PRIMER_SEQUENCE_ID=NM_005433
SEQUENCE=AGATTTGATAATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATATGATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGTATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGATTGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGTGGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTACACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCTCAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAGGTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACGAAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAAGCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCTGAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTTAAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATTGCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCTAATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTAATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCTCCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATTCTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAAGTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCCCTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGAGAAAATTTATAATTCAAGTAGCCTATTTTATATGCACAAATCTGCCAAAATATAAAGAACTTGTGTAGATTTTCTACAGGAATCAAAAGAAGAAAATCTTCTTTACTCTGCATGTTTTTAATGGTAAACTGGAATCCCAGATATGGTTGCACAAAACCACTTTTTTTTCCCCAAGTATTAAACTCTAATGTACCAATGATGAATTTATCAGCGTATTTCAGGGTCCAAACAAAATAGAGCTAAGATACTGATGACAGTGTGGGTGACAGCATGGTAATGAAGGACAGTGAGGCTCCTGCTTATTTATAAATCATTTCCTTTCTTTTTTTCCCCAAAGTCAGAATTGCTCAAAGAAAATTATTTATTGTTACAGATAAAACTTGAGAGATAAAAAGCTATACCATAATAAAATCTAAAATTAAGGAATATCATGGGACCAAATAATTCCATTCCAGTTTTTTAAAGTTTCTTGCATTTATTATTCTCAAAAGTTTTTTCTAAGTTAAACAGTCAGTATGCAATCTTAATATATGCTTTCTTTTGCATGGACATGGGCCAGGTTTTTCAAAAGGAATATAAACAGGATCTCAAACTTGATTAAATGTTAGACCACAGAAGTGGAATTTGAAAGTATAATGCAGTACATTAATATTCATGTTCATGGAACTGAAAGAATAAGAACTTTTTCACTTCAGTCCTTTTCTGAAGAGTTTGACTTAGAATAATGAAGGTAACTAGAAAGTGAGTTAATCTTGTATGAGGTTGCATTGATTTTTTAAGGCAATATATAATTGAAACTACTGTCCAATCAAAGGGGAAATGTTTTGATCTTTAGATAGCATGCAAAGTAAGACCCAGCATTTTAAAAGCCCTTTTTAAAAACTAGACTTCGTACTGTGAGTATTGCTTATATGTCCTTATGGGGATGGGTGCCACAAATAGAAAATATGACCAGATCAGGGACTTGAATGCACTTTTGCTCATGGTGAATATAGATGAACAGAGAGGAAAATGTATTTAAAAGAAATACGAGAAAAGAAAATGTGAAAGTTTTACAAGTTAGAGGGATGGAAGGTAATGTTTAATGTTGATGTCATGGAGTGACAGAATGGCTTTGCTGGCACTCAGAGCTCCTCACTTAGCTATATTCTGAGACTTTGAAGAGTTATAAAGTATAACTATAAAACTAATTTTTCTTACACACTAAATGGGTATTTGTTCAAAATAATGAAGTTATGGCTTCACATTCATTGCAGTGGGATATGGTTTTTATGTAAAACATTTTTAGAACTCCAGTTTTCAAATCATGTTTGAATCTACATTCACTTTTTTTTGTTTTCTTTTTTGAGACGGAGTCTCGCTCTGCCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGTTCACACCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGTGCCCACCACCACGCCTGGCTAGTTTTTTGTATTTTTAGTAGAGACGCAGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCAGCCTACATTCACTTCTAAAGTCTATGTAATGGTGGTCATTTTTTCCCTTTTAGAATACATTAAATGGTTGATTTGGGGAGGAAAACTTATTCTGAATATTAACGGTGGTGAAAAGGGGACAGTTTTTACCCTAAAGTGCAAAAGTGAAACATACAAAATAAGACTAATTTTTAAGAGTAACTCAGTAATTTCAAAATACAGATTTGAATAGCAGCATTAGTGGTTTGAGTGTCTAGCAAAGGAAAAATTGATGAATAAAATGAAGGTCTGGTGTATATGTTTTAAAATACTCTCATATAGTCACACTTTAAATTAAGCCTTATATTAGGCCCCTCTATTTTCAGGATATAATTCTTAACTATCATTATTTACCTGATTTTAATCATCAGATTCGAAATTCTGTGCCATGGCGTATATGTTCAAATTCAAACCATTTTTAAAATGTGAAGATGGACTTCATGCAAGTTGGCAGTGGTTCTGGTACTAAAAATTGTGGTTGTTTTTTCTGTTTACGTAACCTGCTTAGTATTGACACTCTCTACCAAGAGGGTCTTCCTAAGAAGAGTGCTGTCATTATTTCCTCTTATCAACAACTTGTGACATGAGATTTTTTAAGGGCTTTATGTGAACTATGATATTGTAATTTTTCTAAGCATATTCAAAAGGGTGACAAAATTACGTTTATGTACTAAATCTAATCAGGAAAGTAAGGCAGGAAAAGTTGATGGTATTCATTAGGTTTTAACTGAATGGAGCAGTTCCTTATATAATAACAATTGTATAGTAGGGATAAAACACTAACTTAATGTGTATTCATTTTAAATTGTTCTGTATTTTTAAATTGCCAAGAAAAACAACTTTGTAAATTTGGAGATATTTTCCAACAGCTTTTCGTCTTCAGTGTCTTAATGTGGAAGTTAACCCTTACCAAAAAAGGAAGTTGGCAAAAACAGCCTTCTAGCACACTTTTTTAAATGAATAATGGTAGCCTAAACTTAATATTTTTATAAAGTATTGTAATATTGTTTTGTGGATAATTGAAATAAAAAGTTCTCATTGAATGCACCTATTAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 