PRIMER_SEQUENCE_ID=NM_004560
SEQUENCE=AGCCAGCCCTTGCCGTGGCCGGAGCCGAGCGGCGCATCCGGGCCGGAGAAGAGGACGACGACGAGGTCCTCGAAGTGGACCCGTTTGCGAAGCGCCAGGGAGAAGGAGGAGCGGACGCATCGTAGAAAGGGGTGGTGGCGCCCGACCCCGCGCCCCGGCCCGAAGCTCTGAGGGCTTCCCGGCCCCCACTGCCTGCGGCATGGCCCGGGGCTCGGCGCTCCCGCGGCGGCCGCTGCTGTGCATCCCGGCCGTCTGGGCGGCCGCCGCGCTTCTGCTCTCAGTGTCCCGGACTTCAGGTGAAGTGGAGGTTCTGGATCCGAACGACCCTTTAGGACCCCTTGATGGGCAGGACGGCCCGATTCCAACTCTGAAAGGTTACTTTCTGAATTTTCTGGAGCCAGTAAACAATATCACCATTGTCCAAGGCCAGACGGCAATTCTGCACTGCAAGGTGGCAGGAAACCCACCCCCTAACGTGCGGTGGCTAAAGAATGATGCCCCGGTGGTGCAGGAGCCGCGGCGGATCATCATCCGGAAGACAGAATATGGTTCACGACTGCGAATCCAGGACCTGGACACGACAGACACTGGCTACTACCAGTGCGTGGCCACCAACGGGATGAAGACCATTACCGCCACTGGCGTCCTGTTTGTGCGGCTGGGTCCAACGCACAGCCCAAATCATAACTTTCAGGATGATTACCACGAGGATGGGTTCTGCCAGCCTTACCGGGGAATTGCCTGTGCACGCTTCATTGGCAACCGGACCATTTATGTGGACTCGCTTCAGATGCAGGGGGAGATTGAAAACCGAATCACAGCGGCCTTCACCATGATCGGCACGTCTACGCACCTGTCGGACCAGTGCTCACAGTTCGCCATCCCATCCTTCTGCCACTTCGTGTTTCCTCTGTGCGACGCGCGCTCCCGGACACCCAAGCCGCGTGAGCTGTGCCGCGACGAGTGCGAGGTGCTGGAGAGCGACCTGTGCCGCCAGGAGTACACCATCGCCCGCTCCAACCCGCTCATCCTCATGCGGCTTCAGCTGCCCAAGTGTGAGGCGCTGCCCATGCCTGAGAGCCCCGACGCTGCCAACTGCATGCGCATTGGCATCCCAGCCGAGAGGCTGGGCCGCTACCATCAGTGCTATAACGGCTCAGGCATGGATTACAGAGGAACGGCAAGCACCACCAAGTCAGGCCACCAGTGCCAGCCGTGGGCCCTGCAGCACCCCCACAGCCACCACCTGTCCAGCACAGACTTCCCTGAGCTTGGAGGGGGGCACGCCTACTGCCGGAACCCCGGAGGCCAGATGGAGGGCCCCTGGTGCTTTACGCAGAATAAAAACGTACGCATGGAACTGTGTGACGTACCCTCGTGTAGTCCCCGAGACAGCAGCAAGATGGGGATTCTGTACATCTTGGTCCCCAGCATCGCAATTCCACTGGTCATCGCTTGCCTTTTCTTCTTGGTTTGCATGTGCCGGAATAAGCAGAAGGCATCTGCGTCCACACCGCAGCGGCGACAGCTGATGGCCTCGCCCAGCCAAGACATGGAAATGCCCCTCATTAACCAGCACAAACAGGCCAAACTCAAAGAGATCAGCCTGTCTGCGGTGAGGTTCATGGAGGAGCTGGGAGAGGACCGGTTTGGGAAAGTCTACAAAGGTCACCTGTTCGGCCCTGCCCCGGGGGAGCAGACCCAGGCTGTGGCCATCAAAACGCTGAAGGACAAAGCGGAGGGGCCCCTGCGGGAGGAGTTCCGGCATGAGGCTATGCTGCGAGCACGGCTGCAACACCCCAACGTCGTCTGCCTGCTGGGCGTGGTGACCAAGGACCAGCCCCTGAGCATGATCTTCAGCTACTGTTCGCACGGCGACCTCCACGAATTCCTGGTCATGCGCTCGCCGCACTCGGACGTGGGCAGCACCGATGATGACCGCACGGTGAAGTCCGCCCTGGAGCCCCCCGACTTCGTGCACCTTGTGGCACAGATCGCGGCGGGGATGGAGTACCTATCCAGCCACCACGTGGTTCACAAGGACCTGGCCACCCGCAATGTGCTAGTGTACGACAAGCTGAACGTGAAGATCTCAGACTTGGGCCTCTTCCGAGAGGTGTATGCCGCCGATTACTACAAGCTGCTGGGGAACTCGCTGCTGCCTATCCGCTGGATGGCCCCAGAGGCCATCATGTACGGCAAGTTCTCCATCGACTCAGACATCTGGTCCTACGGTGTGGTCCTGTGGGAGGTCTTCAGCTACGGCCTGCAGCCCTACTGCGGGTACTCCAACCAGGATGTGGTGGAGATGATCCGGAACCGGCAGGTGCTGCCTTGCCCCGATGACTGTCCCGCCTGGGTGTATGCCCTCATGATCGAGTGCTGGAACGAGTTCCCCAGCCGGCGGCCCCGCTTCAAGGACATCCACAGCCGGCTCCGAGCCTGGGGCAACCTTTCCAACTACAACAGCTCGGCGCAGACCTCGGGGGCCAGCAACACCACGCAGACCAGCTCCCTGAGCACCAGCCCAGTGAGCAATGTGAGCAACGCCCGCTACGTGGGGCCCAAGCAGAAGGCCCCGCCCTTCCCACAGCCCCAGTTCATCCCCATGAAGGGCCAGATCAGACCCATGGTGCCCCCGCCGCAGCTCTACGTCCCCGTCAACGGCTACCAGCCGGTGCCGGCCTATGGGGCCTACCTGCCCAACTTCTACCCGGTGCAGATCCCAATGCAGATGGCCCCGCAGCAGGTGCCTCCTCAGATGGTCCCCAAGCCCAGCTCACACCACAGTGGCAGTGGCTCCACCAGCACAGGCTACGTCACCACGGCCCCCTCCAACACATCCATGGCAGACAGGGCAGCCCTGCTCTCAGAGGGCGCTGATGACACACAGAACGCCCCAGAAGATGGGGCCCAGAGCACCGTGCAGGAAGCAGAGGAGGAGGAGGAAGGCTCTGTCCCAGAGACTGAGCTGCTGGGGGACTGTGACACTCTGCAGGTGGACGAGGCCCAAGTCCAGCTGGAAGCTTGAGTGGCACCAGGGCCCGGGGTTCGGGGATAGAAGCCCCGCCGAGACCCCACAGGGACCTCAGTCACCTTTGAGAAGACACCATACTCAGCAATCACAAGAGCCCGCCGGCCAGTGGGCTTGTTTGCAGACTGGGTGAGGTGGAGCCCTGCTCCTCTCTGTCCTCTGACACAGAGAGCTGCCCTGCCTAGGAGCACCCAAGCCAGGCAGGGGGTCTGGCAGCACGGCGTCCTGGGGAGCAGGACACATGGTCATCCCCAGGGCTGTATACATTGATTCTGGTGGTAGACTGGTAGTGAGCAGCAAATGCCTTTCAAGAAAATAGGTGGCAGCTTCACTCCATGTCATATATGGAGTGAATATTTCAAAACGTTGGGAATAAGGGCCTGCAAAAGGCAGCGAGGAGGCACCTCGGGTCTTGAGGTTCCTGACAACCGATCTGGTCTGTTGGTTTGAGGATGAAGGGGCTCCATTTCTGCTGCCTCCCTGCTGAGAATATTCTCCCTTTAGCAGCCAAAGATTCGCTGGAACGGAGGCTGCCCTCTGCTGCCTGTTGGGGTCGGAAGACAAGGGGCTTCTGAAATGGGAGTTCCTGAGATACAACAAAATGTGTGCCTTCAAAGAAACTGACAGCTTTGTATTTGGTGAAATGGTTTTAATTATACTCCATGTGTATTTTGCCCACTTTTTTTGGGAATTCAAGGGAAAGTGTTTCTTGGGTTTGGAATGTTCAGAGGAAGCAGTATTGTACAGAACACGGTATTGTTATTTTTGTTAAGAATCATGTACAGAGCTTAAATGTAATTTATATGTTTTTAATATGCCATTTTCATTGAAGTATTTTGGTCTTAAGATGACTTTAGTAATTTAACTGTTTATGTTACCCACGTTGGGATCCAGTTGGTCTTGGTTTGCTTCTCTCTGTACCACGTGCACATGAGGTCCATTCATTTTACAGCCCCTGTTACACACAGACCCACAGGCAGCCGTCTGTGCCCCGCACACATTGTTGGTCCTATTTGTAAATCCCACACCCGGTGTATCCAATAAAGTGAAACAAAGC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47413, GC content failed 2117, low tm 11224, high tm 25609, high any compl 14, high end compl 76, high repeat similarity 90, long poly-x seq 64, ok 8219
PRIMER_RIGHT_EXPLAIN=considered 47422, GC content failed 1939, low tm 11468, high tm 25469, high any compl 1, high end compl 9, high repeat similarity 17, long poly-x seq 64, ok 8455
PRIMER_PAIR_EXPLAIN=considered 4093, unacceptable product size 3997, high end compl 10, ok 86
PRIMER_PAIR_PENALTY=0.2049
PRIMER_LEFT_PENALTY=0.071885
PRIMER_RIGHT_PENALTY=0.132987
PRIMER_LEFT_SEQUENCE=TGAGAAGACACCATACTCAGCAA
PRIMER_RIGHT_SEQUENCE=ACGTGGTACAGAGAGAAGCAAAC
PRIMER_LEFT=3099,23
PRIMER_RIGHT=3959,23
