PRIMER_SEQUENCE_ID=NM_006343
SEQUENCE=GAATTCGTGTCTCGGCACTCACTCCCGGCCGCCCGGACAGGGAGCTTTCGCTGGCGCGCTTGGCCGGCGACAGGACAGGTTCGGGACGTCCATCTGTCCATCCGTCCGGAGAGAAATTACAGATCCGCAGCCCCGGGATGGGGCCGGCCCCGCTGCCGCTGCTGCTGGGCCTCTTCCTCCCCGCGCTCTGGCGTAGAGCTATCACTGAGGCAAGGGAAGAAGCCAAGCCTTACCCGCTATTCCCGGGACCTTTTCCAGGGAGCCTGCAAACTGACCACACACCGCTGTTATCCCTTCCTCACGCCAGTGGGTACCAGCCTGCCTTGATGTTTTCACCAACCCAGCCTGGAAGACCACATACAGGAAACGTAGCCATTCCCCAGGTGACCTCTGTCGAATCAAAGCCCCTACCGCCTCTTGCCTTCAAACACACAGTTGGACACATAATACTTTCTGAACATAAAGGTGTCAAATTTAATTGCTCAATCAATGTACCTAATATATACCAGGACACCACAATTTCTTGGTGGAAAGATGGGAAGGAATTGCTTGGGGGACATCATCGAATTACACAGTTTTATCCAGATGATGAAGTTACAGCAATAATCGCTTCCTTCAGCATAACCAGTGTGCAGCGTTCAGACAATGGGTCGTATATCTGTAAGATGAAAATAAACAATGAAGAGATCGTGTCTGATCCCATCTACATCGAAGTACAAGGACTTCCTCACTTTACTAAGCAGCCTGAGAGCATGAATGTCACCAGAAACACAGCCTTCAACCTCACCTGTCAGGCTGTGGGCCCGCCTGAGCCCGTCAACATTTTCTGGGTTCAAAACAGTAGCCGTGTTAACGAACAGCCTGAAAAATCCCCCGGCGTGCTAACTGTTCCAGGCCTGACGGAGATGGCGGTCTTCAGTTGTGAGGCCCACAATGACAAAGGGCTGACCGTGTCCCAGGGAGTGCAGATCAACATCAAAGCAATTCCCTCCCCACCAACTGAAGTCAGCATCCGTAACAGCACTGCACACAGCATTCTGATCTCCTGGGTTCCTGGTTTTGATGGATACTCCCCGTTCAGGAATTGCAGCATTCAGGTCAAGGAAGCTGATCCGCTGGGTAATGGCTCAGTCATGATTTTTAACACCTCTGCCTTACCACATCTGTACCAAATCAAGCAGCTGCAAGCCCTGGCTAATTACAGCATTGGTGTTTCCTGCATGAATGAAATAGGCTGGTCTGCAGTGAGCCCTTGGATTCTAGCAAGCACGACTGAAGGAGCCCCATCAGTAGCACCTTTAAATGTCACTGTGTTTCTGAATGAATCTAGTGATAATGTGGACATCAGATGGATGAAGCCTCCGACTAAGCAGCAGGATGGAGAACTGGTGGGCTACCGGATATCCCACGTGTGGCAGAGTGCAGGGATTTCCAAAGAGCTCTTGGAGGAAGTTGGCCAGAATGGCAGCCGAGCTCGGATCTCTGTTCAAGTCCACAATGCTACGTGCACAGTGAGGATTGCAGCCGTCACCAGAGGGGGAGTTGGGCCCTTCAGTGATCCAGTGAAAATATTTATCCCTGCACACGGTTGGGTAGATTATGCCCCCTCTTCAACTCCGGCGCCTGGCAACGCAGATCCTGTGCTCATCATCTTTGGCTGCTTTTGTGGATTTATTTTGATTGGGTTGATTTTATACATCTCCTTGGCCATCAGAAAAAGAGTCCAGGAGACAAAGTTTGGGAATGCATTCACAGAGGAGGATTCTGAATTAGTGGTGAATTATATAGCAAAGAAATCCTTCTGTCGGCGAGCCATTGAACTTACCTTACATAGCTTGGGAGTCAGTGAGGAACTACAAAATAAACTAGAAGATGTTGTGATTGACAGGAATCTTCTAATTCTTGGAAAAATTCTGGGTGAAGGAGAGTTTGGGTCTGTAATGGAAGGAAATCTTAAGCAGGAAGATGGGACCTCTCTGAAAGTGGCAGTGAAGACCATGAAGTTGGACAACTCTTCACATCGGGAGATCGAGGAGTTTCTCAGTGAGGCAGCGTGCATGAAAGACTTCAGCCACCCAAATGTCATTCGACTTCTAGGTGTGTGTATAGAAATGAGCTCTCAAGGCATCCCAAAGCCCATGGTAATTTTACCCTTCATGAAATACGGGGACCTGCATACTTACTTACTTTATTCCCGATTGGAGACAGGACCAAAGCATATTCCTCTGCAGACACTATTGAAGTTCATGGTGGATATTGCCCTGGGAATGGAGTATCTGAGCAACAGGAATTTTCTTCATCGAGATTTAGCTGCTCGAAACTGCATGTTGCGAGATGACATGACTGTCTGTGTTGCGGACTTCGGCCTCTCTAAGAAGATTTACAGTGGCGATTATTACCGCCAAGGCCGCATTGCTAAGATGCCTGTTAAATGGATCGCCATAGAAAGTCTTGCAGACCGAGTCTACACAAGTAAAAGTGATGTGTGGGCATTTGGCGTGACCATGTGGGAAATACGTACGCGGGGAATGACTCCCTATCCTGGGGTCCAGAACCATGAGATGTATGACTATCTTCTCCATGGCCACAGGTTGAAGCAGCCCGAAGACTGCCTGGATGAACTGTATGAAATAATGTACTCTTGCTGGAGAACCGATCCCTTAGACCGCCCCACCTTTTCAGTATTGAGGCTGCAGCTAGAAAAACTCTTAGAAAGTTTGCCTGACGTTCGGAACCAAGCAGACGTTATTTACGTCAATACACAGTTGCTGGAGAGCTCTGAGGGCCTGGCCCAGGGCCCCACCCTTGCTCCACTGGACTTGAACATCGACCCTGACTCTATAATTGCCTCCTGCACTCCCCGCGCTGCCATCAGTGTGGTCACAGCAGAAGTTCATGACAGCAAACCTCATGAAGGACGGTACATCCTGAATGGGGGCAGTGAGGAATGGGAAGATCTGACTTCTGCCCCCTCTGCTGCAGTCACAGCTGAAAAGAACAGTGTTTTACCGGGGGAGAGACTTGTTAGGAATGGGGTCTCCTGGTCCCATTCGAGCATGCTGCCCTTGGGAAGCTCATTGCCCGATGAACTTTTGTTTGCTGACGACTCCTCAGAAGGCTCAGAAGTCCTGATGTGAGGAGAGGTGCGGGGAGACATTCCAAAAATCAAGCCAATTCTTCTGCTGTAGGAGAATCCAATTGTACCTGATGTTTTTGGTATTTGTCTTCCTTACCAAGTGAACTCCATGGCCCCAAAGCACCAGATGAATGTTGTTAAGGAAGCTGTCATTAAAAATACATAATATATATTTATTTAAAGAGAAAAAATATGTGTATATCATGAAAAAGACAAGGATATTTTAATAAAACATTACTTATTTCATTTCACTTATCTTGCATATCTTAAAATTAAGCTTCAGCTGCTCCTTGATATTAACCTTTGTACAGAGTTGAAGTTGTTTTTTCAACTTCTTTTCTTTTTCATTACTATTAAATGTAAAAATATTTGTAAAATGAAATGCCATATTTGACTTGGCTTCTGGTCTTGATGTATTTGATAAGAATGATTAATTTTCTGATATGGCTTCCATAATAAAATTGAAATAGGA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 41846, GC content failed 1751, low tm 17382, high tm 13737, high any compl 1, high end compl 22, high repeat similarity 19, long poly-x seq 35, ok 8899
PRIMER_RIGHT_EXPLAIN=considered 41867, GC content failed 1828, low tm 17852, high tm 13167, high end compl 9, high repeat similarity 11, long poly-x seq 29, ok 8971
PRIMER_PAIR_EXPLAIN=considered 373, unacceptable product size 331, ok 42
PRIMER_PAIR_PENALTY=0.0022
PRIMER_LEFT_PENALTY=0.000148
PRIMER_RIGHT_PENALTY=0.002035
PRIMER_LEFT_SEQUENCE=TAGAAATGAGCTCTCAAGGCATC
PRIMER_RIGHT_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT=2106,23
PRIMER_RIGHT=2852,23
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=747
PRIMER_PAIR_PENALTY_1=0.0032
PRIMER_LEFT_1_PENALTY=0.000148
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=TAGAAATGAGCTCTCAAGGCATC
PRIMER_RIGHT_1_SEQUENCE=GCAGCTAAATCTCGATGAAGAAA
PRIMER_LEFT_1=2106,23
PRIMER_RIGHT_1=2313,23
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER44C Nonautonomous DNA transposon
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.0051
PRIMER_LEFT_2_PENALTY=0.003045
PRIMER_RIGHT_2_PENALTY=0.002035
