PRIMER_SEQUENCE_ID=NM_006180
SEQUENCE=GGGAGCAGGAGCCTCGCTGGCTGCTTCGCTCGCGCTCTACGCGCTCAGTCCCCGGCGGTAGCAGGAGCCTGGACCCAGGCGCCGGCGGCGGGCGTGAGGCGCCGGAGCCCGGCCTCGAGGTGCATACCGGACCCCCATTCGCATCTAACAAGGAATCTGCGCCCCAGAGAGTCCCGGACGCCGCCGGTCGGTGCCCGGCGCGCCGGGCCATGCAGCGACGGCCGCCGCGGAGCTCCGAGCAGCGGTAGCGCCCCCCTGTAAAGCGGTTCGCTATGCCGGGACCACTGTGAACCCTGCCGCCTGCCGGAACACTCTTCGCTCCGGACCAGCTCAGCCTCTGATAAGCTGGACTCGGCACGCCCGCAACAAGCACCGAGGAGTTAAGAGAGCCGCAAGCGCAGGGAAGGCCTCCCCGCACGGGTGGGGGAAAGCGGCCGGTGCAGCGCGGGGACAGGCACTCGGGCTGGCACTGGCTGCTAGGGATGTCGTCCTGGATAAGGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTCTGCTGGCTGGTTGTGGGCTTCTGGAGGGCCGCTTTCGCCTGTCCCACGTCCTGCAAATGCAGTGCCTCTCGGATCTGGTGCAGCGACCCTTCTCCTGGCATCGTGGCATTTCCGAGATTGGAGCCTAACAGTGTAGATCCTGAGAACATCACCGAAATTTTCATCGCAAACCAGAAAAGGTTAGAAATCATCAACGAAGATGATGTTGAAGCTTATGTGGGACTGAGAAATCTGACAATTGTGGATTCTGGATTAAAATTTGTGGCTCATAAAGCATTTCTGAAAAACAGCAACCTGCAGCACATCAATTTTACCCGAAACAAACTGACGAGTTTGTCTAGGAAACATTTCCGTCACCTTGACTTGTCTGAACTGATCCTGGTGGGCAATCCATTTACATGCTCCTGTGACATTATGTGGATCAAGACTCTCCAAGAGGCTAAATCCAGTCCAGACACTCAGGATTTGTACTGCCTGAATGAAAGCAGCAAGAATATTCCCCTGGCAAACCTGCAGATACCCAATTGTGGTTTGCCATCTGCAAATCTGGCCGCACCTAACCTCACTGTGGAGGAAGGAAAGTCTATCACATTATCCTGTAGTGTGGCAGGTGATCCGGTTCCTAATATGTATTGGGATGTTGGTAACCTGGTTTCCAAACATATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACTAACATTTCATCCGATGACAGTGGGAAGCAGATCTCTTGTGTGGCGGAAAATCTTGTAGGAGAAGATCAAGATTCTGTCAACCTCACTGTGCATTTTGCACCAACTATCACATTTCTCGAATCTCCAACCTCAGACCACCACTGGTGCATTCCATTCACTGTGAAAGGCAACCCCAAACCAGCGCTTCAGTGGTTCTATAACGGGGCAATATTGAATGAGTCCAAATACATCTGTACTAAAATACATGTTACCAATCACACGGAGTACCACGGCTGCCTCCAGCTGGATAATCCCACTCACATGAACAATGGGGACTACACTCTAATAGCCAAGAATGAGTATGGGAAGGATGAGAAACAGATTTCTGCTCACTTCATGGGCTGGCCTGGAATTGACGATGGTGCAAACCCAAATTATCCTGATGTAATTTATGAAGATTATGGAACTGCAGCGAATGACATCGGGGACACCACGAACAGAAGTAATGAAATCCCTTCCACAGACGTCACTGATAAAACCGGTCGGGAACATCTCTCGGTCTATGCTGTGGTGGTGATTGCGTCTGTGGTGGGATTTTGCCTTTTGGTAATGCTGTTTCTGCTTAAGTTGGCAAGACACTCCAAGTTTGGCATGAAAGATTTCTCATGGTTTGGATTTGGGAAAGTAAAATCAAGACAAGGTGTTGGCCCAGCCTCCGTTATCAGCAATGATGATGACTCTGCCAGCCCACTCCATCACATCTCCAATGGGAGTAACACTCCATCTTCTTCGGAAGGTGGCCCAGATGCTGTCATTATTGGAATGACCAAGATCCCTGTCATTGAAAATCCCCAGTACTTTGGCATCACCAACAGTCAGCTCAAGCCAGACACATTTGTTCAGCACATCAAGCGACATAACATTGTTCTGAAAAGGGAGCTAGGCGAAGGAGCCTTTGGAAAAGTGTTCCTAGCTGAATGCTATAACCTCTGTCCTGAGCAGGACAAGATCTTGGTGGCAGTGAAGACCCTGAAGGATGCCAGTGACAATGCACGCAAGGACTTCCACCGTGAGGCCGAGCTCCTGACCAACCTCCAGCATGAGCACATCGTCAAGTTCTATGGCGTCTGCGTGGAGGGCGACCCCCTCATCATGGTCTTTGAGTACATGAAGCATGGGGACCTCAACAAGTTCCTCAGGGCACACGGCCCTGATGCCGTGCTGATGGCTGAGGGCAACCCGCCCACGGAACTGACGCAGTCGCAGATGCTGCATATAGCCCAGCAGATCGCCGCGGGCATGGTCTACCTGGCGTCCCAGCACTTCGTGCACCGCGATTTGGCCACCAGGAACTGCCTGGTCGGGGAGAACTTGCTGGTGAAAATCGGGGACTTTGGGATGTCCCGGGACGTGTACAGCACTGACTACTACAGGGTCGGTGGCCACACAATGCTGCCCATTCGCTGGATGCCTCCAGAGAGCATCATGTACAGGAAATTCACGACGGAAAGCGACGTCTGGAGCCTGGGGGTCGTGTTGTGGGAGATTTTCACCTATGGCAAACAGCCCTGGTACCAGCTGTCAAACAATGAGGTGATAGAGTGTATCACTCAGGGCCGAGTCCTGCAGCGACCCCGCACGTGCCCCCAGGAGGTGTATGAGCTGATGCTGGGGTGCTGGCAGCGAGAGCCCCACATGAGGAAGAACATCAAGGGCATCCATACCCTCCTTCAGAACTTGGCCAAGGCATCTCCGGTCTACCTGGACATTCTAGGCTAGGGCCCTTTTCCCCAGACCGATCCTTCCCAACGTACTCCTCAGACGGGCTGAGAGGATGAACATCTTTTAACTGCCGCTGGAGGCCACCAAGCTGCTCTCCTTCACTCTGACAGTATTAACATCAAAGACTCCGAGAAGCTCTCGAGGGAAGCAGTGTGTACTTCTTCATCCATAGACACAGTATTGACTTCTTTTTGGCATTATCTCTTTCTCTCTTTCCATCTCCCTTGGTTGTTCCTTTTTCTTTTTTTAAATTTTCTTTTTCTTCTTTTTTTTCGTCTTCCCTGCTTCACGATTCTTACCCTTTCTTTTGAATCAATCTGGCTTCTGCATTACTATTAACTCTGCATAGACAAAGGCCTTAACAAACGTAATTTGTTATATCAGCAGACACTCCAGTTTGCCCACCACAACTAACAATGCCTTGTTGTATTCCTGCCTTTGATGTGGATGAAAAAAAGGGAAAACAAATATTTCACTTAAACTTTGTCACTTCTGCTGTACAGATATCGAGAGTTTCTATGGATTCACTTCTATTTATTTATTATTATTACTGTTCTTATTGTTTTTGGATGGCTTAAGCCTGTGTATAAAAAAGAAAACTTGTGTTCAATCTGTGAAGCCTTTATCTATGGGAGATTAAAACCAGAGAGAAAGAAGATTTATTATGAACCGCAATATGGGAGGAACAAAGACAACCACTGGGATCAGCTGGTGTCAGTCCCTACTTAGGAAATACTCAGCAACTGTTAGCTGGGAAGAATGTATTCGGCACCTTCCCCTGAGGACCTTTCTGAGGAGTAAAAAGACTACTGGCCTCTGTGCCATGGATGATTCTTTTCCCATCACCAGAAATGATAGCGTGCAGTAGAGAGCAAAGATGGCTTCCGTGAGACACAAGATGGCGCATAGTGTGCTCGGACACAGTTTTGTCTTCGTAGGTTGTGATGATAGCACTGGTTTGTTTCTCAAGCGCTATCCACAGAACCTTTGTCAACTTCAGTTGAAAAGAGGTGGATTCATGTCCAGAGCTCATTTCGGGGTCAGGTGGGAAAGCC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 46829, GC content failed 1982, low tm 17845, high tm 17039, high any compl 7, high end compl 30, high repeat similarity 44, long poly-x seq 90, ok 9792
PRIMER_RIGHT_EXPLAIN=considered 46806, GC content failed 1765, low tm 18247, high tm 16690, high any compl 3, high end compl 13, high repeat similarity 16, long poly-x seq 94, ok 9978
PRIMER_PAIR_EXPLAIN=considered 1765, unacceptable product size 1716, high end compl 5, ok 44
PRIMER_PAIR_PENALTY=0.1062
PRIMER_LEFT_PENALTY=0.048359
PRIMER_RIGHT_PENALTY=0.057845
PRIMER_LEFT_SEQUENCE=TTTATTATGAACCGCAATATGGG
PRIMER_RIGHT_SEQUENCE=AACCAGTGCTATCATCACAACCT
PRIMER_LEFT=3660,23
PRIMER_RIGHT=3960,23
PRIMER_LEFT_TM=59.952
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=301
PRIMER_PAIR_PENALTY_1=0.1483
PRIMER_LEFT_1_PENALTY=0.132718
PRIMER_RIGHT_1_PENALTY=0.015613
PRIMER_LEFT_1_SEQUENCE=CTCCTGTGACATTATGTGGATCA
