PRIMER_SEQUENCE_ID=NM_005232
SEQUENCE=GCCCCCGCCCGGCCCGCCCCGCTCTCCTAGTCCCTTGCAACCTGGCGCTGCATCCGGGCCACTGTCCCAGGTCCCAGGTCCCGGCCCGGAGCTATGGAGCGGCGCTGGCCCCTGGGGCTAGGGCTGGTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGGCGCGCGCCAAGGAAGTTACTCTGATGGACACAAGCAAGGCACAGGGAGAGCTGGGCTGGCTGCTGGATCCCCCAAAAGATGGGTGGAGTGAACAGCAACAGATACTGAATGGGACACCCCTCTACATGTACCAGGACTGCCCAATGCAAGGACGCAGAGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGGGAGGAGGCTTCCCGCGTCCACGTGGAGCTGCAGTTCACCGTGCGGGACTGCAAGAGTTTCCCTGGGGGAGCCGGGCCTCTGGGCTGCAAGGAGACCTTCAACCTTCTGTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGGCCCTTGTTCCAGAAGGTAACCACGGTGGCTGCAGACCAGAGCTTCACCATTCGAGACCTTGCGTCTGGCTCCGTGAAGCTGAATGTGGAGCGCTGCTCTCTGGGCCGCCTGACCCGCCGTGGCCTCTACCTCGCTTTCCACAACCCGGGTGCCTGTGTGGCCCTGGTGTCTGTCCGGGTCTTCTACCAGCGCTGTCCTGAGACCCTGAATGGCTTGGCCCAATTCCCAGACACTCTGCCTGGCCCCGCTGGGTTGGTGGAAGTGGCGGGCACCTGCTTGCCCCACGCGCGGGCCAGCCCCAGGCCCTCAGGTGCACCCCGCATGCACTGCAGCCCTGATGGCGAGTGGCTGGTGCCTGTAGGACGGTGCCACTGTGAGCCTGGCTATGAGGAAGGTGGCAGTGGCGAAGCATGTGTTGCCTGCCCTAGCGGCTCCTACCGGATGGACATGGACACACCCCATTGTCTCACGTGCCCCCAGCAGAGCACTGCTGAGTCTGAGGGGGCCACCATCTGTACCTGTGAGAGCGGCCATTACAGAGCTCCCGGGGAGGGCCCCCAGGTGGCATGCACAGGTCCCCCCTCGGCCCCCCGAAACCTGAGCTTCTCTGCCTCAGGGACTCAGCTCTCCCTGCGTTGGGAACCCCCAGCAGATACGGGGGGACGCCAGGATGTCAGATACAGTGTGAGGTGTTCCCAGTGTCAGGGCACAGCACAGGACGGGGGGCCCTGCCAGCCCTGTGGGGTGGGCGTGCACTTCTCGCCGGGGGCCCGGGCGCTCACCACACCTGCAGTGCATGTCAATGGCCTTGAACCTTATGCCAACTACACCTTTAATGTGGAAGCCCAAAATGGAGTGTCAGGGCTGGGCAGCTCTGGCCATGCCAGCACCTCAGTCAGCATCAGCATGGGGCATGCAGAGTCACTGTCAGGCCTGTCTCTGAGACTGGTGAAGAAAGAACCGAGGCAACTAGAGCTGACCTGGGCGGGGTCCCGGCCCCGAAGCCCTGGGGCGAACCTGACCTATGAGCTGCACGTGCTGAACCAGGATGAAGAACGGTACCAGATGGTTCTAGAACCCAGGGTCTTGCTGACAGAGCTGCAGCCTGACACCACATACATCGTCAGAGTCCGAATGCTGACCCCACTGGGTCCTGGCCCTTTCTCCCCTGATCATGAGTTTCGGACCAGCCCACCAGTGTCCAGGGGCCTGACTGGAGGAGAGATTGTAGCCGTCATCTTTGGGCTGCTGCTTGGTGCAGCCTTGCTGCTTGGGATTCTCGTTTTCCGGTCCAGGAGAGCCCAGCGGCAGAGGCAGCAGAGGCACGTGACCGCGCCACCGATGTGGATCGAGAGGACAAGCTGTGCTGAAGCCTTATGTGGTACCTCCAGGCATACGAGGACCCTGCACAGGGAGCCTTGGACTTTACCCGGAGGCTGGTCTAATTTTCCTTCCCGGGAGCTTGATCCAGCGTGGCTGATGGTGGACACTGTCATAGGAGAAGGAGAGTTTGGGGAAGTGTATCGAGGGACCCTCAGGCTCCCCAGCCAGGACTGCAAGACTGTGGCCATTAAGACCTTAAAAGACACATCCCCAGGTGGCCAGTGGTGGAACTTCCTTCGAGAGGCAACTATCATGGGCCAGTTTAGCCACCCGCATATTCTGCATCTGGAAGGCGTCGTCACAAAGCGAAAGCCGATCATGATCATCACAGAATTTATGGAGAATGCAGCCCTGGATGCCTTCCTGAGGGAGCGGGAGGACCAGCTGGTCCCTGGGCAGCTAGTGGCCATGCTGCAGGGCATAGCATCTGGCATGAACTACCTCAGTAATCACAATTATGTCCACCGGGACCTGGCTGCCAGAAACATCTTGGTGAATCAAAACCTGTGCTGCAAGGTGTCTGACTTTGGCCTGACTCGCCTCCTGGATGACTTTGATGGCACATACGAAACCCAGGGAGGAAAGATCCCTATCCGTTGGACAGCCCCTGAAGCCATTGCCCATCGGATCTTCACCACAGCCAGCGATGTGTGGAGCTTTGGGATTGTGATGTGGGAGGTGCTGAGCTTTGGGGACAAGCCTTATGGGGAGATGAGCAATCAGGAGGTTATGAAGAGCATTGAGGATGGGTACCGGTTGCCCCCTCCTGTGGACTGCCCTGCCCCTCTGTATGAGCTCATGAAGAACTGCTGGGCATATGACCGTGCCCGCCGGCCACACTTCCAGAAGCTTCAGGCACATCTGGAGCAACTGCTTGCCAACCCCCACTCCCTGCGGACCATTGCCAACTTTGACCCCAGGGTGACTCTTCGCCTGCCCAGCCTGAGTGGCTCAGATGGGATCCCGTATCGAACCGTCTCTGAGTGGCTCGAGTCCATACGCATGAAACGCTACATCCTGCACTTCCACTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACCGCTGAGGACCTGACGCAGATGGGAATCACACTGCCCGGGCACCAGAAGCGCATTCTTTGCAGTATTCAGGGATTCAAGGACTGATCCCTCCTCTCACCCCATGCCCAATCAGGGTGCAAGGAGCAAGGACGGGGCCAAGGTCGCTCATGGTCACTCCCTGCGCCCCTTCCCACAACCTGCCAGACTAGGCTATCGGTGCTGCTTCTGCCCGCTTTAAGGAGAACCCTGCTCTGCACCCCAGAAAACCTCTTTGTTTTAAAAGGGAGGTGGGGGTAGAAGTAAAAGGATGATCATGGGAGGGAGCTCAGGGGTTAATATATATACATACATACACATATATATATTGTTGTAAATAAACAGGAAATGATTTTCTGCCTCCATCCCACCCATCAGGGCTGCAGGCACT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 38765, GC content failed 1597, low tm 8949, high tm 20787, high any compl 3, high end compl 23, high repeat similarity 35, long poly-x seq 69, ok 7302
PRIMER_RIGHT_EXPLAIN=considered 38737, GC content failed 1685, low tm 9225, high tm 20400, high any compl 3, high end compl 7, high repeat similarity 18, long poly-x seq 71, ok 7328
PRIMER_PAIR_EXPLAIN=considered 1075, unacceptable product size 1003, high any compl 3, high end compl 2, ok 67
PRIMER_PAIR_PENALTY=0.1023
PRIMER_LEFT_PENALTY=0.043090
PRIMER_RIGHT_PENALTY=0.059210
PRIMER_LEFT_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_SEQUENCE=AGGTGTAGTTGGCATAAGGTTCA
PRIMER_LEFT=1175,23
PRIMER_RIGHT=1341,23
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=59.941
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MSTa consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1286
PRIMER_LEFT_1_PENALTY=0.085505
PRIMER_RIGHT_1_PENALTY=0.043090
PRIMER_LEFT_1_SEQUENCE=GACCTTCAACCTTCTGTACATGG
PRIMER_RIGHT_1_SEQUENCE=TCACACTGTATCTGACATCCTGG
PRIMER_LEFT_1=452,23
PRIMER_RIGHT_1=1197,23
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.043
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=746
PRIMER_PAIR_PENALTY_2=0.1447
PRIMER_LEFT_2_PENALTY=0.085505
PRIMER_RIGHT_2_PENALTY=0.059210
PRIMER_LEFT_2_SEQUENCE=GACCTTCAACCTTCTGTACATGG
PRIMER_RIGHT_2_SEQUENCE=AGGTGTAGTTGGCATAAGGTTCA
PRIMER_LEFT_2=452,23
PRIMER_RIGHT_2=1341,23
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.941
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MSTa consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=890
PRIMER_PAIR_PENALTY_3=0.1556
PRIMER_LEFT_3_PENALTY=0.043090
PRIMER_RIGHT_3_PENALTY=0.112479
PRIMER_LEFT_3_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_3_SEQUENCE=GATACACTTCCCCAAACTCTCCT
PRIMER_LEFT_3=1175,23
PRIMER_RIGHT_3=2034,23
PRIMER_LEFT_3_TM=60.043
PRIMER_RIGHT_3_TM=59.888
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=860
PRIMER_PAIR_PENALTY_4=0.1717
PRIMER_LEFT_4_PENALTY=0.059210
PRIMER_RIGHT_4_PENALTY=0.112479
PRIMER_LEFT_4_SEQUENCE=TGAACCTTATGCCAACTACACCT
PRIMER_RIGHT_4_SEQUENCE=GATACACTTCCCCAAACTCTCCT
PRIMER_LEFT_4=1319,23
PRIMER_RIGHT_4=2034,23
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=59.888
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=716
PRIMER_PAIR_PENALTY_5=0.2551
PRIMER_LEFT_5_PENALTY=0.142611
PRIMER_RIGHT_5_PENALTY=0.112479
PRIMER_LEFT_5_SEQUENCE=TTATGTGGTACCTCCAGGCATAC
PRIMER_RIGHT_5_SEQUENCE=GATACACTTCCCCAAACTCTCCT
PRIMER_LEFT_5=1884,23
