PRIMER_SEQUENCE_ID=NM_005012
SEQUENCE=GAGCTGGAGCAGCCGCCACCGCCGCCGCCGAGGGAGCCCCGGGACGGCAGCCCCTGGGCGCAGGGTGCGCTGTTCTCGGAGTCCGACCCAGGGCGACTCACGCCCACTGGTGCGACCCGGACAGCCTGGGACTGACCCGCCGGCCCAGGCGAGGCTGCAGCCAGAGGGCTGGGAAGGGATCGCGCTCGCGGCATCCAGAGGCGGCCAGGCGGAGGCGAGGGAGCAGGTTAGAGGGACAAAGAGCTTTGCAGACGTCCCCGGCGTCCTGCGAGCGCCAGCGGCCGGGACGAGGCGGCCGGGAGCCCGGGAAGAGCCCGTGGATGTTCTGCGCGCGGCCTGGGAGCCGCCGCCGCCGCCGCCTCAGCGAGAGGAGGAATGCACCGGCCGCGCCGCCGCGGGACGCGCCCGCCGCTCCTGGCGCTGCTGGCCGCGCTGCTGCTGGCCGCACGCGGGGCTGCTGCCCAAGAAACAGAGCTGTCAGTCAGTGCTGAATTAGTGCCTACCTCATCATGGAACATCTCAAGTGAACTCAACAAAGATTCTTACCTGACCCTTGATGAACCAATGAATAACATCACCACGTCTCTGGGCCAGACAGCAGAACTGCACTGCAAAGTCTCTGGGAATCCACCTCCCACCATCCGCTGGTTCAAAAATGATGCTCCTGTGGTCCAGGAGCCCCGGAGGCTCTCCTTTCGGTCCACCATCTATGGCTCTCGGCTGCGGATTAGAAACCTCGACACCACAGACACAGGCTACTTCCAGTGCGTGGCAACAAACGGCAAGGAGGTGGTTTCTTCCACTGGAGTCTTGTTTGTCAAGTTTGGCCCCCCTCCCACTGCAAGTCCAGGATACTCAGATGAGTATGAAGAAGATGGATTCTGTCAGCCATACAGAGGGATTGCATGTGCAAGATTTATTGGCAACCGCACCGTCTATATGGAGTCTTTGCACATGCAAGGGGAAATAGAAAATCAGATCACAGCTGCCTTCACTATGATTGGCACTTCCAGTCACTTATCTGATAAGTGTTCTCAGTTCGCCATTCCTTCCCTGTGCCACTATGCCTTCCCGTACTGCGATGAAACTTCATCCGTCCCAAAGCCCCGTGACTTGTGTCGCGATGAATGTGAAATCCTGGAGAATGTCCTGTGTCAAACAGAGTACATTTTTGCAAGATCAAATCCCATGATTCTGATGAGGCTGAAACTGCCAAACTGTGAAGATCTCCCCCAGCCAGAGAGCCCAGAAGCTGCGAACTGTATCCGGATTGGAATTCCCATGGCAGATCCTATAAATAAAAATCACAAGTGTTATAACAGCACAGGTGTGGACTACCGGGGGACCGTCAGTGTGACCAAATCAGGGCGCCAGTGCCAGCCATGGAATTCCCAGTATCCCCACACACACACTTTCACCGCCCTTCGTTTCCCAGAGCTGAATGGAGGCCATTCCTACTGCCGCAACCCAGGGAATCAAAAGGAAGCTCCCTGGTGCTTCACCTTGGATGAAAACTTTAAGTCTGATCTGTGTGACATCCCAGCTTGCGATTCAAAGGATTCCAAGGAGAAGAATAAAATGGAAATCCTGTACATACTAGTGCCAAGTGTGGCCATTCCCCTGGCCATTGCTTTACTCTTCTTCTTCATTTGCGTCTGTCGGAATAACCAGAAGTCATCGTCGGCACCAGTCCAGAGGCAACCAAAACACGTCAGAGGTCAAAATGTGGAGATGTCAATGCTGAATGCATATAAACCCAAGAGCAAGGCTAAAGAGCTACCTCTTTCTGCTGTACGCTTTATGGAAGAATTGGGTGAGTGTGCCTTTGGAAAAATCTATAAAGGCCATCTCTATCTCCCAGGCATGGACCATGCTCAGCTGGTTGCTATCAAGACCTTGAAAGACTATAACAACCCCCAGCAATGGATGGAATTTCAACAAGAAGCCTCCCTAATGGCAGAACTGCACCACCCCAATATTGTCTGCCTTCTAGGTGCCGTCACTCAGGAACAACCTGTGTGCATGCTTTTTGAGTATATTAATCAGGGGGATCTCCATGAGTTCCTCATCATGAGATCCCCACACTCTGATGTTGGCTGCAGCAGTGATGAAGATGGGACTGTGAAATCCAGCCTGGACCACGGAGATTTTCTGCACATTGCAATTCAGATTGCAGCTGGCATGGAATACCTGTCTAGTCACTTCTTTGTCCACAAGGACCTTGCAGCTCGCAATATTTTAATCGGAGAGCAACTTCATGTAAAGATTTCAGACTTGGGGCTTTCCAGAGAAATTTACTCCGCTGATTACTACAGGGTCCAGAGTAAGTCCTTGCTGCCCATTCGCTGGATGCCCCCTGAAGCCATCATGTATGGCAAATTCTCTTCTGATTCAGATATCTGGTCCTTTGGGGTTGTCTTGTGGGAGATTTTCAGTTTTGGACTCCAGCCATATTATGGATTCAGTAACCAGGAAGTGATTGAGATGGTGAGAAAACGGCAGCTCTTACCATGCTCTGAAGACTGCCCACCCAGAATGTACAGCCTCATGACAGAGTGCTGGAATGAGATTCCTTCTAGGAGACCAAGATTTAAAGATATTCACGTCCGGCTTCGGTCCTGGGAGGGACTCTCAAGTCACACAAGCTCTACTACTCCTTCAGGGGGAAATGCCACCACACAGACAACCTCCCTCAGTGCCAGCCCAGTGAGTAATCTCAGTAACCCCAGATATCCTAATTACATGTTCCCGAGCCAGGGTATTACACCACAGGGCCAGATTGCTGGTTTCATTGGCCCGCCAATACCTCAGAACCAGCGATTCATTCCCATCAATGGATACCCAATACCTCCTGGATATGCAGCGTTTCCAGCTGCCCACTACCAGCCAACAGGTCCTCCCAGAGTGATTCAGCACTGCCCACCTCCCAAGAGTCGGTCCCCAAGCAGTGCCAGTGGGTCGACTAGCACTGGCCATGTGACTAGCTTGCCCTCATCAGGATCCAATCAGGAAGCAAATATTCCTTTACTACCACACATGTCAATTCCAAATCATCCTGGTGGAATGGGTATCACCGTTTTTGGCAACAAATCTCAAAAACCCTACAAAATTGACTCAAAGCAAGCATCTTTACTAGGAGACGCCAATATTCATGGACACACCGAATCTATGATTTCTGCAGAACTGTAAAATGCACAACTTTTGTAAATGTGGTATACAGGACAAACTAGACGGCCGTAGAAAAGATTTATATTCAAATGTTTTTATTAAAGTAAGGTTCTCATTTAGCAGACATCGCAACAAGTACCTTCTGTGAAGTTTCACTGTGTCTTACCAAGCAGGACAGACACTCGGCCAG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 38943, GC content failed 2562, low tm 14490, high tm 13558, high any compl 2, high end compl 15, high repeat similarity 23, long poly-x seq 21, ok 8272
PRIMER_RIGHT_EXPLAIN=considered 38943, GC content failed 2039, low tm 15064, high tm 13341, high any compl 1, high end compl 6, high repeat similarity 12, long poly-x seq 21, ok 8459
PRIMER_PAIR_EXPLAIN=considered 802, unacceptable product size 737, high end compl 4, ok 61
PRIMER_PAIR_PENALTY=0.0392
PRIMER_LEFT_PENALTY=0.017590
PRIMER_RIGHT_PENALTY=0.021648
PRIMER_LEFT_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_SEQUENCE=CAAAACTGAAAATCTCCCACAAG
PRIMER_LEFT=2259,23
PRIMER_RIGHT=2439,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.0436
PRIMER_LEFT_1_PENALTY=0.017590
PRIMER_RIGHT_1_PENALTY=0.026007
PRIMER_LEFT_1_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_1_SEQUENCE=ACTGAAAATCTCCCACAAGACAA
PRIMER_LEFT_1=2259,23
PRIMER_RIGHT_1=2435,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.0436
PRIMER_LEFT_2_PENALTY=0.017590
PRIMER_RIGHT_2_PENALTY=0.026007
PRIMER_LEFT_2_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_2_SEQUENCE=AACTGAAAATCTCCCACAAGACA
PRIMER_LEFT_2=2259,23
PRIMER_RIGHT_2=2436,23
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.0777
PRIMER_LEFT_3_PENALTY=0.034654
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=CCGTCTATATGGAGTCTTTGCAC
PRIMER_RIGHT_3_SEQUENCE=ACATTTTGACCTCTGACGTGTTT
PRIMER_LEFT_3=931,23
PRIMER_RIGHT_3=1726,23
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=796
PRIMER_PAIR_PENALTY_4=0.0777
PRIMER_LEFT_4_PENALTY=0.034654
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=CACCGTCTATATGGAGTCTTTGC
PRIMER_RIGHT_4_SEQUENCE=ACATTTTGACCTCTGACGTGTTT
PRIMER_LEFT_4=929,23
PRIMER_RIGHT_4=1726,23
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=798
PRIMER_PAIR_PENALTY_5=0.0777
PRIMER_LEFT_5_PENALTY=0.034654
PRIMER_RIGHT_5_PENALTY=0.043085
PRIMER_LEFT_5_SEQUENCE=GCACCGTCTATATGGAGTCTTTG
PRIMER_RIGHT_5_SEQUENCE=ACATTTTGACCTCTGACGTGTTT
PRIMER_LEFT_5=928,23
PRIMER_RIGHT_5=1726,23
PRIMER_LEFT_5_TM=60.035
PRIMER_RIGHT_5_TM=59.957
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=799
PRIMER_PAIR_PENALTY_6=0.0816
PRIMER_LEFT_6_PENALTY=0.046971
PRIMER_RIGHT_6_PENALTY=0.034654
PRIMER_LEFT_6_SEQUENCE=ATTAGAAACCTCGACACCACAGA
PRIMER_RIGHT_6_SEQUENCE=TGCAAAGACTCCATATAGACGGT