PRIMER_RIGHT_1_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_1=937,23
PRIMER_RIGHT_1=1166,23
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=59.984
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER11B a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1940
PRIMER_LEFT_2_PENALTY=0.178426
PRIMER_RIGHT_2_PENALTY=0.015613
PRIMER_LEFT_2_SEQUENCE=ACTCAGGATTTGTACTGCCTGAA
PRIMER_RIGHT_2_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_2=992,23
PRIMER_RIGHT_2=1166,23
PRIMER_LEFT_2_TM=60.178
PRIMER_RIGHT_2_TM=59.984
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.1940
PRIMER_LEFT_3_PENALTY=0.178426
PRIMER_RIGHT_3_PENALTY=0.015613
PRIMER_LEFT_3_SEQUENCE=GTTGAAGCTTATGTGGGACTGAG
PRIMER_RIGHT_3_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_3=740,23
PRIMER_RIGHT_3=1166,23
PRIMER_LEFT_3_TM=60.178
PRIMER_RIGHT_3_TM=59.984
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=427
PRIMER_PAIR_PENALTY_4=0.1979
PRIMER_LEFT_4_PENALTY=0.116415
PRIMER_RIGHT_4_PENALTY=0.081493
PRIMER_LEFT_4_SEQUENCE=TGTGGAGGAAGGAAAGTCTATCA
PRIMER_RIGHT_4_SEQUENCE=GTGTGTGGCTTGTTTCATTCATA
PRIMER_LEFT_4=1102,23
PRIMER_RIGHT_4=1220,23
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=119
PRIMER_PAIR_PENALTY_5=0.2119
PRIMER_LEFT_5_PENALTY=0.116415
PRIMER_RIGHT_5_PENALTY=0.095470
PRIMER_LEFT_5_SEQUENCE=TGTGGAGGAAGGAAAGTCTATCA
PRIMER_RIGHT_5_SEQUENCE=GGTCATTCCAATAATGACAGCAT
PRIMER_LEFT_5=1102,23
PRIMER_RIGHT_5=2047,23
PRIMER_LEFT_5_TM=60.116
PRIMER_RIGHT_5_TM=60.095
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=7.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=3.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.9000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=946
PRIMER_PAIR_PENALTY_6=0.2142
PRIMER_LEFT_6_PENALTY=0.132718
PRIMER_RIGHT_6_PENALTY=0.081493
PRIMER_LEFT_6_SEQUENCE=CTCCTGTGACATTATGTGGATCA
PRIMER_RIGHT_6_SEQUENCE=GTGTGTGGCTTGTTTCATTCATA
PRIMER_LEFT_6=937,23
PRIMER_RIGHT_6=1220,23
PRIMER_LEFT_6_TM=59.867
PRIMER_RIGHT_6_TM=59.919
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER11B a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=19.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_6_END_STABILITY=6.6000
PRIMER_RIGHT_6_END_STABILITY=5.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=284
PRIMER_PAIR_PENALTY_7=0.2341
PRIMER_LEFT_7_PENALTY=0.193896
PRIMER_RIGHT_7_PENALTY=0.040223
PRIMER_LEFT_7_SEQUENCE=TCTGCATAGACAAAGGCCTTAAC
PRIMER_RIGHT_7_SEQUENCE=TTCCTAAGTAGGGACTGACACCA
PRIMER_LEFT_7=3342,23
PRIMER_RIGHT_7=3733,23
PRIMER_LEFT_7_TM=59.806
PRIMER_RIGHT_7_TM=60.040
