PRIMER_SEQUENCE_ID=NM_001789
SEQUENCE=GAACAGCGAAGACAGCGTGAGCCTGGGCCGTTGCCTCGAGGCTCTCGCCCGGCTTCTCTTGCCGACCCGCCACGTTTGTTTGGATTTAATCTTCAGGTTGCCGGCGCCCGCCCGCCCGCTGGCCTCGCGGTGTGAGAGGGAAGCACCCGTGCCTGTGGCTGGTGGCTGGCGCCTGGAGGGTCCGCACACCCGCCCGGCCGCGCCGCTTGCCCGCGGCAGCCGCGTCCCTGAACCGCGGAGTCGTGTTTGTGTTTGACCCGCGGGCGCCGGTGGCGCGCGGCCGAGGCCGGTGTCGGCGGGGCGGGGCGGTCGCGGCGGAGGCAGAGGAAGAGGGAGCGGGAGCTCTGCGAGGCCGGGCGCCGCCATGGAACTGGGCCCGGAGCCCCCGCACCGCCGCCGCCTGCTCTTCGCCTGCAGCCCCCCTCCCGCGTCGCAGCCCGTCGTGAAGGCGCTATTTGGCGCTTCAGCCGCCGGGGGACTGTCGCCTGTCACCAACCTGACCGTCACTATGGACCAGCTGCAGGGTCTGGGCAGTGATTATGAGCAACCACTGGAGGTGAAGAACAACAGTAATCTGCAGAGAATGGGCTCCTCCGAGTCAACAGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCATTGGACAGTAAAGAAAACCTTGAAAATCCTATGAGAAGAATACATTCCCTACCTCAGAAGCTGTTGGGATGTAGTCCAGCTCTGAAGAGGAGCCATTCTGATTCTCTTGACCATGACATCTTTCAGCTCATCGACCCAGATGAGAACAAGGAAAATGAAGCCTTTGAGTTTAAGAAGCCAGTAAGACCTGTATCTCGTGGCTGCCTGCACTCTCATGGACTCCAGGAGGGTAAAGATCTCTTCACACAGAGGCAGAACTCTGCCCCAGCTCGGATGCTTTCCTCAAATGAAAGAGATAGCAGTGAACCAGGGAATTTCATTCCTCTTTTTACACCCCAGTCACCTGTGACAGCCACTTTGTCTGATGAGGATGATGGCTTCGTGGACCTTCTCGATGGAGAGAATCTGAAGAATGAGGAGGAGACCCCCTCGTGCATGGCAAGCCTCTGGACAGCTCCTCTCGTCATGAGAACTACAAACCTTGACAACCGATGCAAGCTGTTTGACTCCCCTTCCCTGTGTAGCTCCAGCACTCGGTCAGTGTTGAAGAGACCAGAACGATCTCAAGAGGAGTCTCCACCTGGAAGTACAAAGAGGAGGAAGAGCATGTCTGGGGCCAGCCCCAAAGAGTCAACTAATCCAGAGAAGGCCCATGAGACTCTTCATCAGTCTTTATCCCTGGCATCTTCCCCCAAAGGAACCATTGAGAACATTTTGGACAATGACCCAAGGGACCTTATAGGAGACTTCTCCAAGGGTTATCTCTTTCATACAGTTGCTGGGAAACATCAGGATTTAAAATACATCTCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGTTTGCCAACCTCATTAAAGAGTTTGTTATCATCGACTGTCGATACCCATATGAATACGAGGGAGGCCACATCAAGGGTGCAGTGAACTTGCACATGGAAGAAGAGGTTGAAGACTTCTTATTGAAGAAGCCCATTGTACCTACTGATGGCAAGCGTGTCATTGTTGTGTTTCACTGCGAGTTTTCTTCTGAGAGAGGTCCCCGCATGTGCCGGTATGTGAGAGAGAGAGATCGCCTGGGTAATGAATACCCCAAACTCCACTACCCTGAGCTGTATGTCCTGAAGGGGGGATACAAGGAGTTCTTTATGAAATGCCAGTCTTACTGTGAGCCCCCTAGCTACCGGCCCATGCACCACGAGGACTTTAAAGAAGACCTGAAGAAGTTCCGCACCAAGAGCCGGACCTGGGCAGGGGAGAAGAGCAAGAGGGAGATGTACAGTCGTCTGAAGAAGCTCTGAGGGCGGCAGGACCAGCCAGCAGCAGCCCAAGCTTCCCTCCATCCCCCTTTACCCTCTTTGCTGCAGAGAAACTTAAGCAAAGGGGACAGCTGTGTGACATTTGGAGAGGGGGCCTGGGACTTCCATGCCTTAAACCTACCTCCCACACTCCCAAGGTTGGAGCCCAGGGCATCTTGCTGGCTACGCCTCTTCTGTCCCTGTTAGACGTCCTCCGTCCATATCAGAACTGTGCCACAATGCAGTTCTGAGCACCGTGTCAAGCTGCTCTGAGCCACAGTGGGATGAACCAGCCGGGGCCTTATCGGGCTCCAGCCATCTCATGAGGGGAGAGGAGACGGAGGGGAGTAGAGAAGTTACACAGAAATGCTGCTGGCCAAATAGCAAAGACAACCTGGGAAGGAAAGGTCTTTGTGGGATAATCCATATGTTTAATTTATTCAACTTCATCAATCACTTTATTTTATTTTTTTTTCTAACTCCTGGAGACTTATTTTACTGCTTCATTAGGTTGAAATACTGCCATTCTAGGTAGGGTTTTATTATCCCAGGGACTACCTCGGCTTTTAATTTAAAAAAAAAAAAGAAGTGGGTAAGAAAATGCAAACCTGTTATAAGTTATCGGACAGAAAGCTAGGTGCTCTGTCACCCCCAGGAGGCGCTGTGGTACTGGGGCTGCTGCTATTTAAGCCAAGAACTGAGGTCCTGGTGAGAGCGTTGGACCCAGGCTTGGCTGCCTGACATAAGCTAAATCTCCCAGACCCACCACTGGCTACCGATATCTATTTGGTGGGAGGTGTGGCCCTGTTCTTCCTCACCCCAGTTCCATGACATTGGCTGGTATAGGAGCCACAGTCAGGAAAGCACTTGAGGCAGCATCTGTTGGGCCACCCCCGGCTCAGTGCTGGAATGTTGCAGTGTAGGTTTCCCAGGGAAGGGGGGTGGGGGTAGGTGGGCTCCACAGGATGGGGGAGGAGCATGTCCACTGAGTATCTTCCTTATGTTGCTGTGATATTGATAGCTTTTATTTTCTAATTTTTAAAAAATGGTCATATTATGAGTCAAAGAGTATCAAATCAGTGTTGGATGGACCACCCAAGGGTGAGGAGAGGGGCTGGAAGCCCTGGGCATTAGGAGAAGGGAGTGGGTGCTGGCATGGACATGACTGGATAGAATTTTCTCAGGAGGGAGCTTGGTGGATTTTGAAGGTAAAACTTTCTGGGTTTATCATGTTTTAATTTTAGAGACAGGGAGTGATGAATCATCACCGGTTGTCCCCTTATCTAACTCCATAAAAGTGGGAATTTCAAAAGAACACCTCATCCAAGGAGCTGGGGCAGACTTCATTGATTCTAGAGAGACCTGTTTCAGTGCCTACTCATCCCTGCCCTCTGGTGCCAGCCTCCTTACCATCACGGCTTCACTGAGGTGTAGGTGGGTTTTTCTTAAACAGGAGACAGTCTCTCCCCTCTTACCTCAACTTCTTGGGGTGGGAATCAGTGATACTGGAGATGGCTAGTTGCTGTGTTACGGGTTTGAGTTACATTTGGCTATAAAACAATCTTGTTGGGAAAAATGTGGGGGAGAGGACTTCTTCCTACACGCGCATTGAGACAGATTCCAACTGGTTAATGATATTGTTTGTAAGAAAGAGATTCTGTTGGTTGACTGCCTAAAGAGAAAGGTGGGATGGCCTTCAGATTATACCAGCTTAGCTAGCATTACTAACCAACTGTTGGAAGCTCTGAAAATAAAAGATCTTGAACCCATAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 42715, GC content failed 2711, low tm 16487, high tm 14506, high any compl 7, high end compl 47, high repeat similarity 64, long poly-x seq 102, ok 8791
PRIMER_RIGHT_EXPLAIN=considered 42685, GC content failed 2750, low tm 16974, high tm 13879, high any compl 3, high end compl 28, high repeat similarity 18, long poly-x seq 110, ok 8923
PRIMER_PAIR_EXPLAIN=considered 945, unacceptable product size 885, high end compl 4, ok 56
PRIMER_PAIR_PENALTY=0.0531
PRIMER_LEFT_PENALTY=0.035468
PRIMER_RIGHT_PENALTY=0.017590
PRIMER_LEFT_SEQUENCE=AAGGTCTTTGTGGGATAATCCAT
PRIMER_RIGHT_SEQUENCE=GGACCTCAGTTCTTGGCTTAAAT
PRIMER_LEFT=2340,23
PRIMER_RIGHT=2641,23
PRIMER_LEFT_TM=59.965
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=302
PRIMER_PAIR_PENALTY_1=0.0601
PRIMER_LEFT_1_PENALTY=0.017590
PRIMER_RIGHT_1_PENALTY=0.042506
PRIMER_LEFT_1_SEQUENCE=ATTTAAGCCAAGAACTGAGGTCC
PRIMER_RIGHT_1_SEQUENCE=ACCCCAAGAAGTTGAGGTAAGAG
PRIMER_LEFT_1=2619,23
PRIMER_RIGHT_1=3426,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.043
