PRIMER_SEQUENCE_ID=NM_005356
SEQUENCE=CGCCTGGACCATGTGAATGGGGCCAGAGGGCTCCCGGGCTGGGCAGGGACCATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATGTGTGTGAGAACTGCCATTATCCCATAGTCCCACTGGATGGCAAGGGCACGCTGCTCATCCGAAATGGCTCTGAGGTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCCGCCGGCTTCCCCACTGCAAGACAACCTGGTTATCGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTGAGAAGGGGGAACCACTCCGCATCCTGGAGCAGAGCGGCGAGTGGTGGAAGGCGCAGTCCCTGACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGTGGCCAAAGCGAACAGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACGCGGAGCGGCAGCTCCTGGCGCCCGGGAACACTCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCCGGGTCCTTTTCACTGTCGGTCCGGGACTTCGACCAAAACCAGGGAGAGGTGGTGAAACATTACAAGATCCGTAATCTGGACAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTTCCCGGCCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCTGTGCACACGGTTGAGCCGCCCCTGCCAGACCCAGAAGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTTCCCAGGGAGACGCTGAAGCTGGTGGAGCGGCTGGGGGCTGGACAGTTCGGGGAGGTGTGGATGGGGTACTACAACGGGCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATGTCCCCGGACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTCTACGCTGTGGTCACCCAGGAGCCCATCTACATCATCACTGAATACATGGAGAATGGGAGTCTAGTGGATTTTCTCAAGACCCCTTCAGGCATCAAGTTGACCATCAACAAACTCCTGGACATGGCAGCCCAAATTGCAGAAGGCATGGCATTCATTGAAGAGCGGAATTATATTCATCGTGACCTTCGGGCTGCCAACATTCTGGTGTCTGACACCCTGAGCTGCAAGATTGCAGACTTTGGCCTAGCACGCCTCATTGAGGACAACGAGTACACAGCCAGGGAGGGGGCCAAGTTTCCCATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATCAAGTCAGATGTGTGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCACGGCCGCATCCCTTACCCAGGGATGACCAACCCGGAGGTGATTCAGAACCTGGAGCGAGGCTACCGCATGGTGCGCCCTGACAACTGTCCAGAGGAGCTGTACCAACTCATGAGGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCACCTTTGACTACCTGCGCAGTGTGCTGGAGGACTTCTTCACGGCCACAGAGGGCCAGTACCAGCCTCAGCCTTGAGAGGAGGCCTTGAGAGGCCCTGGGGTTCTCCCCCTTTCTCTCCAGCCTGACTTGGGGAGATGGAGTTCTTGTGCCATAGTCACATGGCCTATGCACATATGGACTCTGCACATGAATCCCACCCACATGTGACACATATGCACCTTGTGTCTGTACACGTGTCCTGTAGTTGCGTGGACTCTGCACATGTCTTGTGCATGTGTAGCCTGTGCATGTATGTCTTGGACACTGTACAAGGTACCCCTTTCTGGCTCTCCCATTTCCTGAGACCACCAGAGAGAGGGGAGAAGCCTGGGATTGACAGAAGCTTCTGCCCACCTACTTTTCTTTCCTCAGATCATCCAGAAGTTCCTCAAGGGCCAGGACTTTATCTAATACCTCTGTGTGCTCCTCCTTGGTGCCTGGCCTGGCACACATCAGGAGTTCAATAAATGTCTGTTGATGACTGCCG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 23196, GC content failed 237, low tm 6060, high tm 12200, high any compl 3, high end compl 26, high repeat similarity 46, ok 4624
PRIMER_RIGHT_EXPLAIN=considered 23196, GC content failed 132, low tm 6431, high tm 11875, high any compl 1, high end compl 3, high repeat similarity 1, ok 4753
PRIMER_PAIR_EXPLAIN=considered 873, unacceptable product size 812, high end compl 9, ok 52
PRIMER_PAIR_PENALTY=0.1408
PRIMER_LEFT_PENALTY=0.122456
PRIMER_RIGHT_PENALTY=0.018344
PRIMER_LEFT_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_SEQUENCE=GACTATGGCACAAGAACTCCATC
PRIMER_LEFT=1285,23
PRIMER_RIGHT=1661,23
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=377
PRIMER_PAIR_PENALTY_1=0.2338
PRIMER_LEFT_1_PENALTY=0.147950
PRIMER_RIGHT_1_PENALTY=0.085878
PRIMER_LEFT_1_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_1_SEQUENCE=CTGTCCACTTAATGGGAAACTTG
PRIMER_LEFT_1=1007,23
PRIMER_RIGHT_1=1272,23
PRIMER_LEFT_1_TM=59.852
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.2419
