PRIMER_SEQUENCE_ID=NM_003985
SEQUENCE=GAGTCGCCGCTTCCGCCTTGACCAGGTGGAGCTGGAGACCTGGTCTCTCTAGGGCCTGCCCTGAGCTCACCATCTGAAGGAGAGTGCCATCATCCTTAGGAACTCCTTCTCCAGACATGCTTCCCGAGGCTGGCTCCCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCCCAGTTTTACTGGCCCATCCTAGAGGAGCTTAATGTCACCCGGCCAGGGCACTTCGACTTTGTAAAGCCTGAGGACCTGGACGGCATTGGCATGGGCCGGCCTGCCCAACGCAGACTGTCCGAAGCTCTGAAAAGCCTACGTTCTGGGCCTAAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCACGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTGCAGAGGGGAGCTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGCAAGAGTGTCCCAGTGGCTGTCAAGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGGACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGTACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACGCGCGCTTAACGGCCCCGGCCCCGACACCCCCGCTGCTCGTGGCCCTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGGCGTACCTGGGGGCCCGCGGGCTGGTGCACCGAGACCTCGCTACGCGCAACCTACAGCTGGCGTCGCCGCGCACCATCAAGGTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGCGGGCCCCGCCCTATCCCCTACACCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTCTCGTCTGCCTCGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGCGAGGAACCCTGGCCCGGGGTCCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCCCGGCTGCCTAGGCCTCCCCCCTCCTCCAGGGCCCTCTACTCCCTCGCCTTGCGCTGCTGGGCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGGAGGGGCTGCTGCAAGAGGCCGGGCCTTCGGAAGCATGTTGTGTGAGGGATGCCACAGAACCAGGCGCCCTGAGGATGGAGACTGGTGACCCCATCACAGTCATCGAGGGCAGCTCCTCTTTCCACAGCCCCGACTCCACAATCTGGAAGGACCAGAATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGACAGATGCGGGGGGCTTGCCAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACACCCAGGAAGCATAGATGGAGACAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGGGGCCAGAGGAGGAACATGCCCCTGGAGAGGATGAAAGGCATTTCCAGGAGTCTGGAGTCAGTTCTGTCCCTCGGTCCTCGTCCCACAGGGGGTGGTTCAAGCCCCCCTGAAATTCGACAAGCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCCTCCACGCCCACCTTTATCCTCTAGCTCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCCGAAAGAAAACCCCCACACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAGGCCTCTTGTCCGATCCTGAGTTGCAGAGGAAGATTATGGAAATGGAGCTGAGTGTGCATTGGGTCACCCACCAGGAGTGCCAGACAGCACTAGGAGCCACTGGGGGAGATGTGGCTTCTGCCATCCGGAACCTCAAGGTAGATCAGCTCTTCCTCCTGAGTAGCCGGTCCAGAGCTGACTGCTGGCGCATCCTGGAGCATTACCAGTGGGACCTCTCAGCTGCCAGCCGTTATGTCCTGGCCAGGCCCTGAGCCCAGCTTCTGCGGGCACAGACACCAGCATGAAAAGCCTAGGTCCCTGAGGGCCTGGCCACATGGGACCAAGTGGAACCAGAACAAGGCCCCGACAGGGGTAGACGTTCCACTTGTGGAGATCCCACCTGCCGCTAGGCACGTGGAGGAGGAGCCCAGAGTTGGGCACTGGCAAATGTCTCCTCCCTCCCATGCTCCTTGGCTTCTGAAGGCTGAAGCCCCTTTGGCTGGGCCAAGAAGGATCTAGTCTGCCCACTACATTCTCAAACAAGAGGACTTGGAGGAAAAGAGCTACTATACATCATATGCAGAGGAAGCTTCTACGCGCTAGAGAGGATCAAGGGGCCACACTGGACCATGTGAACAGCCATCCGGAACTGCCATCAGCTACCACACTGGACTCTGCAGGGCAGCCATCCTGGATGATGGAAGCCACCATATTGACCTGGGGTATAGGCCCAAACTGCCTTCGTTTGGTCCAGGGCCATCGTGGGTGATGACGATTGCTCTCTTGCACTCATGGACATTTGATGCTGGTAGTATGGATTATGAGATGGACTAGCCCCTGCTCCAGCCCAGTTCTCACATTCCCCTTTGTTTTTTCCCATACCAACTGCTTCTACCCTCCCCTATTACATACATCTTTCAATGTCC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31343, GC