PRIMER_SEQUENCE_ID=NM_003331
SEQUENCE=GACGCGGGCGCGGAAGGAGCGCGGCCGGAGGTCCTCAGGAAGAAGCCGCGGGGACTGGCTGCGCTTGACAGGCTGCACTTGGATGGGAGCACCTGGTGCCTCGGGACTGCTCCGATGCCCGGGTCTGTGCTGAATGTGTAATATGCGGAACTATATTGAAACATTACAACCATCTTTTGATGGCAACACCCTGAGGACCTCCCTTTTCCAGATGGGGAAACTGAGGCCCAGAATTGCTAAGTGGCTTGCTTGAGTTGACACAGGGAGCTCCAGGACTCACCCTCAGCTGAGCCACCTGCCGGGAGCATGCCTCTGCGCCACTGGGGGATGGCCAGGGGCAGTAAGCCCGTTGGGGATGGAGCCCAGCCCATGGCTGCCATGGGAGGCCTGAAGGTGCTTCTGCACTGGGCTGGTCCAGGCGGCGGGGAGCCCTGGGTCACTTTCAGTGAGTCATCGCTGACAGCTGAGGAAGTCTGCATCCACATTGCACATAAAGTTGGTATCACTCCTCCTTGCTTCAATCTCTTTGCCCTCTTCGATGCTCAGGCCCAAGTCTGGTTGCCCCCAAACCACATCCTAGAGATCCCCAGAGATGCAAGCCTGATGCTATATTTCCGCATAAGGTTTTATTTCCGGAACTGGCATGGCATGAATCCTCGGGAACCGGCTGTGTACCGTTGTGGGCCCCCAGGAACCGAGGCATCCTCAGATCAGACAGCACAGGGGATGCAACTCCTGGACCCAGCCTCATTTGAGTACCTCTTTGAGCAGGGCAAGCATGAGTTTGTGAATGACGTGGCATCACTGTGGGAGCTGTCGACCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCCTGGGCATGGCCTTTCTGCACCTCTGTCACCTCGCTCTCCGCCATGGCATCCCCCTGGAGGAGGTGGCCAAGAAGACCAGCTTCAAGGACTGCATCCCGCGCTCCTTCCGCCGGCATATCCGGCAGCACAGCGCCCTGACCCGGCTGCGCCTTCGGAACGTCTTCCGCAGGTTCCTGCGGGACTTCCAGCCGGGCCGACTCTCCCAGCAGATGGTCATGGTCAAATACCTAGCCACACTCGAGCGGCTGGCACCCCGCTTCGGCACAGAGCGTGTGCCCGTGTGCCACCTGAGGCTGCTGGCCCAGGCCGAGGGGGAGCCCTGCTACATCCGGGACAGTGGGGTGGCCCCTACAGACCCTGGCCCTGAGTCTGCTGCTGGGCCCCCAACCCACGAGGTGCTGGTGACAGGCACTGGTGGCATCCAGTGGTGGCCAGTAGAGGAGGAGGTGAACAAGGAGGAGGGTTCTAGTGGCAGCAGTGGCAGGAACCCCCAAGCCAGCCTGTTTGGGAAGAAGGCCAAGGCTCACAAGGCATTCGGCCAGCCGGCAGACAGGCCGCGGGAGCCACTGTGGGCCTACTTCTGTGACTTCCGGGACATCACCCACGTGGTGCTGAAAGAGCACTGTGTCAGCATCCACCGGCAGGACAACAAGTGCCTGGAGCTGAGCTTGCCTTCCCGGGCTGCGGCGCTGTCCTTCGTGTCGCTGGTGGACGGCTATTTCCGCCTGACGGCCGACTCCAGCCACTACCTGTGCCACGAGGTGGCTCCCCCACGGCTGGTGATGAGCATCCGGGATGGGATCCACGGACCCCTGCTGGAGCCATTTGTGCAGGCCAAGCTGCGGCCCGAGGACGGCCTGTACCTCATTCACTGGAGCACCAGCCACCCCTACCGCCTGATCCTCACAGTGGCCCAGCGTAGCCAGGCACCAGACGGCATGCAGAGCTTGCGGCTCCGAAAGTTCCCCATTGAGCAGCAGGACGGGGCCTTCGTGCTGGAGGGCTGGGGCCGGTCCTTCCCCAGCGTTCGGGAACTTGGGGCTGCCTTGCAGGGCTGCTTGCTGAGGGCCGGGGATGACTGCTTCTCTCTGCGTCGCTGTTGCCTGCCCCAACCAGGAGAAACCTCCAATCTCATCATCATGCGGGGGGCTCGGGCCAGCCCCAGGACACTCAACCTCAGCCAGCTCAGCTTCCACCGGGTTGACCAGAAGGAGATCACCCAGCTGTCCCACTTGGGCCAGGGCACAAGGACCAACGTGTATGAGGGCCGCCTGCGAGTGGAGGGCAGCGGGGACCCTGAGGAGGGCAAGATGGATGACGAGGACCCCCTCGTGCCTGGCAGGGACCGTGGGCAGGAGCTACGAGTGGTGCTCAAAGTGCTGGACCCTAGTCACCATGACATCGCCCTGGCCTTCTACGAGACAGCCAGCCTCATGAGCCAGGTCTCCCACACGCACCTGGCCTTCGTGCATGGCGTCTGTGTGCGCGGCCCTGAAAATAGCATGGTGACAGAGTACGTGGAGCACGGACCCCTGGATGTGTGGCTGCGGAGGGAGCGGGGCCATGTGCCCATGGCTTGGAAGATGGTGGTGGCCCAGCAGCTGGCCAGCGCCCTCAGCTACCTGGAGAACAAGAACCTGGTTCATGGTAATGTGTGTGGCCGGAACATCCTGCTGGCCCGGCTGGGGTTGGCAGAGGGCACCAGCCCCTTCATCAAGCTGAGTGATCCTGGCGTGGGCCTGGGCGCCCTCTCCAGGGAGGAGCGGGTGGAGAGGATCCCCTGGCTGGCCCCCGAATGCCTACCAGGTGGGGCCAACAGCCTAAGCACCGCCATGGACAAGTGGGGGTTTGGCGCCACCCTCCTGGAGATCTGCTTTGACGGAGAGGCCCCTCTGCAGAGCCGCAGTCCCTCCGAGAAGGAGCATTTCTACCAGAGGCAGCACCGGCTGCCCGAGCCCTCCTGCCCACAGCTGGCCACACTCACCAGCCAGTGTCTGACCTATGAGCCAACCCAGAGGCCATCATTCCGCACCATCCTGCGTGACCTCACCCGCGTGCAGCCCCACAATCTTGCTGACGTCTTGACTGTGAACCGGGACTCACCGGCCGTCGGACCTACTACTTTCCACAAGCGCTATTTGAAAAAGATCCGAGATCTGGGCGAGGGTCACTTCGGCAAGGTCAGCTTGTACTGCTACGATCCGACCAACGACGGCACTGGCGAGATGGTGGCGGTGAAAGCCCTCAAGGCAGACTGCGGCCCCCAGCACCGCTCGGGCTGGAAGCAGGAGATTGACATTCTGCGCACGCTCTACCACGAGCACATCATCAAGTACAAGGGCTGCTGCGAGGACCAAGGCGAGAAGTCGCTGCAGCTGGTCATGGAGTACGTGCCCCTGGGCAGCCTCCGAGACTACCTGCCCCGGCACAGCATCGGGCTGGCCCAGCTGCTGCTCTTCGCCCAGCAGATCTGCGAGGGCATGGCCTATCTGCACGCGCACGACTACATCCACCGAGACCTAGCCGCGCGCAACGTGCTGCTGGACAACGACAGGCTGGTCAAGATCGGGGACTTTGGCCTAGCCAAGGCCGTGCCCGAAGGCCACGAGTACTACCGCGTGCGCGAGGATGGGGACAGCCCCGTGTTCTGGTATGCCCCAGAGTGCCTGAAGGAGTATAAGTTCTACTATGCGTCAGATGTCTGGTCCTTCGGGGTGACCCTGTATGAGCTGCTGACGCACTGTGACTCCAGCCAGAGCCCCCCCACGAAATTCCTTGAGCTCATAGGCATTGCTCAGGGTCAGATGACAGTTCTGAGACTCACTGAGTTGCTGGAACGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGGTCTATCATCTCATGAAGAACTGCTGGGAGACAGAGGCGTCCTTTCGCCCAACCTTCGAGAACCTCATACCCATTCTGAAGACAGTCCATGAGAAGTACCAAGGCCAGGCCCCTTCAGTGTTCAGCGTGTGCTGAGGCACAATGGCAGCCCTGCCTGGGAGGACTGGACCAGGCAGTGGCTGCAGAGGGAGCCTCCTGCTCCCTGCTCCAGGATGAAACCAAGAGGGGGATGTCAGCCTCACCCACACCGTGTGCCTTACTCCTGTCTAGAGACCCCACCTCTGTGAACTTATTTTTCTTTCTTGGCCGTGAGCCTAACCATGATCTTGAGGGACCCAACATTTGTAGGGGCACTAATCCAGCCCTTAAATCCCCCAGCTTCCAAACTTGAGGCCCACCATCTCCACCATCTGGTAATAAACTCATGTTTTCTCTGCTGGG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 48641, GC content failed 1182, low tm 9822, high tm 29051, high any compl 17, high end compl 21, high repeat similarity 32, long poly-x seq 37, ok 8479
PRIMER_RIGHT_EXPLAIN=considered 48629, GC content failed 1026, low tm 10126, high tm 28781, high any compl 5, high end compl 6, high repeat similarity 13, long poly-x seq 39, ok 8633
PRIMER_PAIR_EXPLAIN=considered 829, unacceptable product size 758, high end compl 6, ok 65
PRIMER_PAIR_PENALTY=0.0979
PRIMER_LEFT_PENALTY=0.016074
PRIMER_RIGHT_PENALTY=0.081865
PRIMER_LEFT_SEQUENCE=AACTTATTTTTCTTTCTTGGCCG
PRIMER_RIGHT_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT=4021,23
PRIMER_RIGHT=4154,23
