PRIMER_SEQUENCE_ID=NM_018423
SEQUENCE=ACCCAACCCTGTCATTATTTCCCCTCCGACTGGGCCGGTGCCATCGGAAACCGGAGTGACCAGAGGAGGGACACGCATCCAATTTCACTGGGCTGCAAGATATCAATAAGCCTTGCCTCTTTAAACTACTTATGTACAAATAAAAGTGATGGTGAGAACCTGGCTCAGGAAATGCAGTAGCAGGCCATATTGCATCCAAAGGAATTACTCACAGCTGTGCTGTGTGCATTCTCTGTGGGCCTAGCAGGGAAGGGGACAGCCCTGTGGCAATGGGCATGACACGGATGCTCCTGGAATGCAGTCTCAGTGACAAGTTGTGTGTCATCCAGGAGAAGCAGTATGAAGTGATTATCGTCCCAACTTTGTTGGTTACTATCTTCCTCATCCTTCTTGGGGTCATCCTGTGGCTTTTTATCAGAGAACAAAGAACTCAACAGCAGCGTTCTGGACCTCAAGGCATTGCCCCTGTTCCTCCACCTAGGGACCTAAGCTGGGAAGCAGGACATGGAGGAAATGTGGCTTTGCCACTTAAGGAGACATCCGTGGAAAACTTTCTGGGAGCTACCACACCTGCCCTGGCTAAGCTGCAGGTGCCGCGGGAGCAACTCTCTGAAGTTCTGGAGCAGATTTGCAGTGGTAGCTGTGGGCCCATCTTTCGAGCCAATATGAACACTGGGGACCCTTCTAAGCCCAAGAGTGTTATTCTCAAGGCTTTAAAAGAACCAGCTGGGCTCCATGAGGTACAAGATTTCTTAGGGCGAATCCAATTCCATCAATACCTGGGGAAACACAAAAACCTGGTGCAGCTGGAAGGCTGCTGCACTGAAAAGCTGCCACTCTATATGGTGTTGGAGGATGTGGCCCAGGGGGACCTGCTCGGCTTTCTCTGGACCTGTCGGCGGGATGTGATGACTATGGATGGTCTTCTCTATGATCTCACAGAAAAACAAGTATATCACATCGGAAAGCAGGTCCTTTTGGCGCTGGAATTCCTGCAGGAGAAGCATTTGTTCCATGGGGATGTGGCAGCCAGGAATATTCTGATGCAAAGTGATCTCACTGCTAAGCTCTGTGGATTAGGCCTGGCTTATGAAGTTTACACCCGAGGGGCCATCTCCTCTACTCAAACCATACCTCTCAAGTGGCTTGCCCCAGAACGGCTTCTCCTGAGACCTGCTAGCATCAGAGCAGATGTCTGGTCTTTTGGGATCCTGCTCTATGAGATGGTGACTCTAGGAGCACCACCGTATCCTGAAGTCCCTCCTACCAGCATCCTAGAGCATCTCCAAAGAAGGAAAATCATGAAGAGACCCAGTAGCTGCACACATACCATGTACAGTATCATGAAGTCCTGCTGGCGCTGGCGTGAGGCTGACCGCCCCTCACCTAGAGAGCTGCGCTTGCGCCTAGAAGCTGCCATTAAAACTGCAGATGACGAGGCTGTGTTACAAGTACCAGAGTTGGTGGTACCTGAACTGTATGCAGCTGTGGCCGGCATCAGAGTGGAGAGCCTCTTCTACAACTATAGCATGCTTTGAAGAGTCTCGGGCAAGAAACATTCATGCATGAGTATATGTTCTTGGAATCAATTCCTCTAAGAACAGAGAATGGTCTTTCCCAGGGACACAAAGGGAGAAATGGGACATGGATTCTTGATCTTCCTTTACACATTTCTCGGGAAATCTGAAATGATGCTGGATGGGACTCTACACATCCTGAGCTAAGACATACTGTCAGTCTCACTTCTGCTGTCCCAGTCCTAGAAATCCTGGGTAGAAGTGGTGGACCTGTGCAAAGGAGGTTTTAGAACTCTGCAGTATTTGTTGGGGCATGGCACAAATAAGCTCATCCCTCCCGTCCGAGGCTAGTTTCCTCTGGAACCACATTTTTATCTAGATGAAAATTTGGAATGAAATGAAGGAATAGAAATCCAATAAAAGAGTTGAAGGGAAAGAAAATTTAAGGTTCTTCTTGCTCAGGATTACAGATATGGACCAACACCTCCTTCAAGAAAAGGTGGTAGGACACAAAGTTCTTCAGTCCTGAGCCCTACATGTGGGGTTGGAGGAGAACTATAACGGAAAAACCTCTGAGTTTCACCTTAGGTATAGATAAAAGAAAGATGGTCCCCTTTTATCTGATTCTGAGACAGGTAAATTCTGTTTGTTACTACGTTTAATTAGAAGGTGGAGGAGTCATTTCATGATTAAGAACATTCAACATGTATTGTTCATTAAGCTAGCTTCCTAGTTCCGATTAGACTAAGGAGACTAAGCCTAGAGAGTCAATGTTAGAACAGTGAAAAGAATTCTGTGTGTGTGTGTGTGTGTGTGCACAATAAATAGGAAATGTAGAAACCAAGCAAGAAGGCTTAGTAGCTCAGTCTTTAACAAGGGCTAGAAAAGAATGTAATCTGATATGGAAGGATAGCAGCTTCTAATTTTCAATCATCTGTTGATATACTGTGAAACTTATTTTATTAAATTAATATTTATTAAATGGAAATATGCTTTTCTGGTTTATAACTACTAAAAATATCATAGGGAGGATAAAAGTAAATAAGTGAAAGTTAATGCCAATAGAAAAATTCAAGAGATAATGTACAATGTCAGAAAAGGGATTCTTTATGTGTAAATGGGGATAATACCTATTTCACAAGGTTGTTCTGAGGATTGATACGTTTTGAGTATGTATTTGTACACTATCTGGCACATATGCGCTCAATAAACGTGTTTCTCCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 32906, GC content failed 866, low tm 16781, high tm 8256, high any compl 4, high end compl 27, high repeat similarity 16, long poly-x seq 6, ok 6950