PRIMER_LEFT_2_SEQUENCE=TTTCTTCATCGAGATTTAGCTGC
PRIMER_RIGHT_2_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT_2=2291,23
PRIMER_RIGHT_2=2852,23
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=562
PRIMER_PAIR_PENALTY_3=0.0098
PRIMER_LEFT_3_PENALTY=0.007752
PRIMER_RIGHT_3_PENALTY=0.002035
PRIMER_LEFT_3_SEQUENCE=CCAAATGTCATTCGACTTCTAGG
PRIMER_RIGHT_3_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT_3=2075,23
PRIMER_RIGHT_3=2852,23
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=778
PRIMER_PAIR_PENALTY_4=0.0108
PRIMER_LEFT_4_PENALTY=0.007752
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=CCAAATGTCATTCGACTTCTAGG
PRIMER_RIGHT_4_SEQUENCE=GCAGCTAAATCTCGATGAAGAAA
PRIMER_LEFT_4=2075,23
PRIMER_RIGHT_4=2313,23
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER44C Nonautonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
PRIMER_PAIR_PENALTY_5=0.0144
PRIMER_LEFT_5_PENALTY=0.012411
PRIMER_RIGHT_5_PENALTY=0.002035
PRIMER_LEFT_5_SEQUENCE=TGCAGACCGAGTCTACACAAGTA
PRIMER_RIGHT_5_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT_5=2452,23
PRIMER_RIGHT_5=2852,23
PRIMER_LEFT_5_TM=59.988
PRIMER_RIGHT_5_TM=59.998
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=5.7000
PRIMER_RIGHT_5_END_STABILITY=9.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=401
PRIMER_PAIR_PENALTY_6=0.0176
PRIMER_LEFT_6_PENALTY=0.015613
PRIMER_RIGHT_6_PENALTY=0.002035
PRIMER_LEFT_6_SEQUENCE=AATCTTAAGCAGGAAGATGGGAC
PRIMER_RIGHT_6_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT_6=1949,23
PRIMER_RIGHT_6=2852,23
PRIMER_LEFT_6_TM=59.984
PRIMER_RIGHT_6_TM=59.998
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.1000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=904
PRIMER_PAIR_PENALTY_7=0.0187
PRIMER_LEFT_7_PENALTY=0.015613
PRIMER_RIGHT_7_PENALTY=0.003045
PRIMER_LEFT_7_SEQUENCE=AATCTTAAGCAGGAAGATGGGAC
PRIMER_RIGHT_7_SEQUENCE=GCAGCTAAATCTCGATGAAGAAA
PRIMER_LEFT_7=1949,23
PRIMER_RIGHT_7=2313,23
PRIMER_LEFT_7_TM=59.984
PRIMER_RIGHT_7_TM=60.003
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER44C Nonautonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.1000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=365
PRIMER_PAIR_PENALTY_8=0.0203
PRIMER_LEFT_8_PENALTY=0.018314
PRIMER_RIGHT_8_PENALTY=0.002035
PRIMER_LEFT_8_SEQUENCE=AATGGAAGGAAATCTTAAGCAGG
PRIMER_RIGHT_8_SEQUENCE=AGGAGGCAATTATAGAGTCAGGG
PRIMER_LEFT_8=1939,23
PRIMER_RIGHT_8=2852,23