content failed 1041, low tm 6655, high tm 17892, high any compl 37, high end compl 61, high repeat similarity 82, long poly-x seq 97, ok 5478
PRIMER_RIGHT_EXPLAIN=considered 31170, GC content failed 1035, low tm 6794, high tm 17589, high end compl 6, high repeat similarity 8, long poly-x seq 118, ok 5620
PRIMER_PAIR_EXPLAIN=considered 1536, unacceptable product size 1464, high end compl 11, ok 61
PRIMER_PAIR_PENALTY=0.0576
PRIMER_LEFT_PENALTY=0.037017
PRIMER_RIGHT_PENALTY=0.020535
PRIMER_LEFT_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_SEQUENCE=CACTCAGCTCCATTTCCATAATC
PRIMER_LEFT=1615,23
PRIMER_RIGHT=1919,23
PRIMER_LEFT_TM=59.963
PRIMER_RIGHT_TM=59.979
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=305
PRIMER_PAIR_PENALTY_1=0.1022
PRIMER_LEFT_1_PENALTY=0.091795
PRIMER_RIGHT_1_PENALTY=0.010384
PRIMER_LEFT_1_SEQUENCE=TTCTCAAACAAGAGGACTTGGAG
PRIMER_RIGHT_1_SEQUENCE=TAGAAGCAGTTGGTATGGGAAAA
PRIMER_LEFT_1=2377,23
PRIMER_RIGHT_1=2738,23
PRIMER_LEFT_1_TM=59.908
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=362
PRIMER_PAIR_PENALTY_2=0.1552
PRIMER_LEFT_2_PENALTY=0.002035
PRIMER_RIGHT_2_PENALTY=0.153194
PRIMER_LEFT_2_SEQUENCE=CCCATCCTAGAGGAGCTTAATGT
PRIMER_RIGHT_2_SEQUENCE=GTTCATCATGACCGATACCTCTC
PRIMER_LEFT_2=188,23
PRIMER_RIGHT_2=637,23
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=450
PRIMER_PAIR_PENALTY_3=0.1611
PRIMER_LEFT_3_PENALTY=0.150712
PRIMER_RIGHT_3_PENALTY=0.010384
PRIMER_LEFT_3_SEQUENCE=CATTCTCAAACAAGAGGACTTGG
PRIMER_RIGHT_3_SEQUENCE=TAGAAGCAGTTGGTATGGGAAAA
PRIMER_LEFT_3=2375,23
PRIMER_RIGHT_3=2738,23
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=60.010
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=364
PRIMER_PAIR_PENALTY_4=0.1877
PRIMER_LEFT_4_PENALTY=0.037017
PRIMER_RIGHT_4_PENALTY=0.150712
PRIMER_LEFT_4_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_4_SEQUENCE=CCAAGTCCTCTTGTTTGAGAATG
PRIMER_LEFT_4=1615,23
PRIMER_RIGHT_4=2397,23
PRIMER_LEFT_4_TM=59.963
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=783
PRIMER_PAIR_PENALTY_5=0.2156
PRIMER_LEFT_5_PENALTY=0.205235
PRIMER_RIGHT_5_PENALTY=0.010384
PRIMER_LEFT_5_SEQUENCE=ACCATATTGACCTGGGGTATAGG
PRIMER_RIGHT_5_SEQUENCE=TAGAAGCAGTTGGTATGGGAAAA
PRIMER_LEFT_5=2551,23
PRIMER_RIGHT_5=2738,23
PRIMER_LEFT_5_TM=60.205
PRIMER_RIGHT_5_TM=60.010
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.1000
PRIMER_RIGHT_5_END_STABILITY=7.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=188
