PRIMER_SEQUENCE_ID=NM_005211
SEQUENCE=GGCTTCAGGAAGGGCAGACAGAGTGTCCAAAAGCGTGAGAGCACGAAGTGAGGAGAAGGTGGAGAAGAGAGAAGAGGAAGAGGAAGAGGAAGAGAGGAAGCGGAGGGAACTGCGGCCAGGCTAAAAGGGGAAGAAGAGGATCAGCCCAAGGAGGAGGAAGAGGAAAACAAGACAAACAGCCAGTGCAGAGGAGAGGAACGTGTGTCCAGTGTCCCGATCCCTGCGGAGCTAGTAGCTGAGAGCTCTGTGCCCTGGGCACCTTGCAGCCCTGCACCTGCCTGCCACTTCCCCACCGAGGCCATGGGCCCAGGAGTTCTGCTGCTCCTGCTGGTGGCCACAGCTTGGCATGGTCAGGGAATCCCAGTGATAGAGCCCAGTGTCCCCGAGCTGGTCGTGAAGCCAGGAGCAACGGTGACCTTGCGATGTGTGGGCAATGGCAGCGTGGAATGGGATGGCCCCGCATCACCTCACTGGACCCTGTACTCTGATGGCTCCAGCAGCATCCTCAGCACCAACAACGCTACCTTCCAAAACACGGGGACCTATCGCTGCACTGAGCCTGGAGACCCCCTGGGAGGCAGCGCCGCCATCCACCTCTATGTCAAAGACCCTGCCCGGCCCTGGAACGTGCTAGCACAGGAGGTGGTCGTGTTCGAGGACCAGGACGCACTACTGCCCTGTCTGCTCACAGACCCGGTGCTGGAAGCAGGCGTCTCGCTGGTGCGTGTGCGTGGCCGGCCCCTCATGCGCCACACCAACTACTCCTTCTCGCCCTGGCATGGCTTCACCATCCACAGGGCCAAGTTCATTCAGAGCCAGGACTATCAATGCAGTGCCCTGATGGGTGGCAGGAAGGTGATGTCCATCAGCATCCGGCTGAAAGTGCAGAAAGTCATCCCAGGGCCCCCAGCCTTGACACTGGTGCCTGCAGAGCTGGTGCGGATTCGAGGGGAGGCTGCCCAGATCGTGTGCTCAGCCAGCAGCGTTGATGTTAACTTTGATGTCTTCCTCCAACACAACAACACTAAGCTCGCAATCCCTCAACAATCTGACTTTCATAATAACCGTTACCAAAAAGTCCTGACCCTCAACCTCGATCAAGTAGATTTCCAACATGCCGGCAACTACTCCTGCGTGGCCAGCAACGTGCAGGGCAAGCACTCCACCTCCATGTTCTTCCGGGTGGTAGAGAGTGCCTACTTGAACTTGAGCTCTGAGCAGAACCTCATCCAGGAGGTGACCGTGGGGGAGGGGCTCAACCTCAAAGTCATGGTGGAGGCCTACCCAGGCCTGCAAGGTTTTAACTGGACCTACCTGGGACCCTTTTCTGACCACCAGCCTGAGCCCAAGCTTGCTAATGCTACCACCAAGGACACATACAGGCACACCTTCACCCTCTCTCTGCCCCGCCTGAAGCCCTCTGAGGCTGGCCGCTACTCCTTCCTGGCCAGAAACCCAGGAGGCTGGAGAGCTCTGACGTTTGAGCTCACCCTTCGATACCCCCCAGAGGTAAGCGTCATATGGACATTCATCAACGGCTCTGGCACCCTTTTGTGTGCTGCCTCTGGGTACCCCCAGCCCAACGTGACATGGCTGCAGTGCAGTGGCCACACTGATAGGTGTGATGAGGCCCAAGTGCTGCAGGTCTGGGATGACCCATACCCTGAGGTCCTGAGCCAGGAGCCCTTCCACAAGGTGACGGTGCAGAGCCTGCTGACTGTTGAGACCTTAGAGCACAACCAAACCTACGAGTGCAGGGCCCACAACAGCGTGGGGAGTGGCTCCTGGGCCTTCATACCCATCTCTGCAGGAGCCCACACGCATCCCCCGGATGAGTTCCTCTTCACACCAGTGGTGGTCGCCTGCATGTCCATCATGGCCTTGCTGCTGCTGCTGCTCCTGCTGCTATTGTACAAGTATAAGCAGAAGCCCAAGTACCAGGTCCGCTGGAAGATCATCGAGAGCTATGAGGGCAACAGTTATACTTTCATCGACCCCACGCAGCTGCCTTACAACGAGAAGTGGGAGTTCCCCCGGAACAACCTGCAGTTTGGTAAGACCCTCGGAGCTGGAGCCTTTGGGAAGGTGGTGGAGGCCACGGCCTTTGGTCTGGGCAAGGAGGATGCTGTCCTGAAGGTGGCTGTGAAGATGCTGAAGTCCACGGCCCATGCTGATGAGAAGGAGGCCCTCATGTCCGAGCTGAAGATCATGAGCCACCTGGGCCAGCACGAGAACATCGTCAACCTTCTGGGAGCCTGTACCCATGGAGGCCCTGTACTGGTCATCACGGAGTACTGTTGCTATGGCGACCTGCTCAACTTTCTGCGAAGGAAGGCTGAGGCCATGCTGGGACCCAGCCTGAGCCCCGGCCAGGACCCCGAGGGAGGCGTCGACTATAAGAACATCCACCTCGAGAAGAAATATGTCCGCAGGGACAGTGGCTTCTCCAGCCAGGGTGTGGACACCTATGTGGAGATGAGGCCTGTCTCCACTTCTTCAAATGACTCCTTCTCTGAGCAAGACCTGGACAAGGAGGATGGACGGCCCCTGGAGCTCCGGGACCTGCTTCACTTCTCCAGCCAAGTAGCCCAGGGCATGGCCTTCCTCGCTTCCAAGAATTGCATCCACCGGGACGTGGCAGCGCGTAACGTGCTGTTGACCAATGGTCATGTGGCCAAGATTGGGGACTTCGGGCTGGCTAGGGACATCATGAATGACTCCAACTACATTGTCAAGGGCAATGCCCGCCTGCCTGTGAAGTGGATGGCCCCAGAGAGCATCTTTGACTGTGTCTACACGGTTCAGAGCGACGTCTGGTCCTATGGCATCCTCCTCTGGGAGATCTTCTCACTTGGGCTGAATCCCTACCCTGGCATCCTGGTGAACAGCAAGTTCTATAAACTGGTGAAGGATGGATACCAAATGGCCCAGCCTGCATTTGCCCCAAAGAATATATACAGCATCATGCAGGCCTGCTGGGCCTTGGAGCCCACCCACAGACCCACCTTCCAGCAGATCTGCTCCTTCCTTCAGGAGCAGGCCCAAGAGGACAGGAGAGAGCGGGACTATACCAATCTGCCGAGCAGCAGCAGAAGCGGTGGCAGCGGCAGCAGCAGCAGTGAGCTGGAGGAGGAGAGCTCTAGTGAGCACCTGACCTGCTGCGAGCAAGGGGATATCGCCCAGCCCTTGCTGCAGCCCAACAACTATCAGTTCTGCTGAGGAGTTGACGACAGGGAGTACCACTCTCCCCTCCTCCAAACTTCAACTCCTCCATGGATGGGGCGACACGGGGAGAACATACAAACTCTGCCTTCGGTCATTTCACTCAACAGCTCGGCCCAGCTCTGAAACTTGGGAAGGTGAGGGATTCAGGGGAGGTCAGAGGATCCCACTTCCTGAGCATGGGCCATCACTGCCAGTCAGGGGCTGGGGGCTGAGCCCTCACCCCCCGCCTCCCCTACTGTTCTCATGGTGTTGGCCTCGTGTTTGCTATGCCAACTAGTAGAACCTTCTTTCCTAATCCCCTTATCTTCATGGAAATGGACTGACTTTATGCCTATGAAGTCCCCAGGAGCTACACTGATACTGAGAAAACCAGGCTCTTTGGGGCTAGACAGACTGGCAGAGAGTGAGATCTCCCTCTCTGAGAGGAGCAGCAGATGCTCACAGACCACACTCAGCTCAGGCCCCTTGGAGCAGGATGGCTCCTCTAAGAATCTCACAGGACCTCTTAGTCTCTGCCCTATACGCCGCCTTCACTCCACAGCCTCACCCCTCCCACCCCCATACTGGTACTGCTGTAATGAGCCAAGTGGCAGCTAAAAGTTGGGGGTGTTCTGCCCAGTCCCGTCATTCTGGGCTAGAAGGCAGGGGACCTTGGCATTGGCTGGCCACACCAAGCAGGAAGCACAAACTCCCCCAAGCTGACTCATCCTAACTAACAGTCACGCCGTGGGATGTCTCTGTCCACATTAAACTAACAGCATTAATGC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 46415, GC content failed 337, low tm 12868, high tm 23547, high any compl 7, high end compl 16, high repeat similarity 47, long poly-x seq 42, ok 9551
PRIMER_RIGHT_EXPLAIN=considered 46417, GC content failed 341, low tm 12866, high tm 23599, high any compl 1, high end compl 8, high repeat similarity 6, long poly-x seq 40, ok 9556
PRIMER_PAIR_EXPLAIN=considered 1108, unacceptable product size 1043, high end compl 1, ok 64
PRIMER_PAIR_PENALTY=0.0356
PRIMER_LEFT_PENALTY=0.009608
PRIMER_RIGHT_PENALTY=0.026007
PRIMER_LEFT_SEQUENCE=TCTTCATGGAAATGGACTGACTT
PRIMER_RIGHT_SEQUENCE=CTGTGAGATTCTTAGAGGAGCCA
