PRIMER_SEQUENCE_ID=NM_004972
SEQUENCE=CTGCAGGAAGGAGAGAGGAAGAGGAGCAGAAGGGGGCAGCAGCGGACGCCGCTAACGGCCTCCCTCGGCGCTGACAGGCTGGGCCGGCGCCCGGCTCGCTTGGGTGTTCGCGTCGCCACTTCGGCTTCTCGGCCGGTCGGGCCCCTCGGCCCGGGCTTGCGGCGCGCGTCGGGGCTGAGGGCTGCTGCGGCGCAGGGAGAGGCCTGGTCCTCGCTGCCGAGGGATGTGAGTGGGAGCTGAGCCCACACTGGAGGGCCCCCGAGGGCCCAGCCTGGAGGTCGTTCAGAGCCGTGCCCGCCCCGGGGCTTCGCAGACCTTGACCCGCCGGGTAGGAGCCGCCCCTGCGGGCTCGAGGGCGCGCTCTGGTCGCCCGATCTGTGTAGCCGGTTTCAGAAGCAGGCAACAGGAACAAGATGTGAACTGTTTCTCTTCTGCAGAAAAAGAGGCTCTTCCTCCTCCTCCCGCGACGGCAAATGTTCTGAAAAAGACTCTGCATGGGAATGGCCTGCCTTACGATGACAGAAATGGAGGGAACATCCACCTCTTCTATATATCAGAATGGTGATATTTCTGGAAATGCCAATTCTATGAAGCAAATAGATCCAGTTCTTCAGGTGTATCTTTACCATTCCCTTGGGAAATCTGAGGCAGATTATCTGACCTTTCCATCTGGGGAGTATGTTGCAGAAGAAATCTGTATTGCTGCTTCTAAAGCTTGTGGTATCACACCTGTGTATCATAATATGTTTGCTTTAATGAGTGAAACAGAAAGGATCTGGTATCCACCCAACCATGTCTTCCATATAGATGAGTCAACCAGGCATAATGTACTCTACAGAATAAGATTTTACTTTCCTCGTTGGTATTGCAGTGGCAGCAACAGAGCCTATCGGCATGGAATATCTCGAGGTGCTGAAGCTCCTCTTCTTGATGACTTTGTCATGTCTTACCTCTTTGCTCAGTGGCGGCATGATTTTGTGCACGGATGGATAAAAGTACCTGTGACTCATGAAACACAGGAAGAATGTCTTGGGATGGCAGTGTTAGATATGATGAGAATAGCCAAAGAAAACGATCAAACCCCACTGGCCATCTATAACTCTATCAGCTACAAGACATTCTTACCAAAATGTATTCGAGCAAAGATCCAAGACTATCATATTTTGACAAGGAAGCGAATAAGGTACAGATTTCGCAGATTTATTCAGCAATTCAGCCAATGCAAAGCCACTGCCAGAAACTTGAAACTTAAGTATCTTATAAATCTGGAAACTCTGCAGTCTGCCTTCTACACAGAGAAATTTGAAGTAAAAGAACCTGGAAGTGGTCCTTCAGGTGAGGAGATTTTTGCAACCATTATAATAACTGGAAACGGTGGAATTCAGTGGTCAAGAGGGAAACATAAAGAAAGTGAGACACTGACAGAACAGGATTTACAGTTATATTGCGATTTTCCTAATATTATTGATGTCAGTATTAAGCAAGCAAACCAAGAGGGTTCAAATGAAAGCCGAGTTGTAACTATCCATAAGCAAGATGGTAAAAATCTGGAAATTGAACTTAGCTCATTAAGGGAAGCTTTGTCTTTCGTGTCATTAATTGATGGATATTATAGATTAACTGCAGATGCACATCATTACCTCTGTAAAGAAGTAGCACCTCCAGCCGTGCTTGAAAATATACAAAGCAACTGTCATGGCCCAATTTCGATGGATTTTGCCATTAGTAAACTGAAGAAAGCAGGTAATCAGACTGGACTGTATGTACTTCGATGCAGTCCTAAGGACTTTAATAAATATTTTTTGACTTTTGCTGTCGAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTACAAAAAATGAGAATGAAGAGTACAACCTCAGTGGGACAAAGAAGAACTTCAGCAGTCTTAAAGATCTTTTGAATTGTTACCAGATGGAAACTGTTCGCTCAGACAATATAATTTTCCAGTTTACTAAATGCTGTCCCCCAAAGCCAAAAGATAAATCAAACCTTCTAGTCTTCAGAACGAATGGTGTTTCTGATGTACCAACCTCACCAACATTACAGAGGCCTACTCATATGAACCAAATGGTGTTTCACAAAATCAGAAATGAAGATTTGATATTTAATGAAAGCCTTGGCCAAGGCACTTTTACAAAGATTTTTAAAGGCGTACGAAGAGAAGTAGGAGACTACGGTCAACTGCATGAAACAGAAGTTCTTTTAAAAGTTCTGGATAAAGCACACAGAAACTATTCAGAGTCTTTCTTTGAAGCAGCAAGTATGATGAGCAAGCTTTCTCACAAGCATTTGGTTTTAAATTATGGAGTATGTGTCTGTGGAGACGAGAATATTCTGGTTCAGGAGTTTGTAAAATTTGGATCACTAGATACATATCTGAAAAAGAATAAAAATTGTATAAATATATTATGGAAACTTGAAGTTGCTAAACAGTTGGCATGGGCCATGCATTTTCTAGAAGAAAACACCCTTATTCATGGGAATGTATGTGCCAAAAATATTCTGCTTATCAGAGAAGAAGACAGGAAGACAGGAAATCCTCCTTTCATCAAACTTAGTGATCCTGGCATTAGTATTACAGTTTTGCCAAAGGACATTCTTCAGGAGAGAATACCATGGGTACCACCTGAATGCATTGAAAATCCTAAAAATTTAAATTTGGCAACAGACAAATGGAGTTTTGGTACCACTTTGTGGGAAATCTGCAGTGGAGGAGATAAACCTCTAAGTGCTCTGGATTCTCAAAGAAAGCTACAATTTTATGAAGATAGGCATCAGCTTCCTGCACCAAAGTGGGCAGAATTAGCAAACCTTATAAATAATTGTATGGATTATGAACCAGATTTCAGGCCTTCTTTCAGAGCCATCATACGAGATCTTAACAGTTTGTTTACTCCAGATTATGAACTATTAACAGAAAATGACATGTTACCAAATATGAGGATAGGTGCCCTAGGGTTTTCTGGTGCCTTTGAAGACCGGGATCCTACACAGTTTGAAGAGAGACATTTGAAATTTCTACAGCAACTTGGCAAGGGTAATTTTGGGAGTGTGGAGATGTGCCGGTATGACCCTCTACAGGACAACACTGGGGAGGTGGTCGCTGTAAAAAAGCTTCAGCATAGTACTGAAGAGCACCTAAGAGACTTTGAAAGGGAAATTGAAATCCTGAAATCCCTACAGCATGACAACATTGTAAAGTACAAGGGAGTGTGCTACAGTGCTGGTCGGCGTAATCTAAAATTAATTATGGAATATTTACCATATGGAAGTTTACGAGACTATCTTCAAAAACATAAAGAACGGATAGATCACATAAAACTTCTGCAGTACACATCTCAGATATGCAAGGGTATGGAGTATCTTGGTACAAAAAGGTATATCCACAGGGATCTGGCAACGAGAAATATATTGGTGGAGAACGAGAACAGAGTTAAAATTGGAGATTTTGGGTTAACCAAAGTCTTGCCACAAGACAAAGAATACTATAAAGTAAAAGAACCTGGTGAAAGTCCCATATTCTGGTATGCTCCAGAATCACTGACAGAGAGCAAGTTTTCTGTGGCCTCAGATGTTTGGAGCTTTGGAGTGGTTCTGTATGAACTTTTCACATACATTGAGAAGAGTAAAAGTCCACCAGCGGAATTTATGCGTATGATTGGCAATGACAAACAAGGACAGATGATCGTGTTCCATTTGATAGAACTTTTGAAGAATAATGGAAGATTACCAAGACCAGATGGATGCCCAGATGAGATCTATATGATCATGACAGAATGCTGGAACAATAATGTAAATCAACGCCCCTCCTTTAGGGATCTAGCTCTTCGAGTGGATCAAATAAGGGATAACATGGCTGGATGAAAGAAATGACCTTCATTCTGAGACCAAAGTAGATTTACAGAACAAAGTTTTATATTTCACATTGCTGTGGACTATTATTACATATATCATTATTATATAAATCATGATGCTAGCCAGCAAAGATGTGAAAATATCTGCTCAAAACTTTCAAAGTTTAGTAAGTTTTTCTTCATGAGGCCACCAGTAAAAGACATTAATGAGAATTCCTTAGCAAGGATTTTGTAAGAAGTTTCTTAAACATTGTCTGTTAACATCACTCTTGTCTGGCAAAAGAAAAAAAATAGACTTTTTCAACTCAGCTTTTTGAGACCTGAAAAAATTATTATGTAAATTTTGCAATGTTAAAGATGCACAGAATATGTATGTATAGTTTTTACCACAGTGGATGTATAATACCTTGGCATCTTGTGTGATGTTTTACACACATGAGGGCTGGTGTTCATTAATACTGTTTTCTAATTTTTCCATAGTTAATCTATAATTAATTACTTCACTATACAAACAAATTAAGATGTTCAGATAATTGAATAAGTACCTTTGTGTCCTTGTTCATTTATATCGCTGGCCAGCATTATAAGCAGGTGTATACTTTTAGCTTGTAGTTCCATGTACTGTAAATATTTTTCACATAAAGGGAACAAATGTCTAGTTTTATTTGTATAGGAAATTTCCCTGACCCTAAATAATACATTTTGAAATGAAACAAGCTTACAAAGATATAATCTATTTTATTATGGTTTCCCTTGTATCTATTTGTGGTGAATGTGTTTTTTAAATGGAACTATCTCCAAATTTTTCTAAGACTACTATGAACAGTTTTCTTTTAAAATTTTGAGATTAAGAATGCCAGGAATATTGTCATCCTTTGAGCTGCTGACTGCCAATAACATTCTTCGATCTCTGGGATTTATGCTCATGAACTAAATTTAAGCTTAAGCCATAAAATAGATTAGATTGTTTTTTAAAAATGGATAGCTCATTAAGAAGTGCAGCAGGTTAAGAATTTTTTCCTAAAGACTGTATATTTGAGGGGTTTCAGAATTTTGCATTGCAGTCATAGAAGAGATTTATTTCCTTTTTAGAGGGGAAATGAGGTAAATAAGTAAAAAAGTATGCTTGTTAATTTTATTCAAGAATGCCAGTAGAAAATTCATAACGTGTATCTTTAAGAAAAATGAGCATACATCTTAAATCTTTTCAATTA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 58841, GC content failed 4576, low tm 33429, high tm 8968, high any compl 9, high end compl 58, high repeat similarity 39, long poly-x seq 162, ok 11600
PRIMER_RIGHT_EXPLAIN=considered 58843, GC content failed 4529, low tm 34081, high tm 8401, high any compl 2, high end compl 16, high repeat similarity 11, long poly-x seq 163, ok 11640
PRIMER_PAIR_EXPLAIN=considered 1386, unacceptable product size 1319, high end compl 10, ok 57
PRIMER_PAIR_PENALTY=0.0205
PRIMER_LEFT_PENALTY=0.014220
PRIMER_RIGHT_PENALTY=0.006308
PRIMER_LEFT_SEQUENCE=TCAAACTTAGTGATCCTGGCATT
PRIMER_RIGHT_SEQUENCE=CTCCATACCCTTGCATATCTGAG
PRIMER_LEFT=2577,23
PRIMER_RIGHT=3388,23
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=812
PRIMER_PAIR_PENALTY_1=0.0284
PRIMER_LEFT_1_PENALTY=0.014220
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=ACCAAATGGTGTTTCACAAAATC
PRIMER_RIGHT_1_SEQUENCE=AATGCCAGGATCACTAAGTTTGA
PRIMER_LEFT_1=2091,23
PRIMER_RIGHT_1=2599,23
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=34.783
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=509
PRIMER_PAIR_PENALTY_2=0.0294
PRIMER_LEFT_2_PENALTY=0.014220
PRIMER_RIGHT_2_PENALTY=0.015214
PRIMER_LEFT_2_SEQUENCE=ACCAAATGGTGTTTCACAAAATC
PRIMER_RIGHT_2_SEQUENCE=AGGTGGTACCCATGGTATTCTCT
PRIMER_LEFT_2=2091,23
PRIMER_RIGHT_2=2656,23
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=60.015
PRIMER_LEFT_2_GC_PERCENT=34.783
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=566
PRIMER_PAIR_PENALTY_3=0.0470
PRIMER_LEFT_3_PENALTY=0.032744
PRIMER_RIGHT_3_PENALTY=0.014220
PRIMER_LEFT_3_SEQUENCE=GTCTTCAGAACGAATGGTGTTTC
PRIMER_RIGHT_3_SEQUENCE=AATGCCAGGATCACTAAGTTTGA
PRIMER_LEFT_3=2024,23
PRIMER_RIGHT_3=2599,23
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MER34 a consensus