PRIMER_PAIR_PENALTY_6=0.2364
PRIMER_LEFT_6_PENALTY=0.037017
PRIMER_RIGHT_6_PENALTY=0.199411
PRIMER_LEFT_6_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_6_SEQUENCE=AGTGGGCAGACTAGATCCTTCTT
PRIMER_LEFT_6=1615,23
PRIMER_RIGHT_6=2373,23
PRIMER_LEFT_6_TM=59.963
PRIMER_RIGHT_6_TM=59.801
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=759
PRIMER_PAIR_PENALTY_7=0.2420
PRIMER_LEFT_7_PENALTY=0.088826
PRIMER_RIGHT_7_PENALTY=0.153194
PRIMER_LEFT_7_SEQUENCE=AAGCTCTGAAAAGCCTACGTTCT
PRIMER_RIGHT_7_SEQUENCE=GTTCATCATGACCGATACCTCTC
PRIMER_LEFT_7=297,23
PRIMER_RIGHT_7=637,23
PRIMER_LEFT_7_TM=60.089
PRIMER_RIGHT_7_TM=59.847
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.4000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=341
PRIMER_PAIR_PENALTY_8=0.2423
PRIMER_LEFT_8_PENALTY=0.037017
PRIMER_RIGHT_8_PENALTY=0.205235
PRIMER_LEFT_8_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_8_SEQUENCE=CCTATACCCCAGGTCAATATGGT
PRIMER_LEFT_8=1615,23
PRIMER_RIGHT_8=2573,23
PRIMER_LEFT_8_TM=59.963
PRIMER_RIGHT_8_TM=60.205
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=7.8000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=959
PRIMER_PAIR_PENALTY_9=0.2467
PRIMER_LEFT_9_PENALTY=0.091795
PRIMER_RIGHT_9_PENALTY=0.154909
PRIMER_LEFT_9_SEQUENCE=TTCTCAAACAAGAGGACTTGGAG
PRIMER_RIGHT_9_SEQUENCE=AGGGGCTAGTCCATCTCATAATC
PRIMER_LEFT_9=2377,23
PRIMER_RIGHT_9=2683,23
PRIMER_LEFT_9_TM=59.908
PRIMER_RIGHT_9_TM=59.845
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=5.9000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=307
PRIMER_PAIR_PENALTY_10=0.2512
PRIMER_LEFT_10_PENALTY=0.230707
PRIMER_RIGHT_10_PENALTY=0.020535
PRIMER_LEFT_10_SEQUENCE=CCACCTTTATCCTCTAGCTCTCC
PRIMER_RIGHT_10_SEQUENCE=CACTCAGCTCCATTTCCATAATC
PRIMER_LEFT_10=1733,23
PRIMER_RIGHT_10=1919,23
PRIMER_LEFT_10_TM=59.769
PRIMER_RIGHT_10_TM=59.979
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MER39 a consensus
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=5.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=187
PRIMER_PAIR_PENALTY_11=0.2677
PRIMER_LEFT_11_PENALTY=0.037017
PRIMER_RIGHT_11_PENALTY=0.230707
PRIMER_LEFT_11_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_11_SEQUENCE=GAGAGCTAGAGGATAAAGGTGGG
PRIMER_LEFT_11=1615,23
PRIMER_RIGHT_11=1754,23
PRIMER_LEFT_11_TM=59.963
PRIMER_RIGHT_11_TM=59.769
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=140
PRIMER_PAIR_PENALTY_12=0.2716
PRIMER_LEFT_12_PENALTY=0.261219
PRIMER_RIGHT_12_PENALTY=0.010384
PRIMER_LEFT_12_SEQUENCE=GAGTTGCAGAGGAAGATTATGGA
PRIMER_RIGHT_12_SEQUENCE=TAGAAGCAGTTGGTATGGGAAAA
PRIMER_LEFT_12=1883,23
PRIMER_RIGHT_12=2738,23
PRIMER_LEFT_12_TM=59.739
PRIMER_RIGHT_12_TM=60.010
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=856
