PRIMER_SEQUENCE_ID=NM_001395
SEQUENCE=CGCTTCCCGCCGCCCGAGCTTCGGAAACTTCCCGGCCGCGACGCAGGGAACCGGCGCGGAGAACCGAGCAGAGCGGAGCGCCCGTGGTCCAGCGTGTAGGGAGCCGATCGCCCATGGAGGGTCTGGGCCGCTCGTGCCTGTGGCTGCGTCGGGAGCTGTCGCCCCCGCGGCCGCGGCTCCTGCTCCTGGACTGCCGCAGCCGCGAGCTGTACGAGTCGGCGCGCATCGGTGGGGCGCTGAGCGTGGCCCTGCCGGCGCTCCTGCTGCGCCGCCTGCGGAGGGGCAGCCTGTCGGTGCGCGCGCTCCTGCCTGGGCCGCCGCTGCAGCCGCCCCCGCCTGCCCCCGTGCTCCTGTACGACCAGGGCGGGGGCCGGCGCCGGCGCGGGGAGGCCGAGGCCGAGGCCGAGGAGTGGGAGGCCGAGTCGGTGCTGGGCACCCTGCTGCAGAAGCTGCGAGAGGAAGGCTACCTGGCCTACTACCTCCAGGGAGGCTTCAGCAGATTCCAGGCCGAGTGCCCTCACCTGTGTGAGACCAGCCTTGCTGGCCGTGCCGGCTCCAGCATGGCGCCGGTGCCCGGTCCAGTGCCCGTGGTGGGGTTGGGCAGCCTGTGCCTGGGCTCCGACTGCTCTGATGCGGAATCCGAGGCTGACCGCGACTCCATGAGCTGTGGCCTGGATTCGGAGGGTGCCACACCCCCACCAGTGGGGCTGCGGGCATCCTTCCCTGTCCAGATCCTGCCCAACCTCTATCTGGGCAGTGCCCGGGATTCCGCCAATTTGGAGAGCCTGGCCAAACTGGGCATCCGCTACATCCTCAATGTCACCCCCAACCTCCCAAACTTCTTCGAGAAGAATGGTGACTTTCACTACAAGCAGATCCCCATCTCCGACCACTGGAGCCAGAACCTGTCGCGGTTCTTTCCGGAGGCCATTGAGTTCATTGATGAGGCCTTGTCCCAGAACTGCGGGGTGCTCGTCCACTGCTTGGCGGGGGTCAGCCGTTCTGTCACCGTCACTGTGGCCTACCTCATGCAGAAGCTCCACCTCTCTCTCAACGATGCCTATGACCTGGTCAAGAGGAAGAAGTCTAACATCTCCCCCAACTTCAACTTCATGGGGCAGTTGCTGGACTTTGAGCGCAGCTTGCGGCTGGAGGAGCGCCACTCGCAGGAGCAGGGCAGTGGGGGGCAGGCATCTGCGGCCTCCAACCCGCCCTCCTTCTTCACCACCCCCACCAGTGATGGCGCCTTCGAGCTGGCCCCCACCTAGGGCCCCGTGGCCGGCAGGCCGGCCCCTGCCCCACCCCCACCCACGGGTGTCCCTGCCCACTCGTGTGGCAAGGGAGGGGAGGGCAGGAGGGCTCGGCCTGAGCAGGGTGCTGGGGGGAGAGCGCAATACCTCACGCGGGCTGCCGTCCTAATCAACGTGCCTATGGCGGGACCACGCTCGGAGCCTGCCTCTTCTGCGACTGTTACTTTTTCTTTGCGGGATGGGGGTGGGGGTTCCCTCTCCAGGTGGTTGTCCAAGCCCAGGTCCCGGCCCTGGGTGCTCAGCCAGCTCGGCTAGGCCCTGCGCCTCCCTGCGCTTCCCCCTTCAGGAAGGGTGTGTGCCACCTCGTTGCACTGGATCCCAGTGGCTGCTTGGGGGAGAGGCGTTTGCCATCACTGGTGTTGTCACCTCCCTGTTTCTCCACCAAGGGCTTGGGCCTCTCGGGGCTGGGGCCTCCCAGGGGATGGGGACCCAGAGGTGCAGTGGCCGCCCACATCCATGGCCTAGGAGCTACTGGGCAGGTTCCCGGCCACACATCTGGTGGGCTGTTTTGTTTTTTTTTTTCCTCTTCCCCCAGATGTCTTGACGGGATCACTGGGGCTCTTTGTGAGTGAGGGTGGCCAAACTACCGCCGGAGGAGATGGGGTCTCAGAGCGAGAGCTGCGGAGGGGGAGGGGAAGAAGAAGGCCTCACTTTTGCTGCTGCGGGGCCCACACAGCCGCTGCTACTTTGGGGGGTGGGGAAGGGGCCAAGCTGCAGACACACACAGTCATTCATTTCTGTCCACACCCCTGTGGGTGGCGGGTGTGCGTGTGTGTGCTTGTGTGTGCGCACGTGTCGGCGCTCACACACACATGCTAGCCCACTGATGCACCCAGCCCAGGGCTGGCAGTCTTTGCAGCGTGGGGCCGTCTCACCCTGGAGCCTGGAGAGGATCTATGCTTGTTTGTTTTTGTAATCCATATCATAGTTGCTTTCTTTAATTGTTCCTTCTGAATAAACAGTTTATTTAAGATAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 25757, GC content failed 2506, low tm 3652, high tm 15683, high any compl 18, high end compl 17, high repeat similarity 16, long poly-x seq 89, ok 3776
PRIMER_RIGHT_EXPLAIN=considered 25757, GC content failed 2665, low tm 4243, high tm 14953, high any compl 2, high end compl 12, high repeat similarity 7, long poly-x seq 89, ok 3786
