PRIMER_SEQUENCE_ID=NM_004444
SEQUENCE=CGTCCACCCGCCCAGGGAGAGTCAGACCTGGGGGGGCGAGGGCCCCCCAAACTCAGTTCGGATCCTACCCGAGTGAGGCGGCGCCATGGAGCTCCGGGTGCTGCTCTGCTGGGCTTCGTTGGCCGCAGCTTTGGAAGAGACCCTGCTGAACACAAAATTGGAAACTGCTGATCTGAAGTGGGTGACATTCCCTCAGGTGGACGGGCAGTGGGAGGAACTGAGCGGCCTGGATGAGGAACAGCACAGCGTGCGCACCTACGAAGTGTGTGAAGTGCAGCGTGCCCCGGGCCAGGCCCACTGGCTTCGCACAGGTTGGGTCCCACGGCGGGGCGCCGTCCACGTGTACGCCACGCTGCGCTTCACCATGCTCGAGTGCCTGTCCCTGCCTCGGGCTGGGCGCTCCTGCAAGGAGACCTTCACCGTCTTCTACTATGAGAGCGATGCGGACACGGCCACGGCCCTCACGCCAGCCTGGATGGAGAACCCCTACATCAAGGTGGACACGGTGGCCGCGGAGCATCTCACCCGGAAGCGCCCTGGGGCCGAGGCCACCGGGAAGGTGAATGTCAAGACGCTGCGTCTGGGACCGCTCAGCAAGGCTGGCTTCTACCTGGCCTTCCAGGACCAGGGTGCCTGCATGGCCCTGCTATCCCTGCACCTCTTCTACAAAAAGTGCGCCCAGCTGACTGTGAACCTGACTCGATTCCCGGAGACTGTGCCTCGGGAGCTGGTTGTGCCCGTGGCCGGTAGCTGCGTGGTGGATGCCGTCCCCGCCCCTGGCCCCAGCCCCAGCCTCTACTGCCGTGAGGATGGCCAGTGGGCCGAACAGCCGGTCACGGGCTGCAGCTGTGCTCCGGGGTTCGAGGCAGCTGAGGGGAACACCAAGTGCCGAGCCTGTGCCCAGGGCACCTTCAAGCCCCTGTCAGGAGAAGGGTCCTGCCAGCCATGCCCAGCCAATAGCCACTCTAACACCATTGGATCAGCCGTCTGCCAGTGCCGCGTCGGGTACTTCCGGGCACGCACAGACCCCCGGGGTGCACCCTGCACCACCCCTCCTTCGGCTCCGCGGAGCGTGGTTTCCCGCCTGAACGGCTCCTCCCTGCACCTGGAATGGAGTGCCCCCCTGGAGTCTGGTGGCCGAGAGGACCTCACCTACGCCCTCCGCTGCCGGGAGTGCCGACCCGGAGGCTCCTGTGCGCCCTGCGGGGGAGACCTGACTTTTGACCCCGGCCCCCGGGACCTGGTGGAGCCCTGGGTGGTGGTTCGAGGGCTACGTCCGGACTTCACCTATACCTTTGAGGTCACTGCATTGAACGGGGTATCCTCCTTAGCCACGGGGCCCGTCCCATTTGAGCCTGTCAATGTCACCACTGACCGAGAGGTACCTCCTGCAGTGTCTGACATCCGGGTGACGCGGTCCTCACCCAGCAGCTTGAGCCTGGCCTGGGCTGTTCCCCGGGCACCCAGTGGGGCGTGGCTGGACTACGAGGTCAAATACCATGAGAAGGGCGCCGAGGGTCCCAGCAGCGTGCGGTTCCTGAAGACGTCAGAAAACCGGGCAGAGCTGCGGGGGCTGAAGCGGGGAGCCAGCTACCTGGTGCAGGTACGGGCGCGCTCTGAGGCCGGCTACGGGCCCTTCGGCCAGGAACATCACAGCCAGACCCAACTGGATGAGAGCGAGGGCTGGCGGGAGCAGCTGGCCCTGATTGCGGGCACGGCAGTCGTGGGTGTGGTCCTGGTCCTGGTGGTCATTGTGGTCGCAGTTCTCTGCCTCAGGAAGCAGAGCAATGGGAGAGAAGCAGAATATTCGGACAAACACGGACAGTATCTCATCGGACATGGTACTAAGGTCTACATCGACCCCTTCACTTATGAAGACCCTAATGAGGCTGTGAGGGAATTTGCAAAAGAGATCGATGTCTCCTACGTCAAGATTGAAGAGGTGATTGGTGCAGGTGAGTTTGGCGAGGTGTGCCGGGGGCGGCTCAAGGCCCCAGGGAAGAAGGAGAGCTGTGTGGCAATCAAGACCCTGAAGGGTGGCTACACGGAGCGGCAGCGGCGTGAGTTTCTGAGCGAGGCCTCCATCATGGGCCAGTTCGAGCACCCCAATATCATCCGCCTGGAGGGCGTGGTCACCAACAGCATGCCCGTCATGATTCTCACAGAGTTCATGGAGAACGGCGCCCTGGACTCCTTCCTGCGGCTAAACGACGGACAGTTCACAGTCATCCAGCTCGTGGGCATGCTGCGGGGCATCGCCTCGGGCATGCGGTACCTTGCCGAGATGAGCTACGTCCACCGAGACCTGGCTGCTCGCAACATCCTAGTCAACAGCAACCTCGTCTGCAAAGTGTCTGACTTTGGCCTTTCCCGATTCCTGGAGGAGAACTCTTCCGATCCCACCTACACGAGCTCCCTGGGAGGAAAGATTCCCATCCGATGGACTGCCCCGGAGGCCATTGCCTTCCGGAAGTTCACTTCCGCCAGTGATGCCTGGAGTTACGGGATTGTGATGTGGGAGGTGATGTCATTTGGGGAGAGGCCGTACTGGGACATGAGCAATCAGGACGTGATCAATGCCATTGAACAGGACTACCGGCTGCCCCCGCCCCCAGACTGTCCCACCTCCCTCCACCAGCTCATGCTGGACTGTTGGCAGAAAGACCGGAATGCCCGGCCCCGCTTCCCCCAGGTGGTCAGCGCCCTGGACAAGATGATCCGGAACCCCGCCAGCCTCAAAATCGTGGCCCGGGAGAATGGCGGGGCCTCACACCCTCTCCTGGACCAGCGGCAGCCTCACTACTCAGCTTTTGGCTCTGTGGGCGAGTGGCTTCGGGCCATCAAAATGGGAAGATACGAAGAAAGTTTCGCAGCCGCTGGCTTTGGCTCCTTCGAGCTGGTCAGCCAGATCTCTGCTGAGGACCTGCTCCGAATCGGAGTCACTCTGGCGGGACACCAGAAGAAAATCTTGGCCAGTGTCCAGCACATGAAGTCCCAGGCCAAGCCGGGAACCCCGGGTGGGACAGGAGGACCGGCCCCGCAGTACTGACCTGCAGGAACTCCCCACCCCAGGGACACCGCCTCCCCATTTTCCGGGGCAGAGTGGGGACTCACAGAGGCCCCCAGCCCTGTGCCCCGCTGGATTGCACTTTGAGCCCGTGGGGTGAGGAGTTGGCAATTTGGAGAGACAGGATTTGGGGGTTCTGCCATAATAGGAGGGGAAAATCACCCCCCAGCCACCTCGGGGAACTCCAGACCAAGGGTGAGGGCGCCTTTCCCTCAGGACTGGGTGTGACCAGAGGAAAAGGAAGTGCCCAACATCTCCCAGCCTCCCCAGGTGCCCCCCTCACCTTGATGGGTGCGTTCCCGCAGACCAAAGAGAGTGTGACTCCCTTGCCAGCTCCAGAGTGGGGGGGCTGTCCCAGGGGGCAAGAAGGGGTGTCAGGGCCCAGTGACAAAATCATTGGGGTTTGTAGTCCCAACTTGCTGCTGTCACCACCAAACTCAATCATTTTTTTCCCTTGTAAATGCCCCTCCCCCAGCTGCTGCCTTCATATTGAAGGTTTTTGAGTTTTGTTTTTGGTCTTAATTTTTCTCCCCGTTCCCTTTTTGTTTCTTCGTTTTGTTTTTCTACCGTCCTTGTCATAACTTTGTGTTGGAGGGAACCTGTTTCACTATGGCCTCCTTTGCCCAAGTTGAAACAGGGGCCCATCATCATGTCTGTTTCCAGAACAGTGCCTTGGTCATCCCACATCCCCGGACCCCGCCTGGGACCCCCAAGCTGTGTCCTATGAAGGGGTGTGGGGTGAGGTAGTGAAAAGGGCGGTAGTTGGTGGTGGAACCCAGAAACGGACGCCGGTGCTTGGAGGGGTTCTTAAATTATATTTAAAAAAGTAACTTTTTGTATAAATAAAAGAAAATGGGACGTGTCCCAGCTCCAGGGGT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 45094, GC content failed 1826, low tm 9190, high tm 26071, high any compl 22, high end compl 51, high repeat similarity 52, long poly-x seq 138, ok 7744
PRIMER_RIGHT_EXPLAIN=considered 45319, GC content failed 2143, low tm 9281, high tm 25904, high any compl 1, high end compl 12, high repeat similarity 9, long poly-x seq 107, ok 7862
PRIMER_PAIR_EXPLAIN=considered 1444, unacceptable product size 1370, high end compl 8, ok 66
PRIMER_PAIR_PENALTY=0.1136
PRIMER_LEFT_PENALTY=0.043236
PRIMER_RIGHT_PENALTY=0.070333
PRIMER_LEFT_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_SEQUENCE=GTGATTTTCCCCTCCTATTATGG
PRIMER_LEFT=2840,23
PRIMER_RIGHT=3228,23
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=389
PRIMER_PAIR_PENALTY_1=0.1136
PRIMER_LEFT_1_PENALTY=0.043236
PRIMER_RIGHT_1_PENALTY=0.070333
