PRIMER_SEQUENCE_ID=NM_005246
SEQUENCE=GTCACACCCTCGAATAATGACGCATACCTATCCTACTGTTACTGATCACCTAGTAATAATCTTGTAGTTCACATTACTCATTTTTCACCAAATTCTTTTGGTGAAGGACGCTTCAGAAACGGCCATCACTGAAGAGCAGACCCGTTTGGGTTCTCCACGCATTCTAGACTCCCGAAGAGCTCATGTTTTTTGGCTAGACCTATGACCATTTTCGCTAGACTTCACTGCACGTTTTCTCAAGTATCTTCTTTGTCCCTAATGTGTGACACCTCATCATGGACACGCTACTTTAGCTAAGGCATGACCAGCAATGAACAGTAGTAAGATATGTGCTGATTAGAAGGCTCACTTGTGCAGTGTGGAGGATAACCAGTGCCTTACAAAATGGGGTTTGGGAGTGACCTGAAGAATTCACATGAAGCAGTGTTAAAATTGCAAGACTGGGAATTACGGTTACTGGAAACAGTAAAGAAATTTATGGCCCTGAGAATAAAAAGTGATAAAGAATATGCATCTACTTTACAGAACCTTTGTAATCAAGTTGATAAGGAAAGTACTGTCCAAATGAATTATGTCAGCAACGTATCCAAGTCTTGGCTACTTATGATTCAGCAGACAGAACAACTTAGTAGGATAATGAAGACACATGCAGAGGACTTGAACTCTGGACCTTTACACAGGCTCACCATGATGATTAAGGACAAGCAGCAGGTGAAGAAAAGTTACATAGGTGTTCATCAGCAGATAGAGGCAGAGATGATCAAGGTTACCAAAACAGAATTGGAGAAGTTAAAATGCAGCTATAGACAATTAATAAAAGAAATGAATTCTGCCAAAGAGAAATATAAAGAAGCTTTAGCTAAAGGGAAGGAAACTGAAAAGGCCAAGGAACGATACGACAAAGCCACAATGAAACTTCATATGTTGCACAATCAGTATGTATTGGCGTTGAAAGGGGCACAGCTCCATCAGAATCAGTATTATGATATCACACTTCCCCTGCTTCTGGACTCCTTACAAAAGATGCAAGAAGAAATGATAAAAGCACTCAAAGGTATATTTGATGAATACAGCCAGATAACCAGTCTTGTCACAGAGGAAATAGTGAATGTCCATAAAGAGATTCAAATGTCGGTTGAACAGATAGATCCTAGTACAGAATACAATAATTTCATAGATGTTCACAGAACAACGGCTGCTAAAGAACAAGAAATAGAGTTTGATACTTCCTTACTGGAAGAAAATGAAAATCTTCAGGCAAATGAGATCATGTGGAATAACTTAACAGCAGAAAGTTTGCAAGTAATGTTGAAAACGTTAGCGGAAGAACTTATGCAAACACAGCAGATGCTTTTAAACAAGGAGGAGGCTGTTTTGGAGTTAGAGAAGAGAATTGAAGAATCTTCTGAAACTTGTGAGAAGAAGTCTGATATTGTGCTTCTGCTAAGCCAAAAACAGGCACTTGAAGAACTGAAACAGTCAGTCCAGCAGCTGAGATGCACTGAAGCAAAGTTTTCAGCACAGAAGGAATTACTAGAGCAAAAAGTGCAAGAAAATGATGGGAAAGAGCCACCTCCAGTAGTAAATTATGAAGAAGATGCACGATCAGTTACATCTATGGAAAGAAAGGAGAGGCTATCCAAATTTGAATCTATTCGTCATTCAATTGCTGGAATAATTAGGTCTCCAAAATCTGCAGTGGGCTCTTCAGCACTTTCTGATATGATCTCCATCAGTGAGAAGCCTTTGGCAGAACAGGACTGGTACCATGGTGCAATTCCCAGAATAGAAGCTCAAGAACTGTTAAAAAAACAAGGAGACTTTTTGGTGCGAGAGAGTCATGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGATGGACAGAGGAGACATTTTATCATACAATATGTTGATAACATGTATCGATTCGAGGGCACTGGGTTTTCAAACATTCCTCAACTTATAGATCATCACTATACAACAAAACAGGTCATCACTAAGAAATCAGGTGTAGTTCTGCTGAATCCTATTCCTAAGGACAAGAAATGGATTCTCAGTCATGAAGATGTCATATTGGGAGAATTACTGGGCAAGGGAAATTTTGGTGAAGTATATAAGGGCACATTAAAGGATAAAACTTCTGTTGCTGTTAAAACATGTAAAGAAGATCTTCCTCAGGAATTGAAAATAAAATTTTTACAAGAAGCCAAAATTCTCAAGCAATATGATCATCCCAATATTGTCAAACTTATAGGAGTTTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTTTCAGGAGGTGATTTCCTCACCTTTCTGAGAAGGAAGAAGGATGAACTAAAACTCAAACAGTTAGTGAAATTTTCATTAGACGCTGCTGCTGGTATGTTGTATCTCGAGAGTAAAAACTGTATACACAGGGACCTTGCTGCAAGAAACTGCCTGGTAGGTGAAAATAATGTTCTGAAAATCAGTGACTTTGGAATGTCTCGTCAAGAGGATGGTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCCATTAAATGGACAGCACCGGAAGCTCTTAATTATGGGAGATACAGTTCAGAGAGTGACGTGTGGAGCTTTGGCATCCTTCTCTGGGAGACCTTCAGCTTAGGGGTTTGTCCGTACCCTGGAATGACAAATCAGCAAGCAAGAGAGCAAGTAGAAAGAGGATACCGGATGTCAGCTCCCCAGCACTGTCCAGAGGATATTTCCAAAATCATGATGAAGTGTTGGGATTATAAACCTGAAAATCGCCCTAAGTTCAGTGAACTTCAGAAAGAGCTCACTATCATCAAGAGAAAACTCACATAGTGACAGGATGGCGCCAAACTCAGCCTTCAGGACTCTGTCCTCCAGCAGAGTAACATTATTGTTCTCATTAACAATGAATTTATACCACATTACCTTC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 33871, GC content failed 526, low tm 21079, high tm 4506, high any compl 6, high end compl 26, high repeat similarity 32, long poly-x seq 43, ok 7653