PRIMER_PAIR_PENALTY_13=0.2818
PRIMER_LEFT_13_PENALTY=0.261219
PRIMER_RIGHT_13_PENALTY=0.020535
PRIMER_LEFT_13_SEQUENCE=GGAAGCATAGATGGAGACAGAAA
PRIMER_RIGHT_13_SEQUENCE=CACTCAGCTCCATTTCCATAATC
PRIMER_LEFT_13=1514,23
PRIMER_RIGHT_13=1919,23
PRIMER_LEFT_13_TM=59.739
PRIMER_RIGHT_13_TM=59.979
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=5.9000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=406
PRIMER_PAIR_PENALTY_14=0.2982
PRIMER_LEFT_14_PENALTY=0.037017
PRIMER_RIGHT_14_PENALTY=0.261219
PRIMER_LEFT_14_SEQUENCE=CAGGAGTCTGGAGTCAGTTCTGT
PRIMER_RIGHT_14_SEQUENCE=TCCATAATCTTCCTCTGCAACTC
PRIMER_LEFT_14=1615,23
PRIMER_RIGHT_14=1905,23
PRIMER_LEFT_14_TM=59.963
PRIMER_RIGHT_14_TM=59.739
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=291
PRIMER_PAIR_PENALTY_15=0.3056
PRIMER_LEFT_15_PENALTY=0.150712
PRIMER_RIGHT_15_PENALTY=0.154909
PRIMER_LEFT_15_SEQUENCE=CATTCTCAAACAAGAGGACTTGG
PRIMER_RIGHT_15_SEQUENCE=AGGGGCTAGTCCATCTCATAATC
PRIMER_LEFT_15=2375,23
PRIMER_RIGHT_15=2683,23
PRIMER_LEFT_15_TM=60.151
PRIMER_RIGHT_15_TM=59.845
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=5.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=309
PRIMER_PAIR_PENALTY_16=0.3174
PRIMER_LEFT_16_PENALTY=0.315379
PRIMER_RIGHT_16_PENALTY=0.002035
PRIMER_LEFT_16_SEQUENCE=CCATCATCCTTAGGAACTCCTTC
PRIMER_RIGHT_16_SEQUENCE=ACATTAAGCTCCTCTAGGATGGG
PRIMER_LEFT_16=86,23
PRIMER_RIGHT_16=210,23
PRIMER_LEFT_16_TM=60.315
PRIMER_RIGHT_16_TM=59.998
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=8.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=125
PRIMER_PAIR_PENALTY_17=0.3523
PRIMER_LEFT_17_PENALTY=0.091795
PRIMER_RIGHT_17_PENALTY=0.260543
PRIMER_LEFT_17_SEQUENCE=TTCTCAAACAAGAGGACTTGGAG
PRIMER_RIGHT_17_SEQUENCE=AAACAAAGGGGAATGTGAGAACT
PRIMER_LEFT_17=2377,23
PRIMER_RIGHT_17=2716,23
PRIMER_LEFT_17_TM=59.908
PRIMER_RIGHT_17_TM=60.261
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=340
PRIMER_PAIR_PENALTY_18=0.3601
PRIMER_LEFT_18_PENALTY=0.205235
PRIMER_RIGHT_18_PENALTY=0.154909
PRIMER_LEFT_18_SEQUENCE=ACCATATTGACCTGGGGTATAGG
PRIMER_RIGHT_18_SEQUENCE=AGGGGCTAGTCCATCTCATAATC
PRIMER_LEFT_18=2551,23
PRIMER_RIGHT_18=2683,23
PRIMER_LEFT_18_TM=60.205
PRIMER_RIGHT_18_TM=59.845
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.1000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=133
PRIMER_PAIR_PENALTY_19=0.3763
PRIMER_LEFT_19_PENALTY=0.365880
PRIMER_RIGHT_19_PENALTY=0.010384
PRIMER_LEFT_19_SEQUENCE=GAGGACTTGGAGGAAAAGAGCTA
PRIMER_RIGHT_19_SEQUENCE=TAGAAGCAGTTGGTATGGGAAAA
PRIMER_LEFT_19=2388,23
PRIMER_RIGHT_19=2738,23
PRIMER_LEFT_19_TM=60.366
PRIMER_RIGHT_19_TM=60.010
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=351
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