48843, GC content failed 3213, low tm 29534, high tm 6567, high any compl 4, high end compl 34, high repeat similarity 32, long poly-x seq 276, ok 9183
PRIMER_RIGHT_EXPLAIN=considered 48738, GC content failed 3551, low tm 29328, high tm 6538, high any compl 1, high end compl 6, high repeat similarity 7, long poly-x seq 292, ok 9015
PRIMER_PAIR_EXPLAIN=considered 627, unacceptable product size 566, high any compl 1, high end compl 1, ok 59
PRIMER_PAIR_PENALTY=0.0148
PRIMER_LEFT_PENALTY=0.010384
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=TAAGGCAGGAAAAGTTGATGGTA
PRIMER_RIGHT_SEQUENCE=AGTGTGCTAGAAGGCTGTTTTTG
PRIMER_LEFT=3961,23
PRIMER_RIGHT=4220,23
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MER4C a consensus
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.0451
PRIMER_LEFT_1_PENALTY=0.009608
PRIMER_RIGHT_1_PENALTY=0.035468
PRIMER_LEFT_1_SEQUENCE=GATGAATCTGTGTTGGAAGAAGG
PRIMER_RIGHT_1_SEQUENCE=ATCTGGGATTCCAGTTTACCATT
PRIMER_LEFT_1=1521,23
PRIMER_RIGHT_1=1771,23
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse PAB Human pseudoautosomal boundary-like sequence.
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.0451
PRIMER_LEFT_2_PENALTY=0.009608
PRIMER_RIGHT_2_PENALTY=0.035468
PRIMER_LEFT_2_SEQUENCE=ATGAATCTGTGTTGGAAGAAGGA
PRIMER_RIGHT_2_SEQUENCE=ATCTGGGATTCCAGTTTACCATT
PRIMER_LEFT_2=1522,23
PRIMER_RIGHT_2=1771,23
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=59.965
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.0560
PRIMER_LEFT_3_PENALTY=0.046368
PRIMER_RIGHT_3_PENALTY=0.009608
PRIMER_LEFT_3_SEQUENCE=CACAACCAGAGCACAATTTGATA
PRIMER_RIGHT_3_SEQUENCE=TCCTTCTTCCAACACAGATTCAT
PRIMER_LEFT_3=681,23
PRIMER_RIGHT_3=1544,23
PRIMER_LEFT_3_TM=60.046
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MIR2 Repetitive element - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=864
PRIMER_PAIR_PENALTY_4=0.0574
PRIMER_LEFT_4_PENALTY=0.010990
PRIMER_RIGHT_4_PENALTY=0.046368
PRIMER_LEFT_4_SEQUENCE=CTCCAGAGCCTGTCAGTACAAGT
PRIMER_RIGHT_4_SEQUENCE=TATCAAATTGTGCTCTGGTTGTG
PRIMER_LEFT_4=71,23
PRIMER_RIGHT_4=703,23
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=60.046
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=633
PRIMER_PAIR_PENALTY_5=0.0574
PRIMER_LEFT_5_PENALTY=0.010990
PRIMER_RIGHT_5_PENALTY=0.046368
PRIMER_LEFT_5_SEQUENCE=ACTCCAGAGCCTGTCAGTACAAG
PRIMER_RIGHT_5_SEQUENCE=TATCAAATTGTGCTCTGGTTGTG
PRIMER_LEFT_5=70,23
PRIMER_RIGHT_5=703,23
PRIMER_LEFT_5_TM=59.989
PRIMER_RIGHT_5_TM=60.046
PRIMER_LEFT_5_GC_PERCENT=52.174
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=7.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=634
PRIMER_PAIR_PENALTY_6=0.0605
PRIMER_LEFT_6_PENALTY=0.009608
PRIMER_RIGHT_6_PENALTY=0.050923
PRIMER_LEFT_6_SEQUENCE=GATGAATCTGTGTTGGAAGAAGG
PRIMER_RIGHT_6_SEQUENCE=ATCAAAACATTTCCCCTTTGATT
PRIMER_LEFT_6=1521,23
PRIMER_RIGHT_6=2458,23
PRIMER_LEFT_6_TM=59.990
PRIMER_RIGHT_6_TM=59.949
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=30.435
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse PAB Human pseudoautosomal boundary-like sequence.