PRIMER_LEFT_1_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_1_SEQUENCE=GGTGATTTTCCCCTCCTATTATG
PRIMER_LEFT_1=2840,23
PRIMER_RIGHT_1=3229,23
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.930
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=390
PRIMER_PAIR_PENALTY_2=0.1230
PRIMER_LEFT_2_PENALTY=0.043236
PRIMER_RIGHT_2_PENALTY=0.079770
PRIMER_LEFT_2_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_2_SEQUENCE=ATGACAAGGACGGTAGAAAAACA
PRIMER_LEFT_2=2840,23
PRIMER_RIGHT_2=3645,23
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=806
PRIMER_PAIR_PENALTY_3=0.1501
PRIMER_LEFT_3_PENALTY=0.070333
PRIMER_RIGHT_3_PENALTY=0.079770
PRIMER_LEFT_3_SEQUENCE=CATAATAGGAGGGGAAAATCACC
PRIMER_RIGHT_3_SEQUENCE=ATGACAAGGACGGTAGAAAAACA
PRIMER_LEFT_3=3207,23
PRIMER_RIGHT_3=3645,23
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=439
PRIMER_PAIR_PENALTY_4=0.1789
PRIMER_LEFT_4_PENALTY=0.043236
PRIMER_RIGHT_4_PENALTY=0.135712
PRIMER_LEFT_4_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_4_SEQUENCE=AAAAATGATTGAGTTTGGTGGTG
PRIMER_LEFT_4=2840,23
PRIMER_RIGHT_4=3515,23
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.136
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=676
PRIMER_PAIR_PENALTY_5=0.1876
PRIMER_LEFT_5_PENALTY=0.043236
PRIMER_RIGHT_5_PENALTY=0.144318
PRIMER_LEFT_5_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_5_SEQUENCE=AAATGATTGAGTTTGGTGGTGAC
PRIMER_LEFT_5=2840,23
PRIMER_RIGHT_5=3513,23
PRIMER_LEFT_5_TM=59.957
PRIMER_RIGHT_5_TM=60.144
PRIMER_LEFT_5_GC_PERCENT=34.783
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.1000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=674
PRIMER_PAIR_PENALTY_6=0.1948
PRIMER_LEFT_6_PENALTY=0.094334
PRIMER_RIGHT_6_PENALTY=0.100425
PRIMER_LEFT_6_SEQUENCE=ATAGCCACTCTAACACCATTGGA
PRIMER_RIGHT_6_SEQUENCE=CATAAGTGAAGGGGTCGATGTAG
PRIMER_LEFT_6=956,23
PRIMER_RIGHT_6=1873,23
PRIMER_LEFT_6_TM=59.906
PRIMER_RIGHT_6_TM=59.900
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=5.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=918
PRIMER_PAIR_PENALTY_7=0.2155
PRIMER_LEFT_7_PENALTY=0.135712
PRIMER_RIGHT_7_PENALTY=0.079770
PRIMER_LEFT_7_SEQUENCE=CACCACCAAACTCAATCATTTTT
PRIMER_RIGHT_7_SEQUENCE=ATGACAAGGACGGTAGAAAAACA
PRIMER_LEFT_7=3493,23
PRIMER_RIGHT_7=3645,23
PRIMER_LEFT_7_TM=60.136
PRIMER_RIGHT_7_TM=59.920
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=153
PRIMER_PAIR_PENALTY_8=0.2195
PRIMER_LEFT_8_PENALTY=0.149159
PRIMER_RIGHT_8_PENALTY=0.070333
