PRIMER_SEQUENCE_ID=NM_003328
SEQUENCE=GATTTCAGTTGAAAGATGTGTTTTTGTGAGTAGAGCACCGCAGAAGAACTGAAGACTGTTGTGTGCTCCCCGCAGAAGGGGCTACCATGATCCTTTCCTCCTATAACACCATCCAGTCGGTTTTCTGTTGCTGCTGTTGCTGTTCAGTGCAGAAGCGACAAATGAGAACACAGATAAGCCTGAGCACAGATGAAGAGCTTCCAGAAAAATACACCCAGCATCGCAGGCCGTGGCTCAGCCAATTGTCAAATAAGAAGCAATCCAACACGGGCCGTGTGCAGCCGTCAAAACGAAAGCCACTGCCTCCCCTCCCACCCTCTGAGGTTGCTGAAGAGAAGATCCAAGTCAAGGCACTTTATGATTTTCTGCCCAGAGAACCCTGTAATTTAGCCTTAAGGAGAGCAGAAGAATACCTGATACTGGAGAAATACAATCCTCACTGGTGGAAGGCAAGAGACCGTTTGGGGAATGAAGGCTTAATCCCAAGCAACTATGTGACTGAAAACAAAATAACTAATTTAGAAATATATGAGTGGTACCATAGAAACATTACCAGAAATCAGGCAGAACATCTATTGAGACAAGAGTCTAAAGAAGGTGCATTTATTGTCAGAGATTCAAGACATTTAGGATCCTACACAATTTCCGTATTTATGGGAGCTAGAAGAAGTACGGAGGCTGCCATAAAACATTATCAGATAAAAAAGAATGACTCAGGACAGTGGTATGTGGCTGAAAGACACGCCTTTCAATCAATCCCTGAGTTAATCTGGTATCACCAGCACAATGCAGCCGGTCTCATGACTCGTCTCCGATATCCAGTTGGGCTGATGGGCAGTTGTTTACCAGCCACAGCTGGGTTTAGCTACGAAAAGTGGGAGATAGATCCATCTGAGTTGGCTTTTATAAAGGAGATTGGAAGCGGTCAGTTTGGAGTGGTCCATTTAGGTGAATGGCGGTCACATATCCAGGTAGCTATCAAGGCCATCAATGAAGGCTCCATGTCTGAAGAGGATTTCATTGAAGAGGCCAAAGTGATGATGAAATTATCTCATTCAAAGCTAGTGCAACTTTATGGAGTCTGTATACAGCGGAAGCCCCTTTACATTGTGACAGAGTTCATGGAAAATGGCTGCCTGCTTAACTATCTCAGGGAGAATAAAGGAAAGCTTAGGAAGGAAATGCTACTGAGTGTATGCCAGGATATATGTGAAGGAATGGAATATCTGGAGAGGAATGGCTATATTCATAGGGATTTGGCGGCAAGGAATTGTTTGGTCAGTTCAACATGCATAGTAAAAATTTCAGACTTTGGAATGACAAGGTACGTTTTGGATGATGAGTATGTCAGTTCTTTTGGAGCCAAGTTCCCAATCAAGTGGTCCCCTCCTGAAGTTTTTCTTTTCAATAAGTACAGCAGTAAATCTGATGTCTGGTCATTTGGAGTTTTAATGTGGGAAGTTTTTACAGAAGGAAAAATGCCTTTTGAAAATAAGTCAAATTTGCAAGTCGTGGAAGCTATTTCTGAAGGCTTCAGGCTATATCGCCCTCACCTGGCACCAATGTCCATATATGAAGTCATGTACAGCTGCTGGCATGAGAAACCTGAAGGCCGCCCTACATTTGCGGAGCTGCTGCGGGCTGTCACAGAGATTGCGGAAACCTGGTGACCGGAAACAGAATGCCAACCCAAAGAGTCATCTTGCAAAACTGTCATTTATTGTGAATATCTTCACCATATGGGGTCACTTATGGTGAATATCTTTCTTCAGAGTTGCTGACTCTTGAAAACAGTGCAAAGATCACAGTTTTTAAAAGTTTTAAAAATTTAAGAATATTCACACAATCGTTTTTCTATGTGTGAGAGGGATTTGCACACTCTTATTTTTCTGTAAAATATTTCACATCCCAAATGTGAAGAAGTGAAAAAGACTTCGCAGCAGTCTTCATTGTGGTGCTCTTCATGATCATAGCCCCAGGAACCCTTGAGGTTCTTCTTCACAAGGCTGAGAGTGCTTCCTTCTTGAAGACGAGTGTCATTCATCACTTCAGTGATCCATGCATAGAATATGAAAATAAATTCTTCCAACTCATGGGATAAAGGGGACTCCCTTGAAGAATTTCATGTTTTTGGGCTGTATAGCTCTTTACAGAAAATGCACCTTTATAAATCACATGAATGTTAGTATTCTGGAAATGTCTTTTGTTAATATAATCTTCCCATGTTATTTAACAAATTGTTTTTGCACATATCTGATTATATTGAAAGCAGTTTTTTTGCATTCGAGTTTTAAACACTGTTATAAAATGTAGCCAAAGCTCACCTTTGAACAGATCCCGGTGACATTCTATTTCCAGGAAAATCCGGAACCTGATTTTAGTTCTGTGATTTTACACTTTTTACATGTGAGATTGGACAGTTTCAGAGGCCTTATTTTGTCATACTAAGTGTCTCCTGTAATTTTCAGGAAGATGATTTGTTCTTTCCAGAAGAGGAGACAAAAGCAAGATAGCCAAATGTGACATCAAGCTCCATTGTTTCGGAAATCCAGGATTTTGAATTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 29249, GC content failed 1009, low tm 15659, high tm 5873, high any compl 10, high end compl 46, high repeat similarity 60, long poly-x seq 43, ok 6549