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=938
PRIMER_PAIR_PENALTY_7=0.0605
PRIMER_LEFT_7_PENALTY=0.009608
PRIMER_RIGHT_7_PENALTY=0.050923
PRIMER_LEFT_7_SEQUENCE=ATGAATCTGTGTTGGAAGAAGGA
PRIMER_RIGHT_7_SEQUENCE=ATCAAAACATTTCCCCTTTGATT
PRIMER_LEFT_7=1522,23
PRIMER_RIGHT_7=2458,23
PRIMER_LEFT_7_TM=59.990
PRIMER_RIGHT_7_TM=59.949
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=30.435
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.9000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=937
PRIMER_PAIR_PENALTY_8=0.0618
PRIMER_LEFT_8_PENALTY=0.010990
PRIMER_RIGHT_8_PENALTY=0.050782
PRIMER_LEFT_8_SEQUENCE=CTCCAGAGCCTGTCAGTACAAGT
PRIMER_RIGHT_8_SEQUENCE=TCGGGATATAACCATTCTTTCCT
PRIMER_LEFT_8=71,23
PRIMER_RIGHT_8=439,23
PRIMER_LEFT_8_TM=59.989
PRIMER_RIGHT_8_TM=60.051
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=369
PRIMER_PAIR_PENALTY_9=0.0618
PRIMER_LEFT_9_PENALTY=0.010990
PRIMER_RIGHT_9_PENALTY=0.050782
PRIMER_LEFT_9_SEQUENCE=ACTCCAGAGCCTGTCAGTACAAG
PRIMER_RIGHT_9_SEQUENCE=TCGGGATATAACCATTCTTTCCT
PRIMER_LEFT_9=70,23
PRIMER_RIGHT_9=439,23
PRIMER_LEFT_9_TM=59.989
PRIMER_RIGHT_9_TM=60.051
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=370
PRIMER_PAIR_PENALTY_10=0.0681
PRIMER_LEFT_10_PENALTY=0.063624
PRIMER_RIGHT_10_PENALTY=0.004430
PRIMER_LEFT_10_SEQUENCE=TGATTTGGGGAGGAAAACTTATT
PRIMER_RIGHT_10_SEQUENCE=AGTGTGCTAGAAGGCTGTTTTTG
PRIMER_LEFT_10=3322,23
PRIMER_RIGHT_10=4220,23
PRIMER_LEFT_10_TM=60.064
PRIMER_RIGHT_10_TM=59.996
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_10_END_STABILITY=6.2000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=899
PRIMER_PAIR_PENALTY_11=0.0769
PRIMER_LEFT_11_PENALTY=0.026007
PRIMER_RIGHT_11_PENALTY=0.050923
PRIMER_LEFT_11_SEQUENCE=AGACCACAGAAGTGGAATTTGAA
PRIMER_RIGHT_11_SEQUENCE=ATCAAAACATTTCCCCTTTGATT
PRIMER_LEFT_11=2236,23
PRIMER_RIGHT_11=2458,23
PRIMER_LEFT_11_TM=60.026
PRIMER_RIGHT_11_TM=59.949
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=30.435
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.3000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=223
PRIMER_PAIR_PENALTY_12=0.0792
PRIMER_LEFT_12_PENALTY=0.069594
PRIMER_RIGHT_12_PENALTY=0.009608
PRIMER_LEFT_12_SEQUENCE=GAACTAGTAACAAAGGGCCGAGT
PRIMER_RIGHT_12_SEQUENCE=TCCTTCTTCCAACACAGATTCAT
PRIMER_LEFT_12=1399,23
PRIMER_RIGHT_12=1544,23
PRIMER_LEFT_12_TM=60.070
PRIMER_RIGHT_12_TM=59.990
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse PTR5 Human PTR5 mRNA for repetitive sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MIR2 Repetitive element - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=146
PRIMER_PAIR_PENALTY_13=0.0840
PRIMER_LEFT_13_PENALTY=0.074396
PRIMER_RIGHT_13_PENALTY=0.009608
PRIMER_LEFT_13_SEQUENCE=GCAATCAAAACACTAAAACCAGG
PRIMER_RIGHT_13_SEQUENCE=TCCTTCTTCCAACACAGATTCAT
