGTGGCTGGGTCACTTTCTCTACTGGGCTGGAAAGACATATGTGATACAAAGGCAGTCTGTGTTGACGGGACACACAGACATTGTTACCCACGTGAAGCTATTGGTGAATGACATGGTGGTGGATTCAGTTGGTGGATATCTCTACTGGACCACACTCTATTCAGTGGAAAGCACCAGACTAAATGGGGAAAGTTCCCTTGTACTACAGACACAGCCTTGGTTTTCTGGGAAAAAGGTAATTGCTCTAACTTTAGACCTCAGTGATGGGCTCCTGTATTGGTTGGTTCAAGACAGTCAATGTATTCACCTGTACACAGCTGTTCTTCGGGGACAGAGCACTGGGGATACCACCATCACAGAATTTGCAGCCTGGAGTACTTCTGAAATTTCCCAGAATGCACTGATGTACTATAGTGGTCGGCTGTTCTGGATCAATGGCTTTAGGATTATCACAACTCAAGAAATAGGTCAGAAAACCAGTGTCTCTGTTTTGGAACCAGCCAGATTTAATCAGTTCACAATTATTCAGACATCCCTTAAGCCCCTGCCAGGGAACTTTTCCTTTACCCCTAAGGTTATTCCAGATTCTGTTCAAGAGTCTTCATTTAGGATTGAAGGAAATGCTTCAAGTTTTCAAATCCTGTGGAATGGTCCCCCTGCGGTAGACTGGGGTGTAGTTTTCTACAGTGTAGAATTTAGTGCTCATTCTAAGTTCTTGGCTAGTGAACAACACTCTTTACCTGTATTTACTGTGGAAGGACTGGAACCTTATGCCTTATTTAATCTTTCTGTCACTCCTTATACCTACTGGGGAAAGGGCCCCAAAACATCTCTGTCACTTCGAGCACCTGAAACAGTTCCATCAGCACCAGAGAACCCCAGAATATTTATATTACCAAGTGGAAAATGCTGCAACAAGAATGAAGTTGTGGTGGAATTTAGGTGGAACAAACCTAAGCATGAAAATGGGGTGTTAACAAAATTTGAAATTTTCTACAATATATCCAATCAAAGTATTACAAACAAAACATGTGAAGACTGGATTGCTGTCAATGTCACTCCCTCAGTGATGTCTTTTCAACTTGAAGGCATGAGTCCCAGATGCTTTATTGCCTTCCAGGTTAGGGCCTTTACATCTAAGGGGCCAGGACCATATGCTGACGTTGTAAAGTCTACAACATCAGAAATCAACCCATTTCCTCACCTCATAACTCTTCTTGGTAACAAGATAGTTTTTTTAGATATGGATCAAAATCAAGTTGTGTGGACGTTTTCAGCAGAAAGAGTTATCAGTGCCGTTTGCTACACAGCTGATAATGAGATGGGATATTATGCTGAAGGGGACTCACTCTTTCTTCTGCACTTGCACAATCGCTCTAGCTCTGAGCTTTTCCAAGATTCACTGGTTTTTGATATCACAGTTATTACAATTGACTGGATTTCAAGGCACCTCTACTTTGCACTGAAAGAATCACAAAATGGAATGCAAGTATTTGATGTTGATCTTGAACACAAGGTGAAATATCCCAGAGAGGTGAAGATTCACAATAGGAATTCAACAATAATTTCTTTTTCTGTATATCCTCTTTTAAGTCGCTTGTATTGGACAGAAGTTTCCAATTTTGGCTACCAGATGTTCTACTACAGTATTATCAGTCACACCTTGCACCGAATTCTGCAACCCACAGCTACAAACCAACAAAACAAAAGGAATCAATGTTCTTGTAATGTGACTGAATTTGAGTTAAGTGGAGCAATGGCTATTGATACCTCTAACCTAGAGAAACCATTGATATACTTTGCCAAAGCACAAGAGATCTGGGCAATGGATCTGGAAGGCTGTCAGTGTTGGAGAGTTATCACAGTACCTGCTATGCTCGCAGGAAAAACCCTTGTTAGCTTAACTGTGGATGGAGATCTTATATACTGGATCATCACAGCAAAGGACAGCACACAGATTTATCAGGCAAAGAAAGGAAATGGGGCCATCGTTTCCCAGGTGAAGGCCCTAAGGAGTAGGCATATCTTGGCTTACAGTTCAGTTATGCAGCCTTTTCCAGATAAAGCGTTTCTGTCTCTAGCTTCAGACACTGTGGAACCAACTATACTTAATGCCACTAACACTAGCCTCACAATCAGATTACCTCTGGCCAAGACAAACCTCACATGGTATGGCATCACCAGCCCTACTCCAACATACCTGGTTTATTATGCAGAAGTTAATGACAGGAAAAACAGCTCTGACTTGAAATATAGAATTCTGGAATTTCAGGACAGTATAGCTCTTATTGAAGATTTACAACCATTTTCAACATACATGATACAGATAGCTGTAAAAAATTATTATTCAGATCCTTTGGAACATTTACCACCAGGAAAAGAGATTTGGGGAAAAACTAAAAATGGAGTACCAGAGGCAGTGCAGCTCATTAATACAACTGTGCGGTCAGACACCAGCCTCATTATATCTTGGAGAGAATCTCACAAGCCAAATGGACCTAAAGAATCAGTCCGTTATCAGTTGGCAATCTCACACCTGGCCCTAATTCCTGAAACTCCTCTAAGACAAAGTGAATTTCCAAATGGAAGGCTCACTCTCCTTGTTACTAGACTGTCTGGTGGAAATATTTATGTGTTAAAGGTTCTTGCCTGCCACTCTGAGGAAATGTGGTGTACAGAGAGTCATCCTGTCACTGTGGAAATGTTTAACACACCAGAGAAACCTTATTCCTTGGTTCCAGAGAACACTAGTTTGCAATTTAATTGGAAGGCTCCATTGAATGTTAACCTCATCAGATTTTGGGTTGAGCTACAGAAGTGGAAATACAATGAGTTTTACCATGTTAAAACTTCATGCAGCCAAGGTCCTGCTTATGTCTGTAATATCACAAATCTACAACCTTATACTTCATATAATGTCAGAGTAGTGGTGGTTTATAAGACGGGAGAAAATAGCACCTCACTTCCAGAAAGCTTTAAGACAAAAGCTGGAGTCCCAAATAAACCAGGCATTCCCAAATTACTAGAAGGGAGTAAAAATTCAATACAGTGGGAGAAAGCTGAAGATAATGGATGTAGAATTACATACTATATCCTTGAGATAAGAAAGAGCACTTCAAATAATTTACAGAACCAGAATTTAAGGTGGAAGATGACATTTAATGGATCCTGCAGTAGTGTTTGCACATGGAAGTCCAAAAACCTGAAAGGAATATTTCAGTTCAGAGTAGTAGCTGCAAATAATCTAGGGTTTGGTGAATATAGTGGAATCAGTGAGAATATTATATTAGTTGGAGATGATTTTTGGATACCAGAAACAAGTTTCATACTTACTATTATAGTTGGAATATTTCTGGTTGTTACAATCCCACTGACCTTTGTCTGGCATAGAAGATTAAAGAATCAAAAAAGTGCCAAGGAAGGGGTGACAGTGCTTATAAACGAAGACAAAGAGTTGGCTGAGCTGCGAGGTCTGGCAGCCGGAGTAGGCCTGGCTAATGCCTGCTATGCAATACATACTCTTCCAACCCAAGAGGAGATTGAAAATCTTCCTGCCTTCCCTCGGGAAAAACTGACTCTGCGTCTCTTGCTGGGAAGTGGAGCCTTTGGAGAAGTGTATGAAGGAACAGCAGTGGACATCTTAGGAGTTGGAAGTGGAGAAATCAAAGTAGCAGTGAAGACTTTGAAGAAGGGTTCCACAGACCAGGAGAAGATTGAATTCCTGAAGGAGGCACATCTGATGAGCAAATTTAATCATCCCAACATTCTGAAGCAGCTTGGAGTTTGTCTGCTGAATGAACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTTATTTGCGTAAAGCCCGGATGGCAACGTTTTATGGTCCTTTACTCACCTTGGTTGACCTTGTAGACCTGTGTGTAGATATTTCAAAAGGCTGTGTCTACTTGGAACGGATGCATTTCATTCACAGGGATCTGGCAGCTAGAAATTGCCTTGTTTCCGTGAAAGACTATACCAGTCCACGGATAGTGAAGATTGGAGACTTTGGACTCGCCAGAGACATCTATAAAAATGATTACTATAGAAAGAGAGGGGAAGGCCTGCTCCCAGTTCGGTGGATGGCTCCAGAAAGTTTGATGGATGGAATCTTCACTACTCAATCTGATGTATGGTCTTTTGGAATTCTGATTTGGGAGATTTTAACTCTTGGTCATCAGCCTTATCCAGCTCATTCCAACCTTGATGTGTTAAACTATGTGCAAACAGGAGGGAGACTGGAGCCACCAAGAAATTGTCCTGATGATCTGTGGAATTTAATGACCCAGTGCTGGGCTCAAGAACCCGACCAAAGACCTACTTTTCATAGAATTCAGGACCAACTTCAGTTATTCAGAAATTTTTTCTTAAATAGCATTTATAAGTCCAGAGATGAAGCAAACAACAGTGGAGTCATAAATGAAAGCTTTGAAGGTGAAGATGGCGATGTGATTTGTTTGAATTCAGATGACATTATGCCAGTTGCTTTAATGGAAACGAAGAACCGAGAAGGGTTAAACTATATGGTACTTGCTACAGAATGTGGCCAAGGTGAAGAAAAGTCTGAGGGTCCTCTAGGCTCCCAGGAATCTGAATCTTGTGGTCTGAGGAAAGAAGAGAAGGAACCACATGCAGACAAAGATTTCTGCCAAGAAAAACAAGTGGCTTACTGCCCTTCTGGCAAGCCTGAAGGCCTGAACTATGCCTGTCTCACTCACAGTGGATATGGAGATGGGTCTGATTAATAGCGTTGTTTGGGAAATAGAGAGTTGAGATAAACACTCTCATTCAGTAGTTACTGAAAGAAAACTCTGCTAGAATGATAAATGTCATGGTGGTCTATAACTCCAAATAAACAATGCAACGTTCC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 86451, GC content failed 1233, low tm 48740, high tm 16081, high any compl 6, high end compl 28, high repeat similarity 38, long poly-x seq 115, ok 20210