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=808
PRIMER_PAIR_PENALTY_2=0.0831
PRIMER_LEFT_2_PENALTY=0.017688
PRIMER_RIGHT_2_PENALTY=0.065377
PRIMER_LEFT_2_SEQUENCE=GAATCAGTGATACTGGAGATGGC
PRIMER_RIGHT_2_SEQUENCE=CTTTCTCTTTAGGCAGTCAACCA
PRIMER_LEFT_2=3429,23
PRIMER_RIGHT_2=3619,23
PRIMER_LEFT_2_TM=59.982
PRIMER_RIGHT_2_TM=59.935
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.0837
PRIMER_LEFT_3_PENALTY=0.018372
PRIMER_RIGHT_3_PENALTY=0.065377
PRIMER_LEFT_3_SEQUENCE=GCTGTGTTACGGGTTTGAGTTAC
PRIMER_RIGHT_3_SEQUENCE=CTTTCTCTTTAGGCAGTCAACCA
PRIMER_LEFT_3=3457,23
PRIMER_RIGHT_3=3619,23
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=59.935
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.1179
PRIMER_LEFT_4_PENALTY=0.075444
PRIMER_RIGHT_4_PENALTY=0.042506
PRIMER_LEFT_4_SEQUENCE=GGATAGAATTTTCTCAGGAGGGA
PRIMER_RIGHT_4_SEQUENCE=ACCCCAAGAAGTTGAGGTAAGAG
PRIMER_LEFT_4=3103,23
PRIMER_RIGHT_4=3426,23
PRIMER_LEFT_4_TM=59.925
PRIMER_RIGHT_4_TM=60.043
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=324
PRIMER_PAIR_PENALTY_5=0.1253
PRIMER_LEFT_5_PENALTY=0.030936
PRIMER_RIGHT_5_PENALTY=0.094334
PRIMER_LEFT_5_SEQUENCE=CTCGATGGAGAGAATCTGAAGAA
PRIMER_RIGHT_5_SEQUENCE=CATCAGTAGGTACAATGGGCTTC
PRIMER_LEFT_5=1027,23
PRIMER_RIGHT_5=1627,23
PRIMER_LEFT_5_TM=59.969
PRIMER_RIGHT_5_TM=59.906
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=601
PRIMER_PAIR_PENALTY_6=0.1408
PRIMER_LEFT_6_PENALTY=0.075444
PRIMER_RIGHT_6_PENALTY=0.065377
PRIMER_LEFT_6_SEQUENCE=GGATAGAATTTTCTCAGGAGGGA
PRIMER_RIGHT_6_SEQUENCE=CTTTCTCTTTAGGCAGTCAACCA
PRIMER_LEFT_6=3103,23
PRIMER_RIGHT_6=3619,23
PRIMER_LEFT_6_TM=59.925
PRIMER_RIGHT_6_TM=59.935
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=2.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=9.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=517
PRIMER_PAIR_PENALTY_7=0.1527
PRIMER_LEFT_7_PENALTY=0.135150
PRIMER_RIGHT_7_PENALTY=0.017590
PRIMER_LEFT_7_SEQUENCE=GACGGAGGGGAGTAGAGAAGTTA
PRIMER_RIGHT_7_SEQUENCE=GGACCTCAGTTCTTGGCTTAAAT
PRIMER_LEFT_7=2272,23
PRIMER_RIGHT_7=2641,23
PRIMER_LEFT_7_TM=60.135
PRIMER_RIGHT_7_TM=60.018
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=24.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=6.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=370
PRIMER_PAIR_PENALTY_8=0.1694
PRIMER_LEFT_8_PENALTY=0.080854
PRIMER_RIGHT_8_PENALTY=0.088584