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=861
PRIMER_PAIR_PENALTY_1=0.2049
PRIMER_LEFT_1_PENALTY=0.071885
PRIMER_RIGHT_1_PENALTY=0.132987
PRIMER_LEFT_1_SEQUENCE=TTGAGAAGACACCATACTCAGCA
PRIMER_RIGHT_1_SEQUENCE=ACGTGGTACAGAGAGAAGCAAAC
PRIMER_LEFT_1=3098,23
PRIMER_RIGHT_1=3959,23
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=862
PRIMER_PAIR_PENALTY_2=0.2246
PRIMER_LEFT_2_PENALTY=0.091636
PRIMER_RIGHT_2_PENALTY=0.132987
PRIMER_LEFT_2_SEQUENCE=GACCTCAGTCACCTTTGAGAAGA
PRIMER_RIGHT_2_SEQUENCE=ACGTGGTACAGAGAGAAGCAAAC
PRIMER_LEFT_2=3084,23
PRIMER_RIGHT_2=3959,23
PRIMER_LEFT_2_TM=59.908
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=876
PRIMER_PAIR_PENALTY_3=0.2534
PRIMER_LEFT_3_PENALTY=0.189204
PRIMER_RIGHT_3_PENALTY=0.064245
PRIMER_LEFT_3_SEQUENCE=GTGCTTTACGCAGAATAAAAACG
PRIMER_RIGHT_3_SEQUENCE=ACAGGCTGATCTCTTTGAGTTTG
PRIMER_LEFT_3=1326,23
PRIMER_RIGHT_3=1609,23
PRIMER_LEFT_3_TM=60.189
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.3037
PRIMER_LEFT_4_PENALTY=0.189204
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=GTGCTTTACGCAGAATAAAAACG
PRIMER_RIGHT_4_SEQUENCE=GCTTGTCGTACACTAGCACATTG
PRIMER_LEFT_4=1326,23
PRIMER_RIGHT_4=2077,23
PRIMER_LEFT_4_TM=60.189
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=752
PRIMER_PAIR_PENALTY_5=0.3089
PRIMER_LEFT_5_PENALTY=0.071885
PRIMER_RIGHT_5_PENALTY=0.236972
PRIMER_LEFT_5_SEQUENCE=TGAGAAGACACCATACTCAGCAA
PRIMER_RIGHT_5_SEQUENCE=GCTCACTACCAGTCTACCACCAG
PRIMER_LEFT_5=3099,23
PRIMER_RIGHT_5=3328,23
PRIMER_LEFT_5_TM=59.928
PRIMER_RIGHT_5_TM=60.237
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=56.522
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MER39 a consensus
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=230
PRIMER_PAIR_PENALTY_6=0.3089
PRIMER_LEFT_6_PENALTY=0.071885
PRIMER_RIGHT_6_PENALTY=0.236972
PRIMER_LEFT_6_SEQUENCE=TTGAGAAGACACCATACTCAGCA
PRIMER_RIGHT_6_SEQUENCE=GCTCACTACCAGTCTACCACCAG
PRIMER_LEFT_6=3098,23
PRIMER_RIGHT_6=3328,23
PRIMER_LEFT_6_TM=59.928
PRIMER_RIGHT_6_TM=60.237
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=56.522
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.5000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=231
PRIMER_PAIR_PENALTY_7=0.3089
PRIMER_LEFT_7_PENALTY=0.071885
PRIMER_RIGHT_7_PENALTY=0.236972
PRIMER_LEFT_7_SEQUENCE=TGAGAAGACACCATACTCAGCAA
PRIMER_RIGHT_7_SEQUENCE=CTGCTCACTACCAGTCTACCACC
PRIMER_LEFT_7=3099,23
PRIMER_RIGHT_7=3330,23