PRIMER_RIGHT_EXPLAIN=considered 86407, GC content failed 1166, low tm 48780, high tm 16080, high end compl 12, high repeat similarity 13, long poly-x seq 119, ok 20237
PRIMER_PAIR_EXPLAIN=considered 1356, unacceptable product size 1268, high any compl 1, high end compl 14, ok 73
PRIMER_PAIR_PENALTY=0.0184
PRIMER_LEFT_PENALTY=0.009608
PRIMER_RIGHT_PENALTY=0.008837
PRIMER_LEFT_SEQUENCE=ACTCCTCCAATTGAAGTGATGAA
PRIMER_RIGHT_SEQUENCE=ACTCCATGAGGATGAGTCCTGTA
PRIMER_LEFT=105,23
PRIMER_RIGHT=776,23
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=672
PRIMER_PAIR_PENALTY_1=0.0192
PRIMER_LEFT_1_PENALTY=0.004943
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CCTCACAATCAGATTACCTCTGG
PRIMER_RIGHT_1_SEQUENCE=GTAGCTCAACCCAAAATCTGATG
PRIMER_LEFT_1=4587,23
PRIMER_RIGHT_1=5272,23
PRIMER_LEFT_1_TM=60.005
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=686
PRIMER_PAIR_PENALTY_2=0.0192
PRIMER_LEFT_2_PENALTY=0.010384
PRIMER_RIGHT_2_PENALTY=0.008837
PRIMER_LEFT_2_SEQUENCE=CTAAAAATCTGCCATTGGTGAAG
PRIMER_RIGHT_2_SEQUENCE=ACTCCATGAGGATGAGTCCTGTA
PRIMER_LEFT_2=174,23
PRIMER_RIGHT_2=776,23
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=60.009
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=10.00, MER4C a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=603
PRIMER_PAIR_PENALTY_3=0.0206
PRIMER_LEFT_3_PENALTY=0.017590
PRIMER_RIGHT_3_PENALTY=0.003045
PRIMER_LEFT_3_SEQUENCE=GGATTTCAAGGCACCTCTACTTT
PRIMER_RIGHT_3_SEQUENCE=TATCAATAGCCATTGCTCCACTT
PRIMER_LEFT_3=3896,23
PRIMER_RIGHT_3=4228,23
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MER21 a consensus
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=333
PRIMER_PAIR_PENALTY_4=0.0206
PRIMER_LEFT_4_PENALTY=0.017590
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=GGATTTCAAGGCACCTCTACTTT
PRIMER_RIGHT_4_SEQUENCE=ATCAATAGCCATTGCTCCACTTA
PRIMER_LEFT_4=3896,23
PRIMER_RIGHT_4=4227,23
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MER21 a consensus
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=332
PRIMER_PAIR_PENALTY_5=0.0272
PRIMER_LEFT_5_PENALTY=0.009608
PRIMER_RIGHT_5_PENALTY=0.017590
PRIMER_LEFT_5_SEQUENCE=ACTCCTCCAATTGAAGTGATGAA
PRIMER_RIGHT_5_SEQUENCE=GGATAAAAAGAGCCACTGTTCCT
PRIMER_LEFT_5=105,23
PRIMER_RIGHT_5=1086,23
PRIMER_LEFT_5_TM=59.990
PRIMER_RIGHT_5_TM=60.018
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=982
PRIMER_PAIR_PENALTY_6=0.0280
PRIMER_LEFT_6_PENALTY=0.010384
PRIMER_RIGHT_6_PENALTY=0.017590
PRIMER_LEFT_6_SEQUENCE=CTAAAAATCTGCCATTGGTGAAG
PRIMER_RIGHT_6_SEQUENCE=GGATAAAAAGAGCCACTGTTCCT