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=576
PRIMER_PAIR_PENALTY_4=0.0480
PRIMER_LEFT_4_PENALTY=0.032744
PRIMER_RIGHT_4_PENALTY=0.015214
PRIMER_LEFT_4_SEQUENCE=GTCTTCAGAACGAATGGTGTTTC
PRIMER_RIGHT_4_SEQUENCE=AGGTGGTACCCATGGTATTCTCT
PRIMER_LEFT_4=2024,23
PRIMER_RIGHT_4=2656,23
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=633
PRIMER_PAIR_PENALTY_5=0.0497
PRIMER_LEFT_5_PENALTY=0.016975
PRIMER_RIGHT_5_PENALTY=0.032744
PRIMER_LEFT_5_SEQUENCE=ATCATATTTTGACAAGGAAGCGA
PRIMER_RIGHT_5_SEQUENCE=GGTTGGTACATCAGAAACACCAT
PRIMER_LEFT_5=1155,23
PRIMER_RIGHT_5=2059,23
PRIMER_LEFT_5_TM=59.983
PRIMER_RIGHT_5_TM=60.033
PRIMER_LEFT_5_GC_PERCENT=34.783
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=9.9000
PRIMER_RIGHT_5_END_STABILITY=7.8000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=905
PRIMER_PAIR_PENALTY_6=0.0497
PRIMER_LEFT_6_PENALTY=0.016975
PRIMER_RIGHT_6_PENALTY=0.032744
PRIMER_LEFT_6_SEQUENCE=AAATGTATTCGAGCAAAGATCCA
PRIMER_RIGHT_6_SEQUENCE=GGTTGGTACATCAGAAACACCAT
PRIMER_LEFT_6=1127,23
PRIMER_RIGHT_6=2059,23
PRIMER_LEFT_6_TM=59.983
PRIMER_RIGHT_6_TM=60.033
PRIMER_LEFT_6_GC_PERCENT=34.783
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=7.8000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=933
PRIMER_PAIR_PENALTY_7=0.0497
PRIMER_LEFT_7_PENALTY=0.016975
PRIMER_RIGHT_7_PENALTY=0.032744
PRIMER_LEFT_7_SEQUENCE=AATGTATTCGAGCAAAGATCCAA
PRIMER_RIGHT_7_SEQUENCE=GGTTGGTACATCAGAAACACCAT
PRIMER_LEFT_7=1128,23
PRIMER_RIGHT_7=2059,23
PRIMER_LEFT_7_TM=59.983
PRIMER_RIGHT_7_TM=60.033
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=7.8000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=932
PRIMER_PAIR_PENALTY_8=0.0524
PRIMER_LEFT_8_PENALTY=0.010529
PRIMER_RIGHT_8_PENALTY=0.041875
PRIMER_LEFT_8_SEQUENCE=CATGACAGAATGCTGGAACAATA
PRIMER_RIGHT_8_SEQUENCE=TTTTCAGGTCTCAAAAAGCTGAG
PRIMER_LEFT_8=3796,23
PRIMER_RIGHT_8=4209,23
PRIMER_LEFT_8_TM=60.011
PRIMER_RIGHT_8_TM=60.042
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.2000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=414
PRIMER_PAIR_PENALTY_9=0.0526
PRIMER_LEFT_9_PENALTY=0.010725
PRIMER_RIGHT_9_PENALTY=0.041875
PRIMER_LEFT_9_SEQUENCE=GCCAGCAAAGATGTGAAAATATC
PRIMER_RIGHT_9_SEQUENCE=TTTTCAGGTCTCAAAAAGCTGAG
PRIMER_LEFT_9=4005,23
PRIMER_RIGHT_9=4209,23
