PRIMER_SEQUENCE_ID=NM_005592
SEQUENCE=TGCGTTGTCCAGAAGGAACTTCGTCCTGCGTGAGCCTGGATTAATCATGAGAGAGCTCGTCAACATTCCACTGGTACATATTCTTACTCTGGTTGCCTTCAGCGGAACTGAGAAACTTCCAAAAGCTCCTGTCATCACCACTCCTCTTGAAACAGTGGATGCCTTAGTTGAAGAAGTGGCTACTTTCATGTGTGCAGTGGAATCCTACCCCCAGCCTGAGATTTCCTGGACTAGAAATAAAATTCTCATTAAACTCTTTGACACCCGGTACAGCATCCGGGAGAATGGGCAGCTCCTCACCATCCTGAGTGTGGAAGACAGTGATGATGGCATTTACTGCTGCACGGCCAACAATGGTGTGGGAGGAGCTGTGGAGAGTTGTGGAGCCCTGCAAGTGAAGATGAAACCTAAAATAACTCGCCCTCCCATAAATGTGAAAATAATAGAGGGATTAAAAGCAGTCCTACCATGTACTACAATGGGTAATCCCAAACCATCAGTGTCTTGGATAAAGGGAGACAGCCCTCTCAGGGAAAATTCCCGAATTGCAGTTCTTGAATCTGGGAGCTTGAGGATTCATAACGTACAAAAGGAAGATGCAGGACAGTATCGATGTGTGGCAAAAAACAGCCTCGGGACAGCATATTCCAAAGTGGTGAAGCTGGAAGTTGAGGTTTTTGCCAGGATCCTGCGGGCTCCTGAATCCCACAATGTCACCTTTGGCTCCTTTGTGACCCTGCACTGTACAGCAACAGGCATTCCTGTCCCCACCATCACCTGGATTGAAAACGGAAATGCTGTTTCTTCTGGGTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTGCAGCTGTTTATCACCAAGCCAGGACTCTACACATGCATAGCTACCAATAAGCATGGGGAGAAGTTCAGTACTGCCAAGGCTGCAGCCACCATCAGCATAGCAGAATGGAGTAAACCACAGAAAGATAACAAAGGCTACTGCGCCCAGTACAGAGGGGAGGTGTGTAATGCAGTCCTGGCAAAAGATGCTCTTGTTTTTCTCAACACCTCCTATGCGGACCCTGAGGAGGCCCAAGAGCTACTGGTCCACACGGCCTGGAATGAACTGAAAGTAGTGAGCCCAGTCTGCCGGCCAGCTGCTGAGGCTTTGTTGTGTAACCACATCTTCCAGGAGTGCAGTCCTGGAGTAGTGCCTACTCCTATTCCCATTTGCAGAGAGTACTGCTTGGCAGTAAAGGAGCTCTTCTGCGCAAAAGAATGGCTGGTAATGGAAGAGAAGACCCACAGAGGACTCTACAGATCCGAGATGCATTTGCTGTCCGTGCCAGAATGCAGCAAGCTTCCCAGCATGCATTGGGACCCCACGGCCTGTGCCAGACTGCCACATCTAGATTATAACAAAGAAAACCTAAAAACATTCCCACCAATGACGTCCTCAAAGCCAAGTGTGGACATTCCAAATCTGCCTTCCTCCTCCTCTTCTTCCTTCTCTGTCTCACCTACATACTCCATGACTGTAATAATCTCCATCATGTCCAGCTTTGCAATATTTGTGCTTCTTACCATAACTACTCTCTATTGCTGCCGAAGAAGAAAACAATGGAAAAATAAGAAAAGAGAATCAGCAGCAGTAACCCTCACCACACTGCCTTCTGAGCTCTTACTAGATAGACTTCATCCCAACCCCATGTACCAGAGGATGCCGCTCCTTCTGAACCCCAAATTGCTCAGCCTGGAGTATCCAAGGAATAACATTGAATATGTGAGAGACATCGGAGAGGGAGCGTTTGGAAGGGTGTTTCAAGCAAGGGCACCAGGCTTACTTCCCTATGAACCTTTCACTATGGTGGCAGTAAAGATGCTCAAAGAAGAAGCCTCGGCAGATATGCAAGCGGACTTTCAGAGGGAGGCAGCCCTCATGGCAGAATTTGACAACCCTAACATTGTGAAGCTATTAGGAGTGTGTGCTGTCGGGAAGCCAATGTGCCTGCTCTTTGAATACATGGCCTATGGTGACCTCAATGAGTTCCTCCGCAGCATGTCCCCTCACACCGTGTGCAGCCTCAGTCACAGTGACTTGTCTATGAGGGCTCAGGTCTCCAGCCCTGGGCCCCCACCCCTCTCCTGTGCTGAGCAGCTTTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCTTACCTCTCAGAACGTAAGTTTGTTCACCGAGATTTAGCCACCAGGAACTGCCTGGTGGGCGAGAACATGGTGGTGAAAATTGCCGACTTTGGCCTCTCCAGGAACATCTACTCAGCAGACTACTACAAAGCTAATGAAAACGACGCTATCCCTATCCGTTGGATGCCACCAGAGTCCATTTTTTATAACCGCTACACTACAGAGTCTGATGTGTGGGCCTATGGCGTGGTCCTCTGGGAGATCTTCTCCTATGGCCTGCAGCCCTACTATGGGATGGCCCATGAGGAGGTCATTTACTACGTGCGAGATGGCAACATCCTCTCCTGCCCTGAGAACTGCCCCGTGGAGCTGTACAATCTCATGCGTCTATGTTGGAGCAAGCTGCCTGCAGACAGACCCAGTTTCACCAGTATTCACCGAATTCTGGAACGCATGTGTGAGAGGGCAGAGGGAACTGTGAGTGTCTAAGGTTGAAGAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 30474, GC content failed 255, low tm 12697, high tm 10498, high any compl 6, high end compl 23, high repeat similarity 53, long poly-x seq 42, ok 6900