PRIMER_LEFT=3528,23
PRIMER_RIGHT=3723,23
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.0375
PRIMER_LEFT_1_PENALTY=0.025930
PRIMER_RIGHT_1_PENALTY=0.011619
PRIMER_LEFT_1_SEQUENCE=ACTGGTCATCACGGAGTACTGTT
PRIMER_RIGHT_1_SEQUENCE=GCTCAGAGAAGGAGTCATTTGAA
PRIMER_LEFT_1=2276,23
PRIMER_RIGHT_1=2517,23
PRIMER_LEFT_1_TM=59.974
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.0386
PRIMER_LEFT_2_PENALTY=0.026932
PRIMER_RIGHT_2_PENALTY=0.011619
PRIMER_LEFT_2_SEQUENCE=CATCCACCTCGAGAAGAAATATG
PRIMER_RIGHT_2_SEQUENCE=GCTCAGAGAAGGAGTCATTTGAA
PRIMER_LEFT_2=2402,23
PRIMER_RIGHT_2=2517,23
PRIMER_LEFT_2_TM=59.973
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.0529
PRIMER_LEFT_3_PENALTY=0.025930
PRIMER_RIGHT_3_PENALTY=0.026932
PRIMER_LEFT_3_SEQUENCE=ACTGGTCATCACGGAGTACTGTT
PRIMER_RIGHT_3_SEQUENCE=CATATTTCTTCTCGAGGTGGATG
PRIMER_LEFT_3=2276,23
PRIMER_RIGHT_3=2424,23
PRIMER_LEFT_3_TM=59.974
PRIMER_RIGHT_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.0541
PRIMER_LEFT_4_PENALTY=0.025930
PRIMER_RIGHT_4_PENALTY=0.028128
PRIMER_LEFT_4_SEQUENCE=ACTGGTCATCACGGAGTACTGTT
PRIMER_RIGHT_4_SEQUENCE=CGAGGTGGATGTTCTTATAGTCG
PRIMER_LEFT_4=2276,23
PRIMER_RIGHT_4=2412,23
PRIMER_LEFT_4_TM=59.974
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
PRIMER_PAIR_PENALTY_5=0.0832
PRIMER_LEFT_5_PENALTY=0.009608
PRIMER_RIGHT_5_PENALTY=0.073619
PRIMER_LEFT_5_SEQUENCE=TCCCTCAACAATCTGACTTTCAT
PRIMER_RIGHT_5_SEQUENCE=TAACTGTTGCCCTCATAGCTCTC
PRIMER_LEFT_5=1036,23
PRIMER_RIGHT_5=1981,23
PRIMER_LEFT_5_TM=59.990
PRIMER_RIGHT_5_TM=59.926
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=946
PRIMER_PAIR_PENALTY_6=0.0832
PRIMER_LEFT_6_PENALTY=0.009608
PRIMER_RIGHT_6_PENALTY=0.073619
PRIMER_LEFT_6_SEQUENCE=ATCCCTCAACAATCTGACTTTCA
PRIMER_RIGHT_6_SEQUENCE=TAACTGTTGCCCTCATAGCTCTC
PRIMER_LEFT_6=1035,23
PRIMER_RIGHT_6=1981,23
PRIMER_LEFT_6_TM=59.990
PRIMER_RIGHT_6_TM=59.926
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=947
PRIMER_PAIR_PENALTY_7=0.0852
PRIMER_LEFT_7_PENALTY=0.073619
PRIMER_RIGHT_7_PENALTY=0.011619
PRIMER_LEFT_7_SEQUENCE=GAGAGCTATGAGGGCAACAGTTA
PRIMER_RIGHT_7_SEQUENCE=GCTCAGAGAAGGAGTCATTTGAA
PRIMER_LEFT_7=1959,23
PRIMER_RIGHT_7=2517,23
PRIMER_LEFT_7_TM=59.926
PRIMER_RIGHT_7_TM=60.012
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=559
PRIMER_PAIR_PENALTY_8=0.0914
PRIMER_LEFT_8_PENALTY=0.065377