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1310
PRIMER_LEFT_1_PENALTY=0.008837
PRIMER_RIGHT_1_PENALTY=0.122120
PRIMER_LEFT_1_SEQUENCE=TGTCCCTGTGAGGTCTATCATCT
PRIMER_RIGHT_1_SEQUENCE=TGGACTGTCTTCAGAATGGGTAT
PRIMER_LEFT_1=3723,23
PRIMER_RIGHT_1=3823,23
PRIMER_LEFT_1_TM=60.009
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.1637
PRIMER_LEFT_2_PENALTY=0.008837
PRIMER_RIGHT_2_PENALTY=0.154904
PRIMER_LEFT_2_SEQUENCE=TGTCCCTGTGAGGTCTATCATCT
PRIMER_RIGHT_2_SEQUENCE=AAATAAGTTCACAGAGGTGGGGT
PRIMER_LEFT_2=3723,23
PRIMER_RIGHT_2=4029,23
PRIMER_LEFT_2_TM=60.009
PRIMER_RIGHT_2_TM=60.155
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=307
PRIMER_PAIR_PENALTY_3=0.2040
PRIMER_LEFT_3_PENALTY=0.122120
PRIMER_RIGHT_3_PENALTY=0.081865
PRIMER_LEFT_3_SEQUENCE=CAAATGTCCCTGTGAGGTCTATC
PRIMER_RIGHT_3_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT_3=3719,23
PRIMER_RIGHT_3=4154,23
PRIMER_LEFT_3_TM=59.878
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=436
PRIMER_PAIR_PENALTY_4=0.2040
PRIMER_LEFT_4_PENALTY=0.122120
PRIMER_RIGHT_4_PENALTY=0.081865
PRIMER_LEFT_4_SEQUENCE=ATACCCATTCTGAAGACAGTCCA
PRIMER_RIGHT_4_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT_4=3801,23
PRIMER_RIGHT_4=4154,23
PRIMER_LEFT_4_TM=59.878
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=354
PRIMER_PAIR_PENALTY_5=0.2082
PRIMER_LEFT_5_PENALTY=0.008837
PRIMER_RIGHT_5_PENALTY=0.199411
PRIMER_LEFT_5_SEQUENCE=TGTCCCTGTGAGGTCTATCATCT
PRIMER_RIGHT_5_SEQUENCE=GGGTCTCTAGACAGGAGTAAGGC
PRIMER_LEFT_5=3723,23
PRIMER_RIGHT_5=4010,23
PRIMER_LEFT_5_TM=60.009
PRIMER_RIGHT_5_TM=59.801
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=56.522
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=288
PRIMER_PAIR_PENALTY_6=0.2154
PRIMER_LEFT_6_PENALTY=0.091943
PRIMER_RIGHT_6_PENALTY=0.123491
PRIMER_LEFT_6_SEQUENCE=ACTCCTCCTTGCTTCAATCTCTT
PRIMER_RIGHT_6_SEQUENCE=AGGTATTTGACCATGACCATCTG
PRIMER_LEFT_6=504,23
PRIMER_RIGHT_6=1084,23
PRIMER_LEFT_6_TM=59.908
PRIMER_RIGHT_6_TM=60.123
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=581
PRIMER_PAIR_PENALTY_7=0.2368
PRIMER_LEFT_7_PENALTY=0.154904
PRIMER_RIGHT_7_PENALTY=0.081865
PRIMER_LEFT_7_SEQUENCE=ACCCCACCTCTGTGAACTTATTT
PRIMER_RIGHT_7_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT_7=4007,23
PRIMER_RIGHT_7=4154,23
PRIMER_LEFT_7_TM=60.155
PRIMER_RIGHT_7_TM=60.082
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=6.2000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=7.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=148
