PRIMER_SEQUENCE_ID=NM_005228
SEQUENCE=GCCGCGCTGCGCCGGAGTCCCGAGCTAGCCCCGGCGCCGCCGCCGCCCAGACCGGACGACAGGCCACCTCGTCGGCGTCCGCCCGAGTCCCCGCCTCGCCGCCAACGCCACAACCACCGCGCACGGCCCCCTGACTCCGTCCAGTATTGATCGGGAGAGCCGGAGCGAGCTCTTCGGGGAGCAGCGATGCGACCCTCCGGGACGGCCGGGGCAGCGCTCCTGGCGCTGCTGGCTGCGCTCTGCCCGGCGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAGCTCACGCAGTTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATGTTCAATAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTATGTGCAGAGGAATTATGATCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGTTATGTCCTCATTGCCCTCAACACAGTGGAGCGAATTCCTTTGGAAAACCTGCAGATCATCAGAGGAAATATGTACTACGAAAATTCCTATGCCTTAGCAGTCTTATCTAACTATGATGCAAATAAAACCGGACTGAAGGAGCTGCCCATGAGAAATTTACAGGAAATCCTGCATGGCGCCGTGCGGTTCAGCAACAACCCTGCCCTGTGCAACGTGGAGAGCATCCAGTGGCGGGACATAGTCAGCAGTGACTTTCTCAGCAACATGTCGATGGACTTCCAGAACCACCTGGGCAGCTGCCAAAAGTGTGATCCAAGCTGTCCCAATGGGAGCTGCTGGGGTGCAGGAGAGGAGAACTGCCAGAAACTGACCAAAATCATCTGTGCCCAGCAGTGCTCCGGGCGCTGCCGTGGCAAGTCCCCCAGTGACTGCTGCCACAACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAATTCCGAGACGAAGCCACGTGCAAGGACACCTGCCCCCCACTCATGCTCTACAACCCCACCACGTACCAGATGGATGTGAACCCCGAGGGCAAATACAGCTTTGGTGCCACCTGCGTGAAGAAGTGTCCCCGTAATTATGTGGTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGCTATGAGATGGAGGAAGACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACTCACTCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATCAGTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCCTTCACACATACTCCTCCTCTGGATCCACAGGAACTGGATATTCTGAAAACCGTAAAGGAAATCACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCTTTGAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTCTTGCAGTCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGATAAGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAATTATAAGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGCCATGCCTTGTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTGCGTCTCTTGCCGGAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAAGCTTCTGGAGGGTGAGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCAGAGTGCCTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATCCAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGTGTGCCACCTGTGCCATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGAAGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGGGGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGGAGGCTGCTGCAGGAGAGGGAGCTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACGGTGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAACTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAGTGCCTATCAAGTGGATGGCATTGGAATCAATTTTACACAGAATCTATACCCACCAGAGTGATGTCTGGAGCTACGGGGTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATATGTACCATCGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGACGCAGATAGTCGCCCAAAGTTCCