PRIMER_SEQUENCE_ID=NM_005781
SEQUENCE=GAATTCCGGGAGCCCGCGGAGGCTGCTGCAGCAGCCCGAACCGGCGTCCGAGTCCGGGGAAGGCCCCCGCGAGCGCAGGGAGGGGCCGGAAAGTTCCGGCGAACTGGGGGAGCGGCAGTGGGAGAGCGTCGGGCGGGAGGCGGTGGTCCTGGGAGCGGCCCCGGCGCCCGAAGCCGCCCCGAGCTGGTGGGGAGGGTCCCGGCCGGTGGGGCCGGGCTGGCGGGGGAGGGGCGCCTGGGGGTGCAAGAGGCGAATTGGCTGCCCGGGGTGCAGGAGGGACAGGTGGTGCGGGCTGTCAGGAAACCTACCTGAACGGGAAGGAGCCCCAGGAGGGAGAGGGACCCGGCGAGGGCTCAGGACCCGGAGGCGCCGGCGGAGGAGGAGGTGGTGACTGGCAGGCCCGGGCCCCACGGTACCTCCGGGGCTGAAGGGGACGCAGGATGTAGGGGCATGGGGAGTCGGGCGCAGAAGAGTGCGGGGAACGCTGAGCTCTGGGAGCCACTGCCTGAGGGCAGGCCGCGGCCGGCGGGAACCTCTTCTGCCGTCTCAGCCTGGGCGTCGCTGAAGCTGTGTCTGCGGGGAGGCAGCGGAAGGCGGCAGAGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTGCAGCTGCAACAGTACTTCCTGCGGCTCCGAGATGACCTCAACGTCACCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCTGGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCTCAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTCACCTGCCTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTTGGCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGTGGCTGTGAAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGGGGTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGCTACGTAAGCACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCTGCCCGCAATCTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTACCTCAGAATGACGACCATTACGTCATGCAGGAACATCGCAAGGTGCCCTTCGCCTGGTGTGCCCCCGAGAGCCTGAAGACACGCACCTTCTCCCATGCCAGCGACACCTGGATGTTCGGGGTGACACTGTGGGAAATGTTCACCTACGGCCAGGAGCCCTGGATCGGCCTCAACGGCAGTCAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTGCCCCGGCCCGAGGACTGTCCCCAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGGACAGACCCACGTTTGTGGCCCTGCGGGACTTCCTGCTGGAGGCCCAGCCCACAGACATGCGGGCCCTTCAGGACTTTGAGGAACCGGACAAGCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAGGGAAGGGCCGAGAACTACTGGTGGCGTGGCCAGAACACACGGACGCTGTGTGTGGGGCCCTTCCCTCGCAACGTGGTGACCTCCGTGGCCGGCCTGTCGGCCCAGGACATCAGCCAGCCCCTGCAGAACAGCTTCATCCACACAGGGCATGGCGACAGTGACCCCCGCCACTGCTGGGGCTTCCCGGACAGGATTGACGAACTGTATCTGGGAAACCCCATGGACCCCCCCGACCTCCTGAGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGAAAAAACCAACCTATGACCCTGTGAGCGAGGACCAAGACCCCTTGTCCAGCGACTTCAAGAGGCTGGGCCTGCGGAAGCCAGGCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCGGCGCGGGTGCCGGGCACCAAGGCCAGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGGTGAGGAGCCCGTGGTCCCGGCCCTACGGCCCTGCCCGCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGCTCCCTGCTGGACGAGACCCCGCCTCAGAGCCCCACGCGGGCACTGCCCCGGCCCCTGCACCCCACGCCTGTGGTGGACTGGGACGCACGCCCGCTGCCCCCCCCGCCCGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGATCTGCTCCATCAACAGCACCCTCGTGGGCGCGGGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAACTACGCCTTTGTGCCTGAGCAGGCGCGGCCGCCCCCTCCCCTGGAGGACAACCTGTTCCTCCCGCCCCAGGGTGGGGGCAAGCCGCCCAGCTCCGCACAGACCGCAGAGATCTTCCAGGCGCTACAGCAGGAGTGCATGAGGCAACTGCAGGCTCCGGGCTCCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCCCAGGTGCCTCCTCGGGTACCCATCCCCCCTCGGCCCACGCGCCCACACGTCCAGCTGTCTCCAGCCCCCCCGGGCGAGGAGGAGACCAGCCAGTGGCCTGGACCTGCTTCCCCTCCCCGGGTGCCTCCGCGGGAGCCCCTGTCCCCTCAAGGCTCGAGGACACCCAGCCCCCTGGTACCACCTGGCAGCTCCCCGCTGCCACCCCGGCTCTCAAGCTCACCTGGGAAGACCATGCCCACCACCCAGAGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTGATCCAGGCCCCTGGCGCGGGTGGTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGAAGGTCAGCAGCACCCACTATTACTTGCTGCCCGAGCGACCATCCTACCTGGAGCGCTACCAGCGCTTCCTGCGTGAGGCCCAGAGCCCCGAGGAGCCTACCCCCCTGCCTGTGCCTCTGCTGCTGCCCCCACCCAGCACCCCAGCCCCCGCCGCCCCCACGGCCACCGTGCGGCCGATGCCCCAGGCTGCCTTGGACCCCAAGGCCAACTTCTCCACCAACAACAGCAACCCAGGGGCCCGGCCACCACCCCCGAGGGCCACTGCTCGGCTGCCACAGAGGGGCTGCCCTGGCGATGGGCCAGAGGCGGGCCGGCCAGCAGACAAGATCCAGATGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCTGCAGTGCCACGGCTGGAGCGTGCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTCGGGCTGGGTCTGCGGCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATGTTCGACTGGAACCTGGAGCAGGCCGGCTGCCACCTTCTGGGCTCCTGGGGCCCTGCCCACCACAAGCGCTGAGATGCGTCTGGAGAGCCAGAGGGCCTGCCTGAAGGAATCACCTGAGCCTGTCCGTCCACCAGGAGTGGGGAGATGCCCCCATCCAGTCCTGGAGGACCCGCTGCTCCTGCTGCTCCCGGGGATGGAGCAAGGCCAAGGCTGCGGGAGGCTGGGAGCCCTGCCCTGCCCATCCCTCCCGCACCAGTGCTGTCCCTGCACACTTTGGTTCAGTCCCGGTGCCCCTGCCAAGATGTGGAAGGGGCCGGGTGAAGACAGGCTTGAGGGCCGCCCCAGCAGGCTCTGGGTATGACCTGCCTCTGGCCCTGGTCCTGGGCGGGGCCTGTGGGTGGAGTAGTACCCCCAGGCCCTGCCCTGGGTGACAGACTGGGAGGAAACCAGGCTGGACCTGGGCAGGCGGGATGTGTTGGCCACAGGGAGAGGCGGACCGGCACCCGGTGGGACCTCCTAGGACTGGGCCTTCTTCCAGGGGGCCCCTGGCAGCAGCTGGGGTGTCGGGCAGAATGTGACTTGTGGCCTTACCATGGACTTGAATGGGACTTGGCTGGCCTCAGGATCTTGTGCCTGGAAATAGCCTGAGGTGGCTCAGGAAGCGGAGAAAGGGTGCCAGACCATTCTCTGGCGGGGACCAGGGCCCAAGGCCAGGGCTGGAAGGAGACCAAGGGGCAGCCCCTGGAGGACATCAGTGCTTCCTCTTCCACCCAATTCCCCCACGCGGTTCCATGTTTTCCCACCAGCCTGTTGGGCGAAGTTGCTGCTCCGGCATTCAGTCCTGCTTCTTCCAGAGAAATAAAGTTAGTTTCTATTTTATGTTAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 52173, GC content failed 6184, low tm 6764, high tm 31596, high any compl 25, high end compl 64, high repeat similarity 80, long poly-x seq 175, ok 7285
PRIMER_RIGHT_EXPLAIN=considered 52163, GC content failed 6071, low tm 7032, high tm 31343, high any compl 1, high end compl 4, high repeat similarity 18, long poly-x seq 174, ok 7520