PRIMER_LEFT_8_TM=59.982
PRIMER_RIGHT_8_TM=59.998
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=9.7000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=914
PRIMER_PAIR_PENALTY_9=0.0214
PRIMER_LEFT_9_PENALTY=0.018314
PRIMER_RIGHT_9_PENALTY=0.003045
PRIMER_LEFT_9_SEQUENCE=AATGGAAGGAAATCTTAAGCAGG
PRIMER_RIGHT_9_SEQUENCE=GCAGCTAAATCTCGATGAAGAAA
PRIMER_LEFT_9=1939,23
PRIMER_RIGHT_9=2313,23
PRIMER_LEFT_9_TM=59.982
PRIMER_RIGHT_9_TM=60.003
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MSTAR MSTa-Mammalian LTR internal retrotransposon sequence - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER44C Nonautonomous DNA transposon
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=375
PRIMER_PAIR_PENALTY_10=0.0539
PRIMER_LEFT_10_PENALTY=0.000148
PRIMER_RIGHT_10_PENALTY=0.053769
PRIMER_LEFT_10_SEQUENCE=TAGAAATGAGCTCTCAAGGCATC
PRIMER_RIGHT_10_SEQUENCE=ATGAGGTTTGCTGTCATGAACTT
PRIMER_LEFT_10=2106,23
PRIMER_RIGHT_10=2911,23
PRIMER_LEFT_10_TM=60.000
PRIMER_RIGHT_10_TM=60.054
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=806
PRIMER_PAIR_PENALTY_11=0.0568
PRIMER_LEFT_11_PENALTY=0.003045
PRIMER_RIGHT_11_PENALTY=0.053769
PRIMER_LEFT_11_SEQUENCE=TTTCTTCATCGAGATTTAGCTGC
PRIMER_RIGHT_11_SEQUENCE=ATGAGGTTTGCTGTCATGAACTT
PRIMER_LEFT_11=2291,23
PRIMER_RIGHT_11=2911,23
PRIMER_LEFT_11_TM=60.003
PRIMER_RIGHT_11_TM=60.054
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=621
PRIMER_PAIR_PENALTY_12=0.0615
PRIMER_LEFT_12_PENALTY=0.007752
PRIMER_RIGHT_12_PENALTY=0.053769
PRIMER_LEFT_12_SEQUENCE=CCAAATGTCATTCGACTTCTAGG
PRIMER_RIGHT_12_SEQUENCE=ATGAGGTTTGCTGTCATGAACTT
PRIMER_LEFT_12=2075,23
PRIMER_RIGHT_12=2911,23
PRIMER_LEFT_12_TM=60.008
PRIMER_RIGHT_12_TM=60.054
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=3.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=837
PRIMER_PAIR_PENALTY_13=0.0617
PRIMER_LEFT_13_PENALTY=0.058607
PRIMER_RIGHT_13_PENALTY=0.003045
PRIMER_LEFT_13_SEQUENCE=TTTGTGGATTTATTTTGATTGGG
PRIMER_RIGHT_13_SEQUENCE=GCAGCTAAATCTCGATGAAGAAA
PRIMER_LEFT_13=1662,23
PRIMER_RIGHT_13=2313,23
PRIMER_LEFT_13_TM=59.941
PRIMER_RIGHT_13_TM=60.003
PRIMER_LEFT_13_GC_PERCENT=30.435
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER44C Nonautonomous DNA transposon
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=10.0000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=652
PRIMER_PAIR_PENALTY_14=0.0617
PRIMER_LEFT_14_PENALTY=0.043423
PRIMER_RIGHT_14_PENALTY=0.018314
