PRIMER_SEQUENCE_ID=NM_004445
SEQUENCE=CGGAGGGGGCGGGCCGGGCTGCGTTCGCTCCAGCCGCGGCTCTACAGCAGCGGGCGGCGGGACCCGGGACCCAGCTTGGCGACGGCGATTCTCGACGCGGGCCCCCAGGATTCTCCCGGCGCCCCACCTCTGGAGCAGCCCCTGCCGCCAGCGTCAGGTCCACCCCGGAATCCCAGGGACTCTCGGCGCCGAACGGACCCGGGCCGGTGCAACGGGGTCCCCGGACTGGAGAAGACGCGGGTGGCACCGTGCGAGCTCCAGGAGCCCCGGGTCCACTGCGAGGCCTCGGGGGGCGCAGACCTGCAGAGACTGCGGCCAACGGGAAGAAATAAAGGGATTATAGTCCACCCAATTCACAGACTTCTGAGACTCAGACACGAGGAGAGATAGAGAACCGCCAATCTCTAGATCAACAAGCAAAGGAGGTGCCAAGCCTGTTTGTCTTCATTGTGACACTGGAGTCTAGATGCTGGGAAGTCCAAGATCAGGGTGCCGGCATGGTCAGTTCCTGGCGAAGCCTCTCTTCTAGGTTTCAGACTGCCCTCTTCTTTGTTGTGTCCTCGAATGGCAGAAAAAGGGGTGGCTGTTGGAGGAAGGGAGGAGAGTAAATGAAGAGAAAGAACTGGAATAACCCCTTGCAGAAAAAAAAAAAAAGGGAAGCTTAGCTGTACACCCTGAGTCTTGCAAAAGCTGCAGCCCCACCCAGGAGCAGGGTGGTGGCTGGGGCGATGGTGGACGCCCTGAAGATGTCCCATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGGCATGGTGTGTAGCCTATGGGTGCTGCTCCTGGTGTCTTCAGTTCTGGCTCTGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGGCTGGCTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCAGCGACGCCTGACTCGGACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTCCAGGCACCGGGCAGGACAATTGGTTGCAGACACACTTTGTGGAGCGGCGCGGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTCCAGCCTGGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACTACCGTCAGGCTGAGGAGCCCGACAGCCCTGACAGCGTTTCCTCCTGGCACCTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCTTTCCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGGGACCCCACGGGGCTGGGCAGCGGGCTGGACTGCAACTGAACGTCAAAGAGCGGAGCTTTGGGCCTCTCACCCAACGCGGCTTCTACGTGGCCTTCCAGGACACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCTGCCCTGCCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGTGGGGCTGGGGGGGCCTCCCTGGTGGCAGCTGTGGGCACCTGTGTGGCTCATGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGCAGCCCCCCCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGCTGCCGCTGCCAGCCTGGATACCAACCAGCACGAGGAGACAAGGCCTGCCAAGCCTGCCCACGGGGGCTCTATAAGTCTTCTGCTGGGAATGCTCCCTGCTCACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGCCCCTGCCTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCCCTGCACTGGTCCTCCATCGGCTCCCCAGGAGCTTTGGTTTGAGGTGCAAGGCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGGGGGTCGAGGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGAACCTGCCAGCGGTGGTGGGGGCACTTGTCACCGCTGCAGGGATGAGGTCCACTTCGACCCTCGCCAGAGAGGCCTGACTGAGAGCCGAGTGTTAGTGGGGGGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGGGGTGTCTGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCAGCACCAGCCATGAAGTGCCCTCTGCTGTCCCTGTGGTGCACCAGGTGAGCCGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACCAGACCAATGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACGAATCCCACTCCTTCACCCTGACCAGCGAGACCAACACTGCCACCGTGACACAGCTGAGCCCTGGCCACATCTATGGTTTCCAGGTGCGGGCCCGGACTGCTGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTCAAGGGGAGCTGTCTTCCCAGCTTCCGGAAAGACTCTCCTTGGTGATCGGCTCCATCCTGGGGGCTTTGGCCTTCCTCCTGCTGGCAGCCATCACCGTGCTGGCGGTCGTCTTCCAGCGGAAGCGGCGTGGGACTGGCTACACGGAGCAGCTGCAGCAATACAGCAGCCCAGGACTCGGGGTGAAGTATTACATCGACCCCTCCACCTACGAGGACCCCTGTCAGGCCATCCGAGAACTTGCCCGGGAAGTCGATCCTGCTTATATCAAGATTGAGGAGGTCATTGGGACAGGCTCTTTTGGAGAAGTGCGCCAGGGCCGCCTGCAGCCACGGGGACGGAGGGAGCAGACTGTGGCCATCCAGGCCCTGTGGGCCGGGGGCGCCGAAAGCCTGCAGATGACCTTCCTGGGCCGGGCCGCAGTGCTGGGTCAGTTCCAGCACCCCAACATCCTGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCATGGTGCTGACGGAGTTCATGGAGCTTGGCCCCCTGGACAGCTTCCTCAGGCAGCGGGAGGGCCAGTTCAGCAGCCTGCAGCTGGTGGCCATGCAGCGGGGAGTGGCTGCTGCCATGCAGTACCTGTCCAGCTTTGCCTTCGTCCATCGCTCGCTGTCTGCCCACAGCGTGCTGGTGAATAGCCACTTGGTGTGCAAGGTGGCCCGTCTTGGCCACAGTCCTCAGGGCCCAAGTTGTTTGCTTCGCTGGGCAGCCCCAGAGGTCATTGCACATGGAAAGCATACAACATCCAGTGATGTCTGGAGCTTTGGGATACTCATGTGGGAAGTGATGAGTTATGGAGAACGGCCTTACTGGGACATGAGTGAGCAGGAGGTACTAAATGCAATAGAGCAGGAGTTCCGGCTGCCCCCGCCTCCAGGCTGTCCTCCTGGATTACATCTACTTATGTTGGACACTTGGCAGAAGGACCGTGCCCGGCGGCCTCATTTTGACCAGCTGGTGGCTGCATTTGACAAGATGATCCGCAAGCCAGATACCCTGCAGGCTGGCGGGGACCCAGGGGAAAGGCCTTCCCAGGCCCTTCTGACCCCTGTGGCCCTGGACTTTCCTTGTCTGGACTCACCCCAGGCCTGGCTTTCAGCCATTGGACTGGAGTGCTACCAGGACAACTTCTCCAAGTTTGGCCTCTGTACCTTCAGTGATGTGGCTCAGCTCAGCCTAGAAGACCTGCCTGCCCTGGGCATCACCCTGGCTGGCCACCAGAAGAAGCTGCTGCACCACATCCAGCTCCTTCAGCAACACCTGAGGCAGCAGGGCTCAGTGGAGGTCTGAGAATGACGATACCCGTGACTCAGCCCTGGACACTGGTCCGAGAAGGGACATGTGGGACGTGAGCCGGGCTCCAACAGCCTCTGTGAGAGATGCCCCACACCAAACCCAACCCTCCCGATGGCTGCATTCCCTGGTCCTCCGCCTCTCCACCAGCCCCCTCCTCATTAAAGGGAAAGAAGGGAATTTGCAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 45878, GC content failed 2110, low tm 9661, high tm 25835, high any compl 9, high end compl 48, high repeat similarity 32, long poly-x seq 151, ok 8032
PRIMER_RIGHT_EXPLAIN=considered 45910, GC content failed 1858, low tm 9831, high tm 25839, high any compl 2, high end compl 12, high repeat similarity 12, long poly-x seq 144, ok 8212
PRIMER_PAIR_EXPLAIN=considered 4263, unacceptable product size 4188, high end compl 4, ok 71
PRIMER_PAIR_PENALTY=0.2849
PRIMER_LEFT_PENALTY=0.085505
PRIMER_RIGHT_PENALTY=0.199411
PRIMER_LEFT_SEQUENCE=TAGTCCACCCAATTCACAGACTT
PRIMER_RIGHT_SEQUENCE=ATACCTCTTCCAGAGCCAGAACT