PRIMER_LEFT_6=726,23
PRIMER_RIGHT_6=952,23
PRIMER_LEFT_6_TM=60.047
PRIMER_RIGHT_6_TM=60.035
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.3000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=227
PRIMER_PAIR_PENALTY_7=0.0816
PRIMER_LEFT_7_PENALTY=0.046971
PRIMER_RIGHT_7_PENALTY=0.034654
PRIMER_LEFT_7_SEQUENCE=GATTAGAAACCTCGACACCACAG
PRIMER_RIGHT_7_SEQUENCE=TGCAAAGACTCCATATAGACGGT
PRIMER_LEFT_7=725,23
PRIMER_RIGHT_7=952,23
PRIMER_LEFT_7_TM=60.047
PRIMER_RIGHT_7_TM=60.035
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=9.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=228
PRIMER_PAIR_PENALTY_8=0.0957
PRIMER_LEFT_8_PENALTY=0.061028
PRIMER_RIGHT_8_PENALTY=0.034654
PRIMER_LEFT_8_SEQUENCE=GGAGTCTTGTTTGTCAAGTTTGG
PRIMER_RIGHT_8_SEQUENCE=TGCAAAGACTCCATATAGACGGT
PRIMER_LEFT_8=804,23
PRIMER_RIGHT_8=952,23
PRIMER_LEFT_8_TM=60.061
PRIMER_RIGHT_8_TM=60.035
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MARINER1 Human mariner
PRIMER_LEFT_8_END_STABILITY=8.8000
PRIMER_RIGHT_8_END_STABILITY=9.3000
PRIMER_PAIR_8_COMPL_ANY=8.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=149
PRIMER_PAIR_PENALTY_9=0.1028
PRIMER_LEFT_9_PENALTY=0.068149
PRIMER_RIGHT_9_PENALTY=0.034654
PRIMER_LEFT_9_SEQUENCE=GACCCTTGATGAACCAATGAATA
PRIMER_RIGHT_9_SEQUENCE=TGCAAAGACTCCATATAGACGGT
PRIMER_LEFT_9=548,23
PRIMER_RIGHT_9=952,23
PRIMER_LEFT_9_TM=60.068
PRIMER_RIGHT_9_TM=60.035
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.9000
PRIMER_RIGHT_9_END_STABILITY=9.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=405
PRIMER_PAIR_PENALTY_10=0.1041
PRIMER_LEFT_10_PENALTY=0.061028
PRIMER_RIGHT_10_PENALTY=0.043085
PRIMER_LEFT_10_SEQUENCE=GGAGTCTTGTTTGTCAAGTTTGG
PRIMER_RIGHT_10_SEQUENCE=ACATTTTGACCTCTGACGTGTTT
PRIMER_LEFT_10=804,23
PRIMER_RIGHT_10=1726,23
PRIMER_LEFT_10_TM=60.061
PRIMER_RIGHT_10_TM=59.957
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=8.8000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=923
PRIMER_PAIR_PENALTY_11=0.1062
PRIMER_LEFT_11_PENALTY=0.017590
PRIMER_RIGHT_11_PENALTY=0.088643
PRIMER_LEFT_11_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_11_SEQUENCE=TGAATCAGAAGAGAATTTGCCAT
PRIMER_LEFT_11=2259,23
PRIMER_RIGHT_11=2393,23
PRIMER_LEFT_11_TM=60.018
PRIMER_RIGHT_11_TM=60.089
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=9.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=135
PRIMER_PAIR_PENALTY_12=0.1151
PRIMER_LEFT_12_PENALTY=0.068149
PRIMER_RIGHT_12_PENALTY=0.046971
PRIMER_LEFT_12_SEQUENCE=GACCCTTGATGAACCAATGAATA
PRIMER_RIGHT_12_SEQUENCE=TCTGTGGTGTCGAGGTTTCTAAT
PRIMER_LEFT_12=548,23
PRIMER_RIGHT_12=748,23
PRIMER_LEFT_12_TM=60.068
PRIMER_RIGHT_12_TM=60.047
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.9000
PRIMER_RIGHT_12_END_STABILITY=5.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=201
PRIMER_PAIR_PENALTY_13=0.1266
PRIMER_LEFT_13_PENALTY=0.017590