PRIMER_PAIR_EXPLAIN=considered 475, unacceptable product size 423, high end compl 2, ok 50
PRIMER_PAIR_PENALTY=0.1468
PRIMER_LEFT_PENALTY=0.034339
PRIMER_RIGHT_PENALTY=0.112479
PRIMER_LEFT_SEQUENCE=TGGTGACTTTCACTACAAGCAGA
PRIMER_RIGHT_SEQUENCE=GAAGTTGGGGGAGATGTTAGACT
PRIMER_LEFT=853,23
PRIMER_RIGHT=1105,23
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.888
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.3098
PRIMER_LEFT_1_PENALTY=0.197354
PRIMER_RIGHT_1_PENALTY=0.112479
PRIMER_LEFT_1_SEQUENCE=CGAGAAGAATGGTGACTTTCACT
PRIMER_RIGHT_1_SEQUENCE=GAAGTTGGGGGAGATGTTAGACT
PRIMER_LEFT_1=844,23
PRIMER_RIGHT_1=1105,23
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.888
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.6018
PRIMER_LEFT_2_PENALTY=0.112479
PRIMER_RIGHT_2_PENALTY=0.489294
PRIMER_LEFT_2_SEQUENCE=AGTCTAACATCTCCCCCAACTTC
PRIMER_RIGHT_2_SEQUENCE=GGACAGAAATGAATGACTGTGTG
PRIMER_LEFT_2=1083,23
PRIMER_RIGHT_2=2052,23
PRIMER_LEFT_2_TM=59.888
PRIMER_RIGHT_2_TM=59.511
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=970
PRIMER_PAIR_PENALTY_3=0.6018
PRIMER_LEFT_3_PENALTY=0.112479
PRIMER_RIGHT_3_PENALTY=0.489294
PRIMER_LEFT_3_SEQUENCE=AGTCTAACATCTCCCCCAACTTC
PRIMER_RIGHT_3_SEQUENCE=TGGACAGAAATGAATGACTGTGT
PRIMER_LEFT_3=1083,23
PRIMER_RIGHT_3=2053,23
PRIMER_LEFT_3_TM=59.888
PRIMER_RIGHT_3_TM=59.511
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=971
PRIMER_PAIR_PENALTY_4=0.6018
PRIMER_LEFT_4_PENALTY=0.112479
PRIMER_RIGHT_4_PENALTY=0.489294
PRIMER_LEFT_4_SEQUENCE=GAAGTCTAACATCTCCCCCAACT
PRIMER_RIGHT_4_SEQUENCE=GGACAGAAATGAATGACTGTGTG
PRIMER_LEFT_4=1081,23
PRIMER_RIGHT_4=2052,23
PRIMER_LEFT_4_TM=59.888
PRIMER_RIGHT_4_TM=59.511
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=972
PRIMER_PAIR_PENALTY_5=0.6018
PRIMER_LEFT_5_PENALTY=0.112479
PRIMER_RIGHT_5_PENALTY=0.489294
PRIMER_LEFT_5_SEQUENCE=GAAGTCTAACATCTCCCCCAACT
PRIMER_RIGHT_5_SEQUENCE=TGGACAGAAATGAATGACTGTGT
PRIMER_LEFT_5=1081,23
PRIMER_RIGHT_5=2053,23
PRIMER_LEFT_5_TM=59.888
PRIMER_RIGHT_5_TM=59.511
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_5_END_STABILITY=6.7000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=973
PRIMER_PAIR_PENALTY_6=0.6018
PRIMER_LEFT_6_PENALTY=0.112479
PRIMER_RIGHT_6_PENALTY=0.489294
PRIMER_LEFT_6_SEQUENCE=AGAAGTCTAACATCTCCCCCAAC
PRIMER_RIGHT_6_SEQUENCE=GGACAGAAATGAATGACTGTGTG
PRIMER_LEFT_6=1080,23
PRIMER_RIGHT_6=2052,23
PRIMER_LEFT_6_TM=59.888
PRIMER_RIGHT_6_TM=59.511
PRIMER_LEFT_6_GC_PERCENT=47.826
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=6.4000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=973
PRIMER_PAIR_PENALTY_7=0.6018
PRIMER_LEFT_7_PENALTY=0.112479
PRIMER_RIGHT_7_PENALTY=0.489294
PRIMER_LEFT_7_SEQUENCE=AGAAGTCTAACATCTCCCCCAAC
