PRIMER_SEQUENCE_ID=NM_006206
SEQUENCE=TTCTCCCCGCCCCCCAGTTGTTGTCGAAGTCTGGGGGTTGGGACTGGACCCCCTGATTGCGTAAGAGCAAAAAGCGAAGGCGCAATCTGGACACTGGGAGATTCGGAGCGCAGGGAGTTTGAGAGAAACTTTTATTTTGAAGAGACCAAGGTTGAGGGGGGGCTTATTTCCTGACAGCTATTTACTTAGAGCAAATGATTAGTTTTAGAAGGATGGACTATAACATTGAATCAATTACAAAACGCGGTTTTTGAGCCCATTACTGTTGGAGCTACAGGGAGAGAAACAGGAGGAGACTGCAAGAGATCATTTGGGAAGGCCGTGGGCACGCTCTTTACTCCATGTGTGGGACATTCATTGCGGAATAACATCGGAGGAGAAGTTTCCCAGAGCTATGGGGACTTCCCATCCGGCGTTCCTGGTCTTAGGCTGTCTTCTCACAGGGCTGAGCCTAATCCTCTGCCAGCTTTCATTACCCTCTATCCTTCCAAATGAAAATGAAAAGGTTGTGCAGCTGAATTCATCCTTTTCTCTGAGATGCTTTGGGGAGAGTGAAGTGAGCTGGCAGTACCCCATGTCTGAAGAAGAGAGCTCCGATGTGGAAATCAGAAATGAAGAAAACAACAGCGGCCTTTTTGTGACGGTCTTGGAAGTGAGCAGTGCCTCGGCGGCCCACACAGGGTTGTACACTTGCTATTACAACCACACTCAGACAGAAGAGAATGAGCTTGAAGGCAGGCACATTTACATCTATGTGCCAGACCCAGATGTAGCCTTTGTACCTCTAGGAATGACGGATTATTTAGTCATCGTGGAGGATGATGATTCTGCCATTATACCTTGTCGCACAACTGATCCCGAGACTCCTGTAACCTTACACAACAGTGAGGGGGTGGTACCTGCCTCCTACGACAGCAGACAGGGCTTTAATGGGACCTTCACTGTAGGGCCCTATATCTGTGAGGCCACCGTCAAAGGAAAGAAGTTCCAGACCATCCCATTTAATGTTTATGCTTTAAAAGCAACATCAGAGCTGGATCTAGAAATGGAAGCTCTTAAAACCGTGTATAAGTCAGGGGAAACGATTGTGGTCACCTGTGCTGTTTTTAACAATGAGGTGGTTGACCTTCAATGGACTTACCCTGGAGAAGTGAAAGGCAAAGGCATCACAATGCTGGAAGAAATCAAAGTCCCATCCATCAAATTGGTGTACACTTTGACGGTCCCCGAGGCCACGGTGAAAGACAGTGGAGATTACGAATGTGCTGCCCGCCAGGCTACCAGGGAGGTCAAAGAAATGAAGAAAGTCACTATTTCTGTCCATGAGAAAGGTTTCATTGAAATCAAACCCACCTTCAGCCAGTTGGAAGCTGTCAACCTGCATGAAGTCAAACATTTTGTTGTAGAGGTGCGGGCCTACCCACCTCCCAGGATATCCTGGCTGAAAAACAATCTGACTCTGATTGAAAATCTCACTGAGATCACCACTGATGTGGAAAAGATTCAGGAAATAAGGTATCGAAGCAAATTAAAGCTGATCCGTGCTAAGGAAGAAGACAGTGGCCATTATACTATTGTAGCTCAAAATGAAGATGCTGTGAAGAGCTATACTTTTGAACTGTTAACTCAAGTTCCTTCATCCATTCTGGACTTGGTCGATGATCACCATGGCTCAACTGGGGGACAGACGGTGAGGTGCACAGCTGAAGGCACGCCGCTTCCTGATATTGAGTGGATGATATGCAAAGATATTAAGAAATGTAATAATGAAACTTCCTGGACTATTTTGGCCAACAATGTCTCAAACATCATCACGGAGATCCACTCCCGAGACAGGAGTACCGTGGAGGGCCGTGTGACTTTCGCCAAAGTGGAGGAGACCATCGCCGTGCGATGCCTGGCTAAGAATCTCCTTGGAGCTGAGAACCGAGAGCTGAAGCTGGTGGCTCCCACCCTGCGTTCTGAACTCACGGTGGCTGCTGCAGTCCTGGTGCTGTTGGTGATTGTGATCATCTCACTTATTGTCCTGGTTGTCATTTGGAAACAGAAACCGAGGTATGAAATTCGCTGGAGGGTCATTGAATCAATCAGCCCGGATGGACATGAATATATTTATGTGGACCCGATGCAGCTGCCTTATGACTCAAGATGGGAGTTTCCAAGAGATGGACTAGTGCTTGGTCGGGTCTTGGGGTCTGGAGCGTTTGGGAAGGTGGTTGAAGGAACAGCCTATGGATTAAGCCGGTCCCAACCTGTCATGAAAGTTGCAGTGAAGATGCTAAAACCCACGGCCAGATCCAGTGAAAAACAAGCTCTCATGTCTGAACTGAAGATAATGACTCACCTGGGGCCACATTTGAACATTGTAAACTTGCTGGGAGCCTGCACCAAGTCAGGCCCCATTTACATCATCACAGAGTATTGCTTCTATGGAGATTTGGTCAACTATTTGCATAAGAATAGGGATAGCTTCCTGAGCCACCACCCAGAGAAGCCAAAGAAAGAGCTGGATATCTTTGGATTGAACCCTGCTGATGAAAGCACACGGAGCTATGTTATTTTATCTTTTGAAAACAATGGTGACTACATGGACATGAAGCAGGCTGATACTACACAGTATGTCCCCATGCTAGAAAGGAAAGAGGTTTCTAAATATTCCGACATCCAGAGATCACTCTATGATCGTCCAGCCTCATATAAGAAGAAATCTATGTTAGACTCAGAAGTCAAAAACCTCCTTTCAGATGATAACTCAGAAGGCCTTACTTTATTGGATTTGTTGAGCTTCACCTATCAAGTTGCCCGAGGAATGGAGTTTTTGGCTTCAAAAAATTGTGTCCACCGTGATCTGGCTGCTCGCAACGTCCTCCTGGCACAAGGAAAAATTGTGAAGATCTGTGACTTTGGCCTGGCCAGAGACATCATGCATGATTCGAACTATGTGTCGAAAGGCAGTACCTTTCTGCCCGTGAAGTGGATGGCTCCTGAGAGCATCTTTGACAACCTCTACACCACACTGAGTGATGTCTGGTCTTATGGCATTCTGCTCTGGGAGATCTTTTCCCTTGGTGGCACCCCTTACCCCGGCATGATGGTGGATTCTACTTTCTACAATAAGATCAAGAGTGGGTACCGGATGGCCAAGCCTGACCACGCTACCAGTGAAGTCTACGAGATCATGGTGAAATGCTGGAACAGTGAGCCGGAGAAGAGACCCTCCTTTTACCACCTGAGTGAGATTGTGGAGAATCTGCTGCCTGGACAATATAAAAAGAGTTATGAAAAAATTCACCTGGACTTCCTGAAGAGTGACCATCCTGCTGTGGCACGCATGCGTGTGGACTCAGACAATGCATACATTGGTGTCACCTACAAAAACGAGGAAGACAAGCTGAAGGACTGGGAGGGTGGTCTGGATGAGCAGAGACTGAGCGCTGACAGTGGCTACATCATTCCTCTGCCTGACATTGACCCTGTCCCTGAGGAGGAGGACCTGGGCAAGAGGAACAGACACAGCTCGCAGACCTCTGAAGAGAGTGCCATTGAGACGGGTTCCAGCAGTTCCACCTTCATCAAGAGAGAGGACGAGACCATTGAAGACATCGACATGATGGACGACATCGGCATAGACTCTTCAGACCTGGTGGAAGACAGCTTCCTGTAACTGGCGGATTCGAGGGGTTCCTTCCACTTCTGGGGCCACCTCTGGATCCCGTTCAGAAAACCACTTTATTGCAATGCGGAGGTTGAGAGGAGGACTTGGTTGATGTTTAAAGAGAAGTTCCCAGCCAAGGGCCTCGGGGAGCGTTCTAAATATGAATGAATGGGATATTTTGAAATGAACTTTGTCAGTGTTGCCTCTCGCAATGCCTCAGTAGCATCTCAGTGGTGTGTGAAGTTTGGAGATAGATGGATAAGGGAATAATAGGCCACAGAAGGTGAACTTTGTGCTTCAAGGACATTGGTGAGAGTCCAACAGACACAATTTATACTGCGACAGAACTTCAGCATTGTAATTATGTAAATAACTCTAACCAAGGCTGTGTTTAGATTGTATTAACTATCTTCTTTGGACTTCTGAAGAGACCACTCAATCCATCCATGTACTTCCCTCTTGAAACCTGATGTCAGCTGCTGTTGAACTTTTTAAAGAAGTGCATGAAAAACCATTTTTGAACCTTAAAAGGTACTGGTACTATAGCATTTTGCTATCTTTTTTAGTGTTAAGAGATAAAGAATAATAATTAACCAACCTTGTTTAATAGATTTGGGTCATTTAGAAGCCTGACAACTCATTTTCATATTGTAATCTATGTTTATAATACTACTACTGTTATCAGTAATGCTAAATGTGTAATAATGTAACATGATTTCCCTCCAGAGAAAGCACAATTTAAAACAATCCTTACTAAGTAGGTGATGAGTTTGACAGTTTTTGACATTTATATTAAATAACATGTTTCTCTATAAAGTATGGTAATAGCTTTAGTGAATTAAATTTAGTTGAGCATAGAGAACAAAGTAAAAGTAGTGTTGTCCAGGAAGTCAGAATTTTTAACTGTACTGAATAGGTTCCCCAATCCATCGTATTAAAAAACAATTAACTGCCCTCTGAAATAATGGGATTAGAAACAAACAAAACTCTTAAGTCCTAAAAGTTCTCAATGTAGAGGCATAAACCTGTGCTGAACATAACTTCTCATGTATATTACCCAATGGAAAATATAATGATCAGCAAAAAGACTGGATTTGCAGAAGTTTTTTTTTTTTTTCTTCATGCCTGATGAAAGCTTTGGCAACCCCAATATATGTATTTTTTGAATCTATGAACCTGAAAAGGGTCAGAAGGATGCCCAGACATCAGCCTCCTTCTTTCACCCCTTACCCCAAAGAGAAAGAGTTTGAAACTCGAGACCATAAAGATATTCTTTAGTGGAGGCTGGATGTGCATTAGCCTGGATCCTCAGTTCTCAAATGTGTGTGGCAGCCAGGATGACTAGATCCTGGGTTTCCATCCTTGAGATTCTGAAGTATGAAGTCTGAGGGAAACCAGAGTCTGTATTTTTCTAAACTCCCTGGCTGTTCTGATCGGCCAGTTTTCGGAAACACTGACTTAGGTTTCAGGAAGTTGCCATGGGAAACAAATAATTTGAACTTTGGAACAGGGTTGGAATTCAACCACGCAGGAAGCCTACTATTTAAATCCTTGGCTTCAGGTTAGTGACATTTAATGCCATCTAGCTAGCAATTGCGACCTTAATTTAACTTTCCAGTCTTAGCTGAGGCTGAGAAAGCTAAAGTTTGGTTTTGACAGGTTTTCCAAAAGTAAAGATGCTACTTCCCACTGTATGGGGGAGATTGAACTTTCCCCGTCTCCCGTCTTCTGCCTCCCACTCCATACCCCGCCAAGGAAAGGCATGTACAAAAATTATGCAATTCAGTGTTCCAAGTCTCTGTGTAACCAGCTCAGTGTTTTGGTGGAAAAAACATTTTAAGTTTTACTGATAATTTGAGGTTAGATGGGAGGATGAATTGTCACATCTATCCACACTGTCAAACAGGTTGGTGTGGGTTCATTGGCATTCTTTGCAATACTGCTTAATTGCTGATACCATATGAATGAAACATGGGCTGTGATTACTGCAATCACTGTGCTATCGGCAGATGATGCTTTGGAAGATGCAGAAGCAATAATAAAGTACTTGACTACCTACTGGTGTAATCTCAATGCAAGCCCCAACTTTCTTATCCAACTTTTTCATAGTAAGTGCGAAGACTGAGCCAGATTGGCCAATTAAAAACGAAAACCTGACTAGGTTCTGTAGAGCCAATTAGACTTGAAATACGTTTGTGTTTCTAGAATCACAGCTCAAGCATTCTGTTTATCGCTCACTCTCCCTTGTACAGCCTTATTTTGTTGGTGCTTTGCATTTTGATATTGCTGTGAGCCTTGCATGACATCATGAGGCCGGATGAAACTTCTCAGTCCAGCAGTTTCCAGTCCTAACAAATGCTCCCACCTGAATTTGTATATGACTGCATTTGTGGGTGTGTGTGTGTTTTCAGCAAATTCCAGATTTGTTTCCTTTTGGCCTCCTGCAAAGTCTCCAGAAGAAAATTTGCCAATCTTTCCTACTTTCTATTTTTATGATGACAATCAAAGCCGGCCTGAGAAACACTATTTGTGACTTTTTAAACGATTAGTGATGTCCTTAAAATGTGGTCTGCCAATCTGTACAAAATGGTCCTATTTTTGTGAAGAGGGACATAAGATAAAATGATGTTATACATCAATATGTATATATGTATTTCTATATAGACTTGGAGAATACTGCCAAAACATTTATGACAAGCTGTATCACTGCCTTCGTTTATATTTTTTTAACTGTGATAATCCCCACAGGCACATTAACTGTTGCACTTTTGAATGTCCAAAATTTATATTTTAGAAATAATAAAAAGAAAGATACTTACATGTTCCCAAAACAATGGTGTGGTGAATGTGTGAGAAAAACTAACTTGATAGGGTCTACCAATACAAAATGTATTACGAATGCCCCTGTTCATGTTTTTGTTTTAAAACGTGTAAATGAAGATCTTTATATTTCAATAAATGATATATAATTTAAAGTT
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 77011, GC content failed 2489, low tm 38469, high tm 18631, high any compl 10, high end compl 45, high repeat similarity 46, long poly-x seq 211, ok 17110