PRIMER_RIGHT_EXPLAIN=considered 33873, GC content failed 569, low tm 20820, high tm 4674, high end compl 5, high repeat similarity 2, long poly-x seq 43, ok 7760
PRIMER_PAIR_EXPLAIN=considered 458, unacceptable product size 405, high end compl 4, ok 49
PRIMER_PAIR_PENALTY=0.0247
PRIMER_LEFT_PENALTY=0.018344
PRIMER_RIGHT_PENALTY=0.006308
PRIMER_LEFT_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_SEQUENCE=TTCAACCGACATTTGAATCTCTT
PRIMER_LEFT=738,23
PRIMER_RIGHT=1139,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=402
PRIMER_PAIR_PENALTY_1=0.0294
PRIMER_LEFT_1_PENALTY=0.018344
PRIMER_RIGHT_1_PENALTY=0.011106
PRIMER_LEFT_1_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_1_SEQUENCE=CCTTTTCAGTTTCCTTCCCTTTA
PRIMER_LEFT_1=738,23
PRIMER_RIGHT_1=882,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.0364
PRIMER_LEFT_2_PENALTY=0.030114
PRIMER_RIGHT_2_PENALTY=0.006308
PRIMER_LEFT_2_SEQUENCE=TTCACTGCACGTTTTCTCAAGTA
PRIMER_RIGHT_2_SEQUENCE=TTCAACCGACATTTGAATCTCTT
PRIMER_LEFT_2=220,23
PRIMER_RIGHT_2=1139,23
PRIMER_LEFT_2_TM=59.970
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=920
PRIMER_PAIR_PENALTY_3=0.0388
PRIMER_LEFT_3_PENALTY=0.032476
PRIMER_RIGHT_3_PENALTY=0.006308
PRIMER_LEFT_3_SEQUENCE=ATTTTCGCTAGACTTCACTGCAC
PRIMER_RIGHT_3_SEQUENCE=TTCAACCGACATTTGAATCTCTT
PRIMER_LEFT_3=207,23
PRIMER_RIGHT_3=1139,23
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, L1MD1 3'-end of L1 repeat (subfamily L1MD1) - a consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=933
PRIMER_PAIR_PENALTY_4=0.0412
PRIMER_LEFT_4_PENALTY=0.030114
PRIMER_RIGHT_4_PENALTY=0.011106
PRIMER_LEFT_4_SEQUENCE=TTCACTGCACGTTTTCTCAAGTA
PRIMER_RIGHT_4_SEQUENCE=CCTTTTCAGTTTCCTTCCCTTTA
PRIMER_LEFT_4=220,23
PRIMER_RIGHT_4=882,23
PRIMER_LEFT_4_TM=59.970
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=663
PRIMER_PAIR_PENALTY_5=0.0415
PRIMER_LEFT_5_PENALTY=0.023182
PRIMER_RIGHT_5_PENALTY=0.018344
PRIMER_LEFT_5_SEQUENCE=TGCTGGAATAATTAGGTCTCCAA
PRIMER_RIGHT_5_SEQUENCE=GCCAGAAGATGAATACACTCCAC
PRIMER_LEFT_5=1667,23
PRIMER_RIGHT_5=2537,23
PRIMER_LEFT_5_TM=59.977
PRIMER_RIGHT_5_TM=60.018
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse L1PB1 3'-end of L1 repeat (subfamily L1PB1) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.5000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=871
PRIMER_PAIR_PENALTY_6=0.0415
PRIMER_LEFT_6_PENALTY=0.023182
PRIMER_RIGHT_6_PENALTY=0.018344
PRIMER_LEFT_6_SEQUENCE=TTGCTGGAATAATTAGGTCTCCA
