PRIMER_SEQUENCE_ID=NM_004431
SEQUENCE=CGGAAGTTGCGCGCAGGCCGGCGGGCGGGAGCGGACACCGAGGCCGGCGTGCAGGCGTGCGGGTGTGCGGGAGCCGGGCTCGGGGGGATCGGACCGAGAGCGAGAAGCGCGGCATGGAGCTCCAGGCAGCCCGCGCCTGCTTCGCCCTGCTGTGGGGCTGTGCGCTGGCCGCGGCCGCGGCGGCGCAGGGCAAGGAAGTGGTACTGCTGGACTTTGCTGCAGCTGGAGGGGAGCTCGGCTGGCTCACACACCCGTATGGCAAAGGGTGGGACCTGATGCAGAACATCATGAATGACATGCCGATCTACATGTACTCCGTGTGCAACGTGATGTCTGGCGACCAGGACAACTGGCTCCGCACCAACTGGGTGTACCGAGGAGAGGCTGAGCGTAACAACTTTGAGCTCAACTTTACTGTACGTGACTGCAACAGCTTCCCTGGTGGCGCCAGCTCCTGCAAGGAGACTTTCAACCTCTACTATGCCGAGTCGGACCTGGACTACGGCACCAACTTCCAGAAGCGCCTGTTCACCAAGATTGACACCATTGCGCCCGATGAGATCACCGTCAGCAGCGACTTCGAGGCACGCCACGTGAAGCTGAACGTGGAGGAGCGCTCCGTGGGGCCGCTCACCCGCAAAGGCTTCTACCTGGCCTTCCAGGATATCGGTGCCTGTGTGGCGCTGCTCTCCGTCCGTGTCTACTACAAGAAGTGCCCCGAGCTGCTGCAGGGCCTGGCCCACTTCCCTGAGACCATCGCCGGCTCTGATGCACCTTCCCTGGCCACTGTGGCCGGCACCTGTGTGGACCATGCCGTGGTGCCACCGGGGGGTGAAGAGCCCCGTATGCACTGTGCAGTGGATGGCGAGTGGCTGGTGCCCATTGGGCAGTGCCTGTGCCAGGCAGGCTACGAGAAGGTGGAGGATGCCTGCCAGGCCTGCTCGCCTGGATTTTTTAAGTTTGAGGCATCTGAGAGCCCCTGCTTGGAGTGCCCTGAGCACACGCTGCCATCCCCTGAGGGTGCCACCTCCTGCGAGTGTGAGGAAGGCTTCTTCCGGGCACCTCAGGACCCAGCGTCGATGCCTTGCACACGACCCCCTTCCGCCCCACACTACCTCACAGCCGTGGGCATGGGTGCCAAGGTGGAGCTGCGCTGGACGCCCCCTCAGGACAGCGGGGGCCGCGAGGACATTGTCTACAGCGTCACCTGCGAACAGTGCTGGCCCGAGTCTGGGGAATGCGGGCCGTGTGAGGCCAGTGTGCGCTACTCGGAGCCTCCTCACGGACTGACCCGCACCAGTGTGACAGTGAGCGACCTGGAGCCCCACATGAACTACACCTTCACCGTGGAGGCCCGCAATGGCGTCTCAGGCCTGGTAACCAGCCGCAGCTTCCGTACTGCCAGTGTCAGCATCAACCAGACAGAGCCCCCCAAGGTGAGGCTGGAGGGCCGCAGCACCACCTCGCTTAGCGTCTCCTGGAGCATCCCCCCGCCGCAGCAGAGCCGAGTGTGGAAGTACGAGGTCACTTACCGCAAGAAGGGAGACTCCAACAGCTACAATGTGCGCCGCACCGAGGGTTTCTCCGTGACCCTGGACGACCTGGCCCCAGACACCACCTACCTGGTCCAGGTGCAGGCACTGACGCAGGAGGGCCAGGGGGCCGGCAGCAAGGTGCACGAATTCCAGACGCTGTCCCCGGAGGGATCTGGCAACTTGGCGGTGATTGGCGGCGTGGCTGTCGGTGTGGTCCTGCTTCTGGTGCTGGCAGGAGTTGGCTTCTTTATCCACCGCAGGAGGAAGAACCAGCGTGCCCGCCAGTCCCCGGAGGACGTTTACTTCTCCAAGTCAGAACAACTGAAGCCCCTGAAGACATACGTGGACCCCCACACATATGAGGACCCCAACCAGGCTGTGTTGAAGTTCACTACCGAGATCCATCCATCCTGTGTCACTCGGCAGAAGGTGATCGGAGCAGGAGAGTTTGGGGAGGTGTACAAGGGCATGCTGAAGACATCCTCGGGGAAGAAGGAGGTGCCGGTGGCCATCAAGACGCTGAAAGCCGGCTACACAGAGAAGCAGCGAGTGGACTTCCTCGGCGAGGCCGGCATCATGGGCCAGTTCAGCCACCACAACATCATCCGCCTAGAGGGCGTCATCTCCAAATACAAGCCCATGATGATCATCACTGAGTACATGGAGAATGGGGCCCTGGACAAGTTCCTTCGGGAGAAGGATGGCGAGTTCAGCGTGCTGCAGCTGGTGGGCATGCTGCGGGGCATCGCAGCTGGCATGAAGTACCTGGCCAACATGAACTATGTGCACCGTGACCTGGCTGCCCGCAACATCCTCGTCAACAGCAACCTGGTCTGCAAGGTGTCTGACTTTGGCCTGTCCCGCGTGCTGGAGGACGACCCCGAGGCCACCTACACCACCAGTGGCGGCAAGATCCCCATCCGCTGGACCGCCCCGGAGGCCATTTCCTACCGGAAGTTCACCTCTGCCAGCGACGTGTGGAGCTTTGGCATTGTCATGTGGGAGGTGATGACCTATGGCGAGCGGCCCTACTGGGAGTTGTCCAACCACGAGGTGATGAAAGCCATCAATGATGGCTTCCGGCTCCCCACACCCATGGACTGCCCCTCCGCCATCTACCAGCTCATGATGCAGTGCTGGCAGCAGGAGCGTGCCCGCCGCCCCAAGTTCGCTGACATCGTCAGCATCCTGGACAAGCTCATTCGTGCCCCTGACTCCCTCAAGACCCTGGCTGACTTTGACCCCCGCGTGTCTATCCGGCTCCCCAGCACGAGCGGCTCGGAGGGGGTGCCCTTCCGCACGGTGTCCGAGTGGCTGGAGTCCATCAAGATGCAGCAGTATACGGAGCACTTCATGGCGGCCGGCTACACTGCCATCGAGAAGGTGGTGCAGATGACCAACGACGACATCAAGAGGATTGGGGTGCGGCTGCCCGGCCACCAGAAGCGCATCGCCTACAGCCTGCTGGGACTCAAGGACCAGGTGAACACTGTGGGGATCCCCATCTGAGCCTCGACAGGGCCTGGAGCCCCATCGGCCAAGAATACTTGAAGAAACAGAGTGGCCTCCCTGCTGTGCCATGCTGGGCCACTGGGGACTTTATTTATTTCTAGTTCTTTCCTCCCCCTGCAACTTCCGCTGAGGGGTCTCGGATGACACCCTGGCCTGAACTGAGGAGATGACCAGGGATGCTGGGCTGGGCCCTCTTTCCCTGCGAGACGCACACAGCTGAGCACTTAGCAGGCACCGCCACGTCCCAGCATCCCTGGAGCAGGAGCCCCGCCACAGCCTTCGGACAGACATATAGGATATTCCCAAGCCGACCTTCCCTCCGCCTTCTCCCACATGAGGCCATCTCAGGAGATGGAGGGCTTGGCCCAGCGCCAAGTAAACAGGGTACCTCAAGCCCCATTTCCTCACACTAAGAGGGCAGACTGTGAACTTGACTGGGTGAGACCCAAAGCGGTCCCTGTCCCTCTAGTGCCTTCTTTAGACCCTCGGGCCCCATCCTCATCCCTGACTGGCCAAACCCTTGCTTTCCTGGGCCTTTGCAAGATGCTTGGTTGTGTTGAGGTTTTTAAATATATATTTTGTACTTTGTGGAGAGAATGTGTGTGTGTGGCAGGGGGCCCCGCCAGGGCTGGGGACAGAGGGTGTCAAACATTCGTGAGCTGGGGACTCAGGGACCGGTGCTGCAGGAGTGTCCTGCCCATGCCCCAGTCGGCCCCATCTCTCATCCTTTTGGATAAGTTTCTATTCTGTCAGTGTTAAAGATTTTGTTTTGTTGGACATTTTTTTCGAATCTTAATTTATTATTTTTTTTATATTTATTGTTAGAAAATGACTTATTTCTGCTCTGGAATAAAGTTGCAGATGATTCAAACCG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 44980, GC content failed 1905, low tm 9258, high tm 25901, high any compl 10, high end compl 44, high repeat similarity 35, long poly-x seq 120, ok 7707
PRIMER_RIGHT_EXPLAIN=considered 45029, GC content failed 1837, low tm 9610, high tm 25601, high any compl 4, high end compl 9, high repeat similarity 9, long poly-x seq 114, ok 7845
PRIMER_PAIR_EXPLAIN=considered 1120, unacceptable product size 1057, high end compl 9, ok 54
PRIMER_PAIR_PENALTY=0.2810
PRIMER_LEFT_PENALTY=0.010990
PRIMER_RIGHT_PENALTY=0.270039
PRIMER_LEFT_SEQUENCE=CTCACACTAAGAGGGCAGACTGT
PRIMER_RIGHT_SEQUENCE=ATCTGCAACTTTATTCCAGAGCA
PRIMER_LEFT=3450,23
PRIMER_RIGHT=3909,23
PRIMER_LEFT_TM=59.989
PRIMER_RIGHT_TM=60.270
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=460
PRIMER_PAIR_PENALTY_1=0.2810
PRIMER_LEFT_1_PENALTY=0.010990
PRIMER_RIGHT_1_PENALTY=0.270039
PRIMER_LEFT_1_SEQUENCE=CTCACACTAAGAGGGCAGACTGT
