PRIMER_SEQUENCE_ID=NM_004119
SEQUENCE=CGAGGCGGCATCCGAGGGCTGGGCCGGCGCCCTGGGGGACCCCGGGCTCCGGAGGCCATGCCGGCGTTGGCGCGCGACGCGGGCACCGTGCCGCTGCTCGTTGTTTTTTCTGCAATGATATTTGGGACTATTACAAATCAAGATCTGCCTGTGATCAAGTGTGTTTTAATCAATCATAAGAACAATGATTCATCAGTGGGGAAGTCATCATCATATCCCATGGTATCAGAATCCCCGGAAGACCTCGGGTGTGCGTTGAGACCCCAGAGCTCAGGGACAGTGTACGAAGCTGCCGCTGTGGAAGTGGATGTATCTGCTTCCATCACACTGCAAGTGCTGGTCGATGCCCCAGGGAACATTTCCTGTCTCTGGGTCTTTAAGCACAGCTCCCTGAATTGCCAGCCACATTTTGATTTACAAAACAGAGGAGTTGTTTCCATGGTCATTTTGAAAATGACAGAAACCCAAGCTGGAGAATACCTACTTTTTATTCAGAGTGAAGCTACCAATTACACAATATTGTTTACAGTGAGTATAAGAAATACCCTGCTTTACACATTAAGAAGACCTTACTTTAGAAAAATGGAAAACCAGGACGCCCTGGTCTGCATATCTGAGAGCGTTCCAGAGCCGATCGTGGAATGGGTGCTTTGCGATTCACAGGGGGAAAGCTGTAAAGAAGAAAGTCCAGCTGTTGTTAAAAAGGAGGAAAAAGTGCTTCATGAATTATTTGGGACGGACATAAGGTGCTGTGCCAGAAATGAACTGGGCAGGGAATGCACCAGGCTGTTCACAATAGATCTAAATCAAACTCCTCAGACCACATTGCCACAATTATTTCTTAAAGTAGGGGAACCCTTATGGATAAGGTGCAAAGCTGTTCATGTGAACCATGGATTCGGGCTCACCTGGGAATTAGAAAACAAAGCACTCGAGGAGGGCAACTACTTTGAGATGAGTACCTATTCAACAAACAGAACTATGATACGGATTCTGTTTGCTTTTGTATCATCAGTGGCAAGAAACGACACCGGATACTACACTTGTTCCTCTTCAAAGCATCCCAGTCAATCAGCTTTGGTTACCATCGTAGGAAAGGGATTTATAAATGCTACCAATTCAAGTGAAGATTATGAAATTGACCAATATGAAGAGTTTTGTTTTTCTGTCAGGTTTAAAGCCTACCCACAAATCAGATGTACGTGGACCTTCTCTCGAAAATCATTTCCTTGTGAGCAAAAGGGTCTTGATAACGGATACAGCATATCCAAGTTTTGCAATCATAAGCACCAGCCAGGAGAATATATATTCCATGCAGAAAATGATGATGCCCAATTTACCAAAATGTTCACGCTGAATATAAGAAGGAAACCTCAAGTGCTCGCAGAAGCATCGGCAAGTCAGGCGTCCTGTTTCTCGGATGGATACCCATTACCATCTTGGACCTGGAAGAAGTGTTCAGACAAGTCTCCCAACTGCACAGAAGAGATCACAGAAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTGTCGAGCAGTACTCTAAACATGAGTGAAGCCATAAAAGGGTTCCTGGTCAAGTGCTGTGCATACAATTCCCTTGGCACATCTTGTGAGACGATCCTTTTAAACTCTCCAGGCCCCTTCCCTTTCATCCAAGACAACATCTCATTCTATGCAACAATTGGTGTTTGTCTCCTCTTCATTGTCGTTTTAACCCTGCTAATTTGTCACAAGTACAAAAAGCAATTTAGGTATGAAAGCCAGCTACAGATGGTACAGGTGACCGGCTCCTCAGATAATGAGTACTTCTACGTTGATTTCAGAGAATATGAATATGATCTCAAATGGGAGTTTCCAAGAGAAAATTTAGAGTTTGGGAAGGTACTAGGATCAGGTGCTTTTGGAAAAGTGATGAACGCAACAGCTTATGGAATTAGCAAAACAGGAGTCTCAATCCAGGTTGCCGTCAAAATGCTGAAAGAAAAAGCAGACAGCTCTGAAAGAGAGGCACTCATGTCAGAACTCAAGATGATGACCCAGCTGGGAAGCCACGAGAATATTGTGAACCTGCTGGGGGCGTGCACACTGTCAGGACCAATTTACTTGATTTTTGAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGAAAAATTTCACAGGACTTGGACAGAGATTTTCAAGGAACACAATTTCAGTTTTTACCCCACTTTCCAATCACATCCAAATTCCAGCATGCCTGGTTCAAGAGAAGTTCAGATACACCCGGACTCGGATCAAATCTCAGGGCTTCATGGGAATTCATTTCACTCTGAAGATGAAATTGAATATGAAAACCAAAAAAGGCTGGAAGAAGAGGAGGACTTGAATGTGCTTACATTTGAAGATCTTCTTTGCTTTGCATATCAAGTTGCCAAAGGAATGGAATTTCTGGAATTTAAGTCGTGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTGGCTCGAGATATCATGAGTGATTCCAACTATGTTGTCAGGGGCAATGCCCGTCTGCCTGTAAAATGGATGGCCCCCGAAAGCCTGTTTGAAGGCATCTACACCATTAAGAGTGATGTCTGGTCATATGGAATATTACTGTGGGAAATCTTCTCACTTGGTGTGAATCCTTACCCTGGCATTCCGGTTGATGCTAACTTCTACAAACTGATTCAAAATGGATTTAAAATGGATCAGCCATTTTATGCTACAGAAGAAATATACATTATAATGCAATCCTGCTGGGCTTTTGACTCAAGGAAACGGCCATCCTTCCCTAATTTGACTTCGTTTTTAGGATGTCAGCTGGCAGATGCAGAAGAAGCGATGTATCAGAATGTGGATGGCCGTGTTTCGGAATGTCCTCACACCTACCAAAACAGGCGACCTTTCAGCAGAGAGATGGATTTGGGGCTACTCTCTCCGCAGGCTCAGGTCGAAGATTCGTAGAGGAACAATTTAGTTTTAAGGACTTCATCCCTCCACCTATCCCTAACAGGCTGTAGATTACCAAAACAAGATTAATTTCATCACTAAAAGAAAATCTATTATCAACTGCTGCTTCACCAGACTTTTCTCTAGAAGCCGTCTGCGTTTACTCTTGTTTTCAAAGGGACTTTTGTAAAATCAAATCATCCTGTCACAAGGCAGGAGGAGCTGATAATGAACTTTATTGGAGCATTGATCTGCATCCAAGGCCTTCTCAGGCCGGCTTGAGTGAATTGTGTACCTGAAGTACAGTATATTCTTGTAAATACATAAAACAAAAGCATTTTGCTAAGGAGAAGCTAATATGATTTTTTAAGTCTATGTTTTAAAATAATATGTAAATTTTTCAGCTATTTAGTGATATATTTTATGGGTGGGAATAAAATTTCTACTACAG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 40147, GC content failed 1240, low tm 20643, high tm 9140, high any compl 2, high end compl 25, high repeat similarity 41, long poly-x seq 52, ok 9004
PRIMER_RIGHT_EXPLAIN=considered 40089, GC content failed 1022, low tm 21052, high tm 8847, high any compl 1, high end compl 14, high repeat similarity 12, long poly-x seq 58, ok 9083
PRIMER_PAIR_EXPLAIN=considered 563, unacceptable product size 504, high end compl 4, ok 55
PRIMER_PAIR_PENALTY=0.0093
PRIMER_LEFT_PENALTY=0.006255
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=GTCAGAACTCAAGATGATGACCC
PRIMER_RIGHT_SEQUENCE=CTGTAGCATAAAATGGCTGATCC
PRIMER_LEFT=2033,23
PRIMER_RIGHT=2802,23
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=770
PRIMER_PAIR_PENALTY_1=0.0238
PRIMER_LEFT_1_PENALTY=0.020735
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=GAAGCCACGAGAATATTGTGAAC
PRIMER_RIGHT_1_SEQUENCE=CTGTAGCATAAAATGGCTGATCC
PRIMER_LEFT_1=2062,23
PRIMER_RIGHT_1=2802,23
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, reverse MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=741
PRIMER_PAIR_PENALTY_2=0.0350
PRIMER_LEFT_2_PENALTY=0.018344
PRIMER_RIGHT_2_PENALTY=0.016658
PRIMER_LEFT_2_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_2_SEQUENCE=CAAAAGCAAACAGAATCCGTATC
PRIMER_LEFT_2=132,23
PRIMER_RIGHT_2=1005,23
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.017
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=5.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=874
PRIMER_PAIR_PENALTY_3=0.0367
PRIMER_LEFT_3_PENALTY=0.018344
PRIMER_RIGHT_3_PENALTY=0.018344
PRIMER_LEFT_3_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_3_SEQUENCE=GAAGCAGATACATCCACTTCCAC
PRIMER_LEFT_3=132,23
PRIMER_RIGHT_3=319,23
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, MER4C a consensus
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.0461
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=AAAGCAGACAGCTCTGAAAGAGA
PRIMER_RIGHT_4_SEQUENCE=CTGTAGCATAAAATGGCTGATCC
PRIMER_LEFT_4=2001,23
PRIMER_RIGHT_4=2802,23
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=802
PRIMER_PAIR_PENALTY_5=0.0475
PRIMER_LEFT_5_PENALTY=0.030877
PRIMER_RIGHT_5_PENALTY=0.016658
PRIMER_LEFT_5_SEQUENCE=CCCAAGCTGGAGAATACCTACTT
PRIMER_RIGHT_5_SEQUENCE=CAAAAGCAAACAGAATCCGTATC
