PRIMER_SEQUENCE_ID=NM_000345
SEQUENCE=GGAGTGGCCATTCGACGACAGTGTGGTGTAAAGGAATTCATTAGCCATGGATGTATTCATGAAAGGACTTTCAAAGGCCAAGGAGGGAGTTGTGGCTGCTGCTGAGAAAACCAAACAGGGTGTGGCAGAAGCAGCAGGAAAGACAAAAGAGGGTGTTCTCTATGTAGGCTCCAAAACCAAGGAGGGAGTGGTGCATGGTGTGGCAACAGTGGCTGAGAAGACCAAAGAGCAAGTGACAAATGTTGGAGGAGCAGTGGTGACGGGTGTGACAGCAGTAGCCCAGAAGACAGTGGAGGGAGCAGGGAGCATTGCAGCAGCCACTGGCTTTGTCAAAAAGGACCAGTTGGGCAAGAATGAAGAAGGAGCCCCACAGGAAGGAATTCTGGAAGATATGCCTGTGGATCCTGACAATGAGGCTTATGAAATGCCTTCTGAGGAAGGGTATCAAGACTACGAACCTGAAGCCTAAGAAATATCTTTGCTCCCAGTTTCTTGAGATCTGCTGACAGATGTTCCATCCTGTACAAGTGCTCAGTTCCAATGTGCCCAGTCATGACATTTCTCAAAGTTTTTACAGTGTATCTCGAAGTCTTCCATCAGCAGTGATTGAAGTATCTGTACCTGCCCCCACTCAGCATTTCGGTGCTTCCCTTTCACTGAAGTGAATACATGGTAGCAGGGTCTTTGTGTGCTGTGGATTTTGTGGCTTCAATCTACGATGTTAAAACAAATTAAAAACACCTAAGTGACTACCACTTATTTCTAAATCCTCACTATTTTTTTGTTGCTGTTGTTCAGAAGTTGTTAGTGATTTGCTATCATATATTATAAGATTTTTAGGTGTCTTTTAATGATACTGTCTAAGAATAATGACGTATTGTGAAATTTGTTAATATATATAATACTTAAAAATATGTGAGCATGAAACTATGCACCTATAAATACTAAATATGAAATTTTACCATTTTGCGATGTGTTTTATTCACTTGTGTTTGTATATAAATGGTGAGAATTAAAATAAAACGTTATCTCATTGCAAAAATATTTTATTTTTATCCCATCTCACTTTAATAATAAAAATCATGCTTATAAGCAACATGAATTAAGAACTGACACAAAGGACAAAAATATAAAGTTATTAATAGCCATTTGAAGAAGGAGGAATTTTAGAAGAGGTAGAGAAAATGGAACATTAACCCTACACTCGGAATTCCCTGAAGCAACACTGCCAGAAGTGTGTTTTGGTATGCACTGGTTCCTTAAGTGGCTGTGATTAATTATTGAAAGTGGGGTGTTGAAGACCCCAACTACTATTGTAGAGTGGTCTATTTCTCCCTTCAATCCTGTCAATGTTTGCTTTATGTATTTTGGGGAACTGTTGTTTGATGTGTATGTGTTTATAATTGTTATACATTTTTAATTGAGCCTTTTATTAACATATATTGTTATTTTTGTCTCGAAATAATTTTTTAGTTAAAATCTATTTTGTCTGATATTGGTGTGAATGCTGTACCTTTCTGACAATAAATAATATTCGACCATG
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 17152, GC content failed 1717, low tm 9184, high tm 3000, high any compl 2, high end compl 24, high repeat similarity 28, long poly-x seq 22, ok 3175
PRIMER_RIGHT_EXPLAIN=considered 17110, GC content failed 1988, low tm 9230, high tm 2792, high end compl 4, high repeat similarity 13, long poly-x seq 29, ok 3054
PRIMER_PAIR_EXPLAIN=considered 191, unacceptable product size 139, high end compl 9, ok 43
PRIMER_PAIR_PENALTY=0.0684
PRIMER_LEFT_PENALTY=0.020535
PRIMER_RIGHT_PENALTY=0.047847
PRIMER_LEFT_SEQUENCE=GAATTCTGGAAGATATGCCTGTG
PRIMER_RIGHT_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT=377,23
PRIMER_RIGHT=988,23
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=34.783
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=612
PRIMER_PAIR_PENALTY_1=0.0768
PRIMER_LEFT_1_PENALTY=0.028939
PRIMER_RIGHT_1_PENALTY=0.047847
PRIMER_LEFT_1_SEQUENCE=AGGAAAGACAAAAGAGGGTGTTC
PRIMER_RIGHT_1_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT_1=135,23