PRIMER_RIGHT_EXPLAIN=considered 30474, GC content failed 255, low tm 12607, high tm 10580, high any compl 2, high end compl 4, high repeat similarity 9, long poly-x seq 42, ok 6975
PRIMER_PAIR_EXPLAIN=considered 582, unacceptable product size 521, high end compl 9, ok 52
PRIMER_PAIR_PENALTY=0.0339
PRIMER_LEFT_PENALTY=0.030877
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=CAGGCTTACTTCCCTATGAACCT
PRIMER_RIGHT_SEQUENCE=ATAGGCCATGTATTCAAAGAGCA
PRIMER_LEFT=1829,23
PRIMER_RIGHT=2025,23
PRIMER_LEFT_TM=60.031
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.0339
PRIMER_LEFT_1_PENALTY=0.030877
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=CAGGCTTACTTCCCTATGAACCT
PRIMER_RIGHT_1_SEQUENCE=CATAGGCCATGTATTCAAAGAGC
PRIMER_LEFT_1=1829,23
PRIMER_RIGHT_1=2026,23
PRIMER_LEFT_1_TM=60.031
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.0594
PRIMER_LEFT_2_PENALTY=0.056374
PRIMER_RIGHT_2_PENALTY=0.003045
PRIMER_LEFT_2_SEQUENCE=TGTTGTGTAACCACATCTTCCAG
PRIMER_RIGHT_2_SEQUENCE=ATAGGCCATGTATTCAAAGAGCA
PRIMER_LEFT_2=1163,23
PRIMER_RIGHT_2=2025,23
PRIMER_LEFT_2_TM=59.944
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=863
PRIMER_PAIR_PENALTY_3=0.0594
PRIMER_LEFT_3_PENALTY=0.056374
PRIMER_RIGHT_3_PENALTY=0.003045
PRIMER_LEFT_3_SEQUENCE=TGTTGTGTAACCACATCTTCCAG
PRIMER_RIGHT_3_SEQUENCE=CATAGGCCATGTATTCAAAGAGC
PRIMER_LEFT_3=1163,23
PRIMER_RIGHT_3=2026,23
PRIMER_LEFT_3_TM=59.944
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=864
PRIMER_PAIR_PENALTY_4=0.0721
PRIMER_LEFT_4_PENALTY=0.039531
PRIMER_RIGHT_4_PENALTY=0.032614
PRIMER_LEFT_4_SEQUENCE=AAGATGCAGGACAGTATCGATGT
PRIMER_RIGHT_4_SEQUENCE=CACTCGGTCTTTCACACTCTCTT
PRIMER_LEFT_4=593,23
PRIMER_RIGHT_4=840,23
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=59.967
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=248
PRIMER_PAIR_PENALTY_5=0.0745
PRIMER_LEFT_5_PENALTY=0.041875
PRIMER_RIGHT_5_PENALTY=0.032614
PRIMER_LEFT_5_SEQUENCE=CTCATTAAACTCTTTGACACCCG
PRIMER_RIGHT_5_SEQUENCE=CACTCGGTCTTTCACACTCTCTT
PRIMER_LEFT_5=244,23
PRIMER_RIGHT_5=840,23
PRIMER_LEFT_5_TM=60.042
PRIMER_RIGHT_5_TM=59.967
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=2.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, KER Human K element interspersed repeat DNA
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=11.1000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=597
PRIMER_PAIR_PENALTY_6=0.0810
PRIMER_LEFT_6_PENALTY=0.048411
PRIMER_RIGHT_6_PENALTY=0.032614
PRIMER_LEFT_6_SEQUENCE=TTGAAACAGTGGATGCCTTAGTT
PRIMER_RIGHT_6_SEQUENCE=CACTCGGTCTTTCACACTCTCTT
PRIMER_LEFT_6=146,23
PRIMER_RIGHT_6=840,23
PRIMER_LEFT_6_TM=60.048
PRIMER_RIGHT_6_TM=59.967
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=695
PRIMER_PAIR_PENALTY_7=0.0814
PRIMER_LEFT_7_PENALTY=0.041875