PRIMER_RIGHT_EXPLAIN=considered 29276, GC content failed 1039, low tm 15745, high tm 5721, high end compl 12, high repeat similarity 25, long poly-x seq 40, ok 6694
PRIMER_PAIR_EXPLAIN=considered 995, unacceptable product size 930, high any compl 1, high end compl 8, ok 56
PRIMER_PAIR_PENALTY=0.0281
PRIMER_LEFT_PENALTY=0.017590
PRIMER_RIGHT_PENALTY=0.010529
PRIMER_LEFT_SEQUENCE=GGACAGTTTCAGAGGCCTTATTT
PRIMER_RIGHT_SEQUENCE=TGATGTCACATTTGGCTATCTTG
PRIMER_LEFT=2414,23
PRIMER_RIGHT=2527,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.011
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.0402
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.026007
PRIMER_LEFT_1_SEQUENCE=GATCCAAGTCAAGGCACTTTATG
PRIMER_RIGHT_1_SEQUENCE=ACCTTGTCATTCCAAAGTCTGAA
PRIMER_LEFT_1=337,23
PRIMER_RIGHT_1=1325,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=989
PRIMER_PAIR_PENALTY_2=0.0402
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.026007
PRIMER_LEFT_2_SEQUENCE=TTACCAGAAATCAGGCAGAACAT
PRIMER_RIGHT_2_SEQUENCE=ACCTTGTCATTCCAAAGTCTGAA
PRIMER_LEFT_2=549,23
PRIMER_RIGHT_2=1325,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=777
PRIMER_PAIR_PENALTY_3=0.0402
PRIMER_LEFT_3_PENALTY=0.014220
PRIMER_RIGHT_3_PENALTY=0.026007
PRIMER_LEFT_3_SEQUENCE=TCCAAGTCAAGGCACTTTATGAT
PRIMER_RIGHT_3_SEQUENCE=ACCTTGTCATTCCAAAGTCTGAA
PRIMER_LEFT_3=339,23
PRIMER_RIGHT_3=1325,23
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=987
PRIMER_PAIR_PENALTY_4=0.0518
PRIMER_LEFT_4_PENALTY=0.041315
PRIMER_RIGHT_4_PENALTY=0.010529
PRIMER_LEFT_4_SEQUENCE=TGGGGTCACTTATGGTGAATATC
PRIMER_RIGHT_4_SEQUENCE=TGATGTCACATTTGGCTATCTTG
PRIMER_LEFT_4=1740,23
PRIMER_RIGHT_4=2527,23
PRIMER_LEFT_4_TM=59.959
PRIMER_RIGHT_4_TM=60.011
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=788
PRIMER_PAIR_PENALTY_5=0.0554
PRIMER_LEFT_5_PENALTY=0.014220
PRIMER_RIGHT_5_PENALTY=0.041227
PRIMER_LEFT_5_SEQUENCE=GATCCAAGTCAAGGCACTTTATG
PRIMER_RIGHT_5_SEQUENCE=GGCATACACTCAGTAGCATTTCC
PRIMER_LEFT_5=337,23
PRIMER_RIGHT_5=1199,23
PRIMER_LEFT_5_TM=60.014
PRIMER_RIGHT_5_TM=60.041
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.2000
PRIMER_RIGHT_5_END_STABILITY=8.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=863
PRIMER_PAIR_PENALTY_6=0.0554
PRIMER_LEFT_6_PENALTY=0.014220
PRIMER_RIGHT_6_PENALTY=0.041227
PRIMER_LEFT_6_SEQUENCE=TTACCAGAAATCAGGCAGAACAT
PRIMER_RIGHT_6_SEQUENCE=GGCATACACTCAGTAGCATTTCC
