PRIMER_SEQUENCE_ID=NM_005607
SEQUENCE=GCGCCGAGCCGCGGTCCGAAATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTTGAATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTATGGAACGTTCTCCTGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAACCACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGGGCATCATTCAGAAGATAGTGGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTCACCTGCGGTCAGAGGAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAAGTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATATGAATTGAGAATTCGTTATTTGCCAAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACAGGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAAGTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAAGAAGTCTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAGTTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACAATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGTCTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGTGGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCCCACACATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAAGGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGACGGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCTGGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTTGCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTCTGTGTCAGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCCCTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGAAGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGCGGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCAAGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCATCACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCTTAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCGGAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATATATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGCTCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGTGTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGATGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTACCCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTACTGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCGCATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGAAGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTATCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGACTCTACAGTATTGGACCTGCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGATGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGGAAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACCGCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGACAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAACCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCATCGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGAAGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACCAGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGAGCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTACAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGATGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCCTAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAGGAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCATCTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTGTTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTCTAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATTAAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAGCTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGAATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCCTTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACTTGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTGGATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTTTTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGATATTTCTCTTGGAAGCAGAAATGCTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTTCCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTGGAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAATTTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACCCTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACAGTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 47441, GC content failed 979, low tm 23397, high tm 12163, high any compl 4, high end compl 27, high repeat similarity 19, long poly-x seq 174, ok 10678
