PRIMER_SEQUENCE_ID=NM_005975
SEQUENCE=CCATGGGCGCTTCCAGGCCGCACTCACGGTCCTTGCCTGCCCCCTGGCGTCCGTGTGGAGGAAGGCAGTGCCCGTTTCTTCAGCCGTGGGTGCCCCTGGCTCTGCCTCCCCGGGGATCCCCCAAAACCCTCGGGCCCTCCTCTTCCGGGAAGTCCTCCGGGCCGACCCACACAGCTGTCGCTGCCCTGATTCCACTCCCTCTTCCCTGCTTGGCCCTTACCCGAGGGCGGGCCTGCCCATGAGGCCGCTCTCAGGACGCCTGCCCACAGCAGCTCCTGACATTGGGCCCTGTGCTCAGGGGTGGAGTCTGGGGCTGGGTGAGGGCACCGACGGGTGGAGGGGAAGCTGCCGGAAGCGGGGAGAGAGGCGGCCCCCACCTGTGCCCCCGCAGCTCTGAGGGTTGGGAAGGCAGCGCCTGCAGCCCTCACCTTCCTGCCCTCAGGCTGCGAGGCTTGTTCCCAGAGGGCCGTTGGCTTCCCTGAGTGCCTGAGTGTCCATAAGTTGGACCCCCAAGAAGCCCCAACCGGGAGGTCCTCCTCCCCCTCTCCTGCGCTCAGGCCGGCCCTGGCGGTGCTTGCCCAGTGGGTTCCCATAGTGCGGGCGCTAAGTGCTCAGATGGGCCCCAGTGGGCCTGAGGACTTGTGGGGCCGGCTGGGCCCTCAGGAGGAGCCGGGGTGCTCTGGAGCGCCTGTGGGTGCTGGGTGTGGCTGCTTCCCGGCCTGTCATGAGGAAGTGGGACGGCCCGCCTGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTGGTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGGTTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTGCCGACTACGTCCTGTCGGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGCCGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAGTTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGGAAAGACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAGATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTGTACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATGGCCAAGGGCAGCCTGCTGGAGCTGCTCCGCGACTCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTGGACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCACCGGGACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGACTTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTCCCATGACCACAATATCCCCTACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAAATCCGACGTCTGGTCCTTTGGGATTCTCCTGCATGAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGCATGTCCAACCATGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTGGAGTGCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAGAGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCACCAGCTACGAGAACCCGACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAGGGGCCTGGGCAGAGGGCCTGGACCTGGGATCAAGGCCCACGCGCTTCCCTGGGGTTTACTGAGGTGATGGGTGCAGGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTACTCCATCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGATGAGATCCGGGAGACTGACACGAAGCCAGCAGAGGTCAGAGGGGACTCTGACCACAGCCCGCTCTCTGGCTGTCTGTCTGCAGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCCTGCTCTTCCCAGTTCAGCTTGGTGGGAGAAAGTCATTCGCGTGGCTCGGGACGCTCATGTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAGGGGCAGGTGTTTCTTTCCTGTTTGTCTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCTCCCTGGTCTCTGCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACCGTCCACGTCCACAAGGGGCTTTTCCCACCTTCACCTTTGTCGCTGGGTCAGTGCTGGAAAGCGCCCCTCACTCCTGCGCTGACAAGGGCCCTTCTCTACTGTCTGTGGGGTGGTTCCGGGCTGGGGGGGCTGCCTCCTTTGCACCTGATTTTGAAGGTGTCTCTTTCATCCATGGTTAAGTCATAAAAAGCTTATTGGTTTTGGTTTTGACTCACCTGAAAGTTTTTTTGGTTTAAAAGAAGAATAGGCGGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCACGAGGTCAGGAGATCGACACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAAATACAAAAAATTAGCTGGGTGTGGTGGTGGGGGTGGGCGCCTGTGATCCCAGCTACGTGGGAGGCTGAGGCAGCAGACTGGTGTGAACCCGGGAGGTGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAGAAAGAAAGAAAAATAATTTTGGGAGTTTCTGGAAAGGTACTAGGATTTCTCAAAAGGATTTGTCTTCTCCCTTGTGAAAGACAGATGTCAGACTAATCAGGCTTATCCGATGTGCTACATGAGATGGAAATGCGTGTGAAATAGTAAGTCACACTAAGTCTTCTGGAGGTTCTATTTACGGGTTTGGTTTGATATGAATGTTGCAGAAACTCTATAAAATTCTAGA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 40565, GC content failed 2032, low tm 7399, high tm 24119, high any compl 3, high end compl 36, high repeat similarity 53, long poly-x seq 83, ok 6840
PRIMER_RIGHT_EXPLAIN=considered 40541, GC content failed 1989, low tm 8070, high tm 23421, high end compl 7, high repeat similarity 3, long poly-x seq 89, ok 6962
PRIMER_PAIR_EXPLAIN=considered 526, unacceptable product size 444, high any compl 3, high end compl 8, ok 71
PRIMER_PAIR_PENALTY=0.0824
PRIMER_LEFT_PENALTY=0.034654
PRIMER_RIGHT_PENALTY=0.047725
PRIMER_LEFT_SEQUENCE=TACCTGGAGTCGCAGAATTACAT
PRIMER_RIGHT_SEQUENCE=AGACACTCCACATTTGTGAACCT
PRIMER_LEFT=1716,23
PRIMER_RIGHT=2309,23
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.952
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=594
PRIMER_PAIR_PENALTY_1=0.0957
PRIMER_LEFT_1_PENALTY=0.034654
PRIMER_RIGHT_1_PENALTY=0.061028
PRIMER_LEFT_1_SEQUENCE=TACCTGGAGTCGCAGAATTACAT
PRIMER_RIGHT_1_SEQUENCE=GACAAACAGGAAAGAAACACCTG
PRIMER_LEFT_1=1716,23
PRIMER_RIGHT_1=2647,23
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=932
PRIMER_PAIR_PENALTY_2=0.1088
PRIMER_LEFT_2_PENALTY=0.047725
PRIMER_RIGHT_2_PENALTY=0.061028
PRIMER_LEFT_2_SEQUENCE=AGGTTCACAAATGTGGAGTGTCT
PRIMER_RIGHT_2_SEQUENCE=GACAAACAGGAAAGAAACACCTG
PRIMER_LEFT_2=2287,23
PRIMER_RIGHT_2=2647,23
PRIMER_LEFT_2_TM=59.952
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=361
PRIMER_PAIR_PENALTY_3=0.1261
PRIMER_LEFT_3_PENALTY=0.008181
PRIMER_RIGHT_3_PENALTY=0.117879
PRIMER_LEFT_3_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_3_SEQUENCE=CTGCAACATTCATATCAAACCAA
PRIMER_LEFT_3=3352,23
PRIMER_RIGHT_3=3506,23
PRIMER_LEFT_3_TM=59.992
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=34.783
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.1275
PRIMER_LEFT_4_PENALTY=0.009608
PRIMER_RIGHT_4_PENALTY=0.117879
PRIMER_LEFT_4_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_4_SEQUENCE=CTGCAACATTCATATCAAACCAA
PRIMER_LEFT_4=2896,23
PRIMER_RIGHT_4=3506,23
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.882
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=611
PRIMER_PAIR_PENALTY_5=0.1697
PRIMER_LEFT_5_PENALTY=0.009608
PRIMER_RIGHT_5_PENALTY=0.160134
PRIMER_LEFT_5_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_5_SEQUENCE=GACATCTGTCTTTCACAAGGGAG
PRIMER_LEFT_5=2896,23
PRIMER_RIGHT_5=3389,23
PRIMER_LEFT_5_TM=59.990
PRIMER_RIGHT_5_TM=60.160
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=494
PRIMER_PAIR_PENALTY_6=0.1783
PRIMER_LEFT_6_PENALTY=0.034654
PRIMER_RIGHT_6_PENALTY=0.143622
PRIMER_LEFT_6_SEQUENCE=TACCTGGAGTCGCAGAATTACAT
PRIMER_RIGHT_6_SEQUENCE=GAGAGGTAGACGTCCTCCTTGAT