PRIMER_RIGHT_EXPLAIN=considered 32882, GC content failed 1778, low tm 16572, high tm 7682, high any compl 2, high end compl 16, high repeat similarity 13, long poly-x seq 11, ok 6808
PRIMER_PAIR_EXPLAIN=considered 691, unacceptable product size 642, high end compl 1, ok 48
PRIMER_PAIR_PENALTY=0.0173
PRIMER_LEFT_PENALTY=0.003045
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=ACTGGGCTGCAAGATATCAATAA
PRIMER_RIGHT_SEQUENCE=GCTGTGAGTAATTCCTTTGGATG
PRIMER_LEFT=86,23
PRIMER_RIGHT=214,23
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.0173
PRIMER_LEFT_1_PENALTY=0.003045
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=ACTGGGCTGCAAGATATCAATAA
PRIMER_RIGHT_1_SEQUENCE=CTGTGAGTAATTCCTTTGGATGC
PRIMER_LEFT_1=86,23
PRIMER_RIGHT_1=213,23
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=39.130
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=0.0449
PRIMER_LEFT_2_PENALTY=0.008837
PRIMER_RIGHT_2_PENALTY=0.036095
PRIMER_LEFT_2_SEQUENCE=GATGGGACTCTACACATCCTGAG
PRIMER_RIGHT_2_SEQUENCE=GAATCAGATAAAAGGGGACCATC
PRIMER_LEFT_2=1697,23
PRIMER_RIGHT_2=2143,23
PRIMER_LEFT_2_TM=60.009
PRIMER_RIGHT_2_TM=60.036
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, reverse MER39 a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=447
PRIMER_PAIR_PENALTY_3=0.0899
PRIMER_LEFT_3_PENALTY=0.088604
PRIMER_RIGHT_3_PENALTY=0.001315
PRIMER_LEFT_3_SEQUENCE=CTCACTGCTAAGCTCTGTGGATT
PRIMER_RIGHT_3_SEQUENCE=ACTGTACATGGTATGTGTGCAGC
PRIMER_LEFT_3=1055,23
PRIMER_RIGHT_3=1339,23
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.1182
PRIMER_LEFT_4_PENALTY=0.003045
PRIMER_RIGHT_4_PENALTY=0.115144
PRIMER_LEFT_4_SEQUENCE=ACTGGGCTGCAAGATATCAATAA
PRIMER_RIGHT_4_SEQUENCE=AAGTTGGGACGATAATCACTTCA
PRIMER_LEFT_4=86,23
PRIMER_RIGHT_4=362,23
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.885
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
PRIMER_PAIR_PENALTY_5=0.1249
PRIMER_LEFT_5_PENALTY=0.088773
PRIMER_RIGHT_5_PENALTY=0.036095
PRIMER_LEFT_5_SEQUENCE=AGCATCTCCAAAGAAGGAAAATC
PRIMER_RIGHT_5_SEQUENCE=GAATCAGATAAAAGGGGACCATC
PRIMER_LEFT_5=1278,23
PRIMER_RIGHT_5=2143,23
PRIMER_LEFT_5_TM=60.089
PRIMER_RIGHT_5_TM=60.036
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse MER39 a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.9000
PRIMER_RIGHT_5_END_STABILITY=8.1000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=866
PRIMER_PAIR_PENALTY_6=0.1253
PRIMER_LEFT_6_PENALTY=0.008837
PRIMER_RIGHT_6_PENALTY=0.116415
PRIMER_LEFT_6_SEQUENCE=GATGGGACTCTACACATCCTGAG
PRIMER_RIGHT_6_SEQUENCE=TGAAATGACTCCTCCACCTTCTA
PRIMER_LEFT_6=1697,23
PRIMER_RIGHT_6=2202,23
PRIMER_LEFT_6_TM=60.009
PRIMER_RIGHT_6_TM=60.116