PRIMER_RIGHT_7_SEQUENCE=TGGACAGAAATGAATGACTGTGT
PRIMER_LEFT_7=1080,23
PRIMER_RIGHT_7=2053,23
PRIMER_LEFT_7_TM=59.888
PRIMER_RIGHT_7_TM=59.511
PRIMER_LEFT_7_GC_PERCENT=47.826
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=974
PRIMER_PAIR_PENALTY_8=0.6018
PRIMER_LEFT_8_PENALTY=0.034339
PRIMER_RIGHT_8_PENALTY=0.567488
PRIMER_LEFT_8_SEQUENCE=TGGTGACTTTCACTACAAGCAGA
PRIMER_RIGHT_8_SEQUENCE=AAGTTGGGGGAGATGTTAGACTT
PRIMER_LEFT_8=853,23
PRIMER_RIGHT_8=1104,23
PRIMER_LEFT_8_TM=59.966
PRIMER_RIGHT_8_TM=59.433
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=252
PRIMER_PAIR_PENALTY_9=0.6728
PRIMER_LEFT_9_PENALTY=0.034339
PRIMER_RIGHT_9_PENALTY=0.638440
PRIMER_LEFT_9_SEQUENCE=TGGTGACTTTCACTACAAGCAGA
PRIMER_RIGHT_9_SEQUENCE=ATAGGCATCGTTGAGAGAGAGGT
PRIMER_LEFT_9=853,23
PRIMER_RIGHT_9=1063,23
PRIMER_LEFT_9_TM=59.966
PRIMER_RIGHT_9_TM=60.638
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=211
PRIMER_PAIR_PENALTY_10=0.7104
PRIMER_LEFT_10_PENALTY=0.597928
PRIMER_RIGHT_10_PENALTY=0.112479
PRIMER_LEFT_10_SEQUENCE=CTTCGAGAAGAATGGTGACTTTC
PRIMER_RIGHT_10_SEQUENCE=GAAGTTGGGGGAGATGTTAGACT
PRIMER_LEFT_10=841,23
PRIMER_RIGHT_10=1105,23
PRIMER_LEFT_10_TM=59.402
PRIMER_RIGHT_10_TM=59.888
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=6.1000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=265
PRIMER_PAIR_PENALTY_11=0.7104
PRIMER_LEFT_11_PENALTY=0.597928
PRIMER_RIGHT_11_PENALTY=0.112479
PRIMER_LEFT_11_SEQUENCE=TCTTCGAGAAGAATGGTGACTTT
PRIMER_RIGHT_11_SEQUENCE=GAAGTTGGGGGAGATGTTAGACT
PRIMER_LEFT_11=840,23
PRIMER_RIGHT_11=1105,23
PRIMER_LEFT_11_TM=59.402
PRIMER_RIGHT_11_TM=59.888
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=8.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.7000
PRIMER_RIGHT_11_END_STABILITY=6.1000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=266
PRIMER_PAIR_PENALTY_12=0.7133
PRIMER_LEFT_12_PENALTY=0.095861
PRIMER_RIGHT_12_PENALTY=0.617487
PRIMER_LEFT_12_SEQUENCE=GCAGACACACACAGTCATTCATT
PRIMER_RIGHT_12_SEQUENCE=CAAACAAGCATAGATCCTCTCCA
PRIMER_LEFT_12=2023,23
PRIMER_RIGHT_12=2216,23
PRIMER_LEFT_12_TM=60.096
PRIMER_RIGHT_12_TM=60.617
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MER6 Nonautonomous DNA transposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=20.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=6.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=194
PRIMER_PAIR_PENALTY_13=0.7648
PRIMER_LEFT_13_PENALTY=0.197354
PRIMER_RIGHT_13_PENALTY=0.567488
PRIMER_LEFT_13_SEQUENCE=CGAGAAGAATGGTGACTTTCACT
PRIMER_RIGHT_13_SEQUENCE=AAGTTGGGGGAGATGTTAGACTT
PRIMER_LEFT_13=844,23
PRIMER_RIGHT_13=1104,23
PRIMER_LEFT_13_TM=59.803
PRIMER_RIGHT_13_TM=59.433
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_13_END_STABILITY=6.4000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=261