PRIMER_LEFT_7_TM=59.928
PRIMER_RIGHT_7_TM=60.237
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=56.522
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=232
PRIMER_PAIR_PENALTY_8=0.3089
PRIMER_LEFT_8_PENALTY=0.071885
PRIMER_RIGHT_8_PENALTY=0.236972
PRIMER_LEFT_8_SEQUENCE=TTGAGAAGACACCATACTCAGCA
PRIMER_RIGHT_8_SEQUENCE=CTGCTCACTACCAGTCTACCACC
PRIMER_LEFT_8=3098,23
PRIMER_RIGHT_8=3330,23
PRIMER_LEFT_8_TM=59.928
PRIMER_RIGHT_8_TM=60.237
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=56.522
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=233
PRIMER_PAIR_PENALTY_9=0.3286
PRIMER_LEFT_9_PENALTY=0.091636
PRIMER_RIGHT_9_PENALTY=0.236972
PRIMER_LEFT_9_SEQUENCE=GACCTCAGTCACCTTTGAGAAGA
PRIMER_RIGHT_9_SEQUENCE=CTGCTCACTACCAGTCTACCACC
PRIMER_LEFT_9=3084,23
PRIMER_RIGHT_9=3330,23
PRIMER_LEFT_9_TM=59.908
PRIMER_RIGHT_9_TM=60.237
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=56.522
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=247
PRIMER_PAIR_PENALTY_10=0.3286
PRIMER_LEFT_10_PENALTY=0.091636
PRIMER_RIGHT_10_PENALTY=0.236972
PRIMER_LEFT_10_SEQUENCE=GACCTCAGTCACCTTTGAGAAGA
PRIMER_RIGHT_10_SEQUENCE=GCTCACTACCAGTCTACCACCAG
PRIMER_LEFT_10=3084,23
PRIMER_RIGHT_10=3328,23
PRIMER_LEFT_10_TM=59.908
PRIMER_RIGHT_10_TM=60.237
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=56.522
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=245
PRIMER_PAIR_PENALTY_11=0.3433
PRIMER_LEFT_11_PENALTY=0.279044
PRIMER_RIGHT_11_PENALTY=0.064245
PRIMER_LEFT_11_SEQUENCE=CTACCATCAGTGCTATAACGGCT
PRIMER_RIGHT_11_SEQUENCE=ACAGGCTGATCTCTTTGAGTTTG
PRIMER_LEFT_11=1134,23
PRIMER_RIGHT_11=1609,23
PRIMER_LEFT_11_TM=59.721
PRIMER_RIGHT_11_TM=59.936
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=11.4000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=476
PRIMER_PAIR_PENALTY_12=0.3542
PRIMER_LEFT_12_PENALTY=0.071885
PRIMER_RIGHT_12_PENALTY=0.282361
PRIMER_LEFT_12_SEQUENCE=TGAGAAGACACCATACTCAGCAA
PRIMER_RIGHT_12_SEQUENCE=GTGTAACAGGGGCTGTAAAATGA
PRIMER_LEFT_12=3099,23
PRIMER_RIGHT_12=3997,23
PRIMER_LEFT_12_TM=59.928
PRIMER_RIGHT_12_TM=60.282
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=899
PRIMER_PAIR_PENALTY_13=0.3542
PRIMER_LEFT_13_PENALTY=0.071885
PRIMER_RIGHT_13_PENALTY=0.282361
PRIMER_LEFT_13_SEQUENCE=TTGAGAAGACACCATACTCAGCA
PRIMER_RIGHT_13_SEQUENCE=GTGTAACAGGGGCTGTAAAATGA
PRIMER_LEFT_13=3098,23
PRIMER_RIGHT_13=3997,23
PRIMER_LEFT_13_TM=59.928
PRIMER_RIGHT_13_TM=60.282