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCTACCTTGTCATTCAGGGGGATGAAAGAATGCATTTGCCAAGTCCTACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGATGCCGACGAGTACCTCATCCCACAGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGCTCTCTGAGTGCAACCAGCAACAATTCCACCGTGGCTTGCATTGATAGAAATGGGCTGCAAAGCTGTCCCATCAAGGAAGACAGCTTCTTGCAGCGATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGACAGCATAGACGACACCTTCCTCCCAGTGCCTGAATACATAAACCAGTCCGTTCCCAAAAGGCCCGCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGCCTCTGAACCCCGCGCCCAGCAGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTGGGCAACCCCGAGTATCTCAACACTGTCCAGCCCACCTGTGTCAACAGCACATTCGACAGCCCTGCCCACTGGGCCCAGAAAGGCAGCCACCAAATTAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAAATGGCATCTTTAAGGGCTCCACAGCTGAAAATGCAGAATACCTAAGGGTCGCGCCACAAAGCAGTGAATTTATTGGAGCATGACCACGGAGGATAGTATGAGCCCTAAAAATCCAGACTCTTTCGATACCCAGGACCAAGCCACAGCAGGTCCTCCATCCCAACAGCCATGCCCGCATTAGCTCTTAGACCCACAGACTGGTTTTGCAACGTTTACACCGACTAGCCAGGAAGTACTTCCACCTCGGGCACATTTTGGGAAGTTGCATTCCTTTGTCTTCAAACTGTGAAGCATTTACAGAAACGCATCCAGCAAGAATATTGTCCCTTTGAGCAGAAATTTATCTTTCAAAGAGGTATATTTGAAAAAAAAAAAAAAAGTATATGTGAGGATTTTTATTGATTGGGGATCTTGGAGTTTTTCATTGTCGCTATTGATTTTTACTTCAATGGGCTCTTCCAACAAGGAAGAAGCTTGCTGGTAGCACTTGCTACCCTGAGTTCATCCAGGCCCAACTGTGAGCAAGGAGCACAAGCCACAAGTCTTCCAGAGGATGCTTGATTCCAGTGGTTCTGCTTCAAGGCTTCCACTGCAAAACACTAAAGATCCAAGAAGGCCTTCATGGCCCCAGCAGGCCGGATCGGTACTGTATCAAGTCATGGCAGGTACAGTAGGATAAGCCACTCTGTCCCTTCCTGGGCAAAGAAGAAACGGAGGGGATGAATTCTTCCTTAGACTTACTTTTGTAAAAATGTCCCCACGGTACTTACTCCCCACTGATGGACCAGTGGTTTCCAGTCATGAGCGTTAGACTGACTTGTTTGTCTTCCATTCCATTGTTTTGAAACTCAGTATGCCGCCCCTGTCTTGCTGTCATGAAATCAGCAAGAGAGGATGACACATCAAATAATAACTCGGATTCCAGCCCACATTGGATTCATCAGCATTTGGACCAATAGCCCACAGCTGAGAATGTGGAATACCTAAGGATAACACCGCTTTTGTTCTCGCAAAAACGTATCTCCTAATTTGAGGCTCAGATGAAATGCATCAGGTCCTTTGGGGCATAGATCAGAAGACTACAAAAATGAAGCTGCTCTGAAATCTCCTTTAGCCATCACCCCAACCCCCCAAAATTAGTTTGTGTTACTTATGGAAGATAGTTTTCTCCTTTTACTTCACTTCAAAAGCTTTTTACTCAAAGAGTATATGTTCCCTCCAGGTCAGCTGCCCCCAAACCCCCTCCTTACGCTTTGTCACACAAAAAGTGTCTCTGCCTTGAGTCATCTATTCAAGCACTTACAGCTCTGGCCACAACAGGGCATTTTACAGGTGCGAATGACAGTAGCATTATGAGTAGTGTGAATTCAGGTAGTAAATATGAAACTAGGGTTTGAAATTGATAATGCTTTCACAACATTTGCAGATGTTTTAGAAGGAAAAAAGTTCCTTCCTAAAATAATTTCTCTACAATTGGAAGATTGGAAGATTCAGCTAGTTAGGAGCCCATTTTTTCCTAATCTGTGTGTGCCCTGTAACCTGACTGGTTAACAGCAGTCCTTTGTAAACAGTGTTTTAAACTCTCCTAGTCAATATCCACCCCATCCAATTTATCAAGGAAGAAATGGTTCAGAAAATATTTTCAGCCTACAGTTATGTTCAGTCACACACACATACAAAATGTTCCTTTTGCTTTTAAAGTAATTTTTGACTCCCAGATCAGTCAGAGCCCCTACAGCATTGTTAAGAAAGTATTTGATTTTTGTCTCAATGAAAATAAAACTATATTCATTTCC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 64293, GC content failed 1620, low tm 23621, high tm 25440, high any compl 14, high end compl 52, high repeat similarity 52, long poly-x seq 118, ok 13376