PRIMER_LEFT_6=1716,23
PRIMER_RIGHT_6=1843,23
PRIMER_LEFT_6_TM=60.035
PRIMER_RIGHT_6_TM=60.144
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=52.174
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=6.9000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=128
PRIMER_PAIR_PENALTY_7=0.2105
PRIMER_LEFT_7_PENALTY=0.034654
PRIMER_RIGHT_7_PENALTY=0.175797
PRIMER_LEFT_7_SEQUENCE=TACCTGGAGTCGCAGAATTACAT
PRIMER_RIGHT_7_SEQUENCE=ACACTCCACATTTGTGAACCTTT
PRIMER_LEFT_7=1716,23
PRIMER_RIGHT_7=2307,23
PRIMER_LEFT_7_TM=60.035
PRIMER_RIGHT_7_TM=59.824
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1MB3 3'-end of L1 repeat (subfamily L1MB3) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=8.5000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=592
PRIMER_PAIR_PENALTY_8=0.2148
PRIMER_LEFT_8_PENALTY=0.008181
PRIMER_RIGHT_8_PENALTY=0.206636
PRIMER_LEFT_8_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_8_SEQUENCE=AAACCCGTAAATAGAACCTCCAG
PRIMER_LEFT_8=3352,23
PRIMER_RIGHT_8=3485,23
PRIMER_LEFT_8_TM=59.992
PRIMER_RIGHT_8_TM=59.793
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=134
PRIMER_PAIR_PENALTY_9=0.2162
PRIMER_LEFT_9_PENALTY=0.009608
PRIMER_RIGHT_9_PENALTY=0.206636
PRIMER_LEFT_9_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_9_SEQUENCE=AAACCCGTAAATAGAACCTCCAG
PRIMER_LEFT_9=2896,23
PRIMER_RIGHT_9=3485,23
PRIMER_LEFT_9_TM=59.990
PRIMER_RIGHT_9_TM=59.793
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.1000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=8.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=590
PRIMER_PAIR_PENALTY_10=0.2286
PRIMER_LEFT_10_PENALTY=0.009608
PRIMER_RIGHT_10_PENALTY=0.219014
PRIMER_LEFT_10_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_10_SEQUENCE=GTCTTTCACAAGGGAGAAGACAA
PRIMER_LEFT_10=2896,23
PRIMER_RIGHT_10=3382,23
PRIMER_LEFT_10_TM=59.990
PRIMER_RIGHT_10_TM=59.781
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=8.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=487
PRIMER_PAIR_PENALTY_11=0.2368
PRIMER_LEFT_11_PENALTY=0.175797
PRIMER_RIGHT_11_PENALTY=0.061028
PRIMER_LEFT_11_SEQUENCE=AAAGGTTCACAAATGTGGAGTGT
PRIMER_RIGHT_11_SEQUENCE=GACAAACAGGAAAGAAACACCTG
PRIMER_LEFT_11=2285,23
PRIMER_RIGHT_11=2647,23
PRIMER_LEFT_11_TM=59.824
PRIMER_RIGHT_11_TM=60.061
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER9 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=363
PRIMER_PAIR_PENALTY_12=0.2383
PRIMER_LEFT_12_PENALTY=0.008181
PRIMER_RIGHT_12_PENALTY=0.230075
PRIMER_LEFT_12_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_12_SEQUENCE=TCAAACCAAACCCGTAAATAGAA
PRIMER_LEFT_12=3352,23
PRIMER_RIGHT_12=3492,23
PRIMER_LEFT_12_TM=59.992
PRIMER_RIGHT_12_TM=59.770
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=6.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=141
PRIMER_PAIR_PENALTY_13=0.2397
PRIMER_LEFT_13_PENALTY=0.009608
PRIMER_RIGHT_13_PENALTY=0.230075
PRIMER_LEFT_13_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_13_SEQUENCE=TCAAACCAAACCCGTAAATAGAA
PRIMER_LEFT_13=2896,23
