PRIMER_SEQUENCE_ID=NM_004438
SEQUENCE=AAGCGGCAGGAGCAGCGTTGGCACCGGCGAACCATGGCTGGGATTTTCTATTTCGCCCTATTTTCGTGTCTCTTCGGGATTTGCGACGCTGTCACAGGTTCCAGGGTATACCCCGCGAATGAAGTTACCTTATTGGATTCCAGATCTGTTCAGGGAGAACTTGGGTGGATAGCAAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGTATCATGGATGAAAAAAATACACCAATCCGAACCTACCAAGTGTGCAATGTGATGGAACCCAGCCAGAATAACTGGCTACGAACTGATTGGATCACCCGAGAAGGGGCTCAGAGGGTGTATATTGAGATTAAATTCACCTTGAGGGACTGCAATAGTCTTCCGGGCGTCATGGGGACTTGCAAGGAGACGTTTAACCTGTACTACTATGAATCAGACAACGACAAAGAGCGTTTCATCAGAGAGAACCAGTTTGTCAAAATTGACACCATTGCTGCTGATGAGAGCTTCACCCAAGTGGACATTGGTGACAGAATCATGAAGCTGAACACCGAGATCCGGGATGTAGGGCCATTAAGCAAAAAGGGGTTTTACCTGGCTTTTCAGGATGTGGGGGCCTGCATCGCCCTGGTATCAGTCCGTGTGTTCTATAAAAAGTGTCCACTCACAGTCCGCAATCTGGCCCAGTTTCCTGACACCATCACAGGGGCTGATACGTCTTCCCTGGTGGAAGTTCGAGGCTCCTGTGTCAACAACTCAGAAGAGAAAGATGTGCCAAAAATGTACTGTGGGGCAGATGGTGAATGGCTGGTACCCATTGGCAACTGCCTATGCAACGCTGGGCATGAGGAGCGGAGCGGAGAATGCCAAGCTTGCAAAATTGGATATTACAAGGCTCTCTCCACGGATGCCACCTGTGCCAAGTGCCCACCCCACAGCTACTCTGTCTGGGAAGGAGCCACCTCGTGCACCTGTGACCGAGGCTTTTTCAGAGCTGACAACGATGCTGCCTCTATGCCCTGCACCCGTCCACCATCTGCTCCCCTGAACTTGATTTCAAATGTCAACGAGACATCTGTGAACTTGGAATGGAGTAGCCCTCAGAATACAGGTGGCCGCCAGGACATTTCCTATAATGTGGTATGCAAGAAATGTGGAGCTGGTGACCCCAGCAAGTGCCGACCCTGTGGAAGTGGGGTCCACTACACCCCACAGCAGAATGGCTTGAAGACCACCAAAGTCTCCATCACTGACCTCCTAGCTCATACCAATTACACCTTTGAAATCTGGGCTGTGAATGGAGTGTCCAAATATAACCCTAACCCAGACCAATCAGTTTCTGTCACTGTGACCACCAACCAAGCAGCACCATCATCCATTGCTTTGGTCCAGGCTAAAGAAGTCACAAGATACAGTGTGGCACTGGCTTGGCTGGAACCAGATCGGCCCAATGGGGTAATCCTGGAATATGAAGTCAAGTATTATGAGAAGGATCAGAATGAGCGAAGCTATCGTATAGTTCGGACAGCTGCCAGGAACACAGATATCAAAGGCCTGAACCCTCTCACTTCCTATGTTTTCCACGTGCGAGCCAGGACAGCAGCTGGCTATGGAGACTTCAGTGAGCCCTTGGAGGTTACAACCAACACAGTGCCTTCCCGGATCATTGGAGATGGGGCTAACTCCACAGTCCTTCTGGTCTCTGTCTCGGGCAGTGTGGTGCTGGTGGTAATTCTCATTGCAGCTTTTGTCATCAGCCGGAGACGGAGTAAATACAGTAAAGCCAAACAAGAAGCGGATGAAGAGAAACATTTGAATCAAGGTGTAAGAACATATGTGGACCCCTTTACGTACGAAGATCCCAACCAAGCAGTGCGAGAGTTTGCCAAAGAAATTGACGCATCCTGCATTAAGATTGAAAAAGTTATAGGAGTTGGTGAATTTGGTGAGGTATGCAGTGGGCGTCTCAAAGTGCCTGGCAAGAGAGAGATCTGTGTGGCTATCAAGACTCTGAAAGCTGGTTATACAGACAAACAGAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTTTGACCATCCGAACATCATTCACTTGGAAGGCGTGGTCACTAAATGTAAACCAGTAATGATCATAACAGAGTACATGGAGAATGGCTCCTTGGATGCATTCCTCAGGAAAAATGATGGCAGATTTACAGTCATTCAGCTGGTGGGCATGCTTCGTGGCATTGGGTCTGGGATGAAGTATTTATCTGATATGAGCTATGTGCATCGTGATCTGGCCGCACGGAACATCCTGGTGAACAGCAACTTGGTCTGCAAAGTGTCTGATTTTGGCATGTCCCGAGTGCTTGAGGATGATCCGGAAGCAGCTTACACCACCAGGGGTGGCAAGATTCCTATCCGGTGGACTGCGCCAGAAGCAATTGCCTATCGTAAATTCACATCAGCAAGTGATGTATGGAGCTATGGAATCGTTATGTGGGAAGTGATGTCGTACGGGGAGAGGCCCTATTGGGATATGTCCAATCAAGATGTGATTAAAGCCATTGAGGAAGGCTATCGGTTACCCCCTCCAATGGACTGCCCCATTGCGCTCCACCAGCTGATGCTAGACTGCTGGCAGAAGGAGAGGAGCGACAGGCCTAAATTTGGGCAGATTGTCAACATGTTGGACAAACTCATCCGCAACCCCAACAGCTTGAAGAGGACAGGGACGGAGAGCTCCAGACCTAACACTGCCTTGTTGGATCCAAGCTCCCCTGAATTCTCTGCTGTGGTATCAGTGGGCGATTGGCTCCAGGCCATTAAAATGGACCGGTATAAGGATAACTTCACAGCTGCTGGTTATACCACACTAGAGGCTGTGGTGCACGTGAACCAGGAGGACCTGGCAAGAATTGGTATCACAGCCATCACGCACCAGAATAAGATTTTGAGCAGTGTCCAGGCAATGCGAACCCAAATGCAGCAGATGCACGGCAGAATGGTTCCCGTCTGAGCCAGTACTGAATAAACTCAAAACTCTTGAAATTAGTTTACCTCATCCATGCACTTTAATTGAAGAACTGCACTTTTTTTACTTCGTCTTCGCCCTCTGAAATTAAAGAAATG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 35920, GC content failed 60, low tm 14237, high tm 13382, high any compl 8, high end compl 40, high repeat similarity 57, long poly-x seq 22, ok 8114
PRIMER_RIGHT_EXPLAIN=considered 35744, GC content failed 68, low tm 14522, high tm 12927, high any compl 2, high end compl 14, high repeat similarity 9, long poly-x seq 44, ok 8158
PRIMER_PAIR_EXPLAIN=considered 672, unacceptable product size 606, high end compl 5, ok 61
PRIMER_PAIR_PENALTY=0.0497
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.035468
PRIMER_LEFT_SEQUENCE=AACACAGATATCAAAGGCCTGAA
PRIMER_RIGHT_SEQUENCE=AAATACTTCATCCCAGACCCAAT
PRIMER_LEFT=1521,23
PRIMER_RIGHT=2242,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.965
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=722
PRIMER_PAIR_PENALTY_1=0.0739
PRIMER_LEFT_1_PENALTY=0.038458
PRIMER_RIGHT_1_PENALTY=0.035468
PRIMER_LEFT_1_SEQUENCE=CAAACAGAGGAGAGACTTCCTGA
PRIMER_RIGHT_1_SEQUENCE=AAATACTTCATCCCAGACCCAAT
PRIMER_LEFT_1=2015,23
PRIMER_RIGHT_1=2242,23
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.0900
PRIMER_LEFT_2_PENALTY=0.054491
PRIMER_RIGHT_2_PENALTY=0.035468
PRIMER_LEFT_2_SEQUENCE=AAGGCGTGGTCACTAAATGTAAA
PRIMER_RIGHT_2_SEQUENCE=AAATACTTCATCCCAGACCCAAT
PRIMER_LEFT_2=2089,23
PRIMER_RIGHT_2=2242,23
PRIMER_LEFT_2_TM=59.946
PRIMER_RIGHT_2_TM=59.965
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.0957