PRIMER_LEFT_6=174,23
PRIMER_RIGHT_6=1086,23
PRIMER_LEFT_6_TM=60.010
PRIMER_RIGHT_6_TM=60.018
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, MER4C a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=913
PRIMER_PAIR_PENALTY_7=0.0316
PRIMER_LEFT_7_PENALTY=0.022788
PRIMER_RIGHT_7_PENALTY=0.008837
PRIMER_LEFT_7_SEQUENCE=CATTGGTGAAGTGATGAAGAACA
PRIMER_RIGHT_7_SEQUENCE=ACTCCATGAGGATGAGTCCTGTA
PRIMER_LEFT_7=186,23
PRIMER_RIGHT_7=776,23
PRIMER_LEFT_7_TM=60.023
PRIMER_RIGHT_7_TM=60.009
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=591
PRIMER_PAIR_PENALTY_8=0.0323
PRIMER_LEFT_8_PENALTY=0.017590
PRIMER_RIGHT_8_PENALTY=0.014712
PRIMER_LEFT_8_SEQUENCE=AGGAACAGTGGCTCTTTTTATCC
PRIMER_RIGHT_8_SEQUENCE=TCAGATACATCAGACATGTTGGC
PRIMER_LEFT_8=1064,23
PRIMER_RIGHT_8=1271,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=60.015
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_8_END_STABILITY=7.1000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=208
PRIMER_PAIR_PENALTY_9=0.0334
PRIMER_LEFT_9_PENALTY=0.004943
PRIMER_RIGHT_9_PENALTY=0.028502
PRIMER_LEFT_9_SEQUENCE=CCTCACAATCAGATTACCTCTGG
PRIMER_RIGHT_9_SEQUENCE=CAAGTCAGAGCTGTTTTTCCTGT
PRIMER_LEFT_9=4587,23
PRIMER_RIGHT_9=4707,23
PRIMER_LEFT_9_TM=60.005
PRIMER_RIGHT_9_TM=59.971
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MER21 a consensus
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=121
PRIMER_PAIR_PENALTY_10=0.0356
PRIMER_LEFT_10_PENALTY=0.009608
PRIMER_RIGHT_10_PENALTY=0.026007
PRIMER_LEFT_10_SEQUENCE=ACTCCTCCAATTGAAGTGATGAA
PRIMER_RIGHT_10_SEQUENCE=GAGTTCCAAAAGTGACATCCTTG
PRIMER_LEFT_10=105,23
PRIMER_RIGHT_10=395,23
PRIMER_LEFT_10_TM=59.990
PRIMER_RIGHT_10_TM=60.026
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=291
PRIMER_PAIR_PENALTY_11=0.0364
PRIMER_LEFT_11_PENALTY=0.010384
PRIMER_RIGHT_11_PENALTY=0.026007
PRIMER_LEFT_11_SEQUENCE=CTAAAAATCTGCCATTGGTGAAG
PRIMER_RIGHT_11_SEQUENCE=GAGTTCCAAAAGTGACATCCTTG
PRIMER_LEFT_11=174,23
PRIMER_RIGHT_11=395,23
PRIMER_LEFT_11_TM=60.010
PRIMER_RIGHT_11_TM=60.026
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=10.00, MER4C a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=222
PRIMER_PAIR_PENALTY_12=0.0427
PRIMER_LEFT_12_PENALTY=0.028502
PRIMER_RIGHT_12_PENALTY=0.014220
PRIMER_LEFT_12_SEQUENCE=ACAGGAAAAACAGCTCTGACTTG
PRIMER_RIGHT_12_SEQUENCE=GTAGCTCAACCCAAAATCTGATG
PRIMER_LEFT_12=4685,23
PRIMER_RIGHT_12=5272,23
PRIMER_LEFT_12_TM=59.971
PRIMER_RIGHT_12_TM=60.014
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=588
PRIMER_PAIR_PENALTY_13=0.0457