PRIMER_RIGHT_6_SEQUENCE=GCCAGAAGATGAATACACTCCAC
PRIMER_LEFT_6=1666,23
PRIMER_RIGHT_6=2537,23
PRIMER_LEFT_6_TM=59.977
PRIMER_RIGHT_6_TM=60.018
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=872
PRIMER_PAIR_PENALTY_7=0.0415
PRIMER_LEFT_7_PENALTY=0.018344
PRIMER_RIGHT_7_PENALTY=0.023182
PRIMER_LEFT_7_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_7_SEQUENCE=TGGAGACCTAATTATTCCAGCAA
PRIMER_LEFT_7=738,23
PRIMER_RIGHT_7=1688,23
PRIMER_LEFT_7_TM=60.018
PRIMER_RIGHT_7_TM=59.977
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.5000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=951
PRIMER_PAIR_PENALTY_8=0.0415
PRIMER_LEFT_8_PENALTY=0.018344
PRIMER_RIGHT_8_PENALTY=0.023182
PRIMER_LEFT_8_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_8_SEQUENCE=TTGGAGACCTAATTATTCCAGCA
PRIMER_LEFT_8=738,23
PRIMER_RIGHT_8=1689,23
PRIMER_LEFT_8_TM=60.018
PRIMER_RIGHT_8_TM=59.977
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=6.5000
PRIMER_RIGHT_8_END_STABILITY=8.5000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=952
PRIMER_PAIR_PENALTY_9=0.0436
PRIMER_LEFT_9_PENALTY=0.032476
PRIMER_RIGHT_9_PENALTY=0.011106
PRIMER_LEFT_9_SEQUENCE=ATTTTCGCTAGACTTCACTGCAC
PRIMER_RIGHT_9_SEQUENCE=CCTTTTCAGTTTCCTTCCCTTTA
PRIMER_LEFT_9=207,23
PRIMER_RIGHT_9=882,23
PRIMER_LEFT_9_TM=59.968
PRIMER_RIGHT_9_TM=59.989
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=676
PRIMER_PAIR_PENALTY_10=0.0443
PRIMER_LEFT_10_PENALTY=0.018344
PRIMER_RIGHT_10_PENALTY=0.025930
PRIMER_LEFT_10_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_10_SEQUENCE=GCTGGACTGACTGTTTCAGTTCT
PRIMER_LEFT_10=738,23
PRIMER_RIGHT_10=1488,23
PRIMER_LEFT_10_TM=60.018
PRIMER_RIGHT_10_TM=59.974
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_10_END_STABILITY=6.5000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=751
PRIMER_PAIR_PENALTY_11=0.0453
PRIMER_LEFT_11_PENALTY=0.018344
PRIMER_RIGHT_11_PENALTY=0.026932
PRIMER_LEFT_11_SEQUENCE=GTGGAGTGTATTCATCTTCTGGC
PRIMER_RIGHT_11_SEQUENCE=TGACATCCGGTATCCTCTTTCTA
PRIMER_LEFT_11=2515,23
PRIMER_RIGHT_11=2723,23
PRIMER_LEFT_11_TM=60.018
PRIMER_RIGHT_11_TM=59.973
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=209
PRIMER_PAIR_PENALTY_12=0.0468
PRIMER_LEFT_12_PENALTY=0.018344
PRIMER_RIGHT_12_PENALTY=0.028502
PRIMER_LEFT_12_SEQUENCE=CAGCAGATAGAGGCAGAGATGAT
PRIMER_RIGHT_12_SEQUENCE=CTGTTTCAGTTCTTCAAGTGCCT
PRIMER_LEFT_12=738,23
PRIMER_RIGHT_12=1478,23
PRIMER_LEFT_12_TM=60.018
PRIMER_RIGHT_12_TM=59.971
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.5000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=741
PRIMER_PAIR_PENALTY_13=0.0485
PRIMER_LEFT_13_PENALTY=0.030114
PRIMER_RIGHT_13_PENALTY=0.018344
PRIMER_LEFT_13_SEQUENCE=TTCACTGCACGTTTTCTCAAGTA
PRIMER_RIGHT_13_SEQUENCE=ATCATCTCTGCCTCTATCTGCTG
PRIMER_LEFT_13=220,23
PRIMER_RIGHT_13=760,23
PRIMER_LEFT_13_TM=59.970