PRIMER_LEFT_8_SEQUENCE=GAAGCCTTTGAGTTTAAGAAGCC
PRIMER_RIGHT_8_SEQUENCE=GGTCTCCTCCTCATTCTTCAGAT
PRIMER_LEFT_8=793,23
PRIMER_RIGHT_8=1062,23
PRIMER_LEFT_8_TM=59.919
PRIMER_RIGHT_8_TM=60.089
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=270
PRIMER_PAIR_PENALTY_9=0.1770
PRIMER_LEFT_9_PENALTY=0.017590
PRIMER_RIGHT_9_PENALTY=0.159363
PRIMER_LEFT_9_SEQUENCE=ATTTAAGCCAAGAACTGAGGTCC
PRIMER_RIGHT_9_SEQUENCE=ATCTCCAGTATCACTGATTCCCA
PRIMER_LEFT_9=2619,23
PRIMER_RIGHT_9=3448,23
PRIMER_LEFT_9_TM=60.018
PRIMER_RIGHT_9_TM=59.841
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_LEFT_9_END_STABILITY=9.1000
PRIMER_RIGHT_9_END_STABILITY=9.7000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=830
PRIMER_PAIR_PENALTY_10=0.1770
PRIMER_LEFT_10_PENALTY=0.017590
PRIMER_RIGHT_10_PENALTY=0.159363
PRIMER_LEFT_10_SEQUENCE=ATTTAAGCCAAGAACTGAGGTCC
PRIMER_RIGHT_10_SEQUENCE=CATCTCCAGTATCACTGATTCCC
PRIMER_LEFT_10=2619,23
PRIMER_RIGHT_10=3449,23
PRIMER_LEFT_10_TM=60.018
PRIMER_RIGHT_10_TM=59.841
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.1000
PRIMER_RIGHT_10_END_STABILITY=9.7000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=831
PRIMER_PAIR_PENALTY_11=0.2056
PRIMER_LEFT_11_PENALTY=0.017590
PRIMER_RIGHT_11_PENALTY=0.187985
PRIMER_LEFT_11_SEQUENCE=ATTTAAGCCAAGAACTGAGGTCC
PRIMER_RIGHT_11_SEQUENCE=TTCTCTTTAGGCAGTCAACCAAC
PRIMER_LEFT_11=2619,23
PRIMER_RIGHT_11=3617,23
PRIMER_LEFT_11_TM=60.018
PRIMER_RIGHT_11_TM=59.812
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.1000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=999
PRIMER_PAIR_PENALTY_12=0.2057
PRIMER_LEFT_12_PENALTY=0.017688
PRIMER_RIGHT_12_PENALTY=0.187985
PRIMER_LEFT_12_SEQUENCE=GAATCAGTGATACTGGAGATGGC
PRIMER_RIGHT_12_SEQUENCE=TTCTCTTTAGGCAGTCAACCAAC
PRIMER_LEFT_12=3429,23
PRIMER_RIGHT_12=3617,23
PRIMER_LEFT_12_TM=59.982
PRIMER_RIGHT_12_TM=59.812
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.6000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=189
PRIMER_PAIR_PENALTY_13=0.2064
PRIMER_LEFT_13_PENALTY=0.018372
PRIMER_RIGHT_13_PENALTY=0.187985
PRIMER_LEFT_13_SEQUENCE=GCTGTGTTACGGGTTTGAGTTAC
PRIMER_RIGHT_13_SEQUENCE=TTCTCTTTAGGCAGTCAACCAAC
PRIMER_LEFT_13=3457,23
PRIMER_RIGHT_13=3617,23
PRIMER_LEFT_13_TM=59.982
PRIMER_RIGHT_13_TM=59.812
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, reverse L1PA7 3'-end of L1 repeat (subfamily L1PA7) - a consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.4000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=161