PRIMER_RIGHT_5=2034,23
PRIMER_LEFT_5_TM=60.143
PRIMER_RIGHT_5_TM=59.888
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.6000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=151
PRIMER_PAIR_PENALTY_6=0.3255
PRIMER_LEFT_6_PENALTY=0.043090
PRIMER_RIGHT_6_PENALTY=0.282361
PRIMER_LEFT_6_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_6_SEQUENCE=GGTGTAGTTGGCATAAGGTTCAA
PRIMER_LEFT_6=1175,23
PRIMER_RIGHT_6=1340,23
PRIMER_LEFT_6_TM=60.043
PRIMER_RIGHT_6_TM=60.282
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=10.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=7.3000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=166
PRIMER_PAIR_PENALTY_7=0.3255
PRIMER_LEFT_7_PENALTY=0.043090
PRIMER_RIGHT_7_PENALTY=0.282361
PRIMER_LEFT_7_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_7_SEQUENCE=TCCACATTAAAGGTGTAGTTGGC
PRIMER_LEFT_7=1175,23
PRIMER_RIGHT_7=1351,23
PRIMER_LEFT_7_TM=60.043
PRIMER_RIGHT_7_TM=60.282
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=7.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=10.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=177
PRIMER_PAIR_PENALTY_8=0.3679
PRIMER_LEFT_8_PENALTY=0.085505
PRIMER_RIGHT_8_PENALTY=0.282361
PRIMER_LEFT_8_SEQUENCE=GACCTTCAACCTTCTGTACATGG
PRIMER_RIGHT_8_SEQUENCE=TCCACATTAAAGGTGTAGTTGGC
PRIMER_LEFT_8=452,23
PRIMER_RIGHT_8=1351,23
PRIMER_LEFT_8_TM=59.914
PRIMER_RIGHT_8_TM=60.282
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=10.0000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=900
PRIMER_PAIR_PENALTY_9=0.3948
PRIMER_LEFT_9_PENALTY=0.282361
PRIMER_RIGHT_9_PENALTY=0.112479
PRIMER_LEFT_9_SEQUENCE=TTGAACCTTATGCCAACTACACC
PRIMER_RIGHT_9_SEQUENCE=GATACACTTCCCCAAACTCTCCT
PRIMER_LEFT_9=1318,23
PRIMER_RIGHT_9=2034,23
PRIMER_LEFT_9_TM=60.282
PRIMER_RIGHT_9_TM=59.888
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MSTa consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=717
PRIMER_PAIR_PENALTY_10=0.4038
PRIMER_LEFT_10_PENALTY=0.142611
PRIMER_RIGHT_10_PENALTY=0.261219
PRIMER_LEFT_10_SEQUENCE=TTATGTGGTACCTCCAGGCATAC
PRIMER_RIGHT_10_SEQUENCE=TCTTCATAACCTCCTGATTGCTC
PRIMER_LEFT_10=1884,23
PRIMER_RIGHT_10=2628,23
PRIMER_LEFT_10_TM=60.143
PRIMER_RIGHT_10_TM=59.739
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.6000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=745
PRIMER_PAIR_PENALTY_11=0.4110
PRIMER_LEFT_11_PENALTY=0.142611
PRIMER_RIGHT_11_PENALTY=0.268402
PRIMER_LEFT_11_SEQUENCE=TTATGTGGTACCTCCAGGCATAC
PRIMER_RIGHT_11_SEQUENCE=CACAGGTTTTGATTCACCAAGAT
PRIMER_LEFT_11=1884,23
PRIMER_RIGHT_11=2401,23
PRIMER_LEFT_11_TM=60.143
PRIMER_RIGHT_11_TM=60.268
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.6000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=518
PRIMER_PAIR_PENALTY_12=0.4110
PRIMER_LEFT_12_PENALTY=0.142611
PRIMER_RIGHT_12_PENALTY=0.268402
PRIMER_LEFT_12_SEQUENCE=TTATGTGGTACCTCCAGGCATAC
PRIMER_RIGHT_12_SEQUENCE=ACAGGTTTTGATTCACCAAGATG
PRIMER_LEFT_12=1884,23
PRIMER_RIGHT_12=2400,23
PRIMER_LEFT_12_TM=60.143
PRIMER_RIGHT_12_TM=60.268