PRIMER_LEFT_13=916,23
PRIMER_RIGHT_13=1544,23
PRIMER_LEFT_13_TM=59.926
PRIMER_RIGHT_13_TM=59.990
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MIR2 Repetitive element - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=6.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=629
PRIMER_PAIR_PENALTY_14=0.0854
PRIMER_LEFT_14_PENALTY=0.010990
PRIMER_RIGHT_14_PENALTY=0.074396
PRIMER_LEFT_14_SEQUENCE=CTCCAGAGCCTGTCAGTACAAGT
PRIMER_RIGHT_14_SEQUENCE=CCTGGTTTTAGTGTTTTGATTGC
PRIMER_LEFT_14=71,23
PRIMER_RIGHT_14=938,23
PRIMER_LEFT_14_TM=59.989
PRIMER_RIGHT_14_TM=59.926
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=868
PRIMER_PAIR_PENALTY_15=0.0854
PRIMER_LEFT_15_PENALTY=0.010990
PRIMER_RIGHT_15_PENALTY=0.074396
PRIMER_LEFT_15_SEQUENCE=ACTCCAGAGCCTGTCAGTACAAG
PRIMER_RIGHT_15_SEQUENCE=CCTGGTTTTAGTGTTTTGATTGC
PRIMER_LEFT_15=70,23
PRIMER_RIGHT_15=938,23
PRIMER_LEFT_15_TM=59.989
PRIMER_RIGHT_15_TM=59.926
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.7000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=869
PRIMER_PAIR_PENALTY_16=0.0864
PRIMER_LEFT_16_PENALTY=0.035468
PRIMER_RIGHT_16_PENALTY=0.050923
PRIMER_LEFT_16_SEQUENCE=AAACTGGAATCCCAGATATGGTT
PRIMER_RIGHT_16_SEQUENCE=ATCAAAACATTTCCCCTTTGATT
PRIMER_LEFT_16=1755,23
PRIMER_RIGHT_16=2458,23
PRIMER_LEFT_16_TM=59.965
PRIMER_RIGHT_16_TM=59.949
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=30.435
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.9000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=704
PRIMER_PAIR_PENALTY_17=0.0871
PRIMER_LEFT_17_PENALTY=0.010990
PRIMER_RIGHT_17_PENALTY=0.076141
PRIMER_LEFT_17_SEQUENCE=CTCCAGAGCCTGTCAGTACAAGT
PRIMER_RIGHT_17_SEQUENCE=ATTGCTCGGGATATAACCATTCT
PRIMER_LEFT_17=71,23
PRIMER_RIGHT_17=444,23
PRIMER_LEFT_17_TM=59.989
PRIMER_RIGHT_17_TM=60.076
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=10.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=374
PRIMER_PAIR_PENALTY_18=0.0871
PRIMER_LEFT_18_PENALTY=0.010990
PRIMER_RIGHT_18_PENALTY=0.076141
PRIMER_LEFT_18_SEQUENCE=ACTCCAGAGCCTGTCAGTACAAG
PRIMER_RIGHT_18_SEQUENCE=ATTGCTCGGGATATAACCATTCT
PRIMER_LEFT_18=70,23
PRIMER_RIGHT_18=444,23
PRIMER_LEFT_18_TM=59.989
PRIMER_RIGHT_18_TM=60.076
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=10.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.7000
PRIMER_RIGHT_18_END_STABILITY=6.6000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=375
PRIMER_PAIR_PENALTY_19=0.0972
PRIMER_LEFT_19_PENALTY=0.050782
PRIMER_RIGHT_19_PENALTY=0.046368
PRIMER_LEFT_19_SEQUENCE=AGGAAAGAATGGTTATATCCCGA
PRIMER_RIGHT_19_SEQUENCE=TATCAAATTGTGCTCTGGTTGTG
PRIMER_LEFT_19=417,23
PRIMER_RIGHT_19=703,23
PRIMER_LEFT_19_TM=60.051
PRIMER_RIGHT_19_TM=60.046
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=11.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=287
=