PRIMER_RIGHT_1=988,23
PRIMER_LEFT_1_TM=60.029
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=34.783
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=854
PRIMER_PAIR_PENALTY_2=0.0768
PRIMER_LEFT_2_PENALTY=0.028939
PRIMER_RIGHT_2_PENALTY=0.047847
PRIMER_LEFT_2_SEQUENCE=GGAAAGACAAAAGAGGGTGTTCT
PRIMER_RIGHT_2_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT_2=136,23
PRIMER_RIGHT_2=988,23
PRIMER_LEFT_2_TM=60.029
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=853
PRIMER_PAIR_PENALTY_3=0.0784
PRIMER_LEFT_3_PENALTY=0.020535
PRIMER_RIGHT_3_PENALTY=0.057845
PRIMER_LEFT_3_SEQUENCE=GAATTCTGGAAGATATGCCTGTG
PRIMER_RIGHT_3_SEQUENCE=AGCACTTGTACAGGATGGAACAT
PRIMER_LEFT_3=377,23
PRIMER_RIGHT_3=531,23
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.942
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.0868
PRIMER_LEFT_4_PENALTY=0.028939
PRIMER_RIGHT_4_PENALTY=0.057845
PRIMER_LEFT_4_SEQUENCE=GGAAAGACAAAAGAGGGTGTTCT
PRIMER_RIGHT_4_SEQUENCE=AGCACTTGTACAGGATGGAACAT
PRIMER_LEFT_4=136,23
PRIMER_RIGHT_4=531,23
PRIMER_LEFT_4_TM=60.029
PRIMER_RIGHT_4_TM=59.942
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=396
PRIMER_PAIR_PENALTY_5=0.1057
PRIMER_LEFT_5_PENALTY=0.057845
PRIMER_RIGHT_5_PENALTY=0.047847
PRIMER_LEFT_5_SEQUENCE=ATGTTCCATCCTGTACAAGTGCT
PRIMER_RIGHT_5_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT_5=509,23
PRIMER_RIGHT_5=988,23
PRIMER_LEFT_5_TM=59.942
PRIMER_RIGHT_5_TM=60.048
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=34.783
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=10.5000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=480
PRIMER_PAIR_PENALTY_6=0.1133
PRIMER_LEFT_6_PENALTY=0.055462
PRIMER_RIGHT_6_PENALTY=0.057845
PRIMER_LEFT_6_SEQUENCE=GGAATTCATTAGCCATGGATGTA
PRIMER_RIGHT_6_SEQUENCE=AGCACTTGTACAGGATGGAACAT
PRIMER_LEFT_6=32,23
PRIMER_RIGHT_6=531,23
PRIMER_LEFT_6_TM=60.055
PRIMER_RIGHT_6_TM=59.942
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=3.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.6000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=500
PRIMER_PAIR_PENALTY_7=0.1171
PRIMER_LEFT_7_PENALTY=0.057845
PRIMER_RIGHT_7_PENALTY=0.059210
PRIMER_LEFT_7_SEQUENCE=ATGTTCCATCCTGTACAAGTGCT
PRIMER_RIGHT_7_SEQUENCE=ACTTAAGGAACCAGTGCATACCA
PRIMER_LEFT_7=509,23
PRIMER_RIGHT_7=1264,23
PRIMER_LEFT_7_TM=59.942
PRIMER_RIGHT_7_TM=59.941
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=756
PRIMER_PAIR_PENALTY_8=0.1171
PRIMER_LEFT_8_PENALTY=0.057845
PRIMER_RIGHT_8_PENALTY=0.059210
PRIMER_LEFT_8_SEQUENCE=ATGTTCCATCCTGTACAAGTGCT
PRIMER_RIGHT_8_SEQUENCE=CACTTAAGGAACCAGTGCATACC
PRIMER_LEFT_8=509,23
PRIMER_RIGHT_8=1265,23
PRIMER_LEFT_8_TM=59.942
PRIMER_RIGHT_8_TM=59.941
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=6.8000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=757
PRIMER_PAIR_PENALTY_9=0.1171
PRIMER_LEFT_9_PENALTY=0.057845
PRIMER_RIGHT_9_PENALTY=0.059210
PRIMER_LEFT_9_SEQUENCE=ATGTTCCATCCTGTACAAGTGCT
PRIMER_RIGHT_9_SEQUENCE=CCACTTAAGGAACCAGTGCATAC
PRIMER_LEFT_9=509,23
PRIMER_RIGHT_9=1266,23
PRIMER_LEFT_9_TM=59.942
PRIMER_RIGHT_9_TM=59.941
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=5.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=758
PRIMER_PAIR_PENALTY_10=0.1175
PRIMER_LEFT_10_PENALTY=0.028939
PRIMER_RIGHT_10_PENALTY=0.088604
PRIMER_LEFT_10_SEQUENCE=GGAAAGACAAAAGAGGGTGTTCT
PRIMER_RIGHT_10_SEQUENCE=ACATTTGTCACTTGCTCTTTGGT
PRIMER_LEFT_10=136,23
PRIMER_RIGHT_10=242,23
PRIMER_LEFT_10_TM=60.029
PRIMER_RIGHT_10_TM=60.089
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=7.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=107
PRIMER_PAIR_PENALTY_11=0.1441
PRIMER_LEFT_11_PENALTY=0.055462
PRIMER_RIGHT_11_PENALTY=0.088604
PRIMER_LEFT_11_SEQUENCE=GGAATTCATTAGCCATGGATGTA
PRIMER_RIGHT_11_SEQUENCE=ACATTTGTCACTTGCTCTTTGGT
PRIMER_LEFT_11=32,23
PRIMER_RIGHT_11=242,23
PRIMER_LEFT_11_TM=60.055
PRIMER_RIGHT_11_TM=60.089
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR7 LTR from human endogenous retrovirus RTVL-H2
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.6000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=211
PRIMER_PAIR_PENALTY_12=0.1836
PRIMER_LEFT_12_PENALTY=0.135712
PRIMER_RIGHT_12_PENALTY=0.047847
PRIMER_LEFT_12_SEQUENCE=TCTCTATGTAGGCTCCAAAACCA
PRIMER_RIGHT_12_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT_12=156,23
PRIMER_RIGHT_12=988,23
PRIMER_LEFT_12_TM=60.136
PRIMER_RIGHT_12_TM=60.048
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.2000
PRIMER_RIGHT_12_END_STABILITY=10.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=833
PRIMER_PAIR_PENALTY_13=0.1927
PRIMER_LEFT_13_PENALTY=0.028939
PRIMER_RIGHT_13_PENALTY=0.163755
PRIMER_LEFT_13_SEQUENCE=AGGAAAGACAAAAGAGGGTGTTC
PRIMER_RIGHT_13_SEQUENCE=CAACATTTGTCACTTGCTCTTTG
PRIMER_LEFT_13=135,23
PRIMER_RIGHT_13=244,23
PRIMER_LEFT_13_TM=60.029
PRIMER_RIGHT_13_TM=59.836
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=7.3000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=110
PRIMER_PAIR_PENALTY_14=0.1927
PRIMER_LEFT_14_PENALTY=0.028939
PRIMER_RIGHT_14_PENALTY=0.163755
PRIMER_LEFT_14_SEQUENCE=GGAAAGACAAAAGAGGGTGTTCT
PRIMER_RIGHT_14_SEQUENCE=CAACATTTGTCACTTGCTCTTTG
PRIMER_LEFT_14=136,23
PRIMER_RIGHT_14=244,23
PRIMER_LEFT_14_TM=60.029
PRIMER_RIGHT_14_TM=59.836
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=3.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.3000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=109
PRIMER_PAIR_PENALTY_15=0.1936
PRIMER_LEFT_15_PENALTY=0.135712
PRIMER_RIGHT_15_PENALTY=0.057845
PRIMER_LEFT_15_SEQUENCE=TCTCTATGTAGGCTCCAAAACCA
PRIMER_RIGHT_15_SEQUENCE=AGCACTTGTACAGGATGGAACAT
PRIMER_LEFT_15=156,23
PRIMER_RIGHT_15=531,23
PRIMER_LEFT_15_TM=60.136
PRIMER_RIGHT_15_TM=59.942
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=3.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.6000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=376
PRIMER_PAIR_PENALTY_16=0.2014
PRIMER_LEFT_16_PENALTY=0.153589
PRIMER_RIGHT_16_PENALTY=0.047847
PRIMER_LEFT_16_SEQUENCE=AGATCTGCTGACAGATGTTCCAT
PRIMER_RIGHT_16_SEQUENCE=CAAGTGAATAAAACACATCGCAA
PRIMER_LEFT_16=495,23
PRIMER_RIGHT_16=988,23
PRIMER_LEFT_16_TM=60.154
PRIMER_RIGHT_16_TM=60.048
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=34.783
PRIMER_LEFT_16_SELF_ANY=7.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=8.1000
PRIMER_RIGHT_16_END_STABILITY=10.5000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=494
PRIMER_PAIR_PENALTY_17=0.2128
PRIMER_LEFT_17_PENALTY=0.153589
PRIMER_RIGHT_17_PENALTY=0.059210
PRIMER_LEFT_17_SEQUENCE=AGATCTGCTGACAGATGTTCCAT
PRIMER_RIGHT_17_SEQUENCE=ACTTAAGGAACCAGTGCATACCA
PRIMER_LEFT_17=495,23
PRIMER_RIGHT_17=1264,23
PRIMER_LEFT_17_TM=60.154
PRIMER_RIGHT_17_TM=59.941
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=7.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=8.1000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=770
PRIMER_PAIR_PENALTY_18=0.2128
PRIMER_LEFT_18_PENALTY=0.153589
PRIMER_RIGHT_18_PENALTY=0.059210
PRIMER_LEFT_18_SEQUENCE=AGATCTGCTGACAGATGTTCCAT
PRIMER_RIGHT_18_SEQUENCE=CACTTAAGGAACCAGTGCATACC
PRIMER_LEFT_18=495,23
PRIMER_RIGHT_18=1265,23
PRIMER_LEFT_18_TM=60.154
PRIMER_RIGHT_18_TM=59.941
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, LTR13 LTR from a  putative human endogenous retrovirus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.1000
PRIMER_RIGHT_18_END_STABILITY=6.8000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=771
PRIMER_PAIR_PENALTY_19=0.2128
PRIMER_LEFT_19_PENALTY=0.153589
PRIMER_RIGHT_19_PENALTY=0.059210
PRIMER_LEFT_19_SEQUENCE=AGATCTGCTGACAGATGTTCCAT
PRIMER_RIGHT_19_SEQUENCE=CCACTTAAGGAACCAGTGCATAC
PRIMER_LEFT_19=495,23
PRIMER_RIGHT_19=1266,23
PRIMER_LEFT_19_TM=60.154
PRIMER_RIGHT_19_TM=59.941
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=5.6000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=772
=