PRIMER_RIGHT_EXPLAIN=considered 77145, GC content failed 2895, low tm 38677, high tm 18231, high any compl 1, high end compl 11, high repeat similarity 12, long poly-x seq 184, ok 17134
PRIMER_PAIR_EXPLAIN=considered 1717, unacceptable product size 1643, high end compl 8, ok 66
PRIMER_PAIR_PENALTY=0.0146
PRIMER_LEFT_PENALTY=0.009608
PRIMER_RIGHT_PENALTY=0.004943
PRIMER_LEFT_SEQUENCE=ACTGATGTGGAAAAGATTCAGGA
PRIMER_RIGHT_SEQUENCE=CCCAGGTGAGTCATTATCTTCAG
PRIMER_LEFT=1486,23
PRIMER_RIGHT=2348,23
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.005
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MER5b a consensus
PRIMER_PAIR_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=863
PRIMER_PAIR_PENALTY_1=0.0164
PRIMER_LEFT_1_PENALTY=0.009608
PRIMER_RIGHT_1_PENALTY=0.006805
PRIMER_LEFT_1_SEQUENCE=ACTGATGTGGAAAAGATTCAGGA
PRIMER_RIGHT_1_SEQUENCE=GCTTAATCCATAGGCTGTTCCTT
PRIMER_LEFT_1=1486,23
PRIMER_RIGHT_1=2241,23
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.007
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, KER Human K element interspersed repeat DNA
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=756
PRIMER_PAIR_PENALTY_2=0.0281
PRIMER_LEFT_2_PENALTY=0.023182
PRIMER_RIGHT_2_PENALTY=0.004943
PRIMER_LEFT_2_SEQUENCE=TTGAAGGAACAGCCTATGGATTA
PRIMER_RIGHT_2_SEQUENCE=CCCAGGTGAGTCATTATCTTCAG
PRIMER_LEFT_2=2216,23
PRIMER_RIGHT_2=2348,23
PRIMER_LEFT_2_TM=59.977
PRIMER_RIGHT_2_TM=60.005
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MER5b a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.0328
PRIMER_LEFT_3_PENALTY=0.009608
PRIMER_RIGHT_3_PENALTY=0.023182
PRIMER_LEFT_3_SEQUENCE=ACTGATGTGGAAAAGATTCAGGA
PRIMER_RIGHT_3_SEQUENCE=TAATCCATAGGCTGTTCCTTCAA
PRIMER_LEFT_3=1486,23
PRIMER_RIGHT_3=2238,23
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=59.977
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=753
PRIMER_PAIR_PENALTY_4=0.0358
PRIMER_LEFT_4_PENALTY=0.016142
PRIMER_RIGHT_4_PENALTY=0.019662
PRIMER_LEFT_4_SEQUENCE=CTCAGTAGCATCTCAGTGGTGTG
PRIMER_RIGHT_4_SEQUENCE=TACATATATTGGGGTTGCCAAAG
PRIMER_LEFT_4=3870,23
PRIMER_RIGHT_4=4812,23
PRIMER_LEFT_4_TM=59.984
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=943