PRIMER_RIGHT_EXPLAIN=considered 64302, GC content failed 1370, low tm 24138, high tm 25121, high any compl 1, high end compl 5, high repeat similarity 7, long poly-x seq 121, ok 13539
PRIMER_PAIR_EXPLAIN=considered 1915, unacceptable product size 1825, high any compl 1, high end compl 3, ok 86
PRIMER_PAIR_PENALTY=0.0148
PRIMER_LEFT_PENALTY=0.005234
PRIMER_RIGHT_PENALTY=0.009608
PRIMER_LEFT_SEQUENCE=ATATCCACCCCATCCAATTTATC
PRIMER_RIGHT_SEQUENCE=TGACTGATCTGGGAGTCAAAAAT
PRIMER_LEFT=5328,23
PRIMER_RIGHT=5461,23
PRIMER_LEFT_TM=60.005
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, LTR8 region
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.0239
PRIMER_LEFT_1_PENALTY=0.006308
PRIMER_RIGHT_1_PENALTY=0.017590
PRIMER_LEFT_1_SEQUENCE=GATCTTGGAGTTTTTCATTGTCG
PRIMER_RIGHT_1_SEQUENCE=GGCCTTCTTGGATCTTTAGTGTT
PRIMER_LEFT_1=4143,23
PRIMER_RIGHT_1=4353,23
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.0323
PRIMER_LEFT_2_PENALTY=0.015613
PRIMER_RIGHT_2_PENALTY=0.016658
PRIMER_LEFT_2_SEQUENCE=ATGAGCCCTAAAAATCCAGACTC
PRIMER_RIGHT_2_SEQUENCE=AATAGCGACAATGAAAAACTCCA
PRIMER_LEFT_2=3833,23
PRIMER_RIGHT_2=4170,23
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=60.017
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MER4C a consensus
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=338
PRIMER_PAIR_PENALTY_3=0.0323
PRIMER_LEFT_3_PENALTY=0.015613
PRIMER_RIGHT_3_PENALTY=0.016658
PRIMER_LEFT_3_SEQUENCE=ATGAGCCCTAAAAATCCAGACTC
PRIMER_RIGHT_3_SEQUENCE=CAATAGCGACAATGAAAAACTCC
PRIMER_LEFT_3=3833,23
PRIMER_RIGHT_3=4171,23
PRIMER_LEFT_3_TM=59.984
PRIMER_RIGHT_3_TM=60.017
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=339
PRIMER_PAIR_PENALTY_4=0.0342
PRIMER_LEFT_4_PENALTY=0.016658
PRIMER_RIGHT_4_PENALTY=0.017590
PRIMER_LEFT_4_SEQUENCE=TGGAGTTTTTCATTGTCGCTATT
PRIMER_RIGHT_4_SEQUENCE=GGCCTTCTTGGATCTTTAGTGTT
PRIMER_LEFT_4=4148,23
PRIMER_RIGHT_4=4353,23
PRIMER_LEFT_4_TM=60.017
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER2 Nonautonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=206
PRIMER_PAIR_PENALTY_5=0.0342
PRIMER_LEFT_5_PENALTY=0.016658
PRIMER_RIGHT_5_PENALTY=0.017590
PRIMER_LEFT_5_SEQUENCE=GGAGTTTTTCATTGTCGCTATTG
PRIMER_RIGHT_5_SEQUENCE=GGCCTTCTTGGATCTTTAGTGTT
PRIMER_LEFT_5=4149,23
PRIMER_RIGHT_5=4353,23
PRIMER_LEFT_5_TM=60.017
PRIMER_RIGHT_5_TM=60.018
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MER2 Nonautonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_5_END_STABILITY=6.2000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=205
PRIMER_PAIR_PENALTY_6=0.0348
PRIMER_LEFT_6_PENALTY=0.008837
PRIMER_RIGHT_6_PENALTY=0.025930
PRIMER_LEFT_6_SEQUENCE=TCACACATACTCCTCCTCTGGAT
PRIMER_RIGHT_6_SEQUENCE=GCACTCAGAGTTCTCCACAAACT
PRIMER_LEFT_6=1327,23
