PRIMER_SEQUENCE_ID=NM_000314
SEQUENCE=CCTCCCCTCGCCCGGCGCGGTCCCGTCCGCCTCTCGCTCGCCTCCCGCCTCCCCTCGGTCTTCCGAGGCGCCCGGGCTCCCGGCGCGGCGGCGGAGGGGGCGGGCAGGCCGGCGGGCGGTGATGTGGCGGGACTCTTTATGCGCTGCGGCAGGATACGCGCTCGGCGCTGGGACGCGACTGCGCTCAGTTCTCTCCTCTCGGAAGCTGCAGCCATGATGGAAGTTTGAGAGTTGAGCCGCTGTGAGGCGAGGCCGGGCTCAGGCGAGGGAGATGAGAGACGGCGGCGGCCGCGGCCCGGAGCCCCTCTCAGCGCCTGTGAGCAGCCGCGGGGGCAGCGCCCTCGGGGAGCCGGCCGGCCTGCGGCGGCGGCAGCGGCGGCGTTTCTCGCCTCCTCTTCGTCTTTTCTAACCGTGCAGCCTCTTCCTCGGCTTCTCCTGAAAGGGAAGGTGGAAGCCGTGGGCTCGGGCGGGAGCCGGCTGAGGCGCGGCGGCGGCGGCGGCACCTCCCGCTCCTGGAGCGGGGGGGAGAAGCGGCGGCGGCGGCGGCCGCGGCGGCTGCAGCTCCAGGGAGGGGGTCTGAGTCGCCTGTCACCATTTCCAGGGCTGGGAACGCCGGAGAGTTGGTCTCTCCCCTTCTACTGCCTCCAACACGGCGGCGGCGGCGGCGGCACATCCAGGGACCCGGGCCGGTTTTAAACCTCCCGTCCGCCGCCGCCGCACCCCCCGTGGCCCGGGCTCCGGAGGCCGCCGGCGGAGGCAGCCGTTCGGAGGATTATTCGTCTTCTCCCCATTCCGCTGCCGCCGCTGCCAGGCCTCTGGCTGCTGAGGAGAAGCAGGCCCAGTCGCTGCAACCATCCAGCAGCCGCCGCAGCAGCCATTACCCGGCTGCGGTCCAGAGCCAAGCGGCGGCAGAGCGAGGGGCATCAGCTACCGCCAAGTCCAGAGCCATTTCCATCCTGCAGAAGAAGCCCCGCCACCAGCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCTATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTTTGTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAGTGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTTATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATAATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACAAAGCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACAAAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACCTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGATGAAGATCAGCATACACAAATTACAAAAGTCTGAATTTTTTTTTATCAAGAGGGATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATGGCAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATCTTGTTTTACCCTATACATCCACAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTGTGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGACATTTAAAATTCAATTAGGATTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGATGTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAGCTTTGTGATATACTGGTTCACATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGCAGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCTAATGCATGTATTCGGGTTAGGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATACCATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATTCGAGGAATTGGCCGCTGTCACTGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGTGCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGTACAGGAATGAACCTTCTGCAACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAGCAACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATTATAAAAATTGACAATGATTTATTAAATATGTTTTCTCAATTGTAACGACTTCTCCATCTCCTGTGTAATCAAGGCCAGTGCTAAAATTCAGATGCTGTTAGTACCTACATCAGTCAACAACTTACACTTATTTTACTAGTTTTCAATCATAATACCTGCTGTGGATGCTTCATGTGCTGCCTGCAAGCTTCTTTTTTCTCATTAAATATAAAATATTTTGTAATGCTGCACAGAAATTTTCAATTTGAGATTCTACAGTAAGCGTTTTTTTTCTTTGAAGATTTATGATGCACTTATTCAATAGCTGTCAGCCG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 38405, GC content failed 4883, low tm 17495, high tm 8685, high any compl 4, high end compl 19, high repeat similarity 52, long poly-x seq 183, ok 7084