PRIMER_PAIR_PENALTY_5=0.0364
PRIMER_LEFT_5_PENALTY=0.023182
PRIMER_RIGHT_5_PENALTY=0.013200
PRIMER_LEFT_5_SEQUENCE=TTGAAGGAACAGCCTATGGATTA
PRIMER_RIGHT_5_SEQUENCE=CTCGTAGACTTCACTGGTAGCGT
PRIMER_LEFT_5=2216,23
PRIMER_RIGHT_5=3174,23
PRIMER_LEFT_5_TM=59.977
PRIMER_RIGHT_5_TM=60.013
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.9000
PRIMER_RIGHT_5_END_STABILITY=9.6000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=959
PRIMER_PAIR_PENALTY_6=0.0421
PRIMER_LEFT_6_PENALTY=0.016142
PRIMER_RIGHT_6_PENALTY=0.026007
PRIMER_LEFT_6_SEQUENCE=CTCAGTAGCATCTCAGTGGTGTG
PRIMER_RIGHT_6_SEQUENCE=AAATTCTGACTTCCTGGACAACA
PRIMER_LEFT_6=3870,23
PRIMER_RIGHT_6=4553,23
PRIMER_LEFT_6_TM=59.984
PRIMER_RIGHT_6_TM=60.026
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=7.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=684
PRIMER_PAIR_PENALTY_7=0.0457
PRIMER_LEFT_7_PENALTY=0.026007
PRIMER_RIGHT_7_PENALTY=0.019662
PRIMER_LEFT_7_SEQUENCE=TGTTGTCCAGGAAGTCAGAATTT
PRIMER_RIGHT_7_SEQUENCE=TACATATATTGGGGTTGCCAAAG
PRIMER_LEFT_7=4531,23
PRIMER_RIGHT_7=4812,23
PRIMER_LEFT_7_TM=60.026
PRIMER_RIGHT_7_TM=59.980
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.2000
PRIMER_RIGHT_7_END_STABILITY=7.3000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=282
PRIMER_PAIR_PENALTY_8=0.0513
PRIMER_LEFT_8_PENALTY=0.023182
PRIMER_RIGHT_8_PENALTY=0.028128
PRIMER_LEFT_8_SEQUENCE=TTGAAGGAACAGCCTATGGATTA
PRIMER_RIGHT_8_SEQUENCE=CTTTCTAGCATGGGGACATACTG
PRIMER_LEFT_8=2216,23
PRIMER_RIGHT_8=2636,23
PRIMER_LEFT_8_TM=59.977
PRIMER_RIGHT_8_TM=60.028
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=5.9000
PRIMER_RIGHT_8_END_STABILITY=5.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=421
PRIMER_PAIR_PENALTY_9=0.0516
PRIMER_LEFT_9_PENALTY=0.048411
PRIMER_RIGHT_9_PENALTY=0.003218
PRIMER_LEFT_9_SEQUENCE=TTGGCTTCAGGTTAGTGACATTT
PRIMER_RIGHT_9_SEQUENCE=AACAAATCTGGAATTTGCTGAAA
PRIMER_LEFT_9=5206,23
PRIMER_RIGHT_9=6082,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=59.997
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=30.435
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=7.3000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=877
PRIMER_PAIR_PENALTY_10=0.0528
PRIMER_LEFT_10_PENALTY=0.003045
PRIMER_RIGHT_10_PENALTY=0.049761
PRIMER_LEFT_10_SEQUENCE=AATCTGCTGCCTGGACAATATAA