PRIMER_PAIR_PENALTY_14=0.2123
PRIMER_LEFT_14_PENALTY=0.030936
PRIMER_RIGHT_14_PENALTY=0.181407
PRIMER_LEFT_14_SEQUENCE=CTCGATGGAGAGAATCTGAAGAA
PRIMER_RIGHT_14_SEQUENCE=GACGACTGTACATCTCCCTCTTG
PRIMER_LEFT_14=1027,23
PRIMER_RIGHT_14=1924,23
PRIMER_LEFT_14_TM=59.969
PRIMER_RIGHT_14_TM=60.181
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=52.174
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=898
PRIMER_PAIR_PENALTY_15=0.2123
PRIMER_LEFT_15_PENALTY=0.030936
PRIMER_RIGHT_15_PENALTY=0.181407
PRIMER_LEFT_15_SEQUENCE=CTCGATGGAGAGAATCTGAAGAA
PRIMER_RIGHT_15_SEQUENCE=TTCAGACGACTGTACATCTCCCT
PRIMER_LEFT_15=1027,23
PRIMER_RIGHT_15=1928,23
PRIMER_LEFT_15_TM=59.969
PRIMER_RIGHT_15_TM=60.181
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, Tigger1 Autonomous DNA transposon
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=902
PRIMER_PAIR_PENALTY_16=0.2123
PRIMER_LEFT_16_PENALTY=0.030936
PRIMER_RIGHT_16_PENALTY=0.181407
PRIMER_LEFT_16_SEQUENCE=CTCGATGGAGAGAATCTGAAGAA
PRIMER_RIGHT_16_SEQUENCE=CTTCAGACGACTGTACATCTCCC
PRIMER_LEFT_16=1027,23
PRIMER_RIGHT_16=1929,23
PRIMER_LEFT_16_TM=59.969
PRIMER_RIGHT_16_TM=60.181
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=8.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=903
PRIMER_PAIR_PENALTY_17=0.2157
PRIMER_LEFT_17_PENALTY=0.127164
PRIMER_RIGHT_17_PENALTY=0.088584
PRIMER_LEFT_17_SEQUENCE=AAGAGGAGCCATTCTGATTCTCT
PRIMER_RIGHT_17_SEQUENCE=GGTCTCCTCCTCATTCTTCAGAT
PRIMER_LEFT_17=721,23
PRIMER_RIGHT_17=1062,23
PRIMER_LEFT_17_TM=59.873
PRIMER_RIGHT_17_TM=60.089
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=342
PRIMER_PAIR_PENALTY_18=0.2215
PRIMER_LEFT_18_PENALTY=0.127164
PRIMER_RIGHT_18_PENALTY=0.094334
PRIMER_LEFT_18_SEQUENCE=AAGAGGAGCCATTCTGATTCTCT
PRIMER_RIGHT_18_SEQUENCE=CATCAGTAGGTACAATGGGCTTC
PRIMER_LEFT_18=721,23
PRIMER_RIGHT_18=1627,23
PRIMER_LEFT_18_TM=59.873
PRIMER_RIGHT_18_TM=59.906
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=907
PRIMER_PAIR_PENALTY_19=0.2247
PRIMER_LEFT_19_PENALTY=0.159363
PRIMER_RIGHT_19_PENALTY=0.065377
PRIMER_LEFT_19_SEQUENCE=GGGAATCAGTGATACTGGAGATG
PRIMER_RIGHT_19_SEQUENCE=CTTTCTCTTTAGGCAGTCAACCA
PRIMER_LEFT_19=3427,23
PRIMER_RIGHT_19=3619,23
PRIMER_LEFT_19_TM=59.841
PRIMER_RIGHT_19_TM=59.935
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.6000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=193
=