PRIMER_RIGHT_7_PENALTY=0.039531
PRIMER_LEFT_7_SEQUENCE=CTCATTAAACTCTTTGACACCCG
PRIMER_RIGHT_7_SEQUENCE=ACATCGATACTGTCCTGCATCTT
PRIMER_LEFT_7=244,23
PRIMER_RIGHT_7=615,23
PRIMER_LEFT_7_TM=60.042
PRIMER_RIGHT_7_TM=60.040
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, KER Human K element interspersed repeat DNA
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, KER Human K element interspersed repeat DNA
PRIMER_LEFT_7_END_STABILITY=11.1000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=372
PRIMER_PAIR_PENALTY_8=0.0873
PRIMER_LEFT_8_PENALTY=0.056374
PRIMER_RIGHT_8_PENALTY=0.030877
PRIMER_LEFT_8_SEQUENCE=TGTTGTGTAACCACATCTTCCAG
PRIMER_RIGHT_8_SEQUENCE=AGGTTCATAGGGAAGTAAGCCTG
PRIMER_LEFT_8=1163,23
PRIMER_RIGHT_8=1851,23
PRIMER_LEFT_8_TM=59.944
PRIMER_RIGHT_8_TM=60.031
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=689
PRIMER_PAIR_PENALTY_9=0.0879
PRIMER_LEFT_9_PENALTY=0.048411
PRIMER_RIGHT_9_PENALTY=0.039531
PRIMER_LEFT_9_SEQUENCE=TTGAAACAGTGGATGCCTTAGTT
PRIMER_RIGHT_9_SEQUENCE=ACATCGATACTGTCCTGCATCTT
PRIMER_LEFT_9=146,23
PRIMER_RIGHT_9=615,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=60.040
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=470
PRIMER_PAIR_PENALTY_10=0.0893
PRIMER_LEFT_10_PENALTY=0.086226
PRIMER_RIGHT_10_PENALTY=0.003045
PRIMER_LEFT_10_SEQUENCE=ACCAGGCTTACTTCCCTATGAAC
PRIMER_RIGHT_10_SEQUENCE=CATAGGCCATGTATTCAAAGAGC
PRIMER_LEFT_10=1827,23
PRIMER_RIGHT_10=2026,23
PRIMER_LEFT_10_TM=59.914
PRIMER_RIGHT_10_TM=60.003
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=200
PRIMER_PAIR_PENALTY_11=0.0903
PRIMER_LEFT_11_PENALTY=0.048411
PRIMER_RIGHT_11_PENALTY=0.041875
PRIMER_LEFT_11_SEQUENCE=TTGAAACAGTGGATGCCTTAGTT
PRIMER_RIGHT_11_SEQUENCE=CGGGTGTCAAAGAGTTTAATGAG
PRIMER_LEFT_11=146,23
PRIMER_RIGHT_11=266,23
PRIMER_LEFT_11_TM=60.048
PRIMER_RIGHT_11_TM=60.042
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=121
PRIMER_PAIR_PENALTY_12=0.1123
PRIMER_LEFT_12_PENALTY=0.030877
PRIMER_RIGHT_12_PENALTY=0.081398
PRIMER_LEFT_12_SEQUENCE=CAGGCTTACTTCCCTATGAACCT
PRIMER_RIGHT_12_SEQUENCE=TACTGGTGAAACTGGGTCTGTCT
PRIMER_LEFT_12=1829,23
PRIMER_RIGHT_12=2599,23
PRIMER_LEFT_12_TM=60.031
PRIMER_RIGHT_12_TM=60.081
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=771
PRIMER_PAIR_PENALTY_13=0.1134
PRIMER_LEFT_13_PENALTY=0.082508
PRIMER_RIGHT_13_PENALTY=0.030877
PRIMER_LEFT_13_SEQUENCE=ATGTCCAGCTTTGCAATATTTGT
PRIMER_RIGHT_13_SEQUENCE=AGGTTCATAGGGAAGTAAGCCTG
PRIMER_LEFT_13=1546,23
PRIMER_RIGHT_13=1851,23
PRIMER_LEFT_13_TM=59.917
PRIMER_RIGHT_13_TM=60.031
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=20.00, MER6 Nonautonomous DNA transposon
PRIMER_LEFT_13_END_STABILITY=7.0000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=306
PRIMER_PAIR_PENALTY_14=0.1164
PRIMER_LEFT_14_PENALTY=0.030877
PRIMER_RIGHT_14_PENALTY=0.085505