PRIMER_RIGHT_10_SEQUENCE=GCAATAAAGTGGTTTTCTGAACG
PRIMER_LEFT_10=3247,23
PRIMER_RIGHT_10=3735,23
PRIMER_LEFT_10_TM=60.003
PRIMER_RIGHT_10_TM=60.050
PRIMER_LEFT_10_GC_PERCENT=39.130
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.2000
PRIMER_RIGHT_10_END_STABILITY=8.4000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=489
PRIMER_PAIR_PENALTY_11=0.0563
PRIMER_LEFT_11_PENALTY=0.006805
PRIMER_RIGHT_11_PENALTY=0.049500
PRIMER_LEFT_11_SEQUENCE=GGGCTTATTTCCTGACAGCTATT
PRIMER_RIGHT_11_SEQUENCE=CTCTCCCTGTAGCTCCAACAGTA
PRIMER_LEFT_11=159,23
PRIMER_RIGHT_11=282,23
PRIMER_LEFT_11_TM=60.007
PRIMER_RIGHT_11_TM=59.950
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=52.174
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.9000
PRIMER_RIGHT_11_END_STABILITY=5.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=124
PRIMER_PAIR_PENALTY_12=0.0566
PRIMER_LEFT_12_PENALTY=0.046971
PRIMER_RIGHT_12_PENALTY=0.009608
PRIMER_LEFT_12_SEQUENCE=CGGTGAAAGACAGTGGAGATTAC
PRIMER_RIGHT_12_SEQUENCE=TCCTGAATCTTTTCCACATCAGT
PRIMER_LEFT_12=1235,23
PRIMER_RIGHT_12=1508,23
PRIMER_LEFT_12_TM=60.047
PRIMER_RIGHT_12_TM=59.990
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.6000
PRIMER_RIGHT_12_END_STABILITY=6.4000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=274
PRIMER_PAIR_PENALTY_13=0.0566
PRIMER_LEFT_13_PENALTY=0.046971
PRIMER_RIGHT_13_PENALTY=0.009608
PRIMER_LEFT_13_SEQUENCE=GGTGAAAGACAGTGGAGATTACG
PRIMER_RIGHT_13_SEQUENCE=TCCTGAATCTTTTCCACATCAGT
PRIMER_LEFT_13=1236,23
PRIMER_RIGHT_13=1508,23
PRIMER_LEFT_13_TM=60.047
PRIMER_RIGHT_13_TM=59.990
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.7000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=273
PRIMER_PAIR_PENALTY_14=0.0566
PRIMER_LEFT_14_PENALTY=0.046971
PRIMER_RIGHT_14_PENALTY=0.009608
PRIMER_LEFT_14_SEQUENCE=ACGGTGAAAGACAGTGGAGATTA
PRIMER_RIGHT_14_SEQUENCE=TCCTGAATCTTTTCCACATCAGT
PRIMER_LEFT_14=1234,23
PRIMER_RIGHT_14=1508,23
PRIMER_LEFT_14_TM=60.047
PRIMER_RIGHT_14_TM=59.990
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MER7B Nonautonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER1B Nonautonomous DNA transposon
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.9000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=275