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=900
PRIMER_PAIR_PENALTY_14=0.3700
PRIMER_LEFT_14_PENALTY=0.236972
PRIMER_RIGHT_14_PENALTY=0.132987
PRIMER_LEFT_14_SEQUENCE=GGTGGTAGACTGGTAGTGAGCAG
PRIMER_RIGHT_14_SEQUENCE=ACGTGGTACAGAGAGAAGCAAAC
PRIMER_LEFT_14=3308,23
PRIMER_RIGHT_14=3959,23
PRIMER_LEFT_14_TM=60.237
PRIMER_RIGHT_14_TM=59.867
PRIMER_LEFT_14_GC_PERCENT=56.522
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=652
PRIMER_PAIR_PENALTY_15=0.3700
PRIMER_LEFT_15_PENALTY=0.236972
PRIMER_RIGHT_15_PENALTY=0.132987
PRIMER_LEFT_15_SEQUENCE=CTGGTGGTAGACTGGTAGTGAGC
PRIMER_RIGHT_15_SEQUENCE=ACGTGGTACAGAGAGAAGCAAAC
PRIMER_LEFT_15=3306,23
PRIMER_RIGHT_15=3959,23
PRIMER_LEFT_15_TM=60.237
PRIMER_RIGHT_15_TM=59.867
PRIMER_LEFT_15_GC_PERCENT=56.522
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=654
PRIMER_PAIR_PENALTY_16=0.3740
PRIMER_LEFT_16_PENALTY=0.091636
PRIMER_RIGHT_16_PENALTY=0.282361
PRIMER_LEFT_16_SEQUENCE=GACCTCAGTCACCTTTGAGAAGA
PRIMER_RIGHT_16_SEQUENCE=GTGTAACAGGGGCTGTAAAATGA
PRIMER_LEFT_16=3084,23
PRIMER_RIGHT_16=3997,23
PRIMER_LEFT_16_TM=59.908
PRIMER_RIGHT_16_TM=60.282
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=914
PRIMER_PAIR_PENALTY_17=0.3769
PRIMER_LEFT_17_PENALTY=0.187663
PRIMER_RIGHT_17_PENALTY=0.189204
PRIMER_LEFT_17_SEQUENCE=ATCATCATCCGGAAGACAGAATA
PRIMER_RIGHT_17_SEQUENCE=CGTTTTTATTCTGCGTAAAGCAC
PRIMER_LEFT_17=523,23
PRIMER_RIGHT_17=1348,23
PRIMER_LEFT_17_TM=59.812
PRIMER_RIGHT_17_TM=60.189
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=5.9000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=826
PRIMER_PAIR_PENALTY_18=0.3850
PRIMER_LEFT_18_PENALTY=0.189204
PRIMER_RIGHT_18_PENALTY=0.195841
PRIMER_LEFT_18_SEQUENCE=GTGCTTTACGCAGAATAAAAACG
PRIMER_RIGHT_18_SEQUENCE=ACCTTTGTAGACTTTCCCAAACC
PRIMER_LEFT_18=1326,23
PRIMER_RIGHT_18=1668,23
PRIMER_LEFT_18_TM=60.189
PRIMER_RIGHT_18_TM=59.804
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.7000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=343
PRIMER_PAIR_PENALTY_19=0.3935
PRIMER_LEFT_19_PENALTY=0.279044
PRIMER_RIGHT_19_PENALTY=0.114470
PRIMER_LEFT_19_SEQUENCE=CTACCATCAGTGCTATAACGGCT
PRIMER_RIGHT_19_SEQUENCE=GCTTGTCGTACACTAGCACATTG
PRIMER_LEFT_19=1134,23
PRIMER_RIGHT_19=2077,23
PRIMER_LEFT_19_TM=59.721
PRIMER_RIGHT_19_TM=59.886
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=11.4000
PRIMER_RIGHT_19_END_STABILITY=7.2000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=944
=