PRIMER_LEFT_3_PENALTY=0.014220
PRIMER_RIGHT_3_PENALTY=0.081493
PRIMER_LEFT_3_SEQUENCE=AACACAGATATCAAAGGCCTGAA
PRIMER_RIGHT_3_SEQUENCE=TAGCTCCATACATCACTTGCTGA
PRIMER_LEFT_3=1521,23
PRIMER_RIGHT_3=2461,23
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=941
PRIMER_PAIR_PENALTY_4=0.1074
PRIMER_LEFT_4_PENALTY=0.071885
PRIMER_RIGHT_4_PENALTY=0.035468
PRIMER_LEFT_4_SEQUENCE=TGTGTGGCTATCAAGACTCTGAA
PRIMER_RIGHT_4_SEQUENCE=AAATACTTCATCCCAGACCCAAT
PRIMER_LEFT_4=1977,23
PRIMER_RIGHT_4=2242,23
PRIMER_LEFT_4_TM=59.928
PRIMER_RIGHT_4_TM=59.965
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=266
PRIMER_PAIR_PENALTY_5=0.1164
PRIMER_LEFT_5_PENALTY=0.102207
PRIMER_RIGHT_5_PENALTY=0.014220
PRIMER_LEFT_5_SEQUENCE=TGGATATTACAAGGCTCTCTCCA
PRIMER_RIGHT_5_SEQUENCE=TTCAGGCCTTTGATATCTGTGTT
PRIMER_LEFT_5=866,23
PRIMER_RIGHT_5=1543,23
PRIMER_LEFT_5_TM=60.102
PRIMER_RIGHT_5_TM=60.014
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse XTR Repetitive element X-2 from human alpha-fetoprotein gene
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=8.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=678
PRIMER_PAIR_PENALTY_6=0.1200
PRIMER_LEFT_6_PENALTY=0.038458
PRIMER_RIGHT_6_PENALTY=0.081493
PRIMER_LEFT_6_SEQUENCE=CAAACAGAGGAGAGACTTCCTGA
PRIMER_RIGHT_6_SEQUENCE=TAGCTCCATACATCACTTGCTGA
PRIMER_LEFT_6=2015,23
PRIMER_RIGHT_6=2461,23
PRIMER_LEFT_6_TM=60.038
PRIMER_RIGHT_6_TM=59.919
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=447
PRIMER_PAIR_PENALTY_7=0.1264
PRIMER_LEFT_7_PENALTY=0.071885
PRIMER_RIGHT_7_PENALTY=0.054491
PRIMER_LEFT_7_SEQUENCE=TGTGTGGCTATCAAGACTCTGAA
PRIMER_RIGHT_7_SEQUENCE=TTTACATTTAGTGACCACGCCTT
PRIMER_LEFT_7=1977,23
PRIMER_RIGHT_7=2111,23
PRIMER_LEFT_7_TM=59.928
PRIMER_RIGHT_7_TM=59.946
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=9.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=135
PRIMER_PAIR_PENALTY_8=0.1360
PRIMER_LEFT_8_PENALTY=0.054491
PRIMER_RIGHT_8_PENALTY=0.081493
PRIMER_LEFT_8_SEQUENCE=AAGGCGTGGTCACTAAATGTAAA
PRIMER_RIGHT_8_SEQUENCE=TAGCTCCATACATCACTTGCTGA
PRIMER_LEFT_8=2089,23
PRIMER_RIGHT_8=2461,23
PRIMER_LEFT_8_TM=59.946
PRIMER_RIGHT_8_TM=59.919
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.0000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=373
PRIMER_PAIR_PENALTY_9=0.1527
