PRIMER_SEQUENCE_ID=NM_005248
SEQUENCE=GGGAGGACCCCAATCTAGGCCCAAGAGGGAAAGGCCACGTGCCTGTATGAGCGTATGAGCATGTGCATGCGCGTGTGTGCACAGGGTGGTGCACCTGGCAGGGGTCCTTGAGTGAGGCATGCCCCATTCTGTAGCAGGGAACCTGGAATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGCCTGGAAGGGGACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAGGCCCGGCCTGCATCCTCATTTGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCCATCAACCCTGGCTTCCTTGATAGTGGCACCATCAGGGGTGTGTCAGGGATTGGGGTGACCCTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGGATGACCTCACCTTCACCAAGGGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGCTCGGTCTCTCAGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAAGCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCACCAGGCAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTACTCCCTGTCCATCCGGGACTGGGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATCCGCAAACTGGACATGGGCGGCTACTACATCACCACACGGGTTCAGTTCAACTCGGTGCAGGAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGTGCAACCTGCTCATCGCGCCCTGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGAGATCAGCCGCAGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGCACGTGGAACGGCAGCACTAAGGTGGCGGTGAAGACGCTGAAGCCGGGCACCATGTCCCCGAAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAGCTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGCAGCTTGCTGGATTTTCTCAAGAACCCAGAGGGCCAGGATTTGAGGCTGCCCCAATTGGTGGACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGGAACGCATGAACTACATTCACCGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAAGATCGCAGACTTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTCCCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGACGTGTGGTCCTTTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCAGGCATGAATAAACGGGAAGTGTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCTCCAGGCTGCCCAGCATCCCTGTACGAGGCCATGGAACAGACCTGGCGTCTGGACCCGGAGGAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGGACTACTTCACCTCCGCTGAACCACAGTACCAGCCCGGGGATCAGACATAGCCTGTCCGGGCATCAACCCTCTCTGGCGGTGGCCACCAGTCCTTGCCAATCCCCAGAGCTGTTCTTCCAAAGCCCCCAGGCTGGCTTAGAACCCCATAGAGTCCTAGCATCACCGAGGACGTGGCTGCTCTGACACCACCTAGGGCAACCTACTTGTTTTACAGATGGGGCAAAAGGAGGCCCAGAGCTGATCTCTCATCCGCTCTGGCCCCAAGCACTATTTCTTCCTTTTCCACTTAGGCCCCTACATGCCTGTAGCCCTTTCTCACTCCATCCCCACCCAAAAGTGCTCAGACCTTGTCTAGTTATTTATAAACTGTATGTACCTCCCTCACTTCTCTCCTATCACTGCTTTCCTACTCTCCTTTTATCTCACTCTAGTCCAGGTGCCAAGAATTTCCCTTCTACCCTCTATTCTCTTGTGTCTGTAAGTTACAAAGTCAGGAAAAGTCTTGGCTGGACCCCTTTCCTGCTGGGTGGATGCAGTGGTCCAGGACTGGGGTCTGGGCCCAGGTTTGAGGGAGAAGTTTGCAGAGCACTTCCCACCTCTCTGAATAGTGTGTATGTGTTGATTTATTGATTCTGTAAATAAGTAAAATGACAATATGAATCCTCCA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27060, GC content failed 214, low tm 7129, high tm 14331, high any compl 3, high end compl 17, high repeat similarity 26, ok 5340
PRIMER_RIGHT_EXPLAIN=considered 27060, GC content failed 376, low tm 7547, high tm 13845, high any compl 1, high end compl 9, high repeat similarity 6, ok 5276
PRIMER_PAIR_EXPLAIN=considered 258, unacceptable product size 206, high end compl 11, ok 41
PRIMER_PAIR_PENALTY=0.1467
PRIMER_LEFT_PENALTY=0.094274
PRIMER_RIGHT_PENALTY=0.052460
PRIMER_LEFT_SEQUENCE=ATCTACATCGTGACCGAGTTCAT
PRIMER_RIGHT_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT=1134,23
PRIMER_RIGHT=1468,23
PRIMER_LEFT_TM=59.906
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=335
PRIMER_PAIR_PENALTY_1=0.2156
PRIMER_LEFT_1_PENALTY=0.170187
PRIMER_RIGHT_1_PENALTY=0.045432
PRIMER_LEFT_1_SEQUENCE=CTTCCTGGAGGACTACTTCACCT
PRIMER_RIGHT_1_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_1=1676,23
