PRIMER_SEQUENCE_ID=NM_006293
SEQUENCE=CATTAGATCTTTACATGAAAGTAAAATTTATAAGATTTCTAGAAAGTCAAAAGATGATAACTATTTCTTAGGATACTAAAAGCACTCACATTATAGAAAAAAAATCAGTTAACTATACTCCACAAACATTAAAGGCTCCCTATAAAAAAACATTTTTAATAGGCAAGCCACAGAAAGGGCAAATATTAATAGTTTGCAATACATATGTATGAAAAGGAATTGAATCTAGAATATTTAACAAAGCTTTACAACTCAAAAAATACAAAGAAAATATTTTTCTTCCAATTGGCAAATTACTTAAACAGAACCTTCACAAAAGAAGATAAGAATGTTTAATAAACATTTGAAGCCATAATAATGACATCATTAGCCATGATGGAAATGCAAATTTAAGTACCACTTCACATCCACAAGAAAAAGATAAAAATAAAAGGACTGAGCTCACCAAACATTGGTGAGGATGTGGTAATACTGAAATTCTTGTACCGTGCTCCTGAGGGTATAACATATTACAGGATTTTTTTGAAAACTAGTGGTTCCTTATAAACTTAATGCCCTGGCAACCTCACACCTATTTACTTAAGAATGAAAGGGCCCCGCCCTCCTCCCTCCTCGCTCGCGGGCCGGGCCCGGCATGGTGCGGCGTCGCCGCCGATGGCGCTGAGGCGGAGCATGGGGCGGCCGGGGCTCCCGCCGCTGCCGCTGCCGCCGCCACCGCGGCTCGGGCTGCTGCTGGCGGAGTCCGCCGCCGCAGGTCTGAAGCTCATGGGAGCCCCGGTGAAGCTGACAGTGTCTCAGGGGCAGCCGGTGAAGCTCAACTGCAGTGTGGAGGGGATGGAGGAGCCTGACATCCAGTGGGTGAAGGATGGGGCTGTGGTCCAGAACTTGGACCAGTTGTACATCCCAGTCAGCGAGCAGCACTGGATCGGCTTCCTCAGCCTGAAGTCAGTGGAGCGCTCTGACGCCGGCCGGTACTGGTGCCAGGTGGAGGATGGGGGTGAAACCGAGATCTCCCAGCCAGTGTGGCTCACGGTAGAAGGTGTGCCATTTTTCACAGTGGAGCCAAAAGATCTGGCAGTGCCACCCAATGCCCCTTTCCAACTGTCTTGTGAGGCTGTGGGTCCCCCTGAACCTGTTACCATTGTCTGGTGGAGAGGAACTACGAAGATCGGGGGACCCGCTCCCTCTCCATCTGTTTTAAATGTAACAGGGGTGACCCAGAGCACCATGTTTTCCTGTGAAGCTCACAACCTAAAAGGCCTGGCCTCTTCTCGCACAGCCACTGTTCACCTTCAAGCACTGCCTGCAGCCCCCTTCAACATCACCGTGACAAAGCTTTCCAGCAGCAACGCTAGTGTGGCCTGGATGCCAGGTGCTGATGGCCGAGCTCTGCTACAGTCCTGTACAGTTCAGGTGACACAGGCCCCAGGAGGCTGGGAAGTCCTGGCTGTTGTGGTCCCTGTGCCCCCCTTTACCTGCCTGCTCCGGGACCTGGTGCCTGCCACCAACTACAGCCTCAGGGTGCGCTGTGCCAATGCCTTGGGGCCCTCTCCCTATGCTGACTGGGTGCCCTTTCAGACCAAGGGTCTAGCCCCAGCCAGCGCTCCCCAAAACCTCCATGCCATCCGCACAGATTCAGGCCTCATCTTGGAGTGGGAAGAAGTGATCCCCGAGGCCCCTTTGGAAGGCCCCCTGGGACCCTACAAACTGTCCTGGGTTCAAGACAATGGAACCCAGGATGAGCTGACAGTGGAGGGGACCAGGGCCAATTTGACAGGCTGGGATCCCCAAAAGGACCTGATCGTACGTGTGTGCGTCTCCAATGCAGTTGGCTGTGGACCCTGGAGTCAGCCACTGGTGGTCTCTTCTCATGACCGTGCAGGCCAGCAGGGCCCTCCTCACAGCCGCACATCCTGGGTACCTGTGGTCCTTGGTGTGCTAACGGCCCTGGTGACGGCTGCTGCCCTGGCCCTCATCCTGCTTCGAAAGAGACGGAAAGAGACGCGGTTTGGGCAAGCCTTTGACAGTGTCATGGCCCGGGGAGAGCCAGCCGTTCACTTCCGGGCAGCCCGGTCCTTCAATCGAGAAAGGCCCGAGCGCATCGAGGCCACATTGGACAGCTTGGGCATCAGCGATGAACTAAAGGAAAAACTGGAGGATGTGCTCATCCCAGAGCAGCAGTTCACCCTGGGCCGGATGTTGGGCAAAGGAGAGTTTGGTTCAGTGCGGGAGGCCCAGCTGAAGCAAGAGGATGGCTCCTTTGTGAAAGTGGCTGTGAAGATGCTGAAAGCTGACATCATTGCCTCAAGCGACATTGAAGAGTTCCTCAGGGAAGCAGCTTGCATGAAGGAGTTTGACCATCCACACGTGGCCAAACTTGTTGGGGTAAGCCTCCGGAGCAGGGCTAAAGGCCGTCTCCCCATCCCCATGGTCATCTTGCCCTTCATGAAGCATGGGGACCTGCATGCCTTCCTGCTCGCCTCCCGGATTGGGGAGAACCCCTTTAACCTACCCCTCCAGACCCTGATCCGGTTCATGGTGGACATTGCCTGCGGCATGGAGTACCTGAGCTCTCGGAACTTCATCCACCGAGACCTGGCTGCTCGGAATTGCATGCTGGCAGAGGACATGACAGTGTGTGTGGCTGACTTCGGACTCTCCCGGAAGATCTACAGTGGGGACTACTATCGTCAAGGCTGTGCCTCCAAACTGCCTGTCAAGTGGCTGGCCCTGGAGAGCCTGGCCGACAACCTGTATACTGTGCAGAGTGACGTGTGGGCGTTCGGGGTGACCATGTGGGAGATCATGACACGTGGGCAGACGCCATATGCTGGCATCGAAAACGCTGAGATTTACAACTACCTCATTGGCGGGAACCGCCTGAAACAGCCTCCGGAGTGTATGGAGGACGTGTATGATCTCATGTACCAGTGCTGGAGTGCTGACCCCAAGCAGCGCCCGAGCTTTACTTGTCTGCGAATGGAACTGGAGAACATCTTGGGCCAGCTGTCTGTGCTATCTGCCAGCCAGGACCCCTTATACATCAACATCGAGAGAGCTGAGGAGCCCACTGTGGGAGGCAGCCTGGAGCTACCTGGCAGGGATCAGCCCTACAGTGGGGCTGGGGATGGCAGTGGCATGGGGGCAGTGGGTGGCACTCCCAGTGACTGTCGGTACATACTCACCCCCGGAGGGCTGGCTGAGCAGCCAGGGCAGGCAGAGCACCAGCCAGAGAGTCCCCTCAATGAGACACAGAGGCTTTTGCTGCTGCAGCAAGGGCTACTGCCACACAGTAGCTGTTAGCCCACAGGCAGAGGGCATCGGGGCCATTTGGCCGGCTCTGGTGGCCACTGAGCTGGCTGACTAAGCCCCGTCTGACCCCAGCCCAGACAGCAAGGTGTGGAGGCTCCTGTGGTAGTCCTCCCAAGCTGTGCTGGGAAGCCCGGACTGACCAAATCACCCAATCCCAGTTCTTCCTGCAACCACTCTGTGGCCAGCCTGGCATCAGTTTAGGCCTTGGCTTGATGGAAGTGGGCCAGTCCTGGTTGTCTGAACCCAGGCAGCTGGCAGGAGTGGGGTGGTTATGTTTCCATGGTTACCATGGGTGTGGATGGCAGTGTGGGGAGGGCAGGTCCAGCTCTGTGGGCCCTACCCTCCTGCTGAGCTGCCCCTGCTGCTTAAGTGCATGCATTGAGCTGCCTCCAGCCTGGTGGCCCAGCTATTACCACACTTGGGGTTTAAATATCCAGGTGTGCCCCTCCAAGTCAGAAAGAGATGTCCTTGTAATATTCCCTTTTAGGTGAGGGTTGGTAAGGGGTTGGTATCTCAGGTCTGAATCTTCACCATCTTTCTGATTCCGCACCCTGCCTACGCCAGGAGAAGTTGAGGGGAGCATGCTTCCCTGCAGCTGACCGGGTCACACAAAGGCATGCTGGAGTACCCAGCCTATCAGGTGCCCCTCTTCCAAAGGCAGCGTGCCGAGCCAGCAAGAGGAAGGGGTGCTGTGAGGCTTGCCCAGGAGCAAGTGAGGCCGGAGAGGAGTTCAGGAACCCTTCTCCATACCCACAATCTGAGCACGCTACCAAATCTCAAAATATCCTAAGACTAACAAAGGCAGCTGTGTCTGAGCCCAACCCTTCTAAACGGTGACCTTTAGTGCCAACTTCCCCTCTAACTGGACAGCCTCTTCTGTCCCAAGTCTCCAGAGAGAAATCAGGCCTGATGAGGGGGAATTCCTGGAACCTGGACCCCAGCCTTGGTGGGGGAGCCTCTGGAATGCATGGGGCGGGTCCTAGCTGTTAGGGACATTTCCAAGCTGTTAGTTGCTGTTTAAAATAGAAATAAAATTGAAGACTAAAGACCTA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 50510, GC content failed 2244, low tm 14697, high tm 24074, high any compl 3, high end compl 30, high repeat similarity 17, long poly-x seq 96, ok 9349
PRIMER_RIGHT_EXPLAIN=considered 50486, GC content failed 2145, low tm 14382, high tm 24309, high end compl 3, high repeat similarity 3, long poly-x seq 101, ok 9543
PRIMER_PAIR_EXPLAIN=considered 661, unacceptable product size 598, high end compl 9, ok 54
PRIMER_PAIR_PENALTY=0.0114
PRIMER_LEFT_PENALTY=0.001065
PRIMER_RIGHT_PENALTY=0.010384
PRIMER_LEFT_SEQUENCE=AGACTAACAAAGGCAGCTGTGTC
PRIMER_RIGHT_SEQUENCE=TAACAGCTTGGAAATGTCCCTAA
PRIMER_LEFT=4101,23
PRIMER_RIGHT=4320,23
PRIMER_LEFT_TM=59.999
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.0153
PRIMER_LEFT_1_PENALTY=0.004943
