PRIMER_SEQUENCE_ID=NM_004441
SEQUENCE=CACATGCACACCCACACCCACGCGCGCCCGCACCGCCCCACGCGCACACACTCCTGCCCACGCCCACGCAGCGCTCCGGGAAGTCCGGTCCGGGCGAGAGCGCGAAAGGATACCGAGAAGCCACCCGCGGAGAGCGCAGCGGCGCCCTGGGACGCGGCGCTCTCCCGGCGCTGCTGCCTCGGCTTGGTCTCGGCCTGCGGGCCGTCGGCCGGCGATGGCCCTGGATTATCTACTACTGCTCCTCCTGGCATCCGCAGTGGCTGCGATGGAAGAAACGTTAATGGACACCAGAACGGCTACTGCAGAGCTGGGCTGGACGGCCAATCCTGCGTCCGGGTGGGAAGAAGTCAGTGGCTACGATGAAAACCTGAACACCATCCGCACCTACCAGGTGTGCAATGTCTTCGAGCCCAACCAGAACAATTGGCTGCTCACCACCTTCATCAACCGGCGGGGGGCCCATCGCATCTACACAGAGATGCGCTTCACTGTGAGAGACTGCAGCAGCCTCCCTAATGTCCCAGGATCCTGCAAGGAGACCTTCAACTTGTATTACTATGAGACTGACTCTGTCATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAGGCCCCCTACCTCAAAGTAGACACCATTGCTGCAGATGAGAGCTTCTCCCAGGTGGACTTTGGGGGAAGGCTGATGAAGGTAAACACAGAAGTCAGGAGCTTTGGGCCTCTTACTCGGAATGGTTTTTACCTCGCTTTTCAGGATTATGGAGCCTGTATGTCTCTTCTTTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATTGTGCAAAATTTTGCAGTGTTTCCAGAGACTATGACAGGGGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCCAACGCAGAGGAAGTGGACGTGCCCATCAAACTCTACTGCAACGGGGATGGGGAATGGATGGTGCCTATTGGGCGATGCACCTGCAAGCCTGGCTATGAGCCTGAGAACAGCGTGGCATGCAAGGCTTGCCCTGCAGGGACATTCAAGGCCAGCCAGGAAGCTGAAGGCTGCTCCCACTGCCCCTCCAACAGCCGCTCCCCTGCAGAGGCGTCTCCCATCTGCACCTGTCGGACCGGTTATTACCGAGCGGACTTTGACCCTCCAGAAGTGGCATGCACTAGCGTCCCATCAGGTCCCCGCAATGTTATCTCCATCGTCAATGAGACGTCCATCATTCTGGAGTGGCACCCTCCAAGGGAGACAGGTGGGCGGGATGATGTGACCTACAACATCATCTGCAAAAAGTGCCGGGCAGACCGCCGGAGCTGCTCCCGCTGTGACGACAATGTGGAGTTTGTGCCCAGGCAGCTGGGCCTGACGGAGTGCCGCGTCTCCATCAGCAGCCTGTGGGCCCACACCCCCTACACCTTTGACATCCAGGCCATCAATGGAGTCTCCAGCAAGAGTCCCTTCCCCCCACAGCACGTCTCTGTCAACATCACCACAAACCAAGCCGCCCCCTCCACCGTTCCCATCATGCACCAAGTCAGTGCCACTATGAGGAGCATCACCTTGTCATGGCCACAGCCGGAGCAGCCCAATGGCATCATCCTGGACTATGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCCTCCATGGCCAGGAGTCAGACCAACACAGCAAGGATTGATGGGCTGCGGCCTGGCATGGTATATGTGGTACAGGTGCGTGCCCGCACTGTTGCTGGCTACGGCAAGTTCAGTGGCAAGATGTGCTTCCAGACTCTGACTGACGATGATTACAAGTCAGAGCTGAGGGAGCAGCTGCCCCTGATTGCTGGCTCGGCAGCGGCCGGGGTCGTGTTCGTTGTGTCCTTGGTGGCCATCTCTATCGTCTGTAGCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAGCATTACAGCACAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCCTTCACTTATGAGGATCCCAACGAAGCTGTCCGGGAGTTTGCCAAGGAGATTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTTGGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAAGAGGGAAATCTACGTGGCCATCAAGACCCTGAAGGCAGGGTACTCGGAGAAGCAGCGTCGGGACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCATCCTAACATCATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCACAGAGTTCATGGAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACGGGCAGTTCACCGTGATCCAGCTTGTGGGTATGCTCAGGGGCATCGCTGCTGGCATGAAGTACCTGGCTGAGATGAATTATGTGCATCGGGACCTGGCTGCTAGGAACATTCTGGTCAACAGTAACCTGGTGTGCAAGGTGTCCGACTTTGGCCTCTCCCGCTACCTCCAGGATGACACCTCAGATCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAGAGGCCATCGCCTACCGCAAGTTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCATGTGGGAAGTCATGTCATTTGGAGAGAGACCCTATTGGGATATGTCCAACCAAGATGTCATCAATGCCATCGAGCAGGACTACCGGCTGCCCCCACCCATGGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGTTGGCAGAAGGACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAGATGATCCGGAACCCGGCAAGTCTCAAGACTGTGGCAACCATCACCGCCGTGCCTTCCCAGCCCCTGCTCGACCGCTCCATCCCAGACTTCACGGCCTTTACCACCGTGGATGACTGGCTCAGCGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCACTGCTGGCTTCACCTCCCTCCAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGCATCACCTTGGCAGGCCATCAGAAGAAGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCAACGGCAATGGCATGAGAACTCTTGTTTCTTGGGGAAGGAGAGGAGGGAAAAGGACCAGGGTCAAGGGGGACCAGAGGTTGACCACTGTGGAATGTACTGGAGAGACTGGCTTCTCAGCTGAGGAATGCATTTCCATCAGTGAAGAATCAACCGGACCTGTTGCTAGCAGGCAATCTCCATTTCTCAGTGACAGAAGCATGTTTGAGATGCCGTGGGAAACCAAATATATAATAATAAAAATATAAAAAGGTGATGTTCAACAGAAGTGAAGACAAAACAATATGCATCAGGAGAACAAGAGTAAACCCAGCTCCCACTCTCAGTGGGCTGCAGTTGCCCAACCACAGGAAGAAAGGGAAGGAGGTAGAGGGAAGAAACAGAAGCAGTGTTCCATTTTCTTCCTCACCAATGACATTCTTTTCTTTTCTCCTTTCGTACTCCTCCCTGAGAGTCCCCTCCCTTCTCCCACACTCGTTTCCCTTTGCTCATGACTCCTGTAGGGAAGTTTCTTCAAACAAAACCCAGCTCCTGAGTCTCCAGATGTTGTTCTGTCAGTTGCCAAAGGACTTTGCTGACCACTGCATGGGGATCCAACCAATTCAATTAATGTCTTCATATTGAAGAAGAGATGTACCTTCAATTGAAAACCTCGTTTTTCTTTTGTTTGCATTTTCTGCAAAAAGGAAAAAGAAACCACAAATTGGGGAATTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 44895, GC content failed 1428, low tm 13931, high tm 20180, high any compl 1, high end compl 35, high repeat similarity 35, long poly-x seq 42, ok 9243
PRIMER_RIGHT_EXPLAIN=considered 44898, GC content failed 1152, low tm 14494, high tm 19686, high end compl 7, high repeat similarity 11, long poly-x seq 42, ok 9506
PRIMER_PAIR_EXPLAIN=considered 849, unacceptable product size 795, high any compl 1, high end compl 4, ok 49
PRIMER_PAIR_PENALTY=0.0550
PRIMER_LEFT_PENALTY=0.045432
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=GTGGAATGTACTGGAGAGACTGG