PRIMER_LEFT_5=463,23
PRIMER_RIGHT_5=1005,23
PRIMER_LEFT_5_TM=60.031
PRIMER_RIGHT_5_TM=60.017
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=5.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=543
PRIMER_PAIR_PENALTY_6=0.0776
PRIMER_LEFT_6_PENALTY=0.018344
PRIMER_RIGHT_6_PENALTY=0.059210
PRIMER_LEFT_6_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_6_SEQUENCE=TGTAGTATCCGGTGTCGTTTCTT
PRIMER_LEFT_6=132,23
PRIMER_RIGHT_6=1041,23
PRIMER_LEFT_6_TM=60.018
PRIMER_RIGHT_6_TM=59.941
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.3000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=910
PRIMER_PAIR_PENALTY_7=0.0776
PRIMER_LEFT_7_PENALTY=0.018344
PRIMER_RIGHT_7_PENALTY=0.059210
PRIMER_LEFT_7_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_7_SEQUENCE=GTAGTATCCGGTGTCGTTTCTTG
PRIMER_LEFT_7=132,23
PRIMER_RIGHT_7=1040,23
PRIMER_LEFT_7_TM=60.018
PRIMER_RIGHT_7_TM=59.941
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=909
PRIMER_PAIR_PENALTY_8=0.0777
PRIMER_LEFT_8_PENALTY=0.061028
PRIMER_RIGHT_8_PENALTY=0.016658
PRIMER_LEFT_8_SEQUENCE=ACAAAACAGAGGAGTTGTTTCCA
PRIMER_RIGHT_8_SEQUENCE=CAAAAGCAAACAGAATCCGTATC
PRIMER_LEFT_8=416,23
PRIMER_RIGHT_8=1005,23
PRIMER_LEFT_8_TM=60.061
PRIMER_RIGHT_8_TM=60.017
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=5.3000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=590
PRIMER_PAIR_PENALTY_9=0.0794
PRIMER_LEFT_9_PENALTY=0.018344
PRIMER_RIGHT_9_PENALTY=0.061028
PRIMER_LEFT_9_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_9_SEQUENCE=TGGAAACAACTCCTCTGTTTTGT
PRIMER_LEFT_9=132,23
PRIMER_RIGHT_9=438,23
PRIMER_LEFT_9_TM=60.018
PRIMER_RIGHT_9_TM=60.061
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.3000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=307
PRIMER_PAIR_PENALTY_10=0.0804
PRIMER_LEFT_10_PENALTY=0.018344
PRIMER_RIGHT_10_PENALTY=0.062009
PRIMER_LEFT_10_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_10_SEQUENCE=CTCCTCGAGTGCTTTGTTTTCTA
PRIMER_LEFT_10=132,23
PRIMER_RIGHT_10=938,23
PRIMER_LEFT_10_TM=60.018
PRIMER_RIGHT_10_TM=60.062
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, Tigger1 Autonomous DNA transposon
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=6.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=807
PRIMER_PAIR_PENALTY_11=0.0901
PRIMER_LEFT_11_PENALTY=0.030877
PRIMER_RIGHT_11_PENALTY=0.059210
PRIMER_LEFT_11_SEQUENCE=CCCAAGCTGGAGAATACCTACTT
PRIMER_RIGHT_11_SEQUENCE=GTAGTATCCGGTGTCGTTTCTTG
PRIMER_LEFT_11=463,23
PRIMER_RIGHT_11=1040,23
PRIMER_LEFT_11_TM=60.031
PRIMER_RIGHT_11_TM=59.941
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=578
PRIMER_PAIR_PENALTY_12=0.0901
PRIMER_LEFT_12_PENALTY=0.030877
PRIMER_RIGHT_12_PENALTY=0.059210
PRIMER_LEFT_12_SEQUENCE=CCCAAGCTGGAGAATACCTACTT
PRIMER_RIGHT_12_SEQUENCE=TGTAGTATCCGGTGTCGTTTCTT
PRIMER_LEFT_12=463,23
PRIMER_RIGHT_12=1041,23
PRIMER_LEFT_12_TM=60.031
PRIMER_RIGHT_12_TM=59.941