PRIMER_RIGHT_1_PENALTY=0.010384
PRIMER_LEFT_1_SEQUENCE=CTTCTCCATACCCACAATCTGAG
PRIMER_RIGHT_1_SEQUENCE=TAACAGCTTGGAAATGTCCCTAA
PRIMER_LEFT_1=4051,23
PRIMER_RIGHT_1=4320,23
PRIMER_LEFT_1_TM=60.005
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, LTR1 LTR from human endogenous retrovirus-like sequence (HUERS-P2).
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.0653
PRIMER_LEFT_2_PENALTY=0.064245
PRIMER_RIGHT_2_PENALTY=0.001065
PRIMER_LEFT_2_SEQUENCE=CTCAATGAGACACAGAGGCTTTT
PRIMER_RIGHT_2_SEQUENCE=AGACACAGCTGCCTTTGTTAGTC
PRIMER_LEFT_2=3240,23
PRIMER_RIGHT_2=4124,23
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=59.999
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=885
PRIMER_PAIR_PENALTY_3=0.0692
PRIMER_LEFT_3_PENALTY=0.064245
PRIMER_RIGHT_3_PENALTY=0.004943
PRIMER_LEFT_3_SEQUENCE=CTCAATGAGACACAGAGGCTTTT
PRIMER_RIGHT_3_SEQUENCE=CTCAGATTGTGGGTATGGAGAAG
PRIMER_LEFT_3=3240,23
PRIMER_RIGHT_3=4073,23
PRIMER_LEFT_3_TM=59.936
PRIMER_RIGHT_3_TM=60.005
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=834
PRIMER_PAIR_PENALTY_4=0.0737
PRIMER_LEFT_4_PENALTY=0.064245
PRIMER_RIGHT_4_PENALTY=0.009437
PRIMER_LEFT_4_SEQUENCE=CTCAATGAGACACAGAGGCTTTT
PRIMER_RIGHT_4_SEQUENCE=TGGATATTTAAACCCCAAGTGTG
PRIMER_LEFT_4=3240,23
PRIMER_RIGHT_4=3747,23
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.991
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MSTc consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=508
PRIMER_PAIR_PENALTY_5=0.0835
PRIMER_LEFT_5_PENALTY=0.074111
PRIMER_RIGHT_5_PENALTY=0.009437
PRIMER_LEFT_5_SEQUENCE=ATCTCATGTACCAGTGCTGGAGT
PRIMER_RIGHT_5_SEQUENCE=TGGATATTTAAACCCCAAGTGTG
PRIMER_LEFT_5=2917,23
PRIMER_RIGHT_5=3747,23
PRIMER_LEFT_5_TM=60.074
PRIMER_RIGHT_5_TM=59.991
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MER9 a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MSTc consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=7.6000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=831
PRIMER_PAIR_PENALTY_6=0.0909
PRIMER_LEFT_6_PENALTY=0.061028
PRIMER_RIGHT_6_PENALTY=0.029845
PRIMER_LEFT_6_SEQUENCE=CAGTGGGGACTACTATCGTCAAG
PRIMER_RIGHT_6_SEQUENCE=TCAGCTCTCTCGATGTTGATGTA
PRIMER_LEFT_6=2672,23
PRIMER_RIGHT_6=3061,23
PRIMER_LEFT_6_TM=60.061
PRIMER_RIGHT_6_TM=60.030
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=390
PRIMER_PAIR_PENALTY_7=0.1040
PRIMER_LEFT_7_PENALTY=0.074111
PRIMER_RIGHT_7_PENALTY=0.029845
PRIMER_LEFT_7_SEQUENCE=ATCTCATGTACCAGTGCTGGAGT
PRIMER_RIGHT_7_SEQUENCE=TCAGCTCTCTCGATGTTGATGTA
PRIMER_LEFT_7=2917,23
PRIMER_RIGHT_7=3061,23
PRIMER_LEFT_7_TM=60.074
PRIMER_RIGHT_7_TM=60.030