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER4B a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.0000
PRIMER_RIGHT_7_END_STABILITY=8.2000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=392
PRIMER_PAIR_PENALTY_8=0.2399
PRIMER_LEFT_8_PENALTY=0.224301
PRIMER_RIGHT_8_PENALTY=0.015613
PRIMER_LEFT_8_SEQUENCE=GAAGCTTATGTGGGACTGAGAAA
PRIMER_RIGHT_8_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_8=743,23
PRIMER_RIGHT_8=1166,23
PRIMER_LEFT_8_TM=59.776
PRIMER_RIGHT_8_TM=59.984
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.0000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=424
PRIMER_PAIR_PENALTY_9=0.2517
PRIMER_LEFT_9_PENALTY=0.193896
PRIMER_RIGHT_9_PENALTY=0.057845
PRIMER_LEFT_9_SEQUENCE=TCTGCATAGACAAAGGCCTTAAC
PRIMER_RIGHT_9_SEQUENCE=AACCAGTGCTATCATCACAACCT
PRIMER_LEFT_9=3342,23
PRIMER_RIGHT_9=3960,23
PRIMER_LEFT_9_TM=59.806
PRIMER_RIGHT_9_TM=59.942
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.0000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=619
PRIMER_PAIR_PENALTY_10=0.2545
PRIMER_LEFT_10_PENALTY=0.159038
PRIMER_RIGHT_10_PENALTY=0.095470
PRIMER_LEFT_10_SEQUENCE=AATGCTGTTTCTGCTTAAGTTGG
PRIMER_RIGHT_10_SEQUENCE=GGTCATTCCAATAATGACAGCAT
PRIMER_LEFT_10=1828,23
PRIMER_RIGHT_10=2047,23
PRIMER_LEFT_10_TM=59.841
PRIMER_RIGHT_10_TM=60.095
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.2000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=220
PRIMER_PAIR_PENALTY_11=0.2599
PRIMER_LEFT_11_PENALTY=0.178426
PRIMER_RIGHT_11_PENALTY=0.081493
PRIMER_LEFT_11_SEQUENCE=GTTGAAGCTTATGTGGGACTGAG
PRIMER_RIGHT_11_SEQUENCE=GTGTGTGGCTTGTTTCATTCATA
PRIMER_LEFT_11=740,23
PRIMER_RIGHT_11=1220,23
PRIMER_LEFT_11_TM=60.178
PRIMER_RIGHT_11_TM=59.919
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=5.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=481
PRIMER_PAIR_PENALTY_12=0.2635
PRIMER_LEFT_12_PENALTY=0.193896
PRIMER_RIGHT_12_PENALTY=0.069594
PRIMER_LEFT_12_SEQUENCE=TCTGCATAGACAAAGGCCTTAAC
PRIMER_RIGHT_12_SEQUENCE=CTCCCATATTGCGGTTCATAATA
PRIMER_LEFT_12=3342,23
PRIMER_RIGHT_12=3684,23
PRIMER_LEFT_12_TM=59.806
PRIMER_RIGHT_12_TM=60.070
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=8.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.0000
PRIMER_RIGHT_12_END_STABILITY=5.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=343
PRIMER_PAIR_PENALTY_13=0.2660
PRIMER_LEFT_13_PENALTY=0.250358
PRIMER_RIGHT_13_PENALTY=0.015613
PRIMER_LEFT_13_SEQUENCE=CAATTTTACCCGAAACAAACTGA
PRIMER_RIGHT_13_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_13=841,23