PRIMER_LEFT_9_PENALTY=0.014220
PRIMER_RIGHT_9_PENALTY=0.138433
PRIMER_LEFT_9_SEQUENCE=AACACAGATATCAAAGGCCTGAA
PRIMER_RIGHT_9_SEQUENCE=GATCACGATGCACATAGCTCATA
PRIMER_LEFT_9=1521,23
PRIMER_RIGHT_9=2271,23
PRIMER_LEFT_9_TM=60.014
PRIMER_RIGHT_9_TM=60.138
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=7.0000
PRIMER_RIGHT_9_END_STABILITY=5.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=751
PRIMER_PAIR_PENALTY_10=0.1527
PRIMER_LEFT_10_PENALTY=0.014220
PRIMER_RIGHT_10_PENALTY=0.138433
PRIMER_LEFT_10_SEQUENCE=AACACAGATATCAAAGGCCTGAA
PRIMER_RIGHT_10_SEQUENCE=TCACGATGCACATAGCTCATATC
PRIMER_LEFT_10=1521,23
PRIMER_RIGHT_10=2269,23
PRIMER_LEFT_10_TM=60.014
PRIMER_RIGHT_10_TM=60.138
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=5.5000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=749
PRIMER_PAIR_PENALTY_11=0.1534
PRIMER_LEFT_11_PENALTY=0.071885
PRIMER_RIGHT_11_PENALTY=0.081493
PRIMER_LEFT_11_SEQUENCE=TGTGTGGCTATCAAGACTCTGAA
PRIMER_RIGHT_11_SEQUENCE=TAGCTCCATACATCACTTGCTGA
PRIMER_LEFT_11=1977,23
PRIMER_RIGHT_11=2461,23
PRIMER_LEFT_11_TM=59.928
PRIMER_RIGHT_11_TM=59.919
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=485
PRIMER_PAIR_PENALTY_12=0.1769
PRIMER_LEFT_12_PENALTY=0.038458
PRIMER_RIGHT_12_PENALTY=0.138433
PRIMER_LEFT_12_SEQUENCE=CAAACAGAGGAGAGACTTCCTGA
PRIMER_RIGHT_12_SEQUENCE=GATCACGATGCACATAGCTCATA
PRIMER_LEFT_12=2015,23
PRIMER_RIGHT_12=2271,23
PRIMER_LEFT_12_TM=60.038
PRIMER_RIGHT_12_TM=60.138
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=5.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=257
PRIMER_PAIR_PENALTY_13=0.1769
PRIMER_LEFT_13_PENALTY=0.038458
PRIMER_RIGHT_13_PENALTY=0.138433
PRIMER_LEFT_13_SEQUENCE=CAAACAGAGGAGAGACTTCCTGA
PRIMER_RIGHT_13_SEQUENCE=TCACGATGCACATAGCTCATATC
PRIMER_LEFT_13=2015,23
PRIMER_RIGHT_13=2269,23
PRIMER_LEFT_13_TM=60.038
PRIMER_RIGHT_13_TM=60.138
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=5.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=255
PRIMER_PAIR_PENALTY_14=0.1818
PRIMER_LEFT_14_PENALTY=0.038458
PRIMER_RIGHT_14_PENALTY=0.143370
PRIMER_LEFT_14_SEQUENCE=CAAACAGAGGAGAGACTTCCTGA
PRIMER_RIGHT_14_SEQUENCE=GGCTTTAATCACATCTTGATTGG
PRIMER_LEFT_14=2015,23
PRIMER_RIGHT_14=2540,23
PRIMER_LEFT_14_TM=60.038
PRIMER_RIGHT_14_TM=59.857
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=526
