PRIMER_SEQUENCE_ID=NM_013993
SEQUENCE=GGCTTAGGAAGTATTAACTGATCTCTGCCCTAGTTCTCATGTGTTAAATATGGATAGTAATAGTATCTACCTTATGAAGTGACTGTGAAGATAAAATTATGGATTCTGTTTAAGGGTTTAGGCCAGTGTCTGGCACAGGGGAAGCATTCTAAAAATATAGCTGATGCTGTTAAACAATGACTGTTGTTGTTGTTTTACTGTTATTATCCCCAAAGCGGCCCATTCTGTCTGTTGCTGTCAGCTATGACTCAGTCCCCTGATTAACTTACGCACCACCCATTTTATCCCCTGCAGAGATGCTGCCCCCACCCCCTTAGGCCCGAGGGATCAGGAGCTATGGGACCAGAGGCCCTGTCATCTTTACTGCTGCTGCTCTTGGTGGCAAGTGGAGATGCTGACATGAAGGGACATTTTGATCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGGACCATCCCAGACAGTGACATCTCTGCTTCCAGCTCCTGGTCAGATTCCACTGCCGCCCGCCACAGCAGGTTGGAGAGCAGTGACGGGGATGGGGCCTGGTGCCCCGCAGGGTCGGTGTTTCCCAAGGAGGAGGAGTACTTGCAGGTGGATCTACAACGACTGCACCTGGTGGCTCTGGTGGGCACCCAGGGACGGCATGCCGGGGGCCTGGGCAAGGAGTTCTCCCGGAGCTACCGGCTGCGTTACTCCCGGGATGGTCGCCGCTGGATGGGCTGGAAGGACCGCTGGGGTCAGGAGGTGATCTCAGGCAATGAGGACCCTGAGGGAGTGGTGCTGAAGGACCTTGGGCCCCCCATGGTTGCCCGACTGGTTCGCTTCTACCCCCGGGCTGACCGGGTCATGAGCGTCTGTCTGCGGGTAGAGCTCTATGGCTGCCTCTGGAGGGATGGACTCCTGTCTTACACCGCCCCTGTGGGGCAGACAATGTATTTATCTGAGGCCGTGTACCTCAACGACTCCACCTATGACGGACATACCGTGGGCGGACTGCAGTATGGGGGTCTGGGCCAGCTGGCAGATGGTGTGGTGGGGCTGGATGACTTTAGGAAGAGTCAGGAGCTGCGGGTCTGGCCAGGCTATGACTATGTGGGATGGAGCAACCACAGCTTCTCCAGTGGCTATGTGGAGATGGAGTTTGAGTTTGACCGGCTGAGGGCCTTCCAGGCTATGCAGGTCCACTGTAACAACATGCACACGCTGGGAGCCCGTCTGCCTGGCGGGGTGGAATGTCGCTTCCGGCGTGGCCCTGCCATGGCCTGGGAGGGGGAGCCCATGCGCCACAACCTAGGGGGCAACCTGGGGGACCCCAGAGCCCGGGCTGTCTCAGTGCCCCTTGGCGGCCGTGTGGCTCGCTTTCTGCAGTGCCGCTTCCTCTTTGCGGGGCCCTGGTTACTCTTCAGCGAAATCTCCTTCATCTCTGATGTGGTGAACAATTCCTCTCCGGCACTGGGAGGCACCTTCCCGCCAGCCCCCTGGTGGCCGCCTGGCCCACCTCCCACCAACTTCAGCAGCTTGGAGCTGGAGCCCAGAGGCCAGCAGCCCGTGGCCAAGGCCGAGGGGAGCCCGACCGCCATCCTCATCGGCTGCCTGGTGGCCATCATCCTGCTCCTGCTGCTCATCATTGCCCTCATGCTCTGGCGGCTGCACTGGCGCAGGCTCCTCAGCAAGGCTGAACGGAGGGTGTTGGAAGAGGAGCTGACGGTTCACCTCTCTGTCCCTGGGGACACTATCCTCATCAACAACCGCCCAGGTCCTAGAGAGCCACCCCCGTACCAGGAGCCCCGGCCTCGTGGGAATCCGCCCCACTCCGCTCCCTGTGTCCCCAATGGCTCTGCGTTGCTGCTCTCCAATCCAGCCTACCGCCTCCTTCTGGCCACTTACGCCCGTCCCCCTCGAGGCCCGGGCCCCCCCACACCCGCCTGGGCCAAACCCACCAACACCCAGGCCTACAGTGGGGACTATATGGAGCCTGAGAAGCCAGGCGCCCCGCTTCTGCCCCCACCTCCCCAGAACAGCGTCCCCCATTATGCCGAGGCTGACATTGTTACCCTGCAGGGCGTCACCGGGGGCAACACCTATGCTGTGCCTGCACTGCCCCCAGGGGCAGTCGGGGATGGGCCCCCCAGAGTGGATTTCCCTCGATCTCGACTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAGTTTGGGGAGGTGCACCTGTGTGAGGTCGACAGCCCTCAAGATCTGGTTAGTCTTGATTTCCCCCTTAATGTGCGTAAGGGACACCCTTTGCTGGTAGCTGTCAAGATCTTACGGCCAGATGCCACCAAGAATGCCAGGAATGATTTCCTGAAAGAGGTGAAGATCATGTCGAGGCTCAAGGACCCAAACATCATTCGGCTGCTGGGCGTGTGTGTGCAGGACGACCCCCTCTGCATGATTACTGACTACATGGAGAACGGCGACCTCAACCAGTTCCTCAGTGCCCACCAGCTGGAGGACAAGGCAGCCGAGGGGGCCCCTGGGGACGGGCAGGCTGCGCAGGGGCCCACCATCAGCTACCCAATGCTGCTGCATGTGGCAGCCCAGATCGCCTCCGGCATGCGCTATCTGGCCACACTCAACTTTGTACATCGGGACCTGGCCACGCGGAACTGCCTAGTTGGGGAAAATTTCACCATCAAAATCGCAGACTTTGGCATGAGCCGGAACCTCTATGCTGGGGACTATTACCGTGTGCAGGGCCGGGCAGTGCTGCCCATCCGCTGGATGGCCTGGGAGTGCATCCTCATGGGGAAGTTCACGACTGCGAGTGACGTGTGGGCCTTTGGTGTGACCCTGTGGGAGGTGCTGATGCTCTGTAGGGCCCAGCCCTTTGGGTCAGCTCACCGACGAGCAGGTCATCGAGAACGCGGGGGAGTTCTTCCGGGACCAGGGCCGGCAGTGTACCTGTCCCGGCCGCCTGCCTGCCCGCAGGGCCTATATGAGCTGATGCTTCGGTGCTGGAGCCGGGAGTCTGAGCAGCGACCACCCTTTTCCCAGCTGCATCGGTTCCTGGCAGAGGATGCACTCAACACGGTGTGAATCACACATCCAGCTGCCCCTCCCTCAGGGAGCGATCCAGGGGAAGCCAGTGACACTAAAACAAGAGGACACAATGGCACCTCTGCCCTTCCCCTCCCGACAGCCCATCACCTCTAATAGAGGCAGTGAGACTGCAGGTGGGCTGGGCCCACCCAGGGAGCTGATGCCCCTTCTCCCCTTCCTGGACACACTCTCATGTCCCCTTCCTGTTCTTCCTTCCTAGAAGCCCCCCTGTCGCCCACCCAGCTGGTCCTGTGGATGGGATCCTCTCCACCCTCCTCTAGCCATCCCTTGGGGAAGGGTGGGGAGAAATATAGGATAGACACTGGACATGGCCCATTGGAGCACCTGGGCCCCACTGGACAACACTGATTCCTGGAGAGGTGGCTGCGCCCCCAGCTTCTCTCTCCCTGTCACACACTGGACCCCACTGGCTGAGAATCTGGGGGTGAGGAGGACAAGAAGGAGAGGAAAATGTTTCCTTGTGCCTGCTCCTGTACTTGTCCTCAGCTTGGGCTTCTTCCTCCTCCATCACCTGAAACACTGGACCTGGGGGTAGCCCCGCCCCAGCCCTCAGTCACCCCCACTTCCCACTTGCAGTCTTGTAGCTAGAACTTCTCTAAGCCTATACGTTTCTGTGGAGTAAATATTGGGATTGGGGGGAAAGAGGGAGCAACGGCCCATAGCCTTGGGGTTGGACATCTCTAGTGTAGCTGCCACATTGATTTTTCTATAATCACTTGGGGTTTGTACATTTTTGGGGGGAGAGACACAGATTTTTACACTAATATATGGACCTAGCTTGAGGCAATTTTAATCCCCTGCACTAGGCAGGTAATAATAAAGGTTGAGTTTTCCACAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 45419, GC content failed 1796, low tm 11554, high tm 23888, high any compl 24, high end compl 59, high repeat similarity 72, long poly-x seq 109, ok 7917
PRIMER_RIGHT_EXPLAIN=considered 45338, GC content failed 1805, low tm 11257, high tm 23996, high any compl 1, high end compl 6, high repeat similarity 16, long poly-x seq 124, ok 8133
PRIMER_PAIR_EXPLAIN=considered 1179, unacceptable product size 1105, high any compl 6, high end compl 5, ok 63
PRIMER_PAIR_PENALTY=0.0916
PRIMER_LEFT_PENALTY=0.048411
PRIMER_RIGHT_PENALTY=0.043236
PRIMER_LEFT_SEQUENCE=CTAAAACAAGAGGACACAATGGC
PRIMER_RIGHT_SEQUENCE=CCAATCCCAATATTTACTCCACA
PRIMER_LEFT=3133,23
PRIMER_RIGHT=3736,23
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=59.957
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=604
PRIMER_PAIR_PENALTY_1=0.2062
PRIMER_LEFT_1_PENALTY=0.064245
PRIMER_RIGHT_1_PENALTY=0.141958
PRIMER_LEFT_1_SEQUENCE=TGTACCTCAACGACTCCACCTAT
PRIMER_RIGHT_1_SEQUENCE=ACTCTTCCTAAAGTCATCCAGCC
PRIMER_LEFT_1=958,23
PRIMER_RIGHT_1=1067,23