PRIMER_LEFT_9_TM=59.989
PRIMER_RIGHT_9_TM=60.042
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=8.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.5000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=205
PRIMER_PAIR_PENALTY_10=0.0530
PRIMER_LEFT_10_PENALTY=0.038744
PRIMER_RIGHT_10_PENALTY=0.014220
PRIMER_LEFT_10_SEQUENCE=TGTAAAGAAGTAGCACCTCCAGC
PRIMER_RIGHT_10_SEQUENCE=AATGCCAGGATCACTAAGTTTGA
PRIMER_LEFT_10=1643,23
PRIMER_RIGHT_10=2599,23
PRIMER_LEFT_10_TM=59.961
PRIMER_RIGHT_10_TM=60.014
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, reverse MER34 a consensus
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=7.3000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=957
PRIMER_PAIR_PENALTY_11=0.0551
PRIMER_LEFT_11_PENALTY=0.016975
PRIMER_RIGHT_11_PENALTY=0.038125
PRIMER_LEFT_11_SEQUENCE=ATCATATTTTGACAAGGAAGCGA
PRIMER_RIGHT_11_SEQUENCE=GCTTTGTATATTTTCAAGCACGG
PRIMER_LEFT_11=1155,23
PRIMER_RIGHT_11=1687,23
PRIMER_LEFT_11_TM=59.983
PRIMER_RIGHT_11_TM=60.038
PRIMER_LEFT_11_GC_PERCENT=34.783
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.9000
PRIMER_RIGHT_11_END_STABILITY=9.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=533
PRIMER_PAIR_PENALTY_12=0.0551
PRIMER_LEFT_12_PENALTY=0.016975
PRIMER_RIGHT_12_PENALTY=0.038125
PRIMER_LEFT_12_SEQUENCE=ATCATATTTTGACAAGGAAGCGA
PRIMER_RIGHT_12_SEQUENCE=CTTTGTATATTTTCAAGCACGGC
PRIMER_LEFT_12=1155,23
PRIMER_RIGHT_12=1686,23
PRIMER_LEFT_12_TM=59.983
PRIMER_RIGHT_12_TM=60.038
PRIMER_LEFT_12_GC_PERCENT=34.783
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.9000
PRIMER_RIGHT_12_END_STABILITY=11.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=532
PRIMER_PAIR_PENALTY_13=0.0551
PRIMER_LEFT_13_PENALTY=0.016975
PRIMER_RIGHT_13_PENALTY=0.038125
PRIMER_LEFT_13_SEQUENCE=AAATGTATTCGAGCAAAGATCCA
PRIMER_RIGHT_13_SEQUENCE=GCTTTGTATATTTTCAAGCACGG
PRIMER_LEFT_13=1127,23
PRIMER_RIGHT_13=1687,23
PRIMER_LEFT_13_TM=59.983
PRIMER_RIGHT_13_TM=60.038
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=561
PRIMER_PAIR_PENALTY_14=0.0551
PRIMER_LEFT_14_PENALTY=0.016975
PRIMER_RIGHT_14_PENALTY=0.038125
PRIMER_LEFT_14_SEQUENCE=AAATGTATTCGAGCAAAGATCCA
PRIMER_RIGHT_14_SEQUENCE=CTTTGTATATTTTCAAGCACGGC
PRIMER_LEFT_14=1127,23
PRIMER_RIGHT_14=1686,23
PRIMER_LEFT_14_TM=59.983
PRIMER_RIGHT_14_TM=60.038