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=517
PRIMER_PAIR_PENALTY_13=0.4434
PRIMER_LEFT_13_PENALTY=0.043090
PRIMER_RIGHT_13_PENALTY=0.400326
PRIMER_LEFT_13_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_13_SEQUENCE=TGTAGTTGGCATAAGGTTCAAGG
PRIMER_LEFT_13=1175,23
PRIMER_RIGHT_13=1338,23
PRIMER_LEFT_13_TM=60.043
PRIMER_RIGHT_13_TM=60.400
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=10.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=164
PRIMER_PAIR_PENALTY_14=0.4472
PRIMER_LEFT_14_PENALTY=0.043090
PRIMER_RIGHT_14_PENALTY=0.404066
PRIMER_LEFT_14_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_14_SEQUENCE=AAGGTCTTAATGGCCACAGTCTT
PRIMER_LEFT_14=1175,23
PRIMER_RIGHT_14=2089,23
PRIMER_LEFT_14_TM=60.043
PRIMER_RIGHT_14_TM=60.404
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=915
PRIMER_PAIR_PENALTY_15=0.4587
PRIMER_LEFT_15_PENALTY=0.418512
PRIMER_RIGHT_15_PENALTY=0.040223
PRIMER_LEFT_15_SEQUENCE=AACAGCAACAGATACTGAATGGG
PRIMER_RIGHT_15_SEQUENCE=CTCCATGTACAGAAGGTTGAAGG
PRIMER_LEFT_15=250,23
PRIMER_RIGHT_15=476,23
PRIMER_LEFT_15_TM=60.419
PRIMER_RIGHT_15_TM=60.040
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, reverse MER21B a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.6000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=227
PRIMER_PAIR_PENALTY_16=0.4616
PRIMER_LEFT_16_PENALTY=0.418512
PRIMER_RIGHT_16_PENALTY=0.043090
PRIMER_LEFT_16_SEQUENCE=AACAGCAACAGATACTGAATGGG
PRIMER_RIGHT_16_SEQUENCE=TCACACTGTATCTGACATCCTGG
PRIMER_LEFT_16=250,23
PRIMER_RIGHT_16=1197,23
PRIMER_LEFT_16_TM=60.419
PRIMER_RIGHT_16_TM=60.043
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse MER21B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=948
PRIMER_PAIR_PENALTY_17=0.4633
PRIMER_LEFT_17_PENALTY=0.059210
PRIMER_RIGHT_17_PENALTY=0.404066
PRIMER_LEFT_17_SEQUENCE=TGAACCTTATGCCAACTACACCT
PRIMER_RIGHT_17_SEQUENCE=AAGGTCTTAATGGCCACAGTCTT
PRIMER_LEFT_17=1319,23
PRIMER_RIGHT_17=2089,23
PRIMER_LEFT_17_TM=59.941
PRIMER_RIGHT_17_TM=60.404
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MSTa consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MSTa consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=771
PRIMER_PAIR_PENALTY_18=0.4907
PRIMER_LEFT_18_PENALTY=0.043090
PRIMER_RIGHT_18_PENALTY=0.447629
PRIMER_LEFT_18_SEQUENCE=CCAGGATGTCAGATACAGTGTGA
PRIMER_RIGHT_18_SEQUENCE=TTAAGGTCTTAATGGCCACAGTC
PRIMER_LEFT_18=1175,23
PRIMER_RIGHT_18=2091,23
PRIMER_LEFT_18_TM=60.043
PRIMER_RIGHT_18_TM=59.552
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, THE1b consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=917
PRIMER_PAIR_PENALTY_19=0.4919
PRIMER_LEFT_19_PENALTY=0.230707
PRIMER_RIGHT_19_PENALTY=0.261219
PRIMER_LEFT_19_SEQUENCE=AAGACCTTAAAAGACACATCCCC
PRIMER_RIGHT_19_SEQUENCE=TCTTCATAACCTCCTGATTGCTC
PRIMER_LEFT_19=2082,23
PRIMER_RIGHT_19=2628,23
PRIMER_LEFT_19_TM=59.769
PRIMER_RIGHT_19_TM=59.739
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=10.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=547
=