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=0.2062
PRIMER_LEFT_2_PENALTY=0.064245
PRIMER_RIGHT_2_PENALTY=0.141958
PRIMER_LEFT_2_SEQUENCE=GTACCTCAACGACTCCACCTATG
PRIMER_RIGHT_2_SEQUENCE=ACTCTTCCTAAAGTCATCCAGCC
PRIMER_LEFT_2=959,23
PRIMER_RIGHT_2=1067,23
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.2086
PRIMER_LEFT_3_PENALTY=0.064245
PRIMER_RIGHT_3_PENALTY=0.144318
PRIMER_LEFT_3_SEQUENCE=TGTACCTCAACGACTCCACCTAT
PRIMER_RIGHT_3_SEQUENCE=CTCAAACTCCATCTCCACATAGC
PRIMER_LEFT_3=958,23
PRIMER_RIGHT_3=1154,23
PRIMER_LEFT_3_TM=59.936
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_3_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.2478
PRIMER_LEFT_4_PENALTY=0.199411
PRIMER_RIGHT_4_PENALTY=0.048411
PRIMER_LEFT_4_SEQUENCE=AGCCCTCAAGATCTGGTTAGTCT
PRIMER_RIGHT_4_SEQUENCE=GCCATTGTGTCCTCTTGTTTTAG
PRIMER_LEFT_4=2226,23
PRIMER_RIGHT_4=3155,23
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=930
PRIMER_PAIR_PENALTY_5=0.2552
PRIMER_LEFT_5_PENALTY=0.211969
PRIMER_RIGHT_5_PENALTY=0.043236
PRIMER_LEFT_5_SEQUENCE=GCCAGTGACACTAAAACAAGAGG
PRIMER_RIGHT_5_SEQUENCE=CCAATCCCAATATTTACTCCACA
PRIMER_LEFT_5=3123,23
PRIMER_RIGHT_5=3736,23
PRIMER_LEFT_5_TM=60.212
PRIMER_RIGHT_5_TM=59.957
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=614
PRIMER_PAIR_PENALTY_6=0.2649
PRIMER_LEFT_6_PENALTY=0.122991
PRIMER_RIGHT_6_PENALTY=0.141958
PRIMER_LEFT_6_SEQUENCE=GACAATGTATTTATCTGAGGCCG
PRIMER_RIGHT_6_SEQUENCE=ACTCTTCCTAAAGTCATCCAGCC
PRIMER_LEFT_6=935,23
PRIMER_RIGHT_6=1067,23
PRIMER_LEFT_6_TM=59.877
PRIMER_RIGHT_6_TM=60.142
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=12.9000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=133
PRIMER_PAIR_PENALTY_7=0.2673
PRIMER_LEFT_7_PENALTY=0.122991
PRIMER_RIGHT_7_PENALTY=0.144318
PRIMER_LEFT_7_SEQUENCE=GACAATGTATTTATCTGAGGCCG
PRIMER_RIGHT_7_SEQUENCE=CTCAAACTCCATCTCCACATAGC
PRIMER_LEFT_7=935,23
PRIMER_RIGHT_7=1154,23
PRIMER_LEFT_7_TM=59.877
PRIMER_RIGHT_7_TM=60.144
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=2.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=12.9000
PRIMER_RIGHT_7_END_STABILITY=7.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=220
PRIMER_PAIR_PENALTY_8=0.2718
PRIMER_LEFT_8_PENALTY=0.043236
PRIMER_RIGHT_8_PENALTY=0.228597
PRIMER_LEFT_8_SEQUENCE=TGTGGAGTAAATATTGGGATTGG
PRIMER_RIGHT_8_SEQUENCE=GTAAAAATCTGTGTCTCTCCCCC
PRIMER_LEFT_8=3714,23
PRIMER_RIGHT_8=3860,23
PRIMER_LEFT_8_TM=59.957
PRIMER_RIGHT_8_TM=60.229
PRIMER_LEFT_8_GC_PERCENT=39.130