PRIMER_RIGHT_SEQUENCE=AGAATGTCATTGGTGAGGAAGAA
PRIMER_LEFT=3240,23
PRIMER_RIGHT=3571,23
PRIMER_LEFT_TM=60.045
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=332
PRIMER_PAIR_PENALTY_1=0.0550
PRIMER_LEFT_1_PENALTY=0.045432
PRIMER_RIGHT_1_PENALTY=0.009608
PRIMER_LEFT_1_SEQUENCE=GTGGAATGTACTGGAGAGACTGG
PRIMER_RIGHT_1_SEQUENCE=AAGAATGTCATTGGTGAGGAAGA
PRIMER_LEFT_1=3240,23
PRIMER_RIGHT_1=3572,23
PRIMER_LEFT_1_TM=60.045
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=333
PRIMER_PAIR_PENALTY_2=0.0766
PRIMER_LEFT_2_PENALTY=0.067019
PRIMER_RIGHT_2_PENALTY=0.009608
PRIMER_LEFT_2_SEQUENCE=GTACTGGAGAGACTGGCTTCTCA
PRIMER_RIGHT_2_SEQUENCE=AGAATGTCATTGGTGAGGAAGAA
PRIMER_LEFT_2=3247,23
PRIMER_RIGHT_2=3571,23
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=325
PRIMER_PAIR_PENALTY_3=0.0996
PRIMER_LEFT_3_PENALTY=0.079770
PRIMER_RIGHT_3_PENALTY=0.019823
PRIMER_LEFT_3_SEQUENCE=AAGAAACGTTAATGGACACCAGA
PRIMER_RIGHT_3_SEQUENCE=ATCCTGAAAAGCGAGGTAAAAAC
PRIMER_LEFT_3=269,23
PRIMER_RIGHT_3=750,23
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.020
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=482
PRIMER_PAIR_PENALTY_4=0.0996
PRIMER_LEFT_4_PENALTY=0.079770
PRIMER_RIGHT_4_PENALTY=0.019823
PRIMER_LEFT_4_SEQUENCE=AGAAACGTTAATGGACACCAGAA
PRIMER_RIGHT_4_SEQUENCE=ATCCTGAAAAGCGAGGTAAAAAC
PRIMER_LEFT_4=270,23
PRIMER_RIGHT_4=750,23
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=481
PRIMER_PAIR_PENALTY_5=0.0996
PRIMER_LEFT_5_PENALTY=0.079770
PRIMER_RIGHT_5_PENALTY=0.019823
PRIMER_LEFT_5_SEQUENCE=GAAGAAACGTTAATGGACACCAG
PRIMER_RIGHT_5_SEQUENCE=ATCCTGAAAAGCGAGGTAAAAAC
PRIMER_LEFT_5=268,23
PRIMER_RIGHT_5=750,23
PRIMER_LEFT_5_TM=59.920
PRIMER_RIGHT_5_TM=60.020
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=483
PRIMER_PAIR_PENALTY_6=0.1030
PRIMER_LEFT_6_PENALTY=0.079770
PRIMER_RIGHT_6_PENALTY=0.023182
PRIMER_LEFT_6_SEQUENCE=AAGAAACGTTAATGGACACCAGA
PRIMER_RIGHT_6_SEQUENCE=TACAGGCTCCATAATCCTGAAAA
PRIMER_LEFT_6=269,23
PRIMER_RIGHT_6=763,23
PRIMER_LEFT_6_TM=59.920
PRIMER_RIGHT_6_TM=59.977
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=495
PRIMER_PAIR_PENALTY_7=0.1030
PRIMER_LEFT_7_PENALTY=0.079770
PRIMER_RIGHT_7_PENALTY=0.023182
PRIMER_LEFT_7_SEQUENCE=AGAAACGTTAATGGACACCAGAA
PRIMER_RIGHT_7_SEQUENCE=TACAGGCTCCATAATCCTGAAAA
PRIMER_LEFT_7=270,23
PRIMER_RIGHT_7=763,23
PRIMER_LEFT_7_TM=59.920