PRIMER_RIGHT_6=1850,23
PRIMER_LEFT_6_TM=60.009
PRIMER_RIGHT_6_TM=59.974
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=524
PRIMER_PAIR_PENALTY_7=0.0354
PRIMER_LEFT_7_PENALTY=0.009437
PRIMER_RIGHT_7_PENALTY=0.025930
PRIMER_LEFT_7_SEQUENCE=TCTGAAAACCGTAAAGGAAATCA
PRIMER_RIGHT_7_SEQUENCE=GCACTCAGAGTTCTCCACAAACT
PRIMER_LEFT_7=1367,23
PRIMER_RIGHT_7=1850,23
PRIMER_LEFT_7_TM=59.991
PRIMER_RIGHT_7_TM=59.974
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=484
PRIMER_PAIR_PENALTY_8=0.0417
PRIMER_LEFT_8_PENALTY=0.006308
PRIMER_RIGHT_8_PENALTY=0.035384
PRIMER_LEFT_8_SEQUENCE=GATCTTGGAGTTTTTCATTGTCG
PRIMER_RIGHT_8_SEQUENCE=AGGGACAGAGTGGCTTATCCTAC
PRIMER_LEFT_8=4143,23
PRIMER_RIGHT_8=4428,23
PRIMER_LEFT_8_TM=59.994
PRIMER_RIGHT_8_TM=60.035
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=52.174
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=6.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=286
PRIMER_PAIR_PENALTY_9=0.0503
PRIMER_LEFT_9_PENALTY=0.015613
PRIMER_RIGHT_9_PENALTY=0.034654
PRIMER_LEFT_9_SEQUENCE=ATGAGCCCTAAAAATCCAGACTC
PRIMER_RIGHT_9_SEQUENCE=GTTTGAAGACAAAGGAATGCAAC
PRIMER_LEFT_9=3833,23
PRIMER_RIGHT_9=4019,23
PRIMER_LEFT_9_TM=59.984
PRIMER_RIGHT_9_TM=60.035
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=187
PRIMER_PAIR_PENALTY_10=0.0510
PRIMER_LEFT_10_PENALTY=0.015613
PRIMER_RIGHT_10_PENALTY=0.035384
PRIMER_LEFT_10_SEQUENCE=ATGAGCCCTAAAAATCCAGACTC
PRIMER_RIGHT_10_SEQUENCE=AGGGACAGAGTGGCTTATCCTAC
PRIMER_LEFT_10=3833,23
PRIMER_RIGHT_10=4428,23
PRIMER_LEFT_10_TM=59.984
PRIMER_RIGHT_10_TM=60.035
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=52.174
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=596
PRIMER_PAIR_PENALTY_11=0.0513
PRIMER_LEFT_11_PENALTY=0.034654
PRIMER_RIGHT_11_PENALTY=0.016658
PRIMER_LEFT_11_SEQUENCE=GTTGCATTCCTTTGTCTTCAAAC
PRIMER_RIGHT_11_SEQUENCE=AATAGCGACAATGAAAAACTCCA
PRIMER_LEFT_11=3997,23
PRIMER_RIGHT_11=4170,23
PRIMER_LEFT_11_TM=60.035
PRIMER_RIGHT_11_TM=60.017
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=174
PRIMER_PAIR_PENALTY_12=0.0513
PRIMER_LEFT_12_PENALTY=0.034654
PRIMER_RIGHT_12_PENALTY=0.016658
PRIMER_LEFT_12_SEQUENCE=GTTGCATTCCTTTGTCTTCAAAC
PRIMER_RIGHT_12_SEQUENCE=CAATAGCGACAATGAAAAACTCC
PRIMER_LEFT_12=3997,23
PRIMER_RIGHT_12=4171,23
PRIMER_LEFT_12_TM=60.035
PRIMER_RIGHT_12_TM=60.017
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.0000
PRIMER_RIGHT_12_END_STABILITY=7.6000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=175
PRIMER_PAIR_PENALTY_13=0.0520
PRIMER_LEFT_13_PENALTY=0.016658
PRIMER_RIGHT_13_PENALTY=0.035384
PRIMER_LEFT_13_SEQUENCE=TGGAGTTTTTCATTGTCGCTATT
PRIMER_RIGHT_13_SEQUENCE=AGGGACAGAGTGGCTTATCCTAC
PRIMER_LEFT_13=4148,23
PRIMER_RIGHT_13=4428,23
PRIMER_LEFT_13_TM=60.017