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MSTa consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=19.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=12.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=147
PRIMER_PAIR_PENALTY_9=0.2770
PRIMER_LEFT_9_PENALTY=0.048411
PRIMER_RIGHT_9_PENALTY=0.228597
PRIMER_LEFT_9_SEQUENCE=CTAAAACAAGAGGACACAATGGC
PRIMER_RIGHT_9_SEQUENCE=GTAAAAATCTGTGTCTCTCCCCC
PRIMER_LEFT_9=3133,23
PRIMER_RIGHT_9=3860,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=60.229
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=3.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MSTa consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=12.4000
PRIMER_PAIR_9_COMPL_ANY=7.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=728
PRIMER_PAIR_PENALTY_10=0.2863
PRIMER_LEFT_10_PENALTY=0.141958
PRIMER_RIGHT_10_PENALTY=0.144318
PRIMER_LEFT_10_SEQUENCE=GGCTGGATGACTTTAGGAAGAGT
PRIMER_RIGHT_10_SEQUENCE=CTCAAACTCCATCTCCACATAGC
PRIMER_LEFT_10=1045,23
PRIMER_RIGHT_10=1154,23
PRIMER_LEFT_10_TM=60.142
PRIMER_RIGHT_10_TM=60.144
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.1000
PRIMER_RIGHT_10_END_STABILITY=7.1000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=110
PRIMER_PAIR_PENALTY_11=0.2909
PRIMER_LEFT_11_PENALTY=0.226649
PRIMER_RIGHT_11_PENALTY=0.064245
PRIMER_LEFT_11_SEQUENCE=GTCATCTTTACTGCTGCTGCTCT
PRIMER_RIGHT_11_SEQUENCE=ATAGGTGGAGTCGTTGAGGTACA
PRIMER_LEFT_11=353,23
PRIMER_RIGHT_11=980,23
PRIMER_LEFT_11_TM=60.227
PRIMER_RIGHT_11_TM=59.936
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.9000
PRIMER_RIGHT_11_END_STABILITY=5.4000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=628
PRIMER_PAIR_PENALTY_12=0.2964
PRIMER_LEFT_12_PENALTY=0.232113
PRIMER_RIGHT_12_PENALTY=0.064245
PRIMER_LEFT_12_SEQUENCE=ATTCTGTTTAAGGGTTTAGGCCA
PRIMER_RIGHT_12_SEQUENCE=ATAGGTGGAGTCGTTGAGGTACA
PRIMER_LEFT_12=102,23
PRIMER_RIGHT_12=980,23
PRIMER_LEFT_12_TM=60.232
PRIMER_RIGHT_12_TM=59.936
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=11.2000
PRIMER_RIGHT_12_END_STABILITY=5.4000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=879
PRIMER_PAIR_PENALTY_13=0.3088
PRIMER_LEFT_13_PENALTY=0.260365
PRIMER_RIGHT_13_PENALTY=0.048411
PRIMER_LEFT_13_SEQUENCE=GGTTAGTCTTGATTTCCCCCTTA
PRIMER_RIGHT_13_SEQUENCE=GCCATTGTGTCCTCTTGTTTTAG
PRIMER_LEFT_13=2240,23
PRIMER_RIGHT_13=3155,23
PRIMER_LEFT_13_TM=59.740
PRIMER_RIGHT_13_TM=60.048
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.5000
PRIMER_RIGHT_13_END_STABILITY=6.3000
PRIMER_PAIR_13_COMPL_ANY=2.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=916
PRIMER_PAIR_PENALTY_14=0.3154
PRIMER_LEFT_14_PENALTY=0.064245