PRIMER_RIGHT_EXPLAIN=considered 38183, GC content failed 4367, low tm 18055, high tm 8309, high any compl 1, high end compl 14, high repeat similarity 11, long poly-x seq 214, ok 7212
PRIMER_PAIR_EXPLAIN=considered 671, unacceptable product size 617, high any compl 1, high end compl 3, ok 50
PRIMER_PAIR_PENALTY=0.0205
PRIMER_LEFT_PENALTY=0.006308
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=TGTATTGGGGTACAGGAATGAAC
PRIMER_RIGHT_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT=2949,23
PRIMER_RIGHT=3082,23
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.0391
PRIMER_LEFT_1_PENALTY=0.032744
PRIMER_RIGHT_1_PENALTY=0.006308
PRIMER_LEFT_1_SEQUENCE=ATACATCCACAGGGTTTTGACAC
PRIMER_RIGHT_1_SEQUENCE=GTTCATTCCTGTACCCCAATACA
PRIMER_LEFT_1=2391,23
PRIMER_RIGHT_1=2971,23
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, LTR6 LTR from  human SSAV-related endogenous retroviral LTR-like element
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=581
PRIMER_PAIR_PENALTY_2=0.0470
PRIMER_LEFT_2_PENALTY=0.032744
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=ATACATCCACAGGGTTTTGACAC
PRIMER_RIGHT_2_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_2=2391,23
PRIMER_RIGHT_2=3082,23
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, LTR6 LTR from  human SSAV-related endogenous retroviral LTR-like element
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=692
PRIMER_PAIR_PENALTY_3=0.0547
PRIMER_LEFT_3_PENALTY=0.021925
PRIMER_RIGHT_3_PENALTY=0.032744
PRIMER_LEFT_3_SEQUENCE=AAATTTTTAAAGGCACAAGAGGC
PRIMER_RIGHT_3_SEQUENCE=GTGTCAAAACCCTGTGGATGTAT
PRIMER_LEFT_3=1460,23
PRIMER_RIGHT_3=2413,23
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=954
PRIMER_PAIR_PENALTY_4=0.0626
PRIMER_LEFT_4_PENALTY=0.014220
PRIMER_RIGHT_4_PENALTY=0.048359
PRIMER_LEFT_4_SEQUENCE=TTGACCAATGGCTAAGTGAAGAT
PRIMER_RIGHT_4_SEQUENCE=TTTGCCCCGATGTAATAAATATG
PRIMER_LEFT_4=1353,23
PRIMER_RIGHT_4=1462,23
PRIMER_LEFT_4_TM=60.014
PRIMER_RIGHT_4_TM=59.952
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=110
PRIMER_PAIR_PENALTY_5=0.0807
PRIMER_LEFT_5_PENALTY=0.066430
PRIMER_RIGHT_5_PENALTY=0.014220
PRIMER_LEFT_5_SEQUENCE=AGGCAGCTAAAGGAAGTGAATCT
PRIMER_RIGHT_5_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_5=2927,23
PRIMER_RIGHT_5=3082,23
PRIMER_LEFT_5_TM=59.934
PRIMER_RIGHT_5_TM=60.014
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=6.6000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=156
PRIMER_PAIR_PENALTY_6=0.0811
PRIMER_LEFT_6_PENALTY=0.048359
PRIMER_RIGHT_6_PENALTY=0.032744
PRIMER_LEFT_6_SEQUENCE=CATATTTATTACATCGGGGCAAA
PRIMER_RIGHT_6_SEQUENCE=GTGTCAAAACCCTGTGGATGTAT
PRIMER_LEFT_6=1440,23
PRIMER_RIGHT_6=2413,23
PRIMER_LEFT_6_TM=59.952
PRIMER_RIGHT_6_TM=60.033
PRIMER_LEFT_6_GC_PERCENT=34.783