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER9 a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.6000
PRIMER_RIGHT_7_END_STABILITY=5.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=145
PRIMER_PAIR_PENALTY_8=0.1183
PRIMER_LEFT_8_PENALTY=0.088469
PRIMER_RIGHT_8_PENALTY=0.029845
PRIMER_LEFT_8_SEQUENCE=GATTTACAACTACCTCATTGGCG
PRIMER_RIGHT_8_SEQUENCE=TCAGCTCTCTCGATGTTGATGTA
PRIMER_LEFT_8=2849,23
PRIMER_RIGHT_8=3061,23
PRIMER_LEFT_8_TM=59.912
PRIMER_RIGHT_8_TM=60.030
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=11.7000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=213
PRIMER_PAIR_PENALTY_9=0.1231
PRIMER_LEFT_9_PENALTY=0.122060
PRIMER_RIGHT_9_PENALTY=0.001065
PRIMER_LEFT_9_SEQUENCE=GTAAGGGGTTGGTATCTCAGGTC
PRIMER_RIGHT_9_SEQUENCE=AGACACAGCTGCCTTTGTTAGTC
PRIMER_LEFT_9=3809,23
PRIMER_RIGHT_9=4124,23
PRIMER_LEFT_9_TM=60.122
PRIMER_RIGHT_9_TM=59.999
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.6000
PRIMER_RIGHT_9_END_STABILITY=5.4000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=316
PRIMER_PAIR_PENALTY_10=0.1231
PRIMER_LEFT_10_PENALTY=0.122060
PRIMER_RIGHT_10_PENALTY=0.001065
PRIMER_LEFT_10_SEQUENCE=GTAAGGGGTTGGTATCTCAGGTC
PRIMER_RIGHT_10_SEQUENCE=GACACAGCTGCCTTTGTTAGTCT
PRIMER_LEFT_10=3809,23
PRIMER_RIGHT_10=4123,23
PRIMER_LEFT_10_TM=60.122
PRIMER_RIGHT_10_TM=59.999
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=315
PRIMER_PAIR_PENALTY_11=0.1257
PRIMER_LEFT_11_PENALTY=0.124598
PRIMER_RIGHT_11_PENALTY=0.001065
PRIMER_LEFT_11_SEQUENCE=CTCAGGTCTGAATCTTCACCATC
PRIMER_RIGHT_11_SEQUENCE=AGACACAGCTGCCTTTGTTAGTC
PRIMER_LEFT_11=3824,23
PRIMER_RIGHT_11=4124,23
PRIMER_LEFT_11_TM=60.125
PRIMER_RIGHT_11_TM=59.999
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, reverse MER4C a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=5.4000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=301
PRIMER_PAIR_PENALTY_12=0.1257
PRIMER_LEFT_12_PENALTY=0.124598
PRIMER_RIGHT_12_PENALTY=0.001065
PRIMER_LEFT_12_SEQUENCE=CTCAGGTCTGAATCTTCACCATC
PRIMER_RIGHT_12_SEQUENCE=GACACAGCTGCCTTTGTTAGTCT
PRIMER_LEFT_12=3824,23
PRIMER_RIGHT_12=4123,23
PRIMER_LEFT_12_TM=60.125
PRIMER_RIGHT_12_TM=59.999
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.1000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=300
PRIMER_PAIR_PENALTY_13=0.1270
PRIMER_LEFT_13_PENALTY=0.122060
PRIMER_RIGHT_13_PENALTY=0.004943
PRIMER_LEFT_13_SEQUENCE=GTAAGGGGTTGGTATCTCAGGTC
PRIMER_RIGHT_13_SEQUENCE=CTCAGATTGTGGGTATGGAGAAG
PRIMER_LEFT_13=3809,23
PRIMER_RIGHT_13=4073,23
PRIMER_LEFT_13_TM=60.122
PRIMER_RIGHT_13_TM=60.005