PRIMER_LEFT_6=549,23
PRIMER_RIGHT_6=1199,23
PRIMER_LEFT_6_TM=60.014
PRIMER_RIGHT_6_TM=60.041
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=651
PRIMER_PAIR_PENALTY_7=0.0554
PRIMER_LEFT_7_PENALTY=0.014220
PRIMER_RIGHT_7_PENALTY=0.041227
PRIMER_LEFT_7_SEQUENCE=TCCAAGTCAAGGCACTTTATGAT
PRIMER_RIGHT_7_SEQUENCE=GGCATACACTCAGTAGCATTTCC
PRIMER_LEFT_7=339,23
PRIMER_RIGHT_7=1199,23
PRIMER_LEFT_7_TM=60.014
PRIMER_RIGHT_7_TM=60.041
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.5000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=861
PRIMER_PAIR_PENALTY_8=0.0589
PRIMER_LEFT_8_PENALTY=0.041315
PRIMER_RIGHT_8_PENALTY=0.017590
PRIMER_LEFT_8_SEQUENCE=TGGGGTCACTTATGGTGAATATC
PRIMER_RIGHT_8_SEQUENCE=AAATAAGGCCTCTGAAACTGTCC
PRIMER_LEFT_8=1740,23
PRIMER_RIGHT_8=2436,23
PRIMER_LEFT_8_TM=59.959
PRIMER_RIGHT_8_TM=60.018
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.5000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=697
PRIMER_PAIR_PENALTY_9=0.0625
PRIMER_LEFT_9_PENALTY=0.036444
PRIMER_RIGHT_9_PENALTY=0.026007
PRIMER_LEFT_9_SEQUENCE=GCTTAATCCCAAGCAACTATGTG
PRIMER_RIGHT_9_SEQUENCE=ACCTTGTCATTCCAAAGTCTGAA
PRIMER_LEFT_9=474,23
PRIMER_RIGHT_9=1325,23
PRIMER_LEFT_9_TM=60.036
PRIMER_RIGHT_9_TM=60.026
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=852
PRIMER_PAIR_PENALTY_10=0.0777
PRIMER_LEFT_10_PENALTY=0.036444
PRIMER_RIGHT_10_PENALTY=0.041227
PRIMER_LEFT_10_SEQUENCE=GCTTAATCCCAAGCAACTATGTG
PRIMER_RIGHT_10_SEQUENCE=GGCATACACTCAGTAGCATTTCC
PRIMER_LEFT_10=474,23
PRIMER_RIGHT_10=1199,23
PRIMER_LEFT_10_TM=60.036
PRIMER_RIGHT_10_TM=60.041
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=8.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=726
PRIMER_PAIR_PENALTY_11=0.0965
PRIMER_LEFT_11_PENALTY=0.082291
PRIMER_RIGHT_11_PENALTY=0.014220
PRIMER_LEFT_11_SEQUENCE=CCTTTCCTCCTATAACACCATCC
PRIMER_RIGHT_11_SEQUENCE=ATCATAAAGTGCCTTGACTTGGA
PRIMER_LEFT_11=91,23
PRIMER_RIGHT_11=361,23
PRIMER_LEFT_11_TM=60.082
PRIMER_RIGHT_11_TM=60.014
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=8.5000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=271
PRIMER_PAIR_PENALTY_12=0.0974
PRIMER_LEFT_12_PENALTY=0.014220
PRIMER_RIGHT_12_PENALTY=0.083165
PRIMER_LEFT_12_SEQUENCE=GATCCAAGTCAAGGCACTTTATG
PRIMER_RIGHT_12_SEQUENCE=TCTCCAGATATTCCATTCCTTCA
PRIMER_LEFT_12=337,23
PRIMER_RIGHT_12=1232,23
PRIMER_LEFT_12_TM=60.014
PRIMER_RIGHT_12_TM=59.917
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=896
PRIMER_PAIR_PENALTY_13=0.0974
PRIMER_LEFT_13_PENALTY=0.014220
PRIMER_RIGHT_13_PENALTY=0.083165
PRIMER_LEFT_13_SEQUENCE=TTACCAGAAATCAGGCAGAACAT
PRIMER_RIGHT_13_SEQUENCE=TCTCCAGATATTCCATTCCTTCA