PRIMER_RIGHT_13=3492,23
PRIMER_LEFT_13_TM=59.990
PRIMER_RIGHT_13_TM=59.770
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=34.783
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MER21B a consensus
PRIMER_LEFT_13_END_STABILITY=8.1000
PRIMER_RIGHT_13_END_STABILITY=6.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=597
PRIMER_PAIR_PENALTY_14=0.2404
PRIMER_LEFT_14_PENALTY=0.008181
PRIMER_RIGHT_14_PENALTY=0.232197
PRIMER_LEFT_14_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_14_SEQUENCE=TGCAACATTCATATCAAACCAAA
PRIMER_LEFT_14=3352,23
PRIMER_RIGHT_14=3505,23
PRIMER_LEFT_14_TM=59.992
PRIMER_RIGHT_14_TM=60.232
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=30.435
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=8.8000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=154
PRIMER_PAIR_PENALTY_15=0.2418
PRIMER_LEFT_15_PENALTY=0.009608
PRIMER_RIGHT_15_PENALTY=0.232197
PRIMER_LEFT_15_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_15_SEQUENCE=TGCAACATTCATATCAAACCAAA
PRIMER_LEFT_15=2896,23
PRIMER_RIGHT_15=3505,23
PRIMER_LEFT_15_TM=59.990
PRIMER_RIGHT_15_TM=60.232
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=30.435
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=8.8000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=610
PRIMER_PAIR_PENALTY_16=0.2446
PRIMER_LEFT_16_PENALTY=0.008181
PRIMER_RIGHT_16_PENALTY=0.236447
PRIMER_LEFT_16_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_16_SEQUENCE=ACCCGTAAATAGAACCTCCAGAA
PRIMER_LEFT_16=3352,23
PRIMER_RIGHT_16=3483,23
PRIMER_LEFT_16_TM=59.992
PRIMER_RIGHT_16_TM=60.236
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_16_END_STABILITY=8.5000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=132
PRIMER_PAIR_PENALTY_17=0.2446
PRIMER_LEFT_17_PENALTY=0.008181
PRIMER_RIGHT_17_PENALTY=0.236447
PRIMER_LEFT_17_SEQUENCE=AAAAGGATTTGTCTTCTCCCTTG
PRIMER_RIGHT_17_SEQUENCE=AACCCGTAAATAGAACCTCCAGA
PRIMER_LEFT_17=3352,23
PRIMER_RIGHT_17=3484,23
PRIMER_LEFT_17_TM=59.992
PRIMER_RIGHT_17_TM=60.236
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=8.5000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=133
PRIMER_PAIR_PENALTY_18=0.2461
PRIMER_LEFT_18_PENALTY=0.009608
PRIMER_RIGHT_18_PENALTY=0.236447
PRIMER_LEFT_18_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_18_SEQUENCE=ACCCGTAAATAGAACCTCCAGAA
PRIMER_LEFT_18=2896,23
PRIMER_RIGHT_18=3483,23
PRIMER_LEFT_18_TM=59.990
PRIMER_RIGHT_18_TM=60.236
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT1d (MLT1d subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=8.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=588
PRIMER_PAIR_PENALTY_19=0.2461
PRIMER_LEFT_19_PENALTY=0.009608
PRIMER_RIGHT_19_PENALTY=0.236447
PRIMER_LEFT_19_SEQUENCE=TTGAAGGTGTCTCTTTCATCCAT
PRIMER_RIGHT_19_SEQUENCE=AACCCGTAAATAGAACCTCCAGA
PRIMER_LEFT_19=2896,23
PRIMER_RIGHT_19=3484,23
PRIMER_LEFT_19_TM=59.990
PRIMER_RIGHT_19_TM=60.236
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=8.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=589
=