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_6_END_STABILITY=8.8000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=974
PRIMER_PAIR_PENALTY_7=0.0934
PRIMER_LEFT_7_PENALTY=0.014220
PRIMER_RIGHT_7_PENALTY=0.079168
PRIMER_LEFT_7_SEQUENCE=TTGACCAATGGCTAAGTGAAGAT
PRIMER_RIGHT_7_SEQUENCE=GTACATGAACTTGTCTTCCCGTC
PRIMER_LEFT_7=1353,23
PRIMER_RIGHT_7=1747,23
PRIMER_LEFT_7_TM=60.014
PRIMER_RIGHT_7_TM=59.921
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=6.6000
PRIMER_RIGHT_7_END_STABILITY=9.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=395
PRIMER_PAIR_PENALTY_8=0.0949
PRIMER_LEFT_8_PENALTY=0.088543
PRIMER_RIGHT_8_PENALTY=0.006308
PRIMER_LEFT_8_SEQUENCE=TGGACTCTGGACCATATACCATC
PRIMER_RIGHT_8_SEQUENCE=GTTCATTCCTGTACCCCAATACA
PRIMER_LEFT_8=2780,23
PRIMER_RIGHT_8=2971,23
PRIMER_LEFT_8_TM=60.089
PRIMER_RIGHT_8_TM=59.994
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.1000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=192
PRIMER_PAIR_PENALTY_9=0.1011
PRIMER_LEFT_9_PENALTY=0.021925
PRIMER_RIGHT_9_PENALTY=0.079168
PRIMER_LEFT_9_SEQUENCE=AAATTTTTAAAGGCACAAGAGGC
PRIMER_RIGHT_9_SEQUENCE=GTACATGAACTTGTCTTCCCGTC
PRIMER_LEFT_9=1460,23
PRIMER_RIGHT_9=1747,23
PRIMER_LEFT_9_TM=60.022
PRIMER_RIGHT_9_TM=59.921
PRIMER_LEFT_9_GC_PERCENT=34.783
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=288
PRIMER_PAIR_PENALTY_10=0.1028
PRIMER_LEFT_10_PENALTY=0.088543
PRIMER_RIGHT_10_PENALTY=0.014220
PRIMER_LEFT_10_SEQUENCE=TGGACTCTGGACCATATACCATC
PRIMER_RIGHT_10_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_10=2780,23
PRIMER_RIGHT_10=3082,23
PRIMER_LEFT_10_TM=60.089
PRIMER_RIGHT_10_TM=60.014
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=6.6000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=303
PRIMER_PAIR_PENALTY_11=0.1170
PRIMER_LEFT_11_PENALTY=0.068602
PRIMER_RIGHT_11_PENALTY=0.048359
PRIMER_LEFT_11_SEQUENCE=ACTTGAAGGCGTATACAGGAACA
PRIMER_RIGHT_11_SEQUENCE=TTTGCCCCGATGTAATAAATATG
PRIMER_LEFT_11=1153,23
PRIMER_RIGHT_11=1462,23
PRIMER_LEFT_11_TM=60.069
PRIMER_RIGHT_11_TM=59.952
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=34.783
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=5.8000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=310
PRIMER_PAIR_PENALTY_12=0.1219
PRIMER_LEFT_12_PENALTY=0.115612
PRIMER_RIGHT_12_PENALTY=0.006308
PRIMER_LEFT_12_SEQUENCE=CCTATACATCCACAGGGTTTTGA
PRIMER_RIGHT_12_SEQUENCE=GTTCATTCCTGTACCCCAATACA
PRIMER_LEFT_12=2388,23
PRIMER_RIGHT_12=2971,23
PRIMER_LEFT_12_TM=60.116
PRIMER_RIGHT_12_TM=59.994
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=5.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=584
PRIMER_PAIR_PENALTY_13=0.1275
PRIMER_LEFT_13_PENALTY=0.048359
PRIMER_RIGHT_13_PENALTY=0.079168
PRIMER_LEFT_13_SEQUENCE=CATATTTATTACATCGGGGCAAA
PRIMER_RIGHT_13_SEQUENCE=GTACATGAACTTGTCTTCCCGTC
PRIMER_LEFT_13=1440,23
PRIMER_RIGHT_13=1747,23
PRIMER_LEFT_13_TM=59.952
PRIMER_RIGHT_13_TM=59.921