PRIMER_RIGHT_7_TM=59.977
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.0000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=494
PRIMER_PAIR_PENALTY_8=0.1030
PRIMER_LEFT_8_PENALTY=0.079770
PRIMER_RIGHT_8_PENALTY=0.023182
PRIMER_LEFT_8_SEQUENCE=GAAGAAACGTTAATGGACACCAG
PRIMER_RIGHT_8_SEQUENCE=TACAGGCTCCATAATCCTGAAAA
PRIMER_LEFT_8=268,23
PRIMER_RIGHT_8=763,23
PRIMER_LEFT_8_TM=59.920
PRIMER_RIGHT_8_TM=59.977
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.3000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=496
PRIMER_PAIR_PENALTY_9=0.1126
PRIMER_LEFT_9_PENALTY=0.100593
PRIMER_RIGHT_9_PENALTY=0.012045
PRIMER_LEFT_9_SEQUENCE=GATTACAAGTCAGAGCTGAGGGA
PRIMER_RIGHT_9_SEQUENCE=GCTGTACACAGCCTCTTTGCTAT
PRIMER_LEFT_9=1801,23
PRIMER_RIGHT_9=1941,23
PRIMER_LEFT_9_TM=59.899
PRIMER_RIGHT_9_TM=59.988
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=141
PRIMER_PAIR_PENALTY_10=0.1190
PRIMER_LEFT_10_PENALTY=0.100593
PRIMER_RIGHT_10_PENALTY=0.018372
PRIMER_LEFT_10_SEQUENCE=GATTACAAGTCAGAGCTGAGGGA
PRIMER_RIGHT_10_SEQUENCE=CTTATCGCTGTACACAGCCTCTT
PRIMER_LEFT_10=1801,23
PRIMER_RIGHT_10=1947,23
PRIMER_LEFT_10_TM=59.899
PRIMER_RIGHT_10_TM=59.982
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=147
PRIMER_PAIR_PENALTY_11=0.1211
PRIMER_LEFT_11_PENALTY=0.100593
PRIMER_RIGHT_11_PENALTY=0.020535
PRIMER_LEFT_11_SEQUENCE=GATTACAAGTCAGAGCTGAGGGA
PRIMER_RIGHT_11_SEQUENCE=AAGATACATCAATCTCCTTGGCA
PRIMER_LEFT_11=1801,23
PRIMER_RIGHT_11=2065,23
PRIMER_LEFT_11_TM=59.899
PRIMER_RIGHT_11_TM=59.979
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=10.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=265
PRIMER_PAIR_PENALTY_12=0.1577
PRIMER_LEFT_12_PENALTY=0.148090
PRIMER_RIGHT_12_PENALTY=0.009608
PRIMER_LEFT_12_SEQUENCE=AGAACTCTTGTTTCTTGGGGAAG
PRIMER_RIGHT_12_SEQUENCE=AGAATGTCATTGGTGAGGAAGAA
PRIMER_LEFT_12=3168,23
PRIMER_RIGHT_12=3571,23
PRIMER_LEFT_12_TM=60.148
PRIMER_RIGHT_12_TM=59.990
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=404
PRIMER_PAIR_PENALTY_13=0.1577
PRIMER_LEFT_13_PENALTY=0.148090
PRIMER_RIGHT_13_PENALTY=0.009608
PRIMER_LEFT_13_SEQUENCE=AGAACTCTTGTTTCTTGGGGAAG
PRIMER_RIGHT_13_SEQUENCE=AAGAATGTCATTGGTGAGGAAGA
PRIMER_LEFT_13=3168,23
PRIMER_RIGHT_13=3572,23
PRIMER_LEFT_13_TM=60.148
PRIMER_RIGHT_13_TM=59.990
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=405
PRIMER_PAIR_PENALTY_14=0.1649