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=579
PRIMER_PAIR_PENALTY_13=0.0929
PRIMER_LEFT_13_PENALTY=0.030877
PRIMER_RIGHT_13_PENALTY=0.062009
PRIMER_LEFT_13_SEQUENCE=CCCAAGCTGGAGAATACCTACTT
PRIMER_RIGHT_13_SEQUENCE=CTCCTCGAGTGCTTTGTTTTCTA
PRIMER_LEFT_13=463,23
PRIMER_RIGHT_13=938,23
PRIMER_LEFT_13_TM=60.031
PRIMER_RIGHT_13_TM=60.062
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=6.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=476
PRIMER_PAIR_PENALTY_14=0.0936
PRIMER_LEFT_14_PENALTY=0.018344
PRIMER_RIGHT_14_PENALTY=0.075241
PRIMER_LEFT_14_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_14_SEQUENCE=ACATGAACAGCTTTGCACCTTAT
PRIMER_LEFT_14=132,23
PRIMER_RIGHT_14=886,23
PRIMER_LEFT_14_TM=60.018
PRIMER_RIGHT_14_TM=60.075
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_14_END_STABILITY=6.3000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=755
PRIMER_PAIR_PENALTY_15=0.1029
PRIMER_LEFT_15_PENALTY=0.086226
PRIMER_RIGHT_15_PENALTY=0.016658
PRIMER_LEFT_15_SEQUENCE=AACCCAAGCTGGAGAATACCTAC
PRIMER_RIGHT_15_SEQUENCE=CAAAAGCAAACAGAATCCGTATC
PRIMER_LEFT_15=461,23
PRIMER_RIGHT_15=1005,23
PRIMER_LEFT_15_TM=59.914
PRIMER_RIGHT_15_TM=60.017
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=5.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=545
PRIMER_PAIR_PENALTY_16=0.1035
PRIMER_LEFT_16_PENALTY=0.100425
PRIMER_RIGHT_16_PENALTY=0.003045
PRIMER_LEFT_16_SEQUENCE=AAAAATTTCACAGGACTTGGACA
PRIMER_RIGHT_16_SEQUENCE=CTGTAGCATAAAATGGCTGATCC
PRIMER_LEFT_16=2179,23
PRIMER_RIGHT_16=2802,23
PRIMER_LEFT_16_TM=59.900
PRIMER_RIGHT_16_TM=60.003
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=7.8000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=624
PRIMER_PAIR_PENALTY_17=0.1046
PRIMER_LEFT_17_PENALTY=0.018344
PRIMER_RIGHT_17_PENALTY=0.086226
PRIMER_LEFT_17_SEQUENCE=ACAAATCAAGATCTGCCTGTGAT
PRIMER_RIGHT_17_SEQUENCE=GTAGGTATTCTCCAGCTTGGGTT
PRIMER_LEFT_17=132,23
PRIMER_RIGHT_17=483,23
PRIMER_LEFT_17_TM=60.018
PRIMER_RIGHT_17_TM=59.914
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, Tigger1 Autonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.3000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=352
PRIMER_PAIR_PENALTY_18=0.1061
PRIMER_LEFT_18_PENALTY=0.030877
PRIMER_RIGHT_18_PENALTY=0.075241
PRIMER_LEFT_18_SEQUENCE=CCCAAGCTGGAGAATACCTACTT
PRIMER_RIGHT_18_SEQUENCE=ACATGAACAGCTTTGCACCTTAT
PRIMER_LEFT_18=463,23
PRIMER_RIGHT_18=886,23
PRIMER_LEFT_18_TM=60.031
PRIMER_RIGHT_18_TM=60.075
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=424
PRIMER_PAIR_PENALTY_19=0.1092
PRIMER_LEFT_19_PENALTY=0.006255
PRIMER_RIGHT_19_PENALTY=0.102951
PRIMER_LEFT_19_SEQUENCE=GTCAGAACTCAAGATGATGACCC
PRIMER_RIGHT_19_SEQUENCE=GATACATCGCTTCTTCTGCATCT
PRIMER_LEFT_19=2033,23
PRIMER_RIGHT_19=2922,23
PRIMER_LEFT_19_TM=59.994
PRIMER_RIGHT_19_TM=59.897
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.1000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=890
=