PRIMER_LEFT=340,23
PRIMER_RIGHT=858,23
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.801
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=519
PRIMER_PAIR_PENALTY_1=0.2998
PRIMER_LEFT_1_PENALTY=0.100425
PRIMER_RIGHT_1_PENALTY=0.199411
PRIMER_LEFT_1_SEQUENCE=TCTAGATCAACAAGCAAAGGAGG
PRIMER_RIGHT_1_SEQUENCE=ATACCTCTTCCAGAGCCAGAACT
PRIMER_LEFT_1=403,23
PRIMER_RIGHT_1=858,23
PRIMER_LEFT_1_TM=59.900
PRIMER_RIGHT_1_TM=59.801
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=456
PRIMER_PAIR_PENALTY_2=0.3030
PRIMER_LEFT_2_PENALTY=0.085505
PRIMER_RIGHT_2_PENALTY=0.217466
PRIMER_LEFT_2_SEQUENCE=TAGTCCACCCAATTCACAGACTT
PRIMER_RIGHT_2_SEQUENCE=CAAGGGGTTATTCCAGTTCTTTC
PRIMER_LEFT_2=340,23
PRIMER_RIGHT_2=637,23
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.217
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.3179
PRIMER_LEFT_3_PENALTY=0.100425
PRIMER_RIGHT_3_PENALTY=0.217466
PRIMER_LEFT_3_SEQUENCE=TCTAGATCAACAAGCAAAGGAGG
PRIMER_RIGHT_3_SEQUENCE=CAAGGGGTTATTCCAGTTCTTTC
PRIMER_LEFT_3=403,23
PRIMER_RIGHT_3=637,23
PRIMER_LEFT_3_TM=59.900
PRIMER_RIGHT_3_TM=60.217
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.3654
PRIMER_LEFT_4_PENALTY=0.085505
PRIMER_RIGHT_4_PENALTY=0.279943
PRIMER_LEFT_4_SEQUENCE=TAGTCCACCCAATTCACAGACTT
PRIMER_RIGHT_4_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_4=340,23
PRIMER_RIGHT_4=1152,23
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.280
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=813
PRIMER_PAIR_PENALTY_5=0.3804
PRIMER_LEFT_5_PENALTY=0.100425
PRIMER_RIGHT_5_PENALTY=0.279943
PRIMER_LEFT_5_SEQUENCE=TCTAGATCAACAAGCAAAGGAGG
PRIMER_RIGHT_5_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_5=403,23
PRIMER_RIGHT_5=1152,23
PRIMER_LEFT_5_TM=59.900
PRIMER_RIGHT_5_TM=60.280
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=9.4000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=750
PRIMER_PAIR_PENALTY_6=0.4039
PRIMER_LEFT_6_PENALTY=0.279943
PRIMER_RIGHT_6_PENALTY=0.123924
PRIMER_LEFT_6_SEQUENCE=GACCTTCACCCTTTACTACCGTC
PRIMER_RIGHT_6_SEQUENCE=ACTAACACTCGGCTCTCAGTCAG
PRIMER_LEFT_6=1130,23
PRIMER_RIGHT_6=2092,23
PRIMER_LEFT_6_TM=60.280
PRIMER_RIGHT_6_TM=60.124
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=52.174
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=963
PRIMER_PAIR_PENALTY_7=0.4169
PRIMER_LEFT_7_PENALTY=0.217466
PRIMER_RIGHT_7_PENALTY=0.199411
PRIMER_LEFT_7_SEQUENCE=GAAAGAACTGGAATAACCCCTTG
PRIMER_RIGHT_7_SEQUENCE=ATACCTCTTCCAGAGCCAGAACT
