PRIMER_SEQUENCE_ID=NM_004304
SEQUENCE=AAGCGGGGGCGGCAGCGGTGGTAGCAGCTGGTACCTCCCGCCGCCTCTGTTCGGAGGGTCGCGGGGCACCGAGGTGCTTTCCGGCCGCCCTCTGGTCGGCCACCCAAAGCCGCGGGCGCTGATGATGGGTGAGGAGGGGGCGGCAAGATTTCGGGCGCCCCTGCCCTGAACGCCCTCAGCTGCTGCCGCCGGGGCCGCTCCAGTGCCTGCGAACTCTGAGGAGCCGAGGCGCCGGTGAGAGCAAGGACGCTGCAAACTTGCGCAGCGCGGGGGCTGGGATTCACGCCCAGAAGTTCAGCAGGCAGACAGTCCGAAGCCTTCCCGCAGCGGAGAGATAGCTTGAGGGTGCGCAAGACGGCAGCCTCCGCCCTCGGTTCCCGCCCAGACCGGGCAGAAGAGCTTGGAGGAGCCACAAGGAACGCAAAAGGCGGCCAGGACAGCGTGCAGCAGCTGGGAGCCGCCGTTCTCAGCCTTAAAAGTTGCAGAGATTGGAGGCTGCCCCGAGAGGGGACAGACCCCAGCTCCGACTGCGGGGGGCAGGAGAGGACGGTACCCAACTGCCACCTCCCTTCAACCATAGTAGTTCCTCTGTACCGAGCGCAGCGAGCTACAGACGGGGGCGCGGCACTCGGCGCGGAGAGCGGGAGGCTCAAGGTCCCAGCCAGTGAGCCCAGTGTGCTTGAGTGTCTCTGGACTCGCCCCTGAGCTTCCAGGTCTGTTTCATTTAGACTCCTGCTCGCCTCCGTGCAGTTGGGGGAAAGCAAGAGACTTGCGCGCACGCACAGTCCTCTGGAGATCAGGTGGAAGGAGCCGCTGGGTACCAAGGACTGTTCAGAGCCTCTTCCCATCTCGGGGAGAGCGAAGGGTGAGGCTGGGCCCGGAGAGCAGTGTAAACGGCCTCCTCCGGCGGGATGGGAGCCATCGGGCTCCTGTGGCTGCTGCCGCTGCTGCTTTCCACGGCAGCTGTGGGCTCCGGGATGGGGACCGGCCAGCGCGCGGGCTCCCCAGCTGCGGGGCCGCCGCTGCAGCCCCGGGAGCCACTCAGCTACTCGCGCCTGCAGAGGAAGAGTCTGGCAGTTGACTTCGTGGTGCCCTCGCTCTTCCGTGTCTACGCCCGGGACCTACTGCTGCCACCATCCTCCTCGGAGCTGAAGGCTGGCAGGCCCGAGGCCCGCGGCTCGCTAGCTCTGGACTGCGCCCCGCTGCTCAGGTTGCTGGGGCCGGCGCCGGGGGTCTCCTGGACCGCCGGTTCACCAGCCCCGGCAGAGGCCCGGACGCTGTCCAGGGTGCTGAAGGGCGGCTCCGTGCGCAAGCTCCGGCGTGCCAAGCAGTTGGTGCTGGAGCTGGGCGAGGAGGCGATCTTGGAGGGTTGCGTCGGGCCCCCCGGGGAGGCGGCTGTGGGGCTGCTCCAGTTCAATCTCAGCGAGCTGTTCAGTTGGTGGATTCGCCAAGGCGAAGGGCGACTGAGGATCCGCCTGATGCCCGAGAAGAAGGCGTCGGAAGTGGGCAGAGAGGGAAGGCTGTCCGCGGCAATTCGCGCCTCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACTGGTCATAGCTCCTTGGAATCACCAACAAACATGCCTTCTCCTTCTCCTGATTATTTTACATGGAATCTCACCTGGATAATGAAAGACTCCTTCCCTTTCCTGTCTCATCGCAGCCGATATGGTCTGGAGTGCAGCTTTGACTTCCCCTGTGAGCTGGAGTATTCCCCTCCACTGCATGACCTCAGGAACCAGAGCTGGTCCTGGCGCCGCATCCCCTCCGAGGAGGCCTCCCAGATGGACTTGCTGGATGGGCCTGGGGCAGAGCGTTCTAAGGAGATGCCCAGAGGCTCCTTTCTCCTTCTCAACACCTCAGCTGACTCCAAGCACACCATCCTGAGTCCGTGGATGAGGAGCAGCAGTGAGCACTGCACACTGGCCGTCTCGGTGCACAGGCACCTGCAGCCCTCTGGAAGGTACATTGCCCAGCTGCTGCCCCACAACGAGGCTGCAAGAGAGATCCTCCTGATGCCCACTCCAGGGAAGCATGGTTGGACAGTGCTCCAGGGAAGAATCGGGCGTCCAGACAACCCATTTCGAGTGGCCCTGGAATACATCTCCAGTGGAAACCGCAGCTTGTCTGCAGTGGACTTCTTTGCCCTGAAGAACTGCAGTGAAGGAACATCCCCAGGCTCCAAGATGGCCCTGCAGAGCTCCTTCACTTGTTGGAATGGGACAGTCCTCCAGCTTGGGCAGGCCTGTGACTTCCACCAGGACTGTGCCCAGGGAGAAGATGAGAGCCAGATGTGCCGGAAACTGCCTGTGGGTTTTTACTGCAACTTTGAAGATGGCTTCTGTGGCTGGACCCAAGGCACACTGTCACCCCACACTCCTCAGTGGCAGGTCAGGACCCTAAAGGATGCCCGGTTCCAGGACCACCAAGACCATGCTCTATTGCTCAGTACCACTGATGTCCCCGCTTCTGAAAGTGCTACAGTGACCAGTGCTACGTTTCCTGCACCGATCAAGAGCTCTCCATGTGAGCTCCGAATGTCCTGGCTCATTCGTGGAGTCTTGAGGGGAAACGTGTCCTTGGTGCTAGTGGAGAACAAAACCGGGAAGGAGCAAGGCAGGATGGTCTGGCATGTCGCCGCCTATGAAGGCTTGAGCCTGTGGCAGTGGATGGTGTTGCCTCTCCTCGATGTGTCTGACAGGTTCTGGCTGCAGATGGTCGCATGGTGGGGACAAGGATCCAGAGCCATCGTGGCTTTTGACAATATCTCCATCAGCCTGGACTGCTACCTCACCATTAGCGGAGAGGACAAGATCCTGCAGAATACAGCACCCAAATCAAGAAACCTGTTTGAGAGAAACCCAAACAAGGAGCTGAAACCCGGGGAAAATTCACCAAGACAGACCCCCATCTTTGACCCTACAGTTCATTGGCTGTTCACCACATGTGGGGCCAGCGGGCCCCATGGCCCCACCCAGGCACAGTGCAACAACGCCTACCAGAACTCCAACCTGAGCGTGGAGGTGGGGAGCGAGGGCCCCCTGAAAGGCATCCAGATCTGGAAGGTGCCAGCCACCGACACCTACAGCATCTCGGGCTACGGAGCTGCTGGCGGGAAAGGCGGGAAGAACACCATGATGCGGTCCCACGGCGTGTCTGTGCTGGGCATCTTCAACCTGGAGAAGGATGACATGCTGTACATCCTGGTTGGGCAGCAGGGAGAGGACGCCTGCCCCAGTACAAACCAGTTAATCCAGAAAGTCTGCATTGGAGAGAACAATGTGATAGAAGAAGAAATCCGTGTGAACAGAAGCGTGCATGAGTGGGCAGGAGGCGGAGGAGGAGGGGGTGGAGCCACCTACGTATTTAAGATGAAGGATGGAGTGCCGGTGCCCCTGATCATTGCAGCCGGAGGTGGTGGCAGGGCCTACGGGGCCAAGACAGACACGTTCCACCCAGAGAGACTGGAGAATAACTCCTCGGTTCTAGGGCTAAACGGCAATTCCGGAGCCGCAGGTGGTGGAGGTGGCTGGAATGATAACACTTCCTTGCTCTGGGCCGGAAAATCTTTGCAGGAGGGTGCCACCGGAGGACATTCCTGCCCCCAGGCCATGAAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCTCAGGTGGAGGAGGCGGAGGATATATAGGCGGCAATGCAGCCTCAAACAATGACCCCGAAATGGATGGGGAAGATGGGGTTTCCTTCATCAGTCCACTGGGCATCCTGTACACCCCAGCTTTAAAAGTGATGGAAGGCCACGGGGAAGTGAATATTAAGCATTATCTAAACTGCAGTCACTGTGAGGTAGACGAATGTCACATGGACCCTGAAAGCCACAAGGTCATCTGCTTCTGTGACCACGGGACGGTGCTGGCTGAGGATGGCGTCTCCTGCATTGTGTCACCCACCCCGGAGCCACACCTGCCACTCTCGCTGATCCTCTCTGTGGTGACCTCTGCCCTCGTGGCCGCCCTGGTCCTGGCTTTCTCCGGCATCATGATTGTGTACCGCCGGAAGCACCAGGAGCTGCAAGCCATGCAGATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATGACCGACTACAACCCCAACTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAGGTGCCGCGGAAAAACATCACCCTCATTCGGGGTCTGGGCCATGGCGCCTTTGGGGAGGTGTATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTGAAGACGCTGCCTGAAGTGTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATCATCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTGCAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGAGAGACCCGCCCTCGCCCGAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCTCGGGACATTGCCTGTGGCTGTCAGTATTTGGAGGAAAACCACTTCATCCACCGAGACATTGCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCGAGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATGCTGCCAGTTAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGAATATTCACTTCTAAAACAGACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCATACCCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCACCCAAGAACTGCCCTGGGCCTGTATACCGGATAATGACTCAGTGCTGGCAACATCAGCCTGAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGACCCGGATGTAATCAACACCGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAAGTGCCTGTGAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTCCTGGTCTCTCAACAGGCAAAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCTGGCAAGGCTGCAAAGAAACCCACAGCTGCAGAGGTCTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGCACAGGGTCCACGGATCCAGAAATAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGGTTTACAGAGAAACCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGAGAGGGGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGTTGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCTCTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGAGGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCCTTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAATCAATGGCTCCTTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCACCAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGAAAATATCATAAAAATGAGTGATAAATACAAGGCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTTGTACTCTGTTCGAGTCCCTAGAGGGCCGGCCGTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGCACAAGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGGTTTACAGAGAAACCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGACAGGGGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGTTGCAACTGGGAGACTTCCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCTCTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGCTTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGAGGATACCATTCTGAAAAGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCCTTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAATGGCTCCTTCACAAACCAGAGACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCACCAAAAAAGCTGTATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAGAAAATATCATAAAAATGAGTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTATGCATGTTTGTTGTATACTTCCTTATGCTTCTTTTAAATTGTGTGTGCTCTGCTTCAATGTAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTGTTGATGTGGACATGAGCCATTTGAGGGGAGAGGGAACGGAAATAAAGGAGTTATTTGTAATGACTAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 81860, GC content failed 2778, low tm 22369, high tm 40620, high any compl 10, high end compl 12, high repeat similarity 25, long poly-x seq 109, ok 15937
PRIMER_RIGHT_EXPLAIN=considered 81863, GC content failed 2697, low tm 22767, high tm 40191, high any compl 3, high end compl 6, high repeat similarity 11, long poly-x seq 110, ok 16078
PRIMER_PAIR_EXPLAIN=considered 916, unacceptable product size 856, high end compl 6, ok 54
PRIMER_PAIR_PENALTY=0.0348
PRIMER_LEFT_PENALTY=0.020535