PRIMER_RIGHT_13_PENALTY=0.109043
PRIMER_LEFT_13_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_13_SEQUENCE=TCTGCAGAAATCATAGATTCGGT
PRIMER_LEFT_13=2259,23
PRIMER_RIGHT_13=3181,23
PRIMER_LEFT_13_TM=60.018
PRIMER_RIGHT_13_TM=60.109
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=8.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MER39 a consensus
PRIMER_LEFT_13_END_STABILITY=10.9000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=923
PRIMER_PAIR_PENALTY_14=0.1350
PRIMER_LEFT_14_PENALTY=0.026007
PRIMER_RIGHT_14_PENALTY=0.109043
PRIMER_LEFT_14_SEQUENCE=TGTCTTGTGGGAGATTTTCAGTT
PRIMER_RIGHT_14_SEQUENCE=TCTGCAGAAATCATAGATTCGGT
PRIMER_LEFT_14=2414,23
PRIMER_RIGHT_14=3181,23
PRIMER_LEFT_14_TM=60.026
PRIMER_RIGHT_14_TM=60.109
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER44C Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=9.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=768
PRIMER_PAIR_PENALTY_15=0.1350
PRIMER_LEFT_15_PENALTY=0.026007
PRIMER_RIGHT_15_PENALTY=0.109043
PRIMER_LEFT_15_SEQUENCE=TTGTCTTGTGGGAGATTTTCAGT
PRIMER_RIGHT_15_SEQUENCE=TCTGCAGAAATCATAGATTCGGT
PRIMER_LEFT_15=2413,23
PRIMER_RIGHT_15=3181,23
PRIMER_LEFT_15_TM=60.026
PRIMER_RIGHT_15_TM=60.109
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=9.6000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=769
PRIMER_PAIR_PENALTY_16=0.1350
PRIMER_LEFT_16_PENALTY=0.026007
PRIMER_RIGHT_16_PENALTY=0.109043
PRIMER_LEFT_16_SEQUENCE=GTTGTCTTGTGGGAGATTTTCAG
PRIMER_RIGHT_16_SEQUENCE=TCTGCAGAAATCATAGATTCGGT
PRIMER_LEFT_16=2412,23
PRIMER_RIGHT_16=3181,23
PRIMER_LEFT_16_TM=60.026
PRIMER_RIGHT_16_TM=60.109
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=8.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=770
PRIMER_PAIR_PENALTY_17=0.1356
PRIMER_LEFT_17_PENALTY=0.109631
PRIMER_RIGHT_17_PENALTY=0.026007
PRIMER_LEFT_17_SEQUENCE=GGTCAAAATGTGGAGATGTCAAT
PRIMER_RIGHT_17_SEQUENCE=ACTGAAAATCTCCCACAAGACAA
PRIMER_LEFT_17=1716,23
PRIMER_RIGHT_17=2435,23
PRIMER_LEFT_17_TM=60.110
PRIMER_RIGHT_17_TM=60.026
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=7.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=720
PRIMER_PAIR_PENALTY_18=0.1356
PRIMER_LEFT_18_PENALTY=0.109631
PRIMER_RIGHT_18_PENALTY=0.026007
PRIMER_LEFT_18_SEQUENCE=GGTCAAAATGTGGAGATGTCAAT
PRIMER_RIGHT_18_SEQUENCE=AACTGAAAATCTCCCACAAGACA
PRIMER_LEFT_18=1716,23
PRIMER_RIGHT_18=2436,23
PRIMER_LEFT_18_TM=60.110
PRIMER_RIGHT_18_TM=60.026
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=721
PRIMER_PAIR_PENALTY_19=0.1536
PRIMER_LEFT_19_PENALTY=0.017590
PRIMER_RIGHT_19_PENALTY=0.136051
PRIMER_LEFT_19_SEQUENCE=GTAAAGATTTCAGACTTGGGGCT
PRIMER_RIGHT_19_SEQUENCE=CTAGAAGGAATCTCATTCCAGCA
PRIMER_LEFT_19=2259,23
PRIMER_RIGHT_19=2578,23
PRIMER_LEFT_19_TM=60.018
PRIMER_RIGHT_19_TM=59.864
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=10.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=320
=