PRIMER_SEQUENCE_ID=NM_004448
SEQUENCE=AATTCTCGAGCTCGTCGACCGGTCGACGAGCTCGAGGGTCGACGAGCTCGAGGGCGCGCGCCCGGCCCCCACCCCTCGCAGCACCCCGCGCCCCGCGCCCTCCCAGCCGGGTCCAGCCGGAGCCATGGGGCCGGAGCCGCAGTGAGCACCATGGAGCTGGCGGCCTTGTGCCGCTGGGGGCTCCTCCTCGCCCTCTTGCCCCCCGGAGCCGCGAGCACCCAAGTGTGCACCGGCACAGACATGAAGCTGCGGCTCCCTGCCAGTCCCGAGACCCACCTGGACATGCTCCGCCACCTCTACCAGGGCTGCCAGGTGGTGCAGGGAAACCTGGAACTCACCTACCTGCCCACCAATGCCAGCCTGTCCTTCCTGCAGGATATCCAGGAGGTGCAGGGCTACGTGCTCATCGCTCACAACCAAGTGAGGCAGGTCCCACTGCAGAGGCTGCGGATTGTGCGAGGCACCCAGCTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAATGGAGACCCGCTGAACAATACCACCCCTGTCACAGGGGCCTCCCCAGGAGGCCTGCGGGAGCTGCAGCTTCGAAGCCTCACAGAGATCTTGAAAGGAGGGGTCTTGATCCAGCGGAACCCCCAGCTCTGCTACCAGGACACGATTTTGTGGAAGGACATCTTCCACAAGAACAACCAGCTGGCTCTCACACTGATAGACACCAACCGCTCTCGGGCCTGCCACCCCTGTTCTCCGATGTGTAAGGGCTCCCGCTGCTGGGGAGAGAGTTCTGAGGATTGTCAGAGCCTGACGCGCACTGTCTGTGCCGGTGGCTGTGCCCGCTGCAAGGGGCCACTGCCCACTGACTGCTGCCATGAGCAGTGTGCTGCCGGCTGCACGGGCCCCAAGCACTCTGACTGCCTGGCCTGCCTCCACTTCAACCACAGTGGCATCTGTGAGCTGCACTGCCCAGCCCTGGTCACCTACAACACAGACACGTTTGAGTCCATGCCCAATCCCGAGGGCCGGTATACATTCGGCGCCAGCTGTGTGACTGCCTGTCCCTACAACTACCTTTCTACGGACGTGGGATCCTGCACCCTCGTCTGCCCCCTGCACAACCAAGAGGTGACAGCAGAGGATGGAACACAGCGGTGTGAGAAGTGCAGCAAGCCCTGTGCCCGAGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTGAGGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTGCAAGAAGATCTTTGGGAGCCTGGCATTTCTGCCGGAGAGCTTTGATGGGGACCCAGCCTCCAACACTGCCCCGCTCCAGCCAGAGCAGCTCCAAGTGTTTGAGACTCTGGAAGAGATCACAGGTTACCTATACATCTCAGCATGGCCGGACAGCCTGCCTGACCTCAGCGTCTTCCAGAACCTGCAAGTAATCCGGGGACGAATTCTGCACAATGGCGCCTACTCGCTGACCCTGCAAGGGCTGGGCATCAGCTGGCTGGGGCTGCGCTCACTGAGGGAACTGGGCAGTGGACTGGCCCTCATCCACCATAACACCCACCTCTGCTTCGTGCACACGGTGCCCTGGGACCAGCTCTTTCGGAACCCGCACCAAGCTCTGCTCCACACTGCCAACCGGCCAGAGGACGAGTGTGTGGGCGAGGGCCTGGCCTGCCACCAGCTGTGCGCCCGAGGGCACTGCTGGGGTCCAGGGCCCACCCAGTGTGTCAACTGCAGCCAGTTCCTTCGGGGCCAGGAGTGCGTGGAGGAATGCCGAGTACTGCAGGGGCTCCCCAGGGAGTATGTGAATGCCAGGCACTGTTTGCCGTGCCACCCTGAGTGTCAGCCCCAGAATGGCTCAGTGACCTGTTTTGGACCGGAGGCTGACCAGTGTGTGGCCTGTGCCCACTATAAGGACCCTCCCTTCTGCGTGGCCCGCTGCCCCAGCGGTGTGAAACCTGACCTCTCCTACATGCCCATCTGGAAGTTTCCAGATGAGGAGGGCGCATGCCAGCCTTGCCCCATCAACTGCACCCACTCCTGTGTGGACCTGGATGACAAGGGCTGCCCCGCCGAGCAGAGAGCCAGCCCTCTGACGTCCATCGTCTCTGCGGTGGTTGGCATTCTGCTGGTCGTGGTCTTGGGGGTGGTCTTTGGGATCCTCATCAAGCGACGGCAGCAGAAGATCCGGAAGTACACGATGCGGAGACTGCTGCAGGAAACGGAGCTGGTGGAGCCGCTGACACCTAGCGGAGCGATGCCCAACCAGGCGCAGATGCGGATCCTGAAAGAGACGGAGCTGAGGAAGGTGAAGGTGCTTGGATCTGGCGCTTTTGGCACAGTCTACAAGGGCATCTGGATCCCTGATGGGGAGAATGTGAAAATTCCAGTGGCCATCAAAGTGTTGAGGGAAAACACATCCCCCAAAGCCAACAAAGAAATCTTAGACGAAGCATACGTGATGGCTGGTGTGGGCTCCCCATATGTCTCCCGCCTTCTGGGCATCTGCCTGACATCCACGGTGCAGCTGGTGACACAGCTTATGCCCTATGGCTGCCTCTTAGACCATGTCCGGGAAAACCGCGGACGCCTGGGCTCCCAGGACCTGCTGAACTGGTGTATGCAGATTGCCAAGGGGATGAGCTACCTGGAGGATGTGCGGCTCGTACACAGGGACTTGGCCGCTCGGAACGTGCTGGTCAAGAGTCCCAACCATGTCAAAATTACAGACTTCGGGCTGGCTCGGCTGCTGGACATTGACGAGACAGAGTACCATGCAGATGGGGGCAAGGTGCCCATCAAGTGGATGGCGCTGGAGTCCATTCTCCGCCGGCGGTTCACCCACCAGAGTGATGTGTGGAGTTATGGTGTGACTGTGTGGGAGCTGATGACTTTTGGGGCCAAACCTTACGATGGGATCCCAGCCCGGGAGATCCCTGACCTGCTGGAAAAGGGGGAGCGGCTGCCCCAGCCCCCCATCTGCACCATTGATGTCTACATGATCATGGTCAAATGTTGGATGATTGACTCTGAATGTCGGCCAAGATTCCGGGAGTTGGTGTCTGAATTCTCCCGCATGGCCAGGGACCCCCAGCGCTTTGTGGTCATCCAGAATGAGGACTTGGGCCCAGCCAGTCCCTTGGACAGCACCTTCTACCGCTCACTGCTGGAGGACGATGACATGGGGGACCTGGTGGATGCTGAGGAGTATCTGGTACCCCAGCAGGGCTTCTTCTGTCCAGACCCTGCCCCGGGCGCTGGGGGCATGGTCCACCACAGGCACCGCAGCTCATCTACCAGGAGTGGCGGTGGGGACCTGACACTAGGGCTGGAGCCCTCTGAAGAGGAGGCCCCCAGGTCTCCACTGGCACCCTCCGAAGGGGCTGGCTCCGATGTATTTGATGGTGACCTGGGAATGGGGGCAGCCAAGGGGCTGCAAAGCCTCCCCACACATGACCCCAGCCCTCTACAGCGGTACAGTGAGGACCCCACAGTACCCCTGCCCTCTGAGACTGATGGCTACGTTGCCCCCCTGACCTGCAGCCCCCAGCCTGAATATGTGAACCAGCCAGATGTTCGGCCCCAGCCCCCTTCGCCCCGAGAGGGCCCTCTGCCTGCTGCCCGACCTGCTGGTGCCACTCTGGAAAGGGCCAAGACTCTCTCCCCAGGGAAGAATGGGGTCGTCAAAGACGTTTTTGCCTTTGGGGGTGCCGTGGAGAACCCCGAGTACTTGACACCCCAGGGAGGAGCTGCCCCTCAGCCCCACCCTCCTCCTGCCTTCAGCCCAGCCTTCGACAACCTCTATTACTGGGACCAGGACCCACCAGAGCGGGGGGCTCCACCCAGCACCTTCAAAGGGACACCTACGGCAGAGAACCCAGAGTACCTGGGTCTGGACGTGCCAGTGTGAACCAGAAGGCCAAGTCCGCAGAAGCCCTGATGTGTCCTCAGGGAGCAGGGAAGGCCTGACTTCTGCTGGCATCAAGAGGTGGGAGGGCCCTCCGACCACTTCCAGGGGAACCTGCCATGCCAGGAACCTGTCCTAAGGAACCTTCCTTCCTGCTTGAGTTCCCAGATGGCTGGAAGGGGTCCAGCCTCGTTGGAAGAGGAACAGCACTGGGGAGTCTTTGTGGATTCTGAGGCCCTGCCCAATGAGACTCTAGGGTCCAGTGGATGCCACAGCCCAGCTTGGCCCTTTCCTTCCAGATCCTGGGTACTGAAAGCCTTAGGGAAGCTGGCCTGAGAGGGGAAGCGGCCCTAAGGGAGTGTCTAAGAACAAAAGCGACCCATTCAGAGACTGTCCCTGAAACCTAGTACTGCCCCCCATGAGGAAGGAACAGCAATGGTGTCAGTATCCAGGCTTTGTACAGAGTGCTTTTCTGTTTAGTTTTTACTTTTTTTGTTTTGTTTTTTTAAAGACGAAATAAAGACCCAGGGGAGAATGGGTGTTGTATGGGGAGGCAAGTGTGGGGGGTCCTTCTCCACACCCACTTTGTCCATTTGCAAATATATTTTGGAAAAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 52306, GC content failed 1769, low tm 11318, high tm 29446, high any compl 8, high end compl 48, high repeat similarity 48, long poly-x seq 122, ok 9547
PRIMER_RIGHT_EXPLAIN=considered 52131, GC content failed 1363, low tm 11573, high tm 29328, high any compl 5, high end compl 4, high repeat similarity 5, long poly-x seq 141, ok 9712
PRIMER_PAIR_EXPLAIN=considered 1370, unacceptable product size 1315, high end compl 5, ok 50