PRIMER_PAIR_PENALTY_8=0.2442
PRIMER_LEFT_8_PENALTY=0.122120
PRIMER_RIGHT_8_PENALTY=0.122120
PRIMER_LEFT_8_SEQUENCE=CAAATGTCCCTGTGAGGTCTATC
PRIMER_RIGHT_8_SEQUENCE=TGGACTGTCTTCAGAATGGGTAT
PRIMER_LEFT_8=3719,23
PRIMER_RIGHT_8=3823,23
PRIMER_LEFT_8_TM=59.878
PRIMER_RIGHT_8_TM=59.878
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=6.8000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=105
PRIMER_PAIR_PENALTY_9=0.2770
PRIMER_LEFT_9_PENALTY=0.122120
PRIMER_RIGHT_9_PENALTY=0.154904
PRIMER_LEFT_9_SEQUENCE=CAAATGTCCCTGTGAGGTCTATC
PRIMER_RIGHT_9_SEQUENCE=AAATAAGTTCACAGAGGTGGGGT
PRIMER_LEFT_9=3719,23
PRIMER_RIGHT_9=4029,23
PRIMER_LEFT_9_TM=59.878
PRIMER_RIGHT_9_TM=60.155
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.6000
PRIMER_RIGHT_9_END_STABILITY=10.6000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=311
PRIMER_PAIR_PENALTY_10=0.2770
PRIMER_LEFT_10_PENALTY=0.122120
PRIMER_RIGHT_10_PENALTY=0.154904
PRIMER_LEFT_10_SEQUENCE=ATACCCATTCTGAAGACAGTCCA
PRIMER_RIGHT_10_SEQUENCE=AAATAAGTTCACAGAGGTGGGGT
PRIMER_LEFT_10=3801,23
PRIMER_RIGHT_10=4029,23
PRIMER_LEFT_10_TM=59.878
PRIMER_RIGHT_10_TM=60.155
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=10.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=229
PRIMER_PAIR_PENALTY_11=0.2813
PRIMER_LEFT_11_PENALTY=0.199411
PRIMER_RIGHT_11_PENALTY=0.081865
PRIMER_LEFT_11_SEQUENCE=GCCTTACTCCTGTCTAGAGACCC
PRIMER_RIGHT_11_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT_11=3988,23
PRIMER_RIGHT_11=4154,23
PRIMER_LEFT_11_TM=59.801
PRIMER_RIGHT_11_TM=60.082
PRIMER_LEFT_11_GC_PERCENT=56.522
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.1000
PRIMER_RIGHT_11_END_STABILITY=8.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=167
PRIMER_PAIR_PENALTY_12=0.2908
PRIMER_LEFT_12_PENALTY=0.208920
PRIMER_RIGHT_12_PENALTY=0.081865
PRIMER_LEFT_12_SEQUENCE=CCAGAGTGCCTGAAGGAGTATAA
PRIMER_RIGHT_12_SEQUENCE=TTATTACCAGATGGTGGAGATGG
PRIMER_LEFT_12=3513,23
PRIMER_RIGHT_12=4154,23
PRIMER_LEFT_12_TM=59.791
PRIMER_RIGHT_12_TM=60.082
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=5.2000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=642
PRIMER_PAIR_PENALTY_13=0.2949
PRIMER_LEFT_13_PENALTY=0.008837
PRIMER_RIGHT_13_PENALTY=0.286105
PRIMER_LEFT_13_SEQUENCE=TGTCCCTGTGAGGTCTATCATCT
PRIMER_RIGHT_13_SEQUENCE=ATAAGTTCACAGAGGTGGGGTCT
PRIMER_LEFT_13=3723,23
PRIMER_RIGHT_13=4027,23
PRIMER_LEFT_13_TM=60.009
PRIMER_RIGHT_13_TM=60.286