PRIMER_LEFT_2_PENALTY=0.122456
PRIMER_RIGHT_2_PENALTY=0.119476
PRIMER_LEFT_2_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_2_SEQUENCE=ATTAGATAAAGTCCTGGCCCTTG
PRIMER_LEFT_2=1285,23
PRIMER_RIGHT_2=1956,23
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=59.881
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=672
PRIMER_PAIR_PENALTY_3=0.2477
PRIMER_LEFT_3_PENALTY=0.229390
PRIMER_RIGHT_3_PENALTY=0.018344
PRIMER_LEFT_3_SEQUENCE=GCCCATCTACATCATCACTGAAT
PRIMER_RIGHT_3_SEQUENCE=GACTATGGCACAAGAACTCCATC
PRIMER_LEFT_3=980,23
PRIMER_RIGHT_3=1661,23
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=682
PRIMER_PAIR_PENALTY_4=0.2661
PRIMER_LEFT_4_PENALTY=0.247806
PRIMER_RIGHT_4_PENALTY=0.018344
PRIMER_LEFT_4_SEQUENCE=GGACATTCACCATCAAGTCAGAT
PRIMER_RIGHT_4_SEQUENCE=GACTATGGCACAAGAACTCCATC
PRIMER_LEFT_4=1294,23
PRIMER_RIGHT_4=1661,23
PRIMER_LEFT_4_TM=60.248
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, reverse MER31 a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=368
PRIMER_PAIR_PENALTY_5=0.2668
PRIMER_LEFT_5_PENALTY=0.122456
PRIMER_RIGHT_5_PENALTY=0.144318
PRIMER_LEFT_5_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_5_SEQUENCE=CTATGGCACAAGAACTCCATCTC
PRIMER_LEFT_5=1285,23
PRIMER_RIGHT_5=1659,23
PRIMER_LEFT_5_TM=60.122
PRIMER_RIGHT_5_TM=60.144
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.3000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=375
PRIMER_PAIR_PENALTY_6=0.2674
PRIMER_LEFT_6_PENALTY=0.147950
PRIMER_RIGHT_6_PENALTY=0.119476
PRIMER_LEFT_6_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_6_SEQUENCE=ATTAGATAAAGTCCTGGCCCTTG
PRIMER_LEFT_6=1007,23
PRIMER_RIGHT_6=1956,23
PRIMER_LEFT_6_TM=59.852
PRIMER_RIGHT_6_TM=59.881
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=8.1000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=950
PRIMER_PAIR_PENALTY_7=0.2807
PRIMER_LEFT_7_PENALTY=0.262326
PRIMER_RIGHT_7_PENALTY=0.018344
PRIMER_LEFT_7_SEQUENCE=AGAAGCCATTAACTACGGGACAT
PRIMER_RIGHT_7_SEQUENCE=GACTATGGCACAAGAACTCCATC
PRIMER_LEFT_7=1277,23
PRIMER_RIGHT_7=1661,23
PRIMER_LEFT_7_TM=60.262
PRIMER_RIGHT_7_TM=60.018
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=385
PRIMER_PAIR_PENALTY_8=0.2878
PRIMER_LEFT_8_PENALTY=0.122456
PRIMER_RIGHT_8_PENALTY=0.165371
PRIMER_LEFT_8_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_8_SEQUENCE=AAAGAAAAGTAGGTGGGCAGAAG
PRIMER_LEFT_8=1285,23
PRIMER_RIGHT_8=1910,23
PRIMER_LEFT_8_TM=60.122
PRIMER_RIGHT_8_TM=60.165
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=626
PRIMER_PAIR_PENALTY_9=0.2879
PRIMER_LEFT_9_PENALTY=0.122456
PRIMER_RIGHT_9_PENALTY=0.165486
PRIMER_LEFT_9_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_9_SEQUENCE=CTTCTGGATGATCTGAGGAAAGA
PRIMER_LEFT_9=1285,23
PRIMER_RIGHT_9=1928,23
PRIMER_LEFT_9_TM=60.122
PRIMER_RIGHT_9_TM=59.835
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=644
PRIMER_PAIR_PENALTY_10=0.3076
PRIMER_LEFT_10_PENALTY=0.289301
PRIMER_RIGHT_10_PENALTY=0.018344
PRIMER_LEFT_10_SEQUENCE=CATTGAGGACAACGAGTACACAG
PRIMER_RIGHT_10_SEQUENCE=GACTATGGCACAAGAACTCCATC
PRIMER_LEFT_10=1214,23
PRIMER_RIGHT_10=1661,23
PRIMER_LEFT_10_TM=59.711
PRIMER_RIGHT_10_TM=60.018
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=448
PRIMER_PAIR_PENALTY_11=0.3133
PRIMER_LEFT_11_PENALTY=0.147950
PRIMER_RIGHT_11_PENALTY=0.165371