PRIMER_RIGHT_EXPLAIN=considered 47392, GC content failed 1031, low tm 23730, high tm 11925, high any compl 3, high end compl 4, high repeat similarity 13, long poly-x seq 185, ok 10501
PRIMER_PAIR_EXPLAIN=considered 983, unacceptable product size 920, high end compl 6, ok 57
PRIMER_PAIR_PENALTY=0.0074
PRIMER_LEFT_PENALTY=0.004430
PRIMER_RIGHT_PENALTY=0.002945
PRIMER_LEFT_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_SEQUENCE=AATGGATGGTGTAACTGGACATC
PRIMER_LEFT=3165,23
PRIMER_RIGHT=4015,23
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=851
PRIMER_PAIR_PENALTY_1=0.0074
PRIMER_LEFT_1_PENALTY=0.004430
PRIMER_RIGHT_1_PENALTY=0.002945
PRIMER_LEFT_1_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_1_SEQUENCE=GAATGGATGGTGTAACTGGACAT
PRIMER_LEFT_1=3165,23
PRIMER_RIGHT_1=4016,23
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.997
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=852
PRIMER_PAIR_PENALTY_2=0.0076
PRIMER_LEFT_2_PENALTY=0.004430
PRIMER_RIGHT_2_PENALTY=0.003218
PRIMER_LEFT_2_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_2_SEQUENCE=CAAATTCCTGTTTTGCTTCATTC
PRIMER_LEFT_2=3165,23
PRIMER_RIGHT_2=3982,23
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.997
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=818
PRIMER_PAIR_PENALTY_3=0.0171
PRIMER_LEFT_3_PENALTY=0.003045
PRIMER_RIGHT_3_PENALTY=0.014092
PRIMER_LEFT_3_SEQUENCE=TTGTCTAGAAATACGGCGATCAT
PRIMER_RIGHT_3_SEQUENCE=GATGATAAATGACTGCGAGGTTC
PRIMER_LEFT_3=566,23
PRIMER_RIGHT_3=1130,23
PRIMER_LEFT_3_TM=60.003
PRIMER_RIGHT_3_TM=59.986
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=565
PRIMER_PAIR_PENALTY_4=0.0349
PRIMER_LEFT_4_PENALTY=0.003045
PRIMER_RIGHT_4_PENALTY=0.031871
PRIMER_LEFT_4_SEQUENCE=TTGTCTAGAAATACGGCGATCAT
PRIMER_RIGHT_4_SEQUENCE=GAGTGAAGTCAGCAAGATGTGTG
PRIMER_LEFT_4=566,23
PRIMER_RIGHT_4=942,23
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=377
PRIMER_PAIR_PENALTY_5=0.0409
PRIMER_LEFT_5_PENALTY=0.004430
PRIMER_RIGHT_5_PENALTY=0.036444
PRIMER_LEFT_5_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_5_SEQUENCE=TTAGCATATTGCAACTGAAGGGT
PRIMER_LEFT_5=3165,23
PRIMER_RIGHT_5=3578,23
PRIMER_LEFT_5_TM=59.996
PRIMER_RIGHT_5_TM=60.036
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=7.3000
PRIMER_RIGHT_5_END_STABILITY=9.1000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=414
PRIMER_PAIR_PENALTY_6=0.0514
PRIMER_LEFT_6_PENALTY=0.048411
PRIMER_RIGHT_6_PENALTY=0.002945
PRIMER_LEFT_6_SEQUENCE=TTAGCTGCATAGTGGAAGAGGAG
PRIMER_RIGHT_6_SEQUENCE=GAATGGATGGTGTAACTGGACAT
PRIMER_LEFT_6=3436,23
PRIMER_RIGHT_6=4016,23
PRIMER_LEFT_6_TM=60.048
PRIMER_RIGHT_6_TM=59.997
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=3.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=581
PRIMER_PAIR_PENALTY_7=0.0514
PRIMER_LEFT_7_PENALTY=0.048411
PRIMER_RIGHT_7_PENALTY=0.002945
PRIMER_LEFT_7_SEQUENCE=CTTAGCTGCATAGTGGAAGAGGA
PRIMER_RIGHT_7_SEQUENCE=GAATGGATGGTGTAACTGGACAT
PRIMER_LEFT_7=3435,23
PRIMER_RIGHT_7=4016,23
PRIMER_LEFT_7_TM=60.048
PRIMER_RIGHT_7_TM=59.997
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=582
PRIMER_PAIR_PENALTY_8=0.0514
PRIMER_LEFT_8_PENALTY=0.048411
PRIMER_RIGHT_8_PENALTY=0.002945
PRIMER_LEFT_8_SEQUENCE=TTAGCTGCATAGTGGAAGAGGAG
PRIMER_RIGHT_8_SEQUENCE=AATGGATGGTGTAACTGGACATC
PRIMER_LEFT_8=3436,23
PRIMER_RIGHT_8=4015,23
PRIMER_LEFT_8_TM=60.048
PRIMER_RIGHT_8_TM=59.997
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.3000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=580
PRIMER_PAIR_PENALTY_9=0.0514
PRIMER_LEFT_9_PENALTY=0.048411
PRIMER_RIGHT_9_PENALTY=0.002945
PRIMER_LEFT_9_SEQUENCE=CTTAGCTGCATAGTGGAAGAGGA
PRIMER_RIGHT_9_SEQUENCE=AATGGATGGTGTAACTGGACATC
PRIMER_LEFT_9=3435,23
PRIMER_RIGHT_9=4015,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=59.997
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=6.3000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=581
PRIMER_PAIR_PENALTY_10=0.0516
PRIMER_LEFT_10_PENALTY=0.048411
PRIMER_RIGHT_10_PENALTY=0.003218