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER11B a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.6000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=305
PRIMER_PAIR_PENALTY_14=0.3064
PRIMER_LEFT_14_PENALTY=0.045831
PRIMER_RIGHT_14_PENALTY=0.260543
PRIMER_LEFT_14_SEQUENCE=AGAAACCTCCAATCTCATCATCA
PRIMER_RIGHT_14_SEQUENCE=CCTCTGGTAGAAATGCTCCTTCT
PRIMER_LEFT_14=1973,23
PRIMER_RIGHT_14=2792,23
PRIMER_LEFT_14_TM=59.954
PRIMER_RIGHT_14_TM=60.261
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=820
PRIMER_PAIR_PENALTY_15=0.3073
PRIMER_LEFT_15_PENALTY=0.185154
PRIMER_RIGHT_15_PENALTY=0.122120
PRIMER_LEFT_15_SEQUENCE=CACAAGCGCTATTTGAAAAAGAT
PRIMER_RIGHT_15_SEQUENCE=GATAGACCTCACAGGGACATTTG
PRIMER_LEFT_15=2982,23
PRIMER_RIGHT_15=3741,23
PRIMER_LEFT_15_TM=59.815
PRIMER_RIGHT_15_TM=59.878
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=760
PRIMER_PAIR_PENALTY_16=0.3073
PRIMER_LEFT_16_PENALTY=0.185154
PRIMER_RIGHT_16_PENALTY=0.122120
PRIMER_LEFT_16_SEQUENCE=CACAAGCGCTATTTGAAAAAGAT
PRIMER_RIGHT_16_SEQUENCE=TGATAGACCTCACAGGGACATTT
PRIMER_LEFT_16=2982,23
PRIMER_RIGHT_16=3742,23
PRIMER_LEFT_16_TM=59.815
PRIMER_RIGHT_16_TM=59.878
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=761
PRIMER_PAIR_PENALTY_17=0.3073
PRIMER_LEFT_17_PENALTY=0.185154
PRIMER_RIGHT_17_PENALTY=0.122120
PRIMER_LEFT_17_SEQUENCE=CACAAGCGCTATTTGAAAAAGAT
PRIMER_RIGHT_17_SEQUENCE=TGGACTGTCTTCAGAATGGGTAT
PRIMER_LEFT_17=2982,23
PRIMER_RIGHT_17=3823,23
PRIMER_LEFT_17_TM=59.815
PRIMER_RIGHT_17_TM=59.878
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.8000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=842
PRIMER_PAIR_PENALTY_18=0.3215
PRIMER_LEFT_18_PENALTY=0.122120
PRIMER_RIGHT_18_PENALTY=0.199411
PRIMER_LEFT_18_SEQUENCE=CAAATGTCCCTGTGAGGTCTATC
PRIMER_RIGHT_18_SEQUENCE=GGGTCTCTAGACAGGAGTAAGGC
PRIMER_LEFT_18=3719,23
PRIMER_RIGHT_18=4010,23
PRIMER_LEFT_18_TM=59.878
PRIMER_RIGHT_18_TM=59.801
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=56.522
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=292
PRIMER_PAIR_PENALTY_19=0.3215
PRIMER_LEFT_19_PENALTY=0.122120
PRIMER_RIGHT_19_PENALTY=0.199411
PRIMER_LEFT_19_SEQUENCE=ATACCCATTCTGAAGACAGTCCA
PRIMER_RIGHT_19_SEQUENCE=GGGTCTCTAGACAGGAGTAAGGC
PRIMER_LEFT_19=3801,23
PRIMER_RIGHT_19=4010,23
PRIMER_LEFT_19_TM=59.878
PRIMER_RIGHT_19_TM=59.801
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=56.522
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=9.7000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=210
=