PRIMER_RIGHT_13_TM=60.035
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=52.174
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER2 Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, MER4C a consensus
PRIMER_LEFT_13_END_STABILITY=5.9000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=281
PRIMER_PAIR_PENALTY_14=0.0520
PRIMER_LEFT_14_PENALTY=0.016658
PRIMER_RIGHT_14_PENALTY=0.035384
PRIMER_LEFT_14_SEQUENCE=GGAGTTTTTCATTGTCGCTATTG
PRIMER_RIGHT_14_SEQUENCE=AGGGACAGAGTGGCTTATCCTAC
PRIMER_LEFT_14=4149,23
PRIMER_RIGHT_14=4428,23
PRIMER_LEFT_14_TM=60.017
PRIMER_RIGHT_14_TM=60.035
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER2 Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=6.2000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=280
PRIMER_PAIR_PENALTY_15=0.0522
PRIMER_LEFT_15_PENALTY=0.034654
PRIMER_RIGHT_15_PENALTY=0.017590
PRIMER_LEFT_15_SEQUENCE=GTTGCATTCCTTTGTCTTCAAAC
PRIMER_RIGHT_15_SEQUENCE=GGCCTTCTTGGATCTTTAGTGTT
PRIMER_LEFT_15=3997,23
PRIMER_RIGHT_15=4353,23
PRIMER_LEFT_15_TM=60.035
PRIMER_RIGHT_15_TM=60.018
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=357
PRIMER_PAIR_PENALTY_16=0.0547
PRIMER_LEFT_16_PENALTY=0.006308
PRIMER_RIGHT_16_PENALTY=0.048411
PRIMER_LEFT_16_SEQUENCE=GATCTTGGAGTTTTTCATTGTCG
PRIMER_RIGHT_16_SEQUENCE=GGATCTTTAGTGTTTTGCAGTGG
PRIMER_LEFT_16=4143,23
PRIMER_RIGHT_16=4344,23
PRIMER_LEFT_16_TM=59.994
PRIMER_RIGHT_16_TM=60.048
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=202
PRIMER_PAIR_PENALTY_17=0.0566
PRIMER_LEFT_17_PENALTY=0.006308
PRIMER_RIGHT_17_PENALTY=0.050278
PRIMER_LEFT_17_SEQUENCE=GATCTTGGAGTTTTTCATTGTCG
PRIMER_RIGHT_17_SEQUENCE=AGTAAGTACCGTGGGGACATTTT
PRIMER_LEFT_17=4143,23
PRIMER_RIGHT_17=4506,23
PRIMER_LEFT_17_TM=59.994
PRIMER_RIGHT_17_TM=60.050
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=364
PRIMER_PAIR_PENALTY_18=0.0599
PRIMER_LEFT_18_PENALTY=0.050278
PRIMER_RIGHT_18_PENALTY=0.009608
PRIMER_LEFT_18_SEQUENCE=AAAATGTCCCCACGGTACTTACT
PRIMER_RIGHT_18_SEQUENCE=TGACTGATCTGGGAGTCAAAAAT
PRIMER_LEFT_18=4484,23
PRIMER_RIGHT_18=5461,23
PRIMER_LEFT_18_TM=60.050
PRIMER_RIGHT_18_TM=59.990
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MER4A a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.7000
PRIMER_RIGHT_18_END_STABILITY=7.2000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=978
PRIMER_PAIR_PENALTY_19=0.0640
PRIMER_LEFT_19_PENALTY=0.015613
PRIMER_RIGHT_19_PENALTY=0.048411
PRIMER_LEFT_19_SEQUENCE=ATGAGCCCTAAAAATCCAGACTC
PRIMER_RIGHT_19_SEQUENCE=GGATCTTTAGTGTTTTGCAGTGG
PRIMER_LEFT_19=3833,23
PRIMER_RIGHT_19=4344,23
PRIMER_LEFT_19_TM=59.984
PRIMER_RIGHT_19_TM=60.048
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=8.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=512
=