PRIMER_RIGHT_14_PENALTY=0.251135
PRIMER_LEFT_14_SEQUENCE=GTACCTCAACGACTCCACCTATG
PRIMER_RIGHT_14_SEQUENCE=GTTGTTGATGAGGATAGTGTCCC
PRIMER_LEFT_14=959,23
PRIMER_RIGHT_14=1757,23
PRIMER_LEFT_14_TM=59.936
PRIMER_RIGHT_14_TM=59.749
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.9000
PRIMER_RIGHT_14_END_STABILITY=9.1000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=799
PRIMER_PAIR_PENALTY_15=0.3154
PRIMER_LEFT_15_PENALTY=0.064245
PRIMER_RIGHT_15_PENALTY=0.251135
PRIMER_LEFT_15_SEQUENCE=TGTACCTCAACGACTCCACCTAT
PRIMER_RIGHT_15_SEQUENCE=GTTGTTGATGAGGATAGTGTCCC
PRIMER_LEFT_15=958,23
PRIMER_RIGHT_15=1757,23
PRIMER_LEFT_15_TM=59.936
PRIMER_RIGHT_15_TM=59.749
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.1000
PRIMER_RIGHT_15_END_STABILITY=9.1000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=800
PRIMER_PAIR_PENALTY_16=0.3182
PRIMER_LEFT_16_PENALTY=0.048411
PRIMER_RIGHT_16_PENALTY=0.269783
PRIMER_LEFT_16_SEQUENCE=CTAAAACAAGAGGACACAATGGC
PRIMER_RIGHT_16_SEQUENCE=ATCCTATATTTCTCCCCACCCTT
PRIMER_LEFT_16=3133,23
PRIMER_RIGHT_16=3397,23
PRIMER_LEFT_16_TM=60.048
PRIMER_RIGHT_16_TM=60.270
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.6000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=265
PRIMER_PAIR_PENALTY_17=0.3384
PRIMER_LEFT_17_PENALTY=0.274160
PRIMER_RIGHT_17_PENALTY=0.064245
PRIMER_LEFT_17_SEQUENCE=GTTTAAGGGTTTAGGCCAGTGTC
PRIMER_RIGHT_17_SEQUENCE=ATAGGTGGAGTCGTTGAGGTACA
PRIMER_LEFT_17=107,23
PRIMER_RIGHT_17=980,23
PRIMER_LEFT_17_TM=60.274
PRIMER_RIGHT_17_TM=59.936
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=5.4000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=874
PRIMER_PAIR_PENALTY_18=0.3686
PRIMER_LEFT_18_PENALTY=0.226649
PRIMER_RIGHT_18_PENALTY=0.141958
PRIMER_LEFT_18_SEQUENCE=GTCATCTTTACTGCTGCTGCTCT
PRIMER_RIGHT_18_SEQUENCE=ACTCTTCCTAAAGTCATCCAGCC
PRIMER_LEFT_18=353,23
PRIMER_RIGHT_18=1067,23
PRIMER_LEFT_18_TM=60.227
PRIMER_RIGHT_18_TM=60.142
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=9.7000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=715
PRIMER_PAIR_PENALTY_19=0.3710
PRIMER_LEFT_19_PENALTY=0.226649
PRIMER_RIGHT_19_PENALTY=0.144318
PRIMER_LEFT_19_SEQUENCE=GTCATCTTTACTGCTGCTGCTCT
PRIMER_RIGHT_19_SEQUENCE=CTCAAACTCCATCTCCACATAGC
PRIMER_LEFT_19=353,23
PRIMER_RIGHT_19=1154,23
PRIMER_LEFT_19_TM=60.227
PRIMER_RIGHT_19_TM=60.144
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse LTR8 region
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.9000
PRIMER_RIGHT_19_END_STABILITY=7.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=802
=