PRIMER_LEFT_14_SEQUENCE=TTTTTAACACCTCTGCCTTACCA
PRIMER_RIGHT_14_SEQUENCE=CCTGCTTAAGATTTCCTTCCATT
PRIMER_LEFT_14=1137,23
PRIMER_RIGHT_14=1961,23
PRIMER_LEFT_14_TM=60.043
PRIMER_RIGHT_14_TM=59.982
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER31 a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=8.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=825
PRIMER_PAIR_PENALTY_15=0.0662
PRIMER_LEFT_15_PENALTY=0.012411
PRIMER_RIGHT_15_PENALTY=0.053769
PRIMER_LEFT_15_SEQUENCE=TGCAGACCGAGTCTACACAAGTA
PRIMER_RIGHT_15_SEQUENCE=ATGAGGTTTGCTGTCATGAACTT
PRIMER_LEFT_15=2452,23
PRIMER_RIGHT_15=2911,23
PRIMER_LEFT_15_TM=59.988
PRIMER_RIGHT_15_TM=60.054
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=8.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=5.7000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=460
PRIMER_PAIR_PENALTY_16=0.0680
PRIMER_LEFT_16_PENALTY=0.000148
PRIMER_RIGHT_16_PENALTY=0.067837
PRIMER_LEFT_16_SEQUENCE=TAGAAATGAGCTCTCAAGGCATC
PRIMER_RIGHT_16_SEQUENCE=GACTCGGTCTGCAAGACTTTCTA
PRIMER_LEFT_16=2106,23
PRIMER_RIGHT_16=2464,23
PRIMER_LEFT_16_TM=60.000
PRIMER_RIGHT_16_TM=60.068
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=6.0000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=359
PRIMER_PAIR_PENALTY_17=0.0694
PRIMER_LEFT_17_PENALTY=0.015613
PRIMER_RIGHT_17_PENALTY=0.053769
PRIMER_LEFT_17_SEQUENCE=AATCTTAAGCAGGAAGATGGGAC
PRIMER_RIGHT_17_SEQUENCE=ATGAGGTTTGCTGTCATGAACTT
PRIMER_LEFT_17=1949,23
PRIMER_RIGHT_17=2911,23
PRIMER_LEFT_17_TM=59.984
PRIMER_RIGHT_17_TM=60.054
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.1000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=963
PRIMER_PAIR_PENALTY_18=0.0695
PRIMER_LEFT_18_PENALTY=0.000148
PRIMER_RIGHT_18_PENALTY=0.069319
PRIMER_LEFT_18_SEQUENCE=TAGAAATGAGCTCTCAAGGCATC
PRIMER_RIGHT_18_SEQUENCE=CGTAAATAACGTCTGCTTGGTTC
PRIMER_LEFT_18=2106,23
PRIMER_RIGHT_18=2753,23
PRIMER_LEFT_18_TM=60.000
PRIMER_RIGHT_18_TM=60.069
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=648
PRIMER_PAIR_PENALTY_19=0.0709
PRIMER_LEFT_19_PENALTY=0.003045
PRIMER_RIGHT_19_PENALTY=0.067837
PRIMER_LEFT_19_SEQUENCE=TTTCTTCATCGAGATTTAGCTGC
PRIMER_RIGHT_19_SEQUENCE=GACTCGGTCTGCAAGACTTTCTA
PRIMER_LEFT_19=2291,23
PRIMER_RIGHT_19=2464,23
PRIMER_LEFT_19_TM=60.003
PRIMER_RIGHT_19_TM=60.068
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=6.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=174
=