PRIMER_PAIR_PENALTY=0.0386
PRIMER_LEFT_PENALTY=0.007752
PRIMER_RIGHT_PENALTY=0.030877
PRIMER_LEFT_SEQUENCE=TTCGACAACCTCTATTACTGGGA
PRIMER_RIGHT_SEQUENCE=CTAAGGCTTTCAGTACCCAGGAT
PRIMER_LEFT=3798,23
PRIMER_RIGHT=4236,23
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=439
PRIMER_PAIR_PENALTY_1=0.0656
PRIMER_LEFT_1_PENALTY=0.007752
PRIMER_RIGHT_1_PENALTY=0.057845
PRIMER_LEFT_1_SEQUENCE=TTCGACAACCTCTATTACTGGGA
PRIMER_RIGHT_1_SEQUENCE=ATACTGACACCATTGCTGTTCCT
PRIMER_LEFT_1=3798,23
PRIMER_RIGHT_1=4362,23
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.942
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MER34 a consensus
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=565
PRIMER_PAIR_PENALTY_2=0.0670
PRIMER_LEFT_2_PENALTY=0.007752
PRIMER_RIGHT_2_PENALTY=0.059210
PRIMER_LEFT_2_SEQUENCE=TTCGACAACCTCTATTACTGGGA
PRIMER_RIGHT_2_SEQUENCE=TCGCTTTTGTTCTTAGACACTCC
PRIMER_LEFT_2=3798,23
PRIMER_RIGHT_2=4292,23
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.941
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=495
PRIMER_PAIR_PENALTY_3=0.0886
PRIMER_LEFT_3_PENALTY=0.080885
PRIMER_RIGHT_3_PENALTY=0.007752
PRIMER_LEFT_3_SEQUENCE=CACCAGAGTGATGTGTGGAGTTA
PRIMER_RIGHT_3_SEQUENCE=TCCCAGTAATAGAGGTTGTCGAA
PRIMER_LEFT_3=2850,23
PRIMER_RIGHT_3=3820,23
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=971
PRIMER_PAIR_PENALTY_4=0.1905
PRIMER_LEFT_4_PENALTY=0.080885
PRIMER_RIGHT_4_PENALTY=0.109631
PRIMER_LEFT_4_SEQUENCE=CACCAGAGTGATGTGTGGAGTTA
PRIMER_RIGHT_4_SEQUENCE=AGTCAATCATCCAACATTTGACC
PRIMER_LEFT_4=2850,23
PRIMER_RIGHT_4=3036,23
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=187
PRIMER_PAIR_PENALTY_5=0.2264
PRIMER_LEFT_5_PENALTY=0.218610
PRIMER_RIGHT_5_PENALTY=0.007752
PRIMER_LEFT_5_SEQUENCE=AATATGTGAACCAGCCAGATGTT
PRIMER_RIGHT_5_SEQUENCE=TCCCAGTAATAGAGGTTGTCGAA
PRIMER_LEFT_5=3562,23
PRIMER_RIGHT_5=3820,23
PRIMER_LEFT_5_TM=59.781
PRIMER_RIGHT_5_TM=60.008
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=8.7000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=259
PRIMER_PAIR_PENALTY_6=0.2495
PRIMER_LEFT_6_PENALTY=0.218610
PRIMER_RIGHT_6_PENALTY=0.030877
PRIMER_LEFT_6_SEQUENCE=AATATGTGAACCAGCCAGATGTT
PRIMER_RIGHT_6_SEQUENCE=CTAAGGCTTTCAGTACCCAGGAT
PRIMER_LEFT_6=3562,23
PRIMER_RIGHT_6=4236,23
PRIMER_LEFT_6_TM=59.781
PRIMER_RIGHT_6_TM=60.031
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=7.8000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=675
PRIMER_PAIR_PENALTY_7=0.2709
PRIMER_LEFT_7_PENALTY=0.263142
PRIMER_RIGHT_7_PENALTY=0.007752