PRIMER_RIGHT_13=1166,23
PRIMER_LEFT_13_TM=60.250
PRIMER_RIGHT_13_TM=59.984
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=326
PRIMER_PAIR_PENALTY_14=0.2660
PRIMER_LEFT_14_PENALTY=0.250358
PRIMER_RIGHT_14_PENALTY=0.015613
PRIMER_LEFT_14_SEQUENCE=TCAATTTTACCCGAAACAAACTG
PRIMER_RIGHT_14_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_14=840,23
PRIMER_RIGHT_14=1166,23
PRIMER_LEFT_14_TM=60.250
PRIMER_RIGHT_14_TM=59.984
PRIMER_LEFT_14_GC_PERCENT=34.783
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=327
PRIMER_PAIR_PENALTY_15=0.2755
PRIMER_LEFT_15_PENALTY=0.116415
PRIMER_RIGHT_15_PENALTY=0.159038
PRIMER_LEFT_15_SEQUENCE=TGTGGAGGAAGGAAAGTCTATCA
PRIMER_RIGHT_15_SEQUENCE=CCAACTTAAGCAGAAACAGCATT
PRIMER_LEFT_15=1102,23
PRIMER_RIGHT_15=1850,23
PRIMER_LEFT_15_TM=60.116
PRIMER_RIGHT_15_TM=59.841
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=5.9000
PRIMER_RIGHT_15_END_STABILITY=8.4000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=749
PRIMER_PAIR_PENALTY_16=0.2762
PRIMER_LEFT_16_PENALTY=0.260543
PRIMER_RIGHT_16_PENALTY=0.015613
PRIMER_LEFT_16_SEQUENCE=ACTCTCCAAGAGGCTAAATCCAG
PRIMER_RIGHT_16_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_16=962,23
PRIMER_RIGHT_16=1166,23
PRIMER_LEFT_16_TM=60.261
PRIMER_RIGHT_16_TM=59.984
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=205
PRIMER_PAIR_PENALTY_17=0.2765
PRIMER_LEFT_17_PENALTY=0.160134
PRIMER_RIGHT_17_PENALTY=0.116415
PRIMER_LEFT_17_SEQUENCE=CCTTGACTTGTCTGAACTGATCC
PRIMER_RIGHT_17_SEQUENCE=TGATAGACTTTCCTTCCTCCACA
PRIMER_LEFT_17=892,23
PRIMER_RIGHT_17=1124,23
PRIMER_LEFT_17_TM=60.160
PRIMER_RIGHT_17_TM=60.116
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=5.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.8000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=233
PRIMER_PAIR_PENALTY_18=0.2788
PRIMER_LEFT_18_PENALTY=0.263142
PRIMER_RIGHT_18_PENALTY=0.015613
PRIMER_LEFT_18_SEQUENCE=ATCCATTTACATGCTCCTGTGAC
PRIMER_RIGHT_18_SEQUENCE=ACATATTAGGAACCGGATCACCT
PRIMER_LEFT_18=924,23
PRIMER_RIGHT_18=1166,23
PRIMER_LEFT_18_TM=60.263
PRIMER_RIGHT_18_TM=59.984
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.1000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=243
PRIMER_PAIR_PENALTY_19=0.2887
PRIMER_LEFT_19_PENALTY=0.116415
PRIMER_RIGHT_19_PENALTY=0.172268
PRIMER_LEFT_19_SEQUENCE=TGTGGAGGAAGGAAAGTCTATCA
PRIMER_RIGHT_19_SEQUENCE=GATTTTCAATGACAGGGATCTTG
PRIMER_LEFT_19=1102,23
PRIMER_RIGHT_19=2069,23
PRIMER_LEFT_19_TM=60.116
PRIMER_RIGHT_19_TM=59.828
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=968
=