PRIMER_LEFT_7=615,23
PRIMER_RIGHT_7=858,23
PRIMER_LEFT_7_TM=60.217
PRIMER_RIGHT_7_TM=59.801
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=244
PRIMER_PAIR_PENALTY_8=0.4219
PRIMER_LEFT_8_PENALTY=0.141958
PRIMER_RIGHT_8_PENALTY=0.279943
PRIMER_LEFT_8_SEQUENCE=GTTCTGGCTCTGGAAGAGGTATT
PRIMER_RIGHT_8_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_8=837,23
PRIMER_RIGHT_8=1152,23
PRIMER_LEFT_8_TM=60.142
PRIMER_RIGHT_8_TM=60.280
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, MER39 a consensus
PRIMER_LEFT_8_END_STABILITY=5.6000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=316
PRIMER_PAIR_PENALTY_9=0.4352
PRIMER_LEFT_9_PENALTY=0.235765
PRIMER_RIGHT_9_PENALTY=0.199411
PRIMER_LEFT_9_SEQUENCE=GAGAGATAGAGAACCGCCAATCT
PRIMER_RIGHT_9_SEQUENCE=ATACCTCTTCCAGAGCCAGAACT
PRIMER_LEFT_9=381,23
PRIMER_RIGHT_9=858,23
PRIMER_LEFT_9_TM=60.236
PRIMER_RIGHT_9_TM=59.801
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=478
PRIMER_PAIR_PENALTY_10=0.4532
PRIMER_LEFT_10_PENALTY=0.235765
PRIMER_RIGHT_10_PENALTY=0.217466
PRIMER_LEFT_10_SEQUENCE=GAGAGATAGAGAACCGCCAATCT
PRIMER_RIGHT_10_SEQUENCE=CAAGGGGTTATTCCAGTTCTTTC
PRIMER_LEFT_10=381,23
PRIMER_RIGHT_10=637,23
PRIMER_LEFT_10_TM=60.236
PRIMER_RIGHT_10_TM=60.217
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=257
PRIMER_PAIR_PENALTY_11=0.4794
PRIMER_LEFT_11_PENALTY=0.199411
PRIMER_RIGHT_11_PENALTY=0.279943
PRIMER_LEFT_11_SEQUENCE=AGTTCTGGCTCTGGAAGAGGTAT
PRIMER_RIGHT_11_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_11=836,23
PRIMER_RIGHT_11=1152,23
PRIMER_LEFT_11_TM=59.801
PRIMER_RIGHT_11_TM=60.280
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.8000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=317
PRIMER_PAIR_PENALTY_12=0.4819
PRIMER_LEFT_12_PENALTY=0.085505
PRIMER_RIGHT_12_PENALTY=0.396441
PRIMER_LEFT_12_SEQUENCE=TAGTCCACCCAATTCACAGACTT
PRIMER_RIGHT_12_SEQUENCE=CGGTAGTAAAGGGTGAAGGTCTC
PRIMER_LEFT_12=340,23
PRIMER_RIGHT_12=1150,23
PRIMER_LEFT_12_TM=59.914
PRIMER_RIGHT_12_TM=60.396
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.4000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=811
PRIMER_PAIR_PENALTY_13=0.4969
PRIMER_LEFT_13_PENALTY=0.100425
PRIMER_RIGHT_13_PENALTY=0.396441
PRIMER_LEFT_13_SEQUENCE=TCTAGATCAACAAGCAAAGGAGG
PRIMER_RIGHT_13_SEQUENCE=CGGTAGTAAAGGGTGAAGGTCTC
PRIMER_LEFT_13=403,23
PRIMER_RIGHT_13=1150,23
PRIMER_LEFT_13_TM=59.900
PRIMER_RIGHT_13_TM=60.396
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_13_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.4000
