PRIMER_SEQUENCE_ID=NM_005424
SEQUENCE=CGCTCGTCCTGGCTGGCCTGGGTCGGCCTCTGGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATGTGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCTTCTTCCTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCACCCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCTCGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCATCTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAGGTGACACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGCAACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTGCAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGTTGTGGGGCTGGGCGCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATGGAGGTGTCTGCCACGACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACAGGCCTGCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCCGGGGCCTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAAGAAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAGTGTCAGAATGGTGGCACTTGTGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCATGGAGTGCACTGTGAGAAGTCAGACCGGATCCCCCAGATCCTCAACATGGCCTCAGAACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAGCTGCAGGGAACCCCTTCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTCCACCAAGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTGCGGACAGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTGAAAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAGAGCCGCCAGCTTGTGGTCTCCCCGCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTCCGCCTGCACTACCGGCCCCAGGACAGTACCATGGACTGGTCGACCATTGTGGTGGACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAGGATACAGTGTTCGTGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCTCATGACCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCACTGACCGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACGGTTTCCTGCTGCGCCTGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCATCCCCCCAGGCCCGCACTGCCCTCCTGACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCGGCCTCGCCCCCTGCACACGTGCTTCTGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGGCCCTCTCAGACTCCGAGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTACGTTGTGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTGAGGAGACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGCGGGCCAGCATTCAGGGGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCAACGGGCTGCAGGCTGAGGGCCCAGTCCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATCAGCAGCTGATCCTGGCGGTGGTGGGCTCCGTGTCTGCCACCTGCCTCACCATCCTGGCCGCCCTTTTAACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGAGACGCACCTTCACCTACCAGTCAGGCTCGGGCGAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACACTTACCCGGCGGCCAAAACTGCAGCCCGAGCCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACATCACCTTTGAGGACCTCATCGGGGAGGGGAACTTCGGCCAGGTCATCCGGGCCATGATCAAGAAGGACGGGCTGAAGATGAACGCAGCCATCAAAATGCTGAAAGAGTATGCCTCTGAAAATGACCATCGTGACTTTGCGGGAGAACTGGAAGTTCTGTGCAAATTGGGGCATCACCCCAACATCATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTACTTGTATATCGCTATTGAATATGCCCCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGGTCCTAGAGACTGACCCAGCTTTTGCTCGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCTGCGTTTCGCCAGTGATGCGGCCAATGGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGGACCTGGCTGCCCGGAATGTGCTGGTCGGAGAGAACCTAGCCTCCAAGATTGCAGACTTCGGCCTTTCTCGGGGAGAGGAGGTTTATGTGAAGAAGACGATGGGGCGTCTCCCTGTGCGCTGGATGGCCATTGAGTCCCTGAACTACAGTGTCTATACCACCAAGAGTGATGTCTGGTCCTTTGGAGTCCTTCTTTGGGAGATAGTGAGCCTTGGAGGTACACCCTACTGTGGCATGACCTGTGCCGAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGCAGCCTCGAAACTGTGACGATGAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCGACCCCCCTTTGCCCAGATTGCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGAACATGTCGCTGTTTGAGAACTTCACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCCTGAGCTGCCATCCAGCCAGAACGTGGCTCTGCTGGCCGGAGCAAACTCTGCTGTCTAACCTGTGACCAGTCTGACCCTTACAGCCTCTGACTTAAGCTGCCTCAAGGAATTTTTTTAACTTAAGGGAGAAAAAAAGGGATCTGGGGATGGGGTGGGCTTAGGGGAACTGGGTTCCCATGCTTTGTAGGTGTCTCATAGCTATCCTGGGCATCCTTCTTTCTAGTTCAGCTGCCCCACAGGTGTGTTTCCCATCCCACTGCTCCCCCAACACAAACCCCCACTCCAGCTCCTTCGCTTAAGCCAGCACTCACACCACTAACATGCCCTGTTCAGCTACTCCCACTCCCGGCCTGTCATTCAGAAAAAAATAAATGTTCTAATAAGCTCCAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 43718, GC content failed 1811, low tm 9233, high tm 24642, high any compl 9, high end compl 44, high repeat similarity 55, long poly-x seq 161, ok 7763
PRIMER_RIGHT_EXPLAIN=considered 43593, GC content failed 1890, low tm 9360, high tm 24302, high end compl 9, high repeat similarity 10, long poly-x seq 195, ok 7827
PRIMER_PAIR_EXPLAIN=considered 3241, unacceptable product size 3147, high end compl 7, ok 87
PRIMER_PAIR_PENALTY=0.0977
PRIMER_LEFT_PENALTY=0.034654
PRIMER_RIGHT_PENALTY=0.063026
PRIMER_LEFT_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_SEQUENCE=GATGAACTGCTTCTCACTCAGGT
PRIMER_LEFT=2648,23
PRIMER_RIGHT=2963,23
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.937
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=316
PRIMER_PAIR_PENALTY_1=0.1658
PRIMER_LEFT_1_PENALTY=0.034654
PRIMER_RIGHT_1_PENALTY=0.131151
PRIMER_LEFT_1_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_1_SEQUENCE=ACTGCTTCTCACTCAGGTACTGC
PRIMER_LEFT_1=2648,23
PRIMER_RIGHT_1=2958,23
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=311
PRIMER_PAIR_PENALTY_2=0.1754
PRIMER_LEFT_2_PENALTY=0.034654
PRIMER_RIGHT_2_PENALTY=0.140717
PRIMER_LEFT_2_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_2_SEQUENCE=AGACTGGTCACAGGTTAGACAGC
PRIMER_LEFT_2=2648,23
PRIMER_RIGHT_2=3521,23
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=874
PRIMER_PAIR_PENALTY_3=0.2037
PRIMER_LEFT_3_PENALTY=0.063026
PRIMER_RIGHT_3_PENALTY=0.140717
PRIMER_LEFT_3_SEQUENCE=ACCTGAGTGAGAAGCAGTTCATC
PRIMER_RIGHT_3_SEQUENCE=AGACTGGTCACAGGTTAGACAGC
PRIMER_LEFT_3=2941,23
PRIMER_RIGHT_3=3521,23
PRIMER_LEFT_3_TM=59.937
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=581
PRIMER_PAIR_PENALTY_4=0.2081
PRIMER_LEFT_4_PENALTY=0.145108
PRIMER_RIGHT_4_PENALTY=0.063026
PRIMER_LEFT_4_SEQUENCE=GTATGCCTCTGAAAATGACCATC
PRIMER_RIGHT_4_SEQUENCE=GATGAACTGCTTCTCACTCAGGT
PRIMER_LEFT_4=2657,23
PRIMER_RIGHT_4=2963,23
PRIMER_LEFT_4_TM=59.855