PRIMER_PAIR_EXPLAIN=considered 1619, unacceptable product size 1559, high end compl 9, ok 51
PRIMER_PAIR_PENALTY=0.4185
PRIMER_LEFT_PENALTY=0.303373
PRIMER_RIGHT_PENALTY=0.115144
PRIMER_LEFT_SEQUENCE=AGGACATCTACAACGTCATGGTC
PRIMER_RIGHT_SEQUENCE=TTTCCCAGATACAGTTCGTCAAT
PRIMER_LEFT=1690,23
PRIMER_RIGHT=2083,23
PRIMER_LEFT_TM=60.303
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=394
PRIMER_PAIR_PENALTY_1=0.4192
PRIMER_LEFT_1_PENALTY=0.303373
PRIMER_RIGHT_1_PENALTY=0.115866
PRIMER_LEFT_1_SEQUENCE=AGGACATCTACAACGTCATGGTC
PRIMER_RIGHT_1_SEQUENCE=GATACAGTTCGTCAATCCTGTCC
PRIMER_LEFT_1=1690,23
PRIMER_RIGHT_1=2076,23
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=59.884
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=387
PRIMER_PAIR_PENALTY_2=0.5251
PRIMER_LEFT_2_PENALTY=0.115144
PRIMER_RIGHT_2_PENALTY=0.410006
PRIMER_LEFT_2_SEQUENCE=ATTGACGAACTGTATCTGGGAAA
PRIMER_RIGHT_2_SEQUENCE=ATCTCAAAGTCATCCTCATCCTG
PRIMER_LEFT_2=2061,23
PRIMER_RIGHT_2=2557,23
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=59.590
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MER21 a consensus
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=497
PRIMER_PAIR_PENALTY_3=0.5259
PRIMER_LEFT_3_PENALTY=0.115866
PRIMER_RIGHT_3_PENALTY=0.410006
PRIMER_LEFT_3_SEQUENCE=GGACAGGATTGACGAACTGTATC
PRIMER_RIGHT_3_SEQUENCE=ATCTCAAAGTCATCCTCATCCTG
PRIMER_LEFT_3=2054,23
PRIMER_RIGHT_3=2557,23
PRIMER_LEFT_3_TM=59.884
PRIMER_RIGHT_3_TM=59.590
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=504
PRIMER_PAIR_PENALTY_4=0.6698
PRIMER_LEFT_4_PENALTY=0.303373
PRIMER_RIGHT_4_PENALTY=0.366457
PRIMER_LEFT_4_SEQUENCE=AGGACATCTACAACGTCATGGTC
PRIMER_RIGHT_4_SEQUENCE=TTCCCAGATACAGTTCGTCAATC
PRIMER_LEFT_4=1690,23
PRIMER_RIGHT_4=2082,23
PRIMER_LEFT_4_TM=60.303
PRIMER_RIGHT_4_TM=60.366
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=393
PRIMER_PAIR_PENALTY_5=0.7125
PRIMER_LEFT_5_PENALTY=0.409123
PRIMER_RIGHT_5_PENALTY=0.303373
PRIMER_LEFT_5_SEQUENCE=GGATGAGTAAGGTGTTCAGTGGA
PRIMER_RIGHT_5_SEQUENCE=GACCATGACGTTGTAGATGTCCT
PRIMER_LEFT_5=841,23
PRIMER_RIGHT_5=1712,23
PRIMER_LEFT_5_TM=60.409
PRIMER_RIGHT_5_TM=60.303
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=7.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=872
PRIMER_PAIR_PENALTY_6=0.7134
PRIMER_LEFT_6_PENALTY=0.303373
PRIMER_RIGHT_6_PENALTY=0.410006
PRIMER_LEFT_6_SEQUENCE=AGGACATCTACAACGTCATGGTC
PRIMER_RIGHT_6_SEQUENCE=ATCTCAAAGTCATCCTCATCCTG
PRIMER_LEFT_6=1690,23
PRIMER_RIGHT_6=2557,23
PRIMER_LEFT_6_TM=60.303
PRIMER_RIGHT_6_TM=59.590
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=868