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=TTTGACAATATCTCCATCAGCCT
PRIMER_RIGHT_SEQUENCE=CAAGGAAGTGTTATCATTCCAGC
PRIMER_LEFT=2783,23
PRIMER_RIGHT=3577,23
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=795
PRIMER_PAIR_PENALTY_1=0.0359
PRIMER_LEFT_1_PENALTY=0.021648
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CCAAATCAAGAAACCTGTTTGAG
PRIMER_RIGHT_1_SEQUENCE=CAAGGAAGTGTTATCATTCCAGC
PRIMER_LEFT_1=2859,23
PRIMER_RIGHT_1=3577,23
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=719
PRIMER_PAIR_PENALTY_2=0.0432
PRIMER_LEFT_2_PENALTY=0.028939
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=AGAAACCTGTTTGAGAGAAACCC
PRIMER_RIGHT_2_SEQUENCE=CAAGGAAGTGTTATCATTCCAGC
PRIMER_LEFT_2=2867,23
PRIMER_RIGHT_2=3577,23
PRIMER_LEFT_2_TM=60.029
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=711
PRIMER_PAIR_PENALTY_3=0.0443
PRIMER_LEFT_3_PENALTY=0.030116
PRIMER_RIGHT_3_PENALTY=0.014220
PRIMER_LEFT_3_SEQUENCE=TGGAGCCACCTACGTATTTAAGA
PRIMER_RIGHT_3_SEQUENCE=CAAGGAAGTGTTATCATTCCAGC
PRIMER_LEFT_3=3376,23
PRIMER_RIGHT_3=3577,23
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.0942
PRIMER_LEFT_4_PENALTY=0.088643
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TGGTCATTACGAGGATACCATTC
PRIMER_RIGHT_4_SEQUENCE=CTACATTGAAGCAGAGCACACAC
PRIMER_LEFT_4=6476,23
PRIMER_RIGHT_4=6862,23
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=387
PRIMER_PAIR_PENALTY_5=0.1025
PRIMER_LEFT_5_PENALTY=0.080854
PRIMER_RIGHT_5_PENALTY=0.021648
PRIMER_LEFT_5_SEQUENCE=GCGAGCTACTATAGAAAGGGAGG
PRIMER_RIGHT_5_SEQUENCE=TCTTTTTGGTGGGTTTCTCTGTA
PRIMER_LEFT_5=4748,23
PRIMER_RIGHT_5=5465,23
PRIMER_LEFT_5_TM=59.919
PRIMER_RIGHT_5_TM=60.022
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=718
PRIMER_PAIR_PENALTY_6=0.1025
PRIMER_LEFT_6_PENALTY=0.080854
PRIMER_RIGHT_6_PENALTY=0.021648
PRIMER_LEFT_6_SEQUENCE=GGCGAGCTACTATAGAAAGGGAG
PRIMER_RIGHT_6_SEQUENCE=TCTTTTTGGTGGGTTTCTCTGTA
PRIMER_LEFT_6=4747,23
PRIMER_RIGHT_6=5465,23
PRIMER_LEFT_6_TM=59.919
PRIMER_RIGHT_6_TM=60.022
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=719
PRIMER_PAIR_PENALTY_7=0.1025
PRIMER_LEFT_7_PENALTY=0.080854
PRIMER_RIGHT_7_PENALTY=0.021648
PRIMER_LEFT_7_SEQUENCE=CGAGCTACTATAGAAAGGGAGGC
PRIMER_RIGHT_7_SEQUENCE=TCTTTTTGGTGGGTTTCTCTGTA
PRIMER_LEFT_7=4749,23
PRIMER_RIGHT_7=5465,23
PRIMER_LEFT_7_TM=59.919
PRIMER_RIGHT_7_TM=60.022
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.4000
PRIMER_RIGHT_7_END_STABILITY=5.7000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=717
PRIMER_PAIR_PENALTY_8=0.1096
PRIMER_LEFT_8_PENALTY=0.014220
PRIMER_RIGHT_8_PENALTY=0.095348