PRIMER_LEFT_14_GC_PERCENT=34.783
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=11.1000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=560
PRIMER_PAIR_PENALTY_15=0.0551
PRIMER_LEFT_15_PENALTY=0.016975
PRIMER_RIGHT_15_PENALTY=0.038125
PRIMER_LEFT_15_SEQUENCE=AATGTATTCGAGCAAAGATCCAA
PRIMER_RIGHT_15_SEQUENCE=GCTTTGTATATTTTCAAGCACGG
PRIMER_LEFT_15=1128,23
PRIMER_RIGHT_15=1687,23
PRIMER_LEFT_15_TM=59.983
PRIMER_RIGHT_15_TM=60.038
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=8.5000
PRIMER_RIGHT_15_END_STABILITY=9.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=560
PRIMER_PAIR_PENALTY_16=0.0551
PRIMER_LEFT_16_PENALTY=0.016975
PRIMER_RIGHT_16_PENALTY=0.038125
PRIMER_LEFT_16_SEQUENCE=AATGTATTCGAGCAAAGATCCAA
PRIMER_RIGHT_16_SEQUENCE=CTTTGTATATTTTCAAGCACGGC
PRIMER_LEFT_16=1128,23
PRIMER_RIGHT_16=1686,23
PRIMER_LEFT_16_TM=59.983
PRIMER_RIGHT_16_TM=60.038
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=11.1000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=559
PRIMER_PAIR_PENALTY_17=0.0557
PRIMER_LEFT_17_PENALTY=0.016975
PRIMER_RIGHT_17_PENALTY=0.038744
PRIMER_LEFT_17_SEQUENCE=ATCATATTTTGACAAGGAAGCGA
PRIMER_RIGHT_17_SEQUENCE=GCTGGAGGTGCTACTTCTTTACA
PRIMER_LEFT_17=1155,23
PRIMER_RIGHT_17=1665,23
PRIMER_LEFT_17_TM=59.983
PRIMER_RIGHT_17_TM=59.961
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=6.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=511
PRIMER_PAIR_PENALTY_18=0.0557
PRIMER_LEFT_18_PENALTY=0.016975
PRIMER_RIGHT_18_PENALTY=0.038744
PRIMER_LEFT_18_SEQUENCE=AAATGTATTCGAGCAAAGATCCA
PRIMER_RIGHT_18_SEQUENCE=GCTGGAGGTGCTACTTCTTTACA
PRIMER_LEFT_18=1127,23
PRIMER_RIGHT_18=1665,23
PRIMER_LEFT_18_TM=59.983
PRIMER_RIGHT_18_TM=59.961
PRIMER_LEFT_18_GC_PERCENT=34.783
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=6.0000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=539
PRIMER_PAIR_PENALTY_19=0.0557
PRIMER_LEFT_19_PENALTY=0.016975
PRIMER_RIGHT_19_PENALTY=0.038744
PRIMER_LEFT_19_SEQUENCE=AATGTATTCGAGCAAAGATCCAA
PRIMER_RIGHT_19_SEQUENCE=GCTGGAGGTGCTACTTCTTTACA
PRIMER_LEFT_19=1128,23
PRIMER_RIGHT_19=1665,23
PRIMER_LEFT_19_TM=59.983
PRIMER_RIGHT_19_TM=59.961
PRIMER_LEFT_19_GC_PERCENT=34.783
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.5000
PRIMER_RIGHT_19_END_STABILITY=6.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=538
=