PRIMER_LEFT_14_SEQUENCE=CAGGCTTACTTCCCTATGAACCT
PRIMER_RIGHT_14_SEQUENCE=GAATACTGGTGAAACTGGGTCTG
PRIMER_LEFT_14=1829,23
PRIMER_RIGHT_14=2602,23
PRIMER_LEFT_14_TM=60.031
PRIMER_RIGHT_14_TM=59.914
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_14=774
PRIMER_PAIR_PENALTY_15=0.1164
PRIMER_LEFT_15_PENALTY=0.030877
PRIMER_RIGHT_15_PENALTY=0.085505
PRIMER_LEFT_15_SEQUENCE=CAGGCTTACTTCCCTATGAACCT
PRIMER_RIGHT_15_SEQUENCE=TGAATACTGGTGAAACTGGGTCT
PRIMER_LEFT_15=1829,23
PRIMER_RIGHT_15=2603,23
PRIMER_LEFT_15_TM=60.031
PRIMER_RIGHT_15_TM=59.914
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_LEFT_15_END_STABILITY=7.9000
PRIMER_RIGHT_15_END_STABILITY=7.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=775
PRIMER_PAIR_PENALTY_16=0.1225
PRIMER_LEFT_16_PENALTY=0.119476
PRIMER_RIGHT_16_PENALTY=0.003045
PRIMER_LEFT_16_SEQUENCE=AACCTAAAAACATTCCCACCAAT
PRIMER_RIGHT_16_SEQUENCE=ATAGGCCATGTATTCAAAGAGCA
PRIMER_LEFT_16=1420,23
PRIMER_RIGHT_16=2025,23
PRIMER_LEFT_16_TM=59.881
PRIMER_RIGHT_16_TM=60.003
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER30 Nonautonomous DNA transposon
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_LEFT_16_END_STABILITY=8.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=606
PRIMER_PAIR_PENALTY_17=0.1225
PRIMER_LEFT_17_PENALTY=0.119476
PRIMER_RIGHT_17_PENALTY=0.003045
PRIMER_LEFT_17_SEQUENCE=AACCTAAAAACATTCCCACCAAT
PRIMER_RIGHT_17_SEQUENCE=CATAGGCCATGTATTCAAAGAGC
PRIMER_LEFT_17=1420,23
PRIMER_RIGHT_17=2026,23
PRIMER_LEFT_17_TM=59.881
PRIMER_RIGHT_17_TM=60.003
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER30 Nonautonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=8.4000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=607
PRIMER_PAIR_PENALTY_18=0.1226
PRIMER_LEFT_18_PENALTY=0.041227
PRIMER_RIGHT_18_PENALTY=0.081398
PRIMER_LEFT_18_SEQUENCE=ATGTGCCTGCTCTTTGAATACAT
PRIMER_RIGHT_18_SEQUENCE=TACTGGTGAAACTGGGTCTGTCT
PRIMER_LEFT_18=1996,23
PRIMER_RIGHT_18=2599,23
PRIMER_LEFT_18_TM=60.041
PRIMER_RIGHT_18_TM=60.081
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, reverse MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=5.6000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=604
PRIMER_PAIR_PENALTY_19=0.1267
PRIMER_LEFT_19_PENALTY=0.041227
PRIMER_RIGHT_19_PENALTY=0.085505
PRIMER_LEFT_19_SEQUENCE=ATGTGCCTGCTCTTTGAATACAT
PRIMER_RIGHT_19_SEQUENCE=TGAATACTGGTGAAACTGGGTCT
PRIMER_LEFT_19=1996,23
PRIMER_RIGHT_19=2603,23
PRIMER_LEFT_19_TM=60.041
PRIMER_RIGHT_19_TM=59.914
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1PB3 3'-end of L1 repeat (subfamily L1PB3) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse L1ME2 3'-end of L1 repeat (subfamily L1ME2) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.6000
PRIMER_RIGHT_19_END_STABILITY=7.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=608
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