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.6000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=265
PRIMER_PAIR_PENALTY_14=0.1324
PRIMER_LEFT_14_PENALTY=0.122060
PRIMER_RIGHT_14_PENALTY=0.010384
PRIMER_LEFT_14_SEQUENCE=GTAAGGGGTTGGTATCTCAGGTC
PRIMER_RIGHT_14_SEQUENCE=TAACAGCTTGGAAATGTCCCTAA
PRIMER_LEFT_14=3809,23
PRIMER_RIGHT_14=4320,23
PRIMER_LEFT_14_TM=60.122
PRIMER_RIGHT_14_TM=60.010
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.5000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=512
PRIMER_PAIR_PENALTY_15=0.1350
PRIMER_LEFT_15_PENALTY=0.124598
PRIMER_RIGHT_15_PENALTY=0.010384
PRIMER_LEFT_15_SEQUENCE=CTCAGGTCTGAATCTTCACCATC
PRIMER_RIGHT_15_SEQUENCE=TAACAGCTTGGAAATGTCCCTAA
PRIMER_LEFT_15=3824,23
PRIMER_RIGHT_15=4320,23
PRIMER_LEFT_15_TM=60.125
PRIMER_RIGHT_15_TM=60.010
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=7.5000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=497
PRIMER_PAIR_PENALTY_16=0.1359
PRIMER_LEFT_16_PENALTY=0.074918
PRIMER_RIGHT_16_PENALTY=0.061028
PRIMER_LEFT_16_SEQUENCE=CTACAAACTGTCCTGGGTTCAAG
PRIMER_RIGHT_16_SEQUENCE=CTTGACGATAGTAGTCCCCACTG
PRIMER_LEFT_16=1700,23
PRIMER_RIGHT_16=2694,23
PRIMER_LEFT_16_TM=60.075
PRIMER_RIGHT_16_TM=60.061
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=6.7000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=995
PRIMER_PAIR_PENALTY_17=0.1384
PRIMER_LEFT_17_PENALTY=0.064245
PRIMER_RIGHT_17_PENALTY=0.074111
PRIMER_LEFT_17_SEQUENCE=ACAGTGGGGACTACTATCGTCAA
PRIMER_RIGHT_17_SEQUENCE=ACTCCAGCACTGGTACATGAGAT
PRIMER_LEFT_17=2671,23
PRIMER_RIGHT_17=2939,23
PRIMER_LEFT_17_TM=59.936
PRIMER_RIGHT_17_TM=60.074
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.7000
PRIMER_RIGHT_17_END_STABILITY=6.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=269
PRIMER_PAIR_PENALTY_18=0.1392
PRIMER_LEFT_18_PENALTY=0.074918
PRIMER_RIGHT_18_PENALTY=0.064245
PRIMER_LEFT_18_SEQUENCE=CTACAAACTGTCCTGGGTTCAAG
PRIMER_RIGHT_18_SEQUENCE=TTGACGATAGTAGTCCCCACTGT
PRIMER_LEFT_18=1700,23
PRIMER_RIGHT_18=2693,23
PRIMER_LEFT_18_TM=60.075
PRIMER_RIGHT_18_TM=59.936
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_18_END_STABILITY=7.0000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=994
PRIMER_PAIR_PENALTY_19=0.1495
PRIMER_LEFT_19_PENALTY=0.061028
PRIMER_RIGHT_19_PENALTY=0.088469
PRIMER_LEFT_19_SEQUENCE=CAGTGGGGACTACTATCGTCAAG
PRIMER_RIGHT_19_SEQUENCE=CGCCAATGAGGTAGTTGTAAATC
PRIMER_LEFT_19=2672,23
PRIMER_RIGHT_19=2871,23
PRIMER_LEFT_19_TM=60.061
PRIMER_RIGHT_19_TM=59.912
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.9000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=200
=