PRIMER_LEFT_13_PENALTY=0.017590
PRIMER_RIGHT_13_PENALTY=0.028128
PRIMER_LEFT_13_SEQUENCE=AGAAGATGGGCTTTGGAGTAAAC
PRIMER_RIGHT_13_SEQUENCE=CATATGTCTTTCCAGCCCAGTAG
PRIMER_LEFT_13=2244,23
PRIMER_RIGHT_13=2500,23
PRIMER_LEFT_13_TM=60.018
PRIMER_RIGHT_13_TM=60.028
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.0000
PRIMER_RIGHT_13_END_STABILITY=5.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=257
PRIMER_PAIR_PENALTY_14=0.0461
PRIMER_LEFT_14_PENALTY=0.017590
PRIMER_RIGHT_14_PENALTY=0.028502
PRIMER_LEFT_14_SEQUENCE=GGATTTCAAGGCACCTCTACTTT
PRIMER_RIGHT_14_SEQUENCE=CAAGTCAGAGCTGTTTTTCCTGT
PRIMER_LEFT_14=3896,23
PRIMER_RIGHT_14=4707,23
PRIMER_LEFT_14_TM=60.018
PRIMER_RIGHT_14_TM=59.971
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=812
PRIMER_PAIR_PENALTY_15=0.0461
PRIMER_LEFT_15_PENALTY=0.028502
PRIMER_RIGHT_15_PENALTY=0.017590
PRIMER_LEFT_15_SEQUENCE=CAAGGTTTCTGTGAGAGCAAGTT
PRIMER_RIGHT_15_SEQUENCE=GTTTACTCCAAAGCCCATCTTCT
PRIMER_LEFT_15=2133,23
PRIMER_RIGHT_15=2266,23
PRIMER_LEFT_15_TM=59.971
PRIMER_RIGHT_15_TM=60.018
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=134
PRIMER_PAIR_PENALTY_16=0.0488
PRIMER_LEFT_16_PENALTY=0.022788
PRIMER_RIGHT_16_PENALTY=0.026007
PRIMER_LEFT_16_SEQUENCE=CATTGGTGAAGTGATGAAGAACA
PRIMER_RIGHT_16_SEQUENCE=GAGTTCCAAAAGTGACATCCTTG
PRIMER_LEFT_16=186,23
PRIMER_RIGHT_16=395,23
PRIMER_LEFT_16_TM=60.023
PRIMER_RIGHT_16_TM=60.026
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=210
PRIMER_PAIR_PENALTY_17=0.0538
PRIMER_LEFT_17_PENALTY=0.014220
PRIMER_RIGHT_17_PENALTY=0.039590
PRIMER_LEFT_17_SEQUENCE=CATCAGATTTTGGGTTGAGCTAC
PRIMER_RIGHT_17_SEQUENCE=AGGAATTCAATCTTCTCCTGGTC
PRIMER_LEFT_17=5250,23
PRIMER_RIGHT_17=6182,23
PRIMER_LEFT_17_TM=60.014
PRIMER_RIGHT_17_TM=59.960
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=933
PRIMER_PAIR_PENALTY_18=0.0540
PRIMER_LEFT_18_PENALTY=0.017590
PRIMER_RIGHT_18_PENALTY=0.036444
PRIMER_LEFT_18_SEQUENCE=GGATTTCAAGGCACCTCTACTTT
PRIMER_RIGHT_18_SEQUENCE=CAATAGCCATTGCTCCACTTAAC
PRIMER_LEFT_18=3896,23
PRIMER_RIGHT_18=4225,23
PRIMER_LEFT_18_TM=60.018
PRIMER_RIGHT_18_TM=60.036
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=330
PRIMER_PAIR_PENALTY_19=0.0558
PRIMER_LEFT_19_PENALTY=0.014220
PRIMER_RIGHT_19_PENALTY=0.041548
PRIMER_LEFT_19_SEQUENCE=CATCAGATTTTGGGTTGAGCTAC
PRIMER_RIGHT_19_SEQUENCE=AAGATTTTCAATCTCCTCTTGGG
PRIMER_LEFT_19=5250,23
PRIMER_RIGHT_19=6009,23
PRIMER_LEFT_19_TM=60.014
PRIMER_RIGHT_19_TM=59.958
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=10.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=760
=