PRIMER_RIGHT_8_PENALTY=0.026007
PRIMER_LEFT_8_SEQUENCE=TACTGAGAAAACCAGGCTCTTTG
PRIMER_RIGHT_8_SEQUENCE=CTGTGAGATTCTTAGAGGAGCCA
PRIMER_LEFT_8=3583,23
PRIMER_RIGHT_8=3723,23
PRIMER_LEFT_8_TM=59.935
PRIMER_RIGHT_8_TM=60.026
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=141
PRIMER_PAIR_PENALTY_9=0.0971
PRIMER_LEFT_9_PENALTY=0.011619
PRIMER_RIGHT_9_PENALTY=0.085505
PRIMER_LEFT_9_SEQUENCE=TTCAAATGACTCCTTCTCTGAGC
PRIMER_RIGHT_9_SEQUENCE=CCCTTGACAATGTAGTTGGAGTC
PRIMER_LEFT_9=2495,23
PRIMER_RIGHT_9=2737,23
PRIMER_LEFT_9_TM=60.012
PRIMER_RIGHT_9_TM=59.914
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=243
PRIMER_PAIR_PENALTY_10=0.0995
PRIMER_LEFT_10_PENALTY=0.073485
PRIMER_RIGHT_10_PENALTY=0.026007
PRIMER_LEFT_10_SEQUENCE=GACAGACTGGCAGAGAGTGAGAT
PRIMER_RIGHT_10_SEQUENCE=CTGTGAGATTCTTAGAGGAGCCA
PRIMER_LEFT_10=3612,23
PRIMER_RIGHT_10=3723,23
PRIMER_LEFT_10_TM=60.073
PRIMER_RIGHT_10_TM=60.026
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.3000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=112
PRIMER_PAIR_PENALTY_11=0.0996
PRIMER_LEFT_11_PENALTY=0.026007
PRIMER_RIGHT_11_PENALTY=0.073619
PRIMER_LEFT_11_SEQUENCE=AACTTTGATGTCTTCCTCCAACA
PRIMER_RIGHT_11_SEQUENCE=TAACTGTTGCCCTCATAGCTCTC
PRIMER_LEFT_11=993,23
PRIMER_RIGHT_11=1981,23
PRIMER_LEFT_11_TM=60.026
PRIMER_RIGHT_11_TM=59.926
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER44C Nonautonomous DNA transposon
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=6.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=989
PRIMER_PAIR_PENALTY_12=0.1006
PRIMER_LEFT_12_PENALTY=0.073619
PRIMER_RIGHT_12_PENALTY=0.026932
PRIMER_LEFT_12_SEQUENCE=GAGAGCTATGAGGGCAACAGTTA
PRIMER_RIGHT_12_SEQUENCE=CATATTTCTTCTCGAGGTGGATG
PRIMER_LEFT_12=1959,23
PRIMER_RIGHT_12=2424,23
PRIMER_LEFT_12_TM=59.926
PRIMER_RIGHT_12_TM=59.973
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.7000
PRIMER_RIGHT_12_END_STABILITY=8.1000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=466
PRIMER_PAIR_PENALTY_13=0.1017
PRIMER_LEFT_13_PENALTY=0.073619
PRIMER_RIGHT_13_PENALTY=0.028128
PRIMER_LEFT_13_SEQUENCE=GAGAGCTATGAGGGCAACAGTTA
PRIMER_RIGHT_13_SEQUENCE=CGAGGTGGATGTTCTTATAGTCG
PRIMER_LEFT_13=1959,23
PRIMER_RIGHT_13=2412,23
PRIMER_LEFT_13_TM=59.926
PRIMER_RIGHT_13_TM=60.028
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=454
PRIMER_PAIR_PENALTY_14=0.1060