PRIMER_RIGHT_13_TM=60.018
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, GAGACA Primate simple repeat pattern
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_13_END_STABILITY=5.7000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=541
PRIMER_PAIR_PENALTY_14=0.0491
PRIMER_LEFT_14_PENALTY=0.025930
PRIMER_RIGHT_14_PENALTY=0.023182
PRIMER_LEFT_14_SEQUENCE=AGAACTGAAACAGTCAGTCCAGC
PRIMER_RIGHT_14_SEQUENCE=TGGAGACCTAATTATTCCAGCAA
PRIMER_LEFT_14=1466,23
PRIMER_RIGHT_14=1688,23
PRIMER_LEFT_14_TM=59.974
PRIMER_RIGHT_14_TM=59.977
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=9.7000
PRIMER_RIGHT_14_END_STABILITY=8.5000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=223
PRIMER_PAIR_PENALTY_15=0.0491
PRIMER_LEFT_15_PENALTY=0.025930
PRIMER_RIGHT_15_PENALTY=0.023182
PRIMER_LEFT_15_SEQUENCE=AGAACTGAAACAGTCAGTCCAGC
PRIMER_RIGHT_15_SEQUENCE=TTGGAGACCTAATTATTCCAGCA
PRIMER_LEFT_15=1466,23
PRIMER_RIGHT_15=1689,23
PRIMER_LEFT_15_TM=59.974
PRIMER_RIGHT_15_TM=59.977
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, MER34 a consensus
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=8.5000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=224
PRIMER_PAIR_PENALTY_16=0.0503
PRIMER_LEFT_16_PENALTY=0.031944
PRIMER_RIGHT_16_PENALTY=0.018344
PRIMER_LEFT_16_SEQUENCE=TGCACGATCAGTTACATCTATGG
PRIMER_RIGHT_16_SEQUENCE=GCCAGAAGATGAATACACTCCAC
PRIMER_LEFT_16=1598,23
PRIMER_RIGHT_16=2537,23
PRIMER_LEFT_16_TM=60.032
PRIMER_RIGHT_16_TM=60.018
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.4000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=940
PRIMER_PAIR_PENALTY_17=0.0508
PRIMER_LEFT_17_PENALTY=0.032476
PRIMER_RIGHT_17_PENALTY=0.018344
PRIMER_LEFT_17_SEQUENCE=ATTTTCGCTAGACTTCACTGCAC
PRIMER_RIGHT_17_SEQUENCE=ATCATCTCTGCCTCTATCTGCTG
PRIMER_LEFT_17=207,23
PRIMER_RIGHT_17=760,23
PRIMER_LEFT_17_TM=59.968
PRIMER_RIGHT_17_TM=60.018
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, GAGACA Primate simple repeat pattern
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=8.5000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=554
PRIMER_PAIR_PENALTY_18=0.0517
PRIMER_LEFT_18_PENALTY=0.028502
PRIMER_RIGHT_18_PENALTY=0.023182
PRIMER_LEFT_18_SEQUENCE=AGGCACTTGAAGAACTGAAACAG
PRIMER_RIGHT_18_SEQUENCE=TTGGAGACCTAATTATTCCAGCA
PRIMER_LEFT_18=1456,23
PRIMER_RIGHT_18=1689,23
PRIMER_LEFT_18_TM=59.971
PRIMER_RIGHT_18_TM=59.977
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=8.5000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=234
PRIMER_PAIR_PENALTY_19=0.0517
PRIMER_LEFT_19_PENALTY=0.028502
PRIMER_RIGHT_19_PENALTY=0.023182
PRIMER_LEFT_19_SEQUENCE=AGGCACTTGAAGAACTGAAACAG
PRIMER_RIGHT_19_SEQUENCE=TGGAGACCTAATTATTCCAGCAA
PRIMER_LEFT_19=1456,23
PRIMER_RIGHT_19=1688,23
PRIMER_LEFT_19_TM=59.971
PRIMER_RIGHT_19_TM=59.977
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=233
=