PRIMER_LEFT_13=549,23
PRIMER_RIGHT_13=1232,23
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=59.917
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=684
PRIMER_PAIR_PENALTY_14=0.0974
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.083165
PRIMER_LEFT_14_SEQUENCE=TCCAAGTCAAGGCACTTTATGAT
PRIMER_RIGHT_14_SEQUENCE=TCTCCAGATATTCCATTCCTTCA
PRIMER_LEFT_14=339,23
PRIMER_RIGHT_14=1232,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=59.917
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.5000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=894
PRIMER_PAIR_PENALTY_15=0.1196
PRIMER_LEFT_15_PENALTY=0.036444
PRIMER_RIGHT_15_PENALTY=0.083165
PRIMER_LEFT_15_SEQUENCE=GCTTAATCCCAAGCAACTATGTG
PRIMER_RIGHT_15_SEQUENCE=TCTCCAGATATTCCATTCCTTCA
PRIMER_LEFT_15=474,23
PRIMER_RIGHT_15=1232,23
PRIMER_LEFT_15_TM=60.036
PRIMER_RIGHT_15_TM=59.917
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=759
PRIMER_PAIR_PENALTY_16=0.1553
PRIMER_LEFT_16_PENALTY=0.129292
PRIMER_RIGHT_16_PENALTY=0.026007
PRIMER_LEFT_16_SEQUENCE=AGCTGGGTTTAGCTACGAAAAGT
PRIMER_RIGHT_16_SEQUENCE=ACCTTGTCATTCCAAAGTCTGAA
PRIMER_LEFT_16=853,23
PRIMER_RIGHT_16=1325,23
PRIMER_LEFT_16_TM=59.871
PRIMER_RIGHT_16_TM=60.026
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=473
PRIMER_PAIR_PENALTY_17=0.1576
PRIMER_LEFT_17_PENALTY=0.014220
PRIMER_RIGHT_17_PENALTY=0.143370
PRIMER_LEFT_17_SEQUENCE=GATCCAAGTCAAGGCACTTTATG
PRIMER_RIGHT_17_SEQUENCE=TAATTTCATCATCACTTTGGCCT
PRIMER_LEFT_17=337,23
PRIMER_RIGHT_17=1048,23
PRIMER_LEFT_17_TM=60.014
PRIMER_RIGHT_17_TM=59.857
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=34.783
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.2000
PRIMER_RIGHT_17_END_STABILITY=10.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=712
PRIMER_PAIR_PENALTY_18=0.1576
PRIMER_LEFT_18_PENALTY=0.014220
PRIMER_RIGHT_18_PENALTY=0.143370
PRIMER_LEFT_18_SEQUENCE=TTACCAGAAATCAGGCAGAACAT
PRIMER_RIGHT_18_SEQUENCE=TAATTTCATCATCACTTTGGCCT
PRIMER_LEFT_18=549,23
PRIMER_RIGHT_18=1048,23
PRIMER_LEFT_18_TM=60.014
PRIMER_RIGHT_18_TM=59.857
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=10.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=500
PRIMER_PAIR_PENALTY_19=0.1691
PRIMER_LEFT_19_PENALTY=0.014220
PRIMER_RIGHT_19_PENALTY=0.154904
PRIMER_LEFT_19_SEQUENCE=TTACCAGAAATCAGGCAGAACAT
PRIMER_RIGHT_19_SEQUENCE=ACCTAAATGGACCACTCCAAACT
PRIMER_LEFT_19=549,23
PRIMER_RIGHT_19=949,23
PRIMER_LEFT_19_TM=60.014
PRIMER_RIGHT_19_TM=60.155
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1c (MLT1c subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=401
=