PRIMER_LEFT_8_SEQUENCE=CATCAAAATGGGAAGATACGAAG
PRIMER_RIGHT_8_SEQUENCE=GTGATTTTCCCCTCCTATTATGG
PRIMER_LEFT_8=2838,23
PRIMER_RIGHT_8=3228,23
PRIMER_LEFT_8_TM=59.851
PRIMER_RIGHT_8_TM=59.930
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_8_END_STABILITY=8.7000
PRIMER_RIGHT_8_END_STABILITY=7.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=391
PRIMER_PAIR_PENALTY_9=0.2241
PRIMER_LEFT_9_PENALTY=0.144318
PRIMER_RIGHT_9_PENALTY=0.079770
PRIMER_LEFT_9_SEQUENCE=GTCACCACCAAACTCAATCATTT
PRIMER_RIGHT_9_SEQUENCE=ATGACAAGGACGGTAGAAAAACA
PRIMER_LEFT_9=3491,23
PRIMER_RIGHT_9=3645,23
PRIMER_LEFT_9_TM=60.144
PRIMER_RIGHT_9_TM=59.920
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=155
PRIMER_PAIR_PENALTY_10=0.2244
PRIMER_LEFT_10_PENALTY=0.094334
PRIMER_RIGHT_10_PENALTY=0.130037
PRIMER_LEFT_10_SEQUENCE=ATAGCCACTCTAACACCATTGGA
PRIMER_RIGHT_10_SEQUENCE=TGTCCGAATATTCTGCTTCTCTC
PRIMER_LEFT_10=956,23
PRIMER_RIGHT_10=1813,23
PRIMER_LEFT_10_TM=59.906
PRIMER_RIGHT_10_TM=59.870
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.5000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=858
PRIMER_PAIR_PENALTY_11=0.2289
PRIMER_LEFT_11_PENALTY=0.149159
PRIMER_RIGHT_11_PENALTY=0.079770
PRIMER_LEFT_11_SEQUENCE=CATCAAAATGGGAAGATACGAAG
PRIMER_RIGHT_11_SEQUENCE=ATGACAAGGACGGTAGAAAAACA
PRIMER_LEFT_11=2838,23
PRIMER_RIGHT_11=3645,23
PRIMER_LEFT_11_TM=59.851
PRIMER_RIGHT_11_TM=59.920
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_11_END_STABILITY=8.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=808
PRIMER_PAIR_PENALTY_12=0.2564
PRIMER_LEFT_12_PENALTY=0.213146
PRIMER_RIGHT_12_PENALTY=0.043236
PRIMER_LEFT_12_SEQUENCE=AGATCGATGTCTCCTACGTCAAG
PRIMER_RIGHT_12_SEQUENCE=TTCTTCGTATCTTCCCATTTTGA
PRIMER_LEFT_12=1910,23
PRIMER_RIGHT_12=2862,23
PRIMER_LEFT_12_TM=59.787
PRIMER_RIGHT_12_TM=59.957
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.3000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=953
PRIMER_PAIR_PENALTY_13=0.2849
PRIMER_LEFT_13_PENALTY=0.149159
PRIMER_RIGHT_13_PENALTY=0.135712
PRIMER_LEFT_13_SEQUENCE=CATCAAAATGGGAAGATACGAAG
PRIMER_RIGHT_13_SEQUENCE=AAAAATGATTGAGTTTGGTGGTG
PRIMER_LEFT_13=2838,23
PRIMER_RIGHT_13=3515,23
PRIMER_LEFT_13_TM=59.851
PRIMER_RIGHT_13_TM=60.136
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=8.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=678
PRIMER_PAIR_PENALTY_14=0.2935
PRIMER_LEFT_14_PENALTY=0.149159
PRIMER_RIGHT_14_PENALTY=0.144318