PRIMER_RIGHT_4_TM=59.937
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=307
PRIMER_PAIR_PENALTY_5=0.2147
PRIMER_LEFT_5_PENALTY=0.145108
PRIMER_RIGHT_5_PENALTY=0.069594
PRIMER_LEFT_5_SEQUENCE=GTATGCCTCTGAAAATGACCATC
PRIMER_RIGHT_5_SEQUENCE=AAGTAACCTCGGTTCTTACAGGC
PRIMER_LEFT_5=2657,23
PRIMER_RIGHT_5=2773,23
PRIMER_LEFT_5_TM=59.855
PRIMER_RIGHT_5_TM=60.070
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=8.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=117
PRIMER_PAIR_PENALTY_6=0.2169
PRIMER_LEFT_6_PENALTY=0.034654
PRIMER_RIGHT_6_PENALTY=0.182197
PRIMER_LEFT_6_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_6_SEQUENCE=TCAGACTGGTCACAGGTTAGACA
PRIMER_LEFT_6=2648,23
PRIMER_RIGHT_6=3523,23
PRIMER_LEFT_6_TM=60.035
PRIMER_RIGHT_6_TM=59.818
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.3000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=876
PRIMER_PAIR_PENALTY_7=0.2452
PRIMER_LEFT_7_PENALTY=0.063026
PRIMER_RIGHT_7_PENALTY=0.182197
PRIMER_LEFT_7_SEQUENCE=ACCTGAGTGAGAAGCAGTTCATC
PRIMER_RIGHT_7_SEQUENCE=TCAGACTGGTCACAGGTTAGACA
PRIMER_LEFT_7=2941,23
PRIMER_RIGHT_7=3523,23
PRIMER_LEFT_7_TM=59.937
PRIMER_RIGHT_7_TM=59.818
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=583
PRIMER_PAIR_PENALTY_8=0.2524
PRIMER_LEFT_8_PENALTY=0.034654
PRIMER_RIGHT_8_PENALTY=0.217785
PRIMER_LEFT_8_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_8_SEQUENCE=TACACTTCATCGTCACAGTTTCG
PRIMER_LEFT_8=2648,23
PRIMER_RIGHT_8=3283,23
PRIMER_LEFT_8_TM=60.035
PRIMER_RIGHT_8_TM=60.218
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.3000
PRIMER_RIGHT_8_END_STABILITY=9.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=636
PRIMER_PAIR_PENALTY_9=0.2583
PRIMER_LEFT_9_PENALTY=0.223645
PRIMER_RIGHT_9_PENALTY=0.034654
PRIMER_LEFT_9_SEQUENCE=GCAACACAGTAGAAGAGTCCACC
PRIMER_RIGHT_9_SEQUENCE=TTTCAGAGGCATACTCTTTCAGC
PRIMER_LEFT_9=2221,23
PRIMER_RIGHT_9=2670,23
PRIMER_LEFT_9_TM=60.224
PRIMER_RIGHT_9_TM=60.035
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=450
PRIMER_PAIR_PENALTY_10=0.2719
PRIMER_LEFT_10_PENALTY=0.131151
PRIMER_RIGHT_10_PENALTY=0.140717
PRIMER_LEFT_10_SEQUENCE=GCAGTACCTGAGTGAGAAGCAGT
PRIMER_RIGHT_10_SEQUENCE=AGACTGGTCACAGGTTAGACAGC
PRIMER_LEFT_10=2936,23
PRIMER_RIGHT_10=3521,23
PRIMER_LEFT_10_TM=60.131
PRIMER_RIGHT_10_TM=59.859
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=52.174
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=586
PRIMER_PAIR_PENALTY_11=0.2763
PRIMER_LEFT_11_PENALTY=0.145108
PRIMER_RIGHT_11_PENALTY=0.131151
PRIMER_LEFT_11_SEQUENCE=GTATGCCTCTGAAAATGACCATC
PRIMER_RIGHT_11_SEQUENCE=ACTGCTTCTCACTCAGGTACTGC
PRIMER_LEFT_11=2657,23
PRIMER_RIGHT_11=2958,23
PRIMER_LEFT_11_TM=59.855
PRIMER_RIGHT_11_TM=60.131
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=302
PRIMER_PAIR_PENALTY_12=0.2782
PRIMER_LEFT_12_PENALTY=0.063026
PRIMER_RIGHT_12_PENALTY=0.215198
PRIMER_LEFT_12_SEQUENCE=ACCTGAGTGAGAAGCAGTTCATC
PRIMER_RIGHT_12_SEQUENCE=GCAGCTTAAGTCAGAGGCTGTAA
PRIMER_LEFT_12=2941,23
PRIMER_RIGHT_12=3549,23
PRIMER_LEFT_12_TM=59.937