PRIMER_PAIR_PENALTY_15=0.1847
PRIMER_LEFT_15_PENALTY=0.102207
PRIMER_RIGHT_15_PENALTY=0.082508
PRIMER_LEFT_15_SEQUENCE=TGGATATTACAAGGCTCTCTCCA
PRIMER_RIGHT_15_SEQUENCE=ATACGATAGCTTCGCTCATTCTG
PRIMER_LEFT_15=866,23
PRIMER_RIGHT_15=1501,23
PRIMER_LEFT_15_TM=60.102
PRIMER_RIGHT_15_TM=59.917
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MER1A Nonautonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=636
PRIMER_PAIR_PENALTY_16=0.1850
PRIMER_LEFT_16_PENALTY=0.149559
PRIMER_RIGHT_16_PENALTY=0.035468
PRIMER_LEFT_16_SEQUENCE=ATTCTCATTGCAGCTTTTGTCAT
PRIMER_RIGHT_16_SEQUENCE=AAATACTTCATCCCAGACCCAAT
PRIMER_LEFT_16=1716,23
PRIMER_RIGHT_16=2242,23
PRIMER_LEFT_16_TM=60.150
PRIMER_RIGHT_16_TM=59.965
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.3000
PRIMER_RIGHT_16_END_STABILITY=8.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=527
PRIMER_PAIR_PENALTY_17=0.1929
PRIMER_LEFT_17_PENALTY=0.054491
PRIMER_RIGHT_17_PENALTY=0.138433
PRIMER_LEFT_17_SEQUENCE=AAGGCGTGGTCACTAAATGTAAA
PRIMER_RIGHT_17_SEQUENCE=TCACGATGCACATAGCTCATATC
PRIMER_LEFT_17=2089,23
PRIMER_RIGHT_17=2269,23
PRIMER_LEFT_17_TM=59.946
PRIMER_RIGHT_17_TM=60.138
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.0000
PRIMER_RIGHT_17_END_STABILITY=5.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=181
PRIMER_PAIR_PENALTY_18=0.1929
PRIMER_LEFT_18_PENALTY=0.054491
PRIMER_RIGHT_18_PENALTY=0.138433
PRIMER_LEFT_18_SEQUENCE=AAGGCGTGGTCACTAAATGTAAA
PRIMER_RIGHT_18_SEQUENCE=GATCACGATGCACATAGCTCATA
PRIMER_LEFT_18=2089,23
PRIMER_RIGHT_18=2271,23
PRIMER_LEFT_18_TM=59.946
PRIMER_RIGHT_18_TM=60.138
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse LTR2 LTR from human endogenous retrovirus (4-14), 3' long terminal repeat
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=6.0000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=183
PRIMER_PAIR_PENALTY_19=0.1979
PRIMER_LEFT_19_PENALTY=0.054491
PRIMER_RIGHT_19_PENALTY=0.143370
PRIMER_LEFT_19_SEQUENCE=AAGGCGTGGTCACTAAATGTAAA
PRIMER_RIGHT_19_SEQUENCE=GGCTTTAATCACATCTTGATTGG
PRIMER_LEFT_19=2089,23
PRIMER_RIGHT_19=2540,23
PRIMER_LEFT_19_TM=59.946
PRIMER_RIGHT_19_TM=59.857
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=6.0000
PRIMER_RIGHT_19_END_STABILITY=8.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=452
=