PRIMER_LEFT_10_SEQUENCE=CTGTGGATGCCAAAAACTTACTC
PRIMER_RIGHT_10_SEQUENCE=CAAATTCCTGTTTTGCTTCATTC
PRIMER_LEFT_10=2995,23
PRIMER_RIGHT_10=3982,23
PRIMER_LEFT_10_TM=60.048
PRIMER_RIGHT_10_TM=59.997
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=34.783
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=988
PRIMER_PAIR_PENALTY_11=0.0516
PRIMER_LEFT_11_PENALTY=0.048411
PRIMER_RIGHT_11_PENALTY=0.003218
PRIMER_LEFT_11_SEQUENCE=TTAGCTGCATAGTGGAAGAGGAG
PRIMER_RIGHT_11_SEQUENCE=CAAATTCCTGTTTTGCTTCATTC
PRIMER_LEFT_11=3436,23
PRIMER_RIGHT_11=3982,23
PRIMER_LEFT_11_TM=60.048
PRIMER_RIGHT_11_TM=59.997
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=6.9000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=547
PRIMER_PAIR_PENALTY_12=0.0516
PRIMER_LEFT_12_PENALTY=0.048411
PRIMER_RIGHT_12_PENALTY=0.003218
PRIMER_LEFT_12_SEQUENCE=CTTAGCTGCATAGTGGAAGAGGA
PRIMER_RIGHT_12_SEQUENCE=CAAATTCCTGTTTTGCTTCATTC
PRIMER_LEFT_12=3435,23
PRIMER_RIGHT_12=3982,23
PRIMER_LEFT_12_TM=60.048
PRIMER_RIGHT_12_TM=59.997
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=548
PRIMER_PAIR_PENALTY_13=0.0528
PRIMER_LEFT_13_PENALTY=0.004430
PRIMER_RIGHT_13_PENALTY=0.048411
PRIMER_LEFT_13_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_13_SEQUENCE=CTCCTCTTCCACTATGCAGCTAA
PRIMER_LEFT_13=3165,23
PRIMER_RIGHT_13=3458,23
PRIMER_LEFT_13_TM=59.996
PRIMER_RIGHT_13_TM=60.048
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=7.5000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=294
PRIMER_PAIR_PENALTY_14=0.0528
PRIMER_LEFT_14_PENALTY=0.004430
PRIMER_RIGHT_14_PENALTY=0.048411
PRIMER_LEFT_14_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_14_SEQUENCE=TCCTCTTCCACTATGCAGCTAAG
PRIMER_LEFT_14=3165,23
PRIMER_RIGHT_14=3457,23
PRIMER_LEFT_14_TM=59.996
PRIMER_RIGHT_14_TM=60.048
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER5b a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=6.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=293
PRIMER_PAIR_PENALTY_15=0.0528
PRIMER_LEFT_15_PENALTY=0.004430
PRIMER_RIGHT_15_PENALTY=0.048411
PRIMER_LEFT_15_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_15_SEQUENCE=ATTGCAACTGAAGGGTGTTCTAA
PRIMER_LEFT_15=3165,23
PRIMER_RIGHT_15=3571,23
PRIMER_LEFT_15_TM=59.996
PRIMER_RIGHT_15_TM=60.048
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=6.0000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=407
PRIMER_PAIR_PENALTY_16=0.0556
PRIMER_LEFT_16_PENALTY=0.052685
PRIMER_RIGHT_16_PENALTY=0.002945
PRIMER_LEFT_16_SEQUENCE=GCCACTGGAGTTAACTAACCCTT
PRIMER_RIGHT_16_SEQUENCE=AATGGATGGTGTAACTGGACATC
PRIMER_LEFT_16=3913,23
PRIMER_RIGHT_16=4015,23
PRIMER_LEFT_16_TM=59.947
PRIMER_RIGHT_16_TM=59.997
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=6.3000
PRIMER_PAIR_16_COMPL_ANY=7.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=103
PRIMER_PAIR_PENALTY_17=0.0571
PRIMER_LEFT_17_PENALTY=0.004430
PRIMER_RIGHT_17_PENALTY=0.052685
PRIMER_LEFT_17_SEQUENCE=CAGCACTTACAGCTCCAACTTTT
PRIMER_RIGHT_17_SEQUENCE=AAGGGTTAGTTAACTCCAGTGGC
PRIMER_LEFT_17=3165,23
PRIMER_RIGHT_17=3935,23
PRIMER_LEFT_17_TM=59.996
PRIMER_RIGHT_17_TM=59.947
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=9.4000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=771
PRIMER_PAIR_PENALTY_18=0.0724
PRIMER_LEFT_18_PENALTY=0.003045
PRIMER_RIGHT_18_PENALTY=0.069319
PRIMER_LEFT_18_SEQUENCE=TTGTCTAGAAATACGGCGATCAT
PRIMER_RIGHT_18_SEQUENCE=GGTGCACCTGCTATTTTTAGTTG
PRIMER_LEFT_18=566,23
PRIMER_RIGHT_18=1021,23
PRIMER_LEFT_18_TM=60.003
PRIMER_RIGHT_18_TM=60.069
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.5000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=7.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=456
PRIMER_PAIR_PENALTY_19=0.0849
PRIMER_LEFT_19_PENALTY=0.048411
PRIMER_RIGHT_19_PENALTY=0.036444
PRIMER_LEFT_19_SEQUENCE=TTAGCTGCATAGTGGAAGAGGAG
PRIMER_RIGHT_19_SEQUENCE=TTAGCATATTGCAACTGAAGGGT
PRIMER_LEFT_19=3436,23
PRIMER_RIGHT_19=3578,23
PRIMER_LEFT_19_TM=60.048
PRIMER_RIGHT_19_TM=60.036
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=9.1000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=143
=