PRIMER_LEFT_14_PENALTY=0.094334
PRIMER_RIGHT_14_PENALTY=0.011619
PRIMER_LEFT_14_SEQUENCE=CGTCGACTATAAGAACATCCACC
PRIMER_RIGHT_14_SEQUENCE=GCTCAGAGAAGGAGTCATTTGAA
PRIMER_LEFT_14=2387,23
PRIMER_RIGHT_14=2517,23
PRIMER_LEFT_14_TM=59.906
PRIMER_RIGHT_14_TM=60.012
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=131
PRIMER_PAIR_PENALTY_15=0.1114
PRIMER_LEFT_15_PENALTY=0.025930
PRIMER_RIGHT_15_PENALTY=0.085505
PRIMER_LEFT_15_SEQUENCE=ACTGGTCATCACGGAGTACTGTT
PRIMER_RIGHT_15_SEQUENCE=CCCTTGACAATGTAGTTGGAGTC
PRIMER_LEFT_15=2276,23
PRIMER_RIGHT_15=2737,23
PRIMER_LEFT_15_TM=59.974
PRIMER_RIGHT_15_TM=59.914
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=6.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=462
PRIMER_PAIR_PENALTY_16=0.1124
PRIMER_LEFT_16_PENALTY=0.026932
PRIMER_RIGHT_16_PENALTY=0.085505
PRIMER_LEFT_16_SEQUENCE=CATCCACCTCGAGAAGAAATATG
PRIMER_RIGHT_16_SEQUENCE=CCCTTGACAATGTAGTTGGAGTC
PRIMER_LEFT_16=2402,23
PRIMER_RIGHT_16=2737,23
PRIMER_LEFT_16_TM=59.973
PRIMER_RIGHT_16_TM=59.914
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=5.8000
PRIMER_RIGHT_16_END_STABILITY=6.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=336
PRIMER_PAIR_PENALTY_17=0.1317
PRIMER_LEFT_17_PENALTY=0.122120
PRIMER_RIGHT_17_PENALTY=0.009608
PRIMER_LEFT_17_SEQUENCE=CATCCACCTCTATGTCAAAGACC
PRIMER_RIGHT_17_SEQUENCE=ATGAAAGTCAGATTGTTGAGGGA
PRIMER_LEFT_17=587,23
PRIMER_RIGHT_17=1058,23
PRIMER_LEFT_17_TM=59.878
PRIMER_RIGHT_17_TM=59.990
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.6000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=472
PRIMER_PAIR_PENALTY_18=0.1317
PRIMER_LEFT_18_PENALTY=0.122120
PRIMER_RIGHT_18_PENALTY=0.009608
PRIMER_LEFT_18_SEQUENCE=CATCCACCTCTATGTCAAAGACC
PRIMER_RIGHT_18_SEQUENCE=TGAAAGTCAGATTGTTGAGGGAT
PRIMER_LEFT_18=587,23
PRIMER_RIGHT_18=1057,23
PRIMER_LEFT_18_TM=59.878
PRIMER_RIGHT_18_TM=59.990
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.6000
PRIMER_RIGHT_18_END_STABILITY=9.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=471
PRIMER_PAIR_PENALTY_19=0.1540
PRIMER_LEFT_19_PENALTY=0.080331
PRIMER_RIGHT_19_PENALTY=0.073619
PRIMER_LEFT_19_SEQUENCE=TAACCGTTACCAAAAAGTCCTGA
PRIMER_RIGHT_19_SEQUENCE=TAACTGTTGCCCTCATAGCTCTC
PRIMER_LEFT_19=1061,23
PRIMER_RIGHT_19=1981,23
PRIMER_LEFT_19_TM=59.920
PRIMER_RIGHT_19_TM=59.926
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=921
=