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=6.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=506
PRIMER_PAIR_PENALTY_7=0.1294
PRIMER_LEFT_7_PENALTY=0.014220
PRIMER_RIGHT_7_PENALTY=0.115144
PRIMER_LEFT_7_SEQUENCE=GCATCCAAAGGAATTACTCACAG
PRIMER_RIGHT_7_SEQUENCE=AAGTTGGGACGATAATCACTTCA
PRIMER_LEFT_7=191,23
PRIMER_RIGHT_7=362,23
PRIMER_LEFT_7_TM=60.014
PRIMER_RIGHT_7_TM=59.885
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=172
PRIMER_PAIR_PENALTY_8=0.1294
PRIMER_LEFT_8_PENALTY=0.014220
PRIMER_RIGHT_8_PENALTY=0.115144
PRIMER_LEFT_8_SEQUENCE=CATCCAAAGGAATTACTCACAGC
PRIMER_RIGHT_8_SEQUENCE=AAGTTGGGACGATAATCACTTCA
PRIMER_LEFT_8=192,23
PRIMER_RIGHT_8=362,23
PRIMER_LEFT_8_TM=60.014
PRIMER_RIGHT_8_TM=59.885
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=171
PRIMER_PAIR_PENALTY_9=0.1357
PRIMER_LEFT_9_PENALTY=0.121486
PRIMER_RIGHT_9_PENALTY=0.014220
PRIMER_LEFT_9_SEQUENCE=CTGGGCTGCAAGATATCAATAAG
PRIMER_RIGHT_9_SEQUENCE=GCTGTGAGTAATTCCTTTGGATG
PRIMER_LEFT_9=87,23
PRIMER_RIGHT_9=214,23
PRIMER_LEFT_9_TM=60.121
PRIMER_RIGHT_9_TM=60.014
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=5.9000
PRIMER_RIGHT_9_END_STABILITY=8.1000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=128
PRIMER_PAIR_PENALTY_10=0.1357
PRIMER_LEFT_10_PENALTY=0.121486
PRIMER_RIGHT_10_PENALTY=0.014220
PRIMER_LEFT_10_SEQUENCE=CTGGGCTGCAAGATATCAATAAG
PRIMER_RIGHT_10_SEQUENCE=CTGTGAGTAATTCCTTTGGATGC
PRIMER_LEFT_10=87,23
PRIMER_RIGHT_10=213,23
PRIMER_LEFT_10_TM=60.121
PRIMER_RIGHT_10_TM=60.014
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=5.9000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=127
PRIMER_PAIR_PENALTY_11=0.1439
PRIMER_LEFT_11_PENALTY=0.142611
PRIMER_RIGHT_11_PENALTY=0.001315
PRIMER_LEFT_11_SEQUENCE=TCTTTCGAGCCAATATGAACACT
PRIMER_RIGHT_11_SEQUENCE=ACTGTACATGGTATGTGTGCAGC
PRIMER_LEFT_11=651,23
PRIMER_RIGHT_11=1339,23
PRIMER_LEFT_11_TM=60.143
PRIMER_RIGHT_11_TM=60.001
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.1000
PRIMER_RIGHT_11_END_STABILITY=9.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=689
PRIMER_PAIR_PENALTY_12=0.1457
PRIMER_LEFT_12_PENALTY=0.003045
PRIMER_RIGHT_12_PENALTY=0.142611
PRIMER_LEFT_12_SEQUENCE=ACTGGGCTGCAAGATATCAATAA
PRIMER_RIGHT_12_SEQUENCE=AGTGTTCATATTGGCTCGAAAGA
PRIMER_LEFT_12=86,23
PRIMER_RIGHT_12=673,23
PRIMER_LEFT_12_TM=60.003
PRIMER_RIGHT_12_TM=60.143
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=6.2000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=588
PRIMER_PAIR_PENALTY_13=0.1568
PRIMER_LEFT_13_PENALTY=0.014220
PRIMER_RIGHT_13_PENALTY=0.142611
PRIMER_LEFT_13_SEQUENCE=GCATCCAAAGGAATTACTCACAG
PRIMER_RIGHT_13_SEQUENCE=AGTGTTCATATTGGCTCGAAAGA
PRIMER_LEFT_13=191,23
PRIMER_RIGHT_13=673,23
PRIMER_LEFT_13_TM=60.014
PRIMER_RIGHT_13_TM=60.143
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=483