PRIMER_RIGHT_13_END_STABILITY=6.1000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=748
PRIMER_PAIR_PENALTY_14=0.4974
PRIMER_LEFT_14_PENALTY=0.217466
PRIMER_RIGHT_14_PENALTY=0.279943
PRIMER_LEFT_14_SEQUENCE=GAAAGAACTGGAATAACCCCTTG
PRIMER_RIGHT_14_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_14=615,23
PRIMER_RIGHT_14=1152,23
PRIMER_LEFT_14_TM=60.217
PRIMER_RIGHT_14_TM=60.280
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=538
PRIMER_PAIR_PENALTY_15=0.5157
PRIMER_LEFT_15_PENALTY=0.235765
PRIMER_RIGHT_15_PENALTY=0.279943
PRIMER_LEFT_15_SEQUENCE=GAGAGATAGAGAACCGCCAATCT
PRIMER_RIGHT_15_SEQUENCE=GACGGTAGTAAAGGGTGAAGGTC
PRIMER_LEFT_15=381,23
PRIMER_RIGHT_15=1152,23
PRIMER_LEFT_15_TM=60.236
PRIMER_RIGHT_15_TM=60.280
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MER39 a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, MER4C a consensus
PRIMER_LEFT_15_END_STABILITY=6.6000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=772
PRIMER_PAIR_PENALTY_16=0.5160
PRIMER_LEFT_16_PENALTY=0.478849
PRIMER_RIGHT_16_PENALTY=0.037129
PRIMER_LEFT_16_SEQUENCE=AAGTATTACATCGACCCCTCCAC
PRIMER_RIGHT_16_SEQUENCE=AGTAAGGCCGTTCTCCATAACTC
PRIMER_LEFT_16=2679,23
PRIMER_RIGHT_16=3339,23
PRIMER_LEFT_16_TM=60.479
PRIMER_RIGHT_16_TM=60.037
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=10.00, MER4B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=661
PRIMER_PAIR_PENALTY_17=0.5204
PRIMER_LEFT_17_PENALTY=0.396441
PRIMER_RIGHT_17_PENALTY=0.123924
PRIMER_LEFT_17_SEQUENCE=GAGACCTTCACCCTTTACTACCG
PRIMER_RIGHT_17_SEQUENCE=ACTAACACTCGGCTCTCAGTCAG
PRIMER_LEFT_17=1128,23
PRIMER_RIGHT_17=2092,23
PRIMER_LEFT_17_TM=60.396
PRIMER_RIGHT_17_TM=60.124
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=965
PRIMER_PAIR_PENALTY_18=0.5329
PRIMER_LEFT_18_PENALTY=0.085505
PRIMER_RIGHT_18_PENALTY=0.447437
PRIMER_LEFT_18_SEQUENCE=TAGTCCACCCAATTCACAGACTT
PRIMER_RIGHT_18_SEQUENCE=GTGTCACAATGAAGACAAACAGG
PRIMER_LEFT_18=340,23
PRIMER_RIGHT_18=455,23
PRIMER_LEFT_18_TM=59.914
PRIMER_RIGHT_18_TM=59.553
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=116
PRIMER_PAIR_PENALTY_19=0.5384
PRIMER_LEFT_19_PENALTY=0.141958
PRIMER_RIGHT_19_PENALTY=0.396441
PRIMER_LEFT_19_SEQUENCE=GTTCTGGCTCTGGAAGAGGTATT
PRIMER_RIGHT_19_SEQUENCE=CGGTAGTAAAGGGTGAAGGTCTC
PRIMER_LEFT_19=837,23
PRIMER_RIGHT_19=1150,23
PRIMER_LEFT_19_TM=60.142
PRIMER_RIGHT_19_TM=60.396
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=314
=