PRIMER_RIGHT_12_TM=60.215
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.6000
PRIMER_RIGHT_12_END_STABILITY=6.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=609
PRIMER_PAIR_PENALTY_13=0.2808
PRIMER_LEFT_13_PENALTY=0.063026
PRIMER_RIGHT_13_PENALTY=0.217785
PRIMER_LEFT_13_SEQUENCE=ACCTGAGTGAGAAGCAGTTCATC
PRIMER_RIGHT_13_SEQUENCE=TACACTTCATCGTCACAGTTTCG
PRIMER_LEFT_13=2941,23
PRIMER_RIGHT_13=3283,23
PRIMER_LEFT_13_TM=59.937
PRIMER_RIGHT_13_TM=60.218
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER1B Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=6.6000
PRIMER_RIGHT_13_END_STABILITY=9.0000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=343
PRIMER_PAIR_PENALTY_14=0.2858
PRIMER_LEFT_14_PENALTY=0.145108
PRIMER_RIGHT_14_PENALTY=0.140717
PRIMER_LEFT_14_SEQUENCE=GTATGCCTCTGAAAATGACCATC
PRIMER_RIGHT_14_SEQUENCE=AGACTGGTCACAGGTTAGACAGC
PRIMER_LEFT_14=2657,23
PRIMER_RIGHT_14=3521,23
PRIMER_LEFT_14_TM=59.855
PRIMER_RIGHT_14_TM=59.859
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=865
PRIMER_PAIR_PENALTY_15=0.2932
PRIMER_LEFT_15_PENALTY=0.223645
PRIMER_RIGHT_15_PENALTY=0.069594
PRIMER_LEFT_15_SEQUENCE=GCAACACAGTAGAAGAGTCCACC
PRIMER_RIGHT_15_SEQUENCE=AAGTAACCTCGGTTCTTACAGGC
PRIMER_LEFT_15=2221,23
PRIMER_RIGHT_15=2773,23
PRIMER_LEFT_15_TM=60.224
PRIMER_RIGHT_15_TM=60.070
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=9.4000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=553
PRIMER_PAIR_PENALTY_16=0.2952
PRIMER_LEFT_16_PENALTY=0.034654
PRIMER_RIGHT_16_PENALTY=0.260543
PRIMER_LEFT_16_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_16_SEQUENCE=CAATCTTGGAGGCTAGGTTCTCT
PRIMER_LEFT_16=2648,23
PRIMER_RIGHT_16=3021,23
PRIMER_LEFT_16_TM=60.035
PRIMER_RIGHT_16_TM=60.261
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.3000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=374
PRIMER_PAIR_PENALTY_17=0.3133
PRIMER_LEFT_17_PENALTY=0.131151
PRIMER_RIGHT_17_PENALTY=0.182197
PRIMER_LEFT_17_SEQUENCE=GCAGTACCTGAGTGAGAAGCAGT
PRIMER_RIGHT_17_SEQUENCE=TCAGACTGGTCACAGGTTAGACA
PRIMER_LEFT_17=2936,23
PRIMER_RIGHT_17=3523,23
PRIMER_LEFT_17_TM=60.131
PRIMER_RIGHT_17_TM=59.818
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=6.4000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=588
PRIMER_PAIR_PENALTY_18=0.3273
PRIMER_LEFT_18_PENALTY=0.145108
PRIMER_RIGHT_18_PENALTY=0.182197
PRIMER_LEFT_18_SEQUENCE=GTATGCCTCTGAAAATGACCATC
PRIMER_RIGHT_18_SEQUENCE=TCAGACTGGTCACAGGTTAGACA
PRIMER_LEFT_18=2657,23
PRIMER_RIGHT_18=3523,23
PRIMER_LEFT_18_TM=59.855
PRIMER_RIGHT_18_TM=59.818
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=867
PRIMER_PAIR_PENALTY_19=0.3359
PRIMER_LEFT_19_PENALTY=0.034654
PRIMER_RIGHT_19_PENALTY=0.301227
PRIMER_LEFT_19_SEQUENCE=GCTGAAAGAGTATGCCTCTGAAA
PRIMER_RIGHT_19_SEQUENCE=TAAGTCAGAGGCTGTAAGGGTCA
PRIMER_LEFT_19=2648,23
PRIMER_RIGHT_19=3543,23
PRIMER_LEFT_19_TM=60.035
PRIMER_RIGHT_19_TM=60.301
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=8.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=896
=