PRIMER_LEFT_7_SEQUENCE=GAATATGTGAACCAGCCAGATGT
PRIMER_RIGHT_7_SEQUENCE=TCCCAGTAATAGAGGTTGTCGAA
PRIMER_LEFT_7=3561,23
PRIMER_RIGHT_7=3820,23
PRIMER_LEFT_7_TM=60.263
PRIMER_RIGHT_7_TM=60.008
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=8.7000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=260
PRIMER_PAIR_PENALTY_8=0.2765
PRIMER_LEFT_8_PENALTY=0.218610
PRIMER_RIGHT_8_PENALTY=0.057845
PRIMER_LEFT_8_SEQUENCE=AATATGTGAACCAGCCAGATGTT
PRIMER_RIGHT_8_SEQUENCE=ATACTGACACCATTGCTGTTCCT
PRIMER_LEFT_8=3562,23
PRIMER_RIGHT_8=4362,23
PRIMER_LEFT_8_TM=59.781
PRIMER_RIGHT_8_TM=59.942
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.6000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=801
PRIMER_PAIR_PENALTY_9=0.2778
PRIMER_LEFT_9_PENALTY=0.218610
PRIMER_RIGHT_9_PENALTY=0.059210
PRIMER_LEFT_9_SEQUENCE=AATATGTGAACCAGCCAGATGTT
PRIMER_RIGHT_9_SEQUENCE=TCGCTTTTGTTCTTAGACACTCC
PRIMER_LEFT_9=3562,23
PRIMER_RIGHT_9=4292,23
PRIMER_LEFT_9_TM=59.781
PRIMER_RIGHT_9_TM=59.941
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.6000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=731
PRIMER_PAIR_PENALTY_10=0.2910
PRIMER_LEFT_10_PENALTY=0.210152
PRIMER_RIGHT_10_PENALTY=0.080885
PRIMER_LEFT_10_SEQUENCE=ACGAGACAGAGTACCATGCAGAT
PRIMER_RIGHT_10_SEQUENCE=TAACTCCACACATCACTCTGGTG
PRIMER_LEFT_10=2767,23
PRIMER_RIGHT_10=2872,23
PRIMER_LEFT_10_TM=60.210
PRIMER_RIGHT_10_TM=60.081
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=106
PRIMER_PAIR_PENALTY_11=0.2940
PRIMER_LEFT_11_PENALTY=0.263142
PRIMER_RIGHT_11_PENALTY=0.030877
PRIMER_LEFT_11_SEQUENCE=GAATATGTGAACCAGCCAGATGT
PRIMER_RIGHT_11_SEQUENCE=CTAAGGCTTTCAGTACCCAGGAT
PRIMER_LEFT_11=3561,23
PRIMER_RIGHT_11=4236,23
PRIMER_LEFT_11_TM=60.263
PRIMER_RIGHT_11_TM=60.031
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.3000
PRIMER_RIGHT_11_END_STABILITY=7.8000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=676
PRIMER_PAIR_PENALTY_12=0.2995
PRIMER_LEFT_12_PENALTY=0.080885
PRIMER_RIGHT_12_PENALTY=0.218610
PRIMER_LEFT_12_SEQUENCE=CACCAGAGTGATGTGTGGAGTTA
PRIMER_RIGHT_12_SEQUENCE=AACATCTGGCTGGTTCACATATT
PRIMER_LEFT_12=2850,23
PRIMER_RIGHT_12=3584,23
PRIMER_LEFT_12_TM=60.081
PRIMER_RIGHT_12_TM=59.781
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=5.8000
PRIMER_PAIR_12_COMPL_ANY=7.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=735
PRIMER_PAIR_PENALTY_13=0.3198
PRIMER_LEFT_13_PENALTY=0.210152
PRIMER_RIGHT_13_PENALTY=0.109631
PRIMER_LEFT_13_SEQUENCE=ACGAGACAGAGTACCATGCAGAT
PRIMER_RIGHT_13_SEQUENCE=AGTCAATCATCCAACATTTGACC
PRIMER_LEFT_13=2767,23
PRIMER_RIGHT_13=3036,23