PRIMER_RIGHT_1_SEQUENCE=CATCTGCAACTTTATTCCAGAGC
PRIMER_LEFT_1=3450,23
PRIMER_RIGHT_1=3910,23
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=461
PRIMER_PAIR_PENALTY_2=0.3234
PRIMER_LEFT_2_PENALTY=0.312402
PRIMER_RIGHT_2_PENALTY=0.010990
PRIMER_LEFT_2_SEQUENCE=ACAGCCTTCGGACAGACATATAG
PRIMER_RIGHT_2_SEQUENCE=ACAGTCTGCCCTCTTAGTGTGAG
PRIMER_LEFT_2=3319,23
PRIMER_RIGHT_2=3472,23
PRIMER_LEFT_2_TM=59.688
PRIMER_RIGHT_2_TM=59.989
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=4.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.3554
PRIMER_LEFT_3_PENALTY=0.344363
PRIMER_RIGHT_3_PENALTY=0.010990
PRIMER_LEFT_3_SEQUENCE=GCCACTGGGGACTTTATTTATTT
PRIMER_RIGHT_3_SEQUENCE=ACAGTCTGCCCTCTTAGTGTGAG
PRIMER_LEFT_3=3121,23
PRIMER_RIGHT_3=3472,23
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=352
PRIMER_PAIR_PENALTY_4=0.4049
PRIMER_LEFT_4_PENALTY=0.393933
PRIMER_RIGHT_4_PENALTY=0.010990
PRIMER_LEFT_4_SEQUENCE=ATATAGGATATTCCCAAGCCGAC
PRIMER_RIGHT_4_SEQUENCE=ACAGTCTGCCCTCTTAGTGTGAG
PRIMER_LEFT_4=3336,23
PRIMER_RIGHT_4=3472,23
PRIMER_LEFT_4_TM=59.606
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
PRIMER_PAIR_PENALTY_5=0.4200
PRIMER_LEFT_5_PENALTY=0.409035
PRIMER_RIGHT_5_PENALTY=0.010990
PRIMER_LEFT_5_SEQUENCE=ACATATAGGATATTCCCAAGCCG
PRIMER_RIGHT_5_SEQUENCE=ACAGTCTGCCCTCTTAGTGTGAG
PRIMER_LEFT_5=3334,23
PRIMER_RIGHT_5=3472,23
PRIMER_LEFT_5_TM=60.409
PRIMER_RIGHT_5_TM=59.989
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=11.4000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=139
PRIMER_PAIR_PENALTY_6=0.4305
PRIMER_LEFT_6_PENALTY=0.010990
PRIMER_RIGHT_6_PENALTY=0.419474
PRIMER_LEFT_6_SEQUENCE=CTCACACTAAGAGGGCAGACTGT
PRIMER_RIGHT_6_SEQUENCE=TAAAAACCTCAACACAACCAAGC
PRIMER_LEFT_6=3450,23
PRIMER_RIGHT_6=3614,23
PRIMER_LEFT_6_TM=59.989
PRIMER_RIGHT_6_TM=60.419
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=165
PRIMER_PAIR_PENALTY_7=0.4659
PRIMER_LEFT_7_PENALTY=0.195841
PRIMER_RIGHT_7_PENALTY=0.270039
PRIMER_LEFT_7_SEQUENCE=CCTCTAGTGCCTTCTTTAGACCC
PRIMER_RIGHT_7_SEQUENCE=ATCTGCAACTTTATTCCAGAGCA
PRIMER_LEFT_7=3509,23
PRIMER_RIGHT_7=3909,23
PRIMER_LEFT_7_TM=59.804
PRIMER_RIGHT_7_TM=60.270
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.1000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=401
PRIMER_PAIR_PENALTY_8=0.4659
PRIMER_LEFT_8_PENALTY=0.195841
PRIMER_RIGHT_8_PENALTY=0.270039
PRIMER_LEFT_8_SEQUENCE=CCCTCTAGTGCCTTCTTTAGACC
PRIMER_RIGHT_8_SEQUENCE=ATCTGCAACTTTATTCCAGAGCA
PRIMER_LEFT_8=3508,23