PRIMER_PAIR_PENALTY_14=0.1568
PRIMER_LEFT_14_PENALTY=0.014220
PRIMER_RIGHT_14_PENALTY=0.142611
PRIMER_LEFT_14_SEQUENCE=CATCCAAAGGAATTACTCACAGC
PRIMER_RIGHT_14_SEQUENCE=AGTGTTCATATTGGCTCGAAAGA
PRIMER_LEFT_14=192,23
PRIMER_RIGHT_14=673,23
PRIMER_LEFT_14_TM=60.014
PRIMER_RIGHT_14_TM=60.143
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=482
PRIMER_PAIR_PENALTY_15=0.1654
PRIMER_LEFT_15_PENALTY=0.050278
PRIMER_RIGHT_15_PENALTY=0.115144
PRIMER_LEFT_15_SEQUENCE=CCATATTGCATCCAAAGGAATTA
PRIMER_RIGHT_15_SEQUENCE=AAGTTGGGACGATAATCACTTCA
PRIMER_LEFT_15=184,23
PRIMER_RIGHT_15=362,23
PRIMER_LEFT_15_TM=60.050
PRIMER_RIGHT_15_TM=59.885
PRIMER_LEFT_15_GC_PERCENT=34.783
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, Tigger2 Autonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_15_END_STABILITY=6.2000
PRIMER_RIGHT_15_END_STABILITY=7.0000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=179
PRIMER_PAIR_PENALTY_16=0.1707
PRIMER_LEFT_16_PENALTY=0.134645
PRIMER_RIGHT_16_PENALTY=0.036095
PRIMER_LEFT_16_SEQUENCE=TTTCCTCTGGAACCACATTTTTA
PRIMER_RIGHT_16_SEQUENCE=GAATCAGATAAAAGGGGACCATC
PRIMER_LEFT_16=1868,23
PRIMER_RIGHT_16=2143,23
PRIMER_LEFT_16_TM=59.865
PRIMER_RIGHT_16_TM=60.036
PRIMER_LEFT_16_GC_PERCENT=34.783
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, reverse MER39 a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MER39 a consensus
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=8.1000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=276
PRIMER_PAIR_PENALTY_17=0.1929
PRIMER_LEFT_17_PENALTY=0.050278
PRIMER_RIGHT_17_PENALTY=0.142611
PRIMER_LEFT_17_SEQUENCE=CCATATTGCATCCAAAGGAATTA
PRIMER_RIGHT_17_SEQUENCE=AGTGTTCATATTGGCTCGAAAGA
PRIMER_LEFT_17=184,23
PRIMER_RIGHT_17=673,23
PRIMER_LEFT_17_TM=60.050
PRIMER_RIGHT_17_TM=60.143
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=490
PRIMER_PAIR_PENALTY_18=0.1979
PRIMER_LEFT_18_PENALTY=0.088604
PRIMER_RIGHT_18_PENALTY=0.109273
PRIMER_LEFT_18_SEQUENCE=CTCACTGCTAAGCTCTGTGGATT
PRIMER_RIGHT_18_SEQUENCE=GGTACTTGTAACACAGCCTCGTC
PRIMER_LEFT_18=1055,23
PRIMER_RIGHT_18=1455,23
PRIMER_LEFT_18_TM=60.089
PRIMER_RIGHT_18_TM=60.109
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=52.174
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, LTR12 LTR from human ERV9 endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=401
PRIMER_PAIR_PENALTY_19=0.1980
PRIMER_LEFT_19_PENALTY=0.088773
PRIMER_RIGHT_19_PENALTY=0.109273
PRIMER_LEFT_19_SEQUENCE=AGCATCTCCAAAGAAGGAAAATC
PRIMER_RIGHT_19_SEQUENCE=GGTACTTGTAACACAGCCTCGTC
PRIMER_LEFT_19=1278,23
PRIMER_RIGHT_19=1455,23
PRIMER_LEFT_19_TM=60.089
PRIMER_RIGHT_19_TM=60.109
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=1.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.9000
PRIMER_RIGHT_19_END_STABILITY=8.1000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=178
=