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_13_END_STABILITY=8.8000
PRIMER_RIGHT_13_END_STABILITY=9.6000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=308
PRIMER_PAIR_PENALTY_14=0.1298
PRIMER_LEFT_14_PENALTY=0.115612
PRIMER_RIGHT_14_PENALTY=0.014220
PRIMER_LEFT_14_SEQUENCE=CCTATACATCCACAGGGTTTTGA
PRIMER_RIGHT_14_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_14=2388,23
PRIMER_RIGHT_14=3082,23
PRIMER_LEFT_14_TM=60.116
PRIMER_RIGHT_14_TM=60.014
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=695
PRIMER_PAIR_PENALTY_15=0.1298
PRIMER_LEFT_15_PENALTY=0.115612
PRIMER_RIGHT_15_PENALTY=0.014220
PRIMER_LEFT_15_SEQUENCE=CTGTATTGGGGTACAGGAATGAA
PRIMER_RIGHT_15_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_15=2948,23
PRIMER_RIGHT_15=3082,23
PRIMER_LEFT_15_TM=60.116
PRIMER_RIGHT_15_TM=60.014
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.9000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_15=135
PRIMER_PAIR_PENALTY_16=0.1363
PRIMER_LEFT_16_PENALTY=0.122060
PRIMER_RIGHT_16_PENALTY=0.014220
PRIMER_LEFT_16_SEQUENCE=AGGAAGTGAATCTGTATTGGGGT
PRIMER_RIGHT_16_SEQUENCE=TCTTTGTTGATAGCCTCCACATT
PRIMER_LEFT_16=2937,23
PRIMER_RIGHT_16=3082,23
PRIMER_LEFT_16_TM=60.122
PRIMER_RIGHT_16_TM=60.014
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=1.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, reverse L1MB7 3'-end of L1 repeat (subfamily L1MB7) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=10.6000
PRIMER_RIGHT_16_END_STABILITY=6.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=146
PRIMER_PAIR_PENALTY_17=0.1363
PRIMER_LEFT_17_PENALTY=0.014220
PRIMER_RIGHT_17_PENALTY=0.122120
PRIMER_LEFT_17_SEQUENCE=TTGACCAATGGCTAAGTGAAGAT
PRIMER_RIGHT_17_SEQUENCE=TCTGGTCCTGGTATGAAGAATGT
PRIMER_LEFT_17=1353,23
PRIMER_RIGHT_17=1878,23
PRIMER_LEFT_17_TM=60.014
PRIMER_RIGHT_17_TM=59.878
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER44A Nonautonomous DNA transposon
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.6000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=526
PRIMER_PAIR_PENALTY_18=0.1368
PRIMER_LEFT_18_PENALTY=0.088469
PRIMER_RIGHT_18_PENALTY=0.048359
PRIMER_LEFT_18_SEQUENCE=TTGAAGGCGTATACAGGAACAAT
PRIMER_RIGHT_18_SEQUENCE=TTTGCCCCGATGTAATAAATATG
PRIMER_LEFT_18=1155,23
PRIMER_RIGHT_18=1462,23
PRIMER_LEFT_18_TM=59.912
PRIMER_RIGHT_18_TM=59.952
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.6000
PRIMER_RIGHT_18_END_STABILITY=5.8000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=308
PRIMER_PAIR_PENALTY_19=0.1440
PRIMER_LEFT_19_PENALTY=0.021925
PRIMER_RIGHT_19_PENALTY=0.122120
PRIMER_LEFT_19_SEQUENCE=AAATTTTTAAAGGCACAAGAGGC
PRIMER_RIGHT_19_SEQUENCE=TCTGGTCCTGGTATGAAGAATGT
PRIMER_LEFT_19=1460,23
PRIMER_RIGHT_19=1878,23
PRIMER_LEFT_19_TM=60.022
PRIMER_RIGHT_19_TM=59.878
PRIMER_LEFT_19_GC_PERCENT=34.783
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=9.4000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=419
=