PRIMER_PAIR_PENALTY_7=0.7187
PRIMER_LEFT_7_PENALTY=0.309542
PRIMER_RIGHT_7_PENALTY=0.409123
PRIMER_LEFT_7_SEQUENCE=AGTACGTCAAGAATGAGGACCTG
PRIMER_RIGHT_7_SEQUENCE=TCCACTGAACACCTTACTCATCC
PRIMER_LEFT_7=733,23
PRIMER_RIGHT_7=863,23
PRIMER_LEFT_7_TM=59.690
PRIMER_RIGHT_7_TM=60.409
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=8.1000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=131
PRIMER_PAIR_PENALTY_8=0.7427
PRIMER_LEFT_8_PENALTY=0.439285
PRIMER_RIGHT_8_PENALTY=0.303373
PRIMER_LEFT_8_SEQUENCE=AGTCGTGGATGAGTAAGGTGTTC
PRIMER_RIGHT_8_SEQUENCE=GACCATGACGTTGTAGATGTCCT
PRIMER_LEFT_8=835,23
PRIMER_RIGHT_8=1712,23
PRIMER_LEFT_8_TM=59.561
PRIMER_RIGHT_8_TM=60.303
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=878
PRIMER_PAIR_PENALTY_9=0.7488
PRIMER_LEFT_9_PENALTY=0.309542
PRIMER_RIGHT_9_PENALTY=0.439285
PRIMER_LEFT_9_SEQUENCE=AGTACGTCAAGAATGAGGACCTG
PRIMER_RIGHT_9_SEQUENCE=GAACACCTTACTCATCCACGACT
PRIMER_LEFT_9=733,23
PRIMER_RIGHT_9=857,23
PRIMER_LEFT_9_TM=59.690
PRIMER_RIGHT_9_TM=59.561
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=125
PRIMER_PAIR_PENALTY_10=0.7654
PRIMER_LEFT_10_PENALTY=0.462030
PRIMER_RIGHT_10_PENALTY=0.303373
PRIMER_LEFT_10_SEQUENCE=CGTGGATGAGTAAGGTGTTCAGT
PRIMER_RIGHT_10_SEQUENCE=GACCATGACGTTGTAGATGTCCT
PRIMER_LEFT_10=838,23
PRIMER_RIGHT_10=1712,23
PRIMER_LEFT_10_TM=60.462
PRIMER_RIGHT_10_TM=60.303
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.6000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=875
PRIMER_PAIR_PENALTY_11=0.7654
PRIMER_LEFT_11_PENALTY=0.462030
PRIMER_RIGHT_11_PENALTY=0.303373
PRIMER_LEFT_11_SEQUENCE=CAAGTCGTGGATGAGTAAGGTGT
PRIMER_RIGHT_11_SEQUENCE=GACCATGACGTTGTAGATGTCCT
PRIMER_LEFT_11=833,23
PRIMER_RIGHT_11=1712,23
PRIMER_LEFT_11_TM=60.462
PRIMER_RIGHT_11_TM=60.303
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.6000
PRIMER_RIGHT_11_END_STABILITY=7.6000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=880
PRIMER_PAIR_PENALTY_12=0.7716
PRIMER_LEFT_12_PENALTY=0.309542
PRIMER_RIGHT_12_PENALTY=0.462030
PRIMER_LEFT_12_SEQUENCE=AGTACGTCAAGAATGAGGACCTG
PRIMER_RIGHT_12_SEQUENCE=ACACCTTACTCATCCACGACTTG
PRIMER_LEFT_12=733,23
PRIMER_RIGHT_12=855,23
PRIMER_LEFT_12_TM=59.690
PRIMER_RIGHT_12_TM=60.462
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=123
PRIMER_PAIR_PENALTY_13=0.7765
PRIMER_LEFT_13_PENALTY=0.366457
PRIMER_RIGHT_13_PENALTY=0.410006
PRIMER_LEFT_13_SEQUENCE=GATTGACGAACTGTATCTGGGAA
PRIMER_RIGHT_13_SEQUENCE=ATCTCAAAGTCATCCTCATCCTG
PRIMER_LEFT_13=2060,23
PRIMER_RIGHT_13=2557,23
PRIMER_LEFT_13_TM=60.366