PRIMER_LEFT_11_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_11_SEQUENCE=AAAGAAAAGTAGGTGGGCAGAAG
PRIMER_LEFT_11=1007,23
PRIMER_RIGHT_11=1910,23
PRIMER_LEFT_11_TM=59.852
PRIMER_RIGHT_11_TM=60.165
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=904
PRIMER_PAIR_PENALTY_12=0.3134
PRIMER_LEFT_12_PENALTY=0.147950
PRIMER_RIGHT_12_PENALTY=0.165486
PRIMER_LEFT_12_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_12_SEQUENCE=CTTCTGGATGATCTGAGGAAAGA
PRIMER_LEFT_12=1007,23
PRIMER_RIGHT_12=1928,23
PRIMER_LEFT_12_TM=59.852
PRIMER_RIGHT_12_TM=59.835
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=922
PRIMER_PAIR_PENALTY_13=0.3153
PRIMER_LEFT_13_PENALTY=0.229390
PRIMER_RIGHT_13_PENALTY=0.085878
PRIMER_LEFT_13_SEQUENCE=GCCCATCTACATCATCACTGAAT
PRIMER_RIGHT_13_SEQUENCE=CTGTCCACTTAATGGGAAACTTG
PRIMER_LEFT_13=980,23
PRIMER_RIGHT_13=1272,23
PRIMER_LEFT_13_TM=60.229
PRIMER_RIGHT_13_TM=59.914
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=6.9000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=293
PRIMER_PAIR_PENALTY_14=0.3161
PRIMER_LEFT_14_PENALTY=0.122456
PRIMER_RIGHT_14_PENALTY=0.193666
PRIMER_LEFT_14_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_14_SEQUENCE=TCTGGATGATCTGAGGAAAGAAA
PRIMER_LEFT_14=1285,23
PRIMER_RIGHT_14=1926,23
PRIMER_LEFT_14_TM=60.122
PRIMER_RIGHT_14_TM=60.194
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=642
PRIMER_PAIR_PENALTY_15=0.3161
PRIMER_LEFT_15_PENALTY=0.122456
PRIMER_RIGHT_15_PENALTY=0.193666
PRIMER_LEFT_15_SEQUENCE=TTAACTACGGGACATTCACCATC
PRIMER_RIGHT_15_SEQUENCE=TTCTGGATGATCTGAGGAAAGAA
PRIMER_LEFT_15=1285,23
PRIMER_RIGHT_15=1927,23
PRIMER_LEFT_15_TM=60.122
PRIMER_RIGHT_15_TM=60.194
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT2B2 Interspersed repeat MLT2B2 - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=643
PRIMER_PAIR_PENALTY_16=0.3411
PRIMER_LEFT_16_PENALTY=0.221594
PRIMER_RIGHT_16_PENALTY=0.119476
PRIMER_LEFT_16_SEQUENCE=CTGTACACGTGTCCTGTAGTTGC
PRIMER_RIGHT_16_SEQUENCE=ATTAGATAAAGTCCTGGCCCTTG
PRIMER_LEFT_16=1731,23
PRIMER_RIGHT_16=1956,23
PRIMER_LEFT_16_TM=59.778
PRIMER_RIGHT_16_TM=59.881
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=226
PRIMER_PAIR_PENALTY_17=0.3416
PRIMER_LEFT_17_PENALTY=0.147950
PRIMER_RIGHT_17_PENALTY=0.193666
PRIMER_LEFT_17_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_17_SEQUENCE=TCTGGATGATCTGAGGAAAGAAA
PRIMER_LEFT_17=1007,23
PRIMER_RIGHT_17=1926,23
PRIMER_LEFT_17_TM=59.852
PRIMER_RIGHT_17_TM=60.194
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=920
PRIMER_PAIR_PENALTY_18=0.3416
PRIMER_LEFT_18_PENALTY=0.147950
PRIMER_RIGHT_18_PENALTY=0.193666
PRIMER_LEFT_18_SEQUENCE=GGAGAATGGGAGTCTAGTGGATT
PRIMER_RIGHT_18_SEQUENCE=TTCTGGATGATCTGAGGAAAGAA
PRIMER_LEFT_18=1007,23
PRIMER_RIGHT_18=1927,23
PRIMER_LEFT_18_TM=59.852
PRIMER_RIGHT_18_TM=60.194
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=921
PRIMER_PAIR_PENALTY_19=0.3489
PRIMER_LEFT_19_PENALTY=0.229390
PRIMER_RIGHT_19_PENALTY=0.119476
PRIMER_LEFT_19_SEQUENCE=GCCCATCTACATCATCACTGAAT
PRIMER_RIGHT_19_SEQUENCE=ATTAGATAAAGTCCTGGCCCTTG
PRIMER_LEFT_19=980,23
PRIMER_RIGHT_19=1956,23
PRIMER_LEFT_19_TM=60.229
PRIMER_RIGHT_19_TM=59.881
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.5000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=977
=