PRIMER_LEFT_14_SEQUENCE=CATCAAAATGGGAAGATACGAAG
PRIMER_RIGHT_14_SEQUENCE=AAATGATTGAGTTTGGTGGTGAC
PRIMER_LEFT_14=2838,23
PRIMER_RIGHT_14=3513,23
PRIMER_LEFT_14_TM=59.851
PRIMER_RIGHT_14_TM=60.144
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.7000
PRIMER_RIGHT_14_END_STABILITY=6.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=676
PRIMER_PAIR_PENALTY_15=0.3124
PRIMER_LEFT_15_PENALTY=0.043236
PRIMER_RIGHT_15_PENALTY=0.269187
PRIMER_LEFT_15_SEQUENCE=TCAAAATGGGAAGATACGAAGAA
PRIMER_RIGHT_15_SEQUENCE=GGGGAGAAAAATTAAGACCAAAA
PRIMER_LEFT_15=2840,23
PRIMER_RIGHT_15=3599,23
PRIMER_LEFT_15_TM=59.957
PRIMER_RIGHT_15_TM=59.731
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=2.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=760
PRIMER_PAIR_PENALTY_16=0.3186
PRIMER_LEFT_16_PENALTY=0.094334
PRIMER_RIGHT_16_PENALTY=0.224301
PRIMER_LEFT_16_SEQUENCE=ATAGCCACTCTAACACCATTGGA
PRIMER_RIGHT_16_SEQUENCE=CATGGTATTTGACCTCGTAGTCC
PRIMER_LEFT_16=956,23
PRIMER_RIGHT_16=1501,23
PRIMER_LEFT_16_TM=59.906
PRIMER_RIGHT_16_TM=59.776
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=546
PRIMER_PAIR_PENALTY_17=0.3247
PRIMER_LEFT_17_PENALTY=0.224301
PRIMER_RIGHT_17_PENALTY=0.100425
PRIMER_LEFT_17_SEQUENCE=TGGACTACGAGGTCAAATACCAT
PRIMER_RIGHT_17_SEQUENCE=CATAAGTGAAGGGGTCGATGTAG
PRIMER_LEFT_17=1478,23
PRIMER_RIGHT_17=1873,23
PRIMER_LEFT_17_TM=59.776
PRIMER_RIGHT_17_TM=59.900
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.8000
PRIMER_RIGHT_17_END_STABILITY=5.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=396
PRIMER_PAIR_PENALTY_18=0.3395
PRIMER_LEFT_18_PENALTY=0.070333
PRIMER_RIGHT_18_PENALTY=0.269187
PRIMER_LEFT_18_SEQUENCE=CATAATAGGAGGGGAAAATCACC
PRIMER_RIGHT_18_SEQUENCE=GGGGAGAAAAATTAAGACCAAAA
PRIMER_LEFT_18=3207,23
PRIMER_RIGHT_18=3599,23
PRIMER_LEFT_18_TM=59.930
PRIMER_RIGHT_18_TM=59.731
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER7B Nonautonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=7.6000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=393
PRIMER_PAIR_PENALTY_19=0.3405
PRIMER_LEFT_19_PENALTY=0.210476
PRIMER_RIGHT_19_PENALTY=0.130037
PRIMER_LEFT_19_SEQUENCE=GTCCGGACTTCACCTATACCTTT
PRIMER_RIGHT_19_SEQUENCE=TGTCCGAATATTCTGCTTCTCTC
PRIMER_LEFT_19=1274,23
PRIMER_RIGHT_19=1813,23
PRIMER_LEFT_19_TM=59.790
PRIMER_RIGHT_19_TM=59.870
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MER39 a consensus
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=540
=