PRIMER_PAIR_PENALTY_14=0.8358
PRIMER_LEFT_14_PENALTY=0.197354
PRIMER_RIGHT_14_PENALTY=0.638440
PRIMER_LEFT_14_SEQUENCE=CGAGAAGAATGGTGACTTTCACT
PRIMER_RIGHT_14_SEQUENCE=ATAGGCATCGTTGAGAGAGAGGT
PRIMER_LEFT_14=844,23
PRIMER_RIGHT_14=1063,23
PRIMER_LEFT_14_TM=59.803
PRIMER_RIGHT_14_TM=60.638
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=220
PRIMER_PAIR_PENALTY_15=0.8360
PRIMER_LEFT_15_PENALTY=0.346658
PRIMER_RIGHT_15_PENALTY=0.489294
PRIMER_LEFT_15_SEQUENCE=AAGAAGTCTAACATCTCCCCCAA
PRIMER_RIGHT_15_SEQUENCE=GGACAGAAATGAATGACTGTGTG
PRIMER_LEFT_15=1079,23
PRIMER_RIGHT_15=2052,23
PRIMER_LEFT_15_TM=60.347
PRIMER_RIGHT_15_TM=59.511
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=10.0000
PRIMER_RIGHT_15_END_STABILITY=6.4000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=974
PRIMER_PAIR_PENALTY_16=0.8360
PRIMER_LEFT_16_PENALTY=0.346658
PRIMER_RIGHT_16_PENALTY=0.489294
PRIMER_LEFT_16_SEQUENCE=AAGAAGTCTAACATCTCCCCCAA
PRIMER_RIGHT_16_SEQUENCE=TGGACAGAAATGAATGACTGTGT
PRIMER_LEFT_16=1079,23
PRIMER_RIGHT_16=2053,23
PRIMER_LEFT_16_TM=60.347
PRIMER_RIGHT_16_TM=59.511
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MER39 a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=10.0000
PRIMER_RIGHT_16_END_STABILITY=6.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=975
PRIMER_PAIR_PENALTY_17=0.8586
PRIMER_LEFT_17_PENALTY=0.034339
PRIMER_RIGHT_17_PENALTY=0.824213
PRIMER_LEFT_17_SEQUENCE=TGGTGACTTTCACTACAAGCAGA
PRIMER_RIGHT_17_SEQUENCE=ATCCCGCAAAGAAAAAGTAACAG
PRIMER_LEFT_17=853,23
PRIMER_RIGHT_17=1489,23
PRIMER_LEFT_17_TM=59.966
PRIMER_RIGHT_17_TM=60.824
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=6.7000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=637
PRIMER_PAIR_PENALTY_18=0.9367
PRIMER_LEFT_18_PENALTY=0.112479
PRIMER_RIGHT_18_PENALTY=0.824213
PRIMER_LEFT_18_SEQUENCE=AGTCTAACATCTCCCCCAACTTC
PRIMER_RIGHT_18_SEQUENCE=ATCCCGCAAAGAAAAAGTAACAG
PRIMER_LEFT_18=1083,23
PRIMER_RIGHT_18=1489,23
PRIMER_LEFT_18_TM=59.888
PRIMER_RIGHT_18_TM=60.824
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=6.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=407
PRIMER_PAIR_PENALTY_19=0.9367
PRIMER_LEFT_19_PENALTY=0.112479
PRIMER_RIGHT_19_PENALTY=0.824213
PRIMER_LEFT_19_SEQUENCE=AGAAGTCTAACATCTCCCCCAAC
PRIMER_RIGHT_19_SEQUENCE=ATCCCGCAAAGAAAAAGTAACAG
PRIMER_LEFT_19=1080,23
PRIMER_RIGHT_19=1489,23
PRIMER_LEFT_19_TM=59.888
PRIMER_RIGHT_19_TM=60.824
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=1.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, LTR5 LTR from human endogenous retrovirus 5' LTR, clone HERV-K18
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=6.7000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=410
=