PRIMER_RIGHT_8=3909,23
PRIMER_LEFT_8_TM=59.804
PRIMER_RIGHT_8_TM=60.270
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=402
PRIMER_PAIR_PENALTY_9=0.4659
PRIMER_LEFT_9_PENALTY=0.195841
PRIMER_RIGHT_9_PENALTY=0.270039
PRIMER_LEFT_9_SEQUENCE=CCTCTAGTGCCTTCTTTAGACCC
PRIMER_RIGHT_9_SEQUENCE=CATCTGCAACTTTATTCCAGAGC
PRIMER_LEFT_9=3509,23
PRIMER_RIGHT_9=3910,23
PRIMER_LEFT_9_TM=59.804
PRIMER_RIGHT_9_TM=60.270
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.1000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=402
PRIMER_PAIR_PENALTY_10=0.4659
PRIMER_LEFT_10_PENALTY=0.195841
PRIMER_RIGHT_10_PENALTY=0.270039
PRIMER_LEFT_10_SEQUENCE=CCCTCTAGTGCCTTCTTTAGACC
PRIMER_RIGHT_10_SEQUENCE=CATCTGCAACTTTATTCCAGAGC
PRIMER_LEFT_10=3508,23
PRIMER_RIGHT_10=3910,23
PRIMER_LEFT_10_TM=59.804
PRIMER_RIGHT_10_TM=60.270
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=403
PRIMER_PAIR_PENALTY_11=0.5082
PRIMER_LEFT_11_PENALTY=0.312402
PRIMER_RIGHT_11_PENALTY=0.195841
PRIMER_LEFT_11_SEQUENCE=ACAGCCTTCGGACAGACATATAG
PRIMER_RIGHT_11_SEQUENCE=GGTCTAAAGAAGGCACTAGAGGG
PRIMER_LEFT_11=3319,23
PRIMER_RIGHT_11=3530,23
PRIMER_LEFT_11_TM=59.688
PRIMER_RIGHT_11_TM=59.804
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, LTR6 LTR from  human SSAV-related endogenous retroviral LTR-like element
PRIMER_LEFT_11_END_STABILITY=4.9000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=212
PRIMER_PAIR_PENALTY_12=0.5082
PRIMER_LEFT_12_PENALTY=0.312402
PRIMER_RIGHT_12_PENALTY=0.195841
PRIMER_LEFT_12_SEQUENCE=ACAGCCTTCGGACAGACATATAG
PRIMER_RIGHT_12_SEQUENCE=GGGTCTAAAGAAGGCACTAGAGG
PRIMER_LEFT_12=3319,23
PRIMER_RIGHT_12=3531,23
PRIMER_LEFT_12_TM=59.688
PRIMER_RIGHT_12_TM=59.804
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=4.9000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=213
PRIMER_PAIR_PENALTY_13=0.5402
PRIMER_LEFT_13_PENALTY=0.344363
PRIMER_RIGHT_13_PENALTY=0.195841
PRIMER_LEFT_13_SEQUENCE=GCCACTGGGGACTTTATTTATTT
PRIMER_RIGHT_13_SEQUENCE=GGTCTAAAGAAGGCACTAGAGGG
PRIMER_LEFT_13=3121,23
PRIMER_RIGHT_13=3530,23
PRIMER_LEFT_13_TM=59.656
PRIMER_RIGHT_13_TM=59.804
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.2000
PRIMER_RIGHT_13_END_STABILITY=9.4000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=410
PRIMER_PAIR_PENALTY_14=0.5402
PRIMER_LEFT_14_PENALTY=0.344363
PRIMER_RIGHT_14_PENALTY=0.195841
PRIMER_LEFT_14_SEQUENCE=GCCACTGGGGACTTTATTTATTT
PRIMER_RIGHT_14_SEQUENCE=GGGTCTAAAGAAGGCACTAGAGG
PRIMER_LEFT_14=3121,23