PRIMER_LEFT_8_SEQUENCE=TGGAATGATAACACTTCCTTGCT
PRIMER_RIGHT_8_SEQUENCE=CAAAGCAGTAGTTGGGGTTGTAG
PRIMER_LEFT_8=3557,23
PRIMER_RIGHT_8=4205,23
PRIMER_LEFT_8_TM=60.014
PRIMER_RIGHT_8_TM=60.095
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse MER11A a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, reverse MER11B a consensus
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=649
PRIMER_PAIR_PENALTY_9=0.1103
PRIMER_LEFT_9_PENALTY=0.088643
PRIMER_RIGHT_9_PENALTY=0.021648
PRIMER_LEFT_9_SEQUENCE=TGGTCATTACGAGGATACCATTC
PRIMER_RIGHT_9_SEQUENCE=TCTTTTTGGTGGGTTTCTCTGTA
PRIMER_LEFT_9=5713,23
PRIMER_RIGHT_9=6228,23
PRIMER_LEFT_9_TM=60.089
PRIMER_RIGHT_9_TM=60.022
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.9000
PRIMER_RIGHT_9_END_STABILITY=5.7000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=516
PRIMER_PAIR_PENALTY_10=0.1106
PRIMER_LEFT_10_PENALTY=0.015214
PRIMER_RIGHT_10_PENALTY=0.095348
PRIMER_LEFT_10_SEQUENCE=ACTGGAGAATAACTCCTCGGTTC
PRIMER_RIGHT_10_SEQUENCE=CAAAGCAGTAGTTGGGGTTGTAG
PRIMER_LEFT_10=3490,23
PRIMER_RIGHT_10=4205,23
PRIMER_LEFT_10_TM=60.015
PRIMER_RIGHT_10_TM=60.095
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=5.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=716
PRIMER_PAIR_PENALTY_11=0.1220
PRIMER_LEFT_11_PENALTY=0.014220
PRIMER_RIGHT_11_PENALTY=0.107775
PRIMER_LEFT_11_SEQUENCE=TGGAATGATAACACTTCCTTGCT
PRIMER_RIGHT_11_SEQUENCE=ATGCTTAATATTCACTTCCCCGT
PRIMER_LEFT_11=3557,23
PRIMER_RIGHT_11=3859,23
PRIMER_LEFT_11_TM=60.014
PRIMER_RIGHT_11_TM=60.108
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse MER11A a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_11_END_STABILITY=8.5000
PRIMER_RIGHT_11_END_STABILITY=11.1000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=303
PRIMER_PAIR_PENALTY_12=0.1220
PRIMER_LEFT_12_PENALTY=0.014220
PRIMER_RIGHT_12_PENALTY=0.107775
PRIMER_LEFT_12_SEQUENCE=CTGGAATGATAACACTTCCTTGC
PRIMER_RIGHT_12_SEQUENCE=ATGCTTAATATTCACTTCCCCGT
PRIMER_LEFT_12=3556,23
PRIMER_RIGHT_12=3859,23
PRIMER_LEFT_12_TM=60.014
PRIMER_RIGHT_12_TM=60.108
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=10.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=11.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=304
PRIMER_PAIR_PENALTY_13=0.1230
PRIMER_LEFT_13_PENALTY=0.015214
PRIMER_RIGHT_13_PENALTY=0.107775
PRIMER_LEFT_13_SEQUENCE=ACTGGAGAATAACTCCTCGGTTC
PRIMER_RIGHT_13_SEQUENCE=ATGCTTAATATTCACTTCCCCGT
PRIMER_LEFT_13=3490,23
PRIMER_RIGHT_13=3859,23
PRIMER_LEFT_13_TM=60.015
PRIMER_RIGHT_13_TM=60.108