PRIMER_RIGHT_13_TM=59.590
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=498
PRIMER_PAIR_PENALTY_14=0.8009
PRIMER_LEFT_14_PENALTY=0.685716
PRIMER_RIGHT_14_PENALTY=0.115144
PRIMER_LEFT_14_SEQUENCE=ACACTGTGGGAAATGTTCACCTA
PRIMER_RIGHT_14_SEQUENCE=TTTCCCAGATACAGTTCGTCAAT
PRIMER_LEFT_14=1572,23
PRIMER_RIGHT_14=2083,23
PRIMER_LEFT_14_TM=60.686
PRIMER_RIGHT_14_TM=59.885
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_LEFT_14_END_STABILITY=6.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=7.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=512
PRIMER_PAIR_PENALTY_15=0.8016
PRIMER_LEFT_15_PENALTY=0.685716
PRIMER_RIGHT_15_PENALTY=0.115866
PRIMER_LEFT_15_SEQUENCE=ACACTGTGGGAAATGTTCACCTA
PRIMER_RIGHT_15_SEQUENCE=GATACAGTTCGTCAATCCTGTCC
PRIMER_LEFT_15=1572,23
PRIMER_RIGHT_15=2076,23
PRIMER_LEFT_15_TM=60.686
PRIMER_RIGHT_15_TM=59.884
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=505
PRIMER_PAIR_PENALTY_16=0.8890
PRIMER_LEFT_16_PENALTY=0.309542
PRIMER_RIGHT_16_PENALTY=0.579444
PRIMER_LEFT_16_SEQUENCE=AGTACGTCAAGAATGAGGACCTG
PRIMER_RIGHT_16_SEQUENCE=GAAGGTGCTCTGAGAGTGATGAG
PRIMER_LEFT_16=733,23
PRIMER_RIGHT_16=911,23
PRIMER_LEFT_16_TM=59.690
PRIMER_RIGHT_16_TM=60.579
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=179
PRIMER_PAIR_PENALTY_17=0.9891
PRIMER_LEFT_17_PENALTY=0.685716
PRIMER_RIGHT_17_PENALTY=0.303373
PRIMER_LEFT_17_SEQUENCE=ACACTGTGGGAAATGTTCACCTA
PRIMER_RIGHT_17_SEQUENCE=GACCATGACGTTGTAGATGTCCT
PRIMER_LEFT_17=1572,23
PRIMER_RIGHT_17=1712,23
PRIMER_LEFT_17_TM=60.686
PRIMER_RIGHT_17_TM=60.303
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.9000
PRIMER_RIGHT_17_END_STABILITY=7.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=141
PRIMER_PAIR_PENALTY_18=1.0522
PRIMER_LEFT_18_PENALTY=0.685716
PRIMER_RIGHT_18_PENALTY=0.366457
PRIMER_LEFT_18_SEQUENCE=ACACTGTGGGAAATGTTCACCTA
PRIMER_RIGHT_18_SEQUENCE=TTCCCAGATACAGTTCGTCAATC
PRIMER_LEFT_18=1572,23
PRIMER_RIGHT_18=2082,23
PRIMER_LEFT_18_TM=60.686
PRIMER_RIGHT_18_TM=60.366
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MER21 a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=6.9000
PRIMER_PAIR_18_COMPL_ANY=6.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=511
PRIMER_PAIR_PENALTY_19=1.0957
PRIMER_LEFT_19_PENALTY=0.685716
PRIMER_RIGHT_19_PENALTY=0.410006
PRIMER_LEFT_19_SEQUENCE=ACACTGTGGGAAATGTTCACCTA
PRIMER_RIGHT_19_SEQUENCE=ATCTCAAAGTCATCCTCATCCTG
PRIMER_LEFT_19=1572,23
PRIMER_RIGHT_19=2557,23
PRIMER_LEFT_19_TM=60.686
PRIMER_RIGHT_19_TM=59.590
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=986
=