PRIMER_RIGHT_14=3531,23
PRIMER_LEFT_14_TM=59.656
PRIMER_RIGHT_14_TM=59.804
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.2000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=411
PRIMER_PAIR_PENALTY_15=0.5442
PRIMER_LEFT_15_PENALTY=0.278288
PRIMER_RIGHT_15_PENALTY=0.265933
PRIMER_LEFT_15_SEQUENCE=GAGTGTGGAAGTACGAGGTCACT
PRIMER_RIGHT_15_SEQUENCE=GGTAGTGAACTTCAACACAGCCT
PRIMER_LEFT_15=1506,23
PRIMER_RIGHT_15=1930,23
PRIMER_LEFT_15_TM=59.722
PRIMER_RIGHT_15_TM=59.734
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, reverse MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MER7B Nonautonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=8.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=425
PRIMER_PAIR_PENALTY_16=0.5720
PRIMER_LEFT_16_PENALTY=0.306034
PRIMER_RIGHT_16_PENALTY=0.265933
PRIMER_LEFT_16_SEQUENCE=AGAAGGGAGACTCCAACAGCTAC
PRIMER_RIGHT_16_SEQUENCE=GGTAGTGAACTTCAACACAGCCT
PRIMER_LEFT_16=1536,23
PRIMER_RIGHT_16=1930,23
PRIMER_LEFT_16_TM=60.306
PRIMER_RIGHT_16_TM=59.734
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=395
PRIMER_PAIR_PENALTY_17=0.5824
PRIMER_LEFT_17_PENALTY=0.312402
PRIMER_RIGHT_17_PENALTY=0.270039
PRIMER_LEFT_17_SEQUENCE=ACAGCCTTCGGACAGACATATAG
PRIMER_RIGHT_17_SEQUENCE=ATCTGCAACTTTATTCCAGAGCA
PRIMER_LEFT_17=3319,23
PRIMER_RIGHT_17=3909,23
PRIMER_LEFT_17_TM=59.688
PRIMER_RIGHT_17_TM=60.270
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_LEFT_17_END_STABILITY=4.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=591
PRIMER_PAIR_PENALTY_18=0.5824
PRIMER_LEFT_18_PENALTY=0.312402
PRIMER_RIGHT_18_PENALTY=0.270039
PRIMER_LEFT_18_SEQUENCE=ACAGCCTTCGGACAGACATATAG
PRIMER_RIGHT_18_SEQUENCE=CATCTGCAACTTTATTCCAGAGC
PRIMER_LEFT_18=3319,23
PRIMER_RIGHT_18=3910,23
PRIMER_LEFT_18_TM=59.688
PRIMER_RIGHT_18_TM=60.270
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse CER Human D22Z3 repetitive DNA (centromeric DNA)
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=4.9000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=592
PRIMER_PAIR_PENALTY_19=0.5898
PRIMER_LEFT_19_PENALTY=0.393933
PRIMER_RIGHT_19_PENALTY=0.195841
PRIMER_LEFT_19_SEQUENCE=ATATAGGATATTCCCAAGCCGAC
PRIMER_RIGHT_19_SEQUENCE=GGTCTAAAGAAGGCACTAGAGGG
PRIMER_LEFT_19=3336,23
PRIMER_RIGHT_19=3530,23
PRIMER_LEFT_19_TM=59.606
PRIMER_RIGHT_19_TM=59.804
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.6000
PRIMER_RIGHT_19_END_STABILITY=9.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=195
=