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, reverse LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_LEFT_13_END_STABILITY=7.9000
PRIMER_RIGHT_13_END_STABILITY=11.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=370
PRIMER_PAIR_PENALTY_14=0.1255
PRIMER_LEFT_14_PENALTY=0.030116
PRIMER_RIGHT_14_PENALTY=0.095348
PRIMER_LEFT_14_SEQUENCE=TGGAGCCACCTACGTATTTAAGA
PRIMER_RIGHT_14_SEQUENCE=CAAAGCAGTAGTTGGGGTTGTAG
PRIMER_LEFT_14=3376,23
PRIMER_RIGHT_14=4205,23
PRIMER_LEFT_14_TM=60.030
PRIMER_RIGHT_14_TM=60.095
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.0000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=830
PRIMER_PAIR_PENALTY_15=0.1367
PRIMER_LEFT_15_PENALTY=0.028939
PRIMER_RIGHT_15_PENALTY=0.107775
PRIMER_LEFT_15_SEQUENCE=AGAAACCTGTTTGAGAGAAACCC
PRIMER_RIGHT_15_SEQUENCE=ATGCTTAATATTCACTTCCCCGT
PRIMER_LEFT_15=2867,23
PRIMER_RIGHT_15=3859,23
PRIMER_LEFT_15_TM=60.029
PRIMER_RIGHT_15_TM=60.108
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=11.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=993
PRIMER_PAIR_PENALTY_16=0.1379
PRIMER_LEFT_16_PENALTY=0.030116
PRIMER_RIGHT_16_PENALTY=0.107775
PRIMER_LEFT_16_SEQUENCE=TGGAGCCACCTACGTATTTAAGA
PRIMER_RIGHT_16_SEQUENCE=ATGCTTAATATTCACTTCCCCGT
PRIMER_LEFT_16=3376,23
PRIMER_RIGHT_16=3859,23
PRIMER_LEFT_16_TM=60.030
PRIMER_RIGHT_16_TM=60.108
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_16_END_STABILITY=6.0000
PRIMER_RIGHT_16_END_STABILITY=11.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=484
PRIMER_PAIR_PENALTY_17=0.1466
PRIMER_LEFT_17_PENALTY=0.141011
PRIMER_RIGHT_17_PENALTY=0.005574
PRIMER_LEFT_17_SEQUENCE=AAAAGCAAGAATAGCATGAACCA
PRIMER_RIGHT_17_SEQUENCE=CTACATTGAAGCAGAGCACACAC
PRIMER_LEFT_17=6501,23
PRIMER_RIGHT_17=6862,23
PRIMER_LEFT_17_TM=60.141
PRIMER_RIGHT_17_TM=59.994
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=362
PRIMER_PAIR_PENALTY_18=0.1552
PRIMER_LEFT_18_PENALTY=0.141011
PRIMER_RIGHT_18_PENALTY=0.014220
PRIMER_LEFT_18_SEQUENCE=AAAAGCAAGAATAGCATGAACCA
PRIMER_RIGHT_18_SEQUENCE=CAAGGCAAGGAAACATCTATGAC
PRIMER_LEFT_18=6501,23
PRIMER_RIGHT_18=6919,23
PRIMER_LEFT_18_TM=60.141
PRIMER_RIGHT_18_TM=60.014
PRIMER_LEFT_18_GC_PERCENT=34.783
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=419
PRIMER_PAIR_PENALTY_19=0.1552
PRIMER_LEFT_19_PENALTY=0.141011
PRIMER_RIGHT_19_PENALTY=0.014220
PRIMER_LEFT_19_SEQUENCE=AAAAGCAAGAATAGCATGAACCA
PRIMER_RIGHT_19_SEQUENCE=ACAAGGCAAGGAAACATCTATGA
PRIMER_LEFT_19=6501,23
PRIMER_RIGHT_19=6920,23
PRIMER_LEFT_19_TM=60.141
PRIMER_RIGHT_19_TM=60.014
PRIMER_LEFT_19_GC_PERCENT=34.783
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=420
=