PRIMER_LEFT_14_PENALTY=0.144318
PRIMER_RIGHT_14_PENALTY=0.020535
PRIMER_LEFT_14_SEQUENCE=ATGAAGTACCTGGCTGAGATGAA
PRIMER_RIGHT_14_SEQUENCE=CCCTCATAGAATGAATGCTGTTC
PRIMER_LEFT_14=2407,23
PRIMER_RIGHT_14=3133,23
PRIMER_LEFT_14_TM=60.144
PRIMER_RIGHT_14_TM=59.979
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=727
PRIMER_PAIR_PENALTY_15=0.1815
PRIMER_LEFT_15_PENALTY=0.045432
PRIMER_RIGHT_15_PENALTY=0.136051
PRIMER_LEFT_15_SEQUENCE=GTGGAATGTACTGGAGAGACTGG
PRIMER_RIGHT_15_SEQUENCE=AATGTCATTGGTGAGGAAGAAAA
PRIMER_LEFT_15=3240,23
PRIMER_RIGHT_15=3569,23
PRIMER_LEFT_15_TM=60.045
PRIMER_RIGHT_15_TM=59.864
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.3000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=330
PRIMER_PAIR_PENALTY_16=0.1815
PRIMER_LEFT_16_PENALTY=0.045432
PRIMER_RIGHT_16_PENALTY=0.136051
PRIMER_LEFT_16_SEQUENCE=GTGGAATGTACTGGAGAGACTGG
PRIMER_RIGHT_16_SEQUENCE=AAAAGAATGTCATTGGTGAGGAA
PRIMER_LEFT_16=3240,23
PRIMER_RIGHT_16=3574,23
PRIMER_LEFT_16_TM=60.045
PRIMER_RIGHT_16_TM=59.864
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=34.783
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=335
PRIMER_PAIR_PENALTY_17=0.1831
PRIMER_LEFT_17_PENALTY=0.173530
PRIMER_RIGHT_17_PENALTY=0.009608
PRIMER_LEFT_17_SEQUENCE=CTAGATAAGATGATCCGGAACCC
PRIMER_RIGHT_17_SEQUENCE=AGAATGTCATTGGTGAGGAAGAA
PRIMER_LEFT_17=2845,23
PRIMER_RIGHT_17=3571,23
PRIMER_LEFT_17_TM=60.174
PRIMER_RIGHT_17_TM=59.990
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.4000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=727
PRIMER_PAIR_PENALTY_18=0.1831
PRIMER_LEFT_18_PENALTY=0.173530
PRIMER_RIGHT_18_PENALTY=0.009608
PRIMER_LEFT_18_SEQUENCE=CTAGATAAGATGATCCGGAACCC
PRIMER_RIGHT_18_SEQUENCE=AAGAATGTCATTGGTGAGGAAGA
PRIMER_LEFT_18=2845,23
PRIMER_RIGHT_18=3572,23
PRIMER_LEFT_18_TM=60.174
PRIMER_RIGHT_18_TM=59.990
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=9.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=728
PRIMER_PAIR_PENALTY_19=0.1842
PRIMER_LEFT_19_PENALTY=0.174570
PRIMER_RIGHT_19_PENALTY=0.009608
PRIMER_LEFT_19_SEQUENCE=AGAACAAGAGTAAACCCAGCTCC
PRIMER_RIGHT_19_SEQUENCE=AGAATGTCATTGGTGAGGAAGAA
PRIMER_LEFT_19=3445,23
PRIMER_RIGHT_19=3571,23
PRIMER_LEFT_19_TM=60.175
PRIMER_RIGHT_19_TM=59.990
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MER4C a consensus
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=127
=