PRIMER_RIGHT_1=2299,23
PRIMER_LEFT_1_TM=60.170
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=624
PRIMER_PAIR_PENALTY_2=0.2966
PRIMER_LEFT_2_PENALTY=0.251196
PRIMER_RIGHT_2_PENALTY=0.045432
PRIMER_LEFT_2_SEQUENCE=TCTCATCAAGGACGATGAGTACA
PRIMER_RIGHT_2_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_2=1361,23
PRIMER_RIGHT_2=2299,23
PRIMER_LEFT_2_TM=59.749
PRIMER_RIGHT_2_TM=60.045
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=939
PRIMER_PAIR_PENALTY_3=0.3037
PRIMER_LEFT_3_PENALTY=0.251196
PRIMER_RIGHT_3_PENALTY=0.052460
PRIMER_LEFT_3_SEQUENCE=TCTCATCAAGGACGATGAGTACA
PRIMER_RIGHT_3_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_3=1361,23
PRIMER_RIGHT_3=1468,23
PRIMER_LEFT_3_TM=59.749
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.3074
PRIMER_LEFT_4_PENALTY=0.094274
PRIMER_RIGHT_4_PENALTY=0.213146
PRIMER_LEFT_4_SEQUENCE=ATCTACATCGTGACCGAGTTCAT
PRIMER_RIGHT_4_SEQUENCE=GGCACCTGGACTAGAGTGAGATA
PRIMER_LEFT_4=1134,23
PRIMER_RIGHT_4=2127,23
PRIMER_LEFT_4_TM=59.906
PRIMER_RIGHT_4_TM=59.787
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.174
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=994
PRIMER_PAIR_PENALTY_5=0.3860
PRIMER_LEFT_5_PENALTY=0.333513
PRIMER_RIGHT_5_PENALTY=0.052460
PRIMER_LEFT_5_SEQUENCE=CAAGCTGAAGAGTGGTACTTTGG
PRIMER_RIGHT_5_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_5=564,23
PRIMER_RIGHT_5=1468,23
PRIMER_LEFT_5_TM=60.334
PRIMER_RIGHT_5_TM=60.052
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.8000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=905
PRIMER_PAIR_PENALTY_6=0.4559
PRIMER_LEFT_6_PENALTY=0.410501
PRIMER_RIGHT_6_PENALTY=0.045432
PRIMER_LEFT_6_SEQUENCE=GGGCAACCTACTTGTTTTACAGA
PRIMER_RIGHT_6_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_6=1879,23
PRIMER_RIGHT_6=2299,23
PRIMER_LEFT_6_TM=59.589
PRIMER_RIGHT_6_TM=60.045
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=421
PRIMER_PAIR_PENALTY_7=0.4643
PRIMER_LEFT_7_PENALTY=0.251196
PRIMER_RIGHT_7_PENALTY=0.213146
PRIMER_LEFT_7_SEQUENCE=TCTCATCAAGGACGATGAGTACA
PRIMER_RIGHT_7_SEQUENCE=GGCACCTGGACTAGAGTGAGATA
PRIMER_LEFT_7=1361,23
PRIMER_RIGHT_7=2127,23
PRIMER_LEFT_7_TM=59.749
PRIMER_RIGHT_7_TM=59.787
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.4000
PRIMER_RIGHT_7_END_STABILITY=5.6000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=767
PRIMER_PAIR_PENALTY_8=0.4821
PRIMER_LEFT_8_PENALTY=0.429676
PRIMER_RIGHT_8_PENALTY=0.052460
PRIMER_LEFT_8_SEQUENCE=GACTCAATCCAAGCTGAAGAGTG
PRIMER_RIGHT_8_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_8=555,23
PRIMER_RIGHT_8=1468,23
PRIMER_LEFT_8_TM=60.430
PRIMER_RIGHT_8_TM=60.052
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=914
PRIMER_PAIR_PENALTY_9=0.4821
PRIMER_LEFT_9_PENALTY=0.429676
PRIMER_RIGHT_9_PENALTY=0.052460
PRIMER_LEFT_9_SEQUENCE=TGACTCAATCCAAGCTGAAGAGT
PRIMER_RIGHT_9_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_9=554,23
PRIMER_RIGHT_9=1468,23
PRIMER_LEFT_9_TM=60.430
PRIMER_RIGHT_9_TM=60.052
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.1000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=915
PRIMER_PAIR_PENALTY_10=0.5025
PRIMER_LEFT_10_PENALTY=0.457043
PRIMER_RIGHT_10_PENALTY=0.045432
PRIMER_LEFT_10_SEQUENCE=AGAACCCCATAGAGTCCTAGCAT
PRIMER_RIGHT_10_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_10=1824,23
PRIMER_RIGHT_10=2299,23
PRIMER_LEFT_10_TM=59.543
PRIMER_RIGHT_10_TM=60.045
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=476