PRIMER_LEFT_13_TM=60.210
PRIMER_RIGHT_13_TM=60.110
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=270
PRIMER_PAIR_PENALTY_14=0.3219
PRIMER_LEFT_14_PENALTY=0.080885
PRIMER_RIGHT_14_PENALTY=0.241056
PRIMER_LEFT_14_SEQUENCE=CACCAGAGTGATGTGTGGAGTTA
PRIMER_RIGHT_14_SEQUENCE=TCCTGGTCCCAGTAATAGAGGTT
PRIMER_LEFT_14=2850,23
PRIMER_RIGHT_14=3826,23
PRIMER_LEFT_14_TM=60.081
PRIMER_RIGHT_14_TM=60.241
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=977
PRIMER_PAIR_PENALTY_15=0.3224
PRIMER_LEFT_15_PENALTY=0.263142
PRIMER_RIGHT_15_PENALTY=0.059210
PRIMER_LEFT_15_SEQUENCE=GAATATGTGAACCAGCCAGATGT
PRIMER_RIGHT_15_SEQUENCE=TCGCTTTTGTTCTTAGACACTCC
PRIMER_LEFT_15=3561,23
PRIMER_RIGHT_15=4292,23
PRIMER_LEFT_15_TM=60.263
PRIMER_RIGHT_15_TM=59.941
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.3000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=732
PRIMER_PAIR_PENALTY_16=0.3537
PRIMER_LEFT_16_PENALTY=0.301227
PRIMER_RIGHT_16_PENALTY=0.052460
PRIMER_LEFT_16_SEQUENCE=CAAAGTGTTGAGGGAAAACACAT
PRIMER_RIGHT_16_SEQUENCE=GGTACTCTGTCTCGTCAATGTCC
PRIMER_LEFT_16=2405,23
PRIMER_RIGHT_16=2781,23
PRIMER_LEFT_16_TM=60.301
PRIMER_RIGHT_16_TM=60.052
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=377
PRIMER_PAIR_PENALTY_17=0.3779
PRIMER_LEFT_17_PENALTY=0.370176
PRIMER_RIGHT_17_PENALTY=0.007752
PRIMER_LEFT_17_SEQUENCE=CTCTACAGCGGTACAGTGAGGAC
PRIMER_RIGHT_17_SEQUENCE=TCCCAGTAATAGAGGTTGTCGAA
PRIMER_LEFT_17=3472,23
PRIMER_RIGHT_17=3820,23
PRIMER_LEFT_17_TM=60.370
PRIMER_RIGHT_17_TM=60.008
PRIMER_LEFT_17_GC_PERCENT=56.522
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=8.7000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=349
PRIMER_PAIR_PENALTY_18=0.3821
PRIMER_LEFT_18_PENALTY=0.301227
PRIMER_RIGHT_18_PENALTY=0.080885
PRIMER_LEFT_18_SEQUENCE=CAAAGTGTTGAGGGAAAACACAT
PRIMER_RIGHT_18_SEQUENCE=TAACTCCACACATCACTCTGGTG
PRIMER_LEFT_18=2405,23
PRIMER_RIGHT_18=2872,23
PRIMER_LEFT_18_TM=60.301
PRIMER_RIGHT_18_TM=60.081
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=468
PRIMER_PAIR_PENALTY_19=0.4011
PRIMER_LEFT_19_PENALTY=0.370176
PRIMER_RIGHT_19_PENALTY=0.030877
PRIMER_LEFT_19_SEQUENCE=CTCTACAGCGGTACAGTGAGGAC
PRIMER_RIGHT_19_SEQUENCE=CTAAGGCTTTCAGTACCCAGGAT
PRIMER_LEFT_19=3472,23
PRIMER_RIGHT_19=4236,23
PRIMER_LEFT_19_TM=60.370
PRIMER_RIGHT_19_TM=60.031
PRIMER_LEFT_19_GC_PERCENT=56.522
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=8.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=7.6000
PRIMER_RIGHT_19_END_STABILITY=7.8000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=765
=