PRIMER_PAIR_PENALTY_15=0.0570
PRIMER_LEFT_15_PENALTY=0.053769
PRIMER_RIGHT_15_PENALTY=0.003218
PRIMER_LEFT_15_SEQUENCE=ATGCAATTCAGTGTTCCAAGTCT
PRIMER_RIGHT_15_SEQUENCE=AAATCTGGAATTTGCTGAAAACA
PRIMER_LEFT_15=5430,23
PRIMER_RIGHT_15=6079,23
PRIMER_LEFT_15_TM=60.054
PRIMER_RIGHT_15_TM=59.997
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=30.435
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=650
PRIMER_PAIR_PENALTY_16=0.0570
PRIMER_LEFT_16_PENALTY=0.053769
PRIMER_RIGHT_16_PENALTY=0.003218
PRIMER_LEFT_16_SEQUENCE=ATGCAATTCAGTGTTCCAAGTCT
PRIMER_RIGHT_16_SEQUENCE=AACAAATCTGGAATTTGCTGAAA
PRIMER_LEFT_16=5430,23
PRIMER_RIGHT_16=6082,23
PRIMER_LEFT_16_TM=60.054
PRIMER_RIGHT_16_TM=59.997
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=30.435
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=6.1000
PRIMER_RIGHT_16_END_STABILITY=7.3000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=653
PRIMER_PAIR_PENALTY_17=0.0570
PRIMER_LEFT_17_PENALTY=0.053769
PRIMER_RIGHT_17_PENALTY=0.003218
PRIMER_LEFT_17_SEQUENCE=ATGCAATTCAGTGTTCCAAGTCT
PRIMER_RIGHT_17_SEQUENCE=AAACAAATCTGGAATTTGCTGAA
PRIMER_LEFT_17=5430,23
PRIMER_RIGHT_17=6083,23
PRIMER_LEFT_17_TM=60.054
PRIMER_RIGHT_17_TM=59.997
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=30.435
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MARINER1 Human mariner
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=654
PRIMER_PAIR_PENALTY_18=0.0580
PRIMER_LEFT_18_PENALTY=0.009608
PRIMER_RIGHT_18_PENALTY=0.048411
PRIMER_LEFT_18_SEQUENCE=ACTGATGTGGAAAAGATTCAGGA
PRIMER_RIGHT_18_SEQUENCE=CTCCCAGCAAGTTTACAATGTTC
PRIMER_LEFT_18=1486,23
PRIMER_RIGHT_18=2380,23
PRIMER_LEFT_18_TM=59.990
PRIMER_RIGHT_18_TM=60.048
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, SVA Composite retroposon
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=895
PRIMER_PAIR_PENALTY_19=0.0581
PRIMER_LEFT_19_PENALTY=0.023182
PRIMER_RIGHT_19_PENALTY=0.034931
PRIMER_LEFT_19_SEQUENCE=TTGAAGGAACAGCCTATGGATTA
PRIMER_RIGHT_19_SEQUENCE=ATATGAGGCTGGACGATCATAGA
PRIMER_LEFT_19=2216,23
PRIMER_RIGHT_19=2697,23
PRIMER_LEFT_19_TM=59.977
PRIMER_RIGHT_19_TM=59.965
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=5.9000
PRIMER_RIGHT_19_END_STABILITY=5.6000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=482
=