PRIMER_PAIR_PENALTY_11=0.5048
PRIMER_LEFT_11_PENALTY=0.094274
PRIMER_RIGHT_11_PENALTY=0.410501
PRIMER_LEFT_11_SEQUENCE=ATCTACATCGTGACCGAGTTCAT
PRIMER_RIGHT_11_SEQUENCE=TCTGTAAAACAAGTAGGTTGCCC
PRIMER_LEFT_11=1134,23
PRIMER_RIGHT_11=1901,23
PRIMER_LEFT_11_TM=59.906
PRIMER_RIGHT_11_TM=59.589
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.9000
PRIMER_RIGHT_11_END_STABILITY=11.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=768
PRIMER_PAIR_PENALTY_12=0.5352
PRIMER_LEFT_12_PENALTY=0.489788
PRIMER_RIGHT_12_PENALTY=0.045432
PRIMER_LEFT_12_SEQUENCE=CTGGCTTAGAACCCCATAGAGTC
PRIMER_RIGHT_12_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_12=1817,23
PRIMER_RIGHT_12=2299,23
PRIMER_LEFT_12_TM=60.490
PRIMER_RIGHT_12_TM=60.045
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.1000
PRIMER_RIGHT_12_END_STABILITY=9.7000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=483
PRIMER_PAIR_PENALTY_13=0.5361
PRIMER_LEFT_13_PENALTY=0.490704
PRIMER_RIGHT_13_PENALTY=0.045432
PRIMER_LEFT_13_SEQUENCE=ACCTAGGGCAACCTACTTGTTTT
PRIMER_RIGHT_13_SEQUENCE=ACACACTATTCAGAGAGGTGGGA
PRIMER_LEFT_13=1874,23
PRIMER_RIGHT_13=2299,23
PRIMER_LEFT_13_TM=59.509
PRIMER_RIGHT_13_TM=60.045
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=426
PRIMER_PAIR_PENALTY_14=0.5560
PRIMER_LEFT_14_PENALTY=0.094274
PRIMER_RIGHT_14_PENALTY=0.461726
PRIMER_LEFT_14_SEQUENCE=ATCTACATCGTGACCGAGTTCAT
PRIMER_RIGHT_14_SEQUENCE=GCAGTGATAGGAGAGAAGTGAGG
PRIMER_LEFT_14=1134,23
PRIMER_RIGHT_14=2088,23
PRIMER_LEFT_14_TM=59.906
PRIMER_RIGHT_14_TM=59.538
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=955
PRIMER_PAIR_PENALTY_15=0.5807
PRIMER_LEFT_15_PENALTY=0.170187
PRIMER_RIGHT_15_PENALTY=0.410501
PRIMER_LEFT_15_SEQUENCE=CTTCCTGGAGGACTACTTCACCT
PRIMER_RIGHT_15_SEQUENCE=TCTGTAAAACAAGTAGGTTGCCC
PRIMER_LEFT_15=1676,23
PRIMER_RIGHT_15=1901,23
PRIMER_LEFT_15_TM=60.170
PRIMER_RIGHT_15_TM=59.589
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=11.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=226
PRIMER_PAIR_PENALTY_16=0.5841
PRIMER_LEFT_16_PENALTY=0.094274
PRIMER_RIGHT_16_PENALTY=0.489788
PRIMER_LEFT_16_SEQUENCE=ATCTACATCGTGACCGAGTTCAT
PRIMER_RIGHT_16_SEQUENCE=GACTCTATGGGGTTCTAAGCCAG
PRIMER_LEFT_16=1134,23
PRIMER_RIGHT_16=1839,23
PRIMER_LEFT_16_TM=59.906
PRIMER_RIGHT_16_TM=60.490
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=706
PRIMER_PAIR_PENALTY_17=0.5888
PRIMER_LEFT_17_PENALTY=0.536322
PRIMER_RIGHT_17_PENALTY=0.052460
PRIMER_LEFT_17_SEQUENCE=AAGCTGAAGAGTGGTACTTTGGA
PRIMER_RIGHT_17_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_17=565,23
PRIMER_RIGHT_17=1468,23
PRIMER_LEFT_17_TM=59.464
PRIMER_RIGHT_17_TM=60.052
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=904
PRIMER_PAIR_PENALTY_18=0.5888
PRIMER_LEFT_18_PENALTY=0.536322
PRIMER_RIGHT_18_PENALTY=0.052460
PRIMER_LEFT_18_SEQUENCE=AGCTGAAGAGTGGTACTTTGGAA
PRIMER_RIGHT_18_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_18=566,23
PRIMER_RIGHT_18=1468,23
PRIMER_LEFT_18_TM=59.464
PRIMER_RIGHT_18_TM=60.052
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.5000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=903
PRIMER_PAIR_PENALTY_19=0.5888
PRIMER_LEFT_19_PENALTY=0.536322
PRIMER_RIGHT_19_PENALTY=0.052460
PRIMER_LEFT_19_SEQUENCE=TCCAAGCTGAAGAGTGGTACTTT
PRIMER_RIGHT_19_SEQUENCE=ACGTCTGACTTGATGGTGAATCT
PRIMER_LEFT_19=562,23
PRIMER_RIGHT_19=1468,23
PRIMER_LEFT_